RU2686448C1 - Method for preparing a biologically active additive for animals and a biologically active additive for animals based thereon - Google Patents

Abstract

FIELD: veterinary medicine.SUBSTANCE: invention refers to veterinary science, particularly to methods for preparing a biologically active additive (BAD) and a product-based additive for animals. BAD preparation method involves collection of 9–10-day larvae of drones, storage until use with further homogenisation. Distilled water mixed with hydrochloric acid solution is additionally added to the homogenate and heated to temperature of 37–38 °C. Then, while stirring with a stirrer, renin is added to obtain 3 % solution and then, after complete dissolution, the preparation pancreatin is added, followed by stirring at temperature of 37–38 °C for 25–35 minutes. BAD is characterized by the following ratio of initial components, wt%: homogenate of drone larvae – 9.5–10.5, hydrochloric acid – 0.9–1.1, renin – 2.8–3.2, pancreatin – 0.45–0.55 and distilled water – the rest.EFFECT: invention allows increasing overall and specific body resistance and reproductive function of animals.2 cl, 3 tbl, 5 ex

Description

The technical field to which the invention relates.

The invention relates to veterinary medicine, in particular, to a method of preparing a dietary supplement for animals and a dietary supplement for animals based on it from natural components: drones larvae and enzyme preparations, and can be used to increase the overall and specific resistance of the organism and the reproductive function of animals .

The level of technology

There is a known method of preparing a preparation for stimulating the human body from drona larvae, including collecting drona larvae, homogenizing them with the addition of a stabilizing medium (see “Harvesting drona larvae” by V.I. Lebedev, MALogovich, Institute of Beekeeping, g. “Beekeeping” , No. 3, 2003, pp. 52-54).

The disadvantage of this method is the native (preserved state) state of the larvae.

A known method of preparing a preparation for stimulating the animals' organism from dronnage larvae, including collecting drona larvae, homogenizing them with the addition of a stabilizing medium from 5% glucose solution in a ratio of 1:10 with the addition of 5% chloroform solution or 2% salicylic acid with subsequent sublimation (see US Pat. of the Russian Federation No. 2258522, Cl. A61K 35/64, A61P 37/04, published. 08/20/2005).

The disadvantage of this method is that the drug obtained by this method is administered intramuscularly, causing a local reaction of the body.

The closest in technical essence and the achieved positive effect and adopted by the authors for the prototype is a method of preparing a lyophilized (dried) dietary supplement for animals and products based on it, including the collection of 9-10 days drona larvae, their freezing followed by homogenization and the addition of purified and homogenized pumpkin and distilled water, followed by lyophilization. Homogenization is carried out to a particle size of 0.5-1.0 mm (see US Pat. Of the Russian Federation No. 2435433 of the class A23K 1/00, publ. 10.12.2011).

The disadvantage of this method is the native state of the larvae, high energy consumption for lyophilization and the duration of the cooking process.

DISCLOSURE OF INVENTION

The task of the invention is to develop a method of preparation of biologically active additives (BAA) for animals and biologically active additives for animals based on it, which has a high stimulating effect, manifested in increasing the overall and specific resistance and reproductive function of animals, eliminating allergic reactions and cheaper to manufacture .

The technical result, which can be obtained using the present invention, is to increase the overall and specific resistance, the reproductive function of animals, the exception of allergic reactions, cheaper production.

The technical result is achieved by using the method of preparing a dietary supplement for animals, including collecting 9-10 days dwarf larvae, storing until use and subsequent homogenization, additionally add distilled water mixed with hydrochloric acid solution to the homogenate and heat to 37- 38 ° С, then, with stirring, renin is added with a mixer and then, after complete dissolution, the drug Pancreatin is added, followed by stirring with a mixer at a temperature of 37-38 ° С during s 25-35 minutes, with the following ratio of the starting components, in mass%:

drones larvae homogenate 9.5-10.5 hydrochloric acid 0.9-1.1 renin 2.8-3.2 pancreatin 0.45-0.55 distilled water rest

The technical result is achieved using a dietary supplement for animals, characterized by the fact that it is obtained by the above method and includes a domon larvae homogenate, hydrochloric acid, renin, pancreatin and distilled water in the following ratio of initial components, in mass%:

drones larvae homogenate 9.5-10.5 hydrochloric acid 0.9-1.1 renin 2.8-3.2 pancreatin 0.45-0.55 distilled water rest

Thus, in order to achieve a technical result, 9-10 day dwarf larvae are harvested, stored until used, followed by homogenization to a particle size of 0.5-1.0 mm, and additionally distilled water mixed with hydrochloric acid solution is added to the homogenate. heated to a temperature of 37-38 ° C, then with stirring with a mixer, add renin in masses. %: 2.8-3.2 to obtain a 3% solution and then after complete dissolution, the drug Pancreatin is added, followed by stirring with a stirrer at 37-38 ° C for 25-35 minutes, in the following ratio of initial components, in mass%:

drones larvae homogenate 9.5-10.5 hydrochloric acid 0.9-1.1 renin 2.8-3.2 pancreatin 0.45-0.55 distilled water rest

The finished solution is autoclaved at a temperature of 120 ° C and a pressure of 2 atmospheres.

Thus, the invention allows to increase the overall and specific resistance of animals, their weight, reproductive function, to eliminate allergic reactions, to reduce the cost of production.

The essence of the method of preparation of biologically active additives for animals and biologically active additives for animals based on it is as follows.

In order to obtain 9-10 days old drones' larvae during the rapid development of bee colonies, for example, 2 frames for each drone brood are placed in each hive and they are sown. At 9-10 days after the frames with the brood of drones are sown, they are sealed and taken from the hive. From the cells with the drone brood cut convex lids with a special knife or fork for printing combs. In a container that fits the size of the frame in size, the larvae of the drones are knocked out together with the royal jelly contained in the cells and collected in containers suitable for the volume. Before use, it is homogenized in a blender for 1.5 minutes at a speed of 3,000 rpm, then distilled water is added to the homogenate, mixed with hydrochloric acid solution and heated to 37-38 ° C, then, with stirring, renin is added to the masses . %: 2.8-3.2 to obtain a 3% solution and then after complete dissolution, the drug Pancreatin is added, followed by stirring with a stirrer at 37-38 ° C for 25-35 minutes, in the following ratio of initial components, in mass%:

drones larvae homogenate 9.5-10.5 hydrochloric acid 0.9-1.1 renin 2.8-3.2 pancreatin 0.45-0.55 distilled water rest

After which the BAA is packaged in 100 ml sterile glass bottles and stored in a household refrigerator at a temperature of + 4 ° C until use.

Brief description of drawings and other materials

Table 1 provides a method for preparing a dietary supplement for animals and a dietary supplement for animals based on it, hematological parameters of horses before and after the use of dietary supplements.

In table 2, too, biochemical blood parameters of horses before and after the use of dietary supplements.

In table 3, too, the biochemical composition of the digested larvae of drones,%.

The implementation of the invention

Examples of a specific implementation of the method of preparation of biologically active additives for animals and biologically active additives based on it.

Example number 1. Drones larvae are specially grown to 9-10 days of age during the rapid development of bee colonies. Frames with drone brood are removed from families, convex lids are cut from the cells and the drones larvae are knocked out together with the royal jelly contained in the cells into a baking sheet, then they are collected in containers suitable for the volume, and stored until used, followed by homogenization to particle sizes of 0.5 -1.0 mm, while in the homogenate in the mass. %: in the amount of 9.0 additional distilled water is added - the rest, mixed with a solution of hydrochloric acid in the amount of 0.8 and heated to a temperature of 37-38 ° C, then with stirring with stirrer add renin in the amount of 2, 6 and further after complete dissolution add the drug Pancreatin in the amount of 0.4, followed by stirring with a stirrer at a temperature of 37-38 ° C for 40 minutes, in the following ratio of initial components, in mass%:

drones larvae 9.0 hydrochloric acid 0.8 renin 2.6 pancreatin 0.4 distilled water rest

Thus, with this ratio of the initial components, the drona larvae are poorly (partially) digested, since the preparation uses a low content of hydrochloric acid, pancreatin and renin, which led to partial digestion of the drona larvae.

Example number 2. Carried out analogously to example No. 1, but stirred with a magnetic stirrer at a temperature of 37-38 ° C for 35 minutes and use the following ratio of the starting components, in wt. %:

drones homogenate 9.5 hydrochloric acid 0.9 renin 2.8 pancreatin 0.45 distilled water rest

Thus, at this ratio of the starting components, the homogenized dwarf larvae are almost completely digested (about 90%), as in Example No. 2, when preparing dietary supplements, the concentration of hydrochloric acid, renin and pancreatin is used high for digesting the drones larvae, as a result of which digesting drones larvae by 90%.

Example number 3. Carried out analogously to example No. 1, but stirred with a magnetic stirrer at a temperature of 37-38 ° C for 30 minutes and use the following ratio of initial components, in wt. %:

drones homogenate 10.0 hydrochloric acid 1.0 renin 3.0 pancreatin 0.5 distilled water rest

Thus, at this ratio of the starting components, the homogenized dwarf larvae are completely digested, as in Example No. 3, when preparing dietary supplements, the concentration of hydrochloric acid, renin and pancreatin is used high for digesting the drones larvae, resulting in 100% observation of the droner larvae. Digestion goes well, with the exception of chitinous inclusions.

Example number 4. Carried out analogously to example No. 1, but stirred with a magnetic stirrer at a temperature of 37-38 ° C for 25 minutes and use the following ratio of initial components, in wt. %:

drones homogenate 10.5 hydrochloric acid 1.1 renin 3.2 pancreatin 0.55 distilled water rest

Thus, at this ratio of the initial components, the homogenized dwarf larvae are almost completely digested (about 90%), as in Example No. 3, when preparing dietary supplements, the concentration of hydrochloric acid, renin and pancreatin is used high for digesting the drones larvae, as a result of which digesting drones larvae by 90%.

Example number 5. Carried out analogously to example No. 1, but stirred with a magnetic stirrer at a temperature of 37-38 ° C for 20 minutes and use the following ratio of initial components, in wt. %:

drones larvae homogenate 11.0 hydrochloric acid 1.2 renin 3.4 pancreatin 0.6 distilled water rest

Thus, with this ratio of initial components, it turned out that this content of components in mass % is not appropriate, because high content of components leads to protein breakdown and low content of amino acids, and the most optimal were examples No. 2, No. 3 and No. 4, for example, the dietary supplement obtained by these methods, which is given to animals inside with feed, reduces the time spent digesting drona larvae by four times. Experimental testing on the use of dietary supplements was carried out on horses in a pilot farm of the Stavropol Agrarian University, while the hematological parameters of horses before and after the use of the dietary supplement are shown in table 1, the biochemical parameters of the blood of horses before and after the application of the dietary supplement are shown in table 2, and the biochemical composition of digested larvae drones ,% is given in table 3, the results, the data in the tables show the positive effect of dietary supplements on the horse's body, which confirms the achievement of the technical result.

The proposed invention in comparison with the prototype and other known technical solutions has the following advantages:

- BAA increases the general and specific resistance of the organism of animals and the reproductive function of animals;

- cheaper to manufacture;

- due to the use of hydrochloric acid, renin, pancreatin and distilled water, complete digestion of homogenized dronnage larvae occurs, which improves the digestibility of dietary supplements;

- reduced production time;

- does not require special high-tech equipment;

- does not have allergic effects;

- is introduced inside.

Claims (4)

1. The method of preparation of biologically active additives for animals, including the collection of 9-10-day dwarf larvae, storage until use, followed by homogenization, characterized in that the homogenate additionally make distilled water mixed with a solution of hydrochloric acid, and heated to a temperature of 37- 38 ° C, then, with stirring, renin is added with a mixer to obtain a 3% solution and then, after complete dissolution, the drug Pancreatin is added, followed by stirring with a mixer at a temperature of 37-38 ° C for 25-35 Inuit, starting with the following ratio of components, wt. %: drones larvae homogenate 9.5-10.5 hydrochloric acid 0.9-1.1 renin 2.8-3.2 pancreatin 0.45-0.55 distilled water rest 2. Biologically active additive for animals, characterized by the fact that it is obtained by the method according to p. 1 and includes the homogenate of the drones' larvae, hydrochloric acid, renin, pancreatin and distilled water in the following ratio of initial components, wt. %: drones larvae homogenate 9.5-10.5 hydrochloric acid 0.9-1.1 renin 2.8-3.2 pancreatin 0.45-0.55 distilled water rest