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DE60234247D1 - Verfahren zur sekretion und produktion von protein - Google Patents

Verfahren zur sekretion und produktion von protein

Info

Publication number
DE60234247D1
DE60234247D1 DE60234247T DE60234247T DE60234247D1 DE 60234247 D1 DE60234247 D1 DE 60234247D1 DE 60234247 T DE60234247 T DE 60234247T DE 60234247 T DE60234247 T DE 60234247T DE 60234247 D1 DE60234247 D1 DE 60234247D1
Authority
DE
Germany
Prior art keywords
coryneform bacterium
heterologous protein
protein
production
sequence encoding
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60234247T
Other languages
English (en)
Inventor
Yoshimi Kikuchi
Masayo Date
Yukiko Umezawa
Keiichi Yokoyama
Haruo Heima
Hiroshi Matsui
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ajinomoto Co Inc
Original Assignee
Ajinomoto Co Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ajinomoto Co Inc filed Critical Ajinomoto Co Inc
Application granted granted Critical
Publication of DE60234247D1 publication Critical patent/DE60234247D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/36—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Actinomyces; from Streptomyces (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/34—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Corynebacterium (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
    • C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
    • C07K5/10—Tetrapeptides
    • C07K5/1002—Tetrapeptides with the first amino acid being neutral
    • C07K5/1016—Tetrapeptides with the first amino acid being neutral and aromatic or cycloaliphatic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
DE60234247T 2001-03-30 2002-03-27 Verfahren zur sekretion und produktion von protein Expired - Lifetime DE60234247D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2001098808 2001-03-30
PCT/JP2002/002978 WO2002081694A1 (fr) 2001-03-30 2002-03-27 Procede de secretion et de production de proteine

Publications (1)

Publication Number Publication Date
DE60234247D1 true DE60234247D1 (de) 2009-12-17

Family

ID=18952423

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60234247T Expired - Lifetime DE60234247D1 (de) 2001-03-30 2002-03-27 Verfahren zur sekretion und produktion von protein

Country Status (14)

Country Link
US (1) US7252972B2 (de)
EP (1) EP1375664B1 (de)
JP (1) JP4362651B2 (de)
KR (1) KR100602807B1 (de)
CN (1) CN1250722C (de)
AT (1) ATE447615T1 (de)
AU (1) AU2002242986A1 (de)
BR (1) BR0208136B1 (de)
CA (1) CA2442679C (de)
DE (1) DE60234247D1 (de)
DK (1) DK1375664T3 (de)
ES (1) ES2335750T3 (de)
RU (1) RU2264463C2 (de)
WO (1) WO2002081694A1 (de)

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US7723081B1 (en) 2000-01-21 2010-05-25 Ajinomoto Co., Inc. Bacteria containing aspartate-semialdehyde dehydrogenase, phosphoenolpyruvate carboxylase, and transhydrogenase to produce L-lysine in escherichia, and methods of using same
CA2518049C (en) * 2003-03-07 2013-05-07 Ajinomoto Co., Inc. Method of producing microbial transglutaminase
DE10359660A1 (de) * 2003-12-18 2005-07-28 Basf Ag Psod-Expressionseinheiten
DE10359594A1 (de) * 2003-12-18 2005-07-28 Basf Ag PEF-TU-Expressionseinheiten
JP4730302B2 (ja) * 2004-04-20 2011-07-20 味の素株式会社 タンパク質の製造法
WO2005121333A1 (en) * 2004-06-14 2005-12-22 Novozymes A/S Signal peptide for producing a polypeptide
US7527947B2 (en) 2004-06-14 2009-05-05 Novozymes A/S Signal peptide for producing a polypeptide
DE102004035065A1 (de) 2004-07-20 2006-02-16 Basf Ag P-ET-TS-Expressionseinheiten
KR100620092B1 (ko) * 2004-12-16 2006-09-08 씨제이 주식회사 코리네박테리움 속 세포로부터 유래된 신규한 프로모터서열, 그를 포함하는 발현 카세트 및 벡터, 상기 벡터를포함하는 숙주 세포 및 그를 이용하여 유전자를 발현하는방법
EP1902126A1 (de) * 2005-07-12 2008-03-26 Codon Devices, Inc Zusammensetzungen und verfahren für biokatalytische manipulation
RU2338784C2 (ru) * 2006-03-24 2008-11-20 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ) Новая альдолаза, днк, кодирующая альдолазу, клетки, трансформированные днк, способ получения альдолазы и способ получения 4-гидрокси-l-изолейцина (варианты)
EP2082045B1 (de) * 2006-10-24 2015-03-18 Basf Se Verfahren zur reduzierung der genexpression mittels modifizierter codonverwendung
JPWO2008099898A1 (ja) * 2007-02-15 2010-05-27 味の素株式会社 ジスルフィド結合導入トランスグルタミナーゼ
WO2008120798A1 (ja) 2007-03-29 2008-10-09 Ajinomoto Co., Inc. 接着用酵素製剤及び接着成形食品の製造方法
EP2213785A4 (de) 2007-11-19 2013-01-09 Ajinomoto Kk Verarbeitetes faserprodukt und herstellungsverfahren dafür
EP2302066A4 (de) * 2008-07-09 2012-02-08 Ajinomoto Kk Verfahren zur herstellung von aminohydroxybenzoesäure
TWI444146B (zh) 2008-09-25 2014-07-11 Ajinomoto Kk 黏著成型食品用酵素製劑及黏著成型食品之製造方法
CN102341497B (zh) 2009-03-06 2013-11-06 味之素株式会社 放线菌来源的耐热性转谷氨酰胺酶
MY160554A (en) 2010-02-23 2017-03-15 Toray Industries Process for production of cadaverine
CN103917656B (zh) 2011-10-25 2016-09-28 味之素株式会社 蛋白质的分泌产生方法
AU2012333451B2 (en) 2011-11-02 2015-05-21 Ajinomoto Co., Inc. Method for secretory production of protein
AU2012333515B2 (en) 2011-11-02 2015-07-16 Ajinomoto Co., Inc. Method for secreting and producing proteins
AU2013240184B2 (en) 2012-03-26 2017-05-25 Axcella Health Inc. Nutritive fragments, proteins and methods
EP2831100A4 (de) 2012-03-26 2016-02-10 Pronutria Inc Nährstoffproteine und verfahren
SG10201604464SA (en) 2012-03-26 2016-07-28 Axcella Health Inc Charged nutritive proteins and methods
CA2868469A1 (en) 2012-03-26 2013-10-03 Pronutria, Inc. Nutritive fragments, proteins and methods
AU2014324901A1 (en) 2013-09-25 2016-05-19 Axcella Health Inc. Compositions and formulations for prevention and treatment of diabetes and obesity, and methods of production and use thereof in glucose and caloric control
EP3287523B1 (de) 2015-04-24 2022-06-29 Ajinomoto Co., Inc. Verfahren zur sekretorischen herstellung eines proteins
EP3375883B1 (de) 2015-11-12 2021-02-03 Ajinomoto Co., Inc. Verfahren zur herstellung von n-epsilon-acyl-l-lysin
JP7020403B2 (ja) 2016-05-02 2022-02-16 味の素株式会社 アジド基含有Ｆｃタンパク質
US11242545B2 (en) 2016-09-01 2022-02-08 Ningxia Eppen Biotech Co., Ltd Corynebacterium for producing L-lysine by fermentation
JP7021639B2 (ja) 2016-10-21 2022-02-17 味の素株式会社 タンパク質の分泌生産法
EP3530747A4 (de) 2016-10-21 2020-07-22 Ajinomoto Co., Inc. Verfahren zur herstellung von proteinsekreten
EP3572522A4 (de) 2017-01-19 2020-10-28 Ajinomoto Co., Inc. Verfahren zur herstellung einer heparosanverbindung mit isomerisiertem hexulonsäurerest
EP3415622A1 (de) 2017-06-14 2018-12-19 Evonik Degussa GmbH Verfahren zur herstellung von feinchemikalien mittels eines corynebakteriums, welches modifizierte alpha-1,6-glucosidasen sezerniert
JP7200948B2 (ja) 2017-09-05 2023-01-10 味の素株式会社 ２－ｏ－硫酸化酵素変異体、および３－ｏ－硫酸化酵素変異体、ならびにそれらを用いる方法
JP7439749B2 (ja) 2018-04-20 2024-02-28 味の素株式会社 タンパク質の分泌生産法
WO2020085511A1 (ja) 2018-10-25 2020-04-30 味の素株式会社 タンパク質の分泌生産法
JPWO2020090979A1 (ja) 2018-10-31 2021-09-24 味の素株式会社 抗体に対する親和性物質、切断性部分および反応性基を有する化合物またはその塩
EP4115740A4 (de) 2020-03-04 2024-04-03 Ajinomoto Co., Inc. Mutante transglutaminase
WO2022071061A1 (ja) 2020-09-29 2022-04-07 味の素株式会社 変異型トランスグルタミナーゼ
KR20240032941A (ko) 2021-07-07 2024-03-12 아지노모토 가부시키가이샤 비천연 아미노산 함유 단백질의 분비 생산법
JPWO2023200008A1 (de) 2022-04-15 2023-10-19
EP4534547A1 (de) 2022-06-02 2025-04-09 Ajinomoto Co., Inc. Affinitätssubstanz, verbindung und antikörper sowie deren salze

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Publication number Priority date Publication date Assignee Title
DE3584848D1 (de) * 1984-04-04 1992-01-23 Ajinomoto Kk Zusammengesetztes transduzierbares plasmid.
JPH07112431B2 (ja) * 1985-09-06 1995-12-06 味の素株式会社 遺伝子発現調節法
RU2091490C1 (ru) * 1985-10-25 1997-09-27 Зимодженетикс, Инк. Способ получения гетерологичного полипептида в эукариотических микроорганизмов
US4965197A (en) * 1987-06-12 1990-10-23 Massachusetts Institute Of Technology Coryneform expression and secretion system
WO1988009821A1 (en) * 1987-06-12 1988-12-15 Massachusetts Institute Of Technology Coryneform expression and secretion system
EP0551506A1 (de) * 1991-07-30 1993-07-21 Orsan Expressionssystem und Sekretion von Proteinen brauchbar insbesondere in Corynebakterien.
KR0164235B1 (ko) * 1993-01-13 1999-01-15 도바 다다스 신규한 세포 표층 단백질
JP3711658B2 (ja) 1996-10-07 2005-11-02 味の素株式会社 コリネバクテリウム・アンモニアゲネス由来の新規な細胞表層蛋白質
EP0897003B1 (de) * 1997-07-05 2004-05-12 Societe Des Produits Nestle S.A. Verstärkte Expression von proteolytischen Enzymen in Koji Pilz
JPH11169182A (ja) * 1997-12-10 1999-06-29 Mitsubishi Chemical Corp コリネ型細菌内で蛋白質を効率よく培地中へ分泌させる変異型分泌装置遺伝子
WO2001023591A1 (fr) * 1999-09-30 2001-04-05 Ajinomoto Co., Inc. Procédé de production de transglutaminase

Also Published As

Publication number Publication date
EP1375664B1 (de) 2009-11-04
US7252972B2 (en) 2007-08-07
JP4362651B2 (ja) 2009-11-11
KR20030087042A (ko) 2003-11-12
KR100602807B1 (ko) 2006-07-20
CN1500145A (zh) 2004-05-26
BR0208136A (pt) 2004-03-02
RU2264463C2 (ru) 2005-11-20
JPWO2002081694A1 (ja) 2004-07-29
BR0208136B1 (pt) 2014-10-21
WO2002081694A1 (fr) 2002-10-17
WO2002081694A8 (fr) 2002-11-14
ATE447615T1 (de) 2009-11-15
CN1250722C (zh) 2006-04-12
CA2442679C (en) 2010-06-01
EP1375664A1 (de) 2004-01-02
RU2003128977A (ru) 2005-02-27
ES2335750T3 (es) 2010-04-05
CA2442679A1 (en) 2002-10-17
AU2002242986A1 (en) 2002-10-21
EP1375664A4 (de) 2005-08-17
US20040126847A1 (en) 2004-07-01
DK1375664T3 (da) 2010-02-08

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