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MX2012001261A - Medio de cultivo de celula para la expresion de proteina adamts. - Google Patents

Medio de cultivo de celula para la expresion de proteina adamts.

Info

Publication number
MX2012001261A
MX2012001261A MX2012001261A MX2012001261A MX2012001261A MX 2012001261 A MX2012001261 A MX 2012001261A MX 2012001261 A MX2012001261 A MX 2012001261A MX 2012001261 A MX2012001261 A MX 2012001261A MX 2012001261 A MX2012001261 A MX 2012001261A
Authority
MX
Mexico
Prior art keywords
culture medium
cell culture
protein expression
adamts protein
adamts
Prior art date
Application number
MX2012001261A
Other languages
English (en)
Inventor
Manfred Reiter
Leopold Grillberger
Meinhard Hasslacher
Alexandra Spenger
Rana Grillberger
Original Assignee
Baxter Healthcare Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Baxter Healthcare Sa filed Critical Baxter Healthcare Sa
Publication of MX2012001261A publication Critical patent/MX2012001261A/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6489—Metalloendopeptidases (3.4.24)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0681—Cells of the genital tract; Non-germinal cells from gonads
    • C12N5/0682—Cells of the female genital tract, e.g. endometrium; Non-germinal cells from ovaries, e.g. ovarian follicle cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0684—Cells of the urinary tract or kidneys
    • C12N5/0686—Kidney cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/24—Metalloendopeptidases (3.4.24)
    • C12Y304/24087—ADAMTS13 endopeptidase (3.4.24.87)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/12—Light metals, i.e. alkali, alkaline earth, Be, Al, Mg
    • C12N2500/14—Calcium; Ca chelators; Calcitonin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/22—Zinc; Zn chelators
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C12N2500/95—Protein-free medium and culture conditions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/99—Serum-free medium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Urology & Nephrology (AREA)
  • Reproductive Health (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)

Abstract

La presente invención proporciona medios de cultivo que son útiles para la expresión de proteínas ADAMTS, tales como ADAMTS13. También se proporcionan métodos para la expresión y purificación de proteínas ADAMTS. En algunas modalidades, los medios y métodos de la invención son útiles para la expresión de proteínas ADAMTS teniendo actividades altamente específicas. También se proporcionan ADAMTS, por ejemplo, ADAMTS13, composiciones de proteína con actividades altamente específicas, las cuales son expresadas y purificadas de acuerdo con los métodos aquí provistos.
MX2012001261A 2009-07-31 2010-07-30 Medio de cultivo de celula para la expresion de proteina adamts. MX2012001261A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US23047709P 2009-07-31 2009-07-31
PCT/US2010/044020 WO2011014838A1 (en) 2009-07-31 2010-07-30 Cell culture medium for adamts protein expression

Publications (1)

Publication Number Publication Date
MX2012001261A true MX2012001261A (es) 2012-05-22

Family

ID=42668009

Family Applications (2)

Application Number Title Priority Date Filing Date
MX2012001261A MX2012001261A (es) 2009-07-31 2010-07-30 Medio de cultivo de celula para la expresion de proteina adamts.
MX2013009470A MX344970B (es) 2009-07-31 2012-01-27 Medio de cultivo celular para la expresion de la proteina adamts.

Family Applications After (1)

Application Number Title Priority Date Filing Date
MX2013009470A MX344970B (es) 2009-07-31 2012-01-27 Medio de cultivo celular para la expresion de la proteina adamts.

Country Status (22)

Country Link
US (7) US8313926B2 (es)
EP (1) EP2459702B1 (es)
JP (5) JP5451884B2 (es)
KR (5) KR101808496B1 (es)
CN (2) CN104962539B (es)
AU (1) AU2010278751B2 (es)
BR (2) BR112012002151B1 (es)
CA (1) CA2769361A1 (es)
CO (1) CO6501191A2 (es)
EA (1) EA023193B1 (es)
ES (1) ES2583258T3 (es)
HK (1) HK1171790A1 (es)
HR (1) HRP20160783T1 (es)
HU (1) HUE029110T2 (es)
IN (1) IN2012DN00801A (es)
MX (2) MX2012001261A (es)
NZ (1) NZ597727A (es)
PL (1) PL2459702T3 (es)
PT (1) PT2459702T (es)
SG (1) SG178194A1 (es)
SI (1) SI2459702T1 (es)
WO (1) WO2011014838A1 (es)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
HUE029110T2 (en) * 2009-07-31 2017-02-28 Baxalta GmbH Cell culture medium for ADAMTS protein expression
US9458222B2 (en) 2010-07-08 2016-10-04 Baxalta Incorporated Method of producing recombinant ADAMTS13 in cell culture
ES2788132T3 (es) 2011-07-01 2020-10-20 Amgen Inc Cultivo de células de mamífero
ES2633960T3 (es) 2012-10-15 2017-09-26 Bristol-Myers Squibb Company Procesos de cultivo de células de mamífero para la producción de proteínas
US9611455B2 (en) 2013-03-13 2017-04-04 Merck Sharp & Dohme Corp. Adapted lepidopteran insect cells for the production of recombinant proteins
US9217168B2 (en) 2013-03-14 2015-12-22 Momenta Pharmaceuticals, Inc. Methods of cell culture
AR095196A1 (es) * 2013-03-15 2015-09-30 Regeneron Pharma Medio de cultivo celular libre de suero
WO2015055324A1 (en) * 2013-10-14 2015-04-23 Ares Trading S.A New medium for high performance mammalian fed-batch cultures
CN106687575B (zh) 2014-06-04 2021-04-30 美国安进公司 用于收获哺乳动物细胞培养物的方法
KR101867134B1 (ko) * 2015-03-23 2018-06-12 한양대학교 산학협력단 포유류 세포를 이용하여 목적 물질을 고효율로 생산하기 위한 세포 배양 배지, 이를 이용한 세포 배양 방법 및 목적 물질의 생산 방법
CN107636151B (zh) * 2015-05-22 2023-04-04 杜邦营养生物科学有限公司 乙酰乳酸脱羧酶
TW202440903A (zh) 2015-08-04 2024-10-16 美商再生元醫藥公司 補充牛磺酸之細胞培養基及用法（一）
EP3493830A1 (en) 2016-08-04 2019-06-12 Baxalta Incorporated Use of adamts13 for treating, ameliorating and/or preventing vaso-occlusive crisis in sickle cell disease, acute lung injury and/or acute respiratory distress syndrome
CN110168081B (zh) * 2016-11-04 2024-08-16 武田药品工业株式会社 腺相关病毒纯化方法
CN114075269A (zh) * 2017-07-06 2022-02-22 菲仕兰坎皮纳荷兰私人有限公司 用于制备糖蛋白的细胞培养工艺
JP2021515584A (ja) * 2018-03-16 2021-06-24 ジェンザイム・コーポレーション 産生バイオリアクターにおける細胞生存度を改善するための方法
EP3807408A4 (en) * 2018-06-18 2022-03-30 Synthetic Biologics, Inc. METHODS OF MAKING ALKALINE PHOSPHATASE AGENTS
WO2021067668A1 (en) 2019-10-04 2021-04-08 The Children's Medical Center Corporation Adamts13 treatment to enhance graft survival
MY190627A (en) 2019-12-06 2022-04-27 Regeneron Pharma Anti-vegf protein compositions and methods for producing the same
US20230203469A1 (en) 2020-04-02 2023-06-29 Takeda Pharmaceutical Company Limited Adamts13 variant, compositions, and uses thereof
CN115803009A (zh) 2020-05-08 2023-03-14 瑞泽恩制药公司 用于治疗眼病和癌症的vegf阱和微阱及方法
US20230201319A1 (en) 2020-05-22 2023-06-29 Takeda Pharmaceutical Company Limited Adamts13 compositions and methods for treating and diagnosing complications of coronavirus disease
US20230020297A1 (en) * 2020-06-05 2023-01-19 Proabtech Co., Ltd. Uox-albumin conjugate with certain numbers of albumin conjugated thereto, and manufacturing method thereof
CN111808822B (zh) * 2020-07-02 2024-01-12 北京百普赛斯生物科技股份有限公司 细胞培养用补料液及提高重组hek293细胞蛋白表达量的方法

Family Cites Families (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4797368A (en) 1985-03-15 1989-01-10 The United States Of America As Represented By The Department Of Health And Human Services Adeno-associated virus as eukaryotic expression vector
US5139941A (en) 1985-10-31 1992-08-18 University Of Florida Research Foundation, Inc. AAV transduction vectors
US5250421A (en) 1986-01-03 1993-10-05 Genetics Institute, Inc. Method for producing factor VIII:C-type proteins
JPH0753104B2 (ja) * 1989-02-28 1995-06-07 秀明 山田 細菌の培養法
CZ280901B6 (cs) 1988-10-06 1996-05-15 Hideaki Yamada Způsob kultivace bakterií
GB9022545D0 (en) 1990-10-17 1990-11-28 Wellcome Found Culture medium
DK1213030T3 (da) 1994-11-10 2009-07-20 Baxter Healthcare Sa Fremgangsmåde til fremstilling af biologiske produkter i protein-fri kultur
JP3649249B2 (ja) 1995-02-23 2005-05-18 クエスト・インターナショナル・サービシーズ・ビー・ブイ 組織および細胞培養用ペプチド
US6013516A (en) 1995-10-06 2000-01-11 The Salk Institute For Biological Studies Vector and method of use for nucleic acid delivery to non-dividing cells
EP2221361A3 (en) 1996-08-30 2011-02-09 Life Technologies Corporation Method for producing a polypeptide in vitro in mammalian cells in a protein-free and serum-free culture medium
IT1285158B1 (it) * 1996-09-17 1998-06-03 Angeletti P Ist Richerche Bio Polipeptidi solubili con l'attivita' di serino-proteasi di ns3 del virus dell'epatite c, e procedimento per la loro preparazione e il
DE69717650T2 (de) 1996-10-07 2003-09-18 Solutia Inc., St. Louis Verfahren zur hydroxylierung von benzol
JP4543402B2 (ja) 1996-10-10 2010-09-15 ライフ テクノロジーズ コーポレーション 植物由来栄養素を含む動物細胞培養培地
US5804420A (en) 1997-04-18 1998-09-08 Bayer Corporation Preparation of recombinant Factor VIII in a protein free medium
US6475725B1 (en) 1997-06-20 2002-11-05 Baxter Aktiengesellschaft Recombinant cell clones having increased stability and methods of making and using the same
AT407255B (de) 1997-06-20 2001-02-26 Immuno Ag Rekombinanter zellklon mit erhöhter stabilität in serum- und proteinfreiem medium und verfahren zur gewinnung des stabilen zellklons
US5994136A (en) 1997-12-12 1999-11-30 Cell Genesys, Inc. Method and means for producing high titer, safe, recombinant lentivirus vectors
US6406909B1 (en) 1998-07-10 2002-06-18 Chugai Seiyaku Kabushiki Kaisha Serum-free medium for culturing animal cells
AT409379B (de) 1999-06-02 2002-07-25 Baxter Ag Medium zur protein- und serumfreien kultivierung von zellen
US6926894B2 (en) 2000-11-22 2005-08-09 Baxter Aktiengesellschaft Composition exhibiting a von willebrand factor (vWF) protease activity comprising a polypeptide chain with the amino acid sequence AAGGILHLELLV
WO2004056976A2 (en) 2002-12-23 2004-07-08 Fuji Photo Film B.V. Process for coating cell-culture support
EP1737953A2 (en) * 2004-04-16 2007-01-03 Wyeth a Corporation of the State of Delaware Adamts-8 proteins and uses thereof
US20060094104A1 (en) 2004-10-29 2006-05-04 Leopold Grillberger Animal protein-free media for cultivation of cells
JP2007174948A (ja) * 2005-12-27 2007-07-12 Noritsu Koki Co Ltd 酵素水製造装置
JP4880997B2 (ja) * 2005-12-28 2012-02-22 アルフレッサファーマ株式会社 Ａｄａｍｔｓ１３の安定化方法
PL1974014T3 (pl) * 2006-01-04 2017-09-29 Baxalta Incorporated Wolne od oligopeptydów pożywki do hodowli komórkowych
WO2008077217A2 (en) * 2006-12-22 2008-07-03 Springer Carrier Ltda. Fresh air command lever for an air conditioning unit
US20090067805A1 (en) 2007-09-10 2009-03-12 Victor Company Of Japan, Limited Recording and reproducing device
SI2574677T1 (sl) * 2007-12-27 2017-11-30 Baxalta GmbH Procesi celične kulture
HUE029110T2 (en) * 2009-07-31 2017-02-28 Baxalta GmbH Cell culture medium for ADAMTS protein expression

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IN2012DN00801A (es) 2015-06-26
BR112012002151A2 (pt) 2020-03-03
NZ597727A (en) 2013-08-30
JP7083059B2 (ja) 2022-06-09
JP2021112210A (ja) 2021-08-05
US20170051270A1 (en) 2017-02-23
US20200308566A1 (en) 2020-10-01
HK1171790A1 (zh) 2013-04-05
SG178194A1 (en) 2012-03-29
JP2014061005A (ja) 2014-04-10
JP2016144471A (ja) 2016-08-12
EP2459702A1 (en) 2012-06-06
CA2769361A1 (en) 2011-02-03
US9441216B2 (en) 2016-09-13
KR101912552B1 (ko) 2018-10-26
JP5451884B2 (ja) 2014-03-26
EA201270224A1 (ru) 2012-09-28
KR101573972B1 (ko) 2015-12-02
KR20170140411A (ko) 2017-12-20
US8313926B2 (en) 2012-11-20
CO6501191A2 (es) 2012-08-15
JP6567707B2 (ja) 2019-08-28
BR112012002151B1 (pt) 2021-07-06
HUE029110T2 (en) 2017-02-28
JP6883071B2 (ja) 2021-06-09
JP6511007B2 (ja) 2019-05-08
CN102549145B (zh) 2015-05-20
SI2459702T1 (sl) 2016-11-30
US10724024B2 (en) 2020-07-28
EA023193B1 (ru) 2016-05-31
US20140329294A1 (en) 2014-11-06
KR20150138425A (ko) 2015-12-09
US10072254B2 (en) 2018-09-11
PT2459702T (pt) 2016-08-02
US11254921B2 (en) 2022-02-22
JP2019201661A (ja) 2019-11-28
KR20170081747A (ko) 2017-07-12
CN102549145A (zh) 2012-07-04
US20150329848A1 (en) 2015-11-19
CN104962539A (zh) 2015-10-07
HRP20160783T1 (hr) 2016-08-12
US20110086413A1 (en) 2011-04-14
KR101808496B1 (ko) 2017-12-12
AU2010278751A1 (en) 2012-02-09
US8759026B2 (en) 2014-06-24
MX344970B (es) 2017-01-12
KR20150044981A (ko) 2015-04-27
JP2013500724A (ja) 2013-01-10
KR20120046301A (ko) 2012-05-09
KR101756354B1 (ko) 2017-07-26
JP5936232B2 (ja) 2016-06-22
EP2459702B1 (en) 2016-05-04
PL2459702T3 (pl) 2016-12-30
JP2018093881A (ja) 2018-06-21
WO2011014838A1 (en) 2011-02-03
CN104962539B (zh) 2019-09-17
ES2583258T3 (es) 2016-09-20
US20190071660A1 (en) 2019-03-07
US20130071906A1 (en) 2013-03-21
BR122021005965B1 (pt) 2022-01-25
KR101574056B1 (ko) 2015-12-02
AU2010278751B2 (en) 2014-05-15
US9127265B2 (en) 2015-09-08

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