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HUP9701341A3 - Modified thermostable dns polymerase, process for producing thereof, nucleic acid codind the enzyme, vector containing the nucleic acid, a composition containing thereof - Google Patents

Modified thermostable dns polymerase, process for producing thereof, nucleic acid codind the enzyme, vector containing the nucleic acid, a composition containing thereof

Info

Publication number
HUP9701341A3
HUP9701341A3 HU9701341A HUP9701341A HUP9701341A3 HU P9701341 A3 HUP9701341 A3 HU P9701341A3 HU 9701341 A HU9701341 A HU 9701341A HU P9701341 A HUP9701341 A HU P9701341A HU P9701341 A3 HUP9701341 A3 HU P9701341A3
Authority
HU
Hungary
Prior art keywords
nucleic acid
codind
enzyme
producing
composition containing
Prior art date
Application number
HU9701341A
Other languages
English (en)
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Publication of HU9701341D0 publication Critical patent/HU9701341D0/hu
Publication of HUP9701341A2 publication Critical patent/HUP9701341A2/hu
Publication of HUP9701341A3 publication Critical patent/HUP9701341A3/hu

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
HU9701341A 1996-08-06 1997-08-01 Modified thermostable dns polymerase, process for producing thereof, nucleic acid codind the enzyme, vector containing the nucleic acid, a composition containing thereof HUP9701341A3 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US2337696P 1996-08-06 1996-08-06

Publications (3)

Publication Number Publication Date
HU9701341D0 HU9701341D0 (en) 1997-09-29
HUP9701341A2 HUP9701341A2 (hu) 1999-06-28
HUP9701341A3 true HUP9701341A3 (en) 2000-12-28

Family

ID=21814728

Family Applications (1)

Application Number Title Priority Date Filing Date
HU9701341A HUP9701341A3 (en) 1996-08-06 1997-08-01 Modified thermostable dns polymerase, process for producing thereof, nucleic acid codind the enzyme, vector containing the nucleic acid, a composition containing thereof

Country Status (21)

Country Link
US (1) US5939292A (hu)
EP (1) EP0823479B1 (hu)
JP (1) JP3170229B2 (hu)
KR (1) KR100510619B1 (hu)
CN (1) CN1154733C (hu)
AT (1) ATE278020T1 (hu)
AU (1) AU714929B2 (hu)
BR (1) BR9704260B1 (hu)
CA (1) CA2210951C (hu)
CZ (1) CZ293215B6 (hu)
DE (1) DE69730926T2 (hu)
DK (1) DK0823479T3 (hu)
ES (1) ES2227640T3 (hu)
HU (1) HUP9701341A3 (hu)
IL (1) IL121441A (hu)
MX (1) MX9705961A (hu)
NO (1) NO322135B1 (hu)
PL (1) PL321478A1 (hu)
RU (1) RU2235773C2 (hu)
TW (1) TW528802B (hu)
UA (1) UA47423C2 (hu)

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US20030215857A1 (en) * 1996-12-20 2003-11-20 Roche Diagnostics Gmbh Method for the direct, exponential amplification and sequencing of DNA molecules and its application
EP2202312B1 (en) * 1997-03-12 2016-01-06 Applied Biosystems, LLC DNA polymerases having improved labelled nucleotide incorporation properties
CA2243985C (en) * 1997-09-11 2004-08-10 F. Hoffmann-La Roche Ag Thermostable dna polymerases incorporating nucleoside triphosphates labeled with fluorescein family dyes
US6855500B2 (en) 1998-10-01 2005-02-15 Sequenom, Inc. Fluorescence-based genotyping
US6458945B1 (en) 1998-10-01 2002-10-01 Variagenics, Inc. Method for analyzing polynucleotides
US6610492B1 (en) 1998-10-01 2003-08-26 Variagenics, Inc. Base-modified nucleotides and cleavage of polynucleotides incorporating them
US6440705B1 (en) 1998-10-01 2002-08-27 Vincent P. Stanton, Jr. Method for analyzing polynucleotides
US6777188B2 (en) 1998-10-01 2004-08-17 Variagenics, Inc. Genotyping by mass spectrometric analysis of allelic fragments
US6994998B1 (en) 1998-10-01 2006-02-07 Sequenom, Inc. Base-modified nucleotides and their use for polymorphism detection
US6566059B1 (en) 1998-10-01 2003-05-20 Variagenics, Inc. Method for analyzing polynucleotides
US6500650B1 (en) 1998-10-01 2002-12-31 Variagenics, Inc. Method for identifying polymorphisms
WO2000068411A1 (en) * 1999-05-12 2000-11-16 Invitrogen Corporation Compositions and methods for enhanced sensitivity and specificity of nucleic acid synthesis
GB9921318D0 (en) * 1999-09-09 1999-11-10 Kristensen Tom Chimeric molecules
US6329178B1 (en) 2000-01-14 2001-12-11 University Of Washington DNA polymerase mutant having one or more mutations in the active site
US7179590B2 (en) * 2000-04-18 2007-02-20 Roche Molecular Systems, Inc High temperature reverse transcription using mutant DNA polymerases
US20030009295A1 (en) * 2001-03-14 2003-01-09 Victor Markowitz System and method for retrieving and using gene expression data from multiple sources
US6214557B1 (en) * 2000-06-06 2001-04-10 Washington University Cold sensitive mutant DNA polymerases
US20020127623A1 (en) * 2000-07-31 2002-09-12 Maxygen, Inc. Biosensors, reagents and diagnostic applications of directed evolution
ATE431355T1 (de) * 2000-10-11 2009-05-15 Applera Corp Fluoreszierende nukleobasekonjugate mit anionische linker
NZ525336A (en) * 2000-10-20 2006-03-31 Expression Diagnostics Inc Leukocyte expression profiling
US7026121B1 (en) 2001-06-08 2006-04-11 Expression Diagnostics, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
US6905827B2 (en) 2001-06-08 2005-06-14 Expression Diagnostics, Inc. Methods and compositions for diagnosing or monitoring auto immune and chronic inflammatory diseases
US7235358B2 (en) 2001-06-08 2007-06-26 Expression Diagnostics, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
US6887690B2 (en) * 2001-06-22 2005-05-03 Pe Corporation Dye-labeled ribonucleotide triphosphates
WO2003025132A2 (en) * 2001-09-14 2003-03-27 Invitrogen Corporation Dna polymerases and mutants thereof
US20050042629A1 (en) 2002-09-13 2005-02-24 Applera Corporation Thermus scotoductus nucleic acid polymerases
US7052877B2 (en) 2001-11-30 2006-05-30 Applera Corporation Thermus brockianus nucleic acid polymerases
US6723546B2 (en) * 2002-03-26 2004-04-20 New England Biolabs, Inc. Method for cloning and expression of BsaI restriction endonuclease and BsaI methylase in E. coli
US7148049B2 (en) 2002-04-02 2006-12-12 Roche Molecular Systems, Inc. Thermostable or thermoactive DNA polymerase molecules with attenuated 3′-5′ exonuclease activity
US7820030B2 (en) 2003-04-16 2010-10-26 Handylab, Inc. System and method for electrochemical detection of biological compounds
US7892745B2 (en) 2003-04-24 2011-02-22 Xdx, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
US7947817B2 (en) * 2003-06-30 2011-05-24 Roche Molecular Systems, Inc. Synthesis and compositions of 2'-terminator nucleotides
US7572581B2 (en) * 2003-06-30 2009-08-11 Roche Molecular Systems, Inc. 2′-terminator nucleotide-related methods and systems
EP1493824A1 (en) * 2003-07-02 2005-01-05 Consortium National de Recherche en Genomique (CNRG) Method for detection of mutations in DNA
JP4127204B2 (ja) * 2003-12-17 2008-07-30 セイコーエプソン株式会社 液晶表示装置の製造方法
ATE456672T1 (de) * 2004-06-17 2010-02-15 Epigenomics Ag Zusammensetzungen und verfahren zur verhinderung des mitschleppens einer kontamination in nukleinsäure-amplifikationsreaktionen
US7745125B2 (en) 2004-06-28 2010-06-29 Roche Molecular Systems, Inc. 2′-terminator related pyrophosphorolysis activated polymerization
WO2006029184A2 (en) 2004-09-08 2006-03-16 Expression Diagnostics, Inc. Genes useful for diagnosing and monitoring inflammation related disorders
US7890268B2 (en) * 2004-12-28 2011-02-15 Roche Molecular Systems, Inc. De-novo sequencing of nucleic acids
US20060292578A1 (en) * 2005-06-28 2006-12-28 Weidong Zheng DNA polymerase blends and mutant DNA polymerases
JP2008546424A (ja) * 2005-06-28 2008-12-25 アジェンコート パーソナル ジェノミクス コーポレイション 修飾ポリヌクレオチドを作製する方法および配列決定する方法
EP1762629B1 (en) 2005-09-12 2009-11-11 Roche Diagnostics GmbH Detection of biological DNA
US20070154894A1 (en) * 2005-12-30 2007-07-05 Affymetrix, Inc. Labeling and non-enzymatic fragmentation of cDNA using a ribonucleoside triphosphate analog
WO2008021431A2 (en) 2006-08-14 2008-02-21 Xdx, Inc. Methods and compositions for diagnosing and monitoring the status of transplant rejection and immune disorders
US8148067B2 (en) 2006-11-09 2012-04-03 Xdx, Inc. Methods for diagnosing and monitoring the status of systemic lupus erythematosus
US20080242560A1 (en) * 2006-11-21 2008-10-02 Gunderson Kevin L Methods for generating amplified nucleic acid arrays
US10150990B2 (en) 2008-04-21 2018-12-11 Roche Molecular Systems, Inc. Ribonucleotide tag nucleic acid detection
US8951940B2 (en) 2010-04-01 2015-02-10 Illumina, Inc. Solid-phase clonal amplification and related methods
WO2012070618A1 (ja) 2010-11-24 2012-05-31 株式会社カネカ 増幅核酸検出方法及び検出デバイス
WO2012146260A1 (de) 2011-04-23 2012-11-01 Biolytix Ag Herstellung und verwendung von proteinen in der molekularbiologie
EP2843058B1 (en) * 2012-04-27 2019-12-18 Kaneka Corporation Method for amplifying nucleic acid and method for detecting amplified nucleic acid
TWI487789B (zh) 2012-12-24 2015-06-11 Ind Tech Res Inst 單離的脫氧核糖核酸聚合酶、套組與其應用
EP2970366B1 (en) 2013-03-15 2019-01-16 Ibis Biosciences, Inc. Nucleotide analogs for sequencing
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WO2015076356A1 (ja) 2013-11-22 2015-05-28 株式会社カネカ 短鎖ｒｎａの検出方法
CN103966182A (zh) * 2014-04-30 2014-08-06 厦门安普利生物工程有限公司 一种提取纯化Tth DNA聚合酶的方法
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US11640848B2 (en) 2017-09-20 2023-05-02 Regeneron Pharmaceuticals, Inc. Immunotherapy methods for patients whose tumors carry a high passenger gene mutation burden
CN108130318B (zh) * 2018-02-28 2020-07-14 深圳市艾伟迪生物科技有限公司 突变型Taq DNA聚合酶、免核酸提取直接PCR扩增的试剂盒及其应用
CN113195511A (zh) 2018-09-28 2021-07-30 生捷科技控股公司 二硫键连接的可逆终止剂
CN113316635A (zh) 2018-10-29 2021-08-27 科德克希思公司 工程化dna聚合酶变体
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US5614365A (en) * 1994-10-17 1997-03-25 President & Fellow Of Harvard College DNA polymerase having modified nucleotide binding site for DNA sequencing

Also Published As

Publication number Publication date
US5939292A (en) 1999-08-17
CA2210951C (en) 2009-02-10
TW528802B (en) 2003-04-21
BR9704260B1 (pt) 2010-09-08
EP0823479A3 (en) 2000-03-29
JP3170229B2 (ja) 2001-05-28
HU9701341D0 (en) 1997-09-29
CZ229997A3 (cs) 1998-06-17
KR100510619B1 (ko) 2005-12-21
PL321478A1 (en) 1998-02-16
ATE278020T1 (de) 2004-10-15
AU714929B2 (en) 2000-01-13
CN1154733C (zh) 2004-06-23
EP0823479B1 (en) 2004-09-29
RU2235773C2 (ru) 2004-09-10
CA2210951A1 (en) 1998-02-06
DE69730926T2 (de) 2006-02-23
IL121441A (en) 2000-07-16
IL121441A0 (en) 1998-01-04
CZ293215B6 (cs) 2004-03-17
DK0823479T3 (da) 2005-01-31
AU3319797A (en) 1998-02-12
MX9705961A (es) 1998-02-28
ES2227640T3 (es) 2005-04-01
KR19980018395A (ko) 1998-06-05
BR9704260A (pt) 1998-09-15
NO973595D0 (no) 1997-08-05
DE69730926D1 (de) 2004-11-04
HUP9701341A2 (hu) 1999-06-28
NO322135B1 (no) 2006-08-21
EP0823479A2 (en) 1998-02-11
JPH1066588A (ja) 1998-03-10
CN1177640A (zh) 1998-04-01
UA47423C2 (uk) 2002-07-15
NO973595L (no) 1998-02-09

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