CN203860262U - Simple aphid feeding device - Google Patents
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- CN203860262U CN203860262U CN201420246535.2U CN201420246535U CN203860262U CN 203860262 U CN203860262 U CN 203860262U CN 201420246535 U CN201420246535 U CN 201420246535U CN 203860262 U CN203860262 U CN 203860262U
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- 241001124076 Aphididae Species 0.000 title claims abstract description 23
- 229920001817 Agar Polymers 0.000 claims abstract description 17
- 239000008272 agar Substances 0.000 claims abstract description 17
- 235000013405 beer Nutrition 0.000 claims abstract description 6
- 238000009423 ventilation Methods 0.000 claims abstract description 4
- 238000009395 breeding Methods 0.000 claims description 13
- 230000001488 breeding effect Effects 0.000 claims description 13
- 241000748095 Hymenopappus filifolius Species 0.000 claims 1
- 238000001746 injection moulding Methods 0.000 claims 1
- 230000000384 rearing effect Effects 0.000 abstract description 12
- 238000012360 testing method Methods 0.000 abstract description 5
- 230000004962 physiological condition Effects 0.000 abstract description 4
- 238000005520 cutting process Methods 0.000 abstract description 3
- 238000004519 manufacturing process Methods 0.000 abstract description 2
- 239000000463 material Substances 0.000 abstract description 2
- 241000238631 Hexapoda Species 0.000 description 13
- 238000000034 method Methods 0.000 description 7
- 238000002474 experimental method Methods 0.000 description 5
- 241000894006 Bacteria Species 0.000 description 3
- 238000012136 culture method Methods 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 2
- 241000233866 Fungi Species 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 241000208125 Nicotiana Species 0.000 description 1
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 1
- 241000125167 Rhopalosiphum padi Species 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004166 bioassay Methods 0.000 description 1
- 230000009193 crawling Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
- 238000007711 solidification Methods 0.000 description 1
- 230000008023 solidification Effects 0.000 description 1
- 241000894007 species Species 0.000 description 1
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Abstract
本实用新型公开了一种蚜虫简易饲养装置,包括盒型饲养容器和与之匹配的盒盖;饲养容器的底部盛有煮沸溶解并冷却的琼脂,琼脂上贴有一层叶圆片,盒盖扎有数十个透气用的针孔;饲养装置还包括一个用来剪裁叶圆片用的叶圆片裁剪器,叶圆片裁剪器是将啤酒易拉罐的上部分切割成一面没有底的圆柱形桶;使用本饲养装置时,将供试蚜虫用细毛笔挑于叶圆片上,饲养容器扣上盒盖后将盒子反扣以满足蚜虫在叶片背面取食的自然生理条件。本实用新型结构简单、制作方便、省工省时、就地取材、经济节约、占空间少,并且可以循环使用。
The utility model discloses a simple feeding device for aphids, which comprises a box-shaped feeding container and a matching box cover; the bottom of the feeding container contains boiled, dissolved and cooled agar, and a layer of leaf discs is pasted on the agar, and the box cover is tied There are dozens of pinholes for ventilation; the rearing device also includes a leaf disc cutter for cutting leaf discs. The leaf disc cutter cuts the upper part of the beer can into a cylindrical barrel without a bottom When using this rearing device, the aphids for testing are picked on the leaf discs with a fine brush, and the rearing container is buckled on the lid and the box is reversed to meet the natural physiological conditions that the aphids feed on the back of the blade. The utility model has the advantages of simple structure, convenient manufacture, labor-saving and time-saving, local materials, economical savings, less space occupation, and can be recycled.
Description
技术领域 technical field
本实用新型涉及一种昆虫饲养装置,具体涉及一种蚜虫简易饲养装置。 The utility model relates to an insect feeding device, in particular to a simple feeding device for aphids. the
背景技术 Background technique
在昆虫科学研究中有一项基础而重要的工作就是饲养供试昆虫,饲养昆虫的作用主要是为了观察昆虫的生活史及其习性或者为生物测定提供试虫等。不同昆虫根据其不同习性,需要不同的饲养方式,即使同种昆虫,也会由于饲养目的不同需要不同饲养方式,很多科学研究由于供试昆虫饲养方法不恰当而对随后的实验造成麻烦。传统的昆虫饲养用具有养虫箱、养虫笼等,但根据昆虫种类、习性和饲养目的,都需要不同程度的改良。 One of the basic and important tasks in insect science research is to raise test insects. The function of raising insects is mainly to observe the life history and habits of insects or to provide test insects for bioassays. Different insects require different feeding methods according to their different habits. Even the same species of insects will require different feeding methods due to different feeding purposes. Many scientific studies have caused troubles for subsequent experiments due to inappropriate feeding methods for tested insects. Traditional insect breeding facilities include insect cages, insect cages, etc., but according to the insect species, habits and breeding purposes, they all need to be improved to varying degrees. the
目前,常见的蚜虫饲养装置和方法主要有微虫笼、单株饲养、叶子圆片饲养法等:微虫笼需要夹在叶片上,对于麦蚜虫比较合适,但是烟蚜饲养时因烟叶较大,很难操作;单株饲养时蚜虫活动范围广,对于蚜虫计数产生很大麻烦,而且如果每种处理的蚜虫数量较少时需要较多株苗,浪费资源并难以达到实现目的;以上方法由于所需植株较多,难操作,占空间大等原因很难大量用于试验,而叶子圆片培养法克服了以上缺点而被广泛应用。 At present, common aphid rearing devices and methods mainly include micro-worm cages, single-plant rearing, and leaf disc rearing methods, etc.: micro-worm cages need to be clamped on the leaves, which is more suitable for wheat aphids, but tobacco leaves are larger when raising aphids. , it is difficult to operate; aphids have a wide range of activities when raising a single plant, which causes great trouble for aphid counting, and if the number of aphids for each treatment is small, more seedlings are needed, which wastes resources and is difficult to achieve the goal; the above methods are due to Many plants are required, it is difficult to operate, and it takes up a lot of space. It is difficult to use them in large quantities for experiments. However, the leaf disc culture method overcomes the above shortcomings and is widely used. the
但是在已报道的叶子圆片培养法中,培养基通常使用液体培养基,这样蚜虫不可避免的会被淹死;虽然有时候也使用固体培养基,但是这些固体培养基中含有各种有利于细菌或真菌滋生的成分,即使加了抗生素,也很容易因为叶片外表面或边缘带菌而滋生细菌或真菌。因此,叶子圆片培养法对于专业技术要求比较高,在科研试验中不易于大量推广使用。 However, in the reported leaf disc culture method, the medium usually uses a liquid medium, so that the aphids will inevitably be drowned; although sometimes a solid medium is used, but these solid medium contain various beneficial Bacteria or fungi can grow, even if antibiotics are added, it is easy to breed bacteria or fungi because of bacteria on the outer surface or edge of the leaves. Therefore, the leaf disc culture method requires relatively high professional skills, and it is not easy to be widely used in scientific research experiments. the
发明内容 Contents of the invention
本实用新型所要解决的技术问题是针对现有技术中存在的不足,而提供一种蚜虫简易饲养装置,该装置结构简单、制作方便、省工省时、就地取材、经济节约、占空间少,并且可以循环使用。 The technical problem to be solved by the utility model is to provide a simple feeding device for aphids in view of the deficiencies in the prior art. , and can be recycled. the
为了实现上述目的,本实用新型是通过下述技术方案实现的: In order to achieve the above object, the utility model is achieved through the following technical solutions:
一种蚜虫简易饲养装置,包括盒型饲养容器,其特征在于,饲养容器具有一个与之匹配的盒盖;所述的饲养容器的底部盛有煮沸溶解并冷却的琼脂,琼脂上贴有一层叶圆片;所述的盒盖扎有数十个透气用的针孔。 A simple breeding device for aphids, comprising a box-shaped feeding container, characterized in that the feeding container has a matching lid; the bottom of the feeding container is filled with boiled, dissolved and cooled agar, and a layer of leaf is pasted on the agar. Disc; the lid of the box is pierced with dozens of pinholes for ventilation. the
上述的饲养装置还包括一个用来剪裁叶圆片用的叶圆片裁剪器,叶圆片裁剪器是将啤酒易拉罐的上部分切割成一面没有底的圆柱形桶;叶圆片裁剪器的规格为高15mm×底径62mm。 The above-mentioned feeding device also includes a leaf disc cutter for cutting the leaf disc, the leaf disc cutter is to cut the upper part of the beer can into a cylindrical barrel without a bottom on one side; the specification of the leaf disc cutter It is 15mm high x 62mm bottom diameter. the
上述饲养容器的规格为上口径75mm×高40mm×底径57mm,盒盖的规格为长75mm×宽40mm;饲养容器和盒盖为PP注塑制品。 The specifications of the above-mentioned breeding container are upper diameter 75mm×height 40mm×bottom diameter 57mm, and the specification of the box cover is length 75mm×width 40mm; the breeding container and box cover are PP injection-molded products. the
上述的琼脂浓度为1%,饲养容器中盛有25-35ml琼脂。 The above-mentioned agar concentration is 1%, and 25-35ml of agar is contained in the rearing container. the
上述的针孔通过用火烧过的细大头针扎成。 The above-mentioned pinholes are made by burning thin pins. the
使用本饲养装置时,将供试蚜虫用细毛笔挑于叶圆片上,饲养容器扣上盒盖后将盒子反扣以满足蚜虫在叶片背面取食的自然生理条件。 When using this rearing device, the aphids for testing are picked on the leaf discs with a fine brush, and the rearing container is buckled on the lid and the box is reversed to meet the natural physiological conditions that the aphids feed on the back of the leaves. the
本实用新型饲养装置,饲养容器和盒盖咬合很紧,密闭性很好,可以有效地防止蚜虫爬出,对生命表等实验中蚜虫计数极为重要;此外为了利于蚜虫呼吸,盒盖上具有数十个针孔;叶圆片裁剪器是将啤酒易拉罐上部分切割出直径为62mm的圆柱,这样可以利用其直接切割出直径为62mm的叶圆片;盒子反扣以满足蚜虫在叶片背面取食的自然生理条件;同种处理的重复可以叠在一起,占空间少,可以在培养箱内小空间多处理试验;本实用新型的饲养容器可以循环使用,甚至琼脂还可再融再利用,经济节约。 The feeding device of the utility model, the feeding container and the box cover are tightly occluded, and the airtightness is very good, which can effectively prevent aphids from crawling out, and is extremely important for counting aphids in experiments such as life tables; in addition, in order to facilitate aphid breathing, the box cover has a number Ten pinholes; the leaf disc cutter cuts a cylinder with a diameter of 62mm from the upper part of the beer can, so that it can be used to directly cut a leaf disc with a diameter of 62mm; the box is reversed to allow aphids to feed on the back of the leaf natural physiological conditions; the repetition of the same treatment can be stacked together, taking up less space, and multiple treatment experiments can be performed in a small space in the incubator; the breeding container of the utility model can be recycled, and even the agar can be melted and reused, which is economical save. the
附图说明 Description of drawings
图1:本实用新型的结构示意图; Fig. 1: structural representation of the utility model;
图2:本实用新型的顶盖俯视图; Figure 2: Top view of the utility model;
图3:本实用新型的饲养容器的剖视图; Fig. 3: the sectional view of the rearing container of the present utility model;
图4:本实用新型的叶圆片裁剪器的示意图; Fig. 4: the schematic diagram of the leaf disc cutter of the present utility model;
图5:本实用新型的叶圆片裁剪器的示意图; Fig. 5: the schematic diagram of the leaf disc cutter of the present utility model;
其中:1、饲养容器,2、盒盖,3、琼脂,4、叶圆片,5、针孔,6、叶圆片裁剪器,7、啤酒易拉罐。 Among them: 1. Breeding container, 2. Box cover, 3. Agar, 4. Leaf disc, 5. Pinhole, 6. Leaf disc cutter, 7. Beer can. the
具体实施方式 Detailed ways
下面结合附图对本实用新型装置进行详细的描述。 The device of the present invention will be described in detail below in conjunction with the accompanying drawings. the
如图1、图2和图3所示,一种蚜虫简易饲养装置,包括盒型饲养容器1,饲养容器1的规格优选为上口径75mm×高40mm×底径57mm;饲养容器1具有一个与之匹配的盒盖2,盒盖2的规格优选为长75mm×宽40mm;饲养容器1和盒盖2均为PP注塑制品。饲养容器1的底部盛有煮沸溶解并冷却的琼脂3,琼脂3的浓度为1%,体积为25-35ml;琼脂3上贴有一层叶圆片4,盒盖2上用火烧过的细大头针扎有数十个透气用的针孔5。 As shown in Fig. 1, Fig. 2 and Fig. 3, a kind of aphid simple raising device comprises box type raising container 1, and the specification of raising container 1 is preferably upper diameter 75mm * high 40mm * bottom diameter 57mm; The matching box cover 2, the specification of the box cover 2 is preferably 75mm in length x 40mm in width; both the breeding container 1 and the box cover 2 are PP injection-molded products. The bottom of the rearing container 1 is filled with boiled, dissolved and cooled agar 3, the concentration of the agar 3 is 1%, and the volume is 25-35ml; a layer of leaf disc 4 is attached on the agar 3, and a thin pin burned with fire is placed on the lid 2 Dozens of pinholes 5 for ventilation are pricked. the
如图4和图5所示,饲养装置还包括一个用来剪裁叶圆片4用的叶圆片裁剪器6,叶圆片裁剪器6是将啤酒易拉罐7的上部分切割成一面没有底的圆柱形桶;叶圆片裁剪器6的规格优选为高15mm×底径62mm。 As shown in Figures 4 and 5, the breeding device also includes a leaf disc cutter 6 for cutting the leaf disc 4, and the leaf disc cutter 6 is to cut the upper part of the beer pop can 7 into one side without the bottom. Cylindrical barrel; the specification of leaf disc cutter 6 is preferably high 15mm * bottom diameter 62mm. the
使用本饲养装置时: When using this feeding device:
(1)将1%的琼脂3煮沸溶解,并将溶解的琼脂3倒入饲养容器1中,每盒倒入30ml; (1) Boil and dissolve 1% agar 3, and pour the dissolved agar 3 into the breeding container 1, and pour 30ml into each box;
(2)用叶圆片裁剪器6切割出直径为62mm的叶圆片4,在琼脂3冷却至快凝固时将叶圆片4正面贴在琼脂3上,保证其没有气泡,这样叶圆片4在琼脂3凝固后便可紧贴保湿; (2) cut out the leaf disc 4 that diameter is 62mm with leaf disc cutter 6, when agar 3 is cooled to fast solidification, the front of leaf disc 4 is pasted on the agar 3, guarantees that it does not have air bubbles, so that the leaf disc 4 After the agar 3 is solidified, it can be closely attached and moisturized;
(3)将供试蚜虫用细毛笔挑于饲养容器1的叶圆片4上,盖上盒盖2,将盒子反扣以满足蚜虫在叶片背面取食的自然生理条件; (3) Pick the aphids for testing on the leaf disc 4 of the rearing container 1 with a fine brush, cover the lid 2, and buckle the box upside down to meet the natural physiological conditions that the aphids feed on the back of the blade;
(4)五天甚至更长时间更换一次,保证寄主新鲜。 (4) Replace every five days or even longer to ensure the freshness of the host. the
本实用新型结构简单、制作方便、省工省时、就地取材、经济节约、占空间少,并且可以循环使用。 The utility model has the advantages of simple structure, convenient manufacture, labor-saving and time-saving, local materials, economical savings, less space occupation, and can be recycled. the
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Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104839098A (en) * | 2015-05-26 | 2015-08-19 | 中国农业科学院植物保护研究所 | Device and method for bioassay of piercing-sucking pests |
CN106942162A (en) * | 2017-04-25 | 2017-07-14 | 遵义医学院 | A kind of device and its application method suitable for white backed planthopper feeding method RNA interference experiments |
CN107085094A (en) * | 2017-06-15 | 2017-08-22 | 华侨大学 | A bioassay method for indoor aphidicidal protein against aphids |
CN108142379A (en) * | 2017-12-12 | 2018-06-12 | 河北省农林科学院昌黎果树研究所 | For differentiating and observing the feeder of bacterium feeding habits and the small mite class of phytophagous |
-
2014
- 2014-05-14 CN CN201420246535.2U patent/CN203860262U/en not_active Expired - Fee Related
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104839098A (en) * | 2015-05-26 | 2015-08-19 | 中国农业科学院植物保护研究所 | Device and method for bioassay of piercing-sucking pests |
CN106942162A (en) * | 2017-04-25 | 2017-07-14 | 遵义医学院 | A kind of device and its application method suitable for white backed planthopper feeding method RNA interference experiments |
CN107085094A (en) * | 2017-06-15 | 2017-08-22 | 华侨大学 | A bioassay method for indoor aphidicidal protein against aphids |
CN108142379A (en) * | 2017-12-12 | 2018-06-12 | 河北省农林科学院昌黎果树研究所 | For differentiating and observing the feeder of bacterium feeding habits and the small mite class of phytophagous |
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