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EA202090262A1 - Очистка белка - Google Patents

Очистка белка

Info

Publication number
EA202090262A1
EA202090262A1 EA202090262A EA202090262A EA202090262A1 EA 202090262 A1 EA202090262 A1 EA 202090262A1 EA 202090262 A EA202090262 A EA 202090262A EA 202090262 A EA202090262 A EA 202090262A EA 202090262 A1 EA202090262 A1 EA 202090262A1
Authority
EA
Eurasian Patent Office
Prior art keywords
purification
protein
relates
chromatography step
protein cleaning
Prior art date
Application number
EA202090262A
Other languages
English (en)
Inventor
Майкл Гарри Роуз
Original Assignee
Юсб Биофарма Срл
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Юсб Биофарма Срл filed Critical Юсб Биофарма Срл
Publication of EA202090262A1 publication Critical patent/EA202090262A1/ru

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/18—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns
    • B01D15/1814—Recycling of the fraction to be distributed
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/24—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the treatment of the fractions to be distributed
    • B01D15/245—Adding materials to the effluents
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/24—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the treatment of the fractions to be distributed
    • B01D15/247—Fraction collectors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3804—Affinity chromatography
    • B01D15/3809—Affinity chromatography of the antigen-antibody type, e.g. protein A, G or L chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Water Supply & Treatment (AREA)
  • Sustainable Development (AREA)
  • Peptides Or Proteins (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)

Abstract

Изобретение относится к области очистки, и в частности очистки белка. В изобретении предложены улучшенные способы очистки белков и других биомолекул в промышленных масштабах. Более конкретно, оно относится к способу очистки представляющего интерес соединения, такого как белок, предпочтительно антитело или фрагмент антитела, с помощью стадии хроматографии, предпочтительно стадии полунепрерывной хроматографии.
EA202090262A 2017-07-17 2018-07-16 Очистка белка EA202090262A1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB1711481.0A GB201711481D0 (en) 2017-07-17 2017-07-17 Protein purification
PCT/EP2018/069298 WO2019016154A1 (en) 2017-07-17 2018-07-16 Chromatography

Publications (1)

Publication Number Publication Date
EA202090262A1 true EA202090262A1 (ru) 2020-05-12

Family

ID=59713705

Family Applications (1)

Application Number Title Priority Date Filing Date
EA202090262A EA202090262A1 (ru) 2017-07-17 2018-07-16 Очистка белка

Country Status (16)

Country Link
US (1) US11498941B2 (ru)
EP (1) EP3655420A1 (ru)
JP (1) JP7145196B2 (ru)
KR (1) KR102542274B1 (ru)
CN (1) CN110945008A (ru)
AU (1) AU2018304867B2 (ru)
BR (1) BR112019027760A2 (ru)
CA (1) CA3067496A1 (ru)
CL (1) CL2020000087A1 (ru)
CO (1) CO2020000090A2 (ru)
EA (1) EA202090262A1 (ru)
GB (1) GB201711481D0 (ru)
IL (1) IL271790B2 (ru)
MX (1) MX2020000288A (ru)
SG (1) SG11201911880UA (ru)
WO (1) WO2019016154A1 (ru)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201916961D0 (en) * 2019-11-21 2020-01-08 Puridify Ltd Method in bioprocess purification system
CN115087861A (zh) * 2020-02-13 2022-09-20 豪夫迈·罗氏有限公司 用于靶标化合物纯化的方法/装置
EP4083053A1 (en) * 2021-04-30 2022-11-02 Fresenius Kabi iPSUM S.r.l. Method for icatibant preparation
US20250130209A1 (en) * 2021-08-13 2025-04-24 Blutech Aps Industrial method for isolating an analyte from a liquid mixture
TW202337900A (zh) * 2022-01-12 2023-10-01 美商再生元醫藥公司 改良使用親和層析的異二聚蛋白質自雜質之解析的方法
US20250206775A1 (en) * 2022-03-18 2025-06-26 Bristol-Myers Squibb Company Methods of isolating polypeptides
WO2024056626A1 (en) * 2022-09-13 2024-03-21 Sanofi Aventis Deutschland Gmbh Continuous gradient elution chromatographic fractionation
WO2024137836A1 (en) * 2022-12-22 2024-06-27 National Resilience, Inc. Physical integration device for on-line purification analytics

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5940161A (ja) 1982-08-30 1984-03-05 Asahi Chem Ind Co Ltd カラムクロマトグラフイ−による分取方法
JPS61270658A (ja) 1985-05-25 1986-11-29 Hitachi Plant Eng & Constr Co Ltd 分離精製装置
DE102005026486A1 (de) * 2005-06-09 2006-12-14 Bayer Technology Services Gmbh Ein chromatographisches quasi-kontinuierliches Verfahren und entsprechende Vorrichtung zur Trennung von binären und Mehrstoffgemischen
US8569464B2 (en) * 2006-12-21 2013-10-29 Emd Millipore Corporation Purification of proteins
BR112014011304B1 (pt) 2011-11-11 2022-03-03 UCB Biopharma SRL Anticorpos de ligação com albumina e fragmentos de ligação dos mesmos, proteína de fusão de anticorpo bi-específico, polinucleotídeo, célula hospedeira, processo de produção de anticorpo ou fragamento do mesmo, formulação farmacêutica e uso
EP3492486A1 (en) 2011-12-22 2019-06-05 F. Hoffmann-La Roche AG Ion exchange membrane chromatography
KR20150129033A (ko) 2013-03-14 2015-11-18 애브비 인코포레이티드 저 산성 종 조성물 및 이의 제조 및 사용 방법
RU2662668C2 (ru) * 2013-05-06 2018-07-26 Санофи Способ непрерывной многостадийной очистки антител
GB2528301A (en) * 2014-07-17 2016-01-20 Univ Newcastle Biomolecule separation using apatite
GB201602938D0 (en) 2016-02-19 2016-04-06 Ucb Biopharma Sprl Protein purification

Also Published As

Publication number Publication date
KR20200027552A (ko) 2020-03-12
AU2018304867B2 (en) 2025-05-22
WO2019016154A1 (en) 2019-01-24
EP3655420A1 (en) 2020-05-27
SG11201911880UA (en) 2020-01-30
US20200331960A1 (en) 2020-10-22
CN110945008A (zh) 2020-03-31
JP2020527553A (ja) 2020-09-10
BR112019027760A2 (pt) 2020-07-07
AU2018304867A1 (en) 2020-01-02
US11498941B2 (en) 2022-11-15
JP7145196B2 (ja) 2022-09-30
IL271790B2 (en) 2024-09-01
CA3067496A1 (en) 2019-01-24
CO2020000090A2 (es) 2020-04-24
IL271790A (en) 2020-02-27
IL271790B1 (en) 2024-05-01
MX2020000288A (es) 2020-07-22
GB201711481D0 (en) 2017-08-30
KR102542274B1 (ko) 2023-06-12
CL2020000087A1 (es) 2020-07-24

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