LU505734B1 - Preparation method of honeycomb extract and its application in broiler growth - Google Patents
Preparation method of honeycomb extract and its application in broiler growth Download PDFInfo
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- LU505734B1 LU505734B1 LU505734A LU505734A LU505734B1 LU 505734 B1 LU505734 B1 LU 505734B1 LU 505734 A LU505734 A LU 505734A LU 505734 A LU505734 A LU 505734A LU 505734 B1 LU505734 B1 LU 505734B1
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- honeycomb
- extract
- broilers
- honeycomb extract
- residue
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K59/00—Honey collection
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K40/00—Shaping or working-up of animal feeding-stuffs
- A23K40/10—Shaping or working-up of animal feeding-stuffs by agglomeration; by granulation, e.g. making powders
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K40/00—Shaping or working-up of animal feeding-stuffs
- A23K40/30—Shaping or working-up of animal feeding-stuffs by encapsulating; by coating
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
- A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
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- Life Sciences & Earth Sciences (AREA)
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- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Environmental Sciences (AREA)
- Birds (AREA)
- Biodiversity & Conservation Biology (AREA)
- Health & Medical Sciences (AREA)
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- Biotechnology (AREA)
- Molecular Biology (AREA)
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- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses a preparation method of a honeycomb extract, which is prepared according to the following method: mixing honeycomb residue with alcohol, soaking overnight, heating to boil, cooling, filtering, concentrating to obtain a honeycomb extract, granulating the honeycomb extract to obtain a coated honeycomb extract, also discloses the application of the honeycomb extract in the growth performance of broilers, improving the immune function of broilers, improving the antioxidant function of broilers, improving the intestinal health of broilers. The invention has the beneficial effects that the extraction process of honeycomb residue is optimized, the honeycomb residue extract is used as a broiler feeding additive instead of antibiotics, the honeycomb residue extract can promote the growth performance, immune, antioxidant functions and intestinal health of broilers, further exert the utilization value of honeycomb residue, improve food safety and quality, and promote the green, healthy and sustainable development of livestock and poultry breeding.
Description
DESCRIPTION LU505734
PREPARATION METHOD OF HONEYCOMB EXTRACT AND ITS APPLICATION
IN BROILER GROWTH
The invention relates to a preparation method of honeycomb extract and its application in broiler growth, belonging to the technical field of preparation and application of honeycomb extract.
With the rapid development of intensive and large-scale chicken industry, broilers are facing many outstanding problems, such as weak disease resistance and decreased meat quality. Feeding antibiotics can effectively enhance animal immune function, improve animal production performance and improve animal health, and make outstanding contributions to modern chicken industry, but the negative effects such as drug resistance and drug residue seriously threaten the safety of livestock and poultry products and human health. Today, with the increasing attention to the food safety of livestock and poultry, it is one of the research hotspots to actively find feed additives that are more advantageous than antibiotics, green, healthy and pollution-free to improve the growth performance, immune function and meat quality of broilers, so as to meet the large demand for high-quality broilers in the market. Honeycomb is a nest with wax structure on both sides, which is built by bee secretions and collections. After generations of bees, there are many bee products left in it, so it contains many ingredients of bee products such as honey, beeswax, bee pollen, royal jelly, propolis, etc., and has the effects of improving immunity, anti-inflammatory and anti-oxidation. As a dietetic product and medicine, it is used by human beings for rhinitis, rheumatoid arthritis, mastitis, dermatosis, liver disease, hypertension, etc.
China is a big bee-producing country, with a large scale of bee colonies, and tH&J505734 by-products such as beehives need to be replaced and eliminated regularly, with a huge output. Honeycomb is mostly used for the clinical compatibility of human medicine and the extraction of beeswax, and the extraction process is mostly decocting or leaching with water, but beeswax only accounts for 25% in honeycomb, and contains a lot of water-insoluble components, which causes a lot of waste of other effective components in honeycomb residue. Therefore, it is of great research value and significance to further develop and utilize honeycomb residue and explore its application as an antibiotic substitute product in livestock and poultry production.
The technical problem to be solved by the invention is to provide a preparation method of honeycomb extract and its application in broiler growth.
The invention is realized by the following scheme: a preparation method of a honeycomb extract is prepared by the following method: mixing honeycomb residue with alcohol, soaking overnight, heating to boil, cooling, filtering and concentrating to obtain a honeycomb extract, and granulating the honeycomb extract to obtain a coated honeycomb extract.
The material-liquid ratio of the honeycomb residue to the alcohol is 1:2.
The heating temperature is 60-100°C and the boiling time is 30-90min.
Honeycomb extract prepared according to the above method.
Application of honeycomb extract in broiler growth.
Application of honeycomb extract in growth performance of broilers.
Application of honeycomb extract in improving immune function of broilers.
Application of honeycomb extract in improving antioxidant function of broilers.
Application of honeycomb extract in improving intestinal health of broilers.
The invention has the beneficial effects that the extraction process of honeycomt/505734 residue is optimized, and the honeycomb residue extract is used as a broiler feeding additive instead of antibiotics, and the honeycomb residue extract can promote the growth performance, immune and antioxidant functions and intestinal health of broilers, further exert the utilization value of honeycomb residue, improve food safety and quality, and promote the green, healthy and sustainable development of livestock and poultry breeding.
The present invention will be further described below, but that scope of protection of the present invention is not limited to the content.
For the sake of clarity, all the features of the actual embodiment are not described, and in the following description, well-known functions and structures are not described in detail, because they will confuse the present invention with unnecessary details. It should be considered that in the development of any actual embodiment, a lot of implementation details must be made to achieve the specific goals of developers, such as changing from one embodiment to another according to the restrictions of related systems or related businesses. In addition, it should be considered that such development work may be complicated and time-consuming, but for the development of any actual embodiment,
Example 1: A preparation method of honeycomb extract, which is prepared according to the following method: mixing honeycomb residue and alcohol according to the ratio of 1: 2, soaking overnight, heating at 60°C for 30min, cooling, filtering and concentrating to obtain honeycomb extract, and granulating the honeycomb extract to obtain coated honeycomb extract.
Honeycomb extract prepared according to the above method.
Application of honeycomb extract in broiler growth.
Application of honeycomb extract in growth performance of broilers.
Application of honeycomb extract in improving immune function of broilers.
Application of honeycomb extract in improving antioxidant function of broilers. LU505734
Application of honeycomb extract in improving intestinal health of broilers.
Example 2: A preparation method of honeycomb extract, which is prepared according to the following method: mixing honeycomb residue and alcohol at a ratio of 1: 2, soaking overnight, heating at 100°C for 90min, cooling, filtering and concentrating to obtain honeycomb extract, and granulating the honeycomb extract to obtain coated honeycomb extract.
Honeycomb extract prepared according to the above method.
Application of honeycomb extract in broiler growth.
Application of honeycomb extract in growth performance of broilers.
Application of honeycomb extract in improving immune function of broilers.
Application of honeycomb extract in improving antioxidant function of broilers.
Application of honeycomb extract in improving intestinal health of broilers.
Example 3: A preparation method of honeycomb extract, which is prepared according to the following method: mixing honeycomb residue and alcohol at a ratio of 1: 2, soaking overnight, heating at 80°C for 60min, cooling, filtering and concentrating to obtain honeycomb extract, and granulating the honeycomb extract to obtain coated honeycomb extract.
Honeycomb extract prepared according to the above method.
Application of honeycomb extract in broiler growth.
Application of honeycomb extract in growth performance of broilers.
Application of honeycomb extract in improving immune function of broilers.
Application of honeycomb extract in improving antioxidant function of broilers.
Application of honeycomb extract in improving intestinal health of broilers.
The functions, effects and safety of the honeycomb extract in the growth performance, immune function improvement, antioxidant function improvement and intestinal health improvement of broilers were verified by experiments.
1 materials and methods LU505734 1.1 test materials
Mix the honeycomb residue with alcohol 1:2, soak overnight, boil at 100°C for 60min, cool, filter and concentrate to obtain honeycomb extract, and granulate the extract to obtain coated honeycomb extract, in which the content of total flavonoids is 0.29g/100g and the content of polysaccharide is about 0.16 g/100 g. 1.2 experimental design and feeding management 480 1-day-old female dwarf yellow broilers in the same batch were randomly divided into 5 groups with 6 replicates in each group and 16 broilers in each replicate. The blank control group was fed with basic diet, and the antibiotic control group was fed with basic diet supplemented with antibiotics (chlortetracycline for poultry 150ppm). The experimental group 1, experimental group 2 and experimental group 3 were fed with 1%, 1.5% and 2% honeycomb extract respectively. The pre-feeding period is 20 days, and the trial period is divided into two stages, the first stage is 21-50 days, and the second stage is 51-80 days. The experiment was carried out in the experimental chicken farm of
Institute of Animal Husbandry and Veterinary Medicine, Jiangxi Academy of Agricultural
Sciences. The experimental chickens were reared in rows on the Internet, and they were free to eat and drink water during the experiment. Immunization and disinfection are carried out according to routine procedures.
The dietary composition and nutritional level at different stages of the experiment are shown in Table 1.
Table 1 Composition and nutrient level of basal diets(air-drybasis) % LU505734
Content
Items 21-50 51-80 d d
Ingredients
Corn 64.00 65.00
Soybean oil 3.00 4.00
Soybean meal 26.00 26.00
Fish meal 2.00 -
Premix 5.00 5.00
Total 100.0 100.00 0
Nutrient level
Metabolizable energy 12.66 13.43
Crude protein 18.38 17.15
Calcium (Ca) 0.90 0.70
Total phosphorus 0.60 0.45
Lysine 1.17 1.07
Methionine 0.45 0.42
Cysteine 0.31 0.30
Methionine+cysteine 0.76 0.72
Tryptophan 0.21 0.19
Threonine 0.70 0.64
Arginine 1.21 1.13
2 Determination indicators and methods LU505734 2.1 Detection of Flavonoids and Polysaccharides in Honeycomb Extract
According to GB 5009. 8-2016 and GB/T 20574-2006, the contents of polysaccharides and flavonoids in honeycomb extracts were detected. 2.1 Determination of growth performance index
During the experiment, the feed rate, residual feed rate, initial weight and final weight of experimental chickens were recorded in units of repetitions, and the average daily gain (ADG), average daily feed intake (ADFI) and feed-to-weight ratio (F/G) of experimental chickens were calculated. 2.2 Blood sample collection and index determination
At the end of the experiment, the chickens were fasted for 12 hours, and T-AOC chickens with similar weight were randomly selected from each repetition. After weighing, 5mL of blood was taken from the vein under the wing, centrifuged at 3500r/min for 10min, and stored at -20°C for the determination of serum immunoglobulin A(lgA), immunoglobulin G(IgG), immunoglobulin M(IgM), malondialdehyde (MDA) and glutathione peroxide (GSH-Px). The test kit was purchased from Nanjing Jiancheng
Institute of Bioengineering. 2.3 Determination of carcass performance index
Slaughtering the neck of the chicken after blood collection, unhairing, removing trachea, esophagus, bursa, intestine, spleen, pancreas, gallbladder and reproductive organs, and weighing the chicken horny membrane, and calculating the semi-clean rate;
The heart, liver, glandular stomach, muscular stomach, lung, abdominal fat and head and feet were removed on the basis of semi-clean bore, and then weighed again, and the total clean bore rate was calculated (when the head was removed, the skin was cut at the junction of the first cervical vertebra and the head; Cut along the tarsal joint when removing the foot); Quickly peel off the left chest muscle, leg muscle and abdominal fat, weigh and calculate the indexes such as chest muscle rate, leg muscle rate and abdominal fat rate. [Refer to Terminology and Statistical Methods of Poultry Production
Performance (NY/T823-2004)].
Slaughter weight: the weight after bleeding to remove feathers, cuticle of feet, td&/505734 shell and beak shell, and it should be drained by wet feather plucking method before weighing.
Slaughtering rate (%) = slaughter weight/pre-slaughter weight x 100%;
Semi-clean bore rate (%) = semi-clean bore weight/pre-slaughter weight x 100%;
Total clean bore rate (%) = total clean bore weight/pre-slaughter weight x 100%;
Pectoral muscle rate (%) = bilateral pectoral muscle weight/total clean bore weight x 100%;
Leg muscle rate (%) = net muscle weight of both legs/total net bore weight x 100%;
Abdominal fat rate (%) = abdominal fat weight/(total clean bore weight+abdominal fat weight) x 100%. 2.4 Determination of immune organ index
After slaughter, the immune organs such as spleen, bursa of fabricius and thymus were separated, and the surrounding excess fat was removed. The blood was drained with filter paper and weighed. According to the formula: immune organ index = immune organ weight/live weight before slaughter x 100%, the immune organ index was calculated. 2.5 intestinal microbial count
After the experimental ducks were killed by cutting the jugular vein, they were immediately laparotomy. One side of the cecum was ligated with sterilized cotton thread at the ileocecal node, and the outer end of the cecum was cut off. The ligated place was sterilized with alcohol cotton balls, put in sterilized self-sealing bags, put in an ice box at 4°C for preservation, and brought back to the laboratory. About 0.5g of chyme was collected on a sterile operating table, and mixed thoroughly in 10mL of normal saline, diluted with 10 times gradient, and 100 samples with corresponding dilution gradient were absorbed by different strains. Inoculated to the corresponding nutrient agar medium plate, the total bacteria, Escherichia coli, lactic acid bacteria and yeast were cultured in NA medium, EMB medium, MRS medium and YSD medium respectively.
After coating evenly with a coater, the total bacteria and Escherichia coli wet&/505734 cultured in a constant temperature incubator at 37°C for 24 hours, yeast in a constant temperature incubator at 30°C for 36 hours and lactic acid bacteria in an anaerobic incubator at 37°C for 36 hours. Bacillus selected suitable dilution gradient, maintained in water bath at 80°C for 10min, absorbed 100uL, inoculated on NA medium, and cultured at 37°C for 36 hours. Counting after the end of culture, 2 replicates per sample.
Number of heavy colonies per gram of intestinal contents = LG [(average number of coloniesx dilution multiplex 10ml/0.1ml)/content mass]. 3 Statistical analysis of results
The experimental data were analyzed by spss19.0 software, compared by one-way
ANOVA, and compared by Duncan's multiple methods, with P < 0.05 as the criterion for judging the significance of the difference, with P < 0.05 representing the significant difference and P > 0.05 representing the insignificant difference. The test results are expressed by Mean+SD. 4 Test results 4.1 Effect of Honeycomb Extract on Growth Performance of Broilers
From the following table, it can be seen that adding different proportions of honeycomb extract in broiler diet has significant effects on the growth performance of broilers at different stages. In the early stage of the experiment, compared with the non-antibiotic control group, adding 2.0% honeycomb extract to the diet can significantly increase the average daily gain (P < 0.05), and the feed-to-meat ratio decreased by 5.7%, with a significant difference (P < 0.05). Compared with the anti-biotic control group, there is no significant difference in the average daily feed intake, average daily gain and feed-to-weight ratio of the experimental broilers with different proportions of honeycomb extract (P > 0.05). In the later stage of the experiment, compared with the control group without resistance, adding more than 1.0% of honeycomb extract can significantly increase the average daily feed intake and average daily gain of experimental broilers (P < 0.05), and the feed-to-weight ratio decreased by 14.4%, with a significant difference (P < 0.05). Compared with the antibiotic control group, there was no significant difference in average daily feed intake, average daily gain and feed-to-weight ratio between the experimental broilers added with more than 1.0% honeycomb extract (P > 0.05). DuringJ505734 the whole experimental period, compared with the control group without resistance, adding 1.5% honeycomb extract in the diet can significantly increase the average daily feed intake of broilers (P < 0.05), adding more than 1.0% honeycomb extract in the diet can significantly increase the average daily weight gain of broilers (P < 0.05), and adding 1.0%, 1.5% and 2.0% honeycomb extract to the feed can reduce the feed-to-meat ratio by 5.5%, 5.5% and 8.1% respectively, and the statistical difference is significant (P < 0.05).
Table 2 Effect of honeycomb extract on growth performance of broilers
Honeycomb extract, % p-value
Antibiot — — —m—m——— —m————e—— —m—daJzaJabz m —z —
Items ic 0.0(bla Stand rd error ANOV Line Seconda nk 0.1 015 0.2 group A ar ry control) 21-50 d
ADFl,g 50.90 50.47 50.52 0.61 0.880 50.60 50.57 0.076 0.415 /d 4
ADG,g/ 20.23 19.09 20.04 0.00 0.022 19.92 2032 0.154 0.065 d 7
FIG 2.52 2.65 2.53 0.01 0.038 254 249 0.020 0.116 2 51-80 d
ADFl,g 79.122 7252° 7877 7825 77.46 0.00 <0.001 0.589 0.001 /d a a a 5
Honeycomb extract, % p-value LUS05734
Antibiot ——""
Items ic 0.0(bla Stand rd error ANOV Line Seconda nk 0.1 015 02 group A ar ry control)
ADG,g/ 20.599 16.56’ 19.88 < <0.001 20.28 20.51 d a 0.389 0.002 0.00 a a 1
FIG 3.86° 4.432 3.98 0.00 0.003 3.86° 3.79» 0.065 0.013 1 21-80 d
ADFl,g 64.918 61.91° 63.38 64.58 63.40 0.16 0.034 0.302 0.010 /d ab a ab 8
ADG,g/ 20.368 18.00° 19.45 < <0.001 1980 19.98 <0.00 d b 0.175 0.00 ab ab 1 1
F/G 3.19 3.452 3.26° < <0.001 <0.00 3.26° 3.17° 0.023 0.00 1 1
Note: The same or no letters in peer data indicate no significant difference (P > 0.05), while different letters indicate significant difference (P < 0.05). The following table is the same. 4.2 Effect of Honeycomb Extract on Slaughtering Performance of Broilers
From the following table, it can be seen that adding different proportions of honeycomb extract to broiler diet has certain influence on broiler slaughter performance.
Adding 1.0%, 1.5% and 2.0% honeycomb extract to the diet increased the slaughter rate of broilers by 1.4%, 1.2% and 1.7% respectively, and the difference between the 2.0%4J505734 honeycomb extract added group and the control group was significant (P < 0.05). Adding 1.0%, 1.5% and 2.0% honeycomb extract to the diet increased the semi-clean rate of broilers by 1.7%, 2.3% and 2.0%, respectively, and the differences were significant compared with the control group (P < 0.05). Diets supplemented with 1.0%, 1.5% and 2.0% of honeycomb extract increased the total clean-bore rate of broilers by 1.1%, 2.3% and 1.4% respectively, and the difference between the 1.5% honeycomb extract added group and the control group was significant (P < 0.05). Compared with the antibiotic control group, there was no significant difference in slaughter performance between the diet supplemented with more than 1.0% honeycomb extract (P < 0.05). There was no significant difference in breast muscle rate, leg muscle rate and abdominal fat rate among the experimental groups (P > 0.05).
Table 3 Effect of dietary Honeycomb extract on slaughter performanceof broilers LU505734
Honeycomb
Antibi extract. % Stang P-value
Items otic —————— ard —— group Où 01 015 02 error ANO Line Second
VA ar ary 88.572 880 893 891 896 0.01 0.030
Slaughter rate, % 0.181 0.048 b gb 3a gab 1a 1
Semi-clean bore 81.662 806 820 825 822 0.00 0.008 0.193 0.018 rate, % b 6b 32 0? 98 4
Total clean bore 65.9 66.7 675 66.8 0.06 0.115 66.62 0.202 0.197 rate, % 8 3 2 8 8
Chest muscle 324 30.7 320 31.0 0.30 0.495 31.35 0.370 0.599 rate, % 5 4 0 7 7
Leg muscle 380 374 365 37.3 0.44 0.675 36.34 0.419 0.705 rate, % 4 8 5 4 4
Abdominal fat 10.2 0.21 0.314 9.17 8.63 962 995 0.308 0474 rate, % 9 8 4.3 Effect of Honeycomb Extract on Immune Organ Index of Broilers
From the following table, it can be seen that adding different proportions of honeycomb extract in broiler diet has certain influence on the immune organ index of broilers. Adding 1.0%, 1.5% and 2.0% honeycomb extract to the diet increased the spleen index of broilers by 46.2%, 53.8% and 61.5% respectively, and the differences were significant compared with those of the control group (P < 0.05). Compared with the antibiotic control group, there was no significant difference in the indexes of immune organs (P < 0.05). There was no significant difference in thymus index and bursa indéx/505734 among the experimental groups (P > 0.05).
Table 4 Effects of dietary Honeycomb extract on organ index ofbroilers
Antibiot Honeycomb extract, % p-value
Project ——————Staadïars —— = ic . 0.0 rd error ANOV Linea Seconda 0.1 0.15 0.2 group A r ry
Spleen 0.27 0.38 0.40 0.42 <0.00 <0.00 0.001 0.452 0.015 index, % b a a a 1 1
Thymus 0.386 0.132 0.25 0.23 0.28 0.27 0.25 0.009 0.422 index, %
Bursa 0.225 0.229 0.14 0.13 0.15 0.15 0.15 0.005 0.588 index, % 4.4 Effect of Honeycomb Extract on Serum Immunoglobulin Content of Broilers
From the following table, it can be seen that adding different proportions of honeycomb extract to broiler diet has significant effects on serum immunoglobulin content. Adding 1.0%, 1.5% and 2.0% honeycomb extract to the diet increased the serum IgA content of broilers by 5.3%, 12.4% and 6.7% respectively, and the differences were significant compared with those of the non-antibiotic control group (P < 0.05), but there was no significant difference between them and the antibiotic control group (P > 0.05). Adding 1.0%, 1.5% and 2.0% honeycomb extract to the diet increased the serum
IgG of broilers by 2.0%, 5.4% and 4.6% respectively, and the differences were significant compared with those of the control group without antibiotics (P < 0.05), but there was no significant difference between the control group with antibiotics (P > 0.05). Adding 1.0%, 1.5% and 2.0% honeycomb extract to the diet increased the serum IgM content of broilers by 3.2%, 9.0% and 5.8%, and the differences were significant compared with those of the non-antibiotic control group (P < 0.05). There was no significant difference between the 1.0% and 2.0% honeycomb extract addition groups and the antibioti¢/505734 control group (P > 0.05).
Table 5 Effects of dietary Honeycomb extract on serum immunoglobulincontent of broilers g/L
Honeycomb extract, % p-value
Project Antibioti ————————————————— Standar —— 0.0 ANOV Secondar © group 01 015 0.2 dermor Linear
A y
IgA, 2.10 2.35 <0.00 <0.001 2.26° 2.20P 2.23° 0.016 <0.001 g/L © a 1
IgG, 0.007 0.027 4.21 409 417 431 428 0.027 0.078 g/L
IgM, 1.57 1.61P 1.70 1.65 0.001 0.003 1.6320° 0.011 0.004 g/L c c a b 4.5 Effect of Honeycomb Extract on Antioxidant Performance of Broilers
From the following table, it can be seen that adding different proportions of honeycomb extract to broiler diet has a significant effect on the antioxidant level of meat serum. Diets supplemented with 1.0%, 1.5% and 2.0% of honeycomb extract increased the serum GSH-Px levels of broilers by 19.0%, 79.4% and 62.1%, and the differences were significant compared with the control group (P < 0.05), among which the serum
GSH-Px levels in the 1.5% and 2.0% honeycomb extract groups were significantly higher than those in the antibiotic control group (P < 0.05) Adding 1.0%, 1.5% and 2.0% honeycomb extract to the diet reduced the serum MDA content of broilers by 8.0%, 25.7% and 21.2% respectively, and the difference between the 1.5% and 2.0% honeycomb extract added groups and the antibiotic control group was significant (P < 0.05), and the serum MDA content of the 1.5% honeycomb extract added group was significantly lower than that of the antibiotic control group (P < Diets supplemented with 1.0%, 1.5% and 2.0%
of honeycomb extract increased the serum T-AOC levels of broilers by 17.1%, 61.0%J505734 and 56.0%, and the differences were significant compared with those of the non-antibiotic control group (P < 0.05), among which the serum T-AOC levels of 1.5% and 2.0% of honeycomb extract groups were significantly higher than those of the antibiotic control group (P Adding 1.0%, 1.5% and 2.0% honeycomb extract to the diet increased the serum SOD level of broilers by 10.2%, 52.8% and 37.4%, and the differences were significant compared with the control group (P < 0.05), among which the serum SOD level of 1.5% and 2.0% honeycomb extract added group was significantly higher than that of antibiotic control group (P < 0.05).
Table 6 Effects of dietary Honeycomb extract on serum antioxidantcapacity in broilers
Antibio Honeycomb extract, % Standa p-value
Project - - tie 0.0 rd ANO Line Second group 0.1 015 0.2 error VA ar ary < <0.001
GSH-Px, 522.70 3724 4430 668.1 603.8 < 15.413 0.00
U/mL c 6° gd 9? 9b 0.001 1 < < <0.001
MDA, 4.94° 4.69% 5372 3.99¢ 4.23% 0.113 0.001 0.00 nmol/mL b 1 < < <0.001
T-AOC, 12.94 12.53 10.07° 8.03° 940 0.303 0.001 0.00
U/mL a a 1 < < <0.001 7452 82.09 1138 102.4
SOD, U/mL 86.40 2641 0.001 0.00 b b 7a 02 1
4 4 Effect of Honeycomb Extract on Cecal Microbes in Broilers LUS05734
From the following table, it can be seen that adding different proportions of honeycomb extract to broiler diet has significant effects on cecal microorganisms of broilers. Adding 1.0%, 1.5% and 2.0% honeycomb extract to the diet increased the total bacteria in the cecum of broilers by 7.7%, 8.1% and 10.4% respectively, and the differences were significant compared with those in the control group (P < 0.05). Adding 1.0%, 1.5% and 2.0% honeycomb extract to the diet increased the number of yeasts in the cecum of broilers by 2.5%, 7.0% and 10.0% respectively, and the difference between the 2.0% honeycomb extract added group and the control group was significant (P < 0.05). Adding 1.0%, 1.5% and 2.0% honeycomb extract to the diet increased the lactic acid bacteria in the cecum of broilers by 10.7%, 10.3% and 18.2%, respectively, and the difference between the 2.0% honeycomb extract added group and the control group was significant (P < 0.05).
Table 7 Effects of dietary Honeycomb extract on cecum microorganismsof broilers lg(CFU/g)
Honeycomb ds p-value
Project Antibi extract, % Stand otùe—_—————————————— ard ———— group 0.0 01 0.1 ga error ANO Line Second
VA ar ary
Total bacteria, 76 82 83 84 0.03 0.00 0.011 8.1229 0.086 lg(CFU/g) ob @ 19 9° 2 3
Escherichia coli, 73 68 70 6.9 0.37 0.19 0.258 7.00 0.072 lg(CFU/g) 0 4 8 7 1 2 71 72 75 T8 005 0.00 0.010
Yeast, Ig(CFU/Q) 7.46 0.081 0 8 9 0 7 3
Lactic acid bacteria, Ig 76 85 84 090 0.03 0.00 0.007 8.12° 0.142 (CFU/g) gb 12% gb ga 0 1
Although the technical scheme of the present invention has been described arid)505734 enumerated in detail, it should be understood that it is obvious for those skilled in the art to modify the above-mentioned embodiments or adopt equivalent alternatives, and these modifications or improvements made without departing from the spirit of the present invention are within the scope of the present invention.
Claims (9)
1. A method for preparing honeycomb extract, which is characterized by comprising the following steps: mixing honeycomb residue with alcohol, soaking overnight, heating to boil, cooling, filtering and concentrating to obtain honeycomb extract, and granulating the honeycomb extract to obtain coated honeycomb extract.
2. The preparation method of honeycomb extract according to claim 1, characterized in that the material-liquid ratio of honeycomb residue to alcohol is 1:2.
3. The preparation method of honeycomb extract according to clam 1, characterized in that the heating temperature is 60-100°C and the boiling time is 30-90min.
4. Honeycomb extract prepared by the method according to claim 1.
5. An application of honeycomb extract in broiler growth.
6. The application according to claim 5, the application of honeycomb extract in the growth performance of broilers.
7. The application according to claim 5, wherein the honeycomb extract is used for improving the immune function of broilers.
8. The application according to claim 5, wherein the honeycomb extract is used for improving the antioxidant function of broilers.
9. The application according to claim 5, wherein the honeycomb extract is used for improving the intestinal health of broilers.
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