RU2006108790A - Способ улучшения видовых характеристик липид-продуцирующих грибов - Google Patents
Способ улучшения видовых характеристик липид-продуцирующих грибов Download PDFInfo
- Publication number
- RU2006108790A RU2006108790A RU2006108790/13A RU2006108790A RU2006108790A RU 2006108790 A RU2006108790 A RU 2006108790A RU 2006108790/13 A RU2006108790/13 A RU 2006108790/13A RU 2006108790 A RU2006108790 A RU 2006108790A RU 2006108790 A RU2006108790 A RU 2006108790A
- Authority
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- Prior art keywords
- lipid
- gene
- strain
- mortierella
- host
- Prior art date
Links
- 241000233866 Fungi Species 0.000 claims abstract 7
- 108090000623 proteins and genes Proteins 0.000 claims abstract 7
- 150000002632 lipids Chemical class 0.000 claims abstract 6
- 239000003550 marker Substances 0.000 claims abstract 6
- ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 claims abstract 4
- 241000235575 Mortierella Species 0.000 claims abstract 3
- 230000009466 transformation Effects 0.000 claims abstract 2
- 229940035893 uracil Drugs 0.000 claims abstract 2
- 238000000034 method Methods 0.000 claims 7
- 241000907999 Mortierella alpina Species 0.000 claims 1
- 241001451064 Mortierella chlamydospora Species 0.000 claims 1
- 241000133355 Mortierella hygrophila Species 0.000 claims 1
- 108010055012 Orotidine-5'-phosphate decarboxylase Proteins 0.000 claims 1
- 239000002253 acid Substances 0.000 claims 1
- 230000000295 complement effect Effects 0.000 claims 1
- 238000004520 electroporation Methods 0.000 claims 1
- 239000002245 particle Substances 0.000 claims 1
- 241000894007 species Species 0.000 claims 1
- 239000003242 anti bacterial agent Substances 0.000 abstract 1
- 229940088710 antibiotic agent Drugs 0.000 abstract 1
- 238000009395 breeding Methods 0.000 abstract 1
- 238000011084 recovery Methods 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/02—Preparation of hybrid cells by fusion of two or more cells, e.g. protoplast fusion
- C12N15/03—Bacteria
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6436—Fatty acid esters
- C12P7/6445—Glycerides
- C12P7/6463—Glycerides obtained from glyceride producing microorganisms, e.g. single cell oil
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Cell Biology (AREA)
- Mycology (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Farming Of Fish And Shellfish (AREA)
Claims (6)
1. Способ улучшения видовых характеристик липид-продуцирующих грибов, принадлежащих роду Mortierella, включающий стадию трансформации, которая состоит из:
стадии переноса маркерного гена, где штаммом-хозяином является ауксотрофный штамм липид-продуцирующего гриба, а маркерный ген комплементарен гену, несущему ауксотрофность в штамме-хозяине;
стадии отбора трансформантов с использованием в качестве маркера восстановление прототрофности хозяина.
2. Способ по п.1, отличающийся тем, что липид-продуцирующие грибы представляют собой грибы Mortierella alpina, Mortierella hygrophila или Mortierella chlamydospora.
3. Способ по п.1 или 2, отличающийся тем, что ауксотрофный штамм представляет собой штамм, ауксотрофный по урацилу.
4. Способ по п.3, отличающийся тем, что маркерный ген представляет собой ген оротидин-5-фосфат декарбоксилазы или ген пирофосфорилазы оротидиновой кислоты.
5. Способ по п.1, отличающийся тем, что стадию переноса осуществляют путем электропорации или способом доставки частиц.
6. Способ по п.1, дополнительно включающий стадию получения ауксотрофного штамма из липид-продуцирующего гриба дикого типа.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2003299345 | 2003-08-22 | ||
| JP299345/2003 | 2003-08-22 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| RU2006108790A true RU2006108790A (ru) | 2007-10-10 |
| RU2340665C2 RU2340665C2 (ru) | 2008-12-10 |
Family
ID=34213753
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2006108790/13A RU2340665C2 (ru) | 2003-08-22 | 2004-08-20 | Способ селекции липид-продуцирующих грибов |
Country Status (14)
| Country | Link |
|---|---|
| US (1) | US20070072275A1 (ru) |
| EP (1) | EP1666584B1 (ru) |
| JP (1) | JP4537319B2 (ru) |
| KR (1) | KR101187081B1 (ru) |
| CN (1) | CN100381558C (ru) |
| AT (1) | ATE493489T1 (ru) |
| AU (1) | AU2004267294B2 (ru) |
| BR (1) | BRPI0413830A (ru) |
| CA (1) | CA2536606C (ru) |
| DE (1) | DE602004030807D1 (ru) |
| DK (1) | DK1666584T3 (ru) |
| MX (1) | MXPA06002074A (ru) |
| RU (1) | RU2340665C2 (ru) |
| WO (1) | WO2005019437A1 (ru) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2507263C2 (ru) * | 2007-05-25 | 2014-02-20 | Сантори Холдингз Лимитед | Новые гены ацилтрансферазы лизофосфатидной кислоты |
Families Citing this family (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101578366B (zh) | 2007-06-18 | 2012-05-30 | 三得利控股株式会社 | 甘油-3-磷酸酰基转移酶（gpat）同源物及其应用 |
| CN101595216B (zh) | 2007-07-11 | 2013-01-09 | 三得利控股株式会社 | 磷脂酸磷酸酶同源物及其利用 |
| BRPI0813547A2 (pt) * | 2007-07-23 | 2014-12-30 | Suntory Holdings Ltd | Composições de ácido graxo tendo uma nova taxa de ácido graxo |
| RU2534560C2 (ru) | 2007-10-26 | 2014-11-27 | Сантори Холдингз Лимитед | Новые гены атф:цитратлиазы |
| BRPI1013611A8 (pt) | 2009-03-18 | 2015-12-15 | Suntory Holdings Ltd | acetil-coa carboxilase |
| RU2534559C2 (ru) | 2009-03-26 | 2014-11-27 | Сантори Холдингз Лимитед | Новая лизофосфолипид-ацилтрансфераза |
| US8765423B2 (en) | 2009-12-21 | 2014-07-01 | Suntory Holdings Limited | Diacylglycerol acyltransferase genes and use thereof |
| EP2772538B1 (en) | 2010-02-01 | 2016-01-13 | Suntory Holdings Limited | Polynucleotide encoding acyl-CoA synthetase homolog and method for using the same |
| WO2011096481A1 (ja) | 2010-02-03 | 2011-08-11 | サントリーホールディングス株式会社 | グリセロール－３－リン酸アシル基転移酵素ホモログとその利用 |
| US8951308B2 (en) | 2011-03-17 | 2015-02-10 | Solazyme, Inc. | Pyrolysis oil and other combustible compositions from microbial biomass |
| WO2014157736A1 (ja) | 2013-03-27 | 2014-10-02 | サントリーホールディングス株式会社 | モルティエレラ属微生物内で高発現活性を示すプロモーター |
| CN103468581B (zh) * | 2013-08-09 | 2015-09-16 | 江南大学 | 一种通过同源重组敲除ura5基因的高山被孢霉尿嘧啶营养缺陷型及其构建方法 |
| CN103642698B (zh) * | 2013-11-18 | 2015-11-04 | 嘉必优生物工程（武汉）有限公司 | 高山被孢霉突变株及其应用 |
| CN105802952B (zh) * | 2016-03-29 | 2018-11-20 | 上海市农业科学院 | 一种利用尿嘧啶营养缺陷型进行香菇菌种保护的方法 |
| CN110747133B (zh) * | 2019-11-18 | 2021-03-02 | 河南农业大学 | 一株头孢被孢霉、包括头孢被孢霉的菌剂及其制备方法和应用 |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5204250A (en) * | 1986-03-31 | 1993-04-20 | Suntory Limited | Process for production of arachidonic acid |
| JPH07143894A (ja) * | 1993-11-24 | 1995-06-06 | Mitsubishi Chem Corp | タンパク質の生産方法 |
| KR100222639B1 (ko) * | 1997-07-29 | 1999-10-01 | 손경식 | 신규한 대장균 변이주 cj 181 및 그를 이용한 l-트립토판 제조방법 |
| DE19814853A1 (de) * | 1998-04-02 | 1999-10-21 | Consortium Elektrochem Ind | Expressionssystem zur Produktion von Proteinen |
| WO1999061651A2 (en) * | 1998-05-27 | 1999-12-02 | Novo Nordisk Biotech, Inc. | Methods for producing a polypeptide by modifying the copy number of a gene |
| JP2000069987A (ja) * | 1998-08-28 | 2000-03-07 | Suntory Ltd | アラキドン酸含有脂質並びにジホモ−γ−リノレン酸含有脂質の製造方法 |
| JP2000125859A (ja) * | 1998-10-26 | 2000-05-09 | Meito Sangyo Co Ltd | 新規な選択マーカー遺伝子ならびにリゾムコール・プシルスの形質転換系 |
| US6689601B2 (en) * | 2000-09-01 | 2004-02-10 | E. I. Du Pont De Nemours And Company | High growth methanotropic bacterial strain |
| US7214491B2 (en) * | 2003-05-07 | 2007-05-08 | E. I. Du Pont De Nemours And Company | Δ-12 desaturase gene suitable for altering levels of polyunsaturated fatty acids in oleaginous yeasts |
| PL1809118T3 (pl) * | 2004-11-04 | 2017-07-31 | Monsanto Technology Llc | Kompozycje oleju z nasion |
-
2004
- 2004-08-20 US US10/569,103 patent/US20070072275A1/en not_active Abandoned
- 2004-08-20 WO PCT/JP2004/012021 patent/WO2005019437A1/ja active Application Filing
- 2004-08-20 EP EP04771982A patent/EP1666584B1/en not_active Expired - Lifetime
- 2004-08-20 MX MXPA06002074A patent/MXPA06002074A/es active IP Right Grant
- 2004-08-20 RU RU2006108790/13A patent/RU2340665C2/ru not_active IP Right Cessation
- 2004-08-20 CN CNB2004800239464A patent/CN100381558C/zh not_active Expired - Fee Related
- 2004-08-20 AU AU2004267294A patent/AU2004267294B2/en not_active Ceased
- 2004-08-20 CA CA2536606A patent/CA2536606C/en not_active Expired - Fee Related
- 2004-08-20 JP JP2005513313A patent/JP4537319B2/ja not_active Expired - Fee Related
- 2004-08-20 KR KR1020067003636A patent/KR101187081B1/ko not_active Expired - Fee Related
- 2004-08-20 DK DK04771982.8T patent/DK1666584T3/da active
- 2004-08-20 DE DE602004030807T patent/DE602004030807D1/de not_active Expired - Lifetime
- 2004-08-20 AT AT04771982T patent/ATE493489T1/de not_active IP Right Cessation
- 2004-08-20 BR BRPI0413830-9A patent/BRPI0413830A/pt not_active Application Discontinuation
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2507263C2 (ru) * | 2007-05-25 | 2014-02-20 | Сантори Холдингз Лимитед | Новые гены ацилтрансферазы лизофосфатидной кислоты |
Also Published As
| Publication number | Publication date |
|---|---|
| CA2536606A1 (en) | 2005-03-03 |
| AU2004267294A1 (en) | 2005-03-03 |
| AU2004267294B2 (en) | 2008-08-28 |
| JPWO2005019437A1 (ja) | 2007-11-01 |
| ATE493489T1 (de) | 2011-01-15 |
| KR101187081B1 (ko) | 2012-09-27 |
| US20070072275A1 (en) | 2007-03-29 |
| DE602004030807D1 (de) | 2011-02-10 |
| RU2340665C2 (ru) | 2008-12-10 |
| JP4537319B2 (ja) | 2010-09-01 |
| BRPI0413830A (pt) | 2006-10-24 |
| CA2536606C (en) | 2013-04-30 |
| EP1666584B1 (en) | 2010-12-29 |
| WO2005019437A1 (ja) | 2005-03-03 |
| CN1839199A (zh) | 2006-09-27 |
| KR20070031827A (ko) | 2007-03-20 |
| CN100381558C (zh) | 2008-04-16 |
| MXPA06002074A (es) | 2006-05-25 |
| EP1666584A1 (en) | 2006-06-07 |
| DK1666584T3 (da) | 2011-02-28 |
| EP1666584A4 (en) | 2007-01-10 |
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Legal Events
| Date | Code | Title | Description |
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| PC4A | Invention patent assignment |
Effective date: 20090918 |
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| MM4A | The patent is invalid due to non-payment of fees |
Effective date: 20200821 |