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KR970006507A - 미생물 검출 방법 - Google Patents

미생물 검출 방법 Download PDF

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Publication number
KR970006507A
KR970006507A KR1019960029720A KR19960029720A KR970006507A KR 970006507 A KR970006507 A KR 970006507A KR 1019960029720 A KR1019960029720 A KR 1019960029720A KR 19960029720 A KR19960029720 A KR 19960029720A KR 970006507 A KR970006507 A KR 970006507A
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hemolymph
microorganism
sample
react
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KR1019960029720A
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KR100264366B1 (ko
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마사까즈 쓰찌야
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다나까 모또아끼
와꼬 쥰야꾸 고오교 가부시끼가이샤
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor

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Abstract

시료중의 미생물은 시료를 필터로 여과하고, 이어서 필터를 세척하며, 필터상의 잔류물을 프로-페놀 산화효소 캐스케이트의 불활성형 인자를 함유하는 곤충 혈림프와 반응시켜 이에 따른 변색을 근거로하여 시료중의 미생물을 검출하는 것을 특징으로 하는 방법으로 고정확성으로 쉽고 빠르게 검출할 수 있다.

Description

미생물 검출 방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도은 실시예 1에서 수득한 락토바실루스 델부릭키이(Lactobacillus delbrueckii)의 검출 결과이다, 제2도는 실시예 1에서 수득한 대장균의 검출 결과이다, 제3도는 실시예 2에서 수득한 대장균의 검출 결과이다, 제5도는 시료에 락토바실루스 델부릭키이가 함유된 실시예 3에서 얻은 결과를 나타내는 그래프이다, 제7도은 실시예 4에서 수득한 결과를 나타내는 그래프이다.

Claims (4)

  1. 미생물을 함유하는 시료를 필터로 여과하고, 이어서 필터를 세척하여, 필터상의 잔류물과 프로-페놀 산화효소 캐스케이드의 불활성형 인자를 함유하는 곤충 혈림프와 반응시키고, 반응에 의한 변색에 기초하여 시료내의 미생물을 검출하는 것을 특징으로 하는 미생물 검출 방법.
  2. 제1항에 있어서, 곤충 혈림프는 β-글루칸과는 반응하나 펩티도글리칸과는 반응하지 않는 혈림프인 방법.
  3. 제1항에 있어서, 곤충 혈림프는 펩티도글리칸과는 반응하나 β-글루칸과는 반응하지 않는 혈림프인 방법.
  4. 제1항에 있어서, 변색을 육안으로 관찰하는 방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019960029720A 1995-07-31 1996-07-23 미생물 검출 방법 KR100264366B1 (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP21420095 1995-07-31
JP95-214200 1995-07-31

Publications (2)

Publication Number Publication Date
KR970006507A true KR970006507A (ko) 1997-02-21
KR100264366B1 KR100264366B1 (ko) 2000-08-16

Family

ID=16651895

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KR1019960029720A KR100264366B1 (ko) 1995-07-31 1996-07-23 미생물 검출 방법

Country Status (8)

Country Link
US (1) US5747277A (ko)
EP (1) EP0757103B1 (ko)
KR (1) KR100264366B1 (ko)
CN (1) CN1091467C (ko)
CA (1) CA2181325A1 (ko)
DE (1) DE69610537T2 (ko)
ES (1) ES2150637T3 (ko)
TW (1) TW409149B (ko)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20040012270A (ko) * 2002-08-02 2004-02-11 이재영 섬유원단의 감량가공장치
KR100456006B1 (ko) * 2000-08-24 2004-11-08 주식회사 삼양제넥스 페놀옥시데이즈 시스템을 구성하는 단백질 및 그것의 유전자
KR100471308B1 (ko) * 2001-06-08 2005-03-08 주식회사 삼양제넥스 펩티도글리칸 검출용 조성물, 및 이를 이용한펩티도글리칸 검출키트

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1108385C (zh) * 1998-07-24 2003-05-14 中国科学院感光化学研究所 一种具有识别病菌、病毒功能的光敏泡囊及其制法
US7108980B1 (en) * 1998-08-07 2006-09-19 Boston Probes, Inc. Methods for the analysis of microcolonies of bacteria and yeast
US6790661B1 (en) * 1999-07-16 2004-09-14 Verax Biomedical, Inc. System for detecting bacteria in blood, blood products, and fluids of tissues
AU2891401A (en) * 2000-01-20 2001-07-31 Samyang Genex Corporation Composition for detecting beta-1,3-glucan, preparation method thereof and diagnostic kit detecting beta-1,3-glucan
KR100467165B1 (ko) * 2000-01-20 2005-01-24 주식회사 삼양제넥스 β-1,3-글루칸 검출용 조성물, 그것의 제조방법 및 그것을이용한 β-1,3-글루칸 검출 키트
WO2002101083A1 (en) * 2001-06-08 2002-12-19 Samyang Genex Corporation Composition for detecting peptidoglycan, and diagnostic kit detecting peptidoglycan
KR100485947B1 (ko) * 2001-07-14 2005-04-28 주식회사 삼양제넥스 β-1,3-글루칸 검출용 조성물, 그것의 제조방법 및 그것을이용한 β-1,3-글루칸 검출 키트
US8450079B2 (en) 2003-10-31 2013-05-28 Immunetics, Inc. Method for detecting bacteria
US7598054B2 (en) 2003-10-31 2009-10-06 Immunetics, Inc. Rapid peptidoglycan-based assay for detection of bacterial contamination of platelets
US20050239045A1 (en) * 2003-12-08 2005-10-27 Arkray Inc. Microorganism or cell collecting method, and microorganism or cell collecting implement used for the method
US7993821B2 (en) * 2005-08-11 2011-08-09 University Of Washington Methods and apparatus for the isolation and enrichment of circulating tumor cells
US8173413B2 (en) 2005-08-11 2012-05-08 University Of Washington Separation and concentration of biological cells and biological particles using a one-dimensional channel
US8841135B2 (en) 2007-06-20 2014-09-23 University Of Washington Biochip for high-throughput screening of circulating tumor cells
KR101802422B1 (ko) 2015-04-08 2017-11-28 해성바이오 주식회사 펩티도글리칸 결합단백질을 이용한 박테리아 검출용 프로브 및 그의 이용

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE431228B (sv) * 1981-12-17 1984-01-23 Kenneth Soderhell Sett att bestemma bakteriella endotoxin medelst profenoloxidashaltigt blodkroppslysat fran kreftdjur eller insekter och reagens herfor
US5081017A (en) * 1986-02-18 1992-01-14 Texas Bioresource Corporation Method and apparatus for detection and quantitation of bacteria
DE3751109T2 (de) * 1986-12-03 1995-10-12 Wako Pure Chem Ind Ltd Reagenzien zur Bestimmung von Peptidoglykan und beta-1,3-Glukan.
EP0657546B1 (en) * 1993-11-18 2002-03-06 Wako Pure Chemical Industries, Ltd. Method for assaying activity of prophenoloxidase activating enzyme and application thereof

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100456006B1 (ko) * 2000-08-24 2004-11-08 주식회사 삼양제넥스 페놀옥시데이즈 시스템을 구성하는 단백질 및 그것의 유전자
KR100471308B1 (ko) * 2001-06-08 2005-03-08 주식회사 삼양제넥스 펩티도글리칸 검출용 조성물, 및 이를 이용한펩티도글리칸 검출키트
KR20040012270A (ko) * 2002-08-02 2004-02-11 이재영 섬유원단의 감량가공장치

Also Published As

Publication number Publication date
CN1149628A (zh) 1997-05-14
TW409149B (en) 2000-10-21
CA2181325A1 (en) 1997-02-01
KR100264366B1 (ko) 2000-08-16
EP0757103B1 (en) 2000-10-04
ES2150637T3 (es) 2000-12-01
DE69610537D1 (de) 2000-11-09
US5747277A (en) 1998-05-05
CN1091467C (zh) 2002-09-25
DE69610537T2 (de) 2001-06-07
EP0757103A3 (en) 1997-12-29
EP0757103A2 (en) 1997-02-05

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