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EA201691834A1 - Новый способ очистки гонадотропина - Google Patents

Новый способ очистки гонадотропина

Info

Publication number
EA201691834A1
EA201691834A1 EA201691834A EA201691834A EA201691834A1 EA 201691834 A1 EA201691834 A1 EA 201691834A1 EA 201691834 A EA201691834 A EA 201691834A EA 201691834 A EA201691834 A EA 201691834A EA 201691834 A1 EA201691834 A1 EA 201691834A1
Authority
EA
Eurasian Patent Office
Prior art keywords
purification
gonadotropin
target
chromatography
gonadotropine
Prior art date
Application number
EA201691834A
Other languages
English (en)
Inventor
Санджив Кумар Мендиратта
Санджай Бандиопадхиай
Аваниш К. Сингх
Митхра С. Редди
Original Assignee
Кадила Хелзкэр Лимитед
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Кадила Хелзкэр Лимитед filed Critical Кадила Хелзкэр Лимитед
Publication of EA201691834A1 publication Critical patent/EA201691834A1/ru

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/59—Follicle-stimulating hormone [FSH]; Chorionic gonadotropins, e.g.hCG [human chorionic gonadotropin]; Luteinising hormone [LH]; Thyroid-stimulating hormone [TSH]
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/32—Bonded phase chromatography
    • B01D15/325—Reversed phase
    • B01D15/327—Reversed phase with hydrophobic interaction
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361—Ion-exchange
    • B01D15/363—Anion-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3804—Affinity chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Endocrinology (AREA)
  • Reproductive Health (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Engineering & Computer Science (AREA)
  • Water Supply & Treatment (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)

Abstract

В настоящем изобретении предлагается усовершенствованный способ очистки целевого гонадотропина из неочищенной смеси, содержащей по меньшей мере один загрязняющий белок. Способ очистки целевого гонадотропина согласно настоящему изобретению включает применение аффинной хроматографии в качестве первой стадии колоночной очистки перед применением каких-либо стадий колоночной хроматографии для дополнительной очистки. Такой способ очистки может дополнительно включать стадию ионообменной хроматографии и/или хроматографии гидрофобного взаимодействия с получением по существу очищенного белка гонадотропина белка с целевым профилем изоформ.
EA201691834A 2014-04-18 2015-04-17 Новый способ очистки гонадотропина EA201691834A1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IN1398MU2014 2014-04-18
PCT/IN2015/000175 WO2015159309A1 (en) 2014-04-18 2015-04-17 Novel purification process of gonadotropin

Publications (1)

Publication Number Publication Date
EA201691834A1 true EA201691834A1 (ru) 2017-02-28

Family

ID=53773477

Family Applications (1)

Application Number Title Priority Date Filing Date
EA201691834A EA201691834A1 (ru) 2014-04-18 2015-04-17 Новый способ очистки гонадотропина

Country Status (15)

Country Link
US (1) US20170029482A1 (ru)
EP (1) EP3131924A1 (ru)
JP (1) JP2017514803A (ru)
KR (1) KR20160131113A (ru)
CN (1) CN106170493A (ru)
AR (1) AR100131A1 (ru)
AU (1) AU2015248461B2 (ru)
BR (1) BR112016023680A2 (ru)
CA (1) CA2945591A1 (ru)
EA (1) EA201691834A1 (ru)
IL (1) IL248293A (ru)
MX (1) MX2016013416A (ru)
MY (1) MY183011A (ru)
SG (1) SG11201608513WA (ru)
WO (1) WO2015159309A1 (ru)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105968185A (zh) * 2016-07-20 2016-09-28 宁波人健药业集团股份有限公司 一种绒促性素纯化方法
CN108503705A (zh) * 2018-07-10 2018-09-07 北京伟杰信生物科技有限公司 一种重组绒促性素（rhCG）的纯化方法
KR102752764B1 (ko) * 2019-12-26 2025-01-14 주식회사 엘지화학 여포 자극 호르몬의 정제 방법
CN111303274B (zh) * 2020-03-21 2024-01-30 上海浦东明炎生物技术有限公司 一种人绒毛膜促性腺激素纯化方法
CN114591414A (zh) * 2022-03-24 2022-06-07 江西浩然生物制药有限公司 一种人绒毛膜促性腺激素的制备方法
CN116143901B (zh) * 2022-11-28 2024-08-02 景泽生物医药(合肥)股份有限公司 一种促卵泡激素的纯化方法

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005006381A1 (ja) 2003-07-15 2005-01-20 Matsushita Electric Industrial Co., Ltd. プラズマディスプレイパネルの製造方法
ES2333128T3 (es) * 2003-12-22 2010-02-17 Ares Trading S.A. Metodo para purificar fsh.
PT1809663E (pt) 2004-11-09 2008-10-02 Ares Trading Sa Um cesto para cultivo de marisco
US8604175B2 (en) * 2005-12-09 2013-12-10 Ares Trading S.A. Method for purifying FSH or a FSH mutant
NZ595661A (en) * 2009-04-01 2013-07-26 Method for purifying recombinant follicle stimulating hormone (fsh)
EP2325194A1 (en) * 2009-11-24 2011-05-25 Glycotope GmbH Process for the purification of glycoproteins
CN102464713A (zh) * 2010-12-21 2012-05-23 上海丽珠制药有限公司 一种卵泡刺激素的制备方法
CN103059125B (zh) * 2012-12-27 2015-08-12 浙江海正药业股份有限公司 重组人促卵泡激素的纯化方法

Also Published As

Publication number Publication date
WO2015159309A1 (en) 2015-10-22
MX2016013416A (es) 2017-01-18
CA2945591A1 (en) 2015-10-22
EP3131924A1 (en) 2017-02-22
MY183011A (en) 2021-02-05
JP2017514803A (ja) 2017-06-08
AU2015248461B2 (en) 2017-05-04
AU2015248461A1 (en) 2016-11-03
KR20160131113A (ko) 2016-11-15
IL248293A (en) 2017-04-30
BR112016023680A2 (pt) 2017-08-15
US20170029482A1 (en) 2017-02-02
SG11201608513WA (en) 2016-11-29
CN106170493A (zh) 2016-11-30
AR100131A1 (es) 2016-09-14

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