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KR950018454A - 단백질 c를 생성시키는 방법 - Google Patents

단백질 c를 생성시키는 방법 Download PDF

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Publication number
KR950018454A
KR950018454A KR1019940034085A KR19940034085A KR950018454A KR 950018454 A KR950018454 A KR 950018454A KR 1019940034085 A KR1019940034085 A KR 1019940034085A KR 19940034085 A KR19940034085 A KR 19940034085A KR 950018454 A KR950018454 A KR 950018454A
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KR
South Korea
Prior art keywords
host cell
recombinant
temperature
protein
incubation temperature
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Ceased
Application number
KR1019940034085A
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English (en)
Inventor
윌리엄 그린넬 브라이언
Original Assignee
피터 지. 스트링거
일라이 릴리 앤드 캄파니
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Application filed by 피터 지. 스트링거, 일라이 릴리 앤드 캄파니 filed Critical 피터 지. 스트링거
Publication of KR950018454A publication Critical patent/KR950018454A/ko
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/745—Blood coagulation or fibrinolysis factors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6464—Protein C (3.4.21.69)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2523/00—Culture process characterised by temperature

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Hematology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

본 발명은 세포를 약 38℃ 내지 약 39℃의 온도에서 배양함으로써 아데노바이러스-형질전환된 포유동물 세포중에서 작용성 재조합 단백질을 고수준으로 생성시키는 방법에 관한 것이다. 상기 방법은 어떤 세포계에서는 총 단백질 발현 수준이 증가되도록 하고, 다른 세포계에서는 작용성 단백질의 수준이 증가되도록 한다.

Description

단백질 C를 생성시키는 방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (10)

  1. 아데노바이러스-혈질전환된 재조합 포유동물 숙주 세포를 약 38℃ 내지 39℃의 온도에서 배양함을 포함하는, 상기 아데노바이러스-형질전환된 재조합 포유동물 숙주 세포에서 단백질 C의 생성을 증가시키기 위한 방법.
  2. 제1항에 있어서, 상기 재조합 포유동물 숙주 세포가 293 세포인 방법.
  3. 제1항에 있어서, 상기 배양 온도가 약 38℃인 방법.
  4. 제1항에 있어서, 상기 배양 온도가 약 39℃인 방법.
  5. 제2항에 있어서, 상기 배양 온도가 약 38℃인 방법.
  6. 제2항에 있어서, 상기 배양 온도가 약 38.5℃인 방법.
  7. 제2항에 있어서, 상기 배양 온도가 약 39℃인 방법.
  8. 재조합 AV12숙주 세포중의 재조합적으로 생성된 단백질 C분자를 약 38℃ 내지 약 39℃의 온도에서 배양함을 포함하는, 상기 재조합적으로 생성된 단백질 C분자의-카복실화 수준을 증가시키는 방법.
  9. 제8항에 있어서, 상기 재조합 AV12 숙주 세포를 약 38℃에서 배양하는 방법.
  10. 제8항에 있어서, 상기 재조합 AV12 숙주 세포를 약 39℃에서 배양하는 방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019940034085A 1993-12-15 1994-12-14 단백질 c를 생성시키는 방법 Ceased KR950018454A (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/168,035 US5618714A (en) 1993-12-15 1993-12-15 Methods for producing protein C
US08/168.035 1993-12-15

Publications (1)

Publication Number Publication Date
KR950018454A true KR950018454A (ko) 1995-07-22

Family

ID=22609820

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019940034085A Ceased KR950018454A (ko) 1993-12-15 1994-12-14 단백질 c를 생성시키는 방법

Country Status (28)

Country Link
US (1) US5618714A (ko)
EP (1) EP0658626B1 (ko)
JP (1) JP3662614B2 (ko)
KR (1) KR950018454A (ko)
CN (1) CN1107887A (ko)
AT (1) ATE196165T1 (ko)
AU (1) AU687914B2 (ko)
BR (1) BR9404991A (ko)
CA (1) CA2138101A1 (ko)
CO (1) CO4340654A1 (ko)
CZ (2) CZ315794A3 (ko)
DE (1) DE69425809T2 (ko)
DK (1) DK0658626T3 (ko)
ES (1) ES2149244T3 (ko)
FI (1) FI106385B (ko)
GR (1) GR3034909T3 (ko)
HU (1) HU219508B (ko)
IL (1) IL111983A0 (ko)
NO (1) NO944846L (ko)
NZ (1) NZ270140A (ko)
PE (1) PE42695A1 (ko)
PL (1) PL176381B1 (ko)
PT (1) PT658626E (ko)
RU (1) RU2110574C1 (ko)
SI (1) SI0658626T1 (ko)
UA (1) UA26870C2 (ko)
YU (1) YU73494A (ko)
ZA (1) ZA949981B (ko)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19724543A1 (de) * 1997-06-11 1998-12-17 Philips Patentverwaltung Wechsler-Gerät für Informationsplatten
ATE400030T1 (de) 2001-02-19 2008-07-15 Merck Patent Gmbh Methode zur identifizierung von t-zellepitopen und deren anwendung zur herstellung von molekülen mit reduzierter immunogenität
ATE386824T1 (de) * 2002-03-29 2008-03-15 Merck & Co Inc Verfahren zur virusproduktion
US20070142272A1 (en) * 2003-01-24 2007-06-21 Zlokovic Berislav V Neuroprotective activity of activated protein c independent of its anticoagulant activity
US20080305100A1 (en) * 2004-07-23 2008-12-11 Zlokovic Berislav V Activated Protein C Inhibits Undesirable Effects of Plasminogen Activator in the Brain
WO2006136033A1 (en) * 2005-06-23 2006-12-28 The University Of British Columbia Coagulation factor iii polymorphisms associated with prediction of subject outcome and response to therapy
CA2654761A1 (en) * 2006-06-09 2007-12-13 The University Of British Columbia Interferon gamma polymorphisms as indicators of subject outcome in critically ill subjects
WO2009089620A1 (en) * 2008-01-15 2009-07-23 The Universityof British Columbia Protein c rs2069915 as a response predictor to survival and administration of activated protein c or protein c-like compound
WO2012068519A2 (en) 2010-11-19 2012-05-24 Sirius Genomics Inc. Markers associated with response to activated protein c administration, and uses thereof

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4775624A (en) * 1985-02-08 1988-10-04 Eli Lilly And Company Vectors and compounds for expression of human protein C
US4959318A (en) * 1985-06-27 1990-09-25 Zymogenetics, Inc. Expression of protein C
US4968626A (en) * 1985-08-15 1990-11-06 Board Of Reagents Of The University Of Washington DNA sequence coding for protein C
EP0245949B1 (en) * 1986-04-09 1997-10-29 Eli Lilly And Company A method of using eukaryotic expression vectors comprising the bk virus enhancer
JPH01502080A (ja) * 1986-11-17 1989-07-27 ニュー・イングランド・メディカル・センター 組み換えビタミンｋ依存性蛋白質のガンマカルボキシル化の増強
US4992373A (en) * 1987-12-04 1991-02-12 Eli Lilly And Company Vectors and compounds for direct expression of activated human protein C
ZA889497B (en) * 1987-12-28 1990-08-29 Lilly Co Eli Vectors and compounds for expression of zymogen forms of human protein c
JPH0246296A (ja) * 1988-08-09 1990-02-15 Hoechst Japan Ltd 雑種プロテインｃ及びその製造方法
US4981952A (en) * 1988-10-04 1991-01-01 Eli Lilly And Company Method for the purification of vitamin K-dependent proteins
CA1332049C (en) * 1988-10-07 1994-09-20 Eli Lilly And Company Eukaryotic expression
JP3045307B2 (ja) * 1989-08-11 2000-05-29 ザイモジェネティクス，インコーポレイティド 活性化プロテインｃを生成するための細胞培養法
IL97312A (en) * 1990-02-23 1999-01-26 Lilly Co Eli A method of generating a polypeptide in an eukaryotic vector AND recombinant surrogate cell containing an enhanced transcriptional control unit based on the primary late adenovirus coefficient
EP0530365A1 (en) * 1991-01-29 1993-03-10 Teijin Limited Human protein c expression vector

Also Published As

Publication number Publication date
NO944846D0 (no) 1994-12-14
BR9404991A (pt) 1995-08-08
IL111983A0 (en) 1995-03-15
FI945877L (fi) 1995-06-16
ES2149244T3 (es) 2000-11-01
SI0658626T1 (en) 2001-02-28
CZ290724B6 (cs) 2002-10-16
EP0658626A1 (en) 1995-06-21
DE69425809D1 (de) 2000-10-12
AU8045294A (en) 1995-06-22
PL176381B1 (pl) 1999-05-31
EP0658626B1 (en) 2000-09-06
FI106385B (fi) 2001-01-31
HU9403600D0 (en) 1995-02-28
ZA949981B (en) 1996-06-14
YU73494A (sh) 1997-03-07
RU2110574C1 (ru) 1998-05-10
HU219508B (hu) 2001-04-28
RU94043769A (ru) 1997-03-27
FI945877A0 (fi) 1994-12-14
US5618714A (en) 1997-04-08
CZ315794A3 (en) 1995-10-18
HUT69979A (en) 1995-09-28
NZ270140A (en) 1996-05-28
DK0658626T3 (da) 2000-10-16
JP3662614B2 (ja) 2005-06-22
CN1107887A (zh) 1995-09-06
GR3034909T3 (en) 2001-02-28
UA26870C2 (uk) 1999-12-29
PE42695A1 (es) 1995-12-12
NO944846L (no) 1995-06-16
DE69425809T2 (de) 2001-02-01
AU687914B2 (en) 1998-03-05
JPH07222589A (ja) 1995-08-22
CO4340654A1 (es) 1996-07-30
PL306291A1 (en) 1995-06-26
ATE196165T1 (de) 2000-09-15
PT658626E (pt) 2001-01-31
CA2138101A1 (en) 1995-06-16

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