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IL121441A - Thermostable DNA polymerase enzymes their preparation and their use - Google Patents

Thermostable DNA polymerase enzymes their preparation and their use

Info

Publication number
IL121441A
IL121441A IL12144197A IL12144197A IL121441A IL 121441 A IL121441 A IL 121441A IL 12144197 A IL12144197 A IL 12144197A IL 12144197 A IL12144197 A IL 12144197A IL 121441 A IL121441 A IL 121441A
Authority
IL
Israel
Prior art keywords
residue
sequence
dna
polymerases
thermostable dna
Prior art date
Application number
IL12144197A
Other languages
English (en)
Other versions
IL121441A0 (en
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Publication of IL121441A0 publication Critical patent/IL121441A0/xx
Publication of IL121441A publication Critical patent/IL121441A/xx

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
IL12144197A 1996-08-06 1997-07-31 Thermostable DNA polymerase enzymes their preparation and their use IL121441A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US2337696P 1996-08-06 1996-08-06

Publications (2)

Publication Number Publication Date
IL121441A0 IL121441A0 (en) 1998-01-04
IL121441A true IL121441A (en) 2000-07-16

Family

ID=21814728

Family Applications (1)

Application Number Title Priority Date Filing Date
IL12144197A IL121441A (en) 1996-08-06 1997-07-31 Thermostable DNA polymerase enzymes their preparation and their use

Country Status (21)

Country Link
US (1) US5939292A (cs)
EP (1) EP0823479B1 (cs)
JP (1) JP3170229B2 (cs)
KR (1) KR100510619B1 (cs)
CN (1) CN1154733C (cs)
AT (1) ATE278020T1 (cs)
AU (1) AU714929B2 (cs)
BR (1) BR9704260B1 (cs)
CA (1) CA2210951C (cs)
CZ (1) CZ293215B6 (cs)
DE (1) DE69730926T2 (cs)
DK (1) DK0823479T3 (cs)
ES (1) ES2227640T3 (cs)
HU (1) HUP9701341A3 (cs)
IL (1) IL121441A (cs)
MX (1) MX9705961A (cs)
NO (1) NO322135B1 (cs)
PL (1) PL321478A1 (cs)
RU (1) RU2235773C2 (cs)
TW (1) TW528802B (cs)
UA (1) UA47423C2 (cs)

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US6395524B2 (en) * 1996-11-27 2002-05-28 University Of Washington Thermostable polymerases having altered fidelity and method of identifying and using same
US20030215857A1 (en) * 1996-12-20 2003-11-20 Roche Diagnostics Gmbh Method for the direct, exponential amplification and sequencing of DNA molecules and its application
CA2283789C (en) 1997-03-12 2005-07-05 The Perkin-Elmer Corporation Dna polymerases having improved labeled nucleotide incorporation properties
US6346379B1 (en) 1997-09-11 2002-02-12 F. Hoffman-La Roche Ag Thermostable DNA polymerases incorporating nucleoside triphosphates labeled with fluorescein family dyes
US6777188B2 (en) 1998-10-01 2004-08-17 Variagenics, Inc. Genotyping by mass spectrometric analysis of allelic fragments
US6994998B1 (en) 1998-10-01 2006-02-07 Sequenom, Inc. Base-modified nucleotides and their use for polymorphism detection
US6500650B1 (en) 1998-10-01 2002-12-31 Variagenics, Inc. Method for identifying polymorphisms
US6610492B1 (en) 1998-10-01 2003-08-26 Variagenics, Inc. Base-modified nucleotides and cleavage of polynucleotides incorporating them
US6440705B1 (en) 1998-10-01 2002-08-27 Vincent P. Stanton, Jr. Method for analyzing polynucleotides
US6566059B1 (en) 1998-10-01 2003-05-20 Variagenics, Inc. Method for analyzing polynucleotides
US6855500B2 (en) 1998-10-01 2005-02-15 Sequenom, Inc. Fluorescence-based genotyping
US6458945B1 (en) 1998-10-01 2002-10-01 Variagenics, Inc. Method for analyzing polynucleotides
WO2000068411A1 (en) * 1999-05-12 2000-11-16 Invitrogen Corporation Compositions and methods for enhanced sensitivity and specificity of nucleic acid synthesis
GB9921318D0 (en) * 1999-09-09 1999-11-10 Kristensen Tom Chimeric molecules
US6329178B1 (en) * 2000-01-14 2001-12-11 University Of Washington DNA polymerase mutant having one or more mutations in the active site
US7179590B2 (en) 2000-04-18 2007-02-20 Roche Molecular Systems, Inc High temperature reverse transcription using mutant DNA polymerases
US20030009295A1 (en) * 2001-03-14 2003-01-09 Victor Markowitz System and method for retrieving and using gene expression data from multiple sources
US6214557B1 (en) * 2000-06-06 2001-04-10 Washington University Cold sensitive mutant DNA polymerases
EP1373889A2 (en) * 2000-07-31 2004-01-02 Maxygen, Inc. Biosensors, reagents and diagnostic applications of directed evolution
JP4741170B2 (ja) * 2000-10-11 2011-08-03 アプライド バイオシステムズ リミテッド ライアビリティー カンパニー アニオン性リンカーを有する蛍光核酸塩基結合体
ZA200303132B (en) * 2000-10-20 2004-09-23 Expression Diagnostics Inc Leukocyte expression profiling.
US7235358B2 (en) 2001-06-08 2007-06-26 Expression Diagnostics, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
US6905827B2 (en) 2001-06-08 2005-06-14 Expression Diagnostics, Inc. Methods and compositions for diagnosing or monitoring auto immune and chronic inflammatory diseases
US7026121B1 (en) 2001-06-08 2006-04-11 Expression Diagnostics, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
US6887690B2 (en) * 2001-06-22 2005-05-03 Pe Corporation Dye-labeled ribonucleotide triphosphates
WO2003025132A2 (en) * 2001-09-14 2003-03-27 Invitrogen Corporation Dna polymerases and mutants thereof
US20050042629A1 (en) 2002-09-13 2005-02-24 Applera Corporation Thermus scotoductus nucleic acid polymerases
WO2003048308A2 (en) 2001-11-30 2003-06-12 Applera Corporation Thermus brockianus nucleic acid polymerases
US6723546B2 (en) * 2002-03-26 2004-04-20 New England Biolabs, Inc. Method for cloning and expression of BsaI restriction endonuclease and BsaI methylase in E. coli
US7148049B2 (en) 2002-04-02 2006-12-12 Roche Molecular Systems, Inc. Thermostable or thermoactive DNA polymerase molecules with attenuated 3′-5′ exonuclease activity
WO2004094986A2 (en) 2003-04-16 2004-11-04 Handylab, Inc. System and method for electrochemical detection of biological compounds
US7892745B2 (en) 2003-04-24 2011-02-22 Xdx, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
US7572581B2 (en) * 2003-06-30 2009-08-11 Roche Molecular Systems, Inc. 2′-terminator nucleotide-related methods and systems
US7947817B2 (en) * 2003-06-30 2011-05-24 Roche Molecular Systems, Inc. Synthesis and compositions of 2'-terminator nucleotides
EP1493824A1 (en) * 2003-07-02 2005-01-05 Consortium National de Recherche en Genomique (CNRG) Method for detection of mutations in DNA
JP4127204B2 (ja) * 2003-12-17 2008-07-30 セイコーエプソン株式会社 液晶表示装置の製造方法
US20080311627A1 (en) * 2004-06-17 2008-12-18 Epigenomics Ag Compositions and Methods for Preventing Carry-Over Contamination in Nucleic Acid Amplification Reactions
US7745125B2 (en) 2004-06-28 2010-06-29 Roche Molecular Systems, Inc. 2′-terminator related pyrophosphorolysis activated polymerization
US7645575B2 (en) 2004-09-08 2010-01-12 Xdx, Inc. Genes useful for diagnosing and monitoring inflammation related disorders
US7890268B2 (en) * 2004-12-28 2011-02-15 Roche Molecular Systems, Inc. De-novo sequencing of nucleic acids
US20060292578A1 (en) * 2005-06-28 2006-12-28 Weidong Zheng DNA polymerase blends and mutant DNA polymerases
EP1907584A2 (en) * 2005-06-28 2008-04-09 Agencourt Personal Genomics Corporation Methods of producing and sequencing modified polynucleotides
EP1762629B1 (en) 2005-09-12 2009-11-11 Roche Diagnostics GmbH Detection of biological DNA
US20070154894A1 (en) * 2005-12-30 2007-07-05 Affymetrix, Inc. Labeling and non-enzymatic fragmentation of cDNA using a ribonucleoside triphosphate analog
US7993832B2 (en) 2006-08-14 2011-08-09 Xdx, Inc. Methods and compositions for diagnosing and monitoring the status of transplant rejection and immune disorders
EP2102367A2 (en) 2006-11-09 2009-09-23 XDX, Inc. Methods for diagnosing and monitoring the status of systemic lupus erythematosus
US20080242560A1 (en) * 2006-11-21 2008-10-02 Gunderson Kevin L Methods for generating amplified nucleic acid arrays
US10150990B2 (en) 2008-04-21 2018-12-11 Roche Molecular Systems, Inc. Ribonucleotide tag nucleic acid detection
US8951940B2 (en) 2010-04-01 2015-02-10 Illumina, Inc. Solid-phase clonal amplification and related methods
SG190377A1 (en) 2010-11-24 2013-06-28 Kaneka Corp Amplified nucleic acid detection method and detection device
WO2012146260A1 (de) 2011-04-23 2012-11-01 Biolytix Ag Herstellung und verwendung von proteinen in der molekularbiologie
US9783844B2 (en) * 2012-04-27 2017-10-10 Kaneka Corporation Method for amplifying nucleic acid and method for detecting amplified nucleic acid
TWI487789B (zh) 2012-12-24 2015-06-11 Ind Tech Res Inst 單離的脫氧核糖核酸聚合酶、套組與其應用
ES2713653T3 (es) 2013-03-15 2019-05-23 Ibis Biosciences Inc Análogos nucleotídicos para secuenciación
ES2805004T3 (es) 2013-08-19 2021-02-10 Abbott Molecular Inc Análogos de nucleótidos
US10392652B2 (en) 2013-11-22 2019-08-27 Kaneka Corporation Micro RNA detection method using two primers to produce an amplified double stranded DNA fragment having a single stranded region at one end
CN103966182A (zh) * 2014-04-30 2014-08-06 厦门安普利生物工程有限公司 一种提取纯化Tth DNA聚合酶的方法
KR102154812B1 (ko) * 2014-09-29 2020-09-11 박진우 광고 및 태양광 충전 기능을 가진 가방
EP3091026B1 (en) 2015-05-08 2019-02-20 Centrillion Technology Holdings Corporation Disulfide-linked reversible terminators
WO2019014359A2 (en) * 2017-07-12 2019-01-17 The Scripps Research Institute POLYMERASE CHAIN TRANSCRIPTION (PCT): EXPONENTIAL SYNTHESIS OF RNA AND MODIFIED RNA
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CN108130318B (zh) * 2018-02-28 2020-07-14 深圳市艾伟迪生物科技有限公司 突变型Taq DNA聚合酶、免核酸提取直接PCR扩增的试剂盒及其应用
CN113195511A (zh) 2018-09-28 2021-07-30 生捷科技控股公司 二硫键连接的可逆终止剂
US11060075B2 (en) 2018-10-29 2021-07-13 Codexis, Inc. Engineered DNA polymerase variants
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US4889818A (en) * 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
US5912155A (en) * 1994-09-30 1999-06-15 Life Technologies, Inc. Cloned DNA polymerases from Thermotoga neapolitana
US5614365A (en) * 1994-10-17 1997-03-25 President & Fellow Of Harvard College DNA polymerase having modified nucleotide binding site for DNA sequencing
DE655506T1 (de) * 1994-10-17 1995-09-28 President And Fellows Of Harvard College, Cambridge, Mass. DNS Polymerase mit Veränderten Nukleotiden Bindungstelle.

Also Published As

Publication number Publication date
NO973595D0 (no) 1997-08-05
BR9704260B1 (pt) 2010-09-08
EP0823479A2 (en) 1998-02-11
UA47423C2 (uk) 2002-07-15
PL321478A1 (en) 1998-02-16
RU2235773C2 (ru) 2004-09-10
HU9701341D0 (en) 1997-09-29
CA2210951C (en) 2009-02-10
NO322135B1 (no) 2006-08-21
DK0823479T3 (da) 2005-01-31
US5939292A (en) 1999-08-17
KR19980018395A (ko) 1998-06-05
BR9704260A (pt) 1998-09-15
CZ293215B6 (cs) 2004-03-17
TW528802B (en) 2003-04-21
NO973595L (no) 1998-02-09
MX9705961A (es) 1998-02-28
CA2210951A1 (en) 1998-02-06
CN1154733C (zh) 2004-06-23
EP0823479B1 (en) 2004-09-29
AU3319797A (en) 1998-02-12
KR100510619B1 (ko) 2005-12-21
IL121441A0 (en) 1998-01-04
HUP9701341A3 (en) 2000-12-28
AU714929B2 (en) 2000-01-13
ATE278020T1 (de) 2004-10-15
JPH1066588A (ja) 1998-03-10
HUP9701341A2 (hu) 1999-06-28
EP0823479A3 (en) 2000-03-29
JP3170229B2 (ja) 2001-05-28
DE69730926T2 (de) 2006-02-23
CN1177640A (zh) 1998-04-01
DE69730926D1 (de) 2004-11-04
ES2227640T3 (es) 2005-04-01
CZ229997A3 (cs) 1998-06-17

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