CN103444636B - Method for feeding tea lesser leafhoppers indoors by utilizing living tea tree leaf blades - Google Patents

Abstract

The invention discloses a method for raising tea leafhoppers indoors by using leaves of living tea trees. The tea leafhoppers to be raised are confined on the leaves of living tea trees with self-made feeding tubes to prevent the tenderness behavior of tea leaves. The tea leafhopper is forced to suck the sap from the leaf veins to obtain the nutrients needed for growth and development. The method of the invention is simple and easy, the survival rate of the small green leafhoppers raised is significantly improved, the workload is greatly reduced, and the healthy growth of tea trees for nutrition is protected, and the loss of tea trees for feeding is largely saved.

Description

A kind of method of using living tea tree leaves to raise tea little green leafhopper indoors

technical field

The invention belongs to the technical field of biological control of insects, and in particular relates to a method for raising insects indoors.

Background technique

Tea green leafhoppers, Hemiptera, Cicadellidae (Cicadellidae), commonly known as floating dust, leaf collembola, etc., occur widely, and occur in all tea-producing provinces and autonomous regions across the country. Adults and nymphs of the green leafhopper gnaw and suck the sap of tea tree shoots, consuming the nutrients and water of tea leaves; the females lay eggs in the tissues of young shoots, hindering the growth of buds, and have become the most important pests in tea gardens in my country. This insect has 8-12 generations a year, and there is a generation alternation phenomenon, which seriously damages summer and autumn tea. The buds and leaves of the affected tea trees are curled up, hardened, the tips and margins of the leaves are reddish brown and scorched, and the buds grow slowly, which seriously affects the yield and quality of tea.

According to the existing literature, most of the insect sources required for the research on the tea leafhopper are directly collected from tea gardens. The length of the wings of the green leafhopper is only 3.1-3.8mm. In addition, after the third instar, it is agile and good at jumping quickly. It is not only time-consuming and labor-intensive to capture a large number of living organisms outdoors, but also cannot guarantee the quality and stability of the test insects. At present, according to the difference of food sources, the methods of raising tea leafhoppers indoors mainly include in vitro plant breeding and live plant breeding. If you use a finger tube with fresh tea buds or leaves to raise isolated plants, this method requires frequent replacement of leaf buds or leaves, and the workload is large, and the survival rate and quality of small green leafhoppers are generally not high; Seedlings are raised and potted tea trees are raised. These two methods easily cause tea seedlings and tea trees to be seriously damaged and die, and the cost is higher and it takes more time to cultivate tea seedlings and tea trees. Generally speaking, there is still a lack of a method that can stably and efficiently obtain the experimental insects of the small green leafhopper.

Contents of the invention

In order to solve the problems of time-consuming, laborious, low survival rate and insufficient food source in the existing technology of raising green leafhoppers indoors, the present invention provides a method for raising green leafhoppers indoors, and designs a method suitable for the method. Raising observation tube, its technical scheme is as follows:

A method of using living tea tree leaves to raise tea leafhoppers indoors is characterized in that: the tea leafhoppers to be reared are restricted on the leaves of living tea trees with self-made feeding tubes to prevent the tea leafhoppers from tending to tenderness, and the tea Small green leafhoppers are forced to suck the sap in the leaf veins to obtain the nutrients needed for growth and development.

The specific steps are:

(1) Choose healthy and clean leaves on the potted tea tree, and stick the self-made feeding tube on the leaves along the main veins of the leaves. When operating, the side with the semicircular hole at the bottom faces down, so that the main veins of the leaves are stuck on the semicircular holes , to ensure that the main vein is free from mechanical damage caused by the feeding tube;

(2) Use a plastic clip to fix the feeding tube on the leaves to avoid damage to the leaves, and minimize the gap between the feeding tube and the leaves to prevent the insects to be reared from escaping;

(3) Select 1-5 small green leafhoppers that need to be reared and put them into the mouth of the pipe, cover the mouth of the pipe with a 100-mesh circular gauze the size of the mouth of the pipe, and cover the pipe.

Wherein, the living tea tree is a potted healthy growing tea tree, which can be of any variety; the leaves of the living tea tree can be new leaves or old leaves.

The tea trees are selected from varieties with large and thick tea leaves that are more than two years old, with fast growth and many buds, such as Fuyun No. 6 and Narcissus tea. Cultivate in flower pots and place them in a well-lit glass greenhouse. Timely fertilization and pruning make the tea tree grow quickly and keep the leaves clean, intact and healthy. The temperature in the glass greenhouse is 26-29°C, the relative humidity is 60-80%, and the natural photoperiod.

Wherein, the main part of the feeding tube is a transparent polypropylene tube with a round bottom and a cover with a diameter of 1-2 cm and a length of 4-10 cm. Cut the bottom of the polypropylene tube in half to the section 0.5 cm from the mouth of the bottle. A semicircle with a diameter of 1mm was punched in the center of the bottom of half of them, and the center of the tube cover was hollowed out. Each feeding tube was equipped with a plastic clip for fixing; the size of the tube used was selected according to the age and number of insects raised.

The advantages of the present invention are:

(1) The main body of the feeding tube used is a transparent plastic tube, which is convenient for observing the small green leafhoppers reared in the tube;

(2) Live plants are used for breeding, which is convenient for observation and ensures the nutrition needed for the growth of small green leafhoppers;

(3) Feed leaves can provide the nutrients needed for the growth of small green leafhoppers for a long time, saving the workload of frequent food replacement;

(4) The damage range of the small green leafhopper is limited, and the excessive damage of the host tea tree is avoided.

(5) The leaves of the living tea tree can continuously supply the nutrients needed for the growth of tea leafhoppers to be reared. The leaves will not die and fall after being damaged, and can still be used for feeding the next batch of insects after a few days of recovery.

Description of drawings

Figure 1 is a schematic diagram of the front of the feeding tube: 1 - a transparent polypropylene tube with a round bottom and a cover, 2 - blades.

Figure 2 is a schematic diagram of the bottom of the feeding tube: 3-the semicircular hole at the bottom of the tube.

Figure 3 is a schematic diagram of the mouth of the feeding tube: 4-circular gauze (100 mesh), 5-the center of the hollowed-out tube cover.

Figure 4 is a schematic diagram of the use of feeding tubes: 1-transparent polypropylene tube with round bottom and lid, 6-leaf main vein, 7-plastic clip.

Fig. 5 is the physical picture of tea little green leafhopper breeding

Detailed ways

The following are specific embodiments of the present invention, which further illustrate the present invention, but the present invention is not limited thereto.

Embodiment 1: The adaptability of small green leafhoppers to feed on leaves of different ages

Take a transparent polypropylene tube 1 with a round bottom and a cap with a diameter of 2 cm and a length of 10 cm (Figure 1), cut the section from the bottom of the polypropylene tube to 0.5 cm from the mouth of the bottle in half, and punch a hole in the center of the bottom half of the tube A semicircle 3 with a diameter of 1mm (Fig. 2); the center 5 of the tube cover (Fig. 3) is hollowed out, and a circular gauze (100 mesh) 4 (Fig. 3) with the size of a hole is cut out for use, and the feeding tube is made according to this method 27 Each tube is equipped with a plastic clip 7 (Figure 4).

Choose three pots of Narcissus tea trees, and select 9 healthy leaves 2 on each pot of tea trees (Figure 1). The distribution of these 9 leaves is: 3 leaves on new shoots, 3 leaves on one-year branches, and 3 leaves on old branches. Attach the prepared feeding tube to the blade along the main vein 6 (Figure 4). During operation, the side with the semicircular hole at the bottom faces down, so that the main vein of the blade is stuck on the semicircular hole to ensure that the main vein is not affected by the feeding tube. caused mechanical damage. Afterwards, the feeding tube is fixed on the blade with a plastic clip, and the gap between the feeding tube and the blade can be reduced at the same time to prevent the worms to be raised from escaping. Pick a total of 27 small green leafhopper nymphs from 3 to 4 instars, put one into each tube, cover the mouth of the tube with a piece of gauze, and tightly cover the tube cover. The physical picture of feeding is shown in Figure 5.

The results showed that more than 90% of the small green leafhopper nymphs reared on these three types of leaves could emerge normally, and there was no significant difference.

Embodiment 2: the individual rearing of tea little green leafhopper nymph

Take a transparent polypropylene tube 1 with a round bottom and a cap with a diameter of 1 cm and a length of 4 cm, cut the section from the bottom of the polypropylene tube to 0.5 cm from the mouth of the bottle in half, and make a semicircle with a diameter of 1 mm in the center of the bottom half 3; the center 5 of the tube cover is hollowed out, and a circular gauze 4 (100 orders) of the size of a tube mouth is cut for subsequent use, and 50 feeding tubes are made in this way, and each tube is equipped with a plastic clip 7.

Choose five potted Narcissus tea trees, choose 9 pieces of healthy leaves 2 on each tea tree (3 pieces of new shoots, 3 pieces of one-year branches, and 3 pieces of old branches), and insert the feeding tube along the main vein of the leaves On the blade, the side with the semicircular hole at the bottom faces down during operation, so that the main vein of the blade is stuck on the semicircular hole to ensure that the main vein is free from mechanical damage caused by the feeding tube. Afterwards, the feeding tube is fixed on the blade with a plastic clip, and the gap between the feeding tube and the blade can be reduced at the same time to prevent the worms to be raised from escaping. Put one first instar nymph into each tube afterwards, cover the mouth of the tube with a piece of gauze, and cover the tube cover tightly. The survival and molting of each nymph were recorded daily.

The results show that more than 90% of the nymphs on freshly drawn young leaves can successfully survive to eclosion, and there is no need to replace the leaves during the period from nymphs to eclosion. The eclosion rate of leafhoppers in one year is slightly lower, less than 80%. The eclosion rate is the lowest, about 30%, and most nymphs die at the first and second instars. The reason may be that the mouthparts of the first instar nymphs are too soft to pierce the too hard leaf veins, resulting in starvation to death. After the small green leafhopper emerges, the tube is removed from the blade, and the blade does not wither or fall off, and can continue to grow. The adult body length (average male 2.29mm, female 2.66mm, excluding wing length) is smaller than that of adults grown in tea gardens (average male 2.35mm, female 2.83mm, excluding wing length), but the difference is not significant. Adults after eclosion replace blades and continue to raise with this feeding tube, and can survive for a long time.

Claims (2)

1. a method of utilizing live body tea leaf indoor feeding tea lesser leafhopper, it is characterized in that: tea lesser leafhopper to be raised is limited on the blade of live body tea tree with homemade raising pipe, stop the tender behavior that becomes of tea lesser leafhopper, the juice that tea lesser leafhopper is forced through in thorn phyllidium arteries and veins obtains the required nutrition of growing, and concrete steps are as follows:

(1) on potted plant tea tree, select healthy, clean blade, homemade raising pipe is stuck on blade along blade master pulse, bottom during operation, there is a side of semicircle orifice down, blade master pulse is stuck on semicircle orifice, to guarantee that master pulse avoids being subject to raising the mechanical damage of pipe racks; Wherein, the blade of live body tea tree is young leaves or Lao Ye;

(2) with a plastic grip, raising pipe is fixed on blade, avoids damaged blade, and reduce to raise pipe and interlobate gap as far as possible, prevent from waiting supporting insect and run away;

(3) choose and need the smaller green leaf hopper 1-5 head of raising to put into from the mouth of pipe, mouth of pipe place covers the circular gauze of 100 orders of a slice mouth of pipe size, lid upper tube cap.

2. the method for utilizing live body tea leaf indoor feeding tea lesser leafhopper according to claim 1, it is characterized in that: the main part of described raising pipe is diameter 1-2cm, the round bottom of long 4-10cm even covers transparent polypropylene pipe, polypropylene tube bottom to this section from bottleneck 0.5cm is half-and-half cut, the semicircle of a diameter 1mm is made a call to by the bottom centre of half therein, Guan Gai center hollows out, and each raises Guan Peiyi plastic grip for fixing; The size of pipe used is selected according to the length of time and the quantity of supporting worm.