CN1387034A - Method and device for quickly measuring photosynthesizing speed of delay light generated by visiblelight inducing - Google Patents
Method and device for quickly measuring photosynthesizing speed of delay light generated by visiblelight inducing Download PDFInfo
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- CN1387034A CN1387034A CN 02115395 CN02115395A CN1387034A CN 1387034 A CN1387034 A CN 1387034A CN 02115395 CN02115395 CN 02115395 CN 02115395 A CN02115395 A CN 02115395A CN 1387034 A CN1387034 A CN 1387034A
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- delayed luminescence
- light
- photosynthetic rate
- visible light
- induced
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- 238000000034 method Methods 0.000 title claims abstract description 17
- 230000001939 inductive effect Effects 0.000 title description 13
- 238000003996 delayed luminescence Methods 0.000 claims abstract description 48
- 230000000243 photosynthetic effect Effects 0.000 claims abstract description 42
- 230000005284 excitation Effects 0.000 claims abstract description 24
- 238000001514 detection method Methods 0.000 claims abstract description 15
- 241000196324 Embryophyta Species 0.000 claims abstract description 9
- 210000003763 chloroplast Anatomy 0.000 claims abstract description 6
- 230000003111 delayed effect Effects 0.000 claims abstract 4
- 230000029553 photosynthesis Effects 0.000 claims description 4
- 238000010672 photosynthesis Methods 0.000 claims description 4
- 230000006378 damage Effects 0.000 claims description 2
- 230000004300 dark adaptation Effects 0.000 claims 1
- 239000000463 material Substances 0.000 claims 1
- 239000002184 metal Substances 0.000 claims 1
- 230000003595 spectral effect Effects 0.000 claims 1
- 238000001228 spectrum Methods 0.000 claims 1
- 210000001519 tissue Anatomy 0.000 claims 1
- 238000001429 visible spectrum Methods 0.000 claims 1
- 230000007613 environmental effect Effects 0.000 abstract description 2
- 230000006698 induction Effects 0.000 abstract 1
- 238000005259 measurement Methods 0.000 description 7
- 239000013307 optical fiber Substances 0.000 description 6
- 235000017060 Arachis glabrata Nutrition 0.000 description 3
- 241001553178 Arachis glabrata Species 0.000 description 3
- 235000010777 Arachis hypogaea Nutrition 0.000 description 3
- 235000018262 Arachis monticola Nutrition 0.000 description 3
- 101000694017 Homo sapiens Sodium channel protein type 5 subunit alpha Proteins 0.000 description 3
- 238000003306 harvesting Methods 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 235000020232 peanut Nutrition 0.000 description 3
- 229930002868 chlorophyll a Natural products 0.000 description 2
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 241000628997 Flos Species 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000003384 imaging method Methods 0.000 description 1
- 239000000411 inducer Substances 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 230000002045 lasting effect Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 230000004962 physiological condition Effects 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
- 230000005068 transpiration Effects 0.000 description 1
- WFKWXMTUELFFGS-UHFFFAOYSA-N tungsten Chemical compound [W] WFKWXMTUELFFGS-UHFFFAOYSA-N 0.000 description 1
- 229910052721 tungsten Inorganic materials 0.000 description 1
- 239000010937 tungsten Substances 0.000 description 1
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- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
本发明提供一种利用可见光诱导的叶绿体延迟发光,进行光合速率快速检测的方法和装置,包括下述步骤:植物叶片在可见光的诱导下产生延迟发光;光接受组件将此发光信号转换为电信号;电信号通过模数转换为数字信号;处理数字信号得到叶片的光诱导的延迟发光强度;在已建立的延迟发光强度与光合速率相对应的数据库中对比此发光强度,得出其光合速率。一种可见光诱导的延迟发光光合快速检测装置包括如下组件:光激发组件、光接受组件、模数转换器、计算机和数据处理软件。本发明不需要破坏叶片,受外界环境因素的影响小,操作简便。
The invention provides a method and device for rapid detection of photosynthetic rate by utilizing delayed luminescence of chloroplasts induced by visible light, comprising the following steps: plant leaves produce delayed luminescence under the induction of visible light; light-receiving components convert the luminescent signal into an electrical signal ; the electrical signal is converted into a digital signal through analog-to-digital; the digital signal is processed to obtain the light-induced delayed luminous intensity of the leaves; the luminous intensity is compared in the established database corresponding to the delayed luminous intensity and the photosynthetic rate, and the photosynthetic rate is obtained. A visible light-induced delayed luminescence photosynthetic rapid detection device comprises the following components: a light excitation component, a light receiving component, an analog-to-digital converter, a computer and data processing software. The invention does not need to destroy blades, is less affected by external environmental factors, and is easy to operate.
Description
(1) technical field
The present invention relates to a kind of photosynthesis rate measuring technique, method and the device thereof that photosynthetic rate detects carried out in particularly a kind of delayed luminescence that utilizes visible light-inducing.
(2) background technology
In photosynthesis rate was measured, generally the instrument of Shi Yonging was a LI-62XX series photosynthetic rate analyzer at present.Its principle is according to blade CO on unit interval, unit area
2Reduction determine the photosynthetic rate of blade.There are many problems in this instrument, mainly shows the following aspects: (1) this instrument in measuring process is subjected to the restriction of external environment factor more serious.As light intensity, humidity, CO
2The change of concentration moment all can bring very big difference to measurement result.Therefore, measure one group of active data and often will spend long time.(2) because when measuring, the lasting time is longer, and be engraved in change during extraneous ambient condition, therefore, the data that record are a series of random numbers, can not reflect the photosynthetic rate of tested plant under physiological condition of living at that time.(3) design limit of leaf chamber to the measurement of blade.Because the leaf chamber size is fixing, the blade blade face of measurement is excessive or too smallly all can make troubles to measurement, even brings error to measurement result.(4) since in measuring process the breathing and the transpiration of plant leaf blade, make the environmental baseline (CO that leaf is indoor
2Concentration, humidity etc.) and extraneous condition inconsistent, and instrument itself can not well compensate this difference.(5) this instrument cost is relatively more expensive, is unfavorable for the universal use in agricultural production.
(3) summary of the invention
The object of the present invention is to provide a kind of delayed luminescence to carry out the photosynthetic rate method for quick by visible light-inducing.Mainly be to use the harmless visible light of blade is carried out exciting of short time to blade, induce chloroplast to produce the delayed luminescence of long period, and utilize this delayed luminescence to characterize the photosynthetic rate of blade.
Another object of the present invention is to provide a kind of delayed luminescence photosynthetic rate device for fast detecting of realizing the visible light-inducing of said method.
Purpose of the present invention is achieved through the following technical solutions, and the delayed luminescence photosynthetic rate detection method of this visible light-inducing comprises the steps:
(1) blade is placed the darkroom, dark adatpation 10～20s;
(2) be that 2750～5500lux, wavelength coverage are the even irradiation blade of excited by visible light light source 0.2～20s of 400～760nm with light intensity, the inducer blade chloroplast produces the delayed luminescence of long period;
(3) utilize optical fiber receive module to receive this delayed luminescence signal, and convert thereof into electric signal from blade;
(4) electric signal is converted to digital signal by analog to digital converter;
(5) utilize software that digital signal is carried out data processing, obtain blade integral or local delayed luminescence intensity.
(6) in delayed luminescence intensity of having set up and the corresponding database of photosynthetic rate, contrast this luminous intensity, obtain the photosynthetic rate of tested blade.
The wavelength of selected exciting light sources can be chosen in the wavelength of visible light scope, and the exciting light light intensity is lower than the blade injury threshold value.
Close light source after inducing chloroplast to produce the delayed luminescence of long period with the even irradiation blade of excitation source, darkening 2～10s can avoid the interference from the fluorescence of chlorophyll a like this; Because of delayed luminescence decay fast in time, shorten the darkening time, can improve the delayed luminescence signal intensity.
Above-mentioned excitation source is a light intensity when being the excitation source of 2750～5500lux, and evenly irradiation blade 0.2～20s can obtain long-term delayed luminescence signal.
The delayed luminescence photosynthetic rate pick-up unit of visible light-inducing of the present invention comprises that electrical connection between electrical connection between excited by visible light assembly, optical fiber receive module, digital to analog converter, computing machine, light excitation component and the computing machine, optical fiber receive module and the digital to analog converter, analog to digital converter are connected with signal between the computing machine.
Described optical fiber receive module is the device that is used to survey light, and is comparatively faint comparatively speaking because of photoinduction delayed luminescence, can adopt the weak light detection system, for example various photomultipliers, CCD or enhancement mode CCD imaging system (ICCD) etc.
Described analog to digital converter can adopt general analog to digital converter, or has other devices of analog-digital conversion function.The CCD controller that for example image series capture card of National Instruments company production, or Princeton Instruments company is produced.
General data-signal process software is housed in the described computing machine,, is used for blade delayed luminescence Signal Processing as the LabVIEW software of NationalInstruments company exploitation or the WINVIEW software of Princeton Instruments company exploitation.
The present invention compared with prior art has following advantage and effect:
(1) the delayed luminescence photosynthetic rate method for quick of this visible light-inducing combines the short time irradiation delayed luminescence of visible light and the advantage of optical imagery (when receiving unit is CCD or ICCD), the photosynthetic power of blade can be described from morphologic angle, the average photosynthesis rate that not only can reflect whole blade, and can also reflect the power of blade different parts photosynthetic rate, compare with traditional detection method and have more directly perceived, clear comprehensive advantage.
(2) the delayed luminescence photosynthetic rate fast detecting of this visible light-inducing is to carry out in dark situation (leaf chamber).Utilize the excitation source that carries when exciting, can fixedly excite light intensity to every group of measurement, avoided in the traditional technique in measuring process changing the error of the measurement data of bringing owing to the moment of light intensity, therefore, stable, accurate, timesaving advantage that this method has.
(3) the delayed luminescence photosynthetic rate detection method operating process of this visible light-inducing is simple, realizes easily; And the apparatus structure of realizing this method is simple, and the production assembling is more or less freely, easy for operation, and the package unit cost is relatively low, is beneficial to popularize and promote.
(4) description of drawings
Fig. 1 is a kind of device synoptic diagram of the delayed luminescence photosynthetic rate device for fast detecting of visible light-inducing of the present invention;
Fig. 2 is that peanut leaf dark adatpation 20s excites darkening 2s in the 4s darkroom to open the take pictures shutter image of camera lens of light harvesting by computer control then in concrete the enforcement;
Fig. 3 is applied to big leaf of Flos Carthami sheet photosynthetic rate detected image with above-mentioned steps.
(5) embodiment
Below in conjunction with accompanying drawing the present invention is done further concrete description, but embodiments of the present invention are not limited thereto.
Execute example 1
Fig. 1 has provided a kind of concrete structure synoptic diagram of the delayed luminescence photosynthetic rate device for fast detecting of visible light-inducing of the present invention.As seen from Figure 1, this device comprises excitation component, optical fiber receive module, analog to digital converter, computing machine, printer.Wherein optical fiber receive module is connected and composed by take pictures camera lens and the detector ICCD that collect light; Light excitation component and computing machine are electrically connected, and reception group, analog to digital converter and computing machine are electrically connected successively; Three-dimensional adjustable specimen holder is placed blade to be measured on the frame, place the darkroom.Select for use each member to connect to form this device, wherein, it is visible excitation source that excitation source is selected wavelength coverage for use, and this selects the 50W tungsten lamp for use.The camera lens of taking pictures is selected the big numerical aperture camera lens (50mm, f/1.2 type) of the Nikon company manufacturing that is applicable to that the low light level is taken for use; The ICCD-576-S/1 (enhancement mode CCD) that detector selects for use U.S. Princeton Instruments company to make; The ST-130 type controller that analog to digital converter selects for use U.S. PrincetonInstruments company to make; Computing machine is selected the Pentium III type microcomputer of Intel Company for use; Data processing software is selected WINVIEW for use.
Said apparatus is applied to the detection of the photosynthetic rate of peanut: peanut leaf is placed on the sample stage in darkroom, make its place, focal plane that is in the light harvesting camera lens, close the darkroom, dark adatpation 20s.By the shutter that is in the light of computer control excitation source, make exciting light evenly excite the blade face.Excite the be in the light shutter and the power supply of closing excitation source behind the 4s by computer control, fluorescence for fear of chlorophyll a, close being in the light soon behind the door of excitation source, allow blade darkening 2s in the darkroom, open the take pictures shutter of camera lens of light harvesting by computer control then, the delayed luminescence of record blade is carried out image weight by analog to digital converter and computing machine, obtain the image of delayed luminescence, as shown in Figure 2.The delayed luminescence of record blade is carried out image by analog to digital converter and computing machine and is weighed the delayed luminescence image that obtains.With WINVIEW software processes image, obtain blade integral or local delayed luminescence intensity, in delayed luminescence intensity of having set up and the corresponding database of photosynthetic rate, contrast this luminous intensity, obtain corresponding to the photosynthetic rate of this luminous intensity.The average photosynthetic rate of its correspondence is 5.4ppm/scm
2With WINVIEW software processes image, obtain blade integral delayed luminescence intensity, in delayed luminescence intensity of having set up and the corresponding database of photosynthetic rate, contrast this luminous intensity, obtain corresponding to the photosynthetic rate of this luminous intensity.The average photosynthetic rate of its correspondence is 5.4ppm/scm
2
Embodiment two
According to the aforesaid operations step, measure the photosynthetic rate of plant bright red flower.The image of rebuilding as shown in Figure 3, its corresponding average photosynthetic rate is 3.6ppm/scm
2
Claims (10)
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB021153957A CN1168973C (en) | 2002-06-18 | 2002-06-18 | Photosynthetic rate measurement method and device for visible light-induced delayed luminescence |
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| CNB021153957A CN1168973C (en) | 2002-06-18 | 2002-06-18 | Photosynthetic rate measurement method and device for visible light-induced delayed luminescence |
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| CN1387034A true CN1387034A (en) | 2002-12-25 |
| CN1168973C CN1168973C (en) | 2004-09-29 |
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| CNB021153957A Expired - Fee Related CN1168973C (en) | 2002-06-18 | 2002-06-18 | Photosynthetic rate measurement method and device for visible light-induced delayed luminescence |
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100397069C (en) * | 2004-04-28 | 2008-06-25 | 华南师范大学 | Monitoring method and device for ecological environment and environmental pollution |
| CN100447568C (en) * | 2005-04-19 | 2008-12-31 | 华南师范大学 | Light-emitting diode irradiation photosynthetic ability rapid detector |
| CN1804589B (en) * | 2005-12-28 | 2010-05-12 | 华南师范大学 | A method and device for plant stress physiological monitoring and high-resistance plant screening |
| CN1804590B (en) * | 2005-12-28 | 2010-05-12 | 华南师范大学 | A monitoring method for crop growth and development regulation |
| CN103234916A (en) * | 2013-04-06 | 2013-08-07 | 吉林大学 | Prediction method for net photosynthetic rate of population |
| CN103925992A (en) * | 2013-01-16 | 2014-07-16 | 光宝电子(广州)有限公司 | Brightness measurement method and system with backlight device |
| CN103954562A (en) * | 2014-04-09 | 2014-07-30 | 福建农林大学 | Photosynthesis machine based on optical fiber sensor |
| CN104713861A (en) * | 2015-03-17 | 2015-06-17 | 深圳市海王英特龙生物技术股份有限公司 | Dark cavity and in-vitro detection and analysis device using dark cavity |
| CN105891162A (en) * | 2016-03-30 | 2016-08-24 | 山东省花生研究所 | Method for measuring peanut photosynthetic lagged extent by constructing mathematical model |
| CN106525821A (en) * | 2016-10-31 | 2017-03-22 | 山东省医药生物技术研究中心 | Method and device for detecting medicinal property of traditional Chinese medicine |
| CN110398485A (en) * | 2018-04-24 | 2019-11-01 | 南京简智仪器设备有限公司 | A detection device and detection method based on differential Raman technology |
-
2002
- 2002-06-18 CN CNB021153957A patent/CN1168973C/en not_active Expired - Fee Related
Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100397069C (en) * | 2004-04-28 | 2008-06-25 | 华南师范大学 | Monitoring method and device for ecological environment and environmental pollution |
| CN100447568C (en) * | 2005-04-19 | 2008-12-31 | 华南师范大学 | Light-emitting diode irradiation photosynthetic ability rapid detector |
| CN1804589B (en) * | 2005-12-28 | 2010-05-12 | 华南师范大学 | A method and device for plant stress physiological monitoring and high-resistance plant screening |
| CN1804590B (en) * | 2005-12-28 | 2010-05-12 | 华南师范大学 | A monitoring method for crop growth and development regulation |
| CN103925992B (en) * | 2013-01-16 | 2016-03-16 | 光宝电子(广州)有限公司 | There are the brightness measurement method and system of the device of backlight |
| CN103925992A (en) * | 2013-01-16 | 2014-07-16 | 光宝电子(广州)有限公司 | Brightness measurement method and system with backlight device |
| CN103234916B (en) * | 2013-04-06 | 2015-03-18 | 吉林大学 | Prediction method for net photosynthetic rate of population |
| CN103234916A (en) * | 2013-04-06 | 2013-08-07 | 吉林大学 | Prediction method for net photosynthetic rate of population |
| CN103954562A (en) * | 2014-04-09 | 2014-07-30 | 福建农林大学 | Photosynthesis machine based on optical fiber sensor |
| CN104713861A (en) * | 2015-03-17 | 2015-06-17 | 深圳市海王英特龙生物技术股份有限公司 | Dark cavity and in-vitro detection and analysis device using dark cavity |
| CN105891162A (en) * | 2016-03-30 | 2016-08-24 | 山东省花生研究所 | Method for measuring peanut photosynthetic lagged extent by constructing mathematical model |
| CN105891162B (en) * | 2016-03-30 | 2018-07-03 | 山东省花生研究所 | A kind of method for measuring the photosynthetic delay degree of peanut by building mathematical model |
| CN106525821A (en) * | 2016-10-31 | 2017-03-22 | 山东省医药生物技术研究中心 | Method and device for detecting medicinal property of traditional Chinese medicine |
| CN106525821B (en) * | 2016-10-31 | 2019-03-22 | 山东省医药生物技术研究中心 | A kind of method and device detecting herbal nature |
| CN110398485A (en) * | 2018-04-24 | 2019-11-01 | 南京简智仪器设备有限公司 | A detection device and detection method based on differential Raman technology |
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| Publication number | Publication date |
|---|---|
| CN1168973C (en) | 2004-09-29 |
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