[go: up one dir, main page]

KR890701764A - 표적 뉴클레오티드 서열의 선택적 증식 - Google Patents

표적 뉴클레오티드 서열의 선택적 증식

Info

Publication number
KR890701764A
KR890701764A KR1019890700555A KR890700555A KR890701764A KR 890701764 A KR890701764 A KR 890701764A KR 1019890700555 A KR1019890700555 A KR 1019890700555A KR 890700555 A KR890700555 A KR 890700555A KR 890701764 A KR890701764 A KR 890701764A
Authority
KR
South Korea
Prior art keywords
sequence
primer
target
complementary
stranded
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
KR1019890700555A
Other languages
English (en)
Other versions
KR960013577B1 (ko
Inventor
로렌스 제임스 버그
필립 쟈크 파울레티
죤 챨스 브루쓰로이드
Original Assignee
원본미기재
더 보오드 오브 트러스티스 오브 더 릴랜드 스탠포드 쥬니어 유니버시티
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=22157643&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=KR890701764(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by 원본미기재, 더 보오드 오브 트러스티스 오브 더 릴랜드 스탠포드 쥬니어 유니버시티 filed Critical 원본미기재
Publication of KR890701764A publication Critical patent/KR890701764A/ko
Application granted granted Critical
Publication of KR960013577B1 publication Critical patent/KR960013577B1/ko
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C12N15/69—Increasing the copy number of the vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6893—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for protozoa

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Cell Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Nitrogen And Oxygen Or Sulfur-Condensed Heterocyclic Ring Systems (AREA)
  • Traffic Control Systems (AREA)
  • Multicomponent Fibers (AREA)

Abstract

내용 없음

Description

표적 뉴클레오티드 서열의 선택적 증식
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
본 발명은 본 명세서의 일부를 구성하는 도면을 참고로 하는 본 발명의 하기의 상세한 설명에 의해 더욱 이해될 수 있을 것이다. 도면은 본 발명의 방법의 각 단계에서 존재하는 폴리누클레오티드를 나타내는 도식적인 도표이다.

Claims (15)

  1. (1) 표적 단일 가닥 폴리누클레오티드 분자를 프라이머서열과 서열 혼성화하고, 적어도 하나의 프라이머가 프로모터서열을 함유하는 프라이머를 연장 및 변성시켜 표적서열의 상류에 프로모터서열을 갖는 이중가닥 DNA 중간체를 제조하고, (2) 상기 프로모터를 결합시킬 수 있는 RNA 폴리머라제를 사용하여 상기 중간체로부터 표적서열의 다중 RNA 복제물을 성장시킴을 특징으로 하는, 반응 매질에서 표적 폴리누클레오티드 서열의 복제수의 증식방법.
  2. 제1항에 있어서, (1) 상기 RNA 복제물을 프라이머서열과 혼성화, 연장 및 변성시킴으로써 상기 RNA복제물로부터 표적서열의 상류에 프로모터서열을 갖는 이중가닥 DNA 중간체의 제2콜렉션을 제조하고, (2) 상기 이중가닥 DNA 중간체의 제2콜렉션으로부터 다중 DNA 복제물을 성장시킴을 특징으로 하는 방법.
  3. 제1항에 있어서, 연장이 반응매질을 역전사효소와 접촉시키는 방법인 방법.
  4. 제1항에 있어서, 이중가닥 표적분자의 안티-센스가닥을 그의 5' 말단의 프로모터서열 및 표적서열의 5'말단의 표적분자 센스가닥의 서열과 동등한 결합서열을 함유하는 제1프라이머와 혼성화시키고, 상기 안티-센스가닥을 템플레이트로 사용하여 제1프라이머로부터 제1상보적가닥을 연장시키고, 변성시켜 단일가닥 폴리누클레오티드 중간체의 제1콜렉션을 형성하고 제1콜렉션을 상기 센스가닥의 표적서열의 3' 말단에 결합하는 제2프라이머와 혼성화하고, 상기 제1상보적가닥을 템플레이트로 사용하여 상기 제2프라이머로부터 상보적가닥을 연장시켜 상기 이중가닥 중간체를 제공하고, 상기 반응매질은 프로모터를 결합할 수 있는 RNA 폴리머라제와 접촉시킴을 특징으로 하는 방법.
  5. 제1항에 있어서, (1) 서열 X1-P1-T-P2-X2의 표적분자에 상보적인 서열 X1'-P1'-T'-P2'-X2'의 단일가닥 폴리누클레오티드 분자를 서열 PR-P1의 제1프라이머와 혼성화하여
    를 형성하고 : (2) 상보적가닥을 연장시켜
    PR
    P1--T--P2--X2
    X1'-P1'-T'-P2'-X2'
    를 형성하고, 변성시켜 단일가닥 폴리누클레오티드의 제1콜렉션을 형성하고 : (3) 이 단일가닥 폴리누클레오티드의 제1콜렉션을 서열 P2'의 제2프라이머와 혼성화시켜
    PR-P1-T-P2-S2
    P2'
    를 형성하고 : (4) 상보적가닥을 연장시켜
    PR-P1--T--P2--X2
    PR'-P1'-T'-P2'-X2'
    를 형성하고 : (5) 이중가닥 프로모터 부분
    PR
    PR'
    을 결합할 수 있는 RNA 플리머라제를 사용하여 식 P1R-TR-P2R을 갖는 P1-T-P2의 다중RNA 복제물을 성장시킴을 특징으로 하는 방법. (상기식중 : T는 표적서열이고 : T'는 T에 상보적인 서열이며 : PR-P1은 PR이 프로모터서열이고, PO1이 제1프라이머의 결합서열인 제1올리고누클레오티드 프라이머이고 : P1'는 P1에 상보적인 서열이며 : PR'는 PR에 상보적인 서열이고 : P2' : 는 제2올리고 누클레오티드 프라이머이며 : P2는 P2'에 상보적인 표적 폴리누클레오티드의 서열이고 : X1 및 X2는 각각 존재 또는 부재할 수 있는 상기 표적분자의 P1-T-P2 서열밖의 서열이며 : X1'는 X1에 상보적인 서열이고 : X2'는 X2에 상보적인 서열이며 : P1R-TR-P2R은 P1R이 P1에 상응하는 서열이고, YR이 T에 상응하는 서열이며, P2R에 상응하는 서열인 RNA 분자이다.)
  6. 제5항에 있어서, (6) 식 P1R-TR-P2R의 다중 RNA 복제물을 상기 제2프라이머와 혼성화하여
    P1R-TR-P2R
    P2'
    를 형성하고 : (7) 상보적가닥을 연장시켜
    P1R-TR-P2R
    P1'-T'-P2'
    를 형성하고, 변성시켜 단일가닥 폴리누클레오티드의 제3콜렉션을 형성하고 : (8) 단일가닥 폴리누클레오티드의 제3콜렉션을 상기 제1플라이머와 혼성화시켜
    P1'-T'-P2'
    를 형성하고 : (9) 상보적가닥을 신장시켜
    PR--P1--T--P2
    PR'-P1'-T'-P2'
    를 형성하고, (10) 단계(5)를 반복하고 : (11) 임의로 단계(6) 내지 (10)을 반복함을 또다른 특징으로 하는 방법. (단계(6) 내지 (10)의 사이클에서, 각 PR-P 및 P2' 올리고누클레오티드 프라이머는 전기단계의 사이클로부터 상응하는 프라이머 또는 상응하는 프라이머와 상이한 프라이머를 나타낸다.)
  7. 제5항에 있어서, 상기 표적단일가닥 폴리누클레오티드 분자가 RNA인 방법.
  8. 제5항에 있어서, 상기 표적 단일 가닥 폴리누클레오티드 분자가 DNA인 방법.
  9. 제5항에 있어서, 연장이 상기의 연장될 서열을 역전사효소와 접촉시키는 방법인 방법.
  10. 제5항에 있어서, 상기 RNA 폴리머라제가 T7 RNA 폴리머라제인 방법.
  11. 제1항에 있어서, 상기 프라이머가 10 내지 30 누클레오티드 길이의 결합서열을 함유하는 방법.
  12. 제1항에 있어서, 상기 프라이머가 200 이하의 염기쌍에 의해 분리되는 방법.
  13. 제1항에 있어서, 상기 프로모터서열이 프라이머의 5' 말단에 있는 방법.
  14. 제1항에 있어서, 상기 표적 단일가닥 폴리누클레오티드 분자가 DNA이고, 연장이 역전사효소에 의해 수행되며, 상기 프라이머가 150염기쌍 이하로 떨어진 중간체에 존재하는 방법.
  15. 제1항에 따른 상기 표적서열의 복제수를 증식시키고 : 상기 증식된 복제물의 존재를 검출함을 특징으로 하는, 시료에 표적 폴리누클레오티드 서열의 검출방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019890700555A 1987-07-31 1989-03-30 표적 누클레오티드 서열의 선택적 증식 Expired - Lifetime KR960013577B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US8047987A 1987-07-31 1987-07-31
US080,479 1987-07-31
PCT/US1988/002601 WO1989001050A1 (en) 1987-07-31 1988-07-29 Selective amplification of target polynucleotide sequences

Publications (2)

Publication Number Publication Date
KR890701764A true KR890701764A (ko) 1989-12-21
KR960013577B1 KR960013577B1 (ko) 1996-10-09

Family

ID=22157643

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019890700555A Expired - Lifetime KR960013577B1 (ko) 1987-07-31 1989-03-30 표적 누클레오티드 서열의 선택적 증식

Country Status (13)

Country Link
US (2) US5437990A (ko)
EP (2) EP0682120B1 (ko)
JP (1) JP2774121B2 (ko)
KR (1) KR960013577B1 (ko)
AT (1) ATE135053T1 (ko)
CA (1) CA1340843C (ko)
DE (2) DE3856455T2 (ko)
DK (1) DK153289D0 (ko)
ES (1) ES2086298T3 (ko)
FI (1) FI891436L (ko)
IE (1) IE72468B1 (ko)
PT (1) PT88168B (ko)
WO (1) WO1989001050A1 (ko)

Families Citing this family (443)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5714380A (en) 1986-10-23 1998-02-03 Amoco Corporation Closed vessel for isolating target molecules and for performing amplification
CA1340843C (en) * 1987-07-31 1999-12-07 J. Lawrence Burg Selective amplification of target polynucleotide sequences
US6090591A (en) * 1987-07-31 2000-07-18 The Board Of Trustees Of The Leland Stanford Junior University Selective amplification of target polynucleotide sequences
DE68908054T2 (de) * 1988-01-21 1994-03-10 Genentech Inc Verstärkung und nachweis von nukleinsäuresequenzen.
JP2650159B2 (ja) * 1988-02-24 1997-09-03 アクゾ・ノベル・エヌ・ベー 核酸増幅方法
CA1340807C (en) * 1988-02-24 1999-11-02 Lawrence T. Malek Nucleic acid amplification process
US5130238A (en) * 1988-06-24 1992-07-14 Cangene Corporation Enhanced nucleic acid amplification process
WO1990006995A1 (en) * 1988-12-16 1990-06-28 Siska Diagnostics, Inc. Self-sustained sequence replication system
US5112734A (en) * 1989-05-26 1992-05-12 Gene-Trak Systems Target-dependent synthesis of an artificial gene for the synthesis of a replicatable rna
DK1642987T3 (da) 1989-06-02 2008-12-01 Pasteur Institut Nucleotidsekvenser dannet ud fra genomerne af retrovirusserne HIV-1, HIV-2 og SIV samt deres anvendelse til opformering af disse virale genomer samt til en vitro-diagnose af infektioner, der stammer fra disse virusser
DE69003104T2 (de) * 1989-06-12 1994-03-17 Cis Bio International Saclay Verfahren zum nachweis von spezifischen sequenzen von nukleinsäuren und ihre verwendungen.
FR2650839A1 (fr) * 1989-08-08 1991-02-15 Oris Ind Cie Procede de detection en phase homogene liquide de sequences specifiques d'acides nucleiques et ses applications
US7009041B1 (en) 1989-07-11 2006-03-07 Gen-Probe Incorporated Oligonucleotides for nucleic acid amplification and for the detection of Mycobacterium tuberculosis
US5480784A (en) * 1989-07-11 1996-01-02 Gen-Probe Incorporated Nucleic acid sequence amplification methods
US6589734B1 (en) 1989-07-11 2003-07-08 Gen-Probe Incorporated Detection of HIV
CA2020958C (en) * 1989-07-11 2005-01-11 Daniel L. Kacian Nucleic acid sequence amplification methods
US5766849A (en) * 1989-07-11 1998-06-16 Gen-Probe Incorporated Methods of amplifying nucleic acids using promoter-containing primer sequence
WO1991004340A1 (en) * 1989-09-20 1991-04-04 Cambridge Biotech Corporation In vitro, isothermal nucleic acid amplification
US5545522A (en) * 1989-09-22 1996-08-13 Van Gelder; Russell N. Process for amplifying a target polynucleotide sequence using a single primer-promoter complex
US7049102B1 (en) 1989-09-22 2006-05-23 Board Of Trustees Of Leland Stanford University Multi-gene expression profile
NO904633L (no) * 1989-11-09 1991-05-10 Molecular Diagnostics Inc Amplifikasjon av nukleinsyrer ved transkriberbar haarnaalsprobe.
US5215899A (en) * 1989-11-09 1993-06-01 Miles Inc. Nucleic acid amplification employing ligatable hairpin probe and transcription
IE65771B1 (en) * 1990-01-25 1995-11-15 Zeneca Ltd Amplification of nucleotide sequences using vectorette units
GB9002625D0 (en) * 1990-02-06 1990-04-04 Univ Singapore Human leukocyte antigen typing
US5194370A (en) * 1990-05-16 1993-03-16 Life Technologies, Inc. Promoter ligation activated transcription amplification of nucleic acid sequences
US5707796A (en) * 1990-06-11 1998-01-13 Nexstar Pharmaceuticals, Inc. Method for selecting nucleic acids on the basis of structure
FR2664290B1 (fr) * 1990-07-05 1993-01-29 Centre Nat Rech Scient Sondes genetiques specifiques de toxoplasma gondii, et leur utilisation pour la detection in vitro de toxoplasma gondii et pour le typage des souches toxoplasmiques.
US5162209A (en) * 1991-01-18 1992-11-10 Beth Israel Hospital Association Synthesis of full-length, double-stranded dna from a single-stranded linear dna template
WO1992020820A1 (en) * 1991-05-15 1992-11-26 Vanderbilt University Method to determine metastatic potential of tumor cells
DE4129653A1 (de) * 1991-09-06 1993-03-11 Boehringer Mannheim Gmbh Verfahren zum nachweis aehnlicher nukleinsaeuren
AU2663292A (en) * 1991-09-10 1993-04-05 Jack D. Love Dna/rna target and signal amplification
DE69214243T2 (de) * 1991-09-23 1997-02-06 Pfizer Verfahren für die Detektion von spezifische mRNS und DNS in Zellen
DE4132133A1 (de) * 1991-09-26 1993-04-01 Boehringer Mannheim Gmbh Verfahren zur spezifischen herstellung von ribonukleinsaeuren
DE4213029A1 (de) * 1991-09-26 1993-04-01 Boehringer Mannheim Gmbh Verfahren zur spezifischen vervielfaeltigung von nukleins aeuresequenzen
US5981179A (en) * 1991-11-14 1999-11-09 Digene Diagnostics, Inc. Continuous amplification reaction
US6100099A (en) 1994-09-06 2000-08-08 Abbott Laboratories Test strip having a diagonal array of capture spots
KR100249110B1 (ko) * 1992-05-06 2000-04-01 다니엘 엘. 캐시앙 핵산 서열 증폭 방법, 이에 이용되는 조성물 및 키트
US5843640A (en) * 1992-06-19 1998-12-01 Northwestern University Method of simultaneously detecting amplified nucleic acid sequences and cellular antigens in cells
KR100189229B1 (ko) * 1993-07-23 1999-06-01 다니엘 엘. 캐시앙, 헨리 엘. 노르호프 핵산 증폭을 강화하는 방법
FR2708288B1 (fr) 1993-07-26 1995-09-01 Bio Merieux Procédé d'amplification d'acides nucléiques par transcription utilisant le déplacement, réactifs et nécessaire pour la mise en Óoeuvre de ce procédé.
WO1995015399A1 (fr) 1993-12-01 1995-06-08 Toyo Boseki Kabushiki Kaisha Procede d'amplification et de detection d'une sequence nucleotidique au moyen d'enzymes thermostables
KR100230718B1 (ko) * 1994-03-16 1999-11-15 다니엘 엘. 캐시앙, 헨리 엘. 노르호프 등온 가닥 변위 핵산 증폭법
FR2724934B1 (fr) * 1994-09-26 1997-01-24 Bio Merieux Oligonucleotide chimere et son utilisation dans l'obtention de transcrits d'un acide nucleique
US5514551A (en) * 1994-10-14 1996-05-07 Gen-Probe Incorporated Compositions for the detection of Chlamydia trachomatis
US5652126A (en) * 1995-06-07 1997-07-29 Gen-Probe Incorporated Use of restriction endonuclease sequences for cleaving phosphorothioate oligonucleotides
US5916777A (en) * 1995-06-07 1999-06-29 Gen-Probe Incorporated Enzymatic synthesis of oligonucleotides using 3'-ribonucleotide primers
US5932450A (en) * 1995-06-07 1999-08-03 Gen-Probe Incorporated Enzymatic synthesis of oligonucleotides using digestible templates
US5739311A (en) * 1995-06-07 1998-04-14 Gen-Probe Incorporated Enzymatic synthesis of phosphorothioate oligonucleotides using restriction endonucleases
US6436638B1 (en) 1996-05-09 2002-08-20 Metropolitan Water District Of Southern California Cryptosporidium detection method
US5770368A (en) * 1996-05-09 1998-06-23 Metropolitan Water District Of Southern California Cryptosporidium detection method
CA2255624C (en) 1996-05-22 2007-09-04 Gen-Probe Incorporated Compositions and methods for the detection of mycobacterium kansasii
DK0912767T3 (da) * 1996-07-16 2006-10-30 Gen Probe Inc Fremgangsmåde til sporing og forstærkning af nukleinsyresekvenser ved anvendelse af modificerede oligonukleotider med foröget targetspecifikt TM
US7070925B1 (en) * 1996-07-16 2006-07-04 Gen-Probe Incorporated Method for determining the presence of an RNA analyte in a sample using a modified oligonucleotide probe
JPH1028585A (ja) * 1996-07-16 1998-02-03 Toyobo Co Ltd 耐熱性リボヌクレアーゼｈを用いる核酸増幅法
US5838267A (en) * 1996-10-09 1998-11-17 Ericsson, Inc. Method and apparatus for encoding and decoding digital information
US6025133A (en) * 1996-12-30 2000-02-15 Gen-Probe Incorporated Promoter-sequestered oligonucleoside and method of use
JP3968810B2 (ja) * 1997-01-24 2007-08-29 東ソー株式会社 核酸配列分析方法
WO1998044151A1 (en) 1997-04-01 1998-10-08 Glaxo Group Limited Method of nucleic acid amplification
US6429007B1 (en) * 1997-05-02 2002-08-06 BIOMéRIEUX, INC. Nucleic acid amplification reaction station for disposable test devices
AU738708B2 (en) 1997-05-02 2001-09-27 Gen-Probe Incorporated Two-step hybridization and capture of a polynucleotide
US6558901B1 (en) * 1997-05-02 2003-05-06 Biomerieux Vitek Nucleic acid assays
IL124275A (en) * 1997-05-02 2002-03-10 Bio Merieux Vitek Inc A method to produce sequences of nucleic acids
US5786182A (en) * 1997-05-02 1998-07-28 Biomerieux Vitek, Inc. Dual chamber disposable reaction vessel for amplification reactions, reaction processing station therefor, and methods of use
US6410275B1 (en) 1997-05-02 2002-06-25 Biomerieux, Inc. Disposable test devices for performing nucleic acid amplification reactions
US6534273B2 (en) 1997-05-02 2003-03-18 Gen-Probe Incorporated Two-step hybridization and capture of a polynucleotide
US5989499A (en) * 1997-05-02 1999-11-23 Biomerieux, Inc. Dual chamber disposable reaction vessel for amplification reactions
US5932451A (en) * 1997-11-19 1999-08-03 Incyte Pharmaceuticals, Inc. Method for unbiased mRNA amplification
DK1614475T3 (da) 1998-05-01 2007-09-17 Gen Probe Inc Indretning til omröring af væskeindholdet i en beholder
AR021833A1 (es) 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
US6153411A (en) * 1998-10-30 2000-11-28 American Water Works Company, Inc. Methods and kits for detection of Cryptosporidium parvum using immunomagnetic separation and amplification
US6582906B1 (en) 1999-04-05 2003-06-24 Affymetrix, Inc. Proportional amplification of nucleic acids
US6132997A (en) * 1999-05-28 2000-10-17 Agilent Technologies Method for linear mRNA amplification
US6864050B2 (en) * 1999-07-30 2005-03-08 Affymetrix, Inc. Single-phase amplification of nucleic acids
AT410218B (de) 1999-08-20 2003-03-25 Baxter Ag Verfahren zur herstellung eines qualitätsgesicherten pools biologischer proben
CN102586228A (zh) 1999-09-13 2012-07-18 纽亘技术公司 用于多核苷酸序列线性等温扩增的方法及组合物
US6692918B2 (en) * 1999-09-13 2004-02-17 Nugen Technologies, Inc. Methods and compositions for linear isothermal amplification of polynucleotide sequences
WO2001038572A1 (fr) * 1999-11-19 2001-05-31 Takara Bio Inc. Methode d'amplification d'acides nucleiques
US6582938B1 (en) 2001-05-11 2003-06-24 Affymetrix, Inc. Amplification of nucleic acids
US6794138B1 (en) 1999-12-16 2004-09-21 Affymetrix, Inc. Methods of small sample amplification
US6489114B2 (en) 1999-12-17 2002-12-03 Bio Merieux Process for labeling a ribonucleic acid, and labeled RNA fragments which are obtained thereby
US6902891B2 (en) 1999-12-17 2005-06-07 Bio Merieux Process for labeling a nucleic acid
AU2001271595A1 (en) * 2000-06-26 2002-01-08 Nugen Technologies, Inc. Methods and compositions for transcription-based nucleic acid amplification
US7846733B2 (en) 2000-06-26 2010-12-07 Nugen Technologies, Inc. Methods and compositions for transcription-based nucleic acid amplification
CA2419649C (en) 2000-09-01 2011-03-08 Gen-Probe Incorporated Amplification of hiv-1 sequences for detection of sequences associated with drug-resistance mutations
EP1399585B1 (en) 2000-10-23 2008-02-20 Gen-Probe Incorporated Compositions and methods for detecting human immunodeficiency virus 2 (hiv-2)
AR031640A1 (es) 2000-12-08 2003-09-24 Applied Research Systems Amplificacion isotermica de acidos nucleicos en un soporte solido
US6858413B2 (en) 2000-12-13 2005-02-22 Nugen Technologies, Inc. Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof
US6794141B2 (en) 2000-12-22 2004-09-21 Arcturus Bioscience, Inc. Nucleic acid amplification
US6867021B2 (en) 2001-02-20 2005-03-15 Board Of Trustees Of Michigan State University Multiplex RT-PCR/PCR for simultaneous detection of bovine coronavirus, bovine rotavirus, Cryptosporidium parvum, and Escherichia coli
AU2002252279B2 (en) 2001-03-09 2005-05-12 Nugen Technologies, Inc. Methods and compositions for amplification of RNA sequences
JP2005508135A (ja) 2001-03-09 2005-03-31 ニューゲン テクノロジーズ， インコーポレイテッド Ｒｎａ配列の増幅のための方法および組成物
WO2004059289A2 (en) * 2001-05-22 2004-07-15 Epicentre Technologies Target-dependent transcription using deletion mutants of n4 rna polymerase
US8137911B2 (en) * 2001-05-22 2012-03-20 Cellscript, Inc. Preparation and use of single-stranded transcription substrates for synthesis of transcription products corresponding to target sequences
US6872529B2 (en) * 2001-07-25 2005-03-29 Affymetrix, Inc. Complexity management of genomic DNA
US7297778B2 (en) * 2001-07-25 2007-11-20 Affymetrix, Inc. Complexity management of genomic DNA
TWI335938B (en) * 2001-08-15 2011-01-11 Rna replication and amplification
US7045319B2 (en) 2001-10-30 2006-05-16 Ribomed Biotechnologies, Inc. Molecular detection systems utilizing reiterative oligonucleotide synthesis
KR100446621B1 (ko) * 2001-12-18 2004-09-07 삼성전자주식회사 다중 중합효소 연쇄반응에 의한 ｍｏｄｙ３유전자의 증폭을 위한 프라이머 세트
CN1281324C (zh) * 2001-12-19 2006-10-25 阿菲梅特里克斯公司 阵列板和阵列板的构建方法
EP1485503A4 (en) * 2002-03-15 2005-12-28 Arcturus Bioscience Inc IMPROVED NUCLEIC ACID AMPLIFICATION
US7498171B2 (en) 2002-04-12 2009-03-03 Anthrogenesis Corporation Modulation of stem and progenitor cell differentiation, assays, and uses thereof
KR20050008720A (ko) 2002-05-17 2005-01-21 벡톤 디킨슨 앤드 컴퍼니 표적 핵산 서열의 분리, 증폭 및 검출을 위한 자동화 시스템
ATE519861T1 (de) 2002-06-14 2011-08-15 Gen Probe Inc Zusammensetzungen zum nachweis von hepatitis-b- virus
KR100442832B1 (ko) 2002-07-10 2004-08-02 삼성전자주식회사 다중 중합효소 연쇄반응에 의한 ｍｏｄｙ２ 유전자의증폭을 위한 프라이머 세트
US7504215B2 (en) 2002-07-12 2009-03-17 Affymetrix, Inc. Nucleic acid labeling methods
US20040009483A1 (en) * 2002-07-12 2004-01-15 Ilsley Diane D. Method of linear mRNA amplification using total RNA
US20050003369A1 (en) * 2002-10-10 2005-01-06 Affymetrix, Inc. Method for depleting specific nucleic acids from a mixture
US7115374B2 (en) 2002-10-16 2006-10-03 Gen-Probe Incorporated Compositions and methods for detecting West Nile virus
AU2003280603A1 (en) 2002-10-29 2004-05-25 Kabushiki Kaisha Dnaform Method of amplifying nucleic acid
US20040101844A1 (en) * 2002-11-21 2004-05-27 Amorese Douglas A. Methods and compositions for producing linearly amplified amounts of (+) strand RNA
WO2004048594A2 (en) 2002-11-21 2004-06-10 Epicentre Technologies Preparation and use of single-stranded transcription substrates for synthesis of transcription products corresponding to target sequences
US6852494B2 (en) 2003-01-10 2005-02-08 Linden Technologies, Inc. Nucleic acid amplification
WO2004083806A2 (en) 2003-01-22 2004-09-30 University Of South Florida Autonomous genosensor apparatus and methods for use
WO2004092418A2 (en) 2003-04-14 2004-10-28 Nugen Technologies, Inc. Global amplification using a randomly primed composite primer
US20040248102A1 (en) 2003-06-03 2004-12-09 Diane Ilsley-Tyree Methods and compositions for performing template dependent nucleic acid primer extension reactions that produce a reduced complexity product
EP2316973A1 (en) 2003-06-10 2011-05-04 The Trustees Of Boston University Detection methods for disorders of the lung
DK1709198T3 (da) 2003-11-26 2013-09-02 Advandx Inc Peptidnukleinsyreprober til analyse af visse staphylococcus-arter
ATE549421T1 (de) 2003-12-19 2012-03-15 Gen Probe Inc Zusammensetzungen, verfahren und kits zum nachweis der nukleinsäuren von hiv-1 und hiv-2
US20050147975A1 (en) * 2003-12-24 2005-07-07 Schembri Carol T. Methods and compositions for amplification of genomic DNA
TW200525026A (en) 2003-12-25 2005-08-01 Riken Method of amplifying nucleic acid and method of detecting mutated nucleic acid using the same
US20060003343A1 (en) 2004-01-23 2006-01-05 Biomerieux, Inc. Primer and probe design for efficient amplification and detection of HCV 3' non-translating region
US20050191682A1 (en) 2004-02-17 2005-09-01 Affymetrix, Inc. Methods for fragmenting DNA
WO2005083121A1 (en) * 2004-02-27 2005-09-09 Seegene, Inc. Method for amplifying members of a gene family
EP2933630A3 (en) 2004-06-07 2015-10-28 Fluidigm Corporation Optical lens system and method for microfluidic devices
WO2006007603A2 (en) * 2004-07-13 2006-01-19 Gen-Probe Incorporated Compositions and methods for detection of hepatitis a virus nucleic acid
WO2006006722A1 (ja) 2004-07-13 2006-01-19 Takeda Pharmaceutical Company Limited 細胞機能の調節方法
JPWO2006011667A1 (ja) * 2004-07-30 2008-05-01 東ソー株式会社 ヘテロ核リボヌクレオチドタンパク質Ｂ１（ｈｎＲＮＰＢ１）ｍＲＮＡの測定方法
WO2006026388A2 (en) 2004-08-27 2006-03-09 Gen-Probe Incorporated Single-primer nucleic acid amplification methods
US7713697B2 (en) * 2004-08-27 2010-05-11 Gen-Probe Incorporated Methods and kits for amplifying DNA
EP1647600A3 (en) 2004-09-17 2006-06-28 Affymetrix, Inc. (A US Entity) Methods for identifying biological samples by addition of nucleic acid bar-code tags
AU2005291899A1 (en) 2004-09-30 2006-04-13 Gen-Probe Incorporated Assay for detecting and quantifying HIV-1
EP1645640B1 (en) 2004-10-05 2013-08-21 Affymetrix, Inc. Method for detecting chromosomal translocations
US7682782B2 (en) 2004-10-29 2010-03-23 Affymetrix, Inc. System, method, and product for multiple wavelength detection using single source excitation
CA2524964A1 (en) 2004-10-29 2006-04-29 Affymetrix, Inc. Automated method of manufacturing polymer arrays
JP5192238B2 (ja) * 2004-11-09 2013-05-08 ジェン−プローブ・インコーポレーテッド Ａ群連鎖球菌属を検出するための組成物及び方法
US7579153B2 (en) * 2005-01-25 2009-08-25 Population Genetics Technologies, Ltd. Isothermal DNA amplification
DK1859330T3 (da) 2005-01-28 2012-10-15 Univ Duke Apparater og fremgangsmåder til håndtering af små dråber på et trykt kredsløbskort
CA2592179C (en) * 2005-02-07 2012-04-03 Gen-Probe Incorporated Compositions and methods for detecting group b streptococci
JP4819064B2 (ja) * 2005-02-18 2011-11-16 ジェン−プロウブ インコーポレイテッド アルカリショックを取り入れたサンプル調製法
US9127305B2 (en) * 2005-02-28 2015-09-08 Bioquest, Inc. Methods for performing direct enzymatic reactions involving nucleic acid molecules
US20060223071A1 (en) * 2005-04-01 2006-10-05 Wisniewski Michele E Methods, compositions, and kits for detecting nucleic acids in a single vessel
EP2360278A1 (en) 2005-04-14 2011-08-24 Trustees Of Boston University Diagnostic for lung disorders using class prediction
WO2006121773A2 (en) 2005-05-06 2006-11-16 Gen-Probe Incorporated Compositions and assays to detect influenza virus a and b nucleic acids
JP2008539766A (ja) 2005-05-17 2008-11-20 オズジーン プロプライアタリー リミテッド 連続的クローニング系
US7550264B2 (en) 2005-06-10 2009-06-23 Datascope Investment Corporation Methods and kits for sense RNA synthesis
GB0514935D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Methods for sequencing a polynucleotide template
GB0514910D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Method for sequencing a polynucleotide template
JP2009506788A (ja) * 2005-09-06 2009-02-19 ジェン−プローブ・インコーポレーテッド 核酸の等温増幅のための方法、組成物及びキット
US7939258B2 (en) 2005-09-07 2011-05-10 Nugen Technologies, Inc. Nucleic acid amplification procedure using RNA and DNA composite primers
CA2625414A1 (en) 2005-10-17 2007-04-26 Gen-Probe Incorporated Compositions and methods to detect legionella pneumophila nucleic acid
EP2273404B1 (en) 2005-11-14 2013-10-09 Gen-Probe Incorporated Computer-readable medium for parametric calibration method
KR101321658B1 (ko) * 2006-01-18 2013-10-23 아고스 쎄라퓨틱스, 인코포레이티드 폐쇄 용기에서 샘플을 처리하기 위한 시스템 및 방법, 및관련 소자
US7368265B2 (en) * 2006-01-23 2008-05-06 Compass Genetics, Llc Selective genome amplification
CA2641851A1 (en) 2006-02-08 2007-08-16 Eric Hans Vermaas Method for sequencing a polynucleotide template
US20110143344A1 (en) * 2006-03-01 2011-06-16 The Washington University Genetic polymorphisms and substance dependence
US20090176878A1 (en) * 2007-10-05 2009-07-09 Washington University In St. Louis Genetic polymorphisms and substance dependence
US20090061454A1 (en) 2006-03-09 2009-03-05 Brody Jerome S Diagnostic and prognostic methods for lung disorders using gene expression profiles from nose epithelial cells
ATE514794T1 (de) * 2006-05-12 2011-07-15 Gen Probe Inc Zusammensetzungen und verfahren für den nachweis von enterokokken-nukleinsäuren
US20120196827A1 (en) 2006-06-12 2012-08-02 Oncomethylome Sciences S.A. Methylation markers for early detection and prognosis of colon cancers
EP2069536B1 (en) 2006-08-01 2014-11-05 Gen-Probe Incorporated Methods of nonspecific target capture of nucleic acids
JP5244103B2 (ja) 2006-08-09 2013-07-24 ホームステッド クリニカル コーポレイション 器官特異的蛋白質およびその使用方法
US7754429B2 (en) 2006-10-06 2010-07-13 Illumina Cambridge Limited Method for pair-wise sequencing a plurity of target polynucleotides
US9845494B2 (en) 2006-10-18 2017-12-19 Affymetrix, Inc. Enzymatic methods for genotyping on arrays
US20090275632A1 (en) * 2006-10-27 2009-11-05 Oncomethylome Sciences Sa Methods of diagnosis and treatment
EP2092074A4 (en) 2006-11-02 2010-06-09 Univ Yale EVALUATION OF THE EFFECTIVENESS OF EGGS
US8293684B2 (en) * 2006-11-29 2012-10-23 Exiqon Locked nucleic acid reagents for labelling nucleic acids
EP2121956B1 (en) 2006-12-21 2016-08-17 Gen-Probe Incorporated Methods and compositions for nucleic acid amplification
GB0701253D0 (en) 2007-01-23 2007-02-28 Diagnostics For The Real World Nucleic acid amplification and testing
WO2008093098A2 (en) 2007-02-02 2008-08-07 Illumina Cambridge Limited Methods for indexing samples and sequencing multiple nucleotide templates
US8183359B2 (en) * 2007-03-01 2012-05-22 Gen-Probe Incorporated Kits for amplifying DNA
WO2009006438A2 (en) 2007-06-29 2009-01-08 Epicentre Technologies Corporation Copy dna and sense rna
CA2692800A1 (en) * 2007-07-09 2009-01-15 Christopher John Stanley Detection of micro-organisms based on their nad-dependent dna ligase activity
ES2453108T3 (es) 2007-08-06 2014-04-04 Orion Genomics, Llc Nuevos polimorfismos de un único nucleótido y combinaciones de polimorfismos nuevos y conocidos para determinar la expresión específica de alelo del gen IGF2
JP2009077712A (ja) 2007-09-11 2009-04-16 F Hoffmann La Roche Ag Ｂ−Ｒａｆキナーゼ阻害剤に対する感受性についての診断試験
KR20100085917A (ko) 2007-09-17 2010-07-29 온코메틸롬 사이언시즈 에스에이 개선된 ｍａｇｅ-ａ 발현 검출법
JP5697448B2 (ja) 2007-09-17 2015-04-08 エムディーエックスヘルス エスエー 膀胱癌の検出のための新規なマーカー
WO2009059317A2 (en) 2007-11-01 2009-05-07 University Of Iowa Research Foundation Predicting amd with snps within or near c2, factor b, plekha1, htra1, prelp, or loc387715
US7595164B2 (en) * 2007-12-26 2009-09-29 Gen-Probe Incorporated Compositions and methods to detect Candida albicans nucleic acid
WO2009089598A2 (en) * 2008-01-18 2009-07-23 Katholieke Universiteit Leuven Msmb-gene methylation based diagnosis, staging and prognosis of prostate cancer
US20090203531A1 (en) 2008-02-12 2009-08-13 Nurith Kurn Method for Archiving and Clonal Expansion
EP2677041A3 (en) 2008-02-19 2014-04-09 MDxHealth SA Detection and prognosis of lung cancer
US12060554B2 (en) 2008-03-10 2024-08-13 Illumina, Inc. Method for selecting and amplifying polynucleotides
CA2718092C (en) 2008-03-21 2019-03-19 Oncomethylome Sciences S.A. Detection and prognosis of cervical cancer
WO2009117698A2 (en) 2008-03-21 2009-09-24 Nugen Technologies, Inc. Methods of rna amplification in the presence of dna
EP2808405B1 (en) 2008-04-21 2018-06-06 Gen-Probe Incorporated Method for Detecting Chikungunya Virus
CA2724343A1 (en) 2008-05-15 2009-11-19 Ribomed Biotechnologies, Inc. Methods and reagents for detecting cpg methylation with a methyl cpg binding protein (mbp)
AU2009262870B2 (en) * 2008-05-30 2014-03-20 Gen-Probe Incorporated Compositions, kits and related methods for the detection and/or monitoring of Salmonella
WO2010009060A2 (en) 2008-07-13 2010-01-21 Ribomed Biotechnologies, Inc. Molecular beacon-based methods for detection of targets using abscription
WO2010011506A2 (en) * 2008-07-23 2010-01-28 The Washington University Risk factors and a therapeutic target for neurodegenerative disorders
GB0814570D0 (en) 2008-08-08 2008-09-17 Diagnostics For The Real World Isolation of nucleic acid
CA2679750A1 (en) 2008-09-23 2010-03-23 Nexus Dx, Inc. Methods for detecting nucleic acids in a sample
US8728764B2 (en) 2008-10-02 2014-05-20 Illumina Cambridge Limited Nucleic acid sample enrichment for sequencing applications
EP2394170B1 (en) 2009-02-03 2015-04-01 MDxHealth SA Methods of detecting colorectal cancer
CN104878086A (zh) 2009-02-11 2015-09-02 卡里斯Mpi公司 肿瘤的分子谱分析
WO2010093820A2 (en) 2009-02-11 2010-08-19 Orion Genomics Llc Combinations of polymorphisms for determining allele-specific expression of igf2
AU2010217928B2 (en) 2009-02-26 2013-06-06 Gen-Probe Incorporated Assay for detection of human parvovirus nucleic acid
EP2406393B1 (en) 2009-03-13 2016-05-04 MDxHealth SA Novel markers for bladder cancer detection
KR20120007002A (ko) 2009-03-15 2012-01-19 리보메드 바이오테크놀로지스, 인코퍼레이티드 압스크립션 기반 분자 검출
AU2010225125A1 (en) 2009-03-17 2011-10-20 Glaxosmithkline Biologicals S.A. Improved detection of gene expression
WO2010126913A1 (en) 2009-04-27 2010-11-04 Gen-Probe Incorporated Methods and kits for use in the selective amplification of target sequences
CN102459649A (zh) * 2009-05-29 2012-05-16 肺结核诊断公司 先进的病原体检测和筛选
EP3450573B1 (en) 2009-06-23 2022-01-26 Gen-Probe Incorporated Compositions and methods for detecting nucleic acid from mollicutes
US8512955B2 (en) 2009-07-01 2013-08-20 Gen-Probe Incorporated Methods and compositions for nucleic acid amplification
US20110059453A1 (en) * 2009-08-23 2011-03-10 Affymetrix, Inc. Poly(A) Tail Length Measurement by PCR
EP3133169B1 (en) 2009-08-25 2019-10-16 Illumina, Inc. Methods for selecting and amplifying polynucleotides
CN102858995B (zh) 2009-09-10 2016-10-26 森特瑞隆技术控股公司 靶向测序方法
US10174368B2 (en) 2009-09-10 2019-01-08 Centrillion Technology Holdings Corporation Methods and systems for sequencing long nucleic acids
US8182994B2 (en) 2009-09-15 2012-05-22 Illumina Cambridge Limited Centroid markers for image analysis of high denisty clusters in complex polynucleotide sequencing
WO2011036173A1 (en) 2009-09-24 2011-03-31 Oncomethylome Sciences S.A. Detection and prognosis of cervical cancer
EP2494077A4 (en) 2009-10-27 2013-08-21 Caris Mpi Inc MOLECULAR PROFILING FOR PERSONALIZED MEDICINE
WO2011066476A1 (en) * 2009-11-25 2011-06-03 Quantalife, Inc. Methods and compositions for detecting genetic material
US8501122B2 (en) 2009-12-08 2013-08-06 Affymetrix, Inc. Manufacturing and processing polymer arrays
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
US9315857B2 (en) 2009-12-15 2016-04-19 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse label-tags
WO2011078439A1 (en) * 2009-12-24 2011-06-30 Seegene, Inc. Real-time multiplexing detection of target nucleic acid sequences with elimination of false signals
US9074246B2 (en) * 2010-01-25 2015-07-07 Rd Biosciences, Inc. Self-folding amplification of target nucleic acid
WO2011103274A1 (en) 2010-02-17 2011-08-25 Gen-Probe Incorporated Compositions and methods to detect atopobium vaginae nucleic acid
WO2011128096A1 (en) 2010-04-16 2011-10-20 Roche Diagnostics Gmbh Polymorphism markers for predicting response to interleukin-6 receptor-inhibiting monoclonal antibody drug treatment
EP3037555B1 (en) 2010-04-21 2019-07-24 Gen-Probe Incorporated Compositions, methods and kits to detect herpes simplex virus nucleic acids
WO2011135058A2 (en) 2010-04-30 2011-11-03 Mdxhealth Sa Methods for detecting epigenetic modifications
KR101223660B1 (ko) 2010-05-20 2013-01-17 광주과학기술원 ＨＩＦ－２α 억제제를 유효성분으로 포함하는 관절염 예방 또는 치료용 약제학적 조성물
KR101176139B1 (ko) 2010-05-20 2012-08-22 광주과학기술원 관절염 동물모델로서 연골 특이적 ＨＩＦ?２α 형질전환 마우스 및 이의 용도
CA2801468C (en) 2010-06-04 2018-09-04 Chronix Biomedical Prostate cancer associated circulating nucleic acid biomarkers
EP2593569B1 (en) 2010-07-12 2018-01-03 Gen-Probe Incorporated Compositions and assays to detect seasonal h1 influenza a virus nucleic acids
EP2596135A2 (en) 2010-07-23 2013-05-29 Beckman Coulter, Inc. System and method including analytical units
US10533223B2 (en) 2010-08-06 2020-01-14 Ariosa Diagnostics, Inc. Detection of target nucleic acids using hybridization
US20120034603A1 (en) 2010-08-06 2012-02-09 Tandem Diagnostics, Inc. Ligation-based detection of genetic variants
US20130040375A1 (en) 2011-08-08 2013-02-14 Tandem Diagnotics, Inc. Assay systems for genetic analysis
US8700338B2 (en) 2011-01-25 2014-04-15 Ariosa Diagnosis, Inc. Risk calculation for evaluation of fetal aneuploidy
US20140342940A1 (en) 2011-01-25 2014-11-20 Ariosa Diagnostics, Inc. Detection of Target Nucleic Acids using Hybridization
US10167508B2 (en) 2010-08-06 2019-01-01 Ariosa Diagnostics, Inc. Detection of genetic abnormalities
US11031095B2 (en) 2010-08-06 2021-06-08 Ariosa Diagnostics, Inc. Assay systems for determination of fetal copy number variation
WO2012019168A2 (en) 2010-08-06 2012-02-09 Moderna Therapeutics, Inc. Engineered nucleic acids and methods of use thereof
US11203786B2 (en) 2010-08-06 2021-12-21 Ariosa Diagnostics, Inc. Detection of target nucleic acids using hybridization
US20130261003A1 (en) 2010-08-06 2013-10-03 Ariosa Diagnostics, In. Ligation-based detection of genetic variants
US20120058481A1 (en) 2010-08-20 2012-03-08 Life Technologies Corporation Quantitative Real Time PCR Assay Using FRET Dual-Labeled Primers
WO2012030856A2 (en) 2010-08-30 2012-03-08 Gen-Probe Incorporated Compositions, methods and reaction mixtures for the detection of xenotropic murine leukemia virus-related virus
US20140004510A1 (en) 2010-09-24 2014-01-02 Massachusetts Eye And Ear Infirmary Methods and compositions for prognosing and/or detecting age-related macular degeneration
SG10201508149TA (en) 2010-10-01 2015-10-29 Moderna Therapeutics Inc Engineered nucleic acids and methods of use thereof
GB2497495B (en) 2010-10-04 2018-06-06 Gen Probe Prodesse Inc Compositions, methods and kits to detect adenovirus nucleic acids
US10131947B2 (en) 2011-01-25 2018-11-20 Ariosa Diagnostics, Inc. Noninvasive detection of fetal aneuploidy in egg donor pregnancies
US11270781B2 (en) 2011-01-25 2022-03-08 Ariosa Diagnostics, Inc. Statistical analysis for non-invasive sex chromosome aneuploidy determination
US20120190021A1 (en) 2011-01-25 2012-07-26 Aria Diagnostics, Inc. Detection of genetic abnormalities
US9994897B2 (en) 2013-03-08 2018-06-12 Ariosa Diagnostics, Inc. Non-invasive fetal sex determination
US8756020B2 (en) 2011-01-25 2014-06-17 Ariosa Diagnostics, Inc. Enhanced risk probabilities using biomolecule estimations
AU2012214312A1 (en) 2011-02-09 2013-08-22 Bio-Rad Laboratories, Inc. Analysis of nucleic acids
EP3062108B1 (en) 2011-02-24 2019-06-19 Hill's Pet Nutrition, Inc. Methods for diagnosing kidney disorders in a feline
WO2012118745A1 (en) 2011-02-28 2012-09-07 Arnold Oliphant Assay systems for detection of aneuploidy and sex determination
US9512467B2 (en) 2011-03-10 2016-12-06 Gen-Probe Incorporated Methods and compositions for the selection and optimization of oligonucleotide tag sequences
DE12722942T1 (de) 2011-03-31 2021-09-30 Modernatx, Inc. Freisetzung und formulierung von manipulierten nukleinsäuren
US20120252682A1 (en) 2011-04-01 2012-10-04 Maples Corporate Services Limited Methods and systems for sequencing nucleic acids
US9657352B2 (en) 2011-04-25 2017-05-23 Gen-Probe Incorporated Compositions and methods for detecting BV-associated bacterial nucleic acid
EP2721175B1 (en) 2011-06-15 2015-09-16 Hill's Pet Nutrition, Inc. Compositions and methods for diagnosing and monitoring hyperthyroidism in a feline
WO2012177906A1 (en) 2011-06-21 2012-12-27 Alnylam Pharmaceuticals, Inc. Assays and methods for determining activity of a therapeutic agent in a subject
JP6150795B2 (ja) 2011-07-15 2017-06-28 ジェン−プロウブ インコーポレイテッド シングルプレックス又はマルチプレックスアッセイにおいてヒトパルボウイルス核酸を検出及びａ型肝炎ウイルス核酸を検出するための組成物及び方法
WO2013024173A1 (en) 2011-08-17 2013-02-21 Technische Universität München Computer implemented method for identifying regulatory regions or regulatory variations
CA2845381A1 (en) 2011-08-31 2013-03-07 F. Hoffmann-La Roche Ag Responsiveness to angiogenesis inhibitors
EP2751280B1 (en) 2011-08-31 2016-06-29 F.Hoffmann-La Roche Ag Method for predicting risk of hypertension associated with anti-angiogenesis therapy
US8712697B2 (en) 2011-09-07 2014-04-29 Ariosa Diagnostics, Inc. Determination of copy number variations using binomial probability calculations
WO2013036928A1 (en) 2011-09-08 2013-03-14 Gen-Probe Incorporated Compositions and methods for detecting bv-associated bacterial nucleic acid
US9464124B2 (en) 2011-09-12 2016-10-11 Moderna Therapeutics, Inc. Engineered nucleic acids and methods of use thereof
MX2014002990A (es) 2011-09-19 2014-05-21 Genentech Inc Tratamientos combinados que comprenden antagonistas de c-met y antagonistas de b-raf.
GB201206209D0 (en) 2012-04-06 2012-05-23 Univ Leuven Kath Marker gene based diagnosis, staging and prognosis of prostate caner
DK3682905T3 (da) 2011-10-03 2022-02-28 Modernatx Inc Modificerede nukleosider, nukleotider og nukleinsyrer og anvendelser deraf
FR2981088B1 (fr) 2011-10-06 2013-11-29 Biomerieux Sa Arn polymerases mutees
EP2768985B1 (en) 2011-10-21 2019-03-20 Chronix Biomedical Colorectal cancer associated circulating nucleic acid biomarkers
JP6320926B2 (ja) 2011-11-07 2018-05-09 ベックマン コールター， インコーポレイテッド 遠心分離機システムおよびワークフロー
BR112014011035A2 (pt) 2011-11-07 2017-06-13 Beckman Coulter, Inc. sistema de aliquotagem e fluxo de trabalho
JP2014532881A (ja) 2011-11-07 2014-12-08 ベックマン コールター， インコーポレイテッド 標本輸送システムのための磁気制動
KR20140092378A (ko) 2011-11-07 2014-07-23 베크만 컬터, 인코포레이티드 샘플을 처리하기 위한 시스템 및 방법
US9446418B2 (en) 2011-11-07 2016-09-20 Beckman Coulter, Inc. Robotic arm
JP6062449B2 (ja) 2011-11-07 2017-01-18 ベックマン コールター， インコーポレイテッド 標本コンテナ検出
MX2014006186A (es) 2011-11-23 2014-07-14 Hoffmann La Roche Capacidad de respuesta a los inhibidores de la angiogenesis.
DE102011120550B4 (de) 2011-12-05 2013-11-07 Gen-Probe Prodesse, Inc. Zusammensetzungen, Verfahren und Kits zur Detektion von Adenovirusnukleinsäuren
CN110201187A (zh) 2011-12-16 2019-09-06 现代泰克斯公司 经修饰的核苷、核苷酸和核酸组合物
JP6189857B2 (ja) 2011-12-19 2017-08-30 ヒルズ・ペット・ニュートリシャン・インコーポレーテッド 伴侶動物において甲状腺機能亢進症を診断および治療するための組成物および方法
US9115394B2 (en) 2011-12-22 2015-08-25 Roche Molecular Systems, Inc. Methods and reagents for reducing non-specific amplification
EP3453770B1 (en) 2012-02-24 2021-04-21 Gen-Probe Prodesse, Inc. Detection of shiga toxin genes in bacteria
EP2820174B1 (en) 2012-02-27 2019-12-25 The University of North Carolina at Chapel Hill Methods and uses for molecular tags
EP2820158B1 (en) 2012-02-27 2018-01-10 Cellular Research, Inc. Compositions and kits for molecular counting
US9254311B2 (en) 2012-04-02 2016-02-09 Moderna Therapeutics, Inc. Modified polynucleotides for the production of proteins
EP3978030A1 (en) 2012-04-02 2022-04-06 ModernaTX, Inc. Modified polynucleotides for the production of proteins associated with human disease
US9283287B2 (en) 2012-04-02 2016-03-15 Moderna Therapeutics, Inc. Modified polynucleotides for the production of nuclear proteins
US9572897B2 (en) 2012-04-02 2017-02-21 Modernatx, Inc. Modified polynucleotides for the production of cytoplasmic and cytoskeletal proteins
AU2013205110B2 (en) 2012-04-24 2016-10-13 Gen-Probe Incorporated Compositions, Methods and Kits to Detect Herpes Simplex Virus Nucleic Acids
US10289800B2 (en) 2012-05-21 2019-05-14 Ariosa Diagnostics, Inc. Processes for calculating phased fetal genomic sequences
GB201208926D0 (en) 2012-05-21 2012-07-04 Vela Operations Pte Ltd Extraction control for DNA
CA2874413A1 (en) 2012-05-21 2013-11-28 The Scripps Research Institute Methods of sample preparation
AU2013205064B2 (en) 2012-06-04 2015-07-30 Gen-Probe Incorporated Compositions and Methods for Amplifying and Characterizing HCV Nucleic Acid
AU2013205087B2 (en) 2012-07-13 2016-03-03 Gen-Probe Incorporated Method for detecting a minority genotype
JP2015522293A (ja) 2012-07-19 2015-08-06 アリオサ ダイアグノスティックス インコーポレイテッドＡｒｉｏｓａ Ｄｉａｇｎｏｓｔｉｃｓ，Ｉｎｃ． 多重化連続ライゲーションに基づく遺伝子変異体の検出
WO2014018885A2 (en) 2012-07-27 2014-01-30 Gen-Probe Incorporated Dual reference calibration method and system for quantifying polynucleotides
AU2013202808B2 (en) 2012-07-31 2014-11-13 Gen-Probe Incorporated System and method for performing multiplex thermal melt analysis
JP6464086B2 (ja) 2012-08-30 2019-02-06 ジェン−プローブ・インコーポレーテッド 多相核酸増幅
MX2015004191A (es) 2012-10-04 2016-04-04 Univ Leland Stanford Junior Metodos y reactivos para la deteccion, cuantificacion y serotipado de virus del dengue.
AU2013205122B2 (en) 2012-10-11 2016-11-10 Gen-Probe Incorporated Compositions and Methods for Detecting Human Papillomavirus Nucleic Acid
ES2921623T3 (es) 2012-11-26 2022-08-30 Modernatx Inc ARN modificado terminalmente
AU2013205090B2 (en) 2012-12-07 2016-07-28 Gen-Probe Incorporated Compositions and Methods for Detecting Gastrointestinal Pathogen Nucleic Acid
US10077475B2 (en) 2013-01-24 2018-09-18 California Institute Of Technology FRET-based analytes detection and related methods and systems
US20140213471A1 (en) 2013-01-24 2014-07-31 California Institute Of Technology Chromophore-based characterization and detection methods
AU2013202805B2 (en) 2013-03-14 2015-07-16 Gen-Probe Incorporated System and method for extending the capabilities of a diagnostic analyzer
WO2014152937A1 (en) 2013-03-14 2014-09-25 Ibis Biosciences, Inc. Nucleic acid control panels
EP2971090B1 (en) * 2013-03-15 2020-05-06 Gen-Probe Incorporated Calibration method, apparatus and computer program product
US8980864B2 (en) 2013-03-15 2015-03-17 Moderna Therapeutics, Inc. Compositions and methods of altering cholesterol levels
KR101403507B1 (ko) 2013-03-21 2014-06-09 주식회사 현일바이오 결핵균 및 비결핵 마이코박테리아의 선택적 검출 방법, 그리고 이를 이용한 키트
EP2978857A2 (en) 2013-03-27 2016-02-03 Sample Technologies, Inc. Recombinant phage and bacterial detection methods
KR101507505B1 (ko) 2013-04-18 2015-04-07 사회복지법인 삼성생명공익재단 제 1 형 근긴장성 이영양증의 진단 방법
PL3004388T5 (pl) 2013-05-29 2023-10-09 Chronix Biomedical Wykrywanie i oznaczanie ilościowe pozakomórkowego dna dawcy w układzie krążenia biorców przeszczepów organów
EP3663414B1 (en) 2013-06-13 2023-11-22 Roche Diagnostics GmbH Statistical analysis for non-invasive y chromosome aneuploidy determination
CA2915820A1 (en) 2013-06-19 2014-12-24 Michael Sandor Koeris Phage-based bacterial detection assay
US9547006B2 (en) 2013-08-08 2017-01-17 Institut Pasteur Correlation of disease activity with clonal expansions of human papillomavirus 16-specific CD8+ T-cells in patients with severe erosive oral lichen planus
EP3033437B1 (en) 2013-08-14 2023-11-08 Gen-Probe Incorporated Compositions and methods for detecting hev nucleic acid
EP3480321B8 (en) 2013-08-28 2021-03-10 Becton, Dickinson and Company Massively parallel single cell analysis
WO2015048744A2 (en) 2013-09-30 2015-04-02 Moderna Therapeutics, Inc. Polynucleotides encoding immune modulating polypeptides
BR112016007255A2 (pt) 2013-10-03 2017-09-12 Moderna Therapeutics Inc polinucleotídeos que codificam receptor de lipoproteína de baixa densidade
CN105745528A (zh) 2013-10-07 2016-07-06 赛卢拉研究公司 用于以数字方式对阵列上的特征进行计数的方法和系统
BR112016011046A2 (pt) 2013-11-15 2019-01-29 Centre Nat Rech Scient método para genotipagem, método para a detecção de uma infecção, kit para detectar uma infecção e método para detectar em uma amostra biológica.
GB201322034D0 (en) 2013-12-12 2014-01-29 Almac Diagnostics Ltd Prostate cancer classification
JP2017516463A (ja) 2014-03-31 2017-06-22 デバイオファーム インターナショナル エスエイ Ｆｇｆｒ融合体
AU2015315103B2 (en) 2014-09-09 2022-01-27 Igenomx International Genomics Corporation Methods and compositions for rapid nucleic acid library preparation
WO2016077366A1 (en) 2014-11-10 2016-05-19 Genentec, Inc. Therapeutic and diagnostic methods for il-33-mediated disorders
EP3242956B1 (en) 2015-01-09 2020-06-17 Gen-Probe Incorporated Methods and compositions for diagnosing bacterial vaginosis
KR101718800B1 (ko) 2015-01-21 2017-03-24 주식회사 디알나노 약물 및 ｓｉＲＮＡ의 공동―운반용 나노복합체 및 이의 용도
EP3259371B1 (en) 2015-02-19 2020-09-02 Becton, Dickinson and Company High-throughput single-cell analysis combining proteomic and genomic information
ES2836802T3 (es) 2015-02-27 2021-06-28 Becton Dickinson Co Códigos de barras moleculares espacialmente direccionables
CA2977821A1 (en) 2015-03-16 2016-09-22 Gen-Probe Incorporated Methods and compositions for detecting bacterial nucleic acid and diagnosing bacterial vaginosis
ES2934982T3 (es) 2015-03-30 2023-02-28 Becton Dickinson Co Métodos para la codificación con códigos de barras combinatorios
WO2016172373A1 (en) 2015-04-23 2016-10-27 Cellular Research, Inc. Methods and compositions for whole transcriptome amplification
JP6966681B2 (ja) 2015-04-24 2021-11-17 アティラ バイオシステムズ インコーポレイテッドAtila Biosystems Incorporated 限られたヌクレオチド組成を有するプライマーを用いた増幅
WO2016196229A1 (en) 2015-06-01 2016-12-08 Cellular Research, Inc. Methods for rna quantification
GB201510684D0 (en) 2015-06-17 2015-08-05 Almac Diagnostics Ltd Gene signatures predictive of metastatic disease
PT3385395T (pt) 2015-08-17 2020-05-06 Kura Oncology Inc Métodos de tratamento de doentes com cancro usando inibidores da farnesiltransferase
ES2745694T3 (es) 2015-09-11 2020-03-03 Cellular Res Inc Métodos y composiciones para normalización de biblioteca de ácido nucleico
KR20180071299A (ko) 2015-10-18 2018-06-27 아피메트릭스, 인코포레이티드 단일 뉴클레오타이드 다형 및 인델의 다대립유전자 유전자형분석
KR101651817B1 (ko) 2015-10-28 2016-08-29 대한민국 Ngs 라이브러리 제작용 프라이머 세트 및 이를 이용한 ngs 라이브러리 제작방법 및 키트
WO2017120216A2 (en) 2016-01-04 2017-07-13 Gen-Probe Incorporated Methods and compositions for detecting candida species
GB201605110D0 (en) 2016-03-24 2016-05-11 Mologic Ltd Detecting sepsis
WO2017184968A1 (en) 2016-04-22 2017-10-26 Kura Oncology, Inc. Methods of selecting cancer patients for treatment with farnesyltransferase inhibitors
ES2956757T3 (es) 2016-05-02 2023-12-27 Becton Dickinson Co Codificación con códigos de barras moleculares precisa
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
ES2979395T3 (es) 2016-05-26 2024-09-25 Becton Dickinson Co Métodos de ajuste del recuento de etiquetas moleculares
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
WO2017214511A2 (en) 2016-06-10 2017-12-14 Gen-Probe Incorporated Compositions and methods for detecting zika virus nucleic acid
US12168805B2 (en) 2016-08-10 2024-12-17 Institut Pasteur Methods and reagents for detecting piperaquine-resistant plasmodium falciparum malaria
CA3034924A1 (en) 2016-09-26 2018-03-29 Cellular Research, Inc. Measurement of protein expression using reagents with barcoded oligonucleotide sequences
US11447835B2 (en) 2016-10-19 2022-09-20 Gen-Probe Incorporated Compositions and methods for detecting or quantifying hepatitis C virus
BR112019009000A2 (pt) 2016-11-03 2019-07-16 Kura Oncology Inc método para tratar um carcinoma refratário, método de tratamento de scchn, método de tratamento de carcinoma de células escamosas, método de tratamento para scc
AU2017359048B2 (en) 2016-11-08 2022-07-21 Becton, Dickinson And Company Methods for expression profile classification
AU2017359047C1 (en) 2016-11-08 2024-10-24 Becton, Dickinson And Company Methods for cell label classification
US10508313B2 (en) 2016-11-21 2019-12-17 Gen-Probe Incorporated Compositions and methods for detecting or quantifying Hepatitis B virus
US10427162B2 (en) 2016-12-21 2019-10-01 Quandx Inc. Systems and methods for molecular diagnostics
KR101936799B1 (ko) 2017-01-09 2019-01-11 주식회사 엠이티라이프사이언스 구강전암의 치료용 약학 조성물 및 구강전암 또는 구강암의 예측 또는 진단 방법
WO2018132610A1 (en) 2017-01-13 2018-07-19 Cellular Research, Inc. Hydrophilic coating of fluidic channels
WO2018144240A1 (en) 2017-02-01 2018-08-09 Cellular Research, Inc. Selective amplification using blocking oligonucleotides
SG11201907594TA (en) 2017-02-21 2019-09-27 Kura Oncology Inc Methods of treating cancer with farnesyltransferase inhibitors
US10137121B2 (en) 2017-02-21 2018-11-27 Kura Oncology, Inc. Methods of treating cancer with farnesyltransferase inhibitors
EP4282986A3 (en) 2017-03-24 2024-02-28 Gen-Probe Incorporated Compositions and methods for detecting or quantifying parainfluenza virus
CA3176529C (en) 2017-03-24 2024-03-05 Gen-Probe Incorporated Compositions and methods for detection of viral pathogens in samples
US11603557B2 (en) 2017-06-02 2023-03-14 Affymetrix, Inc. Array-based methods for analysing mixed samples using differently labelled allele-specific probes
WO2018223053A1 (en) 2017-06-02 2018-12-06 Affymetrix, Inc. Array-based methods for analysing mixed samples using differently labelled allele-specific probes
US10676779B2 (en) 2017-06-05 2020-06-09 Becton, Dickinson And Company Sample indexing for single cells
WO2018226798A1 (en) 2017-06-07 2018-12-13 Gen-Probe Incorporated Detecting babesia species nucleic acid in a sample
KR101956315B1 (ko) 2017-07-19 2019-03-08 국민대학교 산학협력단 파킨슨병 바이오마커로서의 miR494 및 이를 이용한 진단키트
WO2019017680A2 (ko) 2017-07-19 2019-01-24 국민대학교 산학협력단 파킨슨병 바이오마커로서의 miRNA 및 이를 이용한 진단키트
US10806730B2 (en) 2017-08-07 2020-10-20 Kura Oncology, Inc. Methods of treating cancer with farnesyltransferase inhibitors
EP3664804A4 (en) 2017-08-07 2021-04-14 Kura Oncology, Inc. METHOD OF TREATMENT OF CANCER WITH FERN SYL TRANSFERASE INHIBITORS
EP4219766A3 (en) 2017-08-11 2023-10-11 Gen-Probe Incorporated Compositions and methods for detecting staphylococcus aureus
WO2019094578A1 (en) 2017-11-09 2019-05-16 Alnylam Pharmaceuticals Inc. Assays and methods for determining expression of the lect2 gene
WO2019099629A1 (en) 2017-11-17 2019-05-23 Gen-Probe Incorporated COMPOSITIONS AND METHODS FOR DETECTING C1orf43 NUCLEIC ACID
WO2019118735A1 (en) 2017-12-15 2019-06-20 Gen-Probe Incorporated Compositions and methods for detecting toxigenic clostridium difficile
CN111492068B (zh) 2017-12-19 2025-03-21 贝克顿迪金森公司 与寡核苷酸相关联的颗粒
FI3749781T3 (fi) 2018-02-06 2024-12-27 Gen Probe Inc Kaukopunaväriaineen koetinformulaatioita
KR102417088B1 (ko) 2018-02-09 2022-07-07 제넨테크, 인크. 비만 세포 매개 염증성 질환에 대한 치료 및 진단 방법
WO2019213294A1 (en) 2018-05-03 2019-11-07 Becton, Dickinson And Company High throughput multiomics sample analysis
CN112243461B (zh) 2018-05-03 2024-07-12 贝克顿迪金森公司 在相对的转录物末端进行分子条形码化
WO2019239394A1 (en) 2018-06-13 2019-12-19 Gen-Probe Incorporated Compositions and methods for detecting group b streptococcus nucleic acid
CA3106975A1 (en) 2018-08-01 2020-02-06 Gen-Probe Incorporated Compositions and methods for detecting nucleic acids of epstein-barr virus
EP3841223A1 (en) 2018-08-08 2021-06-30 Gen-Probe Incorporated Compositions, methods and kits for detecting mycoplasma genitalium
WO2020041414A1 (en) 2018-08-21 2020-02-27 Gen-Probe Incorporated Compositions and methods for amplifying, detecting or quantifying human cytomegalovirus
KR20210063347A (ko) 2018-08-24 2021-06-01 젠-프로브 인코포레이티드 세균성 핵산을 검출하고 세균성 질염을 진단하기 위한 조성물 및 방법
EP3856934A2 (en) 2018-09-27 2021-08-04 Gen-Probe Incorporated COMPOSITIONS AND METHODS FOR DETECTING BORDETELLA PERTUSSIS AND BORDETELLA
PARAPERTUSSIS NUCLEIC ACID
JP2022511398A (ja) 2018-10-01 2022-01-31 ベクトン・ディキンソン・アンド・カンパニー ５’転写物配列の決定
JP7542546B2 (ja) 2018-10-22 2024-08-30 ジェン－プローブ・インコーポレーテッド ヒトポリオーマウイルスｂｋウイルスを増幅、検出または定量化するための組成物および方法
AU2019372141A1 (en) 2018-11-01 2021-05-27 Kura Oncology, Inc. Methods of treating cancer with farnesyltransferase inhibitors
WO2020097315A1 (en) 2018-11-08 2020-05-14 Cellular Research, Inc. Whole transcriptome analysis of single cells using random priming
WO2020113237A1 (en) 2018-11-30 2020-06-04 Caris Mpi, Inc. Next-generation molecular profiling
WO2020123384A1 (en) 2018-12-13 2020-06-18 Cellular Research, Inc. Selective extension in single cell whole transcriptome analysis
TW202030333A (zh) 2018-12-20 2020-08-16 美商簡 探針公司 用於檢測瘧原蟲物種核酸之組成物及方法
US11371076B2 (en) 2019-01-16 2022-06-28 Becton, Dickinson And Company Polymerase chain reaction normalization through primer titration
US11661631B2 (en) 2019-01-23 2023-05-30 Becton, Dickinson And Company Oligonucleotides associated with antibodies
EP3924506A1 (en) 2019-02-14 2021-12-22 Becton Dickinson and Company Hybrid targeted and whole transcriptome amplification
GB201902458D0 (en) 2019-02-22 2019-04-10 Mologic Ltd Treatment stratification for an excerbation of inflamation
US20220142983A1 (en) 2019-03-01 2022-05-12 Kura Oncology, Inc. Methods of treating cancer with farnesyltransferase inhibitors
EP4389916A3 (en) 2019-03-22 2024-10-16 Gen-Probe Incorporated Compositions and methods for detecting group a streptococcus
WO2020205486A1 (en) 2019-03-29 2020-10-08 Kura Oncology, Inc. Methods of treating squamous cell carcinomas with farnesyltransferase inhibitors
WO2020205387A1 (en) 2019-04-01 2020-10-08 Kura Oncology, Inc. Methods of treating cancer with farnesyltransferase inhibitors
BR112021020779A8 (pt) 2019-04-17 2022-01-25 Igenomix S L Métodos aperfeiçoados para diagnóstico precoce de leiomiomas e leiomiossarcomas uterinos
US11965208B2 (en) 2019-04-19 2024-04-23 Becton, Dickinson And Company Methods of associating phenotypical data and single cell sequencing data
US20220305001A1 (en) 2019-05-02 2022-09-29 Kura Oncology, Inc. Methods of treating acute myeloid leukemia with farnesyltransferase inhibitors
WO2021003331A1 (en) 2019-07-03 2021-01-07 Gen-Probe Incorporated Oligonucleotides for use in determining the presence of trichomonas vaginalis in a sample
WO2021016239A1 (en) 2019-07-22 2021-01-28 Becton, Dickinson And Company Single cell chromatin immunoprecipitation sequencing assay
CA3152309A1 (en) 2019-08-23 2021-03-04 Gen-Probe Incorporated Compositions, methods and kits for detecting treponema pallidum
AU2020343334A1 (en) 2019-09-05 2022-04-07 Gen-Probe Incorporated Detection of Chlamydia trachomatis nucleic acid variants
GB201914538D0 (en) 2019-10-08 2019-11-20 Momentum Bioscience Ltd Microorganism capture from antimicrobial-containing solution
EP4407041A3 (en) 2019-11-08 2024-09-25 Becton Dickinson and Company Using random priming to obtain full-length v(d)j information for immune repertoire sequencing
MX2022006589A (es) 2019-12-02 2023-01-11 Caris Mpi Inc Predictor de respuesta de platino pan-cancer.
CA3163936A1 (en) 2019-12-09 2021-06-17 Gen-Probe Incorporated Quantification of polynucleotide analytes from dried samples
US11649497B2 (en) 2020-01-13 2023-05-16 Becton, Dickinson And Company Methods and compositions for quantitation of proteins and RNA
US20230227896A1 (en) 2020-01-16 2023-07-20 Dnae Diagnostics Limited Compositions, Kits and Methods for Isolating Target Polynucleotides
US12188010B2 (en) 2020-01-29 2025-01-07 Becton, Dickinson And Company Barcoded wells for spatial mapping of single cells through sequencing
CN115151810A (zh) 2020-02-25 2022-10-04 贝克顿迪金森公司 实现使用单细胞样品作为单色补偿对照的双特异性探针
CA3174532A1 (en) 2020-03-04 2021-09-10 Gen-Probe Incorporated Compositions and methods for detecting sars-cov-2 nucleic acid
US20230110203A1 (en) 2020-03-13 2023-04-13 Medimmune Limited Therapeutic methods for the treatment of subjects with risk alelles in il33
US20230220499A1 (en) 2020-05-07 2023-07-13 Grifols Diagnostic Solutions Inc. Methods and compositions for detecting sars-cov-2 nucleic acid
CN115605614A (zh) 2020-05-14 2023-01-13 贝克顿迪金森公司（Us） 用于免疫组库谱分析的引物
US12157913B2 (en) 2020-06-02 2024-12-03 Becton, Dickinson And Company Oligonucleotides and beads for 5 prime gene expression assay
EP4163397A4 (en) 2020-06-05 2024-08-28 Seegene, Inc. SAMPLE TRANSPORT KIT FOR DETECTION OF RESPIRATORY PATHOGENS AND METHODS FOR DETECTING RESPIRATORY PATHOGENS USING SAME
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
WO2022016153A1 (en) 2020-07-17 2022-01-20 Gen-Probe Incorporated Detection of macrolide-resistant mycoplasma genitalium
US11739443B2 (en) 2020-11-20 2023-08-29 Becton, Dickinson And Company Profiling of highly expressed and lowly expressed proteins
EP4337797A1 (en) 2021-05-14 2024-03-20 Gen-Probe Incorporated Compositions and methods for detecting human adenovirus nucleic acid
JP2024524334A (ja) 2021-07-01 2024-07-05 ジェン－プローブ・インコーポレーテッド 核酸増幅のための酵素製剤および反応混合物
GB202110479D0 (en) 2021-07-21 2021-09-01 Dnae Diagnostics Ltd Compositions, kits and methods for sequencing target polynucleotides
CA3226451A1 (en) 2021-07-21 2023-01-26 Sam Reed Method and system comprising a cartridge for sequencing target polynucleotides
GB202110485D0 (en) 2021-07-21 2021-09-01 Dnae Diagnostics Ltd Compositions, kits and methods for sequencing target polynucleotides
EP4382621A3 (en) 2021-07-27 2024-08-21 Gen-Probe Incorporated Compositions and methods for detecting c. coli and at least one of salmonella, c. jejuni, shigella, and stec
EP4424242A1 (en) 2021-10-29 2024-09-04 Seegene, Inc. Sample collecting swab tool and method for detection of respiratory pathogen
EP4282980A1 (en) 2022-05-23 2023-11-29 Mobidiag Oy Methods for amplifying a nucleic acid
WO2024015331A1 (en) 2022-07-12 2024-01-18 Genentech, Inc. Therapeutic and diagnostic methods for multiple sclerosis
KR102559124B1 (ko) 2022-08-25 2023-07-26 주식회사 엔젠바이오 Flt3 유전자 증폭용 조성물 및 이의 용도
WO2024054924A1 (en) 2022-09-08 2024-03-14 Gen-Probe Incorporated Method of detecting nucleic acid analytes using dual-specificity primers
WO2024081776A1 (en) 2022-10-13 2024-04-18 Gen-Probe Incorporated Cellularity control for nucleic acid assays
WO2024137379A2 (en) 2022-12-19 2024-06-27 Gen-Probe Incorporated Compositions and methods for detecting gastrointestinal pathogens
EP4389887A1 (en) 2022-12-21 2024-06-26 Mobidiag Oy Isolating and lysing cells
EP4389911A1 (en) 2022-12-21 2024-06-26 Mobidiag Oy Methods and systems for isolating an analyte
WO2024161179A1 (en) 2023-01-31 2024-08-08 Mobidiag Oy Compositions and methods for detecting stx nucleic acids
WO2024192338A1 (en) 2023-03-16 2024-09-19 Gen-Probe Incorporated Compositions and methods for detecting gastrointestinal parasites
EP4454758A1 (en) 2023-04-28 2024-10-30 Mobidiag Oy Nucleic acid amplification process controls
EP4455303A1 (en) 2023-04-28 2024-10-30 Mobidiag Oy Nucleic acid amplification process controls
EP4455304A1 (en) 2023-04-28 2024-10-30 Mobidiag Oy Nucleic acid amplification process controls
WO2024233375A1 (en) 2023-05-05 2024-11-14 Gen-Probe Incorporated Method and system for improving specificity of analyte detection using real-time nucleic acid amplification
FI20235652A1 (en) 2023-06-12 2024-12-13 Mobidiag Oy Methods for isolating microbial analyte

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683194A (en) 1984-05-29 1987-07-28 Cetus Corporation Method for detection of polymorphic restriction sites and nucleic acid sequences
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
AU606043B2 (en) 1985-03-28 1991-01-31 F. Hoffmann-La Roche Ag Detection of viruses by amplification and hybridization
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
DK171161B1 (da) * 1985-03-28 1996-07-08 Hoffmann La Roche Fremgangsmåde til påvisning af forekomst eller fravær af mindst én specifik nukleinsyresekvens i en prøve eller til skelnen mellem to forskellige nukleinsyresekvenser i denne prøve
EP0224126A3 (en) * 1985-11-25 1989-02-01 The University of Calgary Covalently linked complementary oligodeoxynucleotides as universal nucleic acid sequencing primer linkers
WO1987003451A1 (en) * 1985-12-05 1987-06-18 Fred Hutchinson Cancer Research Center Anti-sense rna for treatment of retroviral disease states
EP0272098A3 (en) * 1986-12-15 1990-06-06 City Of Hope National Medical Center Method for amplification and detection of rna sequences
IL86724A (en) * 1987-06-19 1995-01-24 Siska Diagnostics Inc Methods and kits for amplification and testing of nucleic acid sequences
CA1340843C (en) * 1987-07-31 1999-12-07 J. Lawrence Burg Selective amplification of target polynucleotide sequences
WO1989010315A1 (en) 1988-04-30 1989-11-02 Korfmacher Trading Gmbh Storage or supporting mat, in particular for packaging purposes
US5130238A (en) * 1988-06-24 1992-07-14 Cangene Corporation Enhanced nucleic acid amplification process
JP2905144B2 (ja) 1995-09-14 1999-06-14 有限会社野々川商事 棒状化粧料容器
DE19636774C2 (de) 1996-09-10 1999-04-22 Herberts Gmbh Verfahren zur Anpassung einer Farbrezeptur

Also Published As

Publication number Publication date
DE3855064T2 (de) 1996-08-29
EP0682120A1 (en) 1995-11-15
US6410276B1 (en) 2002-06-25
PT88168B (pt) 1995-03-01
FI891436A0 (fi) 1989-03-23
JPH02501532A (ja) 1990-05-31
WO1989001050A1 (en) 1989-02-09
DK153289A (da) 1989-03-30
FI891436L (fi) 1989-03-23
ES2086298T3 (es) 1996-07-01
EP0682120B1 (en) 2001-02-14
CA1340843C (en) 1999-12-07
DK153289D0 (da) 1989-03-30
EP0310229A1 (en) 1989-04-05
DE3856455T2 (de) 2001-07-26
KR960013577B1 (ko) 1996-10-09
JP2774121B2 (ja) 1998-07-09
PT88168A (pt) 1989-06-30
US5437990A (en) 1995-08-01
DE3855064D1 (de) 1996-04-11
IE882348L (en) 1989-01-31
EP0310229B1 (en) 1996-03-06
ATE135053T1 (de) 1996-03-15
IE72468B1 (en) 1997-04-09
DE3856455D1 (de) 2001-03-22

Similar Documents

Publication Publication Date Title
KR890701764A (ko) 표적 뉴클레오티드 서열의 선택적 증식
US5744308A (en) Chimera oligonucleotide and its utilization for obtaining transcripts of a nucleic acid
US6090591A (en) Selective amplification of target polynucleotide sequences
US5627054A (en) Competitor primer asymmetric polymerase chain reaction
JP3080178B2 (ja) 核酸配列の増幅方法およびそのための試薬キット
JP2020182453A5 (ko)
JP3644976B2 (ja) 核酸の増幅の改良法
JP3737734B2 (ja) 修飾プライマーを使用する増幅
CA2980624C (en) Solid phase nucleic acid target capture and replication using strand displacing polymerases
KR900018376A (ko) 리보핵산(rna)의 합성방법
JP2021100429A (ja) 標的核酸の検出方法
KR890013184A (ko) 핵산을 증폭시키는 방법
WO2000070095A2 (en) Homogeneous isothermal amplification and detection of nucleic acids using a template switch oligonucleotide
NO933136D0 (no) Bestemmelse av nukleinsyrer ved polymerasekstensjon av oligonukleotider ved anvendelse av terminatorblandinger
CA2639819A1 (en) Selective terminal tagging of nucleic acids
JP2002534986A5 (ko)
CN101680033A (zh) 基因表达的测定方法
US20090023151A1 (en) Method For The Labeling And Detection Of Small Polynucleotides
RU2007118546A (ru) Реагент и способ предотвращения по меньшей мере одного ошибочного старта в амплификации полимеразной цепной реакции, наборы реагентов и олигонуклеотидов для осуществления амплификации посредством полимеразной цепной реакции
KR920006510A (ko) 사람 유두종 바이러스의 증폭 및 검출을 위한 타입-특이 탐침, pcr프라이머 및 lcr탐침으로서 유용한 뉴클레오타이드 서열, 및 관련된 키트 및 방법
Morse Direct selection of RNA beacon aptamers
CN1271019A (zh) 通过载体核酸增强核酸扩增的特异性
JP2013524841A (ja) 一本鎖オーバーハングを有する二本鎖核酸を生成するための方法
White et al. Concatemer chain reaction: a Taq DNA polymerase-mediated mechanism for generating long tandemly repetitive DNA sequences
EP3252168B1 (en) Pcr primer linked to complementary nucleotide sequence or complementary nucleotide sequence including mis-matched nucleotides and method for amplifying nucleic acid using same

Legal Events

Date Code Title Description
PA0109 Patent application

Patent event code: PA01091R01D

Comment text: Patent Application

Patent event date: 19890330

PG1501 Laying open of application
A201 Request for examination
PA0201 Request for examination

Patent event code: PA02012R01D

Patent event date: 19930715

Comment text: Request for Examination of Application

Patent event code: PA02011R01I

Patent event date: 19890330

Comment text: Patent Application

E902 Notification of reason for refusal
PE0902 Notice of grounds for rejection

Comment text: Notification of reason for refusal

Patent event date: 19960118

Patent event code: PE09021S01D

G160 Decision to publish patent application
PG1605 Publication of application before grant of patent

Comment text: Decision on Publication of Application

Patent event code: PG16051S01I

Patent event date: 19960913

E701 Decision to grant or registration of patent right
PE0701 Decision of registration

Patent event code: PE07011S01D

Comment text: Decision to Grant Registration

Patent event date: 19970106

GRNT Written decision to grant
PR0701 Registration of establishment

Comment text: Registration of Establishment

Patent event date: 19970326

Patent event code: PR07011E01D

PR1002 Payment of registration fee

Payment date: 19970326

End annual number: 3

Start annual number: 1

PR1001 Payment of annual fee

Payment date: 19991008

Start annual number: 4

End annual number: 4

PR1001 Payment of annual fee

Payment date: 20000923

Start annual number: 5

End annual number: 5

PR1001 Payment of annual fee

Payment date: 20011004

Start annual number: 6

End annual number: 6

PR1001 Payment of annual fee

Payment date: 20020917

Start annual number: 7

End annual number: 7

PR1001 Payment of annual fee

Payment date: 20030923

Start annual number: 8

End annual number: 8

PR1001 Payment of annual fee

Payment date: 20041004

Start annual number: 9

End annual number: 9

PR1001 Payment of annual fee

Payment date: 20051005

Start annual number: 10

End annual number: 10

PR1001 Payment of annual fee

Payment date: 20060929

Start annual number: 11

End annual number: 11

PR1001 Payment of annual fee

Payment date: 20070907

Start annual number: 12

End annual number: 12

PR1001 Payment of annual fee

Payment date: 20080909

Start annual number: 13

End annual number: 13

PR1001 Payment of annual fee

Payment date: 20090916

Start annual number: 14

End annual number: 14

FPAY Annual fee payment

Payment date: 20100916

Year of fee payment: 15

PR1001 Payment of annual fee

Payment date: 20100916

Start annual number: 15

End annual number: 15

EXPY Expiration of term
PC1801 Expiration of term