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WO2009154369A3 - 오메가-3계 고도불포화 지방산의 고순도 정제방법 - Google Patents

오메가-3계 고도불포화 지방산의 고순도 정제방법 Download PDF

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Publication number
WO2009154369A3
WO2009154369A3 PCT/KR2009/002958 KR2009002958W WO2009154369A3 WO 2009154369 A3 WO2009154369 A3 WO 2009154369A3 KR 2009002958 W KR2009002958 W KR 2009002958W WO 2009154369 A3 WO2009154369 A3 WO 2009154369A3
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WO
WIPO (PCT)
Prior art keywords
genus
fatty acid
omega
purification method
highly unsaturated
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Application number
PCT/KR2009/002958
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English (en)
French (fr)
Other versions
WO2009154369A2 (ko
Inventor
김갑진
손홍주
황우성
구윤모
김진일
양진효
Original Assignee
에이케이바이오텍 주식회사
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by 에이케이바이오텍 주식회사 filed Critical 에이케이바이오텍 주식회사
Priority to US12/999,177 priority Critical patent/US20110091947A1/en
Publication of WO2009154369A2 publication Critical patent/WO2009154369A2/ko
Publication of WO2009154369A3 publication Critical patent/WO2009154369A3/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C51/00—Preparation of carboxylic acids or their salts, halides or anhydrides
    • C07C51/42—Separation; Purification; Stabilisation; Use of additives
    • C07C51/487—Separation; Purification; Stabilisation; Use of additives by treatment giving rise to chemical modification

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • General Chemical & Material Sciences (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Fats And Perfumes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

본 발명은 오메가-3계 고도불포화 지방산의 고순도 정제방법에 관한 것으로, 구체적으로는 a) 캔디다(Candida) 속, 리조퍼스(Rhizopus) 속, 무코(Mucor) 속, 아스퍼질러스(Aspergillus) 속 및 수도모나스(Pseudomonas) 속으로 이루어진 군에서 선택되는 하나이상의 미생물에서 추출된 효소촉매 존재하에서 천연유지를 에탄올을 이용 에탄올리시스(ethanolysis)하여 지방산 에틸 에스테르(Fatty acid ethyl ester; FAEE)를 제조하는 단계;b) 상기 제조된 지방산 에틸 에스테르를 100 내지 200℃ 및 0.001 내지 10mmHg에서 단증류(Short Path Distillation;SPD) 장치를 이용하여 예비증류하는 단계;c) 상기 예비증류를 거친 에틸 에스테르를 100 내지 200℃ 및 0.001 내지 10mmHg에서 감압분별증류하여 농축지방산이 형성되는 단계; d) 상기 농축지방산을 모사 이동상(Simulated Moving Bed;SMB) 컬럼 크로마토그래피를 통해 정제하는 단계;를 포함하는 오메가-3계 고도불포화 지방산의 고순도 정제방법을 포함하여 환경친화적이면서도 용이하게 이용할 수 있는 오메가-3계 고도불포화 지방산의 고순도 정제방법에 관한 것이다.
PCT/KR2009/002958 2008-06-20 2009-06-03 오메가-3계 고도불포화 지방산의 고순도 정제방법 WO2009154369A2 (ko)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US12/999,177 US20110091947A1 (en) 2008-06-20 2009-06-03 High-Purity Purification Method for Omega-3 Highly Unsaturated Fatty Acids

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2008-0058114 2008-06-20
KR1020080058114A KR101357298B1 (ko) 2008-06-20 2008-06-20 오메가-3계 고도불포화 지방산의 고순도 정제방법

Publications (2)

Publication Number Publication Date
WO2009154369A2 WO2009154369A2 (ko) 2009-12-23
WO2009154369A3 true WO2009154369A3 (ko) 2010-03-11

Family

ID=41434532

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2009/002958 WO2009154369A2 (ko) 2008-06-20 2009-06-03 오메가-3계 고도불포화 지방산의 고순도 정제방법

Country Status (3)

Country Link
US (1) US20110091947A1 (ko)
KR (1) KR101357298B1 (ko)
WO (1) WO2009154369A2 (ko)

Families Citing this family (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2591778B1 (en) 2009-12-30 2021-01-20 BASF Pharma (Callanish) Limited Polyunsaturated fatty acid compositions obtainable by a simulated moving bed chromatographic separation process
KR101173289B1 (ko) 2010-05-04 2012-08-10 (주)산엔들 초임계 이산화탄소 추출을 이용하여 식물로부터 오메가 지방산 함유 추출물의 제조방법
GB201111601D0 (en) 2011-07-06 2011-08-24 Equateq Ltd New process
GB201111595D0 (en) * 2011-07-06 2011-08-24 Equateq Ltd Improved process
GB201111589D0 (en) 2011-07-06 2011-08-24 Equateq Ltd New modified process
GB201111591D0 (en) 2011-07-06 2011-08-24 Equateq Ltd Further new process
GB201111594D0 (en) 2011-07-06 2011-08-24 Equateq Ltd New improved process
PE20190969A1 (es) 2012-05-14 2019-07-08 Nippon Suisan Kaisha Ltd Acido graso altamente insaturado o ester etilico de acido graso altamente insaturado, con reducidos contaminantes ambientales, y metodo para producir el mismo
JP2015523333A (ja) * 2012-05-16 2015-08-13 コシードバイオファーム カンパニー リミテッド 魚類眼球の破砕物又は抽出物を含有する化粧料、薬学及び食品組成物
FR2991337B1 (fr) * 2012-05-29 2016-09-02 Roquette Freres Procede continu d'enrichissement en esters ethyliques de dha d'une huile produite par des microalgues
GB201300354D0 (en) * 2013-01-09 2013-02-20 Basf Pharma Callanish Ltd Multi-step separation process
US9428711B2 (en) 2013-05-07 2016-08-30 Groupe Novasep Chromatographic process for the production of highly purified polyunsaturated fatty acids
PE20200389A1 (es) * 2013-05-07 2020-02-24 Groupe Novasep Proceso cromatografico para la produccion de acidos grasos poliinsaturados altamente purificados
EP2801604B1 (en) 2013-05-07 2017-04-12 Groupe Novasep Chromatographic process for the production of highly purified polyunsaturated fatty acids
US8802880B1 (en) 2013-05-07 2014-08-12 Group Novasep Chromatographic process for the production of highly purified polyunsaturated fatty acids
JP2015030685A (ja) * 2013-07-31 2015-02-16 備前化成株式会社 逆相カラムを用いる擬似移動層クロマトグラフィーによる脂溶性物質の分離法およびそのための装置
EP3029021A1 (en) * 2013-07-31 2016-06-08 Bizen Chemical Co., Ltd. Method for separating fat-soluble material by simulated moving bed chromatography, and device for same
JP2015031538A (ja) * 2013-07-31 2015-02-16 備前化成株式会社 順相カラムを用いる擬似移動層クロマトグラフィーによる脂溶性物質の分離法およびそのための装置
FR3014435B1 (fr) 2013-12-11 2016-10-21 Novasep Process Purification d'acides gras par un procede chromatographique
FR3014436B1 (fr) 2013-12-11 2016-10-21 Novasep Process Procede de purification chromatographique d'un acide gras
EP2883860B1 (fr) * 2013-12-11 2016-08-24 Novasep Process Procédé chromatographique de production d'acides gras polyinsaturés
JP6303017B2 (ja) 2014-01-07 2018-03-28 ノヴァセプ プロセスＮｏｖａｓｅｐ Ｐｒｏｃｅｓｓ 芳香族アミノ酸を精製する方法
CN103805338B (zh) * 2014-01-10 2016-12-07 陈汉卿 一种提高植物油中不饱和脂肪酸含量的短程蒸馏生产方法及短程蒸馏器
JP6664328B2 (ja) * 2014-09-17 2020-03-13 日本水産株式会社 エイコサペンタエン酸アルキルエステルを含有する組成物及びその製造方法
WO2017005235A1 (de) 2015-07-06 2017-01-12 K.D. Pharma Bexbach Gmbh Verfahren zur gewinnung von freier fettsäure und/oder fettsäureester
CN109563527A (zh) 2016-07-13 2019-04-02 赢创德固赛有限公司 将脂质与裂解的含脂质生物质分开的方法
RU2744913C2 (ru) 2016-12-27 2021-03-17 Эвоник Оперейшенс ГмбХ Способ выделения липидов из липидсодержащей биомассы
EP3470502A1 (en) 2017-10-13 2019-04-17 Evonik Degussa GmbH Method of separating lipids from a lysed lipids containing biomass
EP3527664A1 (en) 2018-02-15 2019-08-21 Evonik Degussa GmbH Method of isolating lipids from a lipids containing biomass
US11976253B2 (en) 2018-05-15 2024-05-07 Evonik Operations Gmbh Method of isolating lipids from a lysed lipids containing biomass by emulsion inversion
WO2019219443A1 (en) 2018-05-15 2019-11-21 Evonik Operations Gmbh Method of isolating lipids from a lipids containing biomass with aid of hydrophobic silica
CN110790337B (zh) * 2019-09-26 2020-09-08 江苏爱佳福如土壤修复有限公司 一种去除水体中重金属Cd的组合物及其制备方法与应用
KR102768762B1 (ko) 2022-09-08 2025-02-19 한국과학기술원 미세조류 내 epa의 고순도 연속 흡착분리 장치 및 이를 이용한 미세조류 내 epa의 고순도 연속 흡착분리 방법

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0633088A (ja) * 1992-07-21 1994-02-08 Nippon Suisan Kaisha Ltd 高純度ドコサヘキサエン酸またはそのエステルの 製造方法
JPH10152693A (ja) * 1996-11-22 1998-06-09 Osaka City ω−３系高度不飽和脂肪酸エステルの精製方法
JPH10310556A (ja) * 1997-05-12 1998-11-24 Y M Shii:Kk 微生物由来の多価不飽和脂肪酸エステルの分離精製方法
WO2000073254A1 (en) * 1999-05-31 2000-12-07 Jfs Envirohealth Ltd. Concentration and purification of polyunsaturated fatty acid esters by distillation-enzymatic transesterification coupling

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3400466B2 (ja) * 1991-10-28 2003-04-28 日本水産株式会社 高純度エイコサペンタエン酸またはそのエステルの製造方法
DK0697034T3 (da) * 1993-04-29 1997-07-14 Norsk Hydro As Fremgangsmåder til kromatografisk fraktionering af fedtsyrer og deres derivater
NO312973B1 (no) * 1999-02-17 2002-07-22 Norsk Hydro As Lipase-katalysert forestring av marine oljer
NO319194B1 (no) * 2002-11-14 2005-06-27 Pronova Biocare As Lipase-katalysert forestringsfremgangsmate av marine oljer

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0633088A (ja) * 1992-07-21 1994-02-08 Nippon Suisan Kaisha Ltd 高純度ドコサヘキサエン酸またはそのエステルの 製造方法
JPH10152693A (ja) * 1996-11-22 1998-06-09 Osaka City ω−３系高度不飽和脂肪酸エステルの精製方法
JPH10310556A (ja) * 1997-05-12 1998-11-24 Y M Shii:Kk 微生物由来の多価不飽和脂肪酸エステルの分離精製方法
WO2000073254A1 (en) * 1999-05-31 2000-12-07 Jfs Envirohealth Ltd. Concentration and purification of polyunsaturated fatty acid esters by distillation-enzymatic transesterification coupling

Also Published As

Publication number Publication date
WO2009154369A2 (ko) 2009-12-23
KR20090132048A (ko) 2009-12-30
US20110091947A1 (en) 2011-04-21
KR101357298B1 (ko) 2014-01-28

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