RU2533156C1 - Method of preparing biologically active additive for animals and agent based on it - Google Patents

Abstract

FIELD: veterinary medicine.

SUBSTANCE: method comprises collecting of 9-10 days old larvae of drones, their freezing, storage prior to use, followed by homogenization to a particle size of 0.5-1.0 mm. The wheat bran is added to the resulting homogenate, followed by mixing of the components. The bran is preliminary adjusted to a moisture content of 5-6% at a temperature of 110°C for 1.5-2 hours and cooled to the temperature of 20-25°C. At that mixing is carried out for 20-30 minutes, and the components are taken in a specific ratio. The resulting biologically active additive provides enhancement of general and specific resistance, reproductive function of animals while avoiding allergic reactions.

EFFECT: cheapening of the additive manufacturing.

2 cl, 4 tbl, 4 ex

Description

FIELD OF THE INVENTION

The invention relates to veterinary medicine, in particular to methods for preparing a biologically active additive and a tool based on it from natural components: drones and bran larvae, and can be used to increase the general and specific resistance of the organism and the reproductive function of animals.

State of the art

There is a method of preparing a drug for stimulating the human body from drones larvae, including collecting drones larvae, their homogenization with the addition of a stabilizing medium (see. "Harvesting drones larvae" VI Lebedev, MA Logovich, Research Institute of beekeeping, g. "Beekeeping ", No. 3, 2003, p. 52-54).

The disadvantage of this method is the short storage time of the drug.

There is a method of preparing a drug for stimulating the body of animals from drones larvae, including collecting drones larvae, homogenizing them with the addition of a stabilizing medium from a 5% glucose solution in a ratio of 1:10 with the addition of a 5% solution of chloroform or 2% salicylic acid with subsequent sublimation (see US Pat. RF No. 2258522, class A61K 35/64, A61P 37/04, publ. 08.20.2005).

The disadvantage of this method is that the drug obtained by this method is administered intramuscularly, causing a local reaction of the body.

The closest in technical essence and the achieved positive effect and adopted by the authors for the prototype is a method of preparing a lyophilized biologically active additive for animals and products based on it, including the collection of 9-10-day drone larvae, their freezing, followed by homogenization and the addition of peeled and homogenized pumpkin and distilled water, followed by lyophilization. Homogenization is carried out to a particle size of 0.5-1.0 mm (see US Pat. RF No. 2435433 class A23K 1/00, publ. 10.12.2011).

The disadvantage of this method is the high energy consumption for lyophilization and the duration of the cooking process.

Disclosure of invention

The objective of the invention is to develop a method for the preparation of a biologically active additive (BAA) from larvae of drones and wheat bran and a tool based on it that has a high stimulating effect, manifested in an increase in the general and specific resistance and reproductive function of animals, eliminating allergic reactions and cheaper to manufacture .

The technical result, which can be obtained using the present invention, is to increase the overall and specific resistance, reproductive function of females and males, the exclusion of allergic reactions, cheaper manufacturing.

The technical result is achieved using a method of preparing a biologically active additive for animals, including the collection of 9-10-day drones larvae, their freezing, storage until use, followed by homogenization to particle sizes of 0.5-1.0 mm, characterized in that the homogenate additionally, wheat bran is added, followed by mixing, which is previously brought to a moisture content of 5-6% at a temperature of 110 ° C for 1.5-2 hours and cooled to a temperature of 20-25 ° C, while mixing is carried out for 20-30 minutes in the ratio of comp nents in wt.%:

drone larvae homogenate 10.0 wheat bran 90.0

The technical result is achieved using a biologically active feed additive, including a homogenate of drones larvae and pre-dried wheat bran with a ratio of components in wt.%:

drone larvae homogenate 10.0 wheat bran 90.0

Wheat bran contains a large amount of dietary fiber and B vitamins (table 3), and they are used as a food supplement to normalize digestion.

Dried wheat bran is used to quickly absorb moisture from the homogenate of drones. In this case, the homogenate is not exposed to high temperatures, which contributes to better preservation of the active components contained in the drone mass. Due to the low humidity - not more than 15% - a long shelf life of the product is achieved without spoiling it.

Thus, the group of inventions allows to increase the general and specific resistance of animals, their weight, reproductive function, eliminate allergic reactions, and reduce the cost of manufacturing.

The essence of the method of preparation of biologically active additives and funds based on it is as follows.

To obtain 9-10-day drones larvae during the period of rapid development of bee colonies, 2 frames for drone brood are placed in each hive and their seeding is monitored. On the 9-10th day after the framework with the brood of drones is sealed, they are taken from the hive. Convex caps are cut from drone brood cells using a special knife or fork for printing honeycombs. Drones together with royal jelly contained in the cells are knocked out into dishes that are suitable in size to the size of the frame and collected in containers suitable for volume. Then, if necessary, it is frozen at a temperature of -18 ... -20 ° C and stored for use no more than six months. Before use, they are thawed at a temperature of 0 ... + 4 ° C and homogenized in a blender for 1 ... 1.5 min at a speed of 3 thousand rpm.

Wheat bran is scattered on baking sheets and dried in an oven at a temperature of + 110 ° C for 1.5-2 hours to a moisture content of not more than 5-6%. Then cool to room temperature (+ 20 ... 25 ° C) and mixed with a homogenate of drones larvae in the following ratio, mass. %:

drone larvae homogenate 10.0 wheat bran 90.0

using "Granulator-30" for 20-30 minutes until a homogeneous mass with a moisture content of 14-15% is obtained. Then packaged in paper bags, which are stored at room temperature until use.

A brief description of the drawings and other materials.

Table 1 gives a method of preparing a biologically active additive for animals and a tool based on it, hematological parameters of horses before and after the use of dietary supplements

In table 2 - the same, Biochemical blood parameters of horses before and after the use of dietary supplements.

Table 3 shows the same composition of wheat bran.

Table 4 shows the same biochemical composition of the raw material from drones larvae,%.

The implementation of the invention

Examples of specific performance of the method of preparation of biologically active additives for animals and funds based on it.

Example No. 1

Drones larvae are specially grown up to 9-10 days of age during the rapid development of bee colonies. The frames with the drone brood are removed from the families, the convex caps are cut off from the cells and the larvae of the drones together with the royal jelly contained in the cells are knocked out into the pan, then they are collected in containers of suitable volume, sealed and frozen at a temperature of -18 ... -20 ° C and store until use 2-3 months. Before use, they are thawed at a temperature of 0 ... + 4 ° C and homogenized in a blender for 1 ... 1.5 min at a speed of 3 thousand rpm.

The prepared homogenate of drone larvae is mixed with wheat bran with a moisture content of about 15% in the following ratio, wt.%:

drone larvae homogenate 10.0 wheat bran 90.0

using "Granulator-30" for 20-30 minutes until a homogeneous mass with a moisture content of 20-21% is obtained. The resulting mixture in organoleptic properties is indistinguishable from wheat bran. When used, it gives a high expected effect. However, the additive does not withstand the shelf life of 1 year.

Example No. 2

Carried out analogously to example 1, but the bran is subjected to preliminary drying in a thermostat. To do this, they are scattered with a layer of 3-3.5 cm on a baking sheet of the thermostat and left at a temperature of 110 ° C until a moisture content of 4-5% is reached for 3 hours. Then cool to room temperature (+ 20 ... 25 ° C) and mixed with a homogenate of drones larvae in the following ratio, wt.%:

drone larvae homogenate 20,0 wheat bran 80.0

using "Granulator-30" for 20-30 minutes until a homogeneous mass with a moisture content of 20-21% is obtained. Then packaged in paper bags, which are stored at room temperature until use.

The resulting preparation does not differ in appearance and smell from wheat bran. However, it does not withstand the specified shelf life of 1 year. When used, it gives a high effect of increasing resistance (see tables 1, 2).

Example No. 3

Carried out analogously to example 2, but the same number of bran is subjected to preliminary drying in an thermostat on two baking sheets with a layer of 1-1.5 cm at a temperature of + 110 ° C for 1.5-2 hours to a moisture content of not more than 5-6%. Then they are cooled to room temperature (+ 20 ... 25 ° C) and mixed with a homogenate of drone larvae in the following ratio, mass. %:

drone larvae homogenate 10.0 wheat bran 90.0

using "Granulator-30" for 20-30 minutes until a homogeneous mass with a moisture content of 14-15% is obtained. Then packaged in paper bags, which are stored at room temperature until use.

The resulting preparation does not differ in appearance and smell from wheat bran. It maintains the established shelf life of 1 year. When used, it gives a high effect of increasing resistance (see tables 1, 2).

Example No. 4

Drying bran is carried out analogously to example 3, then they are cooled to room temperature (+ 20 ... 25 ° C) and mixed with a homogenate of drones larvae in the following ratio, mass. %:

drone larvae homogenate 5,0 wheat bran 95.0

using "Granulator-30" for 20-30 minutes until a homogeneous mass with a moisture content of 10-12% is obtained.

The resulting preparation does not differ in appearance and smell from wheat bran. It maintains the established shelf life of 1 year. However, when used to achieve a positive effect, it is necessary to increase the dose of the drug, which leads to a violation of the dietary structure in animals due to an increase in its fiber and phosphorus content.

Thus, the most optimal examples were No. 2 and No. 3. Supplements obtained in this way are given to animals inside with food. However, when using example No. 3, the energy consumption for drying is halved and the shelf life is increased to 1 year.

The invention in comparison with the prototype and other known technical solutions has the following advantages:

- the drug increases the general and specific resistance of the animal organism;

- cheaper to manufacture;

- drying is carried out by mixing with air-dry bran at room temperature, which avoids the heating of the larval mass and prevents loss of activity of the protein substances contained in it;

- reduced manufacturing time;

- special high-tech equipment is not required;

- does not have allergic effects;

- is introduced inside;

- has a shelf life of 1 year.

Table 1 Hematological parameters of horses before and after the use of dietary supplements Indicator Carrier Blood Test Results before applying dietary supplements after applying dietary supplements White blood cells, 10 ⁹ / L 7.8 ± 1.15 9.7 ± 1.34 Red blood cells, 10 ¹² / L 7.8 ± 0.37 7.9 ± 0.42 Hemoglobin, g / l 129.4 ± 10.24 122.0 ± 7.97 Hematocrit% 38.0 ± 3.00 37 ± 2,00 The average volume of the red blood cell, microns ³ 47.9 ± 1.70 46.6 ± 1.42 The average concentration of Hb in the red blood cell,% 16.4 ± 0.62 15.4 ± 0.57 The average content of Hb in red blood cells, PG 343.8 ± 6.76 331.0 ± 3.18 Platelets, 10 ⁹ / L 127.8 ± 4.84 158.8 ± 10.94 ESR, mm / h 32.0 ± 12.23 27.6 ± 9.64 Eosinophils,% 1.6 ± 1.36 3.4 ± 0.87 Band neutrophils,% 1.4 ± 0.51 2.8 ± 0.97 Segmented neutrophils,% 45.2 ± 4.68 52.0 ± 2.39 Lymphocytes,% 45.4 ± 4.25 38.6 ± 1.78 Monocytes,% 6.6 ± 1.21 3.8 ± 0.97

table 2 Biochemical blood parameters of horses before and after the use of dietary supplements Indicator Carrier Blood Test Results before applying dietary supplements after applying dietary supplements Bilirubin total, μmol / l 34.8 ± 12.24 34.5 ± 10.06 AsAT, units / l 273.6 ± 18.54 305.3 ± 14.01 AlAT, units / l 18.1 ± 1.20 17.0 ± 0.79 Glucose, mmol / L 4.6 ± 1.05 5.3 ± 0.16 Urea, mmol / L 5.8 ± 1.02 6.4 ± 0.54 Creatinine, μmol / L 132.6 ± 11.76 104.7 ± 5.86 Total protein, g / l 62.4 ± 3.06 63.9 ± 1.45 Amylase, units / l 11.8 ± 5.15 15.5 ± 3.66 Thymol test, units 2.5 ± 0.63 2.9 ± 0.56

Table 3 Wheat bran composition Indicator Content in 100 g of product The nutritional value 216 kcal The energy value 904 kj Water 9.9 g Squirrels 15.6 g Fats 4.3 g Carbohydrates 64.5 g - disaccharides 0.41 g - alimentary fiber 42.8 g Thiamine (B1) 0.5 mg Riboflavin (B2) 0.6 mg Niacin (B3) 13.6 mg Pantothenic Acid (B5) 2.2 mg Pyridoxine (B6) 1.3 mg Tocopherol (Vit. E) 1.5 mg Vitamin K 1.9 mcg Calcium 73 mg Iron 11 mg Magnesium 611 mg Phosphorus 1013 mg Potassium 1182 mg Zinc 7 mg

Table 4 Biochemical composition of raw materials from drones larvae,% Indicator Drones Larvae, Raw Moisture 78.9 Crude protein 7.78 Crude fat 4.43 Bev 8.15 Amino acids Aspartic acid 0.682 Threonine 0.291 Series 0,308 Glutamic acid 1,076 Proline 0.532 Glycine 0.323 Alanine 0.392 Valine 0.395 Isoleucine 0.383 Leucine 0.592 Tyrosine 0.369 Phenylalanine 0.322 Histidine 0.229 Lysine 0.266 Arginine 0.393 Cystine 0.135 Methionine 0.255

Claims (2)

1. A method of preparing a biologically active additive for animals, including the collection of 9-10-day drones larvae, their freezing, storage until use, followed by homogenization to particle sizes of 0.5-1.0 mm, characterized in that wheat is additionally added to the homogenate bran, followed by mixing, which is previously brought to a moisture content of 5-6% at a temperature of 110 ° C for 1.5-2 hours and cooled to a temperature of 20-25 ° C, while mixing is carried out for 20-30 minutes in the ratio of components in wt.%:
drone larvae homogenate 10.0 wheat bran 90.0 2. A biologically active feed additive for animals, obtained by the method according to claim 1, characterized in that it further comprises pre-dried wheat bran in the following ratio of components in wt.%:
drone larvae homogenate 10.0 wheat bran 90.0