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DK2678352T3 - Polypeptider med cellulolyseforbedrende aktivitet og polynukleotider, som koder for dem - Google Patents

Polypeptider med cellulolyseforbedrende aktivitet og polynukleotider, som koder for dem Download PDF

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Publication number
DK2678352T3
DK2678352T3 DK12749154.6T DK12749154T DK2678352T3 DK 2678352 T3 DK2678352 T3 DK 2678352T3 DK 12749154 T DK12749154 T DK 12749154T DK 2678352 T3 DK2678352 T3 DK 2678352T3
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polypeptide
seq
acid
preferred aspect
fermentation
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DK12749154.6T
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Yu Zhang
Junxin Duan
Lan Tang
Wenping Wu
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Novozymes Inc
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    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
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    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
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    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
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    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
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    • C12P7/00—Preparation of oxygen-containing organic compounds
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    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
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    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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    • Y02P20/00—Technologies relating to chemical industry
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  • Proteomics, Peptides & Aminoacids (AREA)
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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Claims (14)

1. Isoleret poiypeptid med celfuloiyseforbed rende aktivitet, som er vaigt fra gruppen bestående af et poiypeptid, der udviser mindst 90% sekvensidentitet med det modne polypeptid ifølge SEQ ID NO: 2.
2. Poiypeptid ifølge krav 1, som omfatter eller består af aminosyresekvensen ifølge SEQ ID NO: 2 eller det modne poiypeptid ifølge SEQ ID NO: 2; eller et fragment deraf med cellulolyseforbedrende aktivitet,
3. Polypeptid ifølge krav 1, som kodes for af poiynukleotidet indeholdt i plasmid pGH6f J364, hvilket plasmid er Indeholdt i E, coll CGMCC 4601,
4. Isoleret polynukleotid, som koder for polypeptidet ifølge et hvilket som helst af kravene 1-3.
5. Fremgangsmåde til fremstilling af et polypeptid med cellulolyseforbedrende aktivitet, hvilken fremgangsmåde omfatter: (a) dyrkning afen rekombinant værtscelle, som omfatter poiynukleotidet ifølge krav 4, under betingelser, der er befordrende for fremstiiling af polypeptidet; og (b) indvinding af polypeptidet.
6. Sammensætning, som omfatter polypeptidet ifølge et hviiket som helst af kravene 1-3.
7. Sammensætning ifølge krav 6, som er en celluiolytisk enzymsam mensætning eller en detergentsammensætning.
8. Fremgangsmåde til nedbrydning eller omdannelse af et cetluloseholdigt materiale, hvilken fremgangsmåde omfatter: behandling af det ceiluloseholdige materiale med en enzymsammensætning i nærværelse af polypeptidet med ceilulolyseforbedrende aktivitet ifølge et hvilket som helst af kravene 1-3.
9. Fremgangsmåde ifølge krav 8, som endvidere omfatter indvinding af det nedbrudte ceiluloseholdige materiale.
10. Fremgangsmåde til fremstilling af et fermenteringsprodukt, hviiken fremgangsmåde omfatter: (a) forsukring af et celluloseholdigt materiale med en enzymsammensætning i nærværelse af polypeptidet med cellulolyseforbedrende aktivitet ifølge et hvilket som helst af kravene 1-3; (b) fermentering af det forsukrede ceiiuioseholdige materiale med en eller flere {f.eks. adskillige) fermenterende mikroorganismer for at fremstille fermenteringsproduktet; og (c) indvinding af fermenteringsproduktet fra fermenteringen.
11. Fremgangsmåde til fermentering af et ceiiuloseholdigt materiale, hvilken fremgangsmåde omfatter: forsukring af det ceiiuioseholdige materiale med en enzymsammensætning i nærværelse af et polypeptid med cellulofyseforbedrende aktivitet ifølge et hvilket som heist af kravene 1*3 og fermentering af det ceiiuioseholdige materiale med en eiSer flere {f.eks, adskillige) fermenterende mikroorganismer.
12. Fremgangsmåde ifølge krav 11, hvor fermenteringen af det ceituloseholdige materiale frembringer et fermenteringsprodukt.
13. Fremgangsmåde ifølge krav 12, som endvidere omfatter indvinding af fermenteringsproduktet fra fermenteringen.
14. Totalmedieformuiering eller celledyrkningssammensætning, som omfatter polypeptidet ifølge et hvilket som helst af kravene 1 -3.
DK12749154.6T 2011-02-23 2012-02-23 Polypeptider med cellulolyseforbedrende aktivitet og polynukleotider, som koder for dem DK2678352T3 (da)

Applications Claiming Priority (2)

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CN2011071208 2011-02-23
PCT/CN2012/071525 WO2012113340A1 (en) 2011-02-23 2012-02-23 Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same

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CN (1) CN103384678B (da)
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