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ES2150905T3 - Hidrolisis enzimatica del almidon a la glucosa con la ayuda de una enzima producida por ingenieria genetica. - Google Patents

Hidrolisis enzimatica del almidon a la glucosa con la ayuda de una enzima producida por ingenieria genetica.

Info

Publication number
ES2150905T3
ES2150905T3 ES91912567T ES91912567T ES2150905T3 ES 2150905 T3 ES2150905 T3 ES 2150905T3 ES 91912567 T ES91912567 T ES 91912567T ES 91912567 T ES91912567 T ES 91912567T ES 2150905 T3 ES2150905 T3 ES 2150905T3
Authority
ES
Spain
Prior art keywords
starch
enzyme
glucose
help
genetic engineering
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES91912567T
Other languages
English (en)
Inventor
Karin Birte Svensson
Michael Richard Sierks
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novo Nordisk AS
Original Assignee
Novo Nordisk AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novo Nordisk AS filed Critical Novo Nordisk AS
Application granted granted Critical
Publication of ES2150905T3 publication Critical patent/ES2150905T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/20—Preparation of compounds containing saccharide radicals produced by the action of an exo-1,4 alpha-glucosidase, e.g. dextrose

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Saccharide Compounds (AREA)

Abstract

SE PRESENTA UN PROCESO PARA CONVERTIR ALMIDON O ALMIDON PARCIALMENTE HIDROLIZADO EN UN SIROPE QUE CONTIENE DEXTROSA. EL PROCESO INCLUYE LOS PASOS DE SACARIFICAR HIDROCILATO DE ALMIDON EN LA PRESENCIA DE UNA GLUCOAMILASA MUTADA O ENZIMA RELACIONADA E INCREMENTAR LA SELECTIVIDAD DE LA ENZIMA PARA LOS ENLACES (ALFA)-(14)LACIONADA INCLUYENDO AL MENOS UNA MUTACION, SUBSTITUYENDO LA MUTACION A UN AMINOACIDO DE LA ENZIMA CON AL MENOS UN AMINOACIDO ESCOGIDO POR COMPARACION CON LAS REGIONES RELACIONADAS ESTRUCTURALMENTE DE OTRAS ENZIMAS QUE HIDROLICEN SELECTIVAMENTE LOS ENLACES (ALFA)-(1-4)GLUCOSIDICOS SOLAMENTE. TAMBIEN SE PRESENTAN ENZIMAS FABRICADAS DE ACUERDO CON LA INVENCION.
ES91912567T 1990-06-29 1991-06-28 Hidrolisis enzimatica del almidon a la glucosa con la ayuda de una enzima producida por ingenieria genetica. Expired - Lifetime ES2150905T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/546,511 US5162210A (en) 1990-06-29 1990-06-29 Process for enzymatic hydrolysis of starch to glucose

Publications (1)

Publication Number Publication Date
ES2150905T3 true ES2150905T3 (es) 2000-12-16

Family

ID=24180763

Family Applications (1)

Application Number Title Priority Date Filing Date
ES91912567T Expired - Lifetime ES2150905T3 (es) 1990-06-29 1991-06-28 Hidrolisis enzimatica del almidon a la glucosa con la ayuda de una enzima producida por ingenieria genetica.

Country Status (10)

Country Link
US (1) US5162210A (es)
EP (1) EP0536270B1 (es)
JP (1) JP3249514B2 (es)
AT (1) ATE195972T1 (es)
DE (1) DE69132391T2 (es)
DK (1) DK0536270T3 (es)
ES (1) ES2150905T3 (es)
FI (1) FI107807B (es)
GR (1) GR3034781T3 (es)
WO (1) WO1992000381A1 (es)

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WO2024227153A1 (en) 2023-04-28 2024-10-31 International N&H Denmark Aps Ruminant feed additive compositions
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FI107807B (fi) 2001-10-15
DK0536270T3 (da) 2000-10-30
FI925901L (fi) 1992-12-28
GR3034781T3 (en) 2001-02-28
ATE195972T1 (de) 2000-09-15
US5162210A (en) 1992-11-10
DE69132391D1 (de) 2000-10-05
FI925901A0 (fi) 1992-12-28
WO1992000381A1 (en) 1992-01-09
JPH05507852A (ja) 1993-11-11
EP0536270A1 (en) 1993-04-14
DE69132391T2 (de) 2001-02-15
JP3249514B2 (ja) 2002-01-21
EP0536270B1 (en) 2000-08-30

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