REVIEW ARTICLE / PREGLEDNI ČLANAK
Ovsynch based protocols in
reproductive management and
infertility treatment in dairy
cows - when and why?
P. Meglić, B. Špoljarić*, G. Štibrić, M. Samardžija, M. Lojkić, N. Prvanović
Babić, N. Maćešić, T. Karadjole, J. Šavorić, I. Folnožić, J. Grizelj, I. Butković,
D. Gereš and S. Vince
Abstract
In order to optimise reproductive
performance and thus production, it is now
impossible to control the reproduction of
dairy cows without the use of hormones.
Due to the characteristics of dairy cows,
the use of hormones not only reduces the
need for visual heat detection and thus the
number of undetected cows in heat, but also
prevents certain problems associated with
intensive production and its negative effects
on reproduction. Ovsynch, as a planned
combination of GnRH and PGF2α that allows
artificial insemination at the optimal time
without the need to control ovaries and uterus,
once offered solutions to these problems, but
over time its shortcomings were recognised.
Therefore, pre-synchronisation protocols have
been developed to create optimal conditions
for Ovsynch and allow for the best outcome.
In addition to pre-synchronisation, Ovsynch
and its modifications can also be used in
the resynchronisation of inseminated and
sonographically diagnosed non-pregnant cows
as soon as possible, improving farm efficiency.
The addition of progesterone implants also
further improves the performance of Ovsynchbased protocols. In addition to controlling
reproduction, Ovsynch and its modifications
have also proven successful in treating certain
forms of infertility in dairy cows, such as
anovulatory conditions and cystic ovarian
disease. This paper presents the possibilities
of using the Ovsynch protocol and its various
modifications, and their advantages and
disadvantages.
Key words: dairy cow; Ovsynch; presynchronisation; resynchronisation; GnRH; PGF2α
Patrik MEGLIĆ, DVM, Assistant, School of Medicine, University of Zagreb, Zagreb, Croatia; Branimira
ŠPOLJARIĆ*, DVM, PhD, Assistant Professor, (Corresponding author, e-mail: bzevrnja@vef.unizg.hr),
Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia; Goran ŠTIBRIĆ, DVM, PhD, Dairy
farm “Zdenačka farma d.o.o“, Veliki Zdenci, Croatia; Marko SAMARDŽIJA, DVM, PhD, Full Professor,
Martina LOJKIĆ, DVM, PhD, Associate Professor, Nikica PRVANOVIĆ BABIĆ, DVM, PhD, Full Professor,
Nino MAĆEŠIĆ, DVM, PhD, Associate Professor, Tugomir KARADJOLE, DVM, PhD, Full Professor, Juraj
ŠAVORIĆ, DVM, Assistant, Ivan FOLNOŽIĆ, DVM, PhD, Associate Professor, Juraj GRIZELJ, DVM, PhD,
Full Professor, Ivan BUTKOVIĆ, DVM, PhD, Postdoc, Darko GEREŠ, DVM, PhD, Retired Full Professor,
Silvijo VINCE, DVM, PhD, Associate Professor, Faculty of Veterinary Medicine, University of Zagreb,
Zagreb, Croatia
VETERINARSKA STANICA 54 (2), 2023.
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https://doi.org/10.46419/vs.54.2.8
213
P. MEGLIĆ, B. ŠPOLJARIĆ, G. ŠTIBRIĆ, M. SAMARDŽIJA,
M. LOJKIĆ, N. PRVANOVIĆ BABIĆ, N. MAĆEŠIĆ, T. KARADJOLE, J. ŠAVORIĆ, I. FOLNOŽIĆ, J. GRIZELJ, I. BUTKOVIĆ, D. GEREŠ and S. VINCE
Introduction
In modern dairy farming, satisfactory
production cannot be achieved without
optimal reproduction. In recent decades,
several protocols have been routinely
used in reproduction management on
dairy farms (Pursley et al., 1997; Morini
et al., 2019), with the aim of minimising
and/or avoiding the most stressful
events in production (oestrus detection,
treatment of postpartum anoestrus,
shortening the calving interval) (Lojkić et
al., 2018; Dobos et al., 2022). Most of these
protocols are based on the administration
of hormones for oestrus induction and
synchronisation of ovulation, allowing
for fixed time artificial insemination
(FTAI). The most commonly used
hormones are synthetic analogues
of gonadotropin releasing hormone
(GnRH) in combination with natural or
synthetic analogues of prostaglandin
F2α (PGF2α). In short, GnRH has a role to
stimulate follicular growth, development
and ovulation, while PGF2α, which has a
luteolytic effect, enables the development
of a new oestrous cycle. The aim of this
paper is to give a brief overview of the
protocols used from professional point of
view.
Synchronisation of ovulation
The
best-known
protocol
for
oestrus synchronisation based on the
administration of GnRH and PGF2α
is Ovsynch (Pursley et al., 1995). This
protocol was developed with the goal
of tight synchronisation of ovulation, by
synchronising follicular development,
in a way that allows for a single timed
artificial insemination (TAI) with
satisfactory conception rates (Laven,
2019). The protocol begins with the
first GnRH administration randomly
during the oestrous cycle, followed
seven days later by PGF2α. The second
GnRH is administered two days after
PGF2α, allowing for FTAI 16 (8-24) hours
214
after the last GnRH administration
(Kasimanickam, 2015). The role of the
first GnRH is to induce ovulation of the
follicle present in the ovary at the time,
leading to corpus luteum (CL) formation.
Efficiency in inducing ovulation varies
from 66–85%, and depends on the
stage of follicular development at the
time of administration. The role of the
PGF2α administered 7 days later is to
cause luteolysis, thus enabling further
development of a new follicular wave
that started when ovulation of the
present follicle was induced (Nowicki
et al., 2017). The role of the second
GnRH, administered on the 9th day is
to synchronise the event of ovulation
of the dominant follicle of this new
follicular wave (Fricke, 2001) that started
to develop after the first GnRH. Artificial
insemination (AI) is performed 16-24
hours after the second GnRH (Nowicki et
al., 2017). This protocol is most efficient in
lactating cows, less efficient in anoestrus
cows and least efficient in heifers (Laven,
2019). The greatest advantage of Ovsynch
is the tight ovulation synchronisation,
allowing for AI without prior oestrus
detection (Kasimanickam, 2015). Oestrus
detection is a growing problem in
modern dairy farms, affecting 10-40% of
farms. The reason may be poor oestrus
expression, though the shortening of
oestrus and weakening of signs of heat
are common in high yielding dairy cows.
This is likely due to the negative energy
balance and correlated metabolic and
endocrinologic disturbances (Brozos
et al., 2021; Sangsritavong et al., 2002),
including lower LH and oestrogen
concentrations.
Synchronization
protocols like Ovsynch offer a solution
for this kind of reproductive problem,
by enabling AI without previous oestrus
detection (Nowicki et al., 2017).
Furthermore, Ovsynch could provide
a solution for farms affected with heat
VETERINARSKA STANICA 54 (2), 213-222, 2023.
Ovsynch based protocols in reproductive management and infertility treatment in dairy cows - when and why?
Ovsynch i njegove modifikacije u kontroli reprodukcije i liječenju određenih neplodnosti u mliječnih krava - kada i zašto?
stress, as one of the causative factors
for decreased reproductive efficiency
(Nowicki et al., 2017). However, Ovsynch
has some shortcomings, even in lactating
cows, where up to 40% of animals do not
respond to this protocol, either due to
insufficient synchronisation of follicular
waves, or failed luteolysis (Martins
and Pursley, 2016). In the first case, in
cows whose dominant follicles do not
respond to the first GnRH, the reason
is probably a follicular age of 3-4 days
of at the time of the first GnRH, when
there is a possibility of their submission
to atresia before PGF2α administration.
In this situation, the new emerging
follicular wave with new dominant
follicle will not respond with ovulation
at the second GnRH administration
(Laven, 2019.). The solution is presynchronisation of cows, which should
result with cows in days 5 to 9 of the
cycle at the first GnRH administration
(Vasconselos et al., 1999; Bello et al.,
2006). The second problem encountered
in Ovsynch is a failed response of CL to
PGF2α application. The lack of luteolytic
effect results in suppressed growth of the
second follicular wave, thus disabling
synchronisation of ovulation and FTAI
(Nowicki et al., 2017.). Percentage of
cows in which luteolysis was not induced
varied between 10–11%. This number
can be lowered by the application of
the second PGF2α 24h after the first
application (Laven, 2019).
The greatest results are obtained
when Ovsynch is used at the herd
level. This strategy allows synchronous
insemination of a large number of
postpartum cows and increases the
pregnancy rate in the herd. When used
on a single, problematic animal, increase
in pregnancy rates cannot be expected
(Nowicki et al., 2017). The second
shortcoming of Ovsynch is decreased
success in improving pregnancy rates
in heifers, especially when compared
to insemination at observed oestrus.
VETERINARSKA STANICA 54 (2), 213-222, 2023.
For this reason, heifers are submitted to
modified protocols (Laven, 2019) (Fig. 1).
One Ovsynch modification is Cosynch, a
protocol based on Ovsynch in which the
animals are inseminated at the time the
second GnRH is applied. In this way, the
animals are handled one time less, which
is very important from the management
perspective (Rabiee et al., 2005); however,
the results obtained in dairy cows are
not optimal (Fricke, 2001). The Select
Synch protocol is similar to Cosynch,
but lacks the final GnRH administration
awaiting the onset of oestrus (Rabiee et
al., 2005). The overall success of Ovsynch
and Select Synch is very similar, though
Select Synch has the (dis)advantage of
depending on oestrus detection for AI
(Rabiee et al., 2005).
Figure 1. Ovsynch and its modifications
TAI- timed artificial insemination; AI- artificial
insemination
Pre-synchronisation protocols (Fig. 2)
have been developed as a modification
of the original Ovsynch protocol, due
to the fact that the protocol results are
influenced by the phase of the oestrous
cycle in which synchronisation starts
(Fricke, 2001; Rabiee et al., 2005). Ovsynch
programs that start too early, i.e., 1-4
days after ovulation, or too late, i.e., 13-
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P. MEGLIĆ, B. ŠPOLJARIĆ, G. ŠTIBRIĆ, M. SAMARDŽIJA,
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20 days after ovulation, result in fewer
synchronised animals. Furthermore,
depending on the domination phase of
the preovulatory follicle (before or after
domination phase) at the time of the first
GnRH application, ovulation might not
be achieved, and thus no synchronous
new wave will be obtained. The best
response is achieved when the first GnRH
is applied between 5th and 9th day of
cycle (Vasconcelos et al., 1999; Rabiee et
al., 2005). Pre-synchronisation protocols
have a role of ensuring this phase of
cycle for the beginning of Ovsynch
(Kasimanickam, 2015).
One of them, Presynch-Ovsynch,
is based on administration of PGF2α to
synchronise the cycles for Ovsynch.
Presynch begins with the administration
of PGF2α 12 days before the scheduled
Ovsynch, synchronising ovarian activity
and ensuring the presence of second
wave follicles in the ovaries at the time
of the first GnRH application (Moreira
et al., 2001). Several authors suggest that
the Presynch should consist of two PGF2α
administrations 14 days apart, with
the second given 10-14 days before the
planned Ovsynch (Moreira et al., 2001;
El-Zarkouny et al., 2004; Navanukraw et
al., 2004; Galvao et al., 2007). Dirandeh
et al. (2015) recommend the onset of
Ovsynch 7 days after the second PGF2α
at pre-synchronisation. The pregnancy
rates achieved are 8% higher when presynchronisation is used (Gumen et al.,
2012), while El-Zarkouny et al. (2004)
found a pregnancy rate of 48.8% in
pre-synchronised animals, compared
to 37.5% in cows synchronised with
Ovsynch only.
The G6G protocol begins with PGF2α
application, which serves to luteolise the
functional CL. Two days later, GnRH
is administered to induce ovulation
(Bello, et al., 2006). Their joint task is to
initiate a new oestrous cycle. Ovsynch
is then started 6 days after GnRH, when
cows should be in day 6 of the cycle. A
dominant follicle able to ovulate after the
first GnRH of Ovsynh is expected in the
ovaries of cows synchronised in this way
(Bello et al., 2006; Kasimanickam, 2015).
The Double Ovsynch is a modification
of the basic Ovsynch, in which two
Ovsynch protocols are applied 7 days
apart (Souza et al., 2008). FTAI follows
after the second Ovsynch. Compared
to Presynch, Double Ovsynch results
in higher pregnancy rates (49.7% and
Figure 2. Some pre-synchronisation protocols based on Ovsynch
TAI- timed artificial insemination; AI- artificial insemination
216
VETERINARSKA STANICA 54 (2), 213-222, 2023.
Ovsynch based protocols in reproductive management and infertility treatment in dairy cows - when and why?
Ovsynch i njegove modifikacije u kontroli reprodukcije i liječenju određenih neplodnosti u mliječnih krava - kada i zašto?
41%, respectively). The explanation
could be that cows with postpartum
inactive ovaries do not respond to PGF2α
application in Presynch. On the other
hand, two additional GnRH applications
in Double Ovsynch stimulate the ovaries
to return to cyclic activity. In cows with
normally active ovaries, Double Ovsynch
had no negative effect. Furthermore,
Double Ovsynch achieved better results
in heifers than cows (65.2% and 37.5%,
respectively). Therefore, Double Ovsynch
is recommended for use in heifers,
while other PGF2α pre-synchronisation
protocols, such as Presynch, are
recommended for cows (Nowicki et al.,
2017).
Progesterone supplementation for
a duration of 7 days, starting with the
first GnRH and ending with PGF2α
application in Ovsynch, can be used for
oestrus induction and synchronisation of
ovulation in cows and heifers (Brozos et
al., 2021). They are most commonly used
in anoestrus cows and heifers, and in
cows with cystic ovarian disease (COD)
(Kasimanickam,
2015).
Intravaginal
application of controlled internal drug
release (CIDR) or progesterone releasing
intravaginal device (PRID) releases a
certain amount of progesterone, which
is sufficient for oestrus appearance
in cows after removal of the device
(Fricke, 2001). McDougall (2010) showed
that the vast majority of cows showed
no signs of oestrus at the beginning
of spring breeding season, and 73%
had no detectable CL at the start of
the synchronisation programme. In
addition, both Ovsynch and Ovsynch
with progesterone supplementation
were found to reduce the number of days
open, while progesterone supplemented
Ovsynch reduced the number of days
open for 6 days more than with basic
Ovsynch. Therefore, Ovsynch with
progesterone supplementation is a good
solution for herds where anoestrus cows
are a significant problem (Laven, 2019).
Resynchronisation protocols can be
used for cows that fail to conceive to TAI,
(Parkinson, 2019) (Fig. 3). The key role in
dairy farm management is to accurately
identify these animals. Early pregnancy
diagnostics should focus on detecting
non-pregnant cows. Their subsequent
submission to the next AI procedure
increases reproductive efficiency and
pregnancy rates, and shortens the period
between two AIs (Fricke, 2001). Ovsynch
can also be used for resynchronisation and
allows AI 33-40 days after the previous
AI, after the cow has been diagnosed as
non-pregnant. This period can be further
Figure 3. Resynchronisation protocols based on Ovsynch
US- ultrasound pregnancy detection; TAI- timed artificial insemination
VETERINARSKA STANICA 54 (2), 213-222, 2023.
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P. MEGLIĆ, B. ŠPOLJARIĆ, G. ŠTIBRIĆ, M. SAMARDŽIJA,
M. LOJKIĆ, N. PRVANOVIĆ BABIĆ, N. MAĆEŠIĆ, T. KARADJOLE, J. ŠAVORIĆ, I. FOLNOŽIĆ, J. GRIZELJ, I. BUTKOVIĆ, D. GEREŠ and S. VINCE
shortened, by administering GnRH 7
days before the planned sonographic
examination. In this way, PGF2α can be
administered on the day of pregnancy
diagnosis in non-pregnant cows, and
these cows can be inseminated in three
days (Špoljarić et al., 2017). Pregnancy
rates obtained when resynchronisation
starts between 23 and 25 days after the
previous Al vary between 23 and 50%,
while pregnancy rates drop to 25.2 to
33.6% when it starts between 30 and 32
days post-AI (Nowicki et al., 2017). PRID
and CIDR devices can also be used for
oestrus synchronisation in cows that
have failed to conceive after the first
TAI. One protocols using progesterone
is the Fastback programme, where
progesterone devices are inserted 14
days after TAI, removed 7 days later (21
days after TAI), and cows are observed
between 22 and 25 days after TAI for
possible signs of oestrus. This increases
the number of non-pregnant cows that
are re-inseminated after failed conception
at the first AI (Laven, 2019).
Use in heifers and cows
Compared to AI at observed oestrus,
where the conception rates achieved
are between 50-60%, Ovsynch in heifers
results in lower conception rates, usually
between 30–40% and FTAI is not so readily
used. The reason for this is the different
follicular dynamics between cows and
heifers, for in the latter, oestrus is visible
very soon after PGF2α application,
resulting in asynchronous ovulation for
FTAI (Risco, 2015). Therefore, modified
protocols are required (Laven, 2019).
Double Ovsynch is one of the protocols
that can be used to prevent asynchronous
ovulation (Nowicki et al., 2017).
Furthermore, PRID or CIDR devices in
combination with Ovsynch modification
result in an increased conception
rate, compared to observed oestrus
insemination after PGF2α application
218
(57% and 48%, respectively) (McDougall
et al., 2013), but only when heifers were
inseminated together with the second
GnRH administration. Similar results are
obtained with administration of Cosynch
in heifers (Laven, 2019), although
conception rates may be 3 to 4% lower,
the advantage being one less animal
handling (Colazo and Mapletoft, 2014).
Conception rates in heifers using the
Cosynch modification where the CIDR
device is administered at the time of the
first GnRH administration, and removed
5 days later with PGF2α application, and
the second GnRH is administered 72 h
later at the same time as AI, vary between
50 and 60% (Risco, 2015). The combination
of progesterone devices and PGF2α is also
used. In these protocols, the progesterone
device is administered at the beginning
of the protocol, PGF2α is applied on
day 6, and the progesterone device is
removed one day later. Subsequent AI
can be performed once at 56 hours, or
twice at 48 and 72 hours after removal
of the progesterone device (Laven, 2019).
When comparing these two programmes
(Cosynch and progesterone-PGF2α), no
difference in pregnancy rates is observed
(52.4 vs. 54.8%, respectively) (Sahu et al.,
2015). The price of progesterone-PGF2α is
much lower compared to Cosynch, but
Cosynch requires one less handling of
the animal (3 and 4, respectively). These
two variables must therefore be taken
into account when selecting appropriate
protocols for each individual farm
(Colazo and Mapletoft, 2014; Laven,
2019).
Use in fertility problems
Anoestrus occurs physiologically in
cows after parturition. This condition
is considered a multi-causal pathology
when ovarian cyclic activity does not
return in the expected time when animals
need to be bred again (Parkinson, 2019),
when treatment is required. Hormonal
VETERINARSKA STANICA 54 (2), 213-222, 2023.
Ovsynch based protocols in reproductive management and infertility treatment in dairy cows - when and why?
Ovsynch i njegove modifikacije u kontroli reprodukcije i liječenju određenih neplodnosti u mliječnih krava - kada i zašto?
treatment aims to cause a rebound
effect on the pituitary gland so that
cyclic activity resumes, and in most
cases, progesterone is used. However,
hormones that cause secretion of
gonadotropins, or have a gonadotropic
effect themselves can also be used for this
purpose (Parkinson, 2019). Ovsynch has
decreased efficiency in anoestrus cows
than in cows with normal ovarian activity
for several reasons: follicular response to
the first GnRH is reduced, progesterone
concentration is lower (partly because
ovulation does not occur after the first
GnRH, partly because endogenous CL is
lacking in anoestrus cows) and there is a
risk of a short luteal phase, even in cows
where synchronous ovulation is achieved.
Some modified pre-synchronisation
protocols, such as G6G, have resulted
in better conception rates in anoestrus
cows then Ovsynch alone (Colazo and
Mapletoft, 2014). The best results are
obtained when Ovsynch is combined
with progesterone devices, administered
between days 0 and 7 of the Ovsynch
protocol. Equine chorionic gonadotropin
(eCG), can be administered concurrently
with PGF2α to better stimulate the
growth of dominant follicles, especially
in deep anoestrus cows (Bryan et al.,
2013). This modification of progesterone
supplemented Ovsynch has proven to be
very efficient in anoestrus cows that are
not in optimal body condition (Laven,
2019).
The second problem occurring in
dairy cows is cystic ovarian disease
(COD). Both GnRH and PGF2α can be
used for treatment of COD, depending
on the type of the cyst (Parkinson,
2019). Therefore, Ovsynch can be used
in treatment of COD (Nowicki et al.,
2017). GnRH is expected to increase LH
secretion from pituitary gland, resulting
in luteinisation of cyst or in ovulation
of new follicles. Sometimes, the cyst
itself can rupture. The following PGF2α
administration results in lysis of luteal
VETERINARSKA STANICA 54 (2), 213-222, 2023.
tissue. The second GnRH is expected
to stimulate the follicles of previously
induced follicular wave to ovulate, thus
enabling FTAI of the treated animal.
However, conception rates obtained after
Ovsynch are not satisfying, and are 19%,
as shown in Parkinson (2019). When
Ovsynch modification, comprised of
GnRH and PGF2α on Day 0, PGF2α on Day
14 and GnRH on Day 16, is used, results
of oestrus detection, first AI conception
rates and pregnancy rates obtained can be
compared to those in healthy animals, and
exceed those obtained with Ovsynch only
(Gundling et al., 2015), with conception
rates of 35% in modified Ovsynch and
19% in Ovsynch, respectively (Parkinson,
2019).
The second GnRH in Ovsynch
can be replaced by human chorionic
gonadotropin (hCG). DeRensis et al.
(2008) observed a faster disappearance
of ovarian cysts in this Ovsynch
modification. Similar results were
presented by other authors, indicating
that this replacement could be a solution
for improving Ovsynch efficiency for
COD treatment, probably due to longer
acting and better clinical effect of hCG
compared to GnRH (Kinser et al., 1983).
For treatment of luteal cysts, only
PGF2α can be used (Jeengar et al., 2014;
Parkinson et al., 2019). In more than 80%
of cows treated with PGF2α, the cysts
disappear and oestrus occurs within 3 to
5 days after PGF2α application (Dobson et
al., 1977; Statham, 2016). The percentage
of pregnant cows at first oestrus was
66% (Brito and Palmer, 2004). If the cow
diagnosed with luteal cyst does not
respond to PGF2α, it is most likely that the
diagnosis was incorrect (Parkinson et al.,
2019).
Conclusion
The implementation of hormonal
protocols
that
enable
oestrus
synchronisation
opens
up
many
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P. MEGLIĆ, B. ŠPOLJARIĆ, G. ŠTIBRIĆ, M. SAMARDŽIJA,
M. LOJKIĆ, N. PRVANOVIĆ BABIĆ, N. MAĆEŠIĆ, T. KARADJOLE, J. ŠAVORIĆ, I. FOLNOŽIĆ, J. GRIZELJ, I. BUTKOVIĆ, D. GEREŠ and S. VINCE
opportunities
for
reproductive
management. The greatest advantage
of certain protocols is the possibility of
using AI at a predetermined time, which
allows for less frequent handling of
animals and improved time efficiency.
An additional advantage of such
protocols is the reduction of problems
that occur when oestrus is not detected.
Ovsynch is one of the most popular
hormonal protocols based on the use
of GnRH and PGF2α. This method is
much more effective when used in cows
than in heifers. In order to improve
the pregnancy rates after the Ovsynch
protocol, some modifications of the
basic protocol are being investigated.
Such modifications should address the
problems that arise when developing
follicles or corpus luteum do not
respond to GnRH or PGF2α application.
Another drawback of the protocol that
should be highlighted is the weak effect
on the corpus luteum, but this could
be overcome by using progesterone
devices in the form of spirals or implants
(Nowicki et al., 2017). In addition to
routine cycle synchronization, the
protocol can also be used in the treatment
of ovarian cystic disease, silent heat, and
heat stress.
5.
6.
7.
8.
9.
10.
11.
12.
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221
P. MEGLIĆ, B. ŠPOLJARIĆ, G. ŠTIBRIĆ, M. SAMARDŽIJA,
M. LOJKIĆ, N. PRVANOVIĆ BABIĆ, N. MAĆEŠIĆ, T. KARADJOLE, J. ŠAVORIĆ, I. FOLNOŽIĆ, J. GRIZELJ, I. BUTKOVIĆ, D. GEREŠ and S. VINCE
Ovsynch i njegove modifikacije u kontroli reprodukcije i liječenju
određenih neplodnosti u mliječnih krava - kada i zašto?
Patrik MEGLIĆ, dr. med. vet., asistent, Medicinski fakultet Sveučilišta u zagrebu, Zagreb,
Hrvatska; dr. sc. Branimira ŠPOLJARIĆ, dr. med. vet., docentica, Veterinarski fakultet Sveučilišta
u Zagrebu, Zagreb, Hrvatska; dr. sc. Goran ŠTIBRIĆ, dr. med. vet., Zdenačka farma d.o.o, Veliki
Zdenci, Hrvatska; dr. sc. Marko SAMARDŽIJA, dr. med. vet., redoviti profesor, dr. sc. Martina
LOJKIĆ, dr. med. vet., izvanredna profesorica, dr. sc. Nikica PRVANOVIĆ BABIĆ, dr. med. vet.,
redovita profesorica, dr. sc. Nino MAĆEŠIĆ, dr. med. vet., izvanredni profesor, dr. sc. Tugomir
KARADJOLE, dr. med. vet., redoviti profesor, Juraj ŠAVORIĆ, dr. med. vet., asistent, dr. sc.
Ivan FOLNOŽIĆ, dr. med. vet., izvanredni profesor, dr. sc. Juraj GRIZELJ, dr. med. vet., redoviti
profesor, dr. sc. Ivan BUTKOVIĆ, dr. med. vet, poslijedoktorand, dr. sc. Darko GEREŠ, dr. med.
vet, umirovljeni profesor, dr. sc. Silvijo VINCE, dr. med. vet., izvanredni profesor, Veterinarski
fakultet Sveučilišta u Zagrebu, Zagreb, Hrvatska
Da bi se optimizirala reproduktivna
učinkovitost, a samim time i proizvodnja
mlijeka, danas je nemoguće zamisliti kontrolu
reprodukcije mliječnih krava bez uporabe
hormona. Zbog osobitosti mliječnih krava,
uporabom hormona smanjuje se potreba za
vizualnim opažanjem estrusa i na taj način
broj nedetektiranih krava u estrusu, ali se
i sprečavaju određeni problemi vezani za
intenzivnu proizvodnju i njihov negativan
utjecaj na reprodukciju. Ovsynch je kao
planirana kombinacija GnRH i PGF2α koja
omogućuje
umjetno
osjemenjivanje
u
optimalno vrijeme bez potrebe za kontrolom
jajnika i maternice, svojevremeno ponudio
rješenje vezano za ove probleme, ali s
vremenom su uočeni i njegovi nedostatci. Stoga
su se razvili i presinkronizacijski protokoli
koji omogućavaju optimalne preduvjete
222
za Ovsynch i omogućuju njegov najbolji
rezultat. Osim u presinkronizaciji, Ovsynch
i njegove modifikacije mogu se koristiti i u
resinkronizaciji osjemenjenih i potom, čim
prije, dijagnosticiranih negravidnih krava,
čime se poboljšava učinkovitost farme.
Dodavanje progesteronskih implantanata
dodatno poboljšava i uspješnost protokola
koji se baziraju na Ovsynchu. Osim u
kontroli reprodukcije, Ovsynch i njegove
modifikacije su se pokazale uspješnima i u
liječenju određenih neplodnosti u mliječnih
krava, poput anovulatornih stanja i cistične
bolesti jajnika. U ovom su radu prikazane
mogućnosti uporabe Ovsynch protokola i
njegovih različitih modifikacija, zajedno s
njihovim prednostima i nedostatcima.
Ključne riječi: mliječne krave, Ovsynch,
presinkronizacija, resinkronizacija, GnRH, PGF2α
VETERINARSKA STANICA 54 (2), 213-222, 2023.