[go: up one dir, main page]

ATE532870T1 - Verfahren zur herstellung von transglutaminase - Google Patents

Verfahren zur herstellung von transglutaminase

Info

Publication number
ATE532870T1
ATE532870T1 AT00962986T AT00962986T ATE532870T1 AT E532870 T1 ATE532870 T1 AT E532870T1 AT 00962986 T AT00962986 T AT 00962986T AT 00962986 T AT00962986 T AT 00962986T AT E532870 T1 ATE532870 T1 AT E532870T1
Authority
AT
Austria
Prior art keywords
transglutaminase
coryneform bacterium
foreign protein
pro
secretory production
Prior art date
Application number
AT00962986T
Other languages
English (en)
Inventor
Yoshimi Kikuchi
Masayo Date
Yukiko Umezawa
Keiichi Yokoyama
Hiroshi Matsui
Original Assignee
Ajinomoto Kk
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ajinomoto Kk filed Critical Ajinomoto Kk
Application granted granted Critical
Publication of ATE532870T1 publication Critical patent/ATE532870T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/104—Aminoacyltransferases (2.3.2)
    • C12N9/1044—Protein-glutamine gamma-glutamyltransferase (2.3.2.13), i.e. transglutaminase or factor XIII
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/02—Aminoacyltransferases (2.3.2)
    • C12Y203/02013—Protein-glutamine gamma-glutamyltransferase (2.3.2.13), i.e. transglutaminase or factor XIII
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AT00962986T 1999-09-30 2000-09-29 Verfahren zur herstellung von transglutaminase ATE532870T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP28009899 1999-09-30
JP2000194043 2000-06-28
PCT/JP2000/006780 WO2001023591A1 (fr) 1999-09-30 2000-09-29 Procédé de production de transglutaminase

Publications (1)

Publication Number Publication Date
ATE532870T1 true ATE532870T1 (de) 2011-11-15

Family

ID=26553616

Family Applications (1)

Application Number Title Priority Date Filing Date
AT00962986T ATE532870T1 (de) 1999-09-30 2000-09-29 Verfahren zur herstellung von transglutaminase

Country Status (12)

Country Link
US (2) US7723067B2 (de)
EP (1) EP1219713B1 (de)
JP (1) JP4320769B2 (de)
KR (1) KR100565030B1 (de)
CN (1) CN100497632C (de)
AT (1) ATE532870T1 (de)
AU (1) AU7449400A (de)
BR (1) BR0014059B1 (de)
CA (1) CA2391961C (de)
DK (1) DK1219713T3 (de)
ES (1) ES2372806T3 (de)
WO (1) WO2001023591A1 (de)

Families Citing this family (41)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002014518A1 (fr) * 2000-08-17 2002-02-21 Ajinomoto Co., Inc. Procede de modification de transglutaminase de micro-organismes (mtg)
AU2002242986A1 (en) * 2001-03-30 2002-10-21 Ajinomoto Co., Inc. Method for the sectetion and production of protein
US7485438B2 (en) 2002-03-01 2009-02-03 Szu-Yi Chou Method of producing polyvalent antigens
US7101695B2 (en) 2002-03-01 2006-09-05 Szu-Yi Chou Method of producing transglutaminase having broad substrate activity
JP4323147B2 (ja) * 2002-09-10 2009-09-02 天野エンザイム株式会社 トランスグルタミナーゼ生産菌
AU2003268693A1 (en) * 2002-09-30 2004-04-19 Ajinomoto Co., Inc. Method of producing stable isotope-labeled protein
RU2316594C2 (ru) 2003-03-07 2008-02-10 Адзиномото Ко., Инк. Способ продуцирования микробной трансглутаминазы
JP4730302B2 (ja) * 2004-04-20 2011-07-20 味の素株式会社 タンパク質の製造法
EP1919946A1 (de) * 2005-08-18 2008-05-14 Novo Nordisk Health Care AG Transglutaminasevarianten mit verbesserter spezifität
JP2010500886A (ja) * 2006-08-18 2010-01-14 ノボ ノルディスク ヘルス ケア アーゲー 改善された特異性を有するトランスグルタミナーゼ変異体
JPWO2008099898A1 (ja) 2007-02-15 2010-05-27 味の素株式会社 ジスルフィド結合導入トランスグルタミナーゼ
AU2008219238A1 (en) * 2007-02-22 2008-08-28 Novo Nordisk Health Care Ag Transglutaminase variants with improved specificity
ES2480540T3 (es) 2007-03-29 2014-07-28 Ajinomoto Co., Inc. Preparación enzimática para la adherencia y procedimiento de producción de un producto alimenticio moldeado por adherencia
KR20100085083A (ko) 2007-11-19 2010-07-28 아지노모토 가부시키가이샤 섬유 가공물 및 이의 제조법
CN101945995B (zh) * 2008-02-13 2013-06-12 天野酶株式会社 稳定型转谷氨酰胺酶及其制造法
TWI444146B (zh) 2008-09-25 2014-07-11 Ajinomoto Kk 黏著成型食品用酵素製劑及黏著成型食品之製造方法
WO2010101256A1 (ja) 2009-03-06 2010-09-10 味の素株式会社 放線菌由来の耐熱性トランスグルタミナーゼ
US20100316764A1 (en) * 2009-06-10 2010-12-16 Engrain, LLC Flour supplement compositions and methods for preparing wheat flour
EP2772546B1 (de) 2011-10-25 2019-05-15 Ajinomoto Co., Inc. Sekretionsproduktionsverfahren für protein
IN2014CN04039A (de) 2011-11-02 2015-10-23 Ajinomoto Kk
AU2012333515B2 (en) 2011-11-02 2015-07-16 Ajinomoto Co., Inc. Method for secreting and producing proteins
CN102586167B (zh) * 2012-03-01 2013-07-24 华南理工大学 一株重组的枯草芽孢杆菌及由其生产转谷氨酰胺酶的方法
CN103060283B (zh) * 2012-12-28 2014-05-28 天津科技大学 一种抑制枯草芽孢杆菌谷氨酰胺转胺酶活性的多肽及其筛选方法和应用
WO2014126260A1 (ja) 2013-02-18 2014-08-21 味の素株式会社 タンパク質の沈殿による製造方法
CN103146661A (zh) * 2013-03-07 2013-06-12 哈尔滨工业大学 MgCl2胁迫提高茂原链霉菌谷氨酰胺转氨酶产量的方法
RU2013119826A (ru) 2013-04-29 2014-11-10 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО "АГРИ") Коринеформная бактерия и способ получения гетерологичных гибридных белков
EP3287523B1 (de) 2015-04-24 2022-06-29 Ajinomoto Co., Inc. Verfahren zur sekretorischen herstellung eines proteins
US11242545B2 (en) 2016-09-01 2022-02-08 Ningxia Eppen Biotech Co., Ltd Corynebacterium for producing L-lysine by fermentation
CN109937258B (zh) 2016-10-21 2023-06-23 味之素株式会社 蛋白质的分泌产生方法
CN110312807B (zh) 2016-10-21 2023-11-17 味之素株式会社 蛋白质的分泌产生方法
CN118773233A (zh) 2017-03-28 2024-10-15 味之素株式会社 生产rna的方法
WO2019078095A1 (en) 2017-10-16 2019-04-25 Ajinomoto Co., Inc. PROCESS FOR PRODUCTION OF PROTEIN HAVING ALPHA-HYDROXYLANTIC PEPTIDYLGLYCIN MONOOXYGENASE ACTIVITY
CN107574159B (zh) * 2017-10-26 2020-05-08 江南大学 一种以活性形式表达的谷氨酰胺转氨酶的突变体
EP3756466A4 (de) 2018-02-20 2021-11-24 Ajinomoto Co., Inc. Verfahren zur induzierung von rna-silencing
KR102744950B1 (ko) 2018-04-20 2024-12-20 아지노모토 가부시키가이샤 단백질의 분비 생산법
CN113528479B (zh) * 2020-04-13 2023-01-24 中国科学院微生物研究所 一种转谷氨酰胺酶的高效制备方法及其专用工程菌
JPWO2021251383A1 (de) 2020-06-11 2021-12-16
CN111944778B (zh) * 2020-08-14 2022-06-21 安徽医学高等专科学校 谷氨酰胺转胺酶突变体及其编码基因和应用
EP4368722A1 (de) 2021-07-07 2024-05-15 Ajinomoto Co., Inc. Verfahren zur sekretorischen herstellung von nichtnatürlichen aminosäurehaltigen proteinen
EP4509613A1 (de) 2022-04-15 2025-02-19 Ajinomoto Co., Inc. Verfahren zur herstellung von kultiviertem fleisch
EP4526437A1 (de) * 2022-05-20 2025-03-26 Curie Co. Inc. Propeptidvarianten zur modulation der aktivität von transglutaminasen

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR940003653B1 (ko) * 1985-04-05 1994-04-25 상꾜가부시끼가이샤 인체 췌장 엘라스타제의 제조방법
RU2091490C1 (ru) 1985-10-25 1997-09-27 Зимодженетикс, Инк. Способ получения гетерологичного полипептида в эукариотических микроорганизмов
US4965194A (en) * 1986-08-21 1990-10-23 Toyo Boseki Kabushiki Kaisha Pyruvate oxidase and an analytical method using the same
WO1988009821A1 (en) * 1987-06-12 1988-12-15 Massachusetts Institute Of Technology Coryneform expression and secretion system
JPH05244947A (ja) * 1992-03-04 1993-09-24 Mercian Corp 新規ポストプロリンエンドペプチダーゼおよびその製造方法
JPH0736754B2 (ja) 1993-07-16 1995-04-26 三共株式会社 ヒト・膵臓エラスターゼｉｉｉｂ
JPH09316095A (ja) * 1996-05-27 1997-12-09 Mitsubishi Chem Corp 新規シグナルペプチド
JP3711658B2 (ja) * 1996-10-07 2005-11-02 味の素株式会社 コリネバクテリウム・アンモニアゲネス由来の新規な細胞表層蛋白質
JP3305609B2 (ja) 1997-03-06 2002-07-24 ダイハツ工業株式会社 車両用スペアタイヤの支持構造
JPH10244947A (ja) 1997-03-06 1998-09-14 Toyoda Gosei Co Ltd ステアリングホイール芯金

Also Published As

Publication number Publication date
WO2001023591A1 (fr) 2001-04-05
CA2391961C (en) 2008-12-02
US20030082746A1 (en) 2003-05-01
DK1219713T3 (da) 2011-12-12
KR100565030B1 (ko) 2006-03-30
BR0014059A (pt) 2002-05-14
BR0014059B1 (pt) 2013-02-05
ES2372806T3 (es) 2012-01-26
EP1219713A1 (de) 2002-07-03
US7723067B2 (en) 2010-05-25
CN100497632C (zh) 2009-06-10
CA2391961A1 (en) 2001-04-05
EP1219713B1 (de) 2011-11-09
EP1219713A4 (de) 2005-02-02
US7972829B2 (en) 2011-07-05
AU7449400A (en) 2001-04-30
CN1377413A (zh) 2002-10-30
US20100297729A1 (en) 2010-11-25
JP4320769B2 (ja) 2009-08-26
KR20020038787A (ko) 2002-05-23

Similar Documents

Publication Publication Date Title
ATE532870T1 (de) Verfahren zur herstellung von transglutaminase
DE60234247D1 (de) Verfahren zur sekretion und produktion von protein
CS9100241A2 (en) Method of composite proteins' enzymic splitting
ATE372383T1 (de) Verfahren zur herstellung von l-aminosäuren
MY148842A (en) Methods for producing protein partial hydrolysates and infant formulas containing the same
DE502006006800D1 (de) Verfahren zur fermentativen Herstellung von Proteinen
DE502006008613D1 (de) Verfahren zur fermentativen Herstellung von Proteinen
ATE480624T1 (de) Yapsin-mangelmutantenstamm von hansenula polymorpha sowie verfahren zur herstellung rekombinanter proteine damit
ATE329927T1 (de) Rekombinante coryneformbakterie die glyceraldehyde-3-phosphate dehydrogenase -2 überexprimieren , und verfahren zur herstellung von l-lysine
ATE171728T1 (de) Trypsintyp-enzyme, dafür kodierende nukleinsäuresequenz und verfahren zur herstellung des enzymes
DE69110952D1 (de) Penicillin-G-Acylase, dafür kodierendes Gen und Verfahren zur Herstellung von diesem Enzym.
Brennan et al. Signal peptides and signal peptidase in Drosophila melanogaster.
EP0127658B1 (de) Verfahren zur herstellung reifer proteine aus schmelzproteinen hergestellt in pro- oder eukaryotischen zellen
DK0776971T3 (da) Hyperthermostabilt proteasegen
ATE106448T1 (de) Verfahren zur herstellung von hinpi- restriktionsendonuklease und -methylase.
ATE439432T1 (de) Verfahren zur herstellung von säugetier-trypsinen
ATE334203T1 (de) Verfahren zur herstellung von sekundären metaboliten
BR9000445A (pt) Processo para a producao de glucagon,sequencia de dna codificando glucagon,vetor de expressao,transformante e glucagon purificado
ATE277169T1 (de) Verfahren zur herstellung von proteinen durch kontrollierte proteolytische spaltung von pro- proteinen
DE3586410D1 (de) Verfahren zur herstellung von neutraler protease.
DE68913055D1 (de) Verfahren zur Herstellung von AseI-Restriktionsendonuklease und -methylase.
ATE522604T1 (de) Verfahren zur herstellung von proteinen durch elektropulsierung
DE69132845D1 (de) Verfahren zur Herstellung von Proteinen durch Verwendung von CKS-Fusionsproteinen
ATE191007T1 (de) Verfahren zur rekombinanten herstellung von proteinen in der hefe hansenula
ATE269903T1 (de) Verbessertes, induktionsfreies verfahren zur gentechnischen herstellung von glutarylamidase