CN100447568C - Light-emitting diode irradiation photosynthetic ability rapid detector - Google Patents

Abstract

The present invention provides a quick detector for the photosynthetic capacity of LED irradiation, which comprises a dark room, a visual light exciting assembly, a faint light detecting assembly, an analog-digital converter and a data processing assembly, wherein the dark room has the set structure in which a living organism can extend conveniently, and the visual light exciting assembly is an LED illumination component; the weak light detecting assembly comprises optical fibre and a photomultiplier tube module, one end of the optical fibre extends into the dark room, and the other end of the optical fibre is connected with the photomultiplier tube module; the date processing assembly is a monolithic computer processing system; on one hand, the monolithic computer processing system is connected with the photomultiplier tube module through the analog-digital converter, and on the other hand, the monolithic computer processing system is also connected with the photomultiplier tube module through a control interface. The present invention has the characteristics of small volume, light weight, convenient movement and good portability. The present invention can realize the indoor and outdoor in vivo measurement on sites. The present invention overcomes the defect in the existing detection devices of errors of measurement data, which are brought by separation detection. The present invention has the advantages of accurate measurement result, simple and reasonable structure, convenient operation and low manufacture cost, and is favorable to popularization and application.

Description

Light-emitting diode irradiation photosynthetic ability rapid detector

technical field

The invention relates to a device for measuring photosynthesis rate, in particular to a rapid detector for photosynthetic ability of light-emitting diode (LED) irradiation.

Background technique

The rate of photosynthesis is an important index to characterize the photosynthetic ability of plants. At present, in the measurement of photosynthetic rate, the widely used instrument is LI-62XX series photosynthetic rate measuring instrument. The principle is to determine the photosynthetic rate of leaves according to the reduction of CO ₂ per unit time and unit area. There are many problems in this instrument, which are mainly manifested in the following aspects: (1) The instrument is seriously restricted by external environmental factors during the measurement process. For example, instantaneous changes in light intensity, humidity, and _CO2 concentration will bring great differences to the measurement results. Therefore, it often takes a long time to measure a valid set of data. (2) Since the measurement lasts for a long time, and the external environmental state is changing all the time, the measured data is a series of random numbers, which cannot reflect the photosynthetic rate of the tested plant under the physiological conditions at that time . (3) Due to the respiration and transpiration of plant leaves during the measurement process, the environmental conditions ( _CO2 concentration, humidity, etc.) in the leaf chamber are inconsistent with the external conditions, and the instrument itself cannot compensate for this difference well. (4) This instrument price is more expensive, is unfavorable for popularizing and using in agricultural production.

In order to overcome the shortcomings and deficiencies of the above-mentioned equipment, the invention patent No. ZL02115395.7 applied for by the applicant discloses a "photosynthetic rate measuring device for visible light-induced delayed luminescence", which has the advantages of low manufacturing cost, measurement The effect is good, but there are still the following problems: (1) because the darkroom of its component part is a fixed structure with a large volume, and the equipment such as a microcomputer is also a relatively large-scale equipment, so this device cannot be used according to the measurement Flexible movement is required, and the leaves of the plants must be taken off and placed in a darkroom for measurement, that is, in-vitro measurement, which will inevitably affect the accuracy of the measurement results. Since the in-vivo field measurement cannot be realized, the application range is relatively limited; ( 2) During the measurement process, due to structural problems, there is a time delay of 2 to 5 seconds when receiving delayed fluorescence, because the downward trend of delayed fluorescence is very fast, and most of the useful information is before 5 seconds, so this is extremely It greatly reduces the acquisition of useful information in delayed fluorescence, and greatly affects the correlation between delayed fluorescence and photosynthetic efficiency, which has a great impact on practicality; (3) using CCD or ICCD as the light receiving component, the volume of itself It is relatively large, and liquid nitrogen cooling is required, which increases the volume of the entire device, makes the device less portable, is not conducive to outdoor living body measurement, and is limited in use.

Contents of the invention

The object of the present invention is to overcome the shortcomings and deficiencies of the prior art, and provide a rapid detector for photosynthetic ability of light-emitting diode (LED) irradiation with reasonable structure, good portability, which can be used for on-the-spot measurement in living bodies and has good effect.

The purpose of the present invention is achieved through the following technical solutions: the LED irradiation photosynthetic ability rapid detector includes a darkroom, a visible light excitation component, a weak light detection component, an analog-to-digital converter, a data processing component, a visible light excitation component and a weak light detection component and The darkroom is connected, and the weak light detection component is connected with the data processing component through an analog-to-digital converter (A/D converter), and it is characterized in that: the darkroom is set as a structure that is convenient for the living body to extend into; the visible light excitation component is an LED Light source; described weak light detection assembly comprises optical fiber, photomultiplier tube module, and one end of optical fiber stretches into darkroom, and the other end is connected with photomultiplier tube module, and described data processing assembly is single-chip processing system, and described single-chip processing system passes through on the one hand The analog-to-digital converter is connected with the photomultiplier tube module, so that the photomultiplier tube module is sent to the single-chip processing system for processing after obtaining the signal, and on the other hand, it communicates with the photomultiplier tube module through a control interface (such as an analog switch, a relay). Connect to control the start or stop of the photomultiplier tube module.

The darkroom is an upper and lower split structure, including an upper chamber body and a lower chamber body, the upper and lower chamber bodies are connected by hinges or hinges, and the upper and lower chamber bodies are provided with mutually fitted convex rings and grooves , the matching connection between the convex ring and the groove can form a dark cavity, and a through-hole surface is also provided on the relative mating surface of the upper and lower chamber bodies. Live placement.

The upper and lower chamber bodies of the darkroom are provided with light-shielding gaskets near the through-hole surface; they are mainly used to compress the living body and block light, so that the darkroom is light-tight.

The upper and lower chamber bodies of the darkroom can also be completely separated structures, and its internal structure is the same as above; when not in use, the upper and lower chamber bodies can be separated, and when needed, the upper and lower chambers can be separated. body anastomosis.

A fixing structure (such as a bayonet, a buckle) is arranged between the upper and lower chamber bodies of the darkroom, which is convenient for fixing the upper and lower chamber bodies after docking.

A spring can also be arranged between the upper and lower chamber bodies of the darkroom; it is convenient to separate the upper and lower chamber bodies after the darkroom is used.

The darkroom can also be of an integrated structure, and a living body is provided with an insertion hole on the side of the darkroom.

A light-shielding gasket is provided near the position of the living body extending into the hole in the dark room; it can compress the living body and block the light, making the dark room light-tight.

Compared with the prior art, the present invention has the following advantages and effects: (1) because the darkroom of the LED irradiation photosynthetic ability rapid detector is designed as a structure that is small and easy to move, and the relevant electrical equipment also adopts a small structure The structural form (such as the traditional microcomputer is replaced by a single-chip microcomputer, and the larger CCD or ICCD is replaced by an optical fiber and a photomultiplier tube module, etc.), so the detector has small size, light weight, easy movement, and good portability. advantage. (2) This device can realize indoor and outdoor live body measurement, overcomes the shortcomings of measurement data errors caused by in-vitro detection of existing detection equipment, and makes the measurement results more accurate. (3) The detector is equipped with an excitation LED light source, and the intensity of the excitation light can be fixed for each group of measurements, avoiding the measurement data error caused by the instantaneous change of the light intensity in the traditional method of detection, so that the device has a stable, Accurate, time-saving advantages. (4) The device has a simple and reasonable structure, is convenient to operate, and has low cost, which is beneficial for popularization and application.

Description of drawings

Fig. 1 is a schematic structural view of a light-emitting diode irradiation photosynthetic ability rapid detector of the present invention.

Fig. 2 is a schematic structural diagram of the darkroom shown in Fig. 1 .

Fig. 3 is a block diagram of the electric connection of the light-emitting diode irradiation photosynthetic ability rapid detector of the present invention.

Fig. 4 is an electrical connection diagram of the single chip microcomputer shown in Fig. 3 and the A/D converter.

FIG. 5 is an electrical connection diagram of the single-chip microcomputer shown in FIG. 3 and the photomultiplier tube module.

Fig. 6 is an electrical connection diagram of the single chip microcomputer shown in Fig. 3 and the LED light source assembly.

Fig. 7 is an electrical connection diagram of the single chip microcomputer shown in Fig. 3 and the serial interface.

FIG. 8 is an electrical connection diagram between the A/D converter and the photomultiplier tube module shown in FIG. 3 .

Detailed ways

The present invention will be further described in detail below in conjunction with the embodiments and accompanying drawings.

Example 1

Fig. 1 has shown concrete structure of the present invention, as seen from Fig. 1, this LED irradiation photosynthetic ability rapid detector comprises darkroom 1, LED light source J7, optical fiber 3, photomultiplier tube module J4, A/D converter U6, single-chip microcomputer U1, input keyboard 5, display 4, serial interface J2, LED light source J7 is located in darkroom 1, one end of optical fiber 3 stretches in darkroom 1, and the other end is connected with photomultiplier tube module J4, and photomultiplier tube module J4 passes A/ The D-converter U6 is connected with the single-chip microcomputer U1, and the input keyboard 5 and the display 4 are connected with the single-chip microcomputer U1 at the same time. The single-chip microcomputer U1 is also connected with a serial interface J2, and can communicate with the computer through the serial interface J2. The structure of described darkroom 1 is as shown in Figure 2, as can be seen from Figure 2, described darkroom 1 is upper and lower split structure, comprises upper chamber body 1-1, lower chamber body 1-2, upper and lower chamber body 1-1, 1-2 are connected by a hinge 1-3, and the upper and lower chamber bodies 1-1, 1-2 are provided with mutually fitted protruding rings 1-4 and grooves 1-5, the protruding rings 1 -4 and groove 1-5 can be connected to form a dark cavity, and a through-hole surface 1-6 is also provided on the upper and lower chamber body 1-1, 1-2 relative mating surfaces, and the two through-hole surfaces 1-6 are opposite to each other. Cooperate can form the through hole that is communicated with the dark chamber by the outside, is convenient to the placement of living body 2; The upper and lower chamber body 1-1, 1-2 of described darkroom 1 is provided with light-shielding gasket ( not shown in the figure); mainly used to compress the living body and block the light, so that the dark chamber is light-tight; the upper and lower chamber bodies 1-1, 1-2 of the darkroom 1 are provided with a fixed bayonet 1- 7. Through the connection of the fixed bayonet 1-7, the upper and lower chambers 1-1, 1-2 can be fixed after docking; a spring is also arranged between the upper and lower chambers 1-1, 1-2 1-8, it is easier to open the upper and lower chamber bodies 1-1, 1-2 after the darkroom 1 is used.

Figures 3 to 8 show the electrical connection structure of this device. It can be seen from Figure 3 that the single-chip microcomputer U1 is electrically connected to the LED light source J7, A/D converter U6, photomultiplier tube module J4, keyboard, display J6, and interface J2 at the same time. On the one hand, the single-chip microcomputer U1 is connected with the photomultiplier tube module J4 through the A/D converter U6, so that the photomultiplier tube module J4 can be sent to the single-chip microcomputer U1 for processing after obtaining the signal; The photomultiplier tube module J4 is connected to control the start or stop of the photomultiplier tube module J4. The connection between the single-chip microcomputer U1 and the A/D converter U6 is shown in Figure 4, specifically: the single-chip microcomputer U1 controls the four control pins of the A/D converter U6 through the signal line of the P1 port (pins 2, 3, 4, and 5) (5, 13, 14, 6) Complete the collection of delayed fluorescent signals, and receive this data through the data bus (32, 33, 34, 35, 36, 37, 38, 39 pins of U1) to complete the collection of delayed fluorescent signals . The connection between the single-chip microcomputer U1 and the photomultiplier tube module J4 is shown in Figure 5, specifically: after the signal line (pin 6) of the P1 port of the single-chip microcomputer U1 passes through pins 6 and 7 of the driver chip 75451, the control relay 946H-1C-5D normally The connection of the open contact (6) means that pin 1 of the photomultiplier tube module J4 is connected with +5VA, and now the photomultiplier tube module J4 starts to work. The connection between the single-chip microcomputer U1 and the LED light source J7 is shown in Figure 6, specifically: after the P1 port signal line (pin 7) of the single-chip microcomputer U1 passes through pins 1 and 2 of the driver chip 75451, the control relay 946H-1C-5D normally open contacts The connecting of point (6) means that 1 pin of LED light source J7 is connected with +12VA, and now LED light source J7 starts to work. The connection between the single-chip microcomputer U1 and the serial interface J2 is shown in Figure 7, specifically: the P3 port signal line (10th and 11th pins) of the single-chip microcomputer U1 passes through the 9th and 10th pins of the interface chip MAX202, and connects with the 2nd pin of the serial interface J2 , 3 feet connected, so that the communication between the microcontroller and the computer can be realized. The connection between the A/D converter U6 and the photomultiplier tube module J4 is shown in Figure 8, specifically: 9, 10 of the A/D converter U6 are respectively connected to 3, 4 of the photomultiplier tube module J4 to establish Delay the transmission path of the fluorescent signal from the photomultiplier tube module J4 to the A/D converter U6, and the transmission process is realized by the control of the single-chip microcomputer U1. The optional types of each component are as follows: U1: AT89C52; U6: MAX1166; J4: MD-963; J2: RS-232.

Example 2

This embodiment is the same as embodiment 1 except for the following features: the upper and lower chamber bodies of the darkroom are completely split and separated structures, and the internal structure is the same as that of embodiment 1; when not in use, the upper and lower chamber bodies can be Separate, and connect the upper and lower chambers when needed.

Example 3

This embodiment is the same as Embodiment 1 except for the following features: the darkroom has an integrated structure, a living body insertion hole is provided on the side of the darkroom, and a light-shielding gasket is provided at a position close to the living body insertion hole in the darkroom, which can Compress the living body and block the light to make the dark room light-tight.

The above-mentioned embodiment is a preferred embodiment of the present invention, but the embodiment of the present invention is not limited by the above-mentioned embodiment, and any other changes, modifications, substitutions, combinations, Simplifications should be equivalent replacement methods, and all are included in the protection scope of the present invention.

Claims (8)

1, a kind of LED quick detector for photosynthetic capacity of irradiation, comprise darkroom, excited by visible light assembly, weak light detection assembly, analog to digital converter, data handling component, excited by visible light assembly and weak light detection assembly are connected with the darkroom, the weak light detection assembly is connected with data handling component by analog to digital converter, it is characterized in that: described darkroom is set to be convenient to the structure that live body stretches into; Described excited by visible light assembly is a led light source; Described weak light detection assembly comprises optical fiber, photomultiplier transit tube module, optical fiber one end stretches into the darkroom, the other end is connected with the photomultiplier transit tube module, described data handling component is a scm managing system, described scm managing system is connected with described photomultiplier transit tube module by analog to digital converter on the one hand, is connected with the photomultiplier transit tube module by control interface on the other hand.

2, LED quick detector for photosynthetic capacity of irradiation according to claim 1, it is characterized in that: described darkroom is upper and lower opposite opened structure, comprise chamber body, following chamber body, upper and lower chamber body is connected by hinge or hinge, upper and lower chamber body is provided with chimeric bulge loop and groove mutually, being connected of described bulge loop and groove forms dark chamber, and body relative engagement face also is provided with the through hole face in upper and lower chamber, and two through hole face relative engagement forms the through hole that is communicated with dark chamber by the external world.

3, LED quick detector for photosynthetic capacity of irradiation according to claim 2 is characterized in that: the upper and lower chamber body in described darkroom is provided with the sealing gasket that is in the light in the position near the through hole face.

4, LED quick detector for photosynthetic capacity of irradiation according to claim 2 is characterized in that: the upper and lower chamber body in described darkroom is for splitting separated structures fully.

5, according to claim 2 or 4 described LED quick detector for photosynthetic capacity of irradiation, it is characterized in that: be provided with fixed sturcture between the upper and lower chamber body in described darkroom.

6, according to claim 2 or 4 described LED quick detector for photosynthetic capacity of irradiation, it is characterized in that: be provided with spring between the upper and lower chamber body in described darkroom.

7, LED quick detector for photosynthetic capacity of irradiation according to claim 1 is characterized in that: described darkroom is an integral structure, is provided with live body in the side, darkroom and stretches into the hole.

8, LED quick detector for photosynthetic capacity of irradiation according to claim 7 is characterized in that: the position of stretching into the hole near live body in the described darkroom is provided with the sealing gasket that is in the light.

Images