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DE69333112D1 - Modifizierte pilzzellen und methode zur herstelllung rekombinanter produkte - Google Patents

Modifizierte pilzzellen und methode zur herstelllung rekombinanter produkte

Info

Publication number
DE69333112D1
DE69333112D1 DE69333112T DE69333112T DE69333112D1 DE 69333112 D1 DE69333112 D1 DE 69333112D1 DE 69333112 T DE69333112 T DE 69333112T DE 69333112 T DE69333112 T DE 69333112T DE 69333112 D1 DE69333112 D1 DE 69333112D1
Authority
DE
Germany
Prior art keywords
pct
recombinant products
cells
production
sec
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69333112T
Other languages
English (en)
Other versions
DE69333112T2 (de
Inventor
Widmar Tanner
Sabine C O Ph Strahl-Bolsinger
Reinhard Fleer
Alain Fournier
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novozymes Biopharma DK AS
Original Assignee
Aventis Pharma SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Aventis Pharma SA filed Critical Aventis Pharma SA
Priority to DE69333112T priority Critical patent/DE69333112T2/de
Application granted granted Critical
Publication of DE69333112D1 publication Critical patent/DE69333112D1/de
Publication of DE69333112T2 publication Critical patent/DE69333112T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DE69333112T 1992-08-14 1993-08-16 Modifizierte pilzzellen und methode zur herstelllung rekombinanter produkte Expired - Lifetime DE69333112T2 (de)

Priority Applications (1)

Application Number Priority Date Filing Date Title
DE69333112T DE69333112T2 (de) 1992-08-14 1993-08-16 Modifizierte pilzzellen und methode zur herstelllung rekombinanter produkte

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DE4226971 1992-08-14
DE4226971A DE4226971C2 (de) 1992-08-14 1992-08-14 Modifizierte Pilzzellen und Verfahren zur Herstellung rekombinanter Produkte
DE69333112T DE69333112T2 (de) 1992-08-14 1993-08-16 Modifizierte pilzzellen und methode zur herstelllung rekombinanter produkte
PCT/EP1993/002179 WO1994004687A1 (en) 1992-08-14 1993-08-16 Modified fungal cells and method for producing recombinant products

Publications (2)

Publication Number Publication Date
DE69333112D1 true DE69333112D1 (de) 2003-08-28
DE69333112T2 DE69333112T2 (de) 2004-06-09

Family

ID=6465579

Family Applications (3)

Application Number Title Priority Date Filing Date
DE4226971A Expired - Lifetime DE4226971C2 (de) 1992-08-14 1992-08-14 Modifizierte Pilzzellen und Verfahren zur Herstellung rekombinanter Produkte
DE4244915A Expired - Lifetime DE4244915C2 (de) 1992-08-14 1992-08-14 DNA-Moleküle codierend Proteine, die an der O-Glykosylierung von Proteinen beteiligt sind
DE69333112T Expired - Lifetime DE69333112T2 (de) 1992-08-14 1993-08-16 Modifizierte pilzzellen und methode zur herstelllung rekombinanter produkte

Family Applications Before (2)

Application Number Title Priority Date Filing Date
DE4226971A Expired - Lifetime DE4226971C2 (de) 1992-08-14 1992-08-14 Modifizierte Pilzzellen und Verfahren zur Herstellung rekombinanter Produkte
DE4244915A Expired - Lifetime DE4244915C2 (de) 1992-08-14 1992-08-14 DNA-Moleküle codierend Proteine, die an der O-Glykosylierung von Proteinen beteiligt sind

Country Status (13)

Country Link
US (1) US5714377A (de)
EP (1) EP0658203B1 (de)
JP (1) JP3630424B2 (de)
AT (1) ATE245701T1 (de)
AU (1) AU679935B2 (de)
CA (1) CA2140894C (de)
DE (3) DE4226971C2 (de)
ES (1) ES2203619T3 (de)
FI (1) FI121075B (de)
NO (1) NO320349B1 (de)
NZ (1) NZ255350A (de)
WO (1) WO1994004687A1 (de)
ZA (1) ZA935915B (de)

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US5728553A (en) 1992-09-23 1998-03-17 Delta Biotechnology Limited High purity albumin and method of producing
JPH08173170A (ja) * 1994-05-25 1996-07-09 Kirin Brewery Co Ltd キャンディダ・ユティリス酵母の形質転換系、およびそれによる異種遺伝子の発現
CA2221271A1 (en) * 1995-05-18 1996-11-21 Genencor International, Inc. Expression of glycosyltransferase in aspergillus
GB9526733D0 (en) * 1995-12-30 1996-02-28 Delta Biotechnology Ltd Fusion proteins
GB9902000D0 (en) * 1999-01-30 1999-03-17 Delta Biotechnology Ltd Process
AU2007200859A1 (en) * 1999-01-30 2007-03-22 Novozymes Delta Limited Process
CA2405709A1 (en) * 2000-04-12 2001-10-25 Human Genome Sciences, Inc. Albumin fusion proteins
US6946134B1 (en) 2000-04-12 2005-09-20 Human Genome Sciences, Inc. Albumin fusion proteins
US20050100991A1 (en) * 2001-04-12 2005-05-12 Human Genome Sciences, Inc. Albumin fusion proteins
RU2003120093A (ru) * 2000-12-05 2005-01-27 Дзе Пенн Стейт Рисерч Фаундейшн (Us) Способы и композиции для высокоэффективного получения гетерологичных белков в дрожжах
US7507413B2 (en) 2001-04-12 2009-03-24 Human Genome Sciences, Inc. Albumin fusion proteins
US20050054051A1 (en) * 2001-04-12 2005-03-10 Human Genome Sciences, Inc. Albumin fusion proteins
US20060084794A1 (en) * 2001-04-12 2006-04-20 Human Genome Sciences, Inc. Albumin fusion proteins
US20050244931A1 (en) * 2001-04-12 2005-11-03 Human Genome Sciences, Inc. Albumin fusion proteins
CA2383556A1 (en) * 2001-05-16 2002-11-16 Pharmacia & Upjohn Company High-efficiency assay for protein mannosyl transferases
US7176278B2 (en) 2001-08-30 2007-02-13 Biorexis Technology, Inc. Modified transferrin fusion proteins
AU2002332041A1 (en) * 2001-10-05 2003-04-22 Human Genome Sciences, Inc. Albumin fusion proteins
ES2545090T3 (es) * 2001-12-21 2015-09-08 Human Genome Sciences, Inc. Proteínas de fusión de albúmina y GCSF
WO2005003296A2 (en) 2003-01-22 2005-01-13 Human Genome Sciences, Inc. Albumin fusion proteins
EP1463752A4 (de) * 2001-12-21 2005-07-13 Human Genome Sciences Inc Albuminfusionsproteine
EP1471933A1 (de) 2002-02-07 2004-11-03 Delta Biotechnology Limited Mit albumin fusionierte anti-angiogenese-peptide
NZ540743A (en) * 2002-12-20 2008-06-30 Unilever Plc Preparation of antifreeze protein
GB0329722D0 (en) 2003-12-23 2004-01-28 Delta Biotechnology Ltd Modified plasmid and use thereof
US9057061B2 (en) 2003-12-23 2015-06-16 Novozymes Biopharma Dk A/S Gene expression technique
GB0329681D0 (en) 2003-12-23 2004-01-28 Delta Biotechnology Ltd Gene expression technique
CA2628725A1 (en) 2005-11-15 2007-05-31 Glycofi, Inc. Production of glycoproteins with reduced o-glycosylation
AU2012203729B2 (en) * 2005-11-22 2014-01-30 Glycofi, Inc Production of glycoproteins with reduced O-glycosylation
EP1816201A1 (de) * 2006-02-06 2007-08-08 CSL Behring GmbH Modifizierter Koagulationsfaktor VIIa mit verbesserter 'half-life'-Stabiltät
WO2007132949A1 (ja) 2006-05-16 2007-11-22 National Institute Of Advanced Industrial Science And Technology タンパク質の高分泌生産方法
JP5102833B2 (ja) 2006-07-24 2012-12-19 バイオレクシス ファーマシューティカル コーポレーション エキセンディン融合タンパク質
GB0711424D0 (en) * 2007-06-13 2007-07-25 Novozymes Delta Ltd Recombinant transferrin mutants
CN101835801B (zh) 2007-08-08 2014-09-10 诺维信生物制药丹麦公司 转铁蛋白变体和偶联物
US8637435B2 (en) * 2007-11-16 2014-01-28 Merck Sharp & Dohme Corp. Eukaryotic cell display systems
US20100311122A1 (en) 2008-02-20 2010-12-09 Glycofi, Inc Vectors and yeast strains for protein production
CA2715212A1 (en) 2008-03-03 2009-09-11 Glycofi, Inc. Surface display of recombinant proteins in lower eukaryotes
WO2010019487A1 (en) * 2008-08-12 2010-02-18 Glycofi, Inc. Improved vectors and yeast strains for protein production: ca2+ atpase overexpression
US9120871B2 (en) 2009-01-23 2015-09-01 Novo Nordisk A/S Process for preparing FGF21 with low degree of O-glycosylation
EP2617733A1 (de) 2009-02-06 2013-07-24 Novozymes Biopharma DK A/S Aufreinigungsverfahren
KR101722961B1 (ko) 2009-02-11 2017-04-04 알부메딕스 에이/에스 알부민 변이체 및 접합체
US20120003695A1 (en) 2009-02-25 2012-01-05 Davidson Robert C Metabolic engineering of a galactose assimilation pathway in the glycoengineered yeast pichia pastoris
US8748380B2 (en) 2009-10-30 2014-06-10 Novozymes Biopharma Dk A/S Albumin variants
CN102725396A (zh) 2009-10-30 2012-10-10 默沙东公司 在缺乏二肽基氨肽酶活性的巴斯德毕赤酵母中生产治疗性蛋白的方法
MX2012009349A (es) * 2010-02-10 2013-02-11 Biocon Ltd Metodo para reducir glucosilacion de proteinas, procedimientos y proteinas de los mismos.
WO2011106389A1 (en) 2010-02-24 2011-09-01 Merck Sharp & Dohme Corp. Method for increasing n-glycosylation site occupancy on therapeutic glycoproteins produced in pichia pastoris
US10233228B2 (en) 2010-04-09 2019-03-19 Albumedix Ltd Albumin derivatives and variants
CN103080130B (zh) 2010-05-27 2016-08-17 默沙东公司 制备具有改进特性的抗体的方法
JP5885191B2 (ja) 2011-01-04 2016-03-15 国立研究開発法人産業技術総合研究所 糖鎖改変酵母及びそれを用いた糖タンパク質の製造方法
US8936918B2 (en) 2011-02-25 2015-01-20 Merck Sharp & Dohme Corp. Yeast strain for the production of proteins with modified O-glycosylation
EP2714732A4 (de) 2011-05-25 2014-12-10 Merck Sharp & Dohme Verfahren zur herstellung fc-haltiger polypeptide mit verbesserten eigenschaften
EP2780364A2 (de) 2011-11-18 2014-09-24 Eleven Biotherapeutics, Inc. Proteine mit verbesserter halbwertzeit und anderen eigenschaften
US9944691B2 (en) 2012-03-16 2018-04-17 Albumedix A/S Albumin variants
GB2512156A (en) 2012-11-08 2014-09-24 Novozymes Biopharma Dk As Albumin variants
EP3004156A1 (de) 2013-06-07 2016-04-13 Novo Nordisk A/S Verfahren zur herstellung von reifen insulinpolypeptiden
US20160376570A1 (en) 2013-07-04 2016-12-29 Novartis Ag O-Mannosyltransferase Deficient Filamentous Fungal Cells and Methods of Use Thereof
CN108064266A (zh) 2014-07-21 2018-05-22 格利科斯芬兰公司 在丝状真菌中具有哺乳动物样n-聚糖的糖蛋白的制备
MX2018001825A (es) 2015-08-20 2018-05-07 Albumedix As Variantes y conjugados de albumina.

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DE2862495D1 (en) * 1977-11-08 1989-05-24 Genentech Inc Plasmid for transforming bacterial host to render it capable of polypeptide expression
IT1203758B (it) * 1986-03-27 1989-02-23 Univ Roma Vettori di clonazione e di espressione di geni eterologhi in lieviti e lieviti trasformati con tali vettori
US4929553A (en) * 1987-05-29 1990-05-29 Canadian Patents & Development Ltd. Protease for specific processing of secreted proteins
FR2649991B2 (fr) * 1988-08-05 1994-03-04 Rhone Poulenc Sante Utilisation de derives stables du plasmide pkd1 pour l'expression et la secretion de proteines heterologues dans les levures du genre kluyveromyces
FR2655660B1 (fr) * 1989-12-11 1992-03-20 Rhone Poulenc Sante Nouveaux polypeptides, sequences d'adn permettant leur expression, procede de preparation et leur utilisation.

Also Published As

Publication number Publication date
NO320349B1 (no) 2005-11-28
ES2203619T3 (es) 2004-04-16
DE4226971A1 (de) 1994-02-17
NZ255350A (en) 1996-11-26
US5714377A (en) 1998-02-03
AU4948293A (en) 1994-03-15
NO950534L (no) 1995-04-05
FI950628L (fi) 1995-02-13
AU679935B2 (en) 1997-07-17
CA2140894C (en) 2010-03-02
EP0658203A1 (de) 1995-06-21
CA2140894A1 (en) 1994-03-03
DE69333112T2 (de) 2004-06-09
JP3630424B2 (ja) 2005-03-16
FI121075B (fi) 2010-06-30
NO950534D0 (no) 1995-02-13
FI950628A0 (fi) 1995-02-13
DE4226971C2 (de) 1997-01-16
JPH08500975A (ja) 1996-02-06
EP0658203B1 (de) 2003-07-23
ZA935915B (en) 1995-04-13
WO1994004687A1 (en) 1994-03-03
ATE245701T1 (de) 2003-08-15
DE4244915C2 (de) 1998-12-03

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Legal Events

Date Code Title Description
8332 No legal effect for de
8370 Indication related to discontinuation of the patent is to be deleted
8364 No opposition during term of opposition
8328 Change in the person/name/address of the agent

Representative=s name: STROHSCHAENK UND KOLLEGEN, 85521 OTTOBRUNN

8327 Change in the person/name/address of the patent owner

Owner name: NOVOZYMES BIOPHARMA DK A/S, BAGSVAERD, DK