CN102608042A - Device and method for extrinsic allergen quantitative detection based on multispectral imaging - Google Patents

Abstract

The invention proposes an in vitro allergen quantitative detection device and method based on multispectral imaging, which are used in allergen detection technology. The device includes a light source circuit, a light intensity correction module, a multi-spectral spectroscopic module, an optical signal capture module and a storage table. The spectroscopic modules are placed directly above the storage table together. In the method, the light source provided by the light source circuit is calibrated, the multi-spectral spectroscopic module used is provided with filters covering the wavelength range of visible light, the reagent strips to be detected are photographed under each filter, and the images are processed to obtain the detection results. The method of the invention improves the accuracy of quantitative detection, reduces costs, and can realize large-throughput automatic detection, saving manpower.

Description

An in vitro allergen quantitative detection device and method based on multispectral imaging

technical field

The invention belongs to the technical field of biology and medicine, relates to an allergen detection method and device, in particular to an in vitro allergen quantitative detection device and method based on multispectral imaging.

Background technique

The incidence of allergic diseases in the world is increasing day by day, and the conditions are becoming more and more complex. Allergens are the cause of allergic inflammation, so detecting and discovering allergens and taking effective measures to avoid contact with them are of great significance for the prevention and treatment of allergic diseases. Currently, the most important methods for detecting allergens include in vivo and in vitro tests. In vivo tests are expensive, are greatly affected by individual differences, and may cause serious consequences. The in vitro test is safe and reliable, and is suitable for patients with severe skin diseases, extreme sensitivity to allergens, or who cannot stop taking drugs that interfere with the skin test. In vitro allergen detection has a good application prospect, and has developed from the initial qualitative detection and semi-quantitative detection to the current quantitative detection. Quantitative detection method has become the development direction of in vitro allergen detection due to its automation, high efficiency and quickness, and accurate results. The most important in vitro test is the detection of IgE (immunoglobulin E, immunoglobulin E). IgE antibodies exist in the serum of individuals with allergic constitution, so in vitro detection of serum allergen-specific IgE (specific IgE, referred to as sIgE) and total IgE (total IgE, referred to as tIgE) levels can be used to determine the sensitivity of the body to allergens .

Enzyme-linked immunosorbent assay (ELISA) is currently the main in vitro allergen detection method. By coating the surface of the reagent strip with a single allergen, a mixture of allergens or a monoclonal antibody, the slgE antibody in the patient's serum reacts with them separately, forms an immune complex and then precipitates. Then add the enzyme and substrate, and the reagent strip after the final reaction will show different shades of color. The quantitative result of the degree of allergy can be obtained by detecting the color depth of the reagent strip.

Accurate judgment of the device is crucial for grading the degree of allergy. Existing technologies, such as the Rapid detector of German MEDIWISS company and the IVT detector of American ASI company, use white light to irradiate the reagent strip, and use color detectors to obtain the RGB three components of the reflected signal. However, there is some overlap between the different color components. It will lead to limitations in obtaining the original information, thereby limiting the improvement of the quantitative detection ability of allergens.

Contents of the invention

Aiming at the limitation of obtaining color information of detection reagent strips in the prior art, which limits the accuracy of allergen quantitative detection, the present invention provides an in vitro allergen quantitative detection device and method based on multispectral imaging.

The present invention proposes an in vitro allergen quantitative detection device based on multi-spectral imaging. The detection device includes a light source circuit, a light intensity correction module, a multi-spectral spectroscopic module, an optical signal capture module and a storage platform. The storage platform is a water platform. The reagent strip to be detected is placed on the storage table, and the multi-spectral light-splitting module and the optical signal capture module are arranged directly above the storage table, and the multi-spectral light-splitting module is arranged below the optical signal capture module; the light source circuit is located at the top left of the storage table, and To provide the light source required for the test, emit white light obliquely onto the storage table; the light intensity correction module is fixed directly below the light source circuit (1), monitors the intensity of the light source in real time, converts the collected optical signal into a digital signal, and converts the collected optical signal into a digital signal. The digital signal is transmitted to the computer to correct the error caused by the shaking of the light source.

Described multi-spectral spectroscopic module comprises optical filter, optical filter wheel, central axis, rotary table and motion controller; Optical filter wheel is the wheel disc that rotates with central axis, and is evenly opened on the circumference of wheel disc near the edge. There is a row of holes, and a filter is fixed at each hole; the filter wheel is fixed with the rotary table through the central shaft; the rotary table is connected to the motion controller through a serial port transmission line; the computer controls the motion controller to advance or retreat in a specific step Long, it drives the rotating table to rotate, and the rotating table drives the filter wheel to rotate coaxially, so that a certain filter is rotated to the right below the lens of the optical signal capture module, and the filter is closely combined with the lens.

The in vitro allergen quantitative detection method based on multispectral imaging proposed by the present invention specifically includes the following steps:

Step 1, initializing the test device, includes three steps:

Step 11, turn on the light source circuit, the light intensity correction module, the multi-spectral spectroscopic module and the optical signal capture module, preheat the planar LED white light source of the light source circuit for 1 minute, after preheating, the planar LED white light source emits white light;

Step 1.2, through the computer, control the motion controller to advance to a specific step length, drive the rotary table to rotate, and the filter wheel fixed on the rotary table will rotate at a specific angle coaxially, and the filter of a certain wavelength will be rotated to Directly below the lens and closely combined with the lens;

Step 1.3, adjust the exposure time of the CCD image collector and take pictures, so that the maximum gray value of the captured image is between 200-230.

Step 2, get the picture: use the lens to take pictures, and at the same time of taking pictures every time, the light intensity correction module collects the optical signal of the white light emitted by the light source circuit, converts the optical signal into a digital signal, displays and records it on the computer ; Correct the error caused by light source shaking in the computer: set the light source light intensity value A obtained before taking the first photo as the standard, and then record the current light source light intensity value B at the same time of taking pictures each time, and use the difference (A-B) Correct the gray value of the picture taken this time.

Step 3, turn the filter wheel until the next filter is facing the lens, repeat steps 1.2 to 1.3 and step 2 until all 11 filters have been used, then proceed to step 4.

Step 4, process the image data stored in the computer through an image processing program, first obtain the gray value of the image of the reagent strip to be detected, then obtain the eigenvalue of the image of the reagent strip to be detected, and then according to the eigenvalue-IgE concentration standard The curve is used to obtain the IgE concentration of the reagent strip to be detected, and finally to obtain the quantitative detection result of the allergen.

Advantage and positive effect of the present invention are:

(1) An in vitro allergen quantitative detection device and method based on multispectral imaging proposed by the present invention uses a CCD camera and multiple wavelength filters to collect the intensity information of the optical signal, and collects the optical signal synchronously within the light source irradiation range. The fluctuation information of the signal can obtain the fluctuation information of the light source in a short imaging time, so as to correct the gray information of the image of the reagent strip to be detected, and improve the accuracy of quantitative detection;

(2) An in vitro allergen quantitative detection device and method based on multi-spectral imaging proposed by the present invention can correct the error caused by uneven light source irradiation through a standard color card with uniform chromaticity, which is conducive to improving the accuracy of quantitative detection ;

(3) A kind of in vitro allergen quantitative detection device and method based on multi-spectral imaging proposed by the present invention uses a plurality of filters of different wavelengths to filter light intensity information, and can obtain more wavelength-light source information feature points, which has It is beneficial to comprehensively analyze the images of the reagent strips to be detected.

Description of drawings

Fig. 1 is the structural representation of the in vitro allergen quantitative detection device of the present invention;

2 is a schematic diagram of the composition of the light source circuit in the in vitro allergen quantitative detection device of the present invention;

3 is a schematic diagram of the composition of the light intensity correction module in the in vitro allergen quantitative detection device of the present invention;

4 is a schematic diagram of the composition of the multi-spectral spectroscopic module in the in vitro allergen quantitative detection device of the present invention;

Fig. 5 is a schematic top view of the filter wheel in the in vitro allergen quantitative detection device of the present invention:

Fig. 6 is a schematic structural view of the optical signal capture module in the in vitro allergen quantitative detection device of the present invention;

Fig. 7 is a schematic flowchart of the steps of the in vitro allergen quantitative detection method of the present invention.

in:

1-Light source circuit; 2-Light intensity correction module; 3-Multi-spectral light splitting module; 4-Optical signal capture module; 5-Stock; 6-Computer; 101-LED white light source; 102-Constant current power supply; Support device; 201-optical fiber; 202-detector; 203-data collector; 301-filter; 302-filter wheel; 303-central axis; 304-rotary table; 305-motion controller; 401-CCD Image acquisition device; 402-lens; 403-CCD fixing device.

Detailed ways

The present invention will be further described in detail with reference to the accompanying drawings and embodiments.

The in vitro allergen quantitative detection system adopted in the present invention detects the allergen reagent strip prepared on the basis of enzyme-linked immunosorbent method through multi-spectral imaging technology. The spectral curve of the reagent strip is obtained by multi-spectral imaging technology, the characteristic value is obtained from the spectral curve, and the quantitative result of the allergen sIgE is obtained through the standard curve of sIgE concentration-characteristic value. In the case of high quantitative accuracy, the cost is reduced, and large-throughput automatic detection can be realized, saving manpower.

The present invention provides an in vitro allergen quantitative detection device based on multispectral imaging, as shown in FIG.

The storage table 5 is a water platform on which the reagent strips to be detected are placed. A multi-spectral light splitting module 3 and an optical signal capture module 4 are arranged directly above the storage table 5 , and the multi-spectral light splitting module 3 is installed below the optical signal capture module 4 . The light source circuit 1 is located at the top left of the storage table 5, provides a light source, emits the white light required for the test, and the white light is irradiated on the storage table 5. The light intensity correction module 2 is fixed directly below the light source circuit 1, and is used to monitor the light intensity of the light source in real time and correct errors caused by light source shaking.

As shown in FIG. 2 , the light source circuit 1 includes a planar LED white light source 101 , a constant current power supply 102 and a circuit supporting device 103 . The function of the light source circuit 1 is to emit the white light required for the test. The circuit supporting device 103 is used to fix the LED white light source 101 and the constant current power supply 102 , and the constant current power supply 102 is connected to 220V AC to provide the required constant driving current for the LED white light source 101 . The LED white light source 101 emits white light obliquely onto the reagent strip to be detected on the storage table 5 , and the included angle of the light range emitted by the LED white light source 101 is 100-120 degrees.

As shown in FIG. 3 , the light intensity correction module 2 includes an optical fiber 201 , a detector 202 and a data collector 203 . Since the planar LED white light source 101 is not a constant light source, it may cause the light source to vibrate, causing slight changes in light intensity, and changes in light intensity will affect the detection results. The function of the light intensity correction module 2 is to monitor the intensity of the white light emitted by the planar LED white light source 101 in real time, convert the collected optical signal into a digital signal and send it to the computer 6, and correct the error caused by the vibration of the light source. One end of the optical fiber 201 is placed within the illumination range of the LED white light source 101 , and the other end is connected to the detector 202 . Detector 202 is a photodiode that converts an optical signal into an electrical signal. The data collector 203 is connected to the detector 202 and connected to the computer 6 through serial communication. The data collector 203 includes a current amplifier and an A/D acquisition card. First, the LED white light source 101 emits white light, and after being transmitted through the optical fiber 201, the optical signal is converted into an electrical signal by the photodiode of the detector 202, and the electrical signal is amplified by the current amplifier of the data collector 203, and collected by an A/D acquisition card , converting the electrical signal into a digital signal, and transmitting the digital signal to the computer 6 through serial port communication.

When the optical signal capture module 4 takes a photo of the reagent strip to be detected on the storage table 5, the light intensity correction module 2 will acquire the light intensity value of the light source at the same time, and record the initial value before taking the first reagent strip image. The light source light intensity value A, based on the light source light intensity value A, obtains the light source light intensity value B at the time when the image is taken, compares the light source light intensity information B with the initial light source light intensity information A, and obtains the corresponding The difference (A-B) is added and subtracted as a correction value, and the grayscale value of the corresponding captured image is adjusted with the correction value (A-B).

As shown in FIG. 4 , the multispectral spectroscopic module 3 includes a filter 301 , a filter wheel 302 , a central shaft 303 , a rotating table 304 and a motion controller 305 . The filter wheel 302 is a roulette that rotates with the central axis 303, and is fixed together with the rotary table 304 through the central axis 303. The filter 301 is evenly fixed on the periphery of the roulette near the edge, and the rotary table 304 is connected to the motion control Device 305, motion controller 305 is connected with computer 6. The wavelength value of filter 301 is respectively 405-20nm (nanometer), 425-30nm, 450-30nm, 470-30nm, 490-30nm, 510-20nm, 530-30nm, 550-30nm, 570-20nm, 590nm -30nm, 610-20nm, a total of 11 filters, the wavelength value X-Ynm means that the filter with a wavelength of Xnm is floating up and down Y/2nm, for example, 405-20nm means that a filter with a wavelength of 405nm has Up and down 10nm float. Filters with these values cover the wavelength range of visible light.

As shown in FIG. 5 , the filter 301 is fixed on the filter wheel 302 . The filter wheel 302 is a single-row N-hole density disc, which is a wheel disc that can rotate with the central axis 303. N represents the number of holes. In the embodiment of the present invention, N is 11, and a filter is installed at each hole. 301. Every time the filter wheel 302 rotates by a specific angle, a certain filter 301 can be rotated to a specific position. In this way, the optical filter 301 with a specific wavelength value required during the experiment can be selected.

The filter wheel 302 is fixed on the rotary table 304 through the central shaft 303, and the filter wheel 302 is driven by the rotation of the rotary table 304. The rotary table 304 is connected with the motion controller 305 through a serial port transmission line, and the motion controller 305 is connected by a computer 6 Take control. The control of the motion controller 305 is realized by operating the software program on the computer 6, so that the motion controller 305 advances or retreats by a specific step, thereby driving the rotating table 304 to rotate a specific angle clockwise or counterclockwise, and then driving the optical filter The wheel 302 rotates a certain angle clockwise or counterclockwise, and the optical filter 301 of a certain wavelength is rotated directly under the lens 402, and is closely combined with the CCD lens 402 to realize batch collection of data. The collected data refers to the The image of the allergen reagent strip to be detected is captured by the optical signal capturing module 4 . After the collection of a batch of data is completed, the motion controller 305 automatically drives the filter wheel 302 to select the filter 301 of the next wavelength value.

As shown in FIG. 6 , the optical signal capture module 4 includes a CCD image acquisition 401 , a lens 402 and a CCD fixing device 403 . The CCD image acquisition 401 is connected with the lens 402 , and is buckled upside down on the CCD fixing device 403 , so that the lens 402 faces the object table 5 . The CCD image acquisition 401 converts the optical signal into a digital signal, and displays it in the form of a grayscale image in the computer 6 connected to it. The CCD image picker 401 is mechanically connected with the lens 402, and the lens 402 is stuck on the CCD image picker 401, and the CCD image picker 401 and the lens 402 are integrated for taking pictures and capturing the Image of the reagent strip to be tested. The image enters the CCD image collector 401 in the form of an optical signal, and the CCD image collector 401 converts the optical signal into a digital signal and displays it on the computer 6 as a grayscale image.

The storage table 5 is used for placing the reagent strips to be detected. The distance between the object table 5 and the CCD lens 402 is within the focal length range of the CCD lens 402 . So that the reagent strip to be detected is within the best observation range of the lens.

The aforementioned CCD image collector 401 , data collector 203 and motion controller 305 are all connected to the computer 6 . The collected data are all stored in the computer 6 and processed by relevant software programs.

The in vitro allergen quantitative detection method based on multispectral imaging proposed by the present invention specifically includes the following steps:

Step 1: Initialize the test device.

The main function of this step is to complete the initialization required for the test.

(1) Turn on the light source circuit 1 , the light intensity correction module 2 , the multispectral light splitting module 3 and the optical signal capture module 4 . And the planar LED white light source 101 is preheated for 1 minute. After preheating, the planar LED white light source 101 emits white light with stable intensity. At this time, the white light emitted from the planar LED white light source 101 is irradiated onto the reagent strip to be detected placed horizontally on the storage table 5 .

(2) The filter 301 is fixed on the filter wheel 302, and the program on the computer 6 is operated to drive the motion controller 305, so that the motion controller 305 advances to a specific step, drives the rotary table 304 to rotate, and is fixed on the rotary table The filter wheel 302 on the 304 rotates coaxially with a specific angle accordingly, and the filter 301 of a certain wavelength rotates directly under the CCD lens 402 and is closely combined with the CCD lens 402 .

(3) Adjust the exposure time of the CCD image collector 401 . The reagent strip to be detected on the object table 5 is within the focus range of the CCD image collector 401 . Using a computer image preprocessing program, adjust the exposure time of the CCD image collector 401 and take pictures, so that the maximum gray value of the captured image is between 200-230. If the grayscale value does not meet the requirements, adjust the exposure time of the CCD image collector 401 again, and repeat this step until the maximum grayscale value of the obtained image is between 200 and 230, then a suitable exposure time is found.

Step 2: Get the picture.

While taking pictures each time, the optical signal 201 located in the irradiation range of the planar LED white light source 101 transmits the optical signal emitted by the planar LED white light source 101, and the optical signal is converted into an electrical signal by the detector 202, and passed through the detector 202 phase. The connected data collector 203 converts the electrical signal into a digital signal, which is displayed and recorded on the computer 6 .

Before taking pictures, record the initial light intensity value A of the light source. After each photo is taken, the current light intensity value B of the light source is recorded and compared with the initial light intensity value of the light source to obtain the corresponding addition and subtraction difference (A-B). For the grayscale value of the picture after taking the picture, the light intensity information difference value of the light source is used for correction, so as to realize the correction of the shaking of the light source 101 . The purpose of this step is to remove the influence of light intensity changes.

Step 3: Turn the filter wheel 302 until the next filter 301 faces the CCD lens 402, repeat (2) and (3) and step 2 of step 1 until all 11 filters 301 have been used, enter Execute step 4.

Step 4: Image processing and analysis.

The picture data collected in step 2 will be saved in the computer 6 and processed by an image processing program. This step is to get specific allergen test results.

The gray value of the image of the reagent strip to be detected is obtained through the image processing program, and then the eigenvalue of the image of the reagent strip to be detected is calculated, and the IgE concentration of the reagent strip to be detected is obtained according to the eigenvalue-IgE concentration standard curve, thereby obtaining the allergen quantification Test results.

Factory setting: Illumination uniformity correction:

Since the planar LED white light source 101 is a point light source, and the light source is irradiated on the reagent strip to be detected at a certain angle during the experiment, the gray value of the image captured by the CCD image collector 401 is different at different pixel points. , affecting the experimental results. Therefore, it is necessary to correct the problem of uneven illumination when the set of detection devices leaves the factory. And in each subsequent experiment, it is necessary to perform image analysis after correction according to this standard. Specifically, a standard color card picture with uniform chroma is used for correction.

Use the gray value A of the central pixel of the standard color card image as a reference, and make a difference B-A between the gray value B of other pixels on the standard color card and this reference, and get the correction percentage (B-A)/A*100 %. Then use the obtained correction percentage to correct the pixel gray value of the image of the reagent strip to be detected.

For example, for a color card with a pixel size of 3*3, the gray value of the center pixel is a ₂₂ , and the gray values of the remaining pixels are $\left[\begin{array}{c}{a}_{11}...a_{12}...a_{13}\\ a_{\mathrm{twenty\; one}}...a_{\mathrm{twenty\; two}}...a_{\mathrm{twenty\; three}}\\ a_{31}...a_{32}...a_{33}\end{array}\right],$ Then the corrected value is $\left[\begin{array}{c}\frac{a_{11}-a_{\mathrm{twenty\; two}}}{a_{\mathrm{twenty\; two}}}...\frac{a_{12}-a_{\mathrm{twenty\; two}}}{a_{\mathrm{twenty\; two}}}...\frac{a_{13}-a_{\mathrm{twenty\; two}}}{a_{\mathrm{twenty\; two}}}\\ \frac{a_{\mathrm{twenty\; one}}-a_{\mathrm{twenty\; two}}}{a_{\mathrm{twenty\; two}}}...\frac{a_{\mathrm{twenty\; two}}-a_{\mathrm{twenty\; two}}}{a_{\mathrm{twenty\; two}}}...\frac{a_{\mathrm{twenty\; three}}-a_{\mathrm{twenty\; two}}}{a_{\mathrm{twenty\; two}}}\\ \frac{a_{31}-a_{\mathrm{twenty\; two}}}{a_{\mathrm{twenty\; two}}}...\frac{a_{32}-a_{\mathrm{twenty\; two}}}{a_{\mathrm{twenty\; two}}}...\frac{a_{33}-a_{\mathrm{twenty\; two}}}{a_{\mathrm{twenty\; two}}}\end{array}\right].$

Then, for a test reagent strip image with 3*3 pixels, the initial gray value is $\left[\begin{array}{c}{b}_{11}...b_{12}...b_{13}\\ b_{\mathrm{twenty\; one}}...b_{\mathrm{twenty\; two}}...b_{\mathrm{twenty\; three}}\\ b_{31}...b_{32}...b_{33}\end{array}\right],$ After correction is $\left[\begin{array}{c}{b}_{11}*(1-\frac{a_{11}-a_{\mathrm{twenty\; two}}}{a_{\mathrm{twenty\; two}}})...b_{11}*(1-\frac{a_{12}-a_{\mathrm{twenty\; two}}}{a_{\mathrm{twenty\; two}}})...b_{11}*(1-\frac{a_{13}-a_{\mathrm{twenty\; two}}}{a_{\mathrm{twenty\; two}}})\\ b_{11}*(1-\frac{a_{\mathrm{twenty\; one}}-a_{\mathrm{twenty\; two}}}{a_{\mathrm{twenty\; two}}})...b_{11}*(1-\frac{a_{\mathrm{twenty\; two}}-a_{\mathrm{twenty\; two}}}{a_{\mathrm{twenty\; two}}})...b_{11}*(1-\frac{a_{\mathrm{twenty\; three}}-a_{\mathrm{twenty\; two}}}{a_{\mathrm{twenty\; two}}})\\ b_{11}*(1-\frac{a_{31}-a_{\mathrm{twenty\; two}}}{a_{\mathrm{twenty\; two}}})...b_{11}*(1-\frac{a_{32}-a_{\mathrm{twenty\; two}}}{a_{\mathrm{twenty\; two}}})...b_{11}*(1-\frac{a_{33}-a_{\mathrm{twenty\; two}}}{a_{\mathrm{twenty\; two}}})\end{array}\right].$

In the image analysis of step 4 in the test, the gray value of each pixel point of each image of the reagent strip to be detected needs to be corrected with this percentage before data analysis is performed.

The invention uses a CCD camera and different wavelength filters to sample the intensity information of optical signals, and then establishes the mapping relationship between the optical signal intensity information and the concentration of allergen IgE. During the detection process, the problems of light source shaking and uneven light source irradiation are corrected, which improves the accuracy of quantitative detection. The present invention utilizes a wide-spectrum light source (wavelength covering 400nm to 700nm), and uses a plurality of filters of different wavelengths to obtain light intensity information of different spectral bands, and obtains more wavelength-light source information feature points, which is conducive to comprehensive analysis of the to-be-detected Reagent strip images for accurate allergen detection results.

During the pre-experiment, the light intensity of the LED white light source usually fluctuates within 3%. Therefore, setting the light intensity correction module (2) for real-time monitoring and correction of the grayscale information of the image of the reagent strip to be detected is beneficial to improving the accuracy of quantitative detection.

During the pre-experiment, the gray value of different areas of the standard color card has a large change. For example, the gray value of the middle pixel is 110, and the edge may be 86, 130, etc. If no correction is made, the experimental results will be affected , therefore, the modification percentage set by the present invention is very necessary.

Claims (7)

1. external anaphylactogen detection by quantitative device based on multispectral imaging; It is characterized in that; This pick-up unit comprises circuit of light sources (1), light intensity correction module (2), multispectral spectral module (3), optical signalling trapping module (4) and articles holding table (5); Articles holding table (5) is a horizontal stand; Reagent strip to be detected is placed on the articles holding table (5), is provided with multispectral spectral module (3) and optical signalling trapping module (4) directly over the articles holding table (5), and multispectral spectral module (3) is arranged on the below of optical signalling trapping module (4); Circuit of light sources (1) is positioned at the upper left side of articles holding table (5), is used to provide test required light source, and the oblique fire of emission white light line is to articles holding table (5); Light intensity correction module (2) be fixed on circuit of light sources (1) under, monitor the intensity of light source in real time, convert the optical signalling that collects into digital signal, in digital data transmission to computing machine (6), proofread and correct because the error that the light source shake causes;

Described multispectral spectral module (3) comprises optical filter (301), optical filter wheel (302), central shaft (303), universal stage (304) and motion controller (305); Optical filter wheel (302) is with the wheel disc of central shaft (303) rotation, evenly has a round at wheel disc on the submarginal week, and each place, hole is fixed with an optical filter (301); Optical filter wheel (302) is fixing with universal stage (304) through central shaft (303); Universal stage (304) connects motion controller (305) through the serial ports transmission line; Advance or retreat specific step-length through computing machine (6) controlled motion controller (305); Driven rotary platform (304) rotates; Universal stage (304) drives optical filter wheel (302) coaxial rotation; Some optical filters (301) are rotated to optical signalling trapping module (4) camera lens (402) under, and optical filter (301) and camera lens (402) are combined closely.

2. a kind of external anaphylactogen detection by quantitative device based on multispectral imaging according to claim 1 is characterized in that described circuit of light sources (1) comprises planar LED white light source (101), constant-current supply (102) and circuit supporting device (103); Circuit supporting device (103) is used for planar LED white light source (101) and constant-current supply (102) fixing; Constant-current supply (102) inserts the alternating current of 220v, for LED white light source (101) provides constant drive current; The angle of the light scope that LED white light source (101) sends is 100～120 degree.

3. a kind of external anaphylactogen detection by quantitative device based on multispectral imaging according to claim 1 is characterized in that described light intensity correction module (2) comprises optical fiber (201), detector (202) and data acquisition unit (203); One end of optical fiber (201) places in the illumination range of the white light line that circuit of light sources (1) provided, and the other end links to each other with detector (202), and optical fiber (201) sends to detector (202) with the optical signalling that monitors; Detector (202) is a photodiode, converts optical signalling into electrical signal and sends to data acquisition unit (203); Data acquisition unit (203) comprises current amplifier and A/D capture card; After the current amplifier amplification of electrical signal by data acquisition unit (203); Convert electrical signal into digital signal through the A/D capture card; Digital signal is transferred to computing machine (6) through serial ports, in computing machine (6), proofreaies and correct because the error that the light source shake causes.

4. a kind of external anaphylactogen detection by quantitative device according to claim 1 based on multispectral imaging; It is characterized in that; Described optical filter (301) has 11; The wavelength value is respectively 405-20nm, 425-30nm, 450-30nm, 470-30nm, 490-30nm, 510-20nm, 530-30nm, 550-30nm, 570-20nm, 590-30nm and 610-20nm, and X-Ynm representes that wavelength is that the optical filter of X nanometer has floating of Y/2 nanometer up and down.

5. a kind of external anaphylactogen detection by quantitative device according to claim 1 based on multispectral imaging; It is characterized in that; Described optical signalling trapping module (4) comprises ccd image collector (401), camera lens (402) and CCD stationary installation (403), and ccd image collector (401) is fixing with camera lens (402), tips upside down on the CCD stationary installation (403); Camera lens (402) is over against articles holding table (5), and the distance of articles holding table (5) and camera lens (402) is positioned at the focal range of camera lens (402); Ccd image collector (401) is connected with computing machine (6), will be digital signal through the image transitions that camera lens (402) is gathered, and the form with gray level image shows in computing machine (6).

6. an application rights requires the external anaphylactogen quantitative detecting method of 1 described external anaphylactogen detection by quantitative device based on multispectral imaging, it is characterized in that this method comprises the steps:

Step 1, the initialization test unit comprised for three steps:

Step 11; Open circuit of light sources (1), light intensity correction module (2), multispectral spectral module (3) and optical signalling trapping module (4); With planar LED white light source (101) preheating of circuit of light sources (1) 1 minute, after the preheating, planar LED white light source (101) emission white light line;

Step 12; Through computing machine (6); Controlled motion controller (305) the specific step-length of advancing, optical filter wheel (302) the specific angle of coaxial rotation thereupon on the universal stage (304) is fixed in driven rotary platform (304) rotation; The optical filter (301) of a certain wavelength is turned under the camera lens (402), and combine closely with camera lens (402);

Step 1.3, the time shutter of adjustment ccd image collector (401) also takes pictures, and the maximum gradation value that makes captured image is between 200-230;

Step 2; Obtain picture: utilize camera lens (402) to take pictures, and when taking pictures, the optical signalling of the white light line that light intensity correction module (2) collection circuit of light sources (1) is launched; at every turn And convert optical signalling into digital signal, on computing machine (6), show and note; In computing machine (6), proofread and correct because the error that the light source shake causes: will take the light source intensity value A that obtains before first photo and be made as standard; Then when taking pictures at every turn; Write down current light source intensity value B, the gray-scale value of the picture that this time clap that utilizes difference (A-B) to oppose is proofreaied and correct;

Step 3 judges whether 11 optical filters (301) all used, and rotates optical filter wheel (302) to next optical filter (301) if not over against camera lens (402), and repeating step 1.3 and step 2 are if get into step 4 and carry out;

Step 4; To being kept at the image data in the computing machine (6), handle through image processing program, at first obtain the gray-scale value of reagent strip image to be detected; Obtain the eigenwert of reagent strip image to be detected then; According to eigenwert-IgE concentration standard curve, obtain the IgE concentration of reagent strip to be detected again, finally obtain anaphylactogen detection by quantitative result.

7. a kind of external anaphylactogen quantitative detecting method according to claim 6; It is characterized in that; Described step 4 before handling image data through image processing program, needs earlier with the pixel gray-scale value of revising every reagent strip image to be detected of percent correction; Concrete grammar is: at first; As benchmark, it is poor that each the gray values of pixel points B on the standard color comparison card and benchmark A are done with standard color comparison card picture centre gray values of pixel points A, obtains the correction number percent that each pixel is corresponding on the standard color comparison card to be: (B-A)/and A*100%; Then, the pixel gray-scale value C with reagent strip image to be detected utilizes the correction number percent of pixel corresponding on the standard color comparison card to revise: C* (1-(B-A)/A*100%).

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