[go: up one dir, main page]

CY1123882T1 - Επισημασμενα νουκλεοτιδια - Google Patents

Επισημασμενα νουκλεοτιδια

Info

Publication number
CY1123882T1
CY1123882T1 CY20211100153T CY211100153T CY1123882T1 CY 1123882 T1 CY1123882 T1 CY 1123882T1 CY 20211100153 T CY20211100153 T CY 20211100153T CY 211100153 T CY211100153 T CY 211100153T CY 1123882 T1 CY1123882 T1 CY 1123882T1
Authority
CY
Cyprus
Prior art keywords
labeled nucleotides
nucleosides
disclosed
group
binding agents
Prior art date
Application number
CY20211100153T
Other languages
English (en)
Inventor
Shankar Balasubramanian
Colin Barnes
Xiaohai Liu
Harold Swerdlow
Original Assignee
Illumina Cambridge Limited
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=26246831&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CY1123882(T1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority claimed from GBGB0129012.1A external-priority patent/GB0129012D0/en
Application filed by Illumina Cambridge Limited filed Critical Illumina Cambridge Limited
Publication of CY1123882T1 publication Critical patent/CY1123882T1/el

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
    • C07H19/10—Pyrimidine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16—Purine radicals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16—Purine radicals
    • C07H19/20—Purine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/186—Modifications characterised by incorporating a non-extendable or blocking moiety
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
    • C12Q2535/113—Cycle sequencing

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

Δημοσιεύονται νουκλεοσίδια και νουκλεοτίδια που συνδέονται με ανιχνεύσιμες επισημάνσεις μέσω μιας ομάδας παραγόντων σύνδεσης που διαχωρίζονται.
CY20211100153T 2001-12-04 2021-02-23 Επισημασμενα νουκλεοτιδια CY1123882T1 (el)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
GBGB0129012.1A GB0129012D0 (en) 2001-12-04 2001-12-04 Labelled nucleotides
US10/227,131 US7057026B2 (en) 2001-12-04 2002-08-23 Labelled nucleotides
EP02781434.2A EP1451351B1 (en) 2001-12-04 2002-12-04 Labelled nucleotides
EP10180384.9A EP2338893B9 (en) 2001-12-04 2002-12-04 Labelled nucleotides
PCT/GB2002/005474 WO2003048387A2 (en) 2001-12-04 2002-12-04 Labelled nucleotides

Publications (1)

Publication Number Publication Date
CY1123882T1 true CY1123882T1 (el) 2022-05-27

Family

ID=26246831

Family Applications (2)

Application Number Title Priority Date Filing Date
CY20171100360T CY1118765T1 (el) 2001-12-04 2017-03-21 Επισημασμενα νουκλεοτιδια (nucleotides)
CY20211100153T CY1123882T1 (el) 2001-12-04 2021-02-23 Επισημασμενα νουκλεοτιδια

Family Applications Before (1)

Application Number Title Priority Date Filing Date
CY20171100360T CY1118765T1 (el) 2001-12-04 2017-03-21 Επισημασμενα νουκλεοτιδια (nucleotides)

Country Status (16)

Country Link
US (4) US7057026B2 (el)
EP (4) EP2338893B9 (el)
JP (1) JP2005511058A (el)
KR (1) KR20050044668A (el)
CN (1) CN1617937A (el)
AU (1) AU2002349155A1 (el)
BR (1) BR0214683A (el)
CA (1) CA2469066A1 (el)
CY (2) CY1118765T1 (el)
DK (2) DK1451351T3 (el)
ES (2) ES2620131T3 (el)
IL (1) IL162170A0 (el)
IS (1) IS7291A (el)
PT (2) PT1451351T (el)
SI (1) SI3266791T1 (el)
WO (1) WO2003048387A2 (el)

Families Citing this family (920)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7875440B2 (en) 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6780591B2 (en) 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6818395B1 (en) 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
EP1337541B1 (en) 2000-10-06 2007-03-07 The Trustees of Columbia University in the City of New York Massive parallel method for decoding DNA and RNA
US9708358B2 (en) 2000-10-06 2017-07-18 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding DNA and RNA
US7297518B2 (en) 2001-03-12 2007-11-20 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences by asynchronous base extension
US7057026B2 (en) * 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
GB0129012D0 (en) 2001-12-04 2002-01-23 Solexa Ltd Labelled nucleotides
US20030215816A1 (en) * 2002-05-20 2003-11-20 Narayan Sundararajan Method for sequencing nucleic acids by observing the uptake of nucleotides modified with bulky groups
WO2004018497A2 (en) 2002-08-23 2004-03-04 Solexa Limited Modified nucleotides for polynucleotide sequencing
AU2003259352A1 (en) 2002-08-23 2004-03-11 Solexa Limited Labelled nucleotides
US11008359B2 (en) 2002-08-23 2021-05-18 Illumina Cambridge Limited Labelled nucleotides
US7414116B2 (en) 2002-08-23 2008-08-19 Illumina Cambridge Limited Labelled nucleotides
US20050032081A1 (en) * 2002-12-13 2005-02-10 Jingyue Ju Biomolecular coupling methods using 1,3-dipolar cycloaddition chemistry
US20100022414A1 (en) 2008-07-18 2010-01-28 Raindance Technologies, Inc. Droplet Libraries
US20060078893A1 (en) 2004-10-12 2006-04-13 Medical Research Council Compartmentalised combinatorial chemistry by microfluidic control
GB0307428D0 (en) 2003-03-31 2003-05-07 Medical Res Council Compartmentalised combinatorial chemistry
GB0307403D0 (en) 2003-03-31 2003-05-07 Medical Res Council Selection by compartmentalised screening
US7572581B2 (en) * 2003-06-30 2009-08-11 Roche Molecular Systems, Inc. 2′-terminator nucleotide-related methods and systems
WO2005044836A2 (de) 2003-11-05 2005-05-19 Genovoxx Gmbh Makromolekulare nukleotidverbindungen und methoden zu deren anwendung
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
ATE463584T1 (de) 2004-02-19 2010-04-15 Helicos Biosciences Corp Verfahren zur analyse von polynukleotidsequenzen
US7622279B2 (en) * 2004-03-03 2009-11-24 The Trustees Of Columbia University In The City Of New York Photocleavable fluorescent nucleotides for DNA sequencing on chip constructed by site-specific coupling chemistry
US20050221339A1 (en) 2004-03-31 2005-10-06 Medical Research Council Harvard University Compartmentalised screening by microfluidic control
WO2006073436A2 (en) * 2004-04-29 2006-07-13 The Trustees Of Columbia University In The City Of New York Mass tag pcr for multiplex diagnostics
WO2006007207A2 (en) 2004-05-25 2006-01-19 Helicos Biosciences Corporation Methods and devices for nucleic acid sequence determination
US7476734B2 (en) * 2005-12-06 2009-01-13 Helicos Biosciences Corporation Nucleotide analogs
US7968287B2 (en) 2004-10-08 2011-06-28 Medical Research Council Harvard University In vitro evolution in microfluidic systems
EP1828412B2 (en) * 2004-12-13 2019-01-09 Illumina Cambridge Limited Improved method of nucleotide detection
GB0427236D0 (en) 2004-12-13 2005-01-12 Solexa Ltd Improved method of nucleotide detection
US7220549B2 (en) 2004-12-30 2007-05-22 Helicos Biosciences Corporation Stabilizing a nucleic acid for nucleic acid sequencing
EP1879906A4 (en) * 2005-01-05 2009-07-22 Advanced Genetic Analysis Corp REVERSIBLE NUCLEOTIDE TERMINATORS AND USES THEREOF
US7482120B2 (en) 2005-01-28 2009-01-27 Helicos Biosciences Corporation Methods and compositions for improving fidelity in a nucleic acid synthesis reaction
EP2230316A1 (en) * 2005-02-01 2010-09-22 AB Advanced Genetic Analysis Corporation Nucleic acid sequencing by performing successive cycles of duplex extension
EP2233583A1 (en) 2005-02-01 2010-09-29 AB Advanced Genetic Analysis Corporation Nucleic acid sequencing by performing successive cycles of duplex extension
GB0507835D0 (en) * 2005-04-18 2005-05-25 Solexa Ltd Method and device for nucleic acid sequencing using a planar wave guide
WO2006120433A1 (en) 2005-05-10 2006-11-16 Solexa Limited Improved polymerases
GB0509508D0 (en) * 2005-05-10 2005-06-15 Solexa Ltd Improved polymerases
US20060281100A1 (en) * 2005-06-14 2006-12-14 Shen Gene G Thiotriphosphate nucleotide dye terminators
WO2007002204A2 (en) * 2005-06-21 2007-01-04 The Trustees Of Columbia University In The City Of New York Pyrosequencing methods and related compostions
GB0517097D0 (en) 2005-08-19 2005-09-28 Solexa Ltd Modified nucleosides and nucleotides and uses thereof
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
WO2007053702A2 (en) 2005-10-31 2007-05-10 The Trustees Of Columbia University In The City Of New York Synthesis of four color 3'-o-allyl modified photocleavable fluorescent nucleotides and related methods
US8796432B2 (en) 2005-10-31 2014-08-05 The Trustees Of Columbia University In The City Of New York Chemically cleavable 3'-o-allyl-DNTP-allyl-fluorophore fluorescent nucleotide analogues and related methods
AU2006318462A1 (en) * 2005-11-21 2007-05-31 The Trustees Of Columbia University In The City Of New York Multiplex digital immuno-sensing using a library of photocleavable mass tags
EP3913375A1 (en) 2006-01-11 2021-11-24 Bio-Rad Laboratories, Inc. Microfluidic devices and methods of use in the formation and control of nanoreactors
RU2487169C2 (ru) * 2006-01-19 2013-07-10 Зе Рисерч Фаундейшн Оф Стейт Юниверсити Оф Нью Йорк Способы и приборы для обнаружения и идентификации закодированных гранул и биологических молекул
US7397546B2 (en) 2006-03-08 2008-07-08 Helicos Biosciences Corporation Systems and methods for reducing detected intensity non-uniformity in a laser beam
CN101460953B (zh) * 2006-03-31 2012-05-30 索雷克萨公司 用于合成分析的序列的系统和装置
ES2829295T3 (es) 2006-04-04 2021-05-31 Keygene Nv Detección de alto rendimiento de marcadores moleculares basados en AFLP y secuenciación de alto rendimiento
CN101495654A (zh) * 2006-04-19 2009-07-29 阿普里拉股份有限公司 无凝胶珠基测序的试剂、方法和文库
US9562837B2 (en) 2006-05-11 2017-02-07 Raindance Technologies, Inc. Systems for handling microfludic droplets
EP3031918B1 (en) 2006-05-11 2018-03-14 Raindance Technologies Inc. Microfluidic devices
US8178360B2 (en) 2006-05-18 2012-05-15 Illumina Cambridge Limited Dye compounds and the use of their labelled conjugates
WO2007146158A1 (en) * 2006-06-07 2007-12-21 The Trustees Of Columbia University In The City Of New York Dna sequencing by nanopore using modified nucleotides
EP2044217A2 (en) * 2006-06-30 2009-04-08 Applera Corporation Reversible terminator nucleotides and methods of use
US8178300B2 (en) 2006-07-12 2012-05-15 Keygene N.V. Method for the identification of the clonal source of a restriction fragment
WO2008021123A1 (en) 2006-08-07 2008-02-21 President And Fellows Of Harvard College Fluorocarbon emulsion stabilizing surfactants
JP5244103B2 (ja) 2006-08-09 2013-07-24 ホームステッド クリニカル コーポレイション 器官特異的蛋白質およびその使用方法
WO2008027558A2 (en) 2006-08-31 2008-03-06 Codon Devices, Inc. Iterative nucleic acid assembly using activation of vector-encoded traits
US8399188B2 (en) 2006-09-28 2013-03-19 Illumina, Inc. Compositions and methods for nucleotide sequencing
EP2076609A1 (en) 2006-10-10 2009-07-08 Illumina Inc. Compositions and methods for representational selection of nucleic acids fro complex mixtures using hybridization
US20080242560A1 (en) * 2006-11-21 2008-10-02 Gunderson Kevin L Methods for generating amplified nucleic acid arrays
US7883869B2 (en) 2006-12-01 2011-02-08 The Trustees Of Columbia University In The City Of New York Four-color DNA sequencing by synthesis using cleavable fluorescent nucleotide reversible terminators
US7897737B2 (en) 2006-12-05 2011-03-01 Lasergen, Inc. 3′-OH unblocked, nucleotides and nucleosides, base modified with photocleavable, terminating groups and methods for their use in DNA sequencing
MX2009005936A (es) * 2006-12-05 2010-01-15 Lasergen Inc Nucleotidos y nucleosidos marcados de foto-escision y nucleotidos y nucleosidos marcados y metodos para su uso en secunciacion de adn.
WO2008093098A2 (en) 2007-02-02 2008-08-07 Illumina Cambridge Limited Methods for indexing samples and sequencing multiple nucleotide templates
US11035823B2 (en) 2009-03-17 2021-06-15 Qiagen Sciences, Llc Methods and devices for sequencing nucleic acids in smaller batches
US11940413B2 (en) 2007-02-05 2024-03-26 IsoPlexis Corporation Methods and devices for sequencing nucleic acids in smaller batches
US8612161B2 (en) 2008-03-19 2013-12-17 Intelligent Biosystems Inc. Methods and compositions for base calling nucleic acids
US8481259B2 (en) 2007-02-05 2013-07-09 Intelligent Bio-Systems, Inc. Methods and devices for sequencing nucleic acids in smaller batches
WO2008097559A2 (en) 2007-02-06 2008-08-14 Brandeis University Manipulation of fluids and reactions in microfluidic systems
CN101631796B (zh) * 2007-03-09 2013-08-07 独立行政法人理化学研究所 具有由单核苷或单核苷酸衍生的结构的化合物、核酸、标记物以及核酸检测方法和试剂盒
JP5750209B2 (ja) * 2007-03-16 2015-07-15 アプタ バイオサイエンス リミテッド 機能性分子、機能性分子合成用アミダイド、及び標的物質解析方法
US8592221B2 (en) 2007-04-19 2013-11-26 Brandeis University Manipulation of fluids, fluid components and reactions in microfluidic systems
US7678894B2 (en) * 2007-05-18 2010-03-16 Helicos Biosciences Corporation Nucleotide analogs
US20090099027A1 (en) * 2007-08-23 2009-04-16 Applied Biosystems Inc. Methods of Modifying Support Surfaces for the Immobilization of Particles and the Use of the Immobilized Particles for Analyzing Nucleic Acids
WO2009029728A1 (en) * 2007-08-29 2009-03-05 Applied Biosystems Inc. Alternative nucleic acid sequencing methods
EP2725107B1 (en) 2007-10-19 2018-08-29 The Trustees of Columbia University in the City of New York DNA sequencing with non-fluorescent nucleotide reversible terminators and cleavable label modified ddNTPs and nucleic acid comprising inosine with reversible terminators
EP2207900B1 (en) 2007-10-19 2015-04-29 The Trustees of Columbia University in the City of New York Design and synthesis of cleavable fluorescent nucleotides as reversible terminators for dna sequencing by synthesis
US7811810B2 (en) 2007-10-25 2010-10-12 Industrial Technology Research Institute Bioassay system including optical detection apparatuses, and method for detecting biomolecules
US7767441B2 (en) * 2007-10-25 2010-08-03 Industrial Technology Research Institute Bioassay system including optical detection apparatuses, and method for detecting biomolecules
WO2009067628A1 (en) 2007-11-20 2009-05-28 Applied Biosystems Inc. Reversible di-nucleotide terminator sequencing
US8652781B2 (en) 2008-02-12 2014-02-18 Pacific Biosciences Of California, Inc. Cognate sampling kinetics
CN102027136A (zh) 2008-03-17 2011-04-20 表现研究有限公司 发现表达连接基因
US9017973B2 (en) 2008-03-19 2015-04-28 Intelligent Biosystems, Inc. Methods and compositions for incorporating nucleotides
WO2009145820A2 (en) * 2008-03-31 2009-12-03 Pacific Biosciences Of California, Inc. Generation of modified polymerases for improved accuracy in single molecule sequencing
US8420366B2 (en) * 2008-03-31 2013-04-16 Pacific Biosciences Of California, Inc. Generation of modified polymerases for improved accuracy in single molecule sequencing
US8133672B2 (en) * 2008-03-31 2012-03-13 Pacific Biosciences Of California, Inc. Two slow-step polymerase enzyme systems and methods
US8999676B2 (en) 2008-03-31 2015-04-07 Pacific Biosciences Of California, Inc. Recombinant polymerases for improved single molecule sequencing
US8039817B2 (en) 2008-05-05 2011-10-18 Illumina, Inc. Compensator for multiple surface imaging
WO2009137521A2 (en) * 2008-05-07 2009-11-12 Illumina, Inc. Compositions and methods for providing substances to and from an array
WO2009152353A2 (en) 2008-06-11 2009-12-17 Lasergen, Inc. Nucleotides and nucleosides and methods for their use in dna sequencing
WO2010003132A1 (en) 2008-07-02 2010-01-07 Illumina Cambridge Ltd. Using populations of beads for the fabrication of arrays on surfaces
WO2010003153A2 (en) * 2008-07-03 2010-01-07 Life Technologies Corporation Methylation analysis of mate pairs
US12038438B2 (en) 2008-07-18 2024-07-16 Bio-Rad Laboratories, Inc. Enzyme quantification
US8173198B2 (en) * 2008-07-23 2012-05-08 Life Technologies Corporation Deposition of metal oxides onto surfaces as an immobilization vehicle for carboxylated or phophated particles or polymers
US10759824B2 (en) 2008-09-03 2020-09-01 Quantumdx Group Limited Design, synthesis and use of synthetic nucleotides comprising charge mass tags
US8871921B2 (en) * 2008-09-03 2014-10-28 Quantumdx Group Ltd. Design, synthesis and use of synthetic nucleotides comprising charge mass tags
EP2321429B1 (en) * 2008-09-03 2016-08-17 Quantumdx Group Limited Methods and kits for nucleic acid sequencing
EP2326733A2 (en) * 2008-09-05 2011-06-01 Pacific Biosciences of California, Inc. Engineering polymerases and reaction conditions for modified incorporation properties
US8383345B2 (en) 2008-09-12 2013-02-26 University Of Washington Sequence tag directed subassembly of short sequencing reads into long sequencing reads
US8728764B2 (en) 2008-10-02 2014-05-20 Illumina Cambridge Limited Nucleic acid sample enrichment for sequencing applications
WO2010048337A2 (en) 2008-10-22 2010-04-29 Illumina, Inc. Preservation of information related to genomic dna methylation
AU2009319907B2 (en) 2008-11-03 2015-10-01 The Regents Of The University Of California Methods for detecting modification resistant nucleic acids
WO2010059206A2 (en) 2008-11-19 2010-05-27 Pacific Biosciences Of California, Inc. Modular nucleotide compositions and uses therefor
WO2010068702A2 (en) * 2008-12-10 2010-06-17 Illumina, Inc. Methods and compositions for hybridizing nucleic acids
WO2010075188A2 (en) 2008-12-23 2010-07-01 Illumina Inc. Multibase delivery for long reads in sequencing by synthesis protocols
EP2379751B1 (en) 2009-01-13 2013-03-20 Keygene N.V. Novel genome sequencing strategies
WO2010111231A1 (en) 2009-03-23 2010-09-30 Raindance Technologies, Inc. Manipulation of microfluidic droplets
WO2010111690A2 (en) 2009-03-27 2010-09-30 Life Technologies Corporation Polymerase compositions and methods
GB0907372D0 (en) 2009-04-29 2009-06-10 Invitrogen Dynal As Particles
US12129514B2 (en) 2009-04-30 2024-10-29 Molecular Loop Biosolutions, Llc Methods and compositions for evaluating genetic markers
CA2760439A1 (en) 2009-04-30 2010-11-04 Good Start Genetics, Inc. Methods and compositions for evaluating genetic markers
WO2010127304A2 (en) * 2009-05-01 2010-11-04 Illumina, Inc. Sequencing methods
US8632975B2 (en) 2009-06-05 2014-01-21 Life Technologies Corporation Nucleotide transient binding for sequencing methods
EP2456892B1 (en) 2009-07-24 2014-10-01 Illumina, Inc. Method for sequencing a polynucleotide template
ES2763386T3 (es) 2009-08-25 2020-05-28 Illumina Inc Métodos para seleccionar y amplificar polinucleótidos
EP2486409A1 (en) 2009-10-09 2012-08-15 Universite De Strasbourg Labelled silica-based nanomaterial with enhanced properties and uses thereof
US9315860B2 (en) 2009-10-26 2016-04-19 Genovoxx Gmbh Conjugates of nucleotides and method for the application thereof
US10837883B2 (en) 2009-12-23 2020-11-17 Bio-Rad Laboratories, Inc. Microfluidic systems and methods for reducing the exchange of molecules between droplets
EP2531880B1 (en) 2010-02-01 2016-08-03 Illumina Inc. Focusing methods and optical systems and assemblies using the same
US9678055B2 (en) 2010-02-08 2017-06-13 Genia Technologies, Inc. Methods for forming a nanopore in a lipid bilayer
US8324914B2 (en) 2010-02-08 2012-12-04 Genia Technologies, Inc. Systems and methods for characterizing a molecule
US20110192723A1 (en) * 2010-02-08 2011-08-11 Genia Technologies, Inc. Systems and methods for manipulating a molecule in a nanopore
US9605307B2 (en) 2010-02-08 2017-03-28 Genia Technologies, Inc. Systems and methods for forming a nanopore in a lipid bilayer
US10351905B2 (en) 2010-02-12 2019-07-16 Bio-Rad Laboratories, Inc. Digital analyte analysis
US9399797B2 (en) 2010-02-12 2016-07-26 Raindance Technologies, Inc. Digital analyte analysis
EP4484577A3 (en) 2010-02-12 2025-03-26 Bio-Rad Laboratories, Inc. Digital analyte analysis
US9366632B2 (en) 2010-02-12 2016-06-14 Raindance Technologies, Inc. Digital analyte analysis
WO2011106368A2 (en) 2010-02-23 2011-09-01 Illumina, Inc. Amplification methods to minimise sequence specific bias
DE202011003570U1 (de) 2010-03-06 2012-01-30 Illumina, Inc. Systeme und Vorrichtungen zum Detektieren optischer Signale aus einer Probe
US8951940B2 (en) 2010-04-01 2015-02-10 Illumina, Inc. Solid-phase clonal amplification and related methods
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
ES2555106T3 (es) 2010-04-05 2015-12-29 Prognosys Biosciences, Inc. Ensayos biológicos codificados espacialmente
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
WO2011159942A1 (en) 2010-06-18 2011-12-22 Illumina, Inc. Conformational probes and methods for sequencing nucleic acids
US8986930B2 (en) 2010-07-12 2015-03-24 Pacific Biosciences Of California, Inc. Sequencing reactions with alkali metal cations for pulse width control
US9029103B2 (en) 2010-08-27 2015-05-12 Illumina Cambridge Limited Methods for sequencing polynucleotides
WO2012034007A2 (en) 2010-09-10 2012-03-15 Bio-Rad Laboratories, Inc. Size selection of dna for chromatin analysis
US8483969B2 (en) 2010-09-17 2013-07-09 Illuminia, Inc. Variation analysis for multiple templates on a solid support
US8759038B2 (en) 2010-09-29 2014-06-24 Illumina Cambridge Limited Compositions and methods for sequencing nucleic acids
EP3447155A1 (en) 2010-09-30 2019-02-27 Raindance Technologies, Inc. Sandwich assays in droplets
WO2012055929A1 (en) 2010-10-26 2012-05-03 Illumina, Inc. Sequencing methods
EP2632593B1 (en) 2010-10-27 2021-09-29 Illumina, Inc. Flow cells for biological or chemical analysis
US8575071B2 (en) 2010-11-03 2013-11-05 Illumina, Inc. Reducing adapter dimer formation
US9074251B2 (en) 2011-02-10 2015-07-07 Illumina, Inc. Linking sequence reads using paired code tags
AU2011323107B2 (en) 2010-11-05 2015-09-10 Illumina, Inc. Linking sequence reads using paired code tags
WO2012064975A1 (en) 2010-11-12 2012-05-18 Gen9, Inc. Protein arrays and methods of using and making the same
AU2011338841B2 (en) 2010-11-12 2017-02-16 Gen9, Inc. Methods and devices for nucleic acids synthesis
WO2012074855A2 (en) 2010-11-22 2012-06-07 The Regents Of The University Of California Methods of identifying a cellular nascent rna transcript
WO2013082164A1 (en) 2011-11-28 2013-06-06 Life Technologies Corporation Enhanced ligation reactions
US8845880B2 (en) 2010-12-22 2014-09-30 Genia Technologies, Inc. Nanopore-based single DNA molecule characterization, identification and isolation using speed bumps
US9163281B2 (en) 2010-12-23 2015-10-20 Good Start Genetics, Inc. Methods for maintaining the integrity and identification of a nucleic acid template in a multiplex sequencing reaction
US8951781B2 (en) 2011-01-10 2015-02-10 Illumina, Inc. Systems, methods, and apparatuses to image a sample for biological or chemical analysis
US8883764B1 (en) 2011-01-19 2014-11-11 Illumina, Inc. Formylpyrrole-based heterocycles for nucleic acid attachment to supports
US9581563B2 (en) 2011-01-24 2017-02-28 Genia Technologies, Inc. System for communicating information from an array of sensors
US9110478B2 (en) 2011-01-27 2015-08-18 Genia Technologies, Inc. Temperature regulation of measurement arrays
CA2825815C (en) 2011-01-31 2017-09-05 Illumina, Inc. Methods for reducing nucleic acid damage
AU2012212148B8 (en) 2011-02-02 2017-07-06 University Of Washington Through Its Center For Commercialization Massively parallel contiguity mapping
EP2673614B1 (en) 2011-02-11 2018-08-01 Raindance Technologies, Inc. Method for forming mixed droplets
US9150852B2 (en) 2011-02-18 2015-10-06 Raindance Technologies, Inc. Compositions and methods for molecular labeling
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
US9994894B2 (en) 2011-04-27 2018-06-12 Agct Gmbh Method and components for detecting nucleic acid chains
US20150086981A1 (en) 2011-05-04 2015-03-26 Genovoxx Gmbh Nucleoside-triphosphate conjugate and methods for the use thereof
WO2012162429A2 (en) 2011-05-23 2012-11-29 The Trustees Of Columbia University In The City Of New York Dna sequencing by synthesis using raman and infrared spectroscopy detection
WO2012167142A2 (en) 2011-06-02 2012-12-06 Raindance Technolgies, Inc. Enzyme quantification
US8841071B2 (en) 2011-06-02 2014-09-23 Raindance Technologies, Inc. Sample multiplexing
EP2718465B1 (en) 2011-06-09 2022-04-13 Illumina, Inc. Method of making an analyte array
US9777322B2 (en) 2011-07-08 2017-10-03 Keygene N.V. Sequence based genotyping based on oligonucleotide ligation assays
US8658430B2 (en) 2011-07-20 2014-02-25 Raindance Technologies, Inc. Manipulating droplet size
US20130059296A1 (en) 2011-08-26 2013-03-07 Gen9, Inc. Compositions and Methods For High Fidelity Assembly of Nucleic Acids
WO2013040257A1 (en) 2011-09-13 2013-03-21 Lasergen, Inc. 5-methoxy. 3'-oh unblocked, fast photocleavable terminating nucleotides and methods for nucleic acid sequencing
WO2013049135A1 (en) 2011-09-26 2013-04-04 Gen-Probe Incorporated Algorithms for sequence determinations
US10378051B2 (en) 2011-09-29 2019-08-13 Illumina Cambridge Limited Continuous extension and deblocking in reactions for nucleic acids synthesis and sequencing
EP2768983A4 (en) 2011-10-17 2015-06-03 Good Start Genetics Inc METHODS OF IDENTIFYING MUTATIONS ASSOCIATED WITH DISEASES
AU2012328662B2 (en) 2011-10-28 2015-12-17 Illumina, Inc. Microarray fabrication system and method
WO2013070627A2 (en) 2011-11-07 2013-05-16 Illumina, Inc. Integrated sequencing apparatuses and methods of use
WO2013085710A2 (en) 2011-12-09 2013-06-13 Illumina, Inc. Expanded radix for polymeric tags
WO2013096692A1 (en) 2011-12-21 2013-06-27 Illumina, Inc. Apparatus and methods for kinetic analysis and determination of nucleic acid sequences
WO2013117595A2 (en) 2012-02-07 2013-08-15 Illumina Cambridge Limited Targeted enrichment and amplification of nucleic acids on a support
US9176031B2 (en) 2012-02-24 2015-11-03 Raindance Technologies, Inc. Labeling and sample preparation for sequencing
US8986629B2 (en) 2012-02-27 2015-03-24 Genia Technologies, Inc. Sensor circuit for controlling, detecting, and measuring a molecular complex
NO2694769T3 (el) 2012-03-06 2018-03-03
US9150853B2 (en) 2012-03-21 2015-10-06 Gen9, Inc. Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis
US20130261984A1 (en) 2012-03-30 2013-10-03 Illumina, Inc. Methods and systems for determining fetal chromosomal abnormalities
JPWO2013150902A1 (ja) * 2012-04-02 2015-12-17 国立研究開発法人理化学研究所 非天然型塩基を含むオリゴデオキシヌクレオチドの切断による５’末端リン酸化オリゴデオキシヌクレオチドの製造方法
ES2949570T3 (es) 2012-04-03 2023-09-29 Illumina Inc Cabezal integrado de lectura optoelectrónica y cartucho de fluidos útiles para la secuenciación de ácidos nucleicos
US8209130B1 (en) 2012-04-04 2012-06-26 Good Start Genetics, Inc. Sequence assembly
US8812422B2 (en) 2012-04-09 2014-08-19 Good Start Genetics, Inc. Variant database
US20130274148A1 (en) 2012-04-11 2013-10-17 Illumina, Inc. Portable genetic detection and analysis system and method
US9444880B2 (en) 2012-04-11 2016-09-13 Illumina, Inc. Cloud computing environment for biological data
US10227635B2 (en) 2012-04-16 2019-03-12 Molecular Loop Biosolutions, Llc Capture reactions
AU2013251701A1 (en) 2012-04-24 2014-10-30 Gen9, Inc. Methods for sorting nucleic acids and multiplexed preparative in vitro cloning
US9012022B2 (en) 2012-06-08 2015-04-21 Illumina, Inc. Polymer coatings
WO2013185137A1 (en) 2012-06-08 2013-12-12 Pacific Biosciences Of California, Inc. Modified base detection with nanopore sequencing
WO2013188841A1 (en) 2012-06-15 2013-12-19 Genia Technologies, Inc. Chip set-up and high-accuracy nucleic acid sequencing
US8895249B2 (en) 2012-06-15 2014-11-25 Illumina, Inc. Kinetic exclusion amplification of nucleic acid libraries
CN104685116A (zh) * 2012-06-25 2015-06-03 Gen9股份有限公司 用于核酸组装和高通量测序的方法
CA2878291A1 (en) 2012-07-03 2014-01-09 Sloan Kettering Institute For Cancer Research Quantitative assessment of human t-cell repertoire recovery after allogeneic hematopoietic stem cell transplantation
US9092401B2 (en) 2012-10-31 2015-07-28 Counsyl, Inc. System and methods for detecting genetic variation
EP3243937A1 (en) 2012-07-17 2017-11-15 Counsyl, Inc. System and methods for detecting genetic variation
US9422602B2 (en) 2012-08-15 2016-08-23 Bio-Rad Laboratories, Inc. Methods and compositions for determining nucleic acid degradation
NL2017959B1 (en) 2016-12-08 2018-06-19 Illumina Inc Cartridge assembly
EP3699577B1 (en) 2012-08-20 2023-11-08 Illumina, Inc. System for fluorescence lifetime based sequencing
US9150896B2 (en) 2012-09-06 2015-10-06 Illumina, Inc. Nucleotides and primers with removable blocking groups
NZ705606A (en) 2012-09-19 2018-05-25 Abbvie Biotherapeutics Inc Methods for identifying antibodies with reduced immunogenicity
US9399766B2 (en) 2012-10-01 2016-07-26 Pacific Biosciences Of California, Inc. Recombinant polymerases for incorporation of protein shield nucleotide analogs
WO2014060483A1 (en) 2012-10-17 2014-04-24 Spatial Transcriptomics Ab Methods and product for optimising localised or spatial detection of gene expression in a tissue sample
US9181583B2 (en) 2012-10-23 2015-11-10 Illumina, Inc. HLA typing using selective amplification and sequencing
US9116139B2 (en) 2012-11-05 2015-08-25 Illumina, Inc. Sequence scheduling and sample distribution techniques
US9605309B2 (en) 2012-11-09 2017-03-28 Genia Technologies, Inc. Nucleic acid sequencing using tags
US10829816B2 (en) 2012-11-19 2020-11-10 Apton Biosystems, Inc. Methods of analyte detection
HUE058723T2 (hu) 2012-11-19 2022-09-28 Apton Biosystems Inc Molekuláris analitok digitális elemzése egyetlen molekula kimutatásával
US9683230B2 (en) 2013-01-09 2017-06-20 Illumina Cambridge Limited Sample preparation on a solid support
CN103087131B (zh) * 2013-01-15 2015-07-08 上海交通大学 可逆终端及其合成和在dna合成测序中的用途
US9805407B2 (en) 2013-01-25 2017-10-31 Illumina, Inc. Methods and systems for using a cloud computing environment to configure and sell a biological sample preparation cartridge and share related data
US9759711B2 (en) 2013-02-05 2017-09-12 Genia Technologies, Inc. Nanopore arrays
US9512422B2 (en) 2013-02-26 2016-12-06 Illumina, Inc. Gel patterned surfaces
US9650406B2 (en) 2013-02-28 2017-05-16 Centrillion Technology Holdings Corporation Reversible terminator molecules and methods of their use
US20140249037A1 (en) 2013-03-04 2014-09-04 Fry Laboratories, LLC Method and kit for characterizing microorganisms
WO2014135669A1 (en) 2013-03-08 2014-09-12 Roche Diagnostics Gmbh Egfr mutation blood testing
EP2969479B1 (en) 2013-03-13 2021-05-05 Illumina, Inc. Multilayer fluidic devices and methods for their fabrication
DK2970951T3 (da) 2013-03-13 2019-05-13 Illumina Inc Fremgangsmåder til nukleinsyresekventering
US9146248B2 (en) 2013-03-14 2015-09-29 Intelligent Bio-Systems, Inc. Apparatus and methods for purging flow cells in nucleic acid sequencing instruments
EP2971159B1 (en) 2013-03-14 2019-05-08 Molecular Loop Biosolutions, LLC Methods for analyzing nucleic acids
US10421996B2 (en) 2013-03-14 2019-09-24 Illumina, Inc. Modified polymerases for improved incorporation of nucleotide analogues
US9591268B2 (en) 2013-03-15 2017-03-07 Qiagen Waltham, Inc. Flow cell alignment methods and systems
US10648026B2 (en) 2013-03-15 2020-05-12 The Trustees Of Columbia University In The City Of New York Raman cluster tagged molecules for biological imaging
US10767221B2 (en) 2013-03-15 2020-09-08 Illumina, Inc. Enzyme-linked nucleotides
US9193998B2 (en) 2013-03-15 2015-11-24 Illumina, Inc. Super resolution imaging
US9255265B2 (en) 2013-03-15 2016-02-09 Illumina, Inc. Methods for producing stranded cDNA libraries
JP6333297B2 (ja) 2013-03-15 2018-05-30 イルミナ ケンブリッジ リミテッド 修飾ヌクレオシドまたは修飾ヌクレオチド
US20140274747A1 (en) 2013-03-15 2014-09-18 Illumina, Inc. Super resolution imaging
US8847799B1 (en) 2013-06-03 2014-09-30 Good Start Genetics, Inc. Methods and systems for storing sequence read data
CN105849275B (zh) 2013-06-25 2020-03-17 普罗格诺西斯生物科学公司 检测样品中生物靶标的空间分布的方法和系统
KR102177225B1 (ko) 2013-07-01 2020-11-10 일루미나, 인코포레이티드 촉매-무함유 표면 작용화 및 중합체 그라프팅
WO2015002789A1 (en) 2013-07-03 2015-01-08 Illumina, Inc. Sequencing by orthogonal synthesis
CA3091557C (en) 2013-08-08 2022-10-18 Illumina, Inc. Fluidic system for reagent delivery to a flow cell
WO2015027112A1 (en) 2013-08-22 2015-02-26 Apton Biosystems, Inc. Digital analysis of molecular analytes using electrical methods
WO2015031849A1 (en) 2013-08-30 2015-03-05 Illumina, Inc. Manipulation of droplets on hydrophilic or variegated-hydrophilic surfaces
CN103484106B (zh) * 2013-09-05 2015-08-19 上海交通大学 四色荧光标记可逆终端及其在dna测序中的用途
US9352315B2 (en) 2013-09-27 2016-05-31 Taiwan Semiconductor Manufacturing Company, Ltd. Method to produce chemical pattern in micro-fluidic structure
US10410739B2 (en) 2013-10-04 2019-09-10 Life Technologies Corporation Methods and systems for modeling phasing effects in sequencing using termination chemistry
US11901041B2 (en) 2013-10-04 2024-02-13 Bio-Rad Laboratories, Inc. Digital analysis of nucleic acid modification
US9551697B2 (en) 2013-10-17 2017-01-24 Genia Technologies, Inc. Non-faradaic, capacitively coupled measurement in a nanopore cell array
EP3058096A1 (en) 2013-10-18 2016-08-24 Good Start Genetics, Inc. Methods for assessing a genomic region of a subject
US10851414B2 (en) 2013-10-18 2020-12-01 Good Start Genetics, Inc. Methods for determining carrier status
EP3060918B1 (en) 2013-10-23 2019-09-18 Genia Technologies, Inc. High speed molecular sensing with nanopores
US9567630B2 (en) 2013-10-23 2017-02-14 Genia Technologies, Inc. Methods for forming lipid bilayers on biochips
US10540783B2 (en) 2013-11-01 2020-01-21 Illumina, Inc. Image analysis useful for patterned objects
MX392471B (es) 2013-11-17 2025-03-24 Quantum Si Inc Sistema optico y chip de ensayo para sondear, detectar y analizar moleculas
EP2876166B1 (en) * 2013-11-20 2016-12-14 Roche Diagnostics GmbH New compound for sequencing by synthesis
PT3077943T (pt) 2013-12-03 2020-08-21 Illumina Inc Métodos e sistemas para analisar dados de imagem
ES2971473T3 (es) 2013-12-10 2024-06-05 Illumina Inc Biosensores para análisis biológicos o químicos y métodos para la fabricación de los mismos
US9944977B2 (en) 2013-12-12 2018-04-17 Raindance Technologies, Inc. Distinguishing rare variations in a nucleic acid sequence from a sample
WO2015095226A2 (en) 2013-12-20 2015-06-25 Illumina, Inc. Preserving genomic connectivity information in fragmented genomic dna samples
KR102475314B1 (ko) 2013-12-23 2022-12-06 일루미나, 인코포레이티드 광 방출의 검출을 개선시키기 위한 구조화 기판 및 이와 관련한 방법
WO2015103367A1 (en) 2013-12-31 2015-07-09 Raindance Technologies, Inc. System and method for detection of rna species
US10537889B2 (en) 2013-12-31 2020-01-21 Illumina, Inc. Addressable flow cell using patterned electrodes
WO2015108663A1 (en) 2014-01-16 2015-07-23 Illumina, Inc. Amplicon preparation and sequencing on solid supports
US9677132B2 (en) 2014-01-16 2017-06-13 Illumina, Inc. Polynucleotide modification on solid support
CA2939642C (en) 2014-02-13 2022-05-31 Illumina, Inc. Integrated consumer genomic services
BR122022007092B8 (pt) 2014-02-18 2023-01-31 Illumina Inc Método para construir um perfil de dna, método para construir uma biblioteca de ácido nucléico, biblioteca de ácido nucléico, pluralidade de iniciadores e kit
EP3698874B1 (en) 2014-03-11 2024-07-31 Illumina, Inc. Disposable, integrated microfluidic cartridge and methods of making the same
FR3020071B1 (fr) 2014-04-17 2017-12-22 Dna Script Procede de synthese d'acides nucleiques, notamment d'acides nucleiques de grande longueur, utilisation du procede et kit pour la mise en œuvre du procede
JP6412954B2 (ja) 2014-04-29 2018-10-24 イルミナ インコーポレイテッド 鋳型切換え及びタグメンテーションを用いる単一細胞の遺伝子発現の多重分析
WO2015175530A1 (en) 2014-05-12 2015-11-19 Gore Athurva Methods for detecting aneuploidy
AU2015259024B2 (en) 2014-05-16 2021-07-01 Illumina, Inc. Nucleic acid synthesis techniques
CN110038653A (zh) 2014-05-27 2019-07-23 伊鲁米那股份有限公司 包括基本仪器和可拆卸盒的用于生物化学分析的系统和方法
CA3221500A1 (en) 2014-06-03 2015-12-10 Illumina, Inc. Compositions, systems, and methods for detecting events using tethers anchored to or adjacent to nanopores
US20150353989A1 (en) 2014-06-09 2015-12-10 Illumina Cambridge Limited Sample preparation for nucleic acid amplification
EP3715468A1 (en) 2014-06-13 2020-09-30 Illumina Cambridge Limited Methods and compositions for preparing sequencing libraries
US10829814B2 (en) 2014-06-19 2020-11-10 Illumina, Inc. Methods and compositions for single cell genomics
US10017759B2 (en) 2014-06-26 2018-07-10 Illumina, Inc. Library preparation of tagged nucleic acid
ES2788949T3 (es) 2014-06-27 2020-10-23 Illumina Inc Polimerasas modificadas para la incorporación mejorada de análogos de nucleótidos
DK3161152T3 (en) 2014-06-30 2019-03-25 Illumina Inc Methods and compositions using one-sided transposition
ES2697428T3 (es) 2014-07-15 2019-01-23 Illumina Inc Dispositivo electrónico activado bioquímicamente
SG11201702066UA (en) 2014-07-21 2017-04-27 Illumina Inc Polynucleotide enrichment using crispr-cas systems
KR102585730B1 (ko) 2014-08-08 2023-10-10 퀀텀-에스아이 인코포레이티드 수신된 광자들의 시간 비닝을 위한 집적 디바이스
US10174363B2 (en) 2015-05-20 2019-01-08 Quantum-Si Incorporated Methods for nucleic acid sequencing
GB201414098D0 (en) * 2014-08-08 2014-09-24 Illumina Cambridge Ltd Modified nucleotide linkers
EP4421188A3 (en) 2014-08-08 2024-10-23 Quantum-Si Incorporated Integrated device with external light source for probing, detecting, and analyzing molecules
CN106796175B (zh) 2014-08-08 2021-01-05 宽腾矽公司 用于探测、检测和分析分子的光学系统和检测芯片
CN107076739B (zh) 2014-08-21 2018-12-25 伊卢米纳剑桥有限公司 可逆表面官能化
FR3025201B1 (fr) 2014-09-02 2018-10-12 Dna Script Nucleotides modifies pour la synthese d'acides nucleiques, un kit renfermant de tels nucleotides et leur utilisation pour la production de genes ou sequences d'acides nucleiques synthetiques
US11408024B2 (en) 2014-09-10 2022-08-09 Molecular Loop Biosciences, Inc. Methods for selectively suppressing non-target sequences
WO2016040602A1 (en) 2014-09-11 2016-03-17 Epicentre Technologies Corporation Reduced representation bisulfite sequencing using uracil n-glycosylase (ung) and endonuclease iv
US10633694B2 (en) 2014-09-12 2020-04-28 Illumina, Inc. Compositions, systems, and methods for detecting the presence of polymer subunits using chemiluminescence
AU2015318017B2 (en) 2014-09-18 2022-02-03 Illumina, Inc. Methods and systems for analyzing nucleic acid sequencing data
EP3224595A4 (en) 2014-09-24 2018-06-13 Good Start Genetics, Inc. Process control for increased robustness of genetic assays
ES2747227T3 (es) 2014-09-30 2020-03-10 Illumina Inc Polimerasas modificadas para la incorporación mejorada de análogos de nucleótidos
US10487357B2 (en) 2014-10-03 2019-11-26 Life Technologies Corporation Methods of nucleic acid analysis using terminator nucleotides
US10544455B2 (en) 2014-10-03 2020-01-28 Life Technologies Corporation Sequencing methods, compositions and systems using terminator nucleotides
US10118173B2 (en) 2014-10-09 2018-11-06 Illumina, Inc. Method and device for separating immiscible liquids to effectively isolate at least one of the liquids
US9897791B2 (en) 2014-10-16 2018-02-20 Illumina, Inc. Optical scanning systems for in situ genetic analysis
IL299976B2 (en) 2014-10-17 2024-11-01 Illumina Cambridge Ltd Contiguity preserving transposition
EP3209781B8 (en) 2014-10-20 2020-01-15 Molecular Assemblies Inc. Modified template-independent enzymes for polydeoxynucleotide synthesis
CN107074904B (zh) 2014-10-23 2022-12-23 深圳华大智造科技股份有限公司 信号约束测序(scs)和用于信号约束测序的核苷酸类似物
CA3188941A1 (en) 2014-10-31 2016-05-06 Illumina Cambridge Limited Polymers and dna copolymer coatings
US10000799B2 (en) 2014-11-04 2018-06-19 Boreal Genomics, Inc. Methods of sequencing with linked fragments
WO2016073237A1 (en) 2014-11-05 2016-05-12 Illumina Cambridge Limited Reducing dna damage during sample preparation and sequencing using siderophore chelators
GB201419731D0 (en) 2014-11-05 2014-12-17 Illumina Cambridge Ltd Sequencing from multiple primers to increase data rate and density
ES2802405T3 (es) 2014-11-11 2021-01-19 Illumina Cambridge Ltd Métodos y matrices para producir y secuenciar agrupaciones monoclonales de ácido nucleico
CN107109495B (zh) 2014-11-11 2021-12-24 伊鲁米那股份有限公司 使用crispr-cas系统的多核苷酸扩增
CN112782140A (zh) 2014-12-03 2021-05-11 伊索普莱西斯公司 细胞分泌特征的分析和筛选
CN114438172B (zh) 2014-12-15 2025-02-21 亿明达股份有限公司 用于基底上的单分子放置的组合物和方法
CA3010579A1 (en) 2015-01-06 2016-07-14 Good Start Genetics, Inc. Screening for structural variants
CA2975739C (en) 2015-02-10 2022-12-06 Illumina, Inc. Methods and compositions for analyzing cellular components
US10576471B2 (en) 2015-03-20 2020-03-03 Illumina, Inc. Fluidics cartridge for use in the vertical or substantially vertical position
US10300452B2 (en) 2015-03-24 2019-05-28 Pacific Biosciences Of California, Inc. Methods and compositions for single molecule composition loading
EP4089398B1 (en) 2015-03-24 2024-09-04 Illumina, Inc. Carrier assemblies and systems for imaging samples for biological or chemical analysis
US20180100192A1 (en) 2015-03-31 2018-04-12 lllumina Cambridge Limited Surface concatemerization of templates
EP4119677B1 (en) 2015-04-10 2023-06-28 Spatial Transcriptomics AB Spatially distinguished, multiplex nucleic acid analysis of biological specimens
US10900030B2 (en) 2015-04-14 2021-01-26 Illumina, Inc. Structured substrates for improving detection of light emissions and methods relating to the same
US10844428B2 (en) 2015-04-28 2020-11-24 Illumina, Inc. Error suppression in sequenced DNA fragments using redundant reads with unique molecular indices (UMIS)
WO2016182984A1 (en) * 2015-05-08 2016-11-17 Centrillion Technology Holdings Corporation Disulfide-linked reversible terminators
CN107810415B (zh) 2015-05-11 2021-06-22 亿明达股份有限公司 用于发现和分析治疗剂的平台
RU2685953C1 (ru) 2015-05-12 2019-04-23 Иллюмина, Инк. Полевое устройство и способы секвенирования нуклеиновых кислот
AU2016269785B2 (en) 2015-05-29 2019-02-28 Illumina Cambridge Limited Enhanced utilization of surface primers in clusters
ES2927121T3 (es) 2015-05-29 2022-11-02 Illumina Inc Portamuestras y sistema de ensayo para realizar reacciones designadas
WO2016196358A1 (en) 2015-05-29 2016-12-08 Epicentre Technologies Corporation Methods of analyzing nucleic acids
IL255758B (en) 2015-06-03 2022-07-01 Illumina Inc Compositions, systems, and methods for sequencing polynucleotides using tethers anchored to polymerases adjacent to nanopores
US10344336B2 (en) 2015-06-09 2019-07-09 Life Technologies Corporation Methods, systems, compositions, kits, apparatus and computer-readable media for molecular tagging
US10889861B2 (en) 2015-06-17 2021-01-12 The Translational Genomics Research Institute Systems and methods for obtaining biological molecules from a sample
WO2017007757A1 (en) 2015-07-06 2017-01-12 Illumina, Inc. Balanced ac modulation for driving droplet operations electrodes
EP3878974A1 (en) 2015-07-06 2021-09-15 Illumina Cambridge Limited Sample preparation for nucleic acid amplification
US10808282B2 (en) 2015-07-07 2020-10-20 Illumina, Inc. Selective surface patterning via nanoimprinting
ES2945607T3 (es) 2015-07-17 2023-07-04 Illumina Inc Láminas de polímero para aplicaciones de secuenciación
EP3325966B1 (en) 2015-07-20 2021-01-20 Sentilus Holdco, LLC Chips, detectors, and methods of making and using the same
DK3329012T3 (da) 2015-07-27 2021-10-11 Illumina Inc Rumlig kortlægning af nukleinsyresekvensinformation
AU2016298541B2 (en) 2015-07-30 2019-10-31 Illumina, Inc. Orthogonal deblocking of nucleotides
PL3334839T3 (pl) 2015-08-14 2021-08-02 Illumina, Inc. Systemy i sposoby wykorzystujące czujniki reagujące na pole magnetyczne do określania informacji genetycznej
US10976334B2 (en) 2015-08-24 2021-04-13 Illumina, Inc. In-line pressure accumulator and flow-control system for biological or chemical assays
HK1250757A1 (zh) 2015-08-28 2019-01-11 Illumina, Inc. 來自單細胞的核酸序列分析
US10906044B2 (en) 2015-09-02 2021-02-02 Illumina Cambridge Limited Methods of improving droplet operations in fluidic systems with a filler fluid including a surface regenerative silane
US10647981B1 (en) 2015-09-08 2020-05-12 Bio-Rad Laboratories, Inc. Nucleic acid library generation methods and compositions
US10450598B2 (en) 2015-09-11 2019-10-22 Illumina, Inc. Systems and methods for obtaining a droplet having a designated concentration of a substance-of-interest
WO2017058953A1 (en) 2015-09-28 2017-04-06 The Trustees Of Columbia University In The City Of New York Design and synthesis of novel disulfide linker based nucleotides as reversible terminators for dna sequencing by synthesis
JP6657721B2 (ja) 2015-09-30 2020-03-04 ソニー株式会社 細胞の分析方法、細胞分析用チップ、細胞分析用試薬、細胞分析用キット及び細胞分析用装置
US10577643B2 (en) 2015-10-07 2020-03-03 Illumina, Inc. Off-target capture reduction in sequencing techniques
US10465232B1 (en) 2015-10-08 2019-11-05 Trace Genomics, Inc. Methods for quantifying efficiency of nucleic acid extraction and detection
HK1254546A1 (zh) 2015-10-22 2019-07-19 亿明达股份有限公司 用於流體裝置的填充流體
US11421264B2 (en) 2015-11-06 2022-08-23 IsoPlexis Corporation Thiol-containing cleave reagents and oxidative wash
US10273539B2 (en) 2015-11-06 2019-04-30 Qiagen Sciences, Llc Methods of using nucleotide analogues
JP6942362B2 (ja) 2015-11-09 2021-09-29 ラディアクション リミテッド 放射線遮蔽装置およびその用途
WO2017087724A1 (en) 2015-11-17 2017-05-26 Omniome, Inc. Methods for determining sequence profiles
WO2017087696A1 (en) 2015-11-18 2017-05-26 Pacific Biosciences Of California, Inc. Methods and compositions for loading of polymerase complexes
CN116218970A (zh) 2015-11-19 2023-06-06 赛纳生物科技(北京)有限公司 用于获得和校正目标多核苷酸的序列信息的方法
CA3006985C (en) 2015-12-01 2021-11-09 Illumina, Inc. Digital microfluidic system for single-cell isolation and characterization of analytes
EP3390657B1 (en) 2015-12-17 2020-09-16 Illumina, Inc. Distinguishing methylation levels in complex biological samples
EP3400444A4 (en) 2016-01-04 2019-10-16 QuantuMDx Group Limited DESIGN, SYNTHESIS AND USE OF SYNTHETIC NUCLEOTIDES COMPRISING SPECIFIC CHARGE LABELS
TW201730563A (zh) 2016-01-11 2017-09-01 伊路米納有限公司 具有顯微螢光計、流體系統和流動單元閂鎖夾持模組的檢測裝置
AU2017219894B2 (en) 2016-02-17 2021-12-09 Tesseract Health, Inc. Sensor and device for lifetime imaging and detection applications
US10059992B2 (en) 2016-03-24 2018-08-28 Illumina, Inc. Photonic superlattice-based devices and compositions for use in luminescent imaging, and methods of using the same
US10961568B2 (en) 2016-03-28 2021-03-30 Boreal Genomics, Inc. Linked target capture
US10961573B2 (en) 2016-03-28 2021-03-30 Boreal Genomics, Inc. Linked duplex target capture
ES2861350T3 (es) 2016-03-28 2021-10-06 Illumina Inc Micromatrices de planos múltiples
US11225687B2 (en) * 2016-04-04 2022-01-18 The Trustees Of Columbia University In The City Of New York DNA sequencing by synthesis with nucleotide analogues and Raman detection
US11326206B2 (en) 2016-04-07 2022-05-10 Pacific Biosciences Of California, Inc. Methods of quantifying target nucleic acids and identifying sequence variants
CN109312396A (zh) 2016-04-07 2019-02-05 伊鲁米那股份有限公司 用于构建标准化核酸文库的方法和系统
KR20180099903A (ko) 2016-04-22 2018-09-05 옴니옴 인코포레이티드 뉴클레오타이드-특이적 삼원 복합체 형성의 향상된 검출을 사용하는 핵산 서열 결정 방법 및 시스템
SG10202010314UA (en) 2016-04-22 2020-11-27 Illumina Inc Photonic stucture-based devices and compositions for use in luminescent imaging of multiple sites within a pixel, and methods of using the same
US10597643B2 (en) 2016-04-29 2020-03-24 Omniome, Inc. Polymerases engineered to reduce nucleotide-independent DNA binding
JP6915939B2 (ja) 2016-04-29 2021-08-11 オムニオム インコーポレイテッドＯｍｎｉｏｍｅ， Ｉｎｃ． 核酸配列決定方法
WO2017190018A1 (en) 2016-04-29 2017-11-02 Omniome, Inc. Sequencing method employing ternary complex destabilization to identify cognate nucleotides
EP3455372B1 (en) 2016-05-11 2019-10-30 Illumina, Inc. Polynucleotide enrichment and amplification using argonaute systems
SG11201809913PA (en) * 2016-05-16 2018-12-28 Nanostring Technologies Inc Methods for detecting target nucleic acids in a sample
KR102171865B1 (ko) 2016-05-18 2020-10-29 일루미나, 인코포레이티드 패턴화된 소수성 표면을 사용한 자가 조립된 패터닝
EP3463381A4 (en) * 2016-05-23 2020-01-01 The Trustees of Columbia University in the City of New York NUCLEOTIDE DERIVATIVES AND THEIR METHODS OF USE
FI3464628T3 (fi) 2016-06-06 2024-05-03 Redvault Biosciences Lp Kohdereportterikonstruktit ja niiden käyttö
CA3026773C (en) 2016-07-22 2022-10-18 Oregon Health & Science University Single cell whole genome libraries and combinatorial indexing methods of making thereof
KR102230444B1 (ko) 2016-08-15 2021-03-23 옴니옴 인코포레이티드 핵산을 시퀀싱하기 위한 방법 및 시스템
WO2018034780A1 (en) 2016-08-15 2018-02-22 Omniome, Inc. Sequencing method for rapid identification and processing of cognate nucleotide pairs
EP3510398A1 (en) 2016-09-12 2019-07-17 Isoplexis Corporation System and methods for multiplexed analysis of cellular and other immunotherapeutics
CA3037917C (en) 2016-09-22 2024-05-28 Illumina, Inc. Somatic copy number variation detection
WO2018064116A1 (en) 2016-09-28 2018-04-05 Illumina, Inc. Methods and systems for data compression
CN111781139B (zh) 2016-10-14 2023-09-12 亿明达股份有限公司 夹盒组件
EP3529380B1 (en) 2016-10-19 2022-06-29 Illumina, Inc. Methods for chemical ligation of nucleic acids
JP7091325B2 (ja) * 2016-11-04 2022-06-27 キアゲン サイエンシーズ， エルエルシー チオール含有切断試薬および酸化的洗浄液
JP7348066B2 (ja) 2016-11-11 2023-09-20 アイソプレキシス コーポレイション 単一細胞のゲノム、トランスクリプトームおよびプロテオームの同時解析のための組成物および方法
BR112019009949A2 (pt) 2016-11-16 2019-08-20 Illumina Inc método implantado por computador para validar chamadas de variante e sistema para validar chamadas de variante
GB201619458D0 (en) 2016-11-17 2017-01-04 Spatial Transcriptomics Ab Method for spatial tagging and analysing nucleic acids in a biological specimen
JP6730525B2 (ja) 2016-11-21 2020-07-29 ナノストリング テクノロジーズ，インコーポレイティド 化学組成物とそれを利用する方法
US11525783B2 (en) 2016-11-22 2022-12-13 IsoPlexis Corporation Systems, devices and methods for cell capture and methods of manufacture thereof
BR112018015871B1 (pt) 2016-12-09 2021-12-07 The Broad Institute, Inc. Sistema, método e dispositivo para detectar a presença de uma sequência alvo de ácido nucleico em uma amostra
JP7048609B2 (ja) 2016-12-09 2022-04-05 ボリアル ジェノミクス， インコーポレイテッド 連結型ライゲーション
WO2018119057A2 (en) 2016-12-22 2018-06-28 Illumina, Inc. Array including sequencing primer and non-sequencing entity
US20180195115A1 (en) 2016-12-22 2018-07-12 Illumina, Inc. Arrays with quality control tracers
MX2019006705A (es) 2016-12-22 2019-11-08 Illumina Inc Arreglos que incluyen una película de resina y una capa de polímero con patrón.
EP4224530A1 (en) 2016-12-22 2023-08-09 Quantum-si Incorporated Integrated photodetector with direct binning pixel
US11248254B2 (en) 2016-12-30 2022-02-15 Omniome, Inc. Method and system employing distinguishable polymerases for detecting ternary complexes and identifying cognate nucleotides
GB201704754D0 (en) 2017-01-05 2017-05-10 Illumina Inc Kinetic exclusion amplification of nucleic acid libraries
WO2018128544A1 (en) 2017-01-06 2018-07-12 Agendia N.V. Biomarkers for selecting patient groups, and uses thereof.
KR102521547B1 (ko) 2017-01-06 2023-04-14 일루미나, 인코포레이티드 페이징 보정
EP3568490B1 (en) 2017-01-10 2021-03-10 Omniome, Inc. Polymerases engineered to reduce nucleotide-independent dna binding
CA3045498C (en) 2017-01-17 2021-07-13 Illumina, Inc. Oncogenic splice variant determination
AU2018210188B2 (en) 2017-01-18 2023-11-09 Illumina, Inc. Methods and systems for generation and error-correction of unique molecular index sets with heterogeneous molecular lengths
GB201700983D0 (en) 2017-01-20 2017-03-08 Life Tech As Polymeric particles
WO2018136117A1 (en) 2017-01-20 2018-07-26 Omniome, Inc. Allele-specific capture of nucleic acids
WO2018136487A1 (en) 2017-01-20 2018-07-26 Omniome, Inc. Process for cognate nucleotide detection in a nucleic acid sequencing workflow
CA3050852C (en) 2017-01-20 2021-03-09 Omniome, Inc. Genotyping by polymerase binding
GB201701686D0 (en) 2017-02-01 2017-03-15 Illunina Inc System & method with fiducials having offset layouts
GB201701688D0 (en) 2017-02-01 2017-03-15 Illumia Inc System and method with fiducials in non-recliner layouts
EP3576868A4 (en) 2017-02-01 2021-03-17 Illumina, Inc. SYSTEM AND METHOD WITH MARKS RESPONDING TO MULTIPLE EXCITATION FREQUENCIES
GB201701689D0 (en) 2017-02-01 2017-03-15 Illumia Inc System and method with fiducials of non-closed shapes
US11492666B2 (en) 2017-02-15 2022-11-08 Pacific Biosciences Of California, Inc. Distinguishing sequences by detecting polymerase dissociation
EP3494235A1 (en) 2017-02-17 2019-06-12 Stichting VUmc Swarm intelligence-enhanced diagnosis and therapy selection for cancer using tumor- educated platelets
MX392295B (es) 2017-02-21 2025-03-24 Illumina Inc Etiquetado utilizando transposomas inmovilizados con ligadores
EP3596099B1 (en) 2017-03-06 2024-07-24 Singular Genomics Systems, Inc. Nucleic acid sequencing-by-synthesis (sbs) methods that combine sbs cycle steps
US11174515B2 (en) 2017-03-15 2021-11-16 The Broad Institute, Inc. CRISPR effector system based diagnostics
US11021740B2 (en) 2017-03-15 2021-06-01 The Broad Institute, Inc. Devices for CRISPR effector system based diagnostics
US11104937B2 (en) 2017-03-15 2021-08-31 The Broad Institute, Inc. CRISPR effector system based diagnostics
BR112019019087A2 (pt) 2017-03-15 2020-05-12 The Broad Institute, Inc. Diagnóstico baseado em sistema efetor de crispr para detecção de vírus
KR102676067B1 (ko) 2017-03-17 2024-06-18 퍼시픽 바이오사이언스 오브 캘리포니아, 인크. 시퀀싱 및 고 해상도 이미징
WO2018175258A1 (en) 2017-03-20 2018-09-27 Illumina, Inc. Methods and compositions for preparing nuclelic acid libraries
CN110446791B (zh) 2017-03-24 2024-04-16 生命技术公司 多核苷酸衔接子及其使用方法
US12018325B2 (en) 2017-03-28 2024-06-25 The Trustees Of Columbia University In The City Of New York 3′-O-modified nucleotide analogues with different cleavable linkers for attaching fluorescent labels to the base for DNA sequencing by synthesis
EP3607087A4 (en) 2017-04-04 2020-12-30 Omniome, Inc. FLUIDIC APPARATUS AND USEFUL METHODS FOR CHEMICAL AND BIOLOGICAL REACTIONS
CN110785492B (zh) 2017-04-23 2024-04-30 伊鲁米纳剑桥有限公司 用于改进编索引的核酸文库中的样品鉴定的组合物和方法
CN111094584B (zh) 2017-04-23 2024-11-26 伊鲁米那股份有限公司 用于改进编索引的核酸文库中的样品鉴定的组合物和方法
CN110785497A (zh) 2017-04-23 2020-02-11 伊鲁米纳剑桥有限公司 用于改进编索引的核酸文库中的样品鉴定的组合物和方法
DK3615691T3 (da) 2017-04-23 2021-07-26 Illumina Inc Sammensætninger og fremgangsmåder til forbedring af prøveidentifikation i indekserede nukleinsyrebiblioteker
US9951385B1 (en) 2017-04-25 2018-04-24 Omniome, Inc. Methods and apparatus that increase sequencing-by-binding efficiency
US10161003B2 (en) 2017-04-25 2018-12-25 Omniome, Inc. Methods and apparatus that increase sequencing-by-binding efficiency
WO2018204423A1 (en) 2017-05-01 2018-11-08 Illumina, Inc. Optimal index sequences for multiplex massively parallel sequencing
DK3622089T3 (da) 2017-05-08 2024-10-14 Illumina Inc Fremgangsmåde til sekventering under anvendelse af universelle korte adaptere til indeksering af polynukleotidprøver
ES3014783T3 (en) 2017-06-07 2025-04-24 Univ Oregon Health & Science Single cell whole genome libraries for methylation sequencing
US11542540B2 (en) 2017-06-16 2023-01-03 Life Technologies Corporation Control nucleic acids, and compositions, kits, and uses thereof
CN111032882A (zh) 2017-06-20 2020-04-17 伊鲁米那股份有限公司 解决扩增反应中低效的方法和组合物
GB201711219D0 (en) 2017-07-12 2017-08-23 Illumina Cambridge Ltd Short pendant arm linkers for nucleotides in sequencing applications
CA3070407C (en) 2017-07-18 2022-08-09 Omniome, Inc. Method of chemically modifying plastic surfaces
CN111133293A (zh) 2017-07-24 2020-05-08 宽腾矽公司 手持式大规模并行生物光电仪器
BR112020000697A2 (pt) 2017-07-24 2020-07-14 Quantum-Si Incorporated composições reagentes marcadas de alta intensidade e métodos para sequenciamento
US20200202977A1 (en) 2017-07-31 2020-06-25 Illumina, Inc. Sequencing system with multiplexed biological sample aggregation
DK3662083T3 (da) 2017-08-01 2024-11-18 Illumina Inc Rumlig indeksering af genetisk materiale og biblioteksfremstilling under anvendelse af hydrogelperler og flowceller
ES3006009T3 (en) 2017-08-01 2025-03-17 Illumina Inc Hydrogel beads for nucleotide sequencing
ES2941083T3 (es) 2017-08-01 2023-05-16 Mgi Tech Co Ltd Método de secuenciación de ácidos nucleicos
WO2019023924A1 (en) 2017-08-01 2019-02-07 Helitec Limited METHODS FOR ENRICHMENT AND DETERMINATION OF TARGET NUCLEOTIDE SEQUENCES
US11390856B2 (en) 2017-08-07 2022-07-19 Dna Script Variants of family a DNA polymerase and uses thereof
KR20200075814A (ko) 2017-08-15 2020-06-26 옴니옴 인코포레이티드 화학적 및 생물학적 분석물의 검출에 유용한 스캐닝 장치 및 방법
CN110678559B (zh) * 2017-08-31 2023-09-15 深圳华大智造科技股份有限公司 一种核酸探针以及一种核酸测序方法
US11447818B2 (en) 2017-09-15 2022-09-20 Illumina, Inc. Universal short adapters with variable length non-random unique molecular identifiers
US11640848B2 (en) 2017-09-20 2023-05-02 Regeneron Pharmaceuticals, Inc. Immunotherapy methods for patients whose tumors carry a high passenger gene mutation burden
EP4589017A2 (en) 2017-10-06 2025-07-23 10x Genomics, Inc. Rna templated ligation
MY182749A (en) 2017-10-16 2021-02-05 Illumina Inc Semi-supervised learning for training an ensemble of deep convolutional neural networks
KR102526103B1 (ko) 2017-10-16 2023-04-26 일루미나, 인코포레이티드 심층 학습 기반 스플라이스 부위 분류
AU2018353136B2 (en) 2017-10-19 2022-05-19 Pacific Biosciences Of California, Inc. Simultaneous background reduction and complex stabilization in binding assay workflows
KR102735452B1 (ko) 2017-11-06 2024-11-27 일루미나, 인코포레이티드 핵산 색인 기술
NZ759171A (en) 2017-11-16 2022-05-27 Illumina Inc Systems and methods for determining microsatellite instability
EP3733683A4 (en) 2017-11-30 2022-04-06 GeneMind Biosciences Company Limited NUCLEOSIDE ANALOGUES, METHOD OF MANUFACTURE AND USE
EP3718113B1 (en) 2017-11-30 2025-07-02 Illumina, Inc. Validation methods and systems for sequence variant calls
JP6868541B2 (ja) * 2017-12-05 2021-05-12 イルミナ ケンブリッジ リミテッド 修飾ヌクレオシドまたは修飾ヌクレオチド
JP7458678B2 (ja) 2017-12-29 2024-04-01 ノーティラス・サブシディアリー・インコーポレイテッド タンパク質同定のためのデコーディングアプローチ方法
US11378544B2 (en) 2018-01-08 2022-07-05 Illumina, Inc. High-throughput sequencing with semiconductor-based detection
WO2019135007A1 (en) 2018-01-08 2019-07-11 Dna Script Variants of terminal deoxynucleotidyl transferase and uses thereof
US10752887B2 (en) 2018-01-08 2020-08-25 Dna Script Variants of terminal deoxynucleotidyl transferase and uses thereof
AU2019205797A1 (en) 2018-01-08 2019-12-19 Illumina, Inc. Systems and devices for high-throughput sequencing with semiconductor-based detection
IL283427B2 (en) 2018-01-15 2023-10-01 Illumina Inc Empirical variant score (evs)-based variant caller
US10844430B2 (en) 2018-01-24 2020-11-24 Qiagen Sciences, Llc DNA sequencing reaction additive
JP7527961B2 (ja) 2018-01-29 2024-08-05 ザ・ブロード・インスティテュート・インコーポレイテッド Ｃｒｉｓｐｒエフェクター系に基づく診断
AU2019217824A1 (en) 2018-02-06 2020-07-30 Omniome, Inc. Compositions and techniques for nucleic acid primer extension
CN111094589A (zh) 2018-02-13 2020-05-01 伊鲁米纳公司 使用水凝胶珠的dna测序
EP3752631A4 (en) 2018-02-16 2021-11-10 Illumina, Inc. CHARGE-LABEL NUCLEOTIDES AND THEIR METHODS OF USE
WO2019164977A1 (en) 2018-02-21 2019-08-29 Singular Genomics Systems, Inc. Fluorinated nucleotides and uses thereof
US12188086B2 (en) 2018-03-15 2025-01-07 The Trustees Of Columbia University In The City Of New York Nucleotide analogues and use thereof for nucleic acid sequencing and analysis preliminary class
WO2019195225A1 (en) 2018-04-02 2019-10-10 Illumina, Inc. Compositions and methods for making controls for sequence-based genetic testing
CA3096621A1 (en) 2018-04-09 2019-10-17 The Board Of Trustees Of The Leland Stanford Junior University Method of in situ gene sequencing
US20190318806A1 (en) 2018-04-12 2019-10-17 Illumina, Inc. Variant Classifier Based on Deep Neural Networks
AU2019255987A1 (en) 2018-04-19 2020-12-10 Pacific Biosciences Of California, Inc. Improving accuracy of base calls in nucleic acid sequencing methods
KR102657606B1 (ko) 2018-04-20 2024-04-15 일루미나, 인코포레이티드 단일 세포를 캡슐화하는 방법, 캡슐화된 세포 및 이의 용도
WO2019209946A1 (en) 2018-04-25 2019-10-31 Qiagen Sciences Llc Sequential paired-end sequencing
EP3784800B1 (en) 2018-04-26 2023-05-03 Pacific Biosciences of California, Inc. Methods and compositions for stabilizing nucleic acid-nucleotide-polymerase complexes
SG11202011274YA (en) 2018-05-14 2020-12-30 Nanostring Technologies Inc Chemical compositions and methods of using same
BR112019027944A2 (pt) 2018-05-15 2020-12-01 Illumina, Inc. composições e métodos para clivagem química e desproteção de oligonucleotídeos ligados à superfície
KR102640255B1 (ko) 2018-05-17 2024-02-27 일루미나, 인코포레이티드 감소된 증폭 편향을 갖는 고속-대량 단일 세포 서열분석
WO2019226689A1 (en) 2018-05-22 2019-11-28 Axbio Inc. Methods, systems, and compositions for nucleic acid sequencing
EP3802880B1 (en) 2018-05-25 2024-10-23 Illumina, Inc. Circulating rna signatures specific to preeclampsia
US11821023B2 (en) 2018-05-30 2023-11-21 Generation Biotech, Llc Method for capturing long polynucleotides for sequencing specific gnomic regions
US11180794B2 (en) 2018-05-31 2021-11-23 Omniome, Inc. Methods and compositions for capping nucleic acids
CN112166201A (zh) 2018-05-31 2021-01-01 欧姆尼欧美公司 核酸测序中的增加的信噪比
IL272234B1 (en) 2018-06-04 2025-06-01 Illumina Inc High-throughput single-cell transcriptome libraries and methods for their production and use
EP3811610A1 (en) 2018-06-22 2021-04-28 Quantum-Si Incorporated Integrated photodetector with charge storage bin of varied detection time
CN108822173B (zh) * 2018-06-26 2021-04-02 合肥华纳生物医药科技有限公司 一种荧光标记可剪切核苷酸、合成方法及其在dna测序中的用途
NL2021377B1 (en) 2018-07-03 2020-01-08 Illumina Inc Interposer with first and second adhesive layers
US12073922B2 (en) 2018-07-11 2024-08-27 Illumina, Inc. Deep learning-based framework for identifying sequence patterns that cause sequence-specific errors (SSEs)
KR20210031935A (ko) 2018-07-13 2021-03-23 퀀텀-에스아이 인코포레이티드 이중접합가능한 표지 및 사용 방법
CN113056327A (zh) 2018-07-23 2021-06-29 Dna斯克瑞普特公司 核酸链的大规模平行酶促合成
CA3107165A1 (en) 2018-07-24 2020-01-30 Omniome, Inc. Serial formation of ternary complex species
WO2020022891A2 (en) 2018-07-26 2020-01-30 Stichting Vumc Biomarkers for atrial fibrillation
WO2020028194A1 (en) 2018-07-30 2020-02-06 Readcoor, Inc. Methods and systems for sample processing or analysis
WO2020028882A1 (en) 2018-08-03 2020-02-06 10X Genomics, Inc. Methods and systems to minimize barcode exchange
CN113286884A (zh) 2018-08-07 2021-08-20 博德研究所 新型cas12b酶和系统
JP7431802B2 (ja) 2018-08-15 2024-02-15 イルミナ インコーポレイテッド ライブラリー富化を改善するための組成物および方法
WO2020039261A1 (en) 2018-08-23 2020-02-27 Boreal Genomics, Inc. Linked target capture and ligation
EP3844308A1 (en) 2018-08-28 2021-07-07 10X Genomics, Inc. Resolving spatial arrays
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
SG11202102029TA (en) 2018-08-28 2021-03-30 10X Genomics Inc Methods for generating spatially barcoded arrays
US20230011240A1 (en) 2018-09-11 2023-01-12 Singular Genomics Systems, Inc. Modified archaeal family b polymerases
EP3853358A1 (en) 2018-09-17 2021-07-28 Omniome, Inc. Engineered polymerases for improved sequencing
EP3853382A4 (en) 2018-09-19 2022-06-22 Apton Biosystems, Inc. Densely-packed analyte layers and detection methods
CN113195511A (zh) * 2018-09-28 2021-07-30 生捷科技控股公司 二硫键连接的可逆终止剂
US20210396756A1 (en) 2018-10-03 2021-12-23 The Broad Institute, Inc. Crispr effector system based diagnostics for hemorrhagic fever detection
WO2020073274A1 (zh) * 2018-10-11 2020-04-16 深圳华大智造极创科技有限公司 对多核苷酸进行测序的方法
JP6888123B2 (ja) 2018-10-15 2021-06-16 イルミナ インコーポレイテッド 深層畳み込みニューラルネットワークを事前訓練するための深層学習ベースの技術
US12145960B2 (en) 2018-10-25 2024-11-19 Singular Genomics Systems, Inc. Nucleotide analogues
US11085036B2 (en) 2018-10-26 2021-08-10 Illumina, Inc. Modulating polymer beads for DNA processing
JP7515413B2 (ja) 2018-10-31 2024-07-12 イラミーナ インコーポレーテッド ポリメラーゼ、組成物、および使用方法
AU2019379287A1 (en) 2018-11-14 2021-06-03 Dna Script Terminal deoxynucleotidyl transferase variants and uses thereof
WO2020101795A1 (en) 2018-11-15 2020-05-22 Omniome, Inc. Electronic detection of nucleic acid structure
NL2022043B1 (en) 2018-11-21 2020-06-03 Akershus Univ Hf Tagmentation-Associated Multiplex PCR Enrichment Sequencing
KR20210098432A (ko) 2018-11-30 2021-08-10 일루미나, 인코포레이티드 단일 검정을 이용한 다수의 분석물의 분석
EP3891304A1 (en) 2018-12-04 2021-10-13 Omniome, Inc. Mixed-phase fluids for nucleic acid sequencing and other analytical assays
KR20210098844A (ko) 2018-12-05 2021-08-11 일루미나, 인코포레이티드 중합효소, 조성물, 및 사용 방법
EP3891305A1 (en) 2018-12-05 2021-10-13 Illumina Cambridge Limited Methods and compositions for cluster generation by bridge amplification
WO2020123309A1 (en) 2018-12-10 2020-06-18 10X Genomics, Inc. Resolving spatial arrays by proximity-based deconvolution
GB201820300D0 (en) 2018-12-13 2019-01-30 10X Genomics Inc Method for spatial tagging and analysing genomic DNA in a biological specimen
GB201820341D0 (en) 2018-12-13 2019-01-30 10X Genomics Inc Method for transposase-mediated spatial tagging and analysing genomic DNA in a biological specimen
US11268091B2 (en) 2018-12-13 2022-03-08 Dna Script Sas Direct oligonucleotide synthesis on cells and biomolecules
AU2019400090A1 (en) 2018-12-14 2021-01-07 Illumina Cambridge Limited Decreasing phasing with unlabeled nucleotides during sequencing
CN112654714B (zh) 2018-12-17 2024-10-11 伊卢米纳剑桥有限公司 用于测序的引物寡核苷酸
AU2019411266B2 (en) 2018-12-17 2025-06-26 Illumina Cambridge Limited Compositions for use in polyunucleotide sequencing
CA3103633A1 (en) 2018-12-18 2020-06-25 Illumina Cambridge Limited Methods and compositions for paired end sequencing using a single surface primer
ES2965222T3 (es) 2018-12-19 2024-04-11 Illumina Inc Métodos para mejorar la prioridad de clonalidad de agrupamientos de polinucleótidos
EP3899032A2 (en) 2018-12-20 2021-10-27 Omniome, Inc. Temperature control for analysis of nucleic acids and other analytes
US11293061B2 (en) 2018-12-26 2022-04-05 Illumina Cambridge Limited Sequencing methods using nucleotides with 3′ AOM blocking group
JP7548588B2 (ja) 2019-01-02 2024-09-10 ラディアクション リミテッド 放射線保護装置およびそのための材料
JP7498970B2 (ja) 2019-01-02 2024-06-13 ラディアクション リミテッド 患者頭部保護デバイス
EP4538389A2 (en) 2019-01-03 2025-04-16 Ncan Genomics, Inc. Linked target capture
CA3124763A1 (en) 2019-01-03 2020-07-09 Dna Script One pot synthesis of sets of oligonucleotides
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
WO2020146397A1 (en) 2019-01-08 2020-07-16 Singular Genomics Systems, Inc. Nucleotide cleavable linkers and uses thereof
KR20210114918A (ko) 2019-01-11 2021-09-24 일루미나 케임브리지 리미티드 복합체 표면-결합 트랜스포좀 복합체
EP3914603A1 (en) 2019-01-23 2021-12-01 Quantum-Si Incorporated High intensity labeled reactant compositions and methods for sequencing
WO2020163630A1 (en) 2019-02-06 2020-08-13 Singular Genomics Systems, Inc. Compositions and methods for nucleic acid sequencing
US11359221B2 (en) 2019-02-12 2022-06-14 Dna Script Sas Efficient product cleavage in template-free enzymatic synthesis of polynucleotides
US11499189B2 (en) 2019-02-14 2022-11-15 Pacific Biosciences Of California, Inc. Mitigating adverse impacts of detection systems on nucleic acids and other biological analytes
CA3130693A1 (en) 2019-02-19 2020-08-27 Ultima Genomics, Inc. Linkers and methods for optical detection and sequencing
WO2020172444A1 (en) 2019-02-20 2020-08-27 Omniome, Inc. Scanning apparatus and methods for detecting chemical and biological analytes
CN114174531A (zh) 2019-02-28 2022-03-11 10X基因组学有限公司 用空间条码化寡核苷酸阵列对生物分析物进行概况分析
BR112021006234A2 (pt) 2019-03-01 2021-09-28 Illumina, Inc. Bibliotecas de células únicas e núcleos únicos de alto rendimento e métodos de preparo e uso
EP3938538A1 (en) 2019-03-15 2022-01-19 10X Genomics, Inc. Methods for using spatial arrays for single cell sequencing
US11347965B2 (en) 2019-03-21 2022-05-31 Illumina, Inc. Training data generation for artificial intelligence-based sequencing
NL2023312B1 (en) 2019-03-21 2020-09-28 Illumina Inc Artificial intelligence-based base calling
NL2023316B1 (en) 2019-03-21 2020-09-28 Illumina Inc Artificial intelligence-based sequencing
US11210554B2 (en) 2019-03-21 2021-12-28 Illumina, Inc. Artificial intelligence-based generation of sequencing metadata
NL2023310B1 (en) 2019-03-21 2020-09-28 Illumina Inc Training data generation for artificial intelligence-based sequencing
NL2023311B9 (en) 2019-03-21 2021-03-12 Illumina Inc Artificial intelligence-based generation of sequencing metadata
WO2020205296A1 (en) 2019-03-21 2020-10-08 Illumina, Inc. Artificial intelligence-based generation of sequencing metadata
NL2023314B1 (en) 2019-03-21 2020-09-28 Illumina Inc Artificial intelligence-based quality scoring
EP3887542A1 (en) 2019-03-22 2021-10-06 10X Genomics, Inc. Three-dimensional spatial analysis
US11738336B2 (en) 2019-04-12 2023-08-29 Western Digital Technologies, Inc. Spin torque oscillator (STO) sensors used in nucleic acid sequencing arrays and detection schemes for nucleic acid sequencing
WO2020210370A1 (en) 2019-04-12 2020-10-15 Roche Sequencing Solutions, Inc. Nucleic acid sequencing by synthesis using magnetic sensor arrays
US11579217B2 (en) 2019-04-12 2023-02-14 Western Digital Technologies, Inc. Devices and methods for frequency- and phase-based detection of magnetically-labeled molecules using spin torque oscillator (STO) sensors
US11112468B2 (en) 2019-04-12 2021-09-07 Western Digital Technologies, Inc. Magnetoresistive sensor array for molecule detection and related detection schemes
US11609208B2 (en) 2019-04-12 2023-03-21 Western Digital Technologies, Inc. Devices and methods for molecule detection based on thermal stabilities of magnetic nanoparticles
US11327073B2 (en) 2019-04-12 2022-05-10 Western Digital Technologies, Inc. Thermal sensor array for molecule detection and related detection schemes
US11593649B2 (en) 2019-05-16 2023-02-28 Illumina, Inc. Base calling using convolutions
US11423306B2 (en) 2019-05-16 2022-08-23 Illumina, Inc. Systems and devices for characterization and performance analysis of pixel-based sequencing
WO2020243579A1 (en) 2019-05-30 2020-12-03 10X Genomics, Inc. Methods of detecting spatial heterogeneity of a biological sample
US11644406B2 (en) 2019-06-11 2023-05-09 Pacific Biosciences Of California, Inc. Calibrated focus sensing
WO2021003191A1 (en) 2019-07-02 2021-01-07 Yifat Jonathan Deployable radiation shield cover
CA3125241A1 (en) 2019-07-12 2021-01-21 Illumina Cambridge Limited Compositions and methods for preparing nucleic acid sequencing libraries using crispr/cas9 immobilized on a solid support
AU2020312787A1 (en) 2019-07-12 2021-06-17 Illumina Cambridge Limited Nucleic acid library preparation using electrophoresis
WO2021011803A1 (en) 2019-07-16 2021-01-21 Omniome, Inc. Synthetic nucleic acids having non-natural structures
US10656368B1 (en) 2019-07-24 2020-05-19 Omniome, Inc. Method and system for biological imaging using a wide field objective lens
EP4004222A1 (en) 2019-07-30 2022-06-01 DNA Script Template-free enzymatic synthesis of polynucleotides using poly(a) and poly(u) polymerases
WO2021018921A1 (en) 2019-08-01 2021-02-04 Dna Script Increasing long-sequence yields in template-free enzymatic synthesis of polynucleotides.
EP4047098A4 (en) * 2019-08-20 2023-06-07 EGI Tech (Shen Zhen) Co., Limited PROCEDURE FOR SEQUENCING POLYNUCLEOTIDS BASED ON OPTICAL SIGNAL DYNAMICS OF A LUMINESCENT LABEL AND SECONDARY LUMINESCENT SIGNAL
CN114555818A (zh) 2019-09-09 2022-05-27 Dna斯克瑞普特公司 使用光可切割连接的无模板酶促多核苷酸合成
WO2021050681A1 (en) 2019-09-10 2021-03-18 Omniome, Inc. Reversible modification of nucleotides
US20220290245A1 (en) 2019-09-11 2022-09-15 The United States Of America, As Represented By The Secretary, Department Of Health And Human Servic Cancer detection and classification
US11512295B2 (en) 2019-09-12 2022-11-29 Singular Genomics Systems, Inc. Modified thermoccocus polymerases
US11208682B2 (en) 2019-09-13 2021-12-28 Western Digital Technologies, Inc. Enhanced optical detection for nucleic acid sequencing using thermally-dependent fluorophore tags
AU2020352503A1 (en) 2019-09-23 2022-05-19 Dna Script Increasing long-sequence yields in template-free enzymatic synthesis of polynucleotides
US11287422B2 (en) 2019-09-23 2022-03-29 Element Biosciences, Inc. Multivalent binding composition for nucleic acid analysis
CN114761992B (zh) 2019-10-01 2023-08-08 10X基因组学有限公司 用于识别组织样品中的形态学模式的系统和方法
MX2022004657A (es) * 2019-10-18 2022-07-21 Univ Leland Stanford Junior Secuenciacion de tejidos intactos a escala clinica e industrial.
CN114599795A (zh) 2019-10-18 2022-06-07 加利福尼亚太平洋生物科学股份有限公司 用于对核酸加帽的方法和组合物
EP4025711A2 (en) 2019-11-08 2022-07-13 10X Genomics, Inc. Enhancing specificity of analyte binding
WO2021091611A1 (en) 2019-11-08 2021-05-14 10X Genomics, Inc. Spatially-tagged analyte capture agents for analyte multiplexing
US20210139867A1 (en) 2019-11-08 2021-05-13 Omniome, Inc. Engineered polymerases for improved sequencing by binding
EP4058590A1 (en) 2019-11-13 2022-09-21 DNA Script High efficiency template-free enzymatic synthesis of polynucleotides
CN115004260A (zh) 2019-11-18 2022-09-02 10X基因组学有限公司 用于组织分类的系统和方法
US20210155982A1 (en) 2019-11-21 2021-05-27 10X Genomics, Inc. Pipeline for spatial analysis of analytes
JP2023501760A (ja) 2019-11-22 2023-01-19 イルミナ インコーポレイテッド 子癇前症に特異的な循環ｒｎａシグネチャー
US11747329B2 (en) 2019-11-22 2023-09-05 Western Digital Technologies, Inc. Magnetic gradient concentrator/reluctance detector for molecule detection
DE202019106694U1 (de) 2019-12-02 2020-03-19 Omniome, Inc. System zur Sequenzierung von Nukleinsäuren in Fluidschaum
DE202019106695U1 (de) 2019-12-02 2020-03-19 Omniome, Inc. System zur Sequenzierung von Nukleinsäuren in Fluidschaum
CN113631721A (zh) 2019-12-04 2021-11-09 因美纳有限公司 用于检测血浆中dna病原体的dna测序文库的制备
WO2021118349A1 (en) 2019-12-10 2021-06-17 Prinses Máxima Centrum Voor Kinderoncologie B.V. Methods of typing germ cell tumors
IL293820A (en) 2019-12-12 2022-08-01 Dna Script Chimeric terminal deoxynucleotidyl transferases for template-free enzymatic synthesis of polynucleotides
CN115135770A (zh) 2019-12-16 2022-09-30 Dna斯克瑞普特公司 使用无歧化作用末端脱氧核苷酸转移酶变体的多核苷酸无模板酶学合成
EP4077344B1 (en) * 2019-12-18 2025-07-23 F. Hoffmann-La Roche AG Methods of sequencing by synthesis using a consecutive labeling scheme
SG11202109486QA (en) 2019-12-19 2021-09-29 Illumina Inc High-throughput single-cell libraries and methods of making and of using
GB201919032D0 (en) 2019-12-20 2020-02-05 Cartana Ab Method of detecting an analyte
US11747262B2 (en) 2019-12-23 2023-09-05 Singular Genomics Systems, Inc. Flow cell carrier and methods of use
US11498078B2 (en) 2019-12-23 2022-11-15 Singular Genomics Systems, Inc. Flow cell receiver and methods of use
EP4081656A1 (en) 2019-12-23 2022-11-02 10X Genomics, Inc. Compositions and methods for using fixed biological samples in partition-based assays
ES2946357T3 (es) 2019-12-23 2023-07-17 10X Genomics Inc Métodos para el análisis espacial usando ligación con molde de ARN
WO2021133685A1 (en) 2019-12-23 2021-07-01 Singular Genomics Systems, Inc. Methods for long read sequencing
AU2020416718A1 (en) 2019-12-31 2022-07-07 Singular Genomics Systems, Inc. Polynucleotide barcodes for long read sequencing
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US20210230681A1 (en) 2020-01-24 2021-07-29 10X Genomics, Inc. Methods for spatial analysis using proximity ligation
US11821035B1 (en) 2020-01-29 2023-11-21 10X Genomics, Inc. Compositions and methods of making gene expression libraries
WO2021155063A1 (en) 2020-01-29 2021-08-05 Readcoor, Llc Compositions and methods for analyte detection
US12076701B2 (en) 2020-01-31 2024-09-03 10X Genomics, Inc. Capturing oligonucleotides in spatial transcriptomics
US12110541B2 (en) 2020-02-03 2024-10-08 10X Genomics, Inc. Methods for preparing high-resolution spatial arrays
US12110548B2 (en) 2020-02-03 2024-10-08 10X Genomics, Inc. Bi-directional in situ analysis
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
US12112833B2 (en) 2020-02-04 2024-10-08 10X Genomics, Inc. Systems and methods for index hopping filtering
WO2021158511A1 (en) 2020-02-04 2021-08-12 Omniome, Inc. Flow cells and methods for their manufacture and use
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
US12129516B2 (en) 2020-02-07 2024-10-29 10X Genomics, Inc. Quantitative and automated permeabilization performance evaluation for spatial transcriptomics
US11835462B2 (en) 2020-02-11 2023-12-05 10X Genomics, Inc. Methods and compositions for partitioning a biological sample
US12281357B1 (en) 2020-02-14 2025-04-22 10X Genomics, Inc. In situ spatial barcoding
US12215379B2 (en) 2020-02-17 2025-02-04 10X Genomics, Inc. In situ analysis of chromatin interaction
US11807851B1 (en) 2020-02-18 2023-11-07 Ultima Genomics, Inc. Modified polynucleotides and uses thereof
JP2023515108A (ja) 2020-02-20 2023-04-12 イルミナ インコーポレイテッド 人工知能ベースの多対多ベースコール
US20210265016A1 (en) 2020-02-20 2021-08-26 Illumina, Inc. Data Compression for Artificial Intelligence-Based Base Calling
US20210265015A1 (en) 2020-02-20 2021-08-26 Illumina, Inc. Hardware Execution and Acceleration of Artificial Intelligence-Based Base Caller
US12354008B2 (en) 2020-02-20 2025-07-08 Illumina, Inc. Knowledge distillation and gradient pruning-based compression of artificial intelligence-based base caller
JP2023514749A (ja) 2020-02-21 2023-04-07 １０エックス ジェノミクス インコーポレイテッド 統合型インサイチュ空間的アッセイのための方法および組成物
CN115461143A (zh) 2020-02-21 2022-12-09 10X基因组学有限公司 用于样品处理的组合物、方法和系统
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
CN115151652B (zh) 2020-02-25 2025-02-07 Dna斯克瑞普特公司 用于酶促合成多核苷酸的方法和设备
US11034942B1 (en) 2020-02-27 2021-06-15 Singular Genomics Systems, Inc. Modified pyrococcus polymerases and uses thereof
US11926863B1 (en) 2020-02-27 2024-03-12 10X Genomics, Inc. Solid state single cell method for analyzing fixed biological cells
CA3173798A1 (en) 2020-03-03 2021-09-10 Pacific Biosciences Of California, Inc. Methods and compositions for sequencing double stranded nucleic acids
US11768175B1 (en) 2020-03-04 2023-09-26 10X Genomics, Inc. Electrophoretic methods for spatial analysis
US12188085B2 (en) 2020-03-05 2025-01-07 10X Genomics, Inc. Three-dimensional spatial transcriptomics with sequencing readout
EP4114978A4 (en) 2020-03-06 2024-07-03 Singular Genomics Systems, Inc. LINKED PAIRED STRAND SEQUENCING
CN115297964A (zh) 2020-03-10 2022-11-04 西部数据技术公司 用于核酸定序的磁性传感器阵列以及制造及使用其的方法
WO2021202403A1 (en) 2020-03-30 2021-10-07 Illumina, Inc. Methods and compositions for preparing nucleic acid libraries
EP4139446A1 (en) 2020-04-20 2023-03-01 DNA Script Terminal deoxynucleotidyl transferase variants and uses thereof
TWI803855B (zh) * 2020-04-21 2023-06-01 美商西方數位科技公司 用於定序核酸之系統及裝置、定序複數個ｓ個核酸股之方法及減輕由於使用單分子感測器陣列之核酸定序程序而產生之定序資料之錯誤之方法
WO2021216708A1 (en) 2020-04-22 2021-10-28 10X Genomics, Inc. Methods for spatial analysis using targeted rna depletion
WO2021216998A1 (en) 2020-04-24 2021-10-28 Singular Genomics Systems, Inc. Modified nucleotides and uses thereof
EP4143344A1 (en) 2020-04-27 2023-03-08 Agendia N.V. Treatment of her2 negative, mammaprint high risk 2 breast cancer
US11188778B1 (en) 2020-05-05 2021-11-30 Illumina, Inc. Equalization-based image processing and spatial crosstalk attenuator
WO2021224677A1 (en) 2020-05-05 2021-11-11 Akershus Universitetssykehus Hf Compositions and methods for characterizing bowel cancer
WO2021225886A1 (en) 2020-05-05 2021-11-11 Omniome, Inc. Compositions and methods for modifying polymerase-nucleic acid complexes
US20230207059A1 (en) 2020-05-08 2023-06-29 Illumina, Inc. Genome sequencing and detection techniques
JP2023526062A (ja) 2020-05-12 2023-06-20 イルミナ インコーポレイテッド 組換え末端デオキシヌクレオチジルトランスフェラーゼを使用して修飾塩基を有する核酸の生成
EP4153775B1 (en) 2020-05-22 2024-07-24 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
EP4153776B1 (en) 2020-05-22 2025-03-05 10X Genomics, Inc. Spatial analysis to detect sequence variants
WO2021242834A1 (en) 2020-05-26 2021-12-02 10X Genomics, Inc. Method for resetting an array
US12265079B1 (en) 2020-06-02 2025-04-01 10X Genomics, Inc. Systems and methods for detecting analytes from captured single biological particles
WO2021247543A2 (en) 2020-06-02 2021-12-09 10X Genomics, Inc. Nucleic acid library methods
AU2021283184A1 (en) 2020-06-02 2023-01-05 10X Genomics, Inc. Spatial transcriptomics for antigen-receptors
US12031177B1 (en) 2020-06-04 2024-07-09 10X Genomics, Inc. Methods of enhancing spatial resolution of transcripts
WO2021252375A1 (en) 2020-06-08 2021-12-16 The Broad Institute, Inc. Single cell combinatorial indexing from amplified nucleic acids
EP4162074B1 (en) 2020-06-08 2024-04-24 10X Genomics, Inc. Methods of determining a surgical margin and methods of use thereof
WO2021252617A1 (en) 2020-06-09 2021-12-16 Illumina, Inc. Methods for increasing yield of sequencing libraries
ES2999535T3 (en) 2020-06-10 2025-02-26 10X Genomics Inc Methods for determining a location of an analyte in a biological sample
US11935311B2 (en) 2020-06-11 2024-03-19 Nautilus Subsidiary, Inc. Methods and systems for computational decoding of biological, chemical, and physical entities
US12209273B2 (en) 2020-06-12 2025-01-28 10X Genomics, Inc. Nucleic acid assays using click chemistry bioconjugation
JP2023531620A (ja) 2020-06-16 2023-07-25 ディーエヌエー スクリプト 酵素的ポリヌクレオチド合成のためのシステム、装置及びキット
MA58991B1 (fr) 2020-06-22 2024-09-30 Illumina Cambridge Limited Nucléosides et nucléotides avec groupe de blocage acétal 3'
CN116034166A (zh) 2020-06-25 2023-04-28 10X基因组学有限公司 Dna甲基化的空间分析
EP4172364A1 (en) 2020-06-30 2023-05-03 Illumina, Inc. Catalytically controlled sequencing by synthesis to produce scarless dna
BR112022026806A2 (pt) 2020-07-02 2023-04-25 Illumina Inc Método para calibrar a eficiência de semeadura de biblioteca de ácidos nucleicos em células de fluxo
US12168801B1 (en) 2020-07-02 2024-12-17 10X Genomics, Inc. Hybrid/capture probe designs for full-length cDNA
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
US12209280B1 (en) 2020-07-06 2025-01-28 10X Genomics, Inc. Methods of identifying abundance and location of an analyte in a biological sample using second strand synthesis
US11981960B1 (en) 2020-07-06 2024-05-14 10X Genomics, Inc. Spatial analysis utilizing degradable hydrogels
AU2021306281A1 (en) 2020-07-08 2023-01-05 Illumina, Inc. Beads as transposome carriers
WO2022015600A2 (en) 2020-07-13 2022-01-20 Singular Genomics Systems, Inc. Methods of sequencing complementary polynucleotides
US20230313255A1 (en) 2020-07-15 2023-10-05 Dna Script Massively Parallel Enzymatic Synthesis of Polynucleotides
US11981964B2 (en) 2020-07-28 2024-05-14 Illumina Cambridge Limited Substituted coumarin dyes and uses as fluorescent labels
AU2021320307A1 (en) 2020-08-06 2023-02-16 Illumina Cambridge Limited Preparation of RNA and DNA sequencing libraries using bead-linked transposomes
US12297499B2 (en) 2020-08-17 2025-05-13 10X Genomics, Inc. Multicomponent nucleic acid probes for sample analysis
US20220049303A1 (en) 2020-08-17 2022-02-17 Readcoor, Llc Methods and systems for spatial mapping of genetic variants
WO2022040176A1 (en) 2020-08-18 2022-02-24 Illumina, Inc. Sequence-specific targeted transposition and selection and sorting of nucleic acids
US11981958B1 (en) 2020-08-20 2024-05-14 10X Genomics, Inc. Methods for spatial analysis using DNA capture
US20220067489A1 (en) 2020-08-28 2022-03-03 Illumina, Inc. Detecting and Filtering Clusters Based on Artificial Intelligence-Predicted Base Calls
US11200446B1 (en) 2020-08-31 2021-12-14 Element Biosciences, Inc. Single-pass primary analysis
WO2022053610A1 (en) 2020-09-11 2022-03-17 Illumina Cambridge Limited Methods of enriching a target sequence from a sequencing library using hairpin adaptors
EP4211508A4 (en) 2020-09-14 2024-10-23 Singular Genomics Systems, Inc. METHODS AND SYSTEMS FOR MULTI-DIMENSIONAL IMAGING
US20230351619A1 (en) 2020-09-18 2023-11-02 10X Genomics, Inc. Sample handling apparatus and image registration methods
EP4217476A1 (en) 2020-09-22 2023-08-02 DNA Script Stabilized n-terminally truncated terminal deoxynucleotidyl transferase variants and uses thereof
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
AU2021366658A1 (en) 2020-10-21 2023-06-22 Illumina Cambridge Limited Sequencing templates comprising multiple inserts and compositions and methods for improving sequencing throughput
WO2022087485A1 (en) 2020-10-22 2022-04-28 Singular Genomics Systems, Inc. Nucleic acid circularization and amplification on a surface
WO2022090323A1 (en) 2020-10-29 2022-05-05 Dna Script Enzymatic synthesis of polynucleotide probes
EP4200444A4 (en) 2020-10-30 2024-09-25 Singular Genomics Systems, Inc. METHODS AND COMPOSITIONS FOR REDUCING NUCLEOTIDE IMPURITIES CONTENT
US12071667B2 (en) 2020-11-04 2024-08-27 10X Genomics, Inc. Sequence analysis using meta-stable nucleic acid molecules
US20220145345A1 (en) 2020-11-11 2022-05-12 Microsoft Technology Licensing, Llc Spatial control of polynucleotide synthesis by strand capping
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
WO2022118862A1 (ja) * 2020-12-01 2022-06-09 国立大学法人九州大学 生体分子構造検出用プローブ、生体分子構造検出用キット、及び生体分子構造の検出方法
CN116157869A (zh) 2020-12-02 2023-05-23 伊鲁米纳软件公司 用于检测遗传改变的系统和方法
EP4012046A1 (en) 2020-12-11 2022-06-15 10X Genomics, Inc. Methods and compositions for multimodal in situ analysis
US12099178B2 (en) 2020-12-16 2024-09-24 Singular Genomics Systems, Inc. Kinematic imaging system
CA3205944A1 (en) 2020-12-21 2022-06-30 Singular Genomics Systems, Inc. Systems and methods for multicolor imaging
WO2022140028A1 (en) 2020-12-21 2022-06-30 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
US20220298568A1 (en) 2021-01-13 2022-09-22 Pacific Biosciences Of California, Inc. Surface structuring with colloidal assembly
US12060603B2 (en) 2021-01-19 2024-08-13 10X Genomics, Inc. Methods for internally controlled in situ assays using padlock probes
EP4284945B1 (en) 2021-01-26 2024-10-23 10X Genomics, Inc. Nucleic acid analog probes for in situ analysis
CN117043352A (zh) 2021-01-29 2023-11-10 伊鲁米纳公司 使用多核苷酸改善流通池的接种效率的方法、组合物和试剂盒
CA3208854A1 (en) 2021-02-04 2022-08-11 Illumina, Inc. Long indexed-linked read generation on transposome bound beads
WO2022170212A1 (en) 2021-02-08 2022-08-11 Singular Genomics Systems, Inc. Methods and compositions for sequencing complementary polynucleotides
WO2022174054A1 (en) 2021-02-13 2022-08-18 The General Hospital Corporation Methods and compositions for in situ macromolecule detection and uses thereof
EP4421491A3 (en) 2021-02-19 2024-11-27 10X Genomics, Inc. Method of using a modular assay support device
US12275984B2 (en) 2021-03-02 2025-04-15 10X Genomics, Inc. Sequential hybridization and quenching
WO2022187366A1 (en) 2021-03-03 2022-09-09 10X Genomics, Inc. Analyte detection in situ using nucleic acid origami
US11884977B2 (en) 2021-03-12 2024-01-30 Singular Genomics Systems, Inc. Nanoarrays and methods of use thereof
EP4263868A4 (en) 2021-03-12 2024-11-27 Singular Genomics Systems, Inc. NANONETWORKS AND METHODS OF USE THEREOF
WO2022197754A1 (en) 2021-03-16 2022-09-22 Illumina Software, Inc. Neural network parameter quantization for base calling
ES3008686T3 (en) 2021-03-18 2025-03-24 10X Genomics Inc Multiplex capture of gene and protein expression from a biological sample
US20220333178A1 (en) 2021-03-22 2022-10-20 Illumina Cambridge Limited Methods for improving nucleic acid cluster clonality
CA3214278A1 (en) 2021-03-29 2022-10-06 Illumina, Inc Improved methods of library preparation
BR112023019894A2 (pt) 2021-03-29 2023-11-14 Illumina Inc Composições e métodos para avaliar os danos ao dna em uma biblioteca e normalizar a distorção do tamanho do amplicon
MX2023010495A (es) 2021-03-30 2023-09-18 Illumina Inc Metodos mejorados de preparacion de genoteca y adn complementarios isotermicos.
WO2022212402A1 (en) 2021-03-31 2022-10-06 Illumina, Inc. Methods of preparing directional tagmentation sequencing libraries using transposon-based technology with unique molecular identifiers for error correction
WO2022212180A1 (en) 2021-03-31 2022-10-06 Illumina, Inc. Artificial intelligence-based base caller with contextual awareness
MX2023011660A (es) 2021-03-31 2023-12-11 Illumina Cambridge Ltd Tecnicas de secuenciacion de genoteca de acido nucleico con deteccion de dimero adaptador.
NL2031471B1 (en) 2021-04-02 2024-03-29 Dna Script Methods and kits for enzymatic synthesis of g4-prone polynucleotides
WO2022213027A1 (en) 2021-04-02 2022-10-06 Illumina, Inc. Machine-learning model for detecting a bubble within a nucleotide-sample slide for sequencing
EP4428246A3 (en) 2021-04-14 2024-11-27 10X Genomics, Inc. Methods of measuring mislocalization of an analyte
US12217829B2 (en) 2021-04-15 2025-02-04 Illumina, Inc. Artificial intelligence-based analysis of protein three-dimensional (3D) structures
FR3121936A1 (fr) 2021-04-20 2022-10-21 Naos Institute Of Life Science Methode de criblage d’ingredients et de compositions topiques ecobiologiques, et son utilisation pour realiser un soin cosmetique de restauration du microbiote cutane
US12070744B2 (en) 2021-04-22 2024-08-27 Illumina, Inc. Valve assemblies and related systems
EP4294920A4 (en) 2021-04-27 2025-01-22 Singular Genomics Systems, Inc. HIGH-DENSITY SEQUENCING AND MULTIPLEX PRIMING
CN117377775A (zh) 2021-04-29 2024-01-09 因美纳有限公司 用于核酸表征的扩增技术
WO2022235764A1 (en) 2021-05-05 2022-11-10 Singular Genomics Systems, Inc. Multiomic analysis device and methods of use thereof
EP4334475A1 (en) 2021-05-07 2024-03-13 Agendia N.V. Endocrine treatment of hormone receptor positive breast cancer typed as having a low risk of recurrence
EP4337789A1 (en) 2021-05-10 2024-03-20 Pacific Biosciences of California, Inc. Dna amplification buffer replenishment during rolling circle amplification
WO2022240764A1 (en) 2021-05-10 2022-11-17 Pacific Biosciences Of California, Inc. Single-molecule seeding and amplification on a surface
AU2022277632A1 (en) 2021-05-20 2023-11-09 Illumina Cambridge Limited Compositions and methods for sequencing by synthesis
CN117413068A (zh) 2021-05-28 2024-01-16 因美纳有限公司 用于核酸制备的寡核苷酸修饰的核苷酸类似物
US20220380838A1 (en) 2021-06-01 2022-12-01 10X Genomics, Inc. Methods and compositions for analyte detection and probe resolution
CN117751197A (zh) 2021-06-02 2024-03-22 10X基因组学有限公司 使用不对称可环化探针的样品分析
US20220403450A1 (en) 2021-06-03 2022-12-22 Illumina Software, Inc. Systems and methods for sequencing nucleotides using two optical channels
EP4347879B1 (en) 2021-06-03 2025-02-19 10X Genomics, Inc. Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis
WO2022265994A1 (en) 2021-06-15 2022-12-22 Illumina, Inc. Hydrogel-free surface functionalization for sequencing
US11236388B1 (en) 2021-06-17 2022-02-01 Element Biosciences, Inc. Compositions and methods for pairwise sequencing
US11859241B2 (en) 2021-06-17 2024-01-02 Element Biosciences, Inc. Compositions and methods for pairwise sequencing
IL309440A (en) 2021-06-18 2024-02-01 Element Biosciences Inc engineered polymerases
WO2022272260A1 (en) 2021-06-23 2022-12-29 Illumina, Inc. Compositions, methods, kits, cartridges, and systems for sequencing reagents
CN117546245A (zh) 2021-06-29 2024-02-09 因美纳有限公司 用于生成基因组坐标的置信度分类的机器学习模型
WO2023278184A1 (en) 2021-06-29 2023-01-05 Illumina, Inc. Methods and systems to correct crosstalk in illumination emitted from reaction sites
WO2023278609A1 (en) 2021-06-29 2023-01-05 Illumina, Inc. Self-learned base caller, trained using organism sequences
US20220415442A1 (en) 2021-06-29 2022-12-29 Illumina Software, Inc. Signal-to-noise-ratio metric for determining nucleotide-base calls and base-call quality
US20230005253A1 (en) 2021-07-01 2023-01-05 Illumina, Inc. Efficient artificial intelligence-based base calling of index sequences
US20230027409A1 (en) 2021-07-13 2023-01-26 Illumina, Inc. Methods and systems for real time extraction of crosstalk in illumination emitted from reaction sites
WO2023288225A1 (en) 2021-07-13 2023-01-19 10X Genomics, Inc. Methods for preparing polymerized matrix with controllable thickness
US11455487B1 (en) 2021-10-26 2022-09-27 Illumina Software, Inc. Intensity extraction and crosstalk attenuation using interpolation and adaptation for base calling
JP2024532004A (ja) 2021-07-19 2024-09-04 イルミナ インコーポレイテッド ベースコールのための補間及び適合による強度抽出
EP4373958A1 (en) 2021-07-23 2024-05-29 Illumina, Inc. Methods for preparing substrate surface for dna sequencing
US20230021577A1 (en) 2021-07-23 2023-01-26 Illumina Software, Inc. Machine-learning model for recalibrating nucleotide-base calls
CA3223746A1 (en) 2021-07-28 2023-02-02 Rohan PAUL Quality score calibration of basecalling systems
US12139751B2 (en) 2021-07-30 2024-11-12 10X Genomics, Inc. Circularizable probes for in situ analysis
EP4326900B1 (en) 2021-07-30 2024-09-04 10X Genomics, Inc. Methods and compositions for synchronizing reactions in situ
WO2023015192A1 (en) 2021-08-03 2023-02-09 10X Genomics, Inc. Nucleic acid concatemers and methods for stabilizing and/or compacting the same
WO2023014741A1 (en) 2021-08-03 2023-02-09 Illumina Software, Inc. Base calling using multiple base caller models
US12077789B2 (en) 2021-08-14 2024-09-03 Illumina, Inc. Polymerases, compositions, and methods of use
CN117858958A (zh) 2021-08-16 2024-04-09 10X基因组学有限公司 包含分割条形码区的探针和使用方法
CA3223390A1 (en) 2021-08-17 2023-02-23 Colin Brown Methods and compositions for identifying methylated cytosines
WO2023034076A1 (en) 2021-08-31 2023-03-09 Illumina, Inc. Flow cell with enhanced well imaging resolution
EP4396376A1 (en) 2021-09-01 2024-07-10 Illumina, Inc. Amplitude modulation for accelerated base calling
EP4509614A3 (en) 2021-09-01 2025-05-14 10X Genomics, Inc. Methods, compositions, and kits for blocking a capture probe on a spatial array
EP4396372A2 (en) 2021-09-03 2024-07-10 Singular Genomics Systems, Inc. Amplification oligonucleotides
EP4402682A1 (en) 2021-09-17 2024-07-24 Illumina, Inc. Automatically identifying failure sources in nucleotide sequencing from base-call-error patterns
EP4405954A1 (en) 2021-09-21 2024-07-31 Illumina, Inc. A graph reference genome and base-calling approach using imputed haplotypes
WO2023049212A2 (en) 2021-09-22 2023-03-30 Illumina, Inc. State-based base calling
CN118318049A (zh) 2021-09-28 2024-07-09 安序源有限公司 用于处理核酸样品的方法以及核酸样品的组合物
WO2023056328A2 (en) 2021-09-30 2023-04-06 Illumina, Inc. Solid supports and methods for depleting and/or enriching library fragments prepared from biosamples
US20230096386A1 (en) 2021-09-30 2023-03-30 Illumina Cambridge Limited Polynucleotide sequencing
CA3232183A1 (en) 2021-10-11 2023-04-20 Nautilus Subsidiary, Inc. Highly multiplexable analysis of proteins and proteomes
WO2023069927A1 (en) 2021-10-20 2023-04-27 Illumina, Inc. Methods for capturing library dna for sequencing
EP4423664A1 (en) 2021-10-25 2024-09-04 Singular Genomics Systems, Inc. Manipulating and detecting biological samples
EP4422792A1 (en) 2021-10-26 2024-09-04 Singular Genomics Systems, Inc. Multiplexed targeted amplification of polynucleotides
WO2023081485A1 (en) 2021-11-08 2023-05-11 Pacific Biosciences Of California, Inc. Stepwise sequencing of a polynucleotide with a homogenous reaction mixture
WO2023083999A2 (en) 2021-11-10 2023-05-19 Dna Script Novel terminal deoxynucleotidyl transferase (tdt) variants
WO2023085932A1 (en) 2021-11-10 2023-05-19 Omnigen B.V. Prediction of response following folfirinox treatment in cancer patients
WO2023086880A1 (en) 2021-11-10 2023-05-19 10X Genomics, Inc. Methods, compositions, and kits for determining the location of an analyte in a biological sample
EP4430175A2 (en) 2021-11-10 2024-09-18 DNA Script Novel terminal deoxynucleotidyl
EP4305195A2 (en) 2021-12-01 2024-01-17 10X Genomics, Inc. Methods, compositions, and systems for improved in situ detection of analytes and spatial analysis
EP4441743A1 (en) 2021-12-02 2024-10-09 Illumina, Inc. Generating cluster-specific-signal corrections for determining nucleotide-base calls
WO2023107622A1 (en) 2021-12-10 2023-06-15 Illumina, Inc. Parallel sample and index sequencing
WO2023122033A1 (en) 2021-12-20 2023-06-29 10X Genomics, Inc. Self-test for pathology/histology slide imaging device
EP4453250A1 (en) 2021-12-21 2024-10-30 Illumina, Inc. Wax-microsphere matrix compositions and methods of making and using the same
US20230215515A1 (en) 2021-12-23 2023-07-06 Illumina Software, Inc. Facilitating secure execution of external workflows for genomic sequencing diagnostics
WO2023122363A1 (en) 2021-12-23 2023-06-29 Illumina Software, Inc. Dynamic graphical status summaries for nucelotide sequencing
EP4423296A2 (en) 2021-12-27 2024-09-04 10X Genomics, Inc. Methods and compositions for rolling circle amplification
US20230207050A1 (en) 2021-12-28 2023-06-29 Illumina Software, Inc. Machine learning model for recalibrating nucleotide base calls corresponding to target variants
WO2023126457A1 (en) 2021-12-29 2023-07-06 Illumina Cambridge Ltd. Methods of nucleic acid sequencing using surface-bound primers
AU2022425440A1 (en) 2021-12-29 2024-07-11 Illumina, Inc. Automatically switching variant analysis model versions for genomic analysis applications
WO2023141154A1 (en) 2022-01-20 2023-07-27 Illumina Cambridge Limited Methods of detecting methylcytosine and hydroxymethylcytosine by sequencing
EP4466376A1 (en) 2022-01-21 2024-11-27 10X Genomics, Inc. Multiple readout signals for analyzing a sample
IL315106A (en) 2022-02-23 2024-10-01 Insitro Inc Pooled optical screening and transcriptional measurements of cells comprising barcoded genetic perturbations
EP4483372A1 (en) 2022-02-25 2025-01-01 Illumina, Inc. Calibration sequences for nucelotide sequencing
CA3224595A1 (en) 2022-02-25 2023-08-31 Steven Norberg Machine-learning models for detecting and adjusting values for nucleotide methylation levels
US20250188521A1 (en) 2022-03-10 2025-06-12 Singular Genomics Systems, Inc. Nucleic acid delivery scaffolds
AU2023236924A1 (en) 2022-03-15 2024-10-24 Illumina, Inc. Paired-end sequencing
WO2023183937A1 (en) 2022-03-25 2023-09-28 Illumina, Inc. Sequence-to-sequence base calling
WO2023192917A1 (en) 2022-03-29 2023-10-05 Nautilus Subsidiary, Inc. Integrated arrays for single-analyte processes
EP4505177A1 (en) 2022-04-01 2025-02-12 10x Genomics, Inc. Compositions and methods for targeted masking of autofluorescence
US20230323427A1 (en) 2022-04-06 2023-10-12 10X Genomics, Inc. Methods and compositions for multiplex cell analysis
AU2023248405A1 (en) 2022-04-07 2024-01-04 Illumina, Inc. Altered cytidine deaminases and methods of use
AU2023250540A1 (en) 2022-04-08 2024-01-18 Illumina, Inc. Aptamer dynamic range compression and detection techniques
WO2023212490A1 (en) 2022-04-25 2023-11-02 Nautilus Subsidiary, Inc. Systems and methods for assessing and improving the quality of multiplex molecular assays
EP4515547A1 (en) 2022-04-26 2025-03-05 Illumina, Inc. Machine-learning models for selecting oligonucleotide probes for array technologies
WO2023209606A1 (en) 2022-04-29 2023-11-02 Illumina Cambridge Limited Methods and systems for encapsulating lyophilised microspheres
US20240002902A1 (en) 2022-05-06 2024-01-04 10X Genomics, Inc. Analysis of antigen and antigen receptor interactions
EP4523215A1 (en) 2022-05-10 2025-03-19 Illumina Inc. Adaptive neural network for nucelotide sequencing
JP2025516589A (ja) 2022-05-11 2025-05-30 １０エックス ジェノミクス インコーポレイテッド インサイチュー配列決定のための組成物及び方法
WO2023225095A1 (en) 2022-05-18 2023-11-23 Illumina Cambridge Limited Preparation of size-controlled nucleic acid fragments
EP4526470A1 (en) 2022-05-19 2025-03-26 Agendia N.V. Prediction of response to immune therapy in breast cancer patients
EP4526471A1 (en) 2022-05-19 2025-03-26 Agendia N.V. Dna repair signature and prediction of response following cancer therapy
EP4532769A2 (en) 2022-06-03 2025-04-09 Illumina, Inc. Circulating rna biomarkers for preeclampsia
EP4540607A1 (en) 2022-06-17 2025-04-23 10X Genomics, Inc. Catalytic de-crosslinking of samples for in situ analysis
EP4544558A1 (en) 2022-06-24 2025-04-30 Illumina, Inc. Improving split-read alignment by intelligently identifying and scoring candidate split groups
EP4544554A1 (en) 2022-06-27 2025-04-30 Illumina, Inc. Improved human leukocyte antigen (hla) genotyping
EP4544555A1 (en) 2022-06-27 2025-04-30 Illumina, Inc. Generating and implementing a structural variation graph genome
US20230420075A1 (en) 2022-06-27 2023-12-28 Illumina Software, Inc. Accelerators for a genotype imputation model
WO2024015962A1 (en) 2022-07-15 2024-01-18 Pacific Biosciences Of California, Inc. Blocked asymmetric hairpin adaptors
WO2024026356A1 (en) 2022-07-26 2024-02-01 Illumina, Inc. Rapid single-cell multiomics processing using an executable file
CN119677856A (zh) 2022-08-12 2025-03-21 10X基因组学有限公司 Puma1聚合酶及其用途
WO2024040060A1 (en) 2022-08-16 2024-02-22 10X Genomics, Inc. Ap50 polymerases and uses thereof
WO2024039516A1 (en) 2022-08-19 2024-02-22 Illumina, Inc. Third dna base pair site-specific dna detection
WO2024059655A1 (en) 2022-09-15 2024-03-21 Nautilus Subsidiary, Inc. Characterizing accessibility of macromolecule structures
US20240177807A1 (en) 2022-09-16 2024-05-30 Illumina, Inc. Cluster segmentation and conditional base calling
US20240112753A1 (en) 2022-09-29 2024-04-04 Illumina, Inc. Target-variant-reference panel for imputing target variants
WO2024073047A1 (en) 2022-09-30 2024-04-04 Illumina, Inc. Cytidine deaminases and methods of use in mapping modified cytosine nucleotides
US20240120027A1 (en) 2022-09-30 2024-04-11 Illumina, Inc. Machine-learning model for refining structural variant calls
CN120153070A (zh) 2022-09-30 2025-06-13 因美纳有限公司 解旋酶-胞苷脱氨酶复合物及其使用方法
WO2024073043A1 (en) 2022-09-30 2024-04-04 Illumina, Inc. Methods of using cpg binding proteins in mapping modified cytosine nucleotides
US20240141427A1 (en) 2022-09-30 2024-05-02 Illumina, Inc. Polymerases, compositions, and methods of use
WO2024077096A1 (en) 2022-10-05 2024-04-11 Illumina, Inc. Integrating variant calls from multiple sequencing pipelines utilizing a machine learning architecture
EP4482987A2 (en) 2022-10-06 2025-01-01 Illumina, Inc. Probes for improving coronavirus sample surveillance
WO2024077202A2 (en) 2022-10-06 2024-04-11 Illumina, Inc. Probes for improving environmental sample surveillance
EP4482978A1 (en) 2022-10-06 2025-01-01 Illumina, Inc. Probes for depleting abundant small noncoding rna
WO2024081649A1 (en) 2022-10-11 2024-04-18 Illumina, Inc. Detecting and correcting methylation values from methylation sequencing assays
WO2024081869A1 (en) 2022-10-14 2024-04-18 10X Genomics, Inc. Methods for analysis of biological samples
WO2024083883A1 (en) 2022-10-19 2024-04-25 Dna Script Methods and products for removal of uracil containing polynucleotides
US20240167081A1 (en) 2022-11-08 2024-05-23 10X Genomics,Inc. Immobilization methods and compositions for in situ detection
WO2024107887A1 (en) 2022-11-16 2024-05-23 10X Genomics, Inc. Methods and compositions for assessing performance of in situ assays
US20240177802A1 (en) 2022-11-30 2024-05-30 Illumina, Inc. Accurately predicting variants from methylation sequencing data
WO2024118903A1 (en) 2022-11-30 2024-06-06 Illumina, Inc. Chemoenzymatic correction of false positive uracil transformations
WO2024121022A1 (en) 2022-12-05 2024-06-13 Dna Script Variants of poly(a) polymerase and uses thereof
WO2024129672A1 (en) 2022-12-12 2024-06-20 The Broad Institute, Inc. Trafficked rnas for assessment of cell-cell connectivity and neuroanatomy
WO2024129969A1 (en) 2022-12-14 2024-06-20 Illumina, Inc. Systems and methods for capture and enrichment of clustered beads on flow cell substrates
WO2024130031A1 (en) 2022-12-16 2024-06-20 Illumina, Inc. Boranes on solid supports
WO2024130203A1 (en) 2022-12-16 2024-06-20 10X Genomics, Inc. Methods and compositions for assessing performance
US20240212791A1 (en) 2022-12-21 2024-06-27 Illumina, Inc. Context-dependent base calling
WO2024137860A1 (en) 2022-12-21 2024-06-27 10X Genomics, Inc. Methods for tethering ribonucleic acids in biological samples
WO2024137765A1 (en) 2022-12-22 2024-06-27 Illumina, Inc. Transition-metal catalyst compositions and methods for sequencing by synthesis
AU2023409219A1 (en) 2022-12-22 2024-10-03 Illumina, Inc. Palladium catalyst compositions and methods for sequencing by synthesis
AU2023419253A1 (en) 2022-12-27 2024-10-03 Illumina, Inc. Methods of sequencing using 3´ allyl blocked nucleotides
WO2024141628A1 (en) 2022-12-31 2024-07-04 Dna Script Variable viscosity inks for inkjet delivery of enzyme reagents
US20240263219A1 (en) 2023-01-06 2024-08-08 10X Genomics, Inc. Methods and compositions for in situ analysis of variant sequences
CN119301271A (zh) 2023-01-06 2025-01-10 因美纳有限公司 通过聚合酶减少尿嘧啶
US20240241110A1 (en) 2023-01-12 2024-07-18 Nautilus Subsidiary, Inc. Characterization of glycans and glycoconjugates
WO2024153642A1 (en) 2023-01-16 2024-07-25 Dna Script Scarless template-free enzymatic synthesis of polynucleotides
WO2024153643A1 (en) 2023-01-16 2024-07-25 Dna Script Inkjet-assisted enzymatic nucleic acid synthesis
WO2024156896A2 (en) 2023-01-26 2024-08-02 Dna Script Enzymatic synthesis of polynucleotide probes
WO2024167954A1 (en) 2023-02-06 2024-08-15 Illumina, Inc. Determining and removing inter-cluster light interference
CN119630812A (zh) 2023-02-17 2025-03-14 因美纳有限公司 作为先兆子痫的生物标志物的细胞游离dna信号
CN119452097A (zh) 2023-03-10 2025-02-14 因美纳有限公司 适体检测技术
CN119452101A (zh) 2023-03-30 2025-02-14 因美纳公司 用于核酸测序的组合物和方法
WO2024206848A1 (en) 2023-03-30 2024-10-03 Illumina, Inc. Tandem repeat genotyping
US20240371469A1 (en) 2023-05-03 2024-11-07 Illumina, Inc. Machine learning model for recalibrating genotype calls from existing sequencing data files
WO2024229260A1 (en) 2023-05-03 2024-11-07 10X Genomics, Inc. Methods and compositions for spatial assay
WO2024249200A1 (en) 2023-05-26 2024-12-05 Illumina, Inc. Methods for preserving methylation status during clustering
US20240404624A1 (en) 2023-05-31 2024-12-05 Illumina, Inc. Structural variant alignment and variant calling by utilizing a structural-variant reference genome
WO2024249466A1 (en) 2023-05-31 2024-12-05 Illumina, Inc. False positive reduction by translesion polymerase repair
WO2024249591A1 (en) 2023-05-31 2024-12-05 Illumina, Inc. Methods for double-stranded sequencing by synthesis
WO2024249973A2 (en) 2023-06-02 2024-12-05 Illumina, Inc. Linking human genes to clinical phenotypes using graph neural networks
WO2024254003A1 (en) 2023-06-05 2024-12-12 Illumina, Inc. Identification and mapping of methylation sites
WO2024256604A1 (en) 2023-06-15 2024-12-19 Dna Script Salt addition during enzymatic polynucleotide synthesis
US20250002960A1 (en) 2023-06-26 2025-01-02 Pacific Biosciences Of California, Inc. Compositions and methods for nucleic acid extension
WO2025006565A1 (en) 2023-06-27 2025-01-02 Illumina, Inc. Variant calling with methylation-level estimation
WO2025006464A1 (en) 2023-06-30 2025-01-02 Illumina, Inc. Systems and methods of sequencing polynucleotides with alternative scatterplots
WO2025003434A1 (en) 2023-06-30 2025-01-02 Dna Script Nucleic acid synthesis on reusable support
WO2025006487A1 (en) 2023-06-30 2025-01-02 Illumina, Inc. Using flowcell spatial coordinates to link reads for improved genome analysis
WO2025006874A1 (en) 2023-06-30 2025-01-02 Illumina, Inc. Machine-learning model for recalibrating genotype calls corresponding to germline variants and somatic mosaic variants
WO2025006460A1 (en) 2023-06-30 2025-01-02 Illumina, Inc. Systems and methods of sequencing polynucleotides with modified bases
WO2025006466A1 (en) 2023-06-30 2025-01-02 Illumina, Inc. Systems and methods of sequencing polynucleotides with four labeled nucleotides
WO2025010160A1 (en) 2023-07-06 2025-01-09 Pacific Biosciences Of California, Inc. Methods and compositions for stabilizing concatemers
WO2025024672A1 (en) 2023-07-26 2025-01-30 Element Biosciences, Inc. Dna sequencing systems and use thereof
US12319956B2 (en) 2023-07-31 2025-06-03 10X Genomics, Inc. Methods and systems for targeted RNA cleavage and target RNA-primed rolling circle amplification
GB202312147D0 (en) 2023-08-08 2023-09-20 Syndex Bio Ltd Methylation method
WO2025049720A2 (en) 2023-08-31 2025-03-06 Illumina, Inc. Aptamer dynamic range compression and detection techniques
WO2025049331A2 (en) 2023-08-31 2025-03-06 Illumina, Inc. Aptamer detection techniques
WO2025054389A1 (en) 2023-09-07 2025-03-13 Illumina, Inc. Identification of methylated cytosine using landmarks
WO2025050463A1 (en) 2023-09-08 2025-03-13 The Regents Of The University Of Michigan MICRORNA-DERIVED RNAs AND POLYPEPTIDES AND USES THEREOF
WO2025059045A1 (en) 2023-09-12 2025-03-20 Illumina, Inc. Systems and methods for determining linkage of sequence reads on a flow cell
WO2025058517A1 (en) 2023-09-12 2025-03-20 Levels Diagnostics Holding B.V. Biomarkers for typing a sample of an individual for hepatocellular carcinoma.
WO2025059533A1 (en) 2023-09-13 2025-03-20 The Broad Institute, Inc. Crispr enzymes and systems
WO2025061942A1 (en) 2023-09-20 2025-03-27 Illumina, Inc. Sequencing error identification and correction
WO2025061922A1 (en) 2023-09-20 2025-03-27 Illumina, Inc. Methods for sequencing
WO2025062341A1 (en) 2023-09-20 2025-03-27 Element Biosciences, Inc. Solid-state and configurable optical test targets and flow cell devices
WO2025072368A1 (en) 2023-09-28 2025-04-03 Illumina, Inc. Capture and selective release of biological material
WO2025072800A2 (en) 2023-09-28 2025-04-03 Illumina, Inc. Altered cytidine deaminases and methods of use
US20250111899A1 (en) 2023-09-29 2025-04-03 Illumina, Inc. Predicting insert lengths using primary analysis metrics
WO2025072870A1 (en) 2023-09-29 2025-04-03 Illumina, Inc. Tracking and modifying cluster location on nucleotide-sample slides in real time
WO2025081064A2 (en) 2023-10-11 2025-04-17 Illumina, Inc. Thermophilic deaminase and methods for identifying modified cytosine
WO2025088024A1 (en) 2023-10-24 2025-05-01 Dna Script Novel terminal deoxynucleotidyl transferase (tdt) variants and uses thereof
WO2025090883A1 (en) 2023-10-27 2025-05-01 Illumina, Inc. Detecting variants in nucleotide sequences based on haplotype diversity
WO2025106629A1 (en) 2023-11-17 2025-05-22 Illumina, Inc. Structural variant detection using spatially linked reads
WO2025106431A1 (en) 2023-11-17 2025-05-22 Illumina, Inc. Determining structural variants
WO2025111463A1 (en) 2023-11-22 2025-05-30 10X Genomics, Inc. Methods for processing ribonucleic acids in biological samples
WO2025117738A1 (en) 2023-11-28 2025-06-05 Illumina, Inc. Methods of improving unique molecular index ligation efficiency
EP4567128A1 (en) 2023-12-07 2025-06-11 Max-Delbrück-Centrum für Molekulare Medizin in der Helmholtz-Gemeinschaft Improved method and means for spatial nucleic acid detection in-situ
WO2025123005A2 (en) 2023-12-07 2025-06-12 Illumina, Inc. Preparation and compositions of cot-1 nucleic acid
WO2025129074A2 (en) 2023-12-14 2025-06-19 Illumina, Inc. Indexing techniques for tagmented dna libraries
WO2025129133A1 (en) 2023-12-15 2025-06-19 Illumina, Inc. Minimal residual disease (mrd) models for determining likelihoods or probabilities of a subject comprising cancer
WO2025136998A1 (en) 2023-12-18 2025-06-26 Illumina, Inc. Using machine learning models for detecting minimum residual disease (mrd) in a subject
WO2025137222A1 (en) 2023-12-19 2025-06-26 Illumina, Inc. Methylation detection assay
US20250207203A1 (en) 2023-12-20 2025-06-26 10X Genomics, Inc. In situ detection of copy number variations in biological samples
WO2025136105A1 (en) 2023-12-20 2025-06-26 Stichting Amsterdam UMC Intestinal tissue-adherent microbial signatures predictive of response to anti-tnf-alpha in crohn's disease
WO2025137341A1 (en) 2023-12-20 2025-06-26 Illumina, Inc. Directly determining signal-to-noise-ratio metrics for accelerated convergence in determining nucleotide-base calls and base-call quality
WO2025137268A1 (en) 2023-12-20 2025-06-26 Pacific Biosciences Of California, Inc. Methods and compositions for reducing gc bias
US20250210141A1 (en) 2023-12-21 2025-06-26 Illumina, Inc. Enhanced mapping and alignment of nucleotide reads utilizing an improved haplotype data structure with allele-variant differences
WO2025136717A1 (en) 2023-12-22 2025-06-26 Illumina, Inc. Improving mapping resolution using spatial information of sequenced reads
US20250215493A1 (en) 2023-12-28 2025-07-03 Illumina, Inc. Nucleotides with enzymatically cleavable 3'-o-glycoside blocking groups for sequencing
US20250215491A1 (en) 2023-12-28 2025-07-03 Illumina, Inc. Sequencing with single-stranded binding proteins

Family Cites Families (97)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4711955A (en) 1981-04-17 1987-12-08 Yale University Modified nucleotides and methods of preparing and using same
US5175269A (en) 1984-01-30 1992-12-29 Enzo Diagnostics, Inc. Compound and detectable molecules having an oligo- or polynucleotide with modifiable reactive group
US5118605A (en) 1984-10-16 1992-06-02 Chiron Corporation Polynucleotide determination with selectable cleavage sites
US4824775A (en) 1985-01-03 1989-04-25 Molecular Diagnostics, Inc. Cells labeled with multiple Fluorophores bound to a nucleic acid carrier
US4772691A (en) 1985-06-05 1988-09-20 The Medical College Of Wisconsin, Inc. Chemically cleavable nucleotides
US4863849A (en) 1985-07-18 1989-09-05 New York Medical College Automatable process for sequencing nucleotide
US5151507A (en) 1986-07-02 1992-09-29 E. I. Du Pont De Nemours And Company Alkynylamino-nucleotides
SE8801070D0 (sv) 1988-03-23 1988-03-23 Pharmacia Ab Method for immobilizing a dna sequence on a solid support
US5174962A (en) 1988-06-20 1992-12-29 Genomyx, Inc. Apparatus for determining DNA sequences by mass spectrometry
GB8910880D0 (en) 1989-05-11 1989-06-28 Amersham Int Plc Sequencing method
US5547839A (en) 1989-06-07 1996-08-20 Affymax Technologies N.V. Sequencing of surface immobilized polymers utilizing microflourescence detection
JPH07111484B2 (ja) * 1989-06-26 1995-11-29 松下電器産業株式会社 プラスチック製光学部品の反射防止膜とその形成方法
US5302509A (en) 1989-08-14 1994-04-12 Beckman Instruments, Inc. Method for sequencing polynucleotides
WO1991006678A1 (en) 1989-10-26 1991-05-16 Sri International Dna sequencing
WO1993005183A1 (en) 1991-09-09 1993-03-18 Baylor College Of Medicine Method and device for rapid dna or rna sequencing determination by a base addition sequencing scheme
DE4141178A1 (de) 1991-12-13 1993-06-17 Europ Lab Molekularbiolog Verfahren zur sequenzierung von nukleinsaeuren
GB9208733D0 (en) 1992-04-22 1992-06-10 Medical Res Council Dna sequencing method
GB9210176D0 (en) 1992-05-12 1992-06-24 Cemu Bioteknik Ab Chemical method
US5516664A (en) 1992-12-23 1996-05-14 Hyman; Edward D. Enzymatic synthesis of repeat regions of oligonucleotides
US5436143A (en) 1992-12-23 1995-07-25 Hyman; Edward D. Method for enzymatic synthesis of oligonucleotides
US6074823A (en) 1993-03-19 2000-06-13 Sequenom, Inc. DNA sequencing by mass spectrometry via exonuclease degradation
FR2703052B1 (fr) 1993-03-26 1995-06-02 Pasteur Institut Nouvelle méthode de séquençage d'acides nucléiques.
GB9315847D0 (en) 1993-07-30 1993-09-15 Isis Innovation Tag reagent and assay method
US5547859A (en) 1993-08-02 1996-08-20 Goodman; Myron F. Chain-terminating nucleotides for DNA sequencing methods
DE69426731T2 (de) 1993-11-17 2001-06-28 Amersham Pharmacia Biotech Uk Ltd., Little Chalfont Verfahren zur massenspektroskopischen sequenzanalyse einer nukleinsäure mittels primerverlängerung
US5763594A (en) 1994-09-02 1998-06-09 Andrew C. Hiatt 3' protected nucleotides for enzyme catalyzed template-independent creation of phosphodiester bonds
US5872244A (en) 1994-09-02 1999-02-16 Andrew C. Hiatt 3' protected nucleotides for enzyme catalyzed template-independent creation of phosphodiester bonds
US5808045A (en) 1994-09-02 1998-09-15 Andrew C. Hiatt Compositions for enzyme catalyzed template-independent creation of phosphodiester bonds using protected nucleotides
US6214987B1 (en) 1994-09-02 2001-04-10 Andrew C. Hiatt Compositions for enzyme catalyzed template-independent formation of phosphodiester bonds using protected nucleotides
US6232465B1 (en) 1994-09-02 2001-05-15 Andrew C. Hiatt Compositions for enzyme catalyzed template-independent creation of phosphodiester bonds using protected nucleotides
CA2202480A1 (en) * 1994-10-14 1996-04-25 Jane Charlotte Moores Porphyrin labeling of polynucleotides
SE9500342D0 (sv) 1995-01-31 1995-01-31 Marek Kwiatkowski Novel chain terminators, the use thereof for nucleic acid sequencing and synthesis and a method of their preparation
AU5171696A (en) 1995-02-27 1996-09-18 Ely Michael Rabani Device, compounds, algorithms, and methods of molecular characterization and manipulation with molecular parallelism
EP0745686A1 (en) 1995-06-01 1996-12-04 Roche Diagnostics GmbH The use of DNA polymerase 3'-intrinsic editing activity
US6312893B1 (en) 1996-01-23 2001-11-06 Qiagen Genomics, Inc. Methods and compositions for determining the sequence of nucleic acid molecules
EP0992511B1 (en) 1996-01-23 2009-03-11 Operon Biotechnologies, Inc. Methods and compositions for determining the sequence of nucleic acid molecules
US6613508B1 (en) 1996-01-23 2003-09-02 Qiagen Genomics, Inc. Methods and compositions for analyzing nucleic acid molecules utilizing sizing techniques
US6005125A (en) 1996-05-06 1999-12-21 Rutgers The State University Of New Jersey Universal allyl linker for solid-phase nucleic acid synthesis
US5821356A (en) 1996-08-12 1998-10-13 The Perkin Elmer Corporation Propargylethoxyamino nucleotides
US5885775A (en) 1996-10-04 1999-03-23 Perseptive Biosystems, Inc. Methods for determining sequences information in polynucleotides using mass spectrometry
CA2277545A1 (en) 1997-01-08 1998-07-16 Proligo Llc Bioconjugation of oligonucleotides
US6046005A (en) 1997-01-15 2000-04-04 Incyte Pharmaceuticals, Inc. Nucleic acid sequencing with solid phase capturable terminators comprising a cleavable linking group
WO1998033939A1 (fr) * 1997-01-31 1998-08-06 Hitachi, Ltd. Procede pour determiner une sequence de base d'acide nucleique et appareil correspondant
BR9812270A (pt) 1997-07-28 2000-07-18 Medical Biosystems Ltd Análise de sequência de ácido nuclêico
US6008379A (en) * 1997-10-01 1999-12-28 The Perkin-Elmer Corporation Aromatic-substituted xanthene dyes
US6485944B1 (en) 1997-10-10 2002-11-26 President And Fellows Of Harvard College Replica amplification of nucleic acid arrays
GB9815163D0 (en) 1998-07-13 1998-09-09 Brax Genomics Ltd Compounds
CA2330673C (en) 1998-05-01 2009-05-26 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and dna molecules
US6780591B2 (en) 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6096875A (en) 1998-05-29 2000-08-01 The Perlein-Elmer Corporation Nucleotide compounds including a rigid linker
US6218530B1 (en) 1998-06-02 2001-04-17 Ambergen Inc. Compounds and methods for detecting biomolecules
US6287821B1 (en) 1998-06-11 2001-09-11 Orchid Biosciences, Inc. Nucleotide analogues with 3'-pro-fluorescent fluorophores in nucleic acid sequence analysis
US6218118B1 (en) 1998-07-09 2001-04-17 Agilent Technologies, Inc. Method and mixture reagents for analyzing the nucleotide sequence of nucleic acids by mass spectrometry
JP2002521064A (ja) 1998-07-30 2002-07-16 ソレックサ リミテッド アレイ生体分子およびシークエンシングにおけるその使用
GB0002310D0 (en) 2000-02-01 2000-03-22 Solexa Ltd Polynucleotide sequencing
US6787308B2 (en) 1998-07-30 2004-09-07 Solexa Ltd. Arrayed biomolecules and their use in sequencing
DE19844931C1 (de) 1998-09-30 2000-06-15 Stefan Seeger Verfahren zur DNS- oder RNS-Sequenzierung
US6451525B1 (en) 1998-12-03 2002-09-17 Pe Corporation (Ny) Parallel sequencing method
US6380378B1 (en) * 1998-12-24 2002-04-30 Toagosei Company, Ltd. Nucleotide compound, nucleotide block oligonucleotide, and method for producing them
EP2177627B1 (en) 1999-02-23 2012-05-02 Caliper Life Sciences, Inc. Manipulation of microparticles in microfluidic systems
WO2000053805A1 (en) 1999-03-10 2000-09-14 Asm Scientific, Inc. A method for direct nucleic acid sequencing
US7037654B2 (en) * 1999-04-30 2006-05-02 Aclara Biosciences, Inc. Methods and compositions for enhancing detection in determinations employing cleavable electrophoretic tag reagents
US7056661B2 (en) 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
US6818395B1 (en) 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
US6242193B1 (en) 1999-07-30 2001-06-05 Hitachi, Ltd. Apparatus for determining base sequence of nucleic acid
US6982146B1 (en) 1999-08-30 2006-01-03 The United States Of America As Represented By The Department Of Health And Human Services High speed parallel molecular nucleic acid sequencing
AU7086800A (en) 1999-08-30 2001-03-26 Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, The High speed parallel molecular nucleic acid sequencing
AU7537200A (en) 1999-09-29 2001-04-30 Solexa Ltd. Polynucleotide sequencing
US6309836B1 (en) 1999-10-05 2001-10-30 Marek Kwiatkowski Compounds for protecting hydroxyls and methods for their use
AU1471001A (en) 1999-11-04 2001-05-14 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
WO2001048184A2 (de) 1999-12-23 2001-07-05 Axaron Bioscience Ag Verfahren zur parallelen sequenzierung eines nukleinsäuregemisches an einer oberfläche
GB0002389D0 (en) 2000-02-02 2000-03-22 Solexa Ltd Molecular arrays
WO2001073121A1 (fr) 2000-03-30 2001-10-04 Toyota Jidosha Kabushiki Kaisha Procede pour determiner une sequence de base d'une molecule individuelle d'acide nucleique
GB0013276D0 (en) * 2000-06-01 2000-07-26 Amersham Pharm Biotech Uk Ltd Nucleotide analogues
GB0016473D0 (en) 2000-07-05 2000-08-23 Amersham Pharm Biotech Uk Ltd Sequencing method
CA2421582A1 (en) 2000-09-11 2002-03-21 The Trustees Of Columbia University In The City Of New York Combinatorial fluorescence energy transfer tags and uses thereof
DE10045592A1 (de) 2000-09-15 2002-03-28 Klaus Pfizenmaier Ein Antikörper-TNF-TNF Inhibitor Fusionsprotein (TNF-Selektokin) als zielspezifisches Prozytokin zur Tumortherapie
EP1337541B1 (en) 2000-10-06 2007-03-07 The Trustees of Columbia University in the City of New York Massive parallel method for decoding DNA and RNA
US7297518B2 (en) 2001-03-12 2007-11-20 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences by asynchronous base extension
US20030027140A1 (en) 2001-03-30 2003-02-06 Jingyue Ju High-fidelity DNA sequencing using solid phase capturable dideoxynucleotides and mass spectrometry
DE10120797B4 (de) 2001-04-27 2005-12-22 Genovoxx Gmbh Verfahren zur Analyse von Nukleinsäureketten
DE10120798B4 (de) 2001-04-27 2005-12-29 Genovoxx Gmbh Verfahren zur Bestimmung der Genexpression
US6613523B2 (en) 2001-06-29 2003-09-02 Agilent Technologies, Inc. Method of DNA sequencing using cleavable tags
US7057031B2 (en) 2001-07-13 2006-06-06 Ambergen, Inc. Nucleotide compositions comprising photocleavable markers and methods of preparation thereof
DE10239504A1 (de) 2001-08-29 2003-04-24 Genovoxx Gmbh Verfahren zur Analyse von Nukleinsäurekettensequenzen und der Genexpression
GB0128526D0 (en) 2001-11-29 2002-01-23 Amersham Pharm Biotech Uk Ltd Nucleotide analogues
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
DE60301622T2 (de) 2002-04-04 2006-06-14 Biotage Ab Uppsala Auf primer verlängerung basierendes verfahren, das nukleotide verwendet, die über spaltbare linker markiert sind
US20040014096A1 (en) * 2002-04-12 2004-01-22 Stratagene Dual-labeled nucleotides
US7074597B2 (en) 2002-07-12 2006-07-11 The Trustees Of Columbia University In The City Of New York Multiplex genotyping using solid phase capturable dideoxynucleotides and mass spectrometry
AU2003259352A1 (en) 2002-08-23 2004-03-11 Solexa Limited Labelled nucleotides
US7414116B2 (en) * 2002-08-23 2008-08-19 Illumina Cambridge Limited Labelled nucleotides
WO2004018497A2 (en) 2002-08-23 2004-03-04 Solexa Limited Modified nucleotides for polynucleotide sequencing
GB0321306D0 (en) 2003-09-11 2003-10-15 Solexa Ltd Modified polymerases for improved incorporation of nucleotide analogues
US7067031B2 (en) * 2003-12-03 2006-06-27 Dew Engineering And Development Limited Process for making a ceramic armor plate
US7622279B2 (en) 2004-03-03 2009-11-24 The Trustees Of Columbia University In The City Of New York Photocleavable fluorescent nucleotides for DNA sequencing on chip constructed by site-specific coupling chemistry
CA3104322C (en) 2011-09-23 2023-06-13 Illumina, Inc. Methods and compositions for nucleic acid sequencing

Also Published As

Publication number Publication date
US7785796B2 (en) 2010-08-31
SI3266791T1 (sl) 2021-04-30
EP3266791B1 (en) 2021-01-27
US20060188901A1 (en) 2006-08-24
EP2338893A1 (en) 2011-06-29
WO2003048387A3 (en) 2003-10-16
HK1248710A1 (en) 2018-10-19
EP1451351A2 (en) 2004-09-01
EP1451351B1 (en) 2017-02-01
US7057026B2 (en) 2006-06-06
KR20050044668A (ko) 2005-05-12
PT3266791T (pt) 2021-03-03
US20060160075A1 (en) 2006-07-20
US20030104437A1 (en) 2003-06-05
ES2620131T3 (es) 2017-06-27
BR0214683A (pt) 2004-11-23
DK3266791T3 (da) 2021-03-01
EP3858845A1 (en) 2021-08-04
US20100028885A1 (en) 2010-02-04
ES2858359T3 (es) 2021-09-30
AU2002349155A1 (en) 2003-06-17
JP2005511058A (ja) 2005-04-28
CN1617937A (zh) 2005-05-18
DK1451351T3 (en) 2017-03-27
CY1118765T1 (el) 2017-07-12
EP2338893B1 (en) 2017-06-28
US7427673B2 (en) 2008-09-23
US7566537B2 (en) 2009-07-28
PT1451351T (pt) 2017-03-29
IL162170A0 (en) 2005-11-20
EP2338893B9 (en) 2019-12-25
CA2469066A1 (en) 2003-06-12
IS7291A (is) 2004-06-01
WO2003048387A2 (en) 2003-06-12
EP3266791A1 (en) 2018-01-10

Similar Documents

Publication Publication Date Title
CY1123882T1 (el) Επισημασμενα νουκλεοτιδια
CY1123971T1 (el) Επισημασμενα νουκλεοτιδια
BR9807742A (pt) EpotilÈneos que são modificados na cadeia lateral
DE60332774D1 (de) Verbesserte sso7-polymerase-konjugat-proteine
DE50105789D1 (de) Acrylathaftklebemassen mit enger molekulargewichtsverteilung
ATE284407T1 (de) Pyrimidinderivate als markierte bindungspartner
DE60119416D1 (de) Grundierungszusammensetzung für druckempfindliche Klebstoffe auf Basis von Silikonen
DE69827335D1 (de) Härtbare Zusammensetzung
DE60127074D1 (de) Härtbare Siloxanklebstoffzusammensetzung
DE60100640D1 (de) Härtbare Zusammensetzung
DE60111989D1 (de) Härtbare Silikonklebstoffzusammensetzung
ES2164622T1 (es) Disposicion de portador y etiqueta.
KR980001315U (ko) 포켓용 할인쿠폰북
NO20030847L (no) Skiltanordning for hestevogn
UA3962S (uk) Рекламний буклет
UA4173S (uk) Рекламний буклет
BRPI0403880A (pt) Sistemas e métodos de ligação de materiais
BR7701495U (pt) Rótulo promocional adesivo destacável
FIU20010464U0 (fi) Opaste- tai mainostaulu
UA4538S (uk) Комплект етикеток
ES1045645Y (es) Banderola publicitaria mixta
KR980001304U (ko) 스티커 앨범
DK199800487U3 (da) Selvklæbende kuponetiket
UA2446S (uk) Жилет для розміщення рекламної інформації
BR7901917U (pt) Marcador de livro