FI121075B - Geneettisesti muokattu sienisolu, menetelmä rekombinanttituotteiden tuottamiseksi sekä DNA-molekyyli ja DNA-fragmentti ja näiden käyttö - Google Patents

Geneettisesti muokattu sienisolu, menetelmä rekombinanttituotteiden tuottamiseksi sekä DNA-molekyyli ja DNA-fragmentti ja näiden käyttö Download PDF

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Publication number: FI121075B
Authority: FI; Finland
Prior art keywords: fungal cell; dna; leu; ser; sequence
Prior art date: 1992-08-14

Application number

FI950628A

Other languages

English (en)

Finnish (fi)

Swedish (sv)

Other versions

FI950628L (fi

FI950628A0 (fi

Inventor

Reinhard Fleer

Alain Fournier

Widmar Tanner

Sabine Strahl-Bolsinger

Original Assignee

Novozymes Biopharma Dk As

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1992-08-14

Filing date

1995-02-13

Publication date

2010-06-30

1995-02-13 Application filed by Novozymes Biopharma Dk As filed Critical Novozymes Biopharma Dk As

1995-02-13 Publication of FI950628L publication Critical patent/FI950628L/fi

1995-02-13 Publication of FI950628A0 publication Critical patent/FI950628A0/fi

2010-06-30 Application granted granted Critical

2010-06-30 Publication of FI121075B publication Critical patent/FI121075B/fi

Links

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Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
- C12N9/1051—Hexosyltransferases (2.4.1)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts

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Life Sciences & Earth Sciences (AREA)
Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Engineering & Computer Science (AREA)
Chemical & Material Sciences (AREA)
Bioinformatics & Cheminformatics (AREA)
Organic Chemistry (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Biomedical Technology (AREA)
General Engineering & Computer Science (AREA)
Biotechnology (AREA)
Mycology (AREA)
Microbiology (AREA)
Biochemistry (AREA)
General Health & Medical Sciences (AREA)
Molecular Biology (AREA)
Plant Pathology (AREA)
Biophysics (AREA)
Physics & Mathematics (AREA)
Medicinal Chemistry (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

FI950628A 1992-08-14 1995-02-13 Geneettisesti muokattu sienisolu, menetelmä rekombinanttituotteiden tuottamiseksi sekä DNA-molekyyli ja DNA-fragmentti ja näiden käyttö FI121075B (fi)

Applications Claiming Priority (4)

Application Number	Priority Date	Filing Date	Title
DE4226971		1992-08-14
DE4226971A DE4226971C2 (de)	1992-08-14	1992-08-14	Modifizierte Pilzzellen und Verfahren zur Herstellung rekombinanter Produkte
PCT/EP1993/002179 WO1994004687A1 (en)	1992-08-14	1993-08-16	Modified fungal cells and method for producing recombinant products
EP9302179		1993-08-16

Publications (3)

Publication Number	Publication Date
FI950628L FI950628L (fi)	1995-02-13
FI950628A0 FI950628A0 (fi)	1995-02-13
FI121075B true FI121075B (fi)	2010-06-30

Family

ID=6465579

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
FI950628A FI121075B (fi)	1992-08-14	1995-02-13	Geneettisesti muokattu sienisolu, menetelmä rekombinanttituotteiden tuottamiseksi sekä DNA-molekyyli ja DNA-fragmentti ja näiden käyttö

Country Status (13)

Country	Link
US (1)	US5714377A (no)
EP (1)	EP0658203B1 (no)
JP (1)	JP3630424B2 (no)
AT (1)	ATE245701T1 (no)
AU (1)	AU679935B2 (no)
CA (1)	CA2140894C (no)
DE (3)	DE4226971C2 (no)
ES (1)	ES2203619T3 (no)
FI (1)	FI121075B (no)
NO (1)	NO320349B1 (no)
NZ (1)	NZ255350A (no)
WO (1)	WO1994004687A1 (no)
ZA (1)	ZA935915B (no)

Families Citing this family (57)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
FR2686899B1 (fr) *	1992-01-31	1995-09-01	Rhone Poulenc Rorer Sa	Nouveaux polypeptides biologiquement actifs, leur preparation et compositions pharmaceutiques les contenant.
US5728553A (en)	1992-09-23	1998-03-17	Delta Biotechnology Limited	High purity albumin and method of producing
JPH08173170A (ja) *	1994-05-25	1996-07-09	Kirin Brewery Co Ltd	キャンディダ・ユティリス酵母の形質転換系、およびそれによる異種遺伝子の発現
CA2221271A1 (en) *	1995-05-18	1996-11-21	Genencor International, Inc.	Expression of glycosyltransferase in aspergillus
GB9526733D0 (en) *	1995-12-30	1996-02-28	Delta Biotechnology Ltd	Fusion proteins
GB9902000D0 (en) *	1999-01-30	1999-03-17	Delta Biotechnology Ltd	Process
AU2007200859A1 (en) *	1999-01-30	2007-03-22	Novozymes Delta Limited	Process
CA2405709A1 (en) *	2000-04-12	2001-10-25	Human Genome Sciences, Inc.	Albumin fusion proteins
US6946134B1 (en)	2000-04-12	2005-09-20	Human Genome Sciences, Inc.	Albumin fusion proteins
US20050100991A1 (en) *	2001-04-12	2005-05-12	Human Genome Sciences, Inc.	Albumin fusion proteins
RU2003120093A (ru) *	2000-12-05	2005-01-27	Дзе Пенн Стейт Рисерч Фаундейшн (Us)	Способы и композиции для высокоэффективного получения гетерологичных белков в дрожжах
US7507413B2 (en)	2001-04-12	2009-03-24	Human Genome Sciences, Inc.	Albumin fusion proteins
US20050054051A1 (en) *	2001-04-12	2005-03-10	Human Genome Sciences, Inc.	Albumin fusion proteins
US20060084794A1 (en) *	2001-04-12	2006-04-20	Human Genome Sciences, Inc.	Albumin fusion proteins
US20050244931A1 (en) *	2001-04-12	2005-11-03	Human Genome Sciences, Inc.	Albumin fusion proteins
CA2383556A1 (en) *	2001-05-16	2002-11-16	Pharmacia & Upjohn Company	High-efficiency assay for protein mannosyl transferases
US7176278B2 (en)	2001-08-30	2007-02-13	Biorexis Technology, Inc.	Modified transferrin fusion proteins
AU2002332041A1 (en) *	2001-10-05	2003-04-22	Human Genome Sciences, Inc.	Albumin fusion proteins
ES2545090T3 (es) *	2001-12-21	2015-09-08	Human Genome Sciences, Inc.	Proteínas de fusión de albúmina y GCSF
WO2005003296A2 (en)	2003-01-22	2005-01-13	Human Genome Sciences, Inc.	Albumin fusion proteins
EP1463752A4 (en) *	2001-12-21	2005-07-13	Human Genome Sciences Inc	ALBUMIN FUSION PROTEINS
EP1471933A1 (en)	2002-02-07	2004-11-03	Delta Biotechnology Limited	Albumin-fused anti-angiogenesis peptides
NZ540743A (en) *	2002-12-20	2008-06-30	Unilever Plc	Preparation of antifreeze protein
GB0329722D0 (en)	2003-12-23	2004-01-28	Delta Biotechnology Ltd	Modified plasmid and use thereof
US9057061B2 (en)	2003-12-23	2015-06-16	Novozymes Biopharma Dk A/S	Gene expression technique
GB0329681D0 (en)	2003-12-23	2004-01-28	Delta Biotechnology Ltd	Gene expression technique
CA2628725A1 (en)	2005-11-15	2007-05-31	Glycofi, Inc.	Production of glycoproteins with reduced o-glycosylation
AU2012203729B2 (en) *	2005-11-22	2014-01-30	Glycofi, Inc	Production of glycoproteins with reduced O-glycosylation
EP1816201A1 (en) *	2006-02-06	2007-08-08	CSL Behring GmbH	Modified coagulation factor VIIa with extended half-life
WO2007132949A1 (ja)	2006-05-16	2007-11-22	National Institute Of Advanced Industrial Science And Technology	タンパク質の高分泌生産方法
JP5102833B2 (ja)	2006-07-24	2012-12-19	バイオレクシスファーマシューティカルコーポレーション	エキセンディン融合タンパク質
GB0711424D0 (en) *	2007-06-13	2007-07-25	Novozymes Delta Ltd	Recombinant transferrin mutants
CN101835801B (zh)	2007-08-08	2014-09-10	诺维信生物制药丹麦公司	转铁蛋白变体和偶联物
US8637435B2 (en) *	2007-11-16	2014-01-28	Merck Sharp & Dohme Corp.	Eukaryotic cell display systems
US20100311122A1 (en)	2008-02-20	2010-12-09	Glycofi, Inc	Vectors and yeast strains for protein production
CA2715212A1 (en)	2008-03-03	2009-09-11	Glycofi, Inc.	Surface display of recombinant proteins in lower eukaryotes
WO2010019487A1 (en) *	2008-08-12	2010-02-18	Glycofi, Inc.	Improved vectors and yeast strains for protein production: ca2+ atpase overexpression
US9120871B2 (en)	2009-01-23	2015-09-01	Novo Nordisk A/S	Process for preparing FGF21 with low degree of O-glycosylation
EP2617733A1 (en)	2009-02-06	2013-07-24	Novozymes Biopharma DK A/S	Purification process
KR101722961B1 (ko)	2009-02-11	2017-04-04	알부메딕스 에이/에스	알부민 변이체 및 접합체
US20120003695A1 (en)	2009-02-25	2012-01-05	Davidson Robert C	Metabolic engineering of a galactose assimilation pathway in the glycoengineered yeast pichia pastoris
US8748380B2 (en)	2009-10-30	2014-06-10	Novozymes Biopharma Dk A/S	Albumin variants
CN102725396A (zh)	2009-10-30	2012-10-10	默沙东公司	在缺乏二肽基氨肽酶活性的巴斯德毕赤酵母中生产治疗性蛋白的方法
MX2012009349A (es) *	2010-02-10	2013-02-11	Biocon Ltd	Metodo para reducir glucosilacion de proteinas, procedimientos y proteinas de los mismos.
WO2011106389A1 (en)	2010-02-24	2011-09-01	Merck Sharp & Dohme Corp.	Method for increasing n-glycosylation site occupancy on therapeutic glycoproteins produced in pichia pastoris
US10233228B2 (en)	2010-04-09	2019-03-19	Albumedix Ltd	Albumin derivatives and variants
CN103080130B (zh)	2010-05-27	2016-08-17	默沙东公司	制备具有改进特性的抗体的方法
JP5885191B2 (ja)	2011-01-04	2016-03-15	国立研究開発法人産業技術総合研究所	糖鎖改変酵母及びそれを用いた糖タンパク質の製造方法
US8936918B2 (en)	2011-02-25	2015-01-20	Merck Sharp & Dohme Corp.	Yeast strain for the production of proteins with modified O-glycosylation
EP2714732A4 (en)	2011-05-25	2014-12-10	Merck Sharp & Dohme	PROCESS FOR PREPARING FC-CONTAINING POLYPEPTIDES WITH IMPROVED PROPERTIES
EP2780364A2 (en)	2011-11-18	2014-09-24	Eleven Biotherapeutics, Inc.	Proteins with improved half-life and other properties
US9944691B2 (en)	2012-03-16	2018-04-17	Albumedix A/S	Albumin variants
GB2512156A (en)	2012-11-08	2014-09-24	Novozymes Biopharma Dk As	Albumin variants
EP3004156A1 (en)	2013-06-07	2016-04-13	Novo Nordisk A/S	Method for making mature insulin polypeptides
US20160376570A1 (en)	2013-07-04	2016-12-29	Novartis Ag	O-Mannosyltransferase Deficient Filamentous Fungal Cells and Methods of Use Thereof
CN108064266A (zh)	2014-07-21	2018-05-22	格利科斯芬兰公司	在丝状真菌中具有哺乳动物样n-聚糖的糖蛋白的制备
MX2018001825A (es)	2015-08-20	2018-05-07	Albumedix As	Variantes y conjugados de albumina.

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
DE2862495D1 (en) *	1977-11-08	1989-05-24	Genentech Inc	Plasmid for transforming bacterial host to render it capable of polypeptide expression
IT1203758B (it) *	1986-03-27	1989-02-23	Univ Roma	Vettori di clonazione e di espressione di geni eterologhi in lieviti e lieviti trasformati con tali vettori
US4929553A (en) *	1987-05-29	1990-05-29	Canadian Patents & Development Ltd.	Protease for specific processing of secreted proteins
FR2649991B2 (fr) *	1988-08-05	1994-03-04	Rhone Poulenc Sante	Utilisation de derives stables du plasmide pkd1 pour l'expression et la secretion de proteines heterologues dans les levures du genre kluyveromyces
FR2655660B1 (fr) *	1989-12-11	1992-03-20	Rhone Poulenc Sante	Nouveaux polypeptides, sequences d'adn permettant leur expression, procede de preparation et leur utilisation.

1992
- 1992-08-14 DE DE4226971A patent/DE4226971C2/de not_active Expired - Lifetime
- 1992-08-14 DE DE4244915A patent/DE4244915C2/de not_active Expired - Lifetime
1993
- 1993-08-13 ZA ZA935915A patent/ZA935915B/xx unknown
- 1993-08-16 WO PCT/EP1993/002179 patent/WO1994004687A1/en active IP Right Grant
- 1993-08-16 NZ NZ255350A patent/NZ255350A/en not_active IP Right Cessation
- 1993-08-16 JP JP50589294A patent/JP3630424B2/ja not_active Expired - Lifetime
- 1993-08-16 AT AT93919067T patent/ATE245701T1/de active
- 1993-08-16 EP EP93919067A patent/EP0658203B1/en not_active Expired - Lifetime
- 1993-08-16 AU AU49482/93A patent/AU679935B2/en not_active Expired
- 1993-08-16 ES ES93919067T patent/ES2203619T3/es not_active Expired - Lifetime
- 1993-08-16 CA CA002140894A patent/CA2140894C/en not_active Expired - Lifetime
- 1993-08-16 US US08/381,931 patent/US5714377A/en not_active Expired - Lifetime
- 1993-08-16 DE DE69333112T patent/DE69333112T2/de not_active Expired - Lifetime
1995
- 1995-02-13 NO NO19950534A patent/NO320349B1/no not_active IP Right Cessation
- 1995-02-13 FI FI950628A patent/FI121075B/fi not_active IP Right Cessation

Also Published As

Publication number	Publication date
NO320349B1 (no)	2005-11-28
ES2203619T3 (es)	2004-04-16
DE4226971A1 (de)	1994-02-17
NZ255350A (en)	1996-11-26
US5714377A (en)	1998-02-03
AU4948293A (en)	1994-03-15
NO950534L (no)	1995-04-05
FI950628L (fi)	1995-02-13
AU679935B2 (en)	1997-07-17
CA2140894C (en)	2010-03-02
EP0658203A1 (en)	1995-06-21
CA2140894A1 (en)	1994-03-03
DE69333112T2 (de)	2004-06-09
JP3630424B2 (ja)	2005-03-16
DE69333112D1 (de)	2003-08-28
NO950534D0 (no)	1995-02-13
FI950628A0 (fi)	1995-02-13
DE4226971C2 (de)	1997-01-16
JPH08500975A (ja)	1996-02-06
EP0658203B1 (en)	2003-07-23
ZA935915B (en)	1995-04-13
WO1994004687A1 (en)	1994-03-03
ATE245701T1 (de)	2003-08-15
DE4244915C2 (de)	1998-12-03

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