CN110794154A - Chemiluminescence immunity analyzer and detection method thereof - Google Patents
Chemiluminescence immunity analyzer and detection method thereof Download PDFInfo
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- CN110794154A CN110794154A CN201911103346.3A CN201911103346A CN110794154A CN 110794154 A CN110794154 A CN 110794154A CN 201911103346 A CN201911103346 A CN 201911103346A CN 110794154 A CN110794154 A CN 110794154A
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- 230000036039 immunity Effects 0.000 title description 2
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- 230000007246 mechanism Effects 0.000 claims abstract description 152
- 238000011534 incubation Methods 0.000 claims abstract description 73
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 64
- 238000003018 immunoassay Methods 0.000 claims abstract description 47
- 238000004140 cleaning Methods 0.000 claims abstract description 39
- 238000007885 magnetic separation Methods 0.000 claims abstract description 34
- 238000002156 mixing Methods 0.000 claims abstract description 30
- 239000000758 substrate Substances 0.000 claims abstract description 14
- 239000000523 sample Substances 0.000 claims description 188
- 239000007788 liquid Substances 0.000 claims description 61
- 238000002347 injection Methods 0.000 claims description 40
- 239000007924 injection Substances 0.000 claims description 40
- 238000005259 measurement Methods 0.000 claims description 31
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/76—Chemiluminescence; Bioluminescence
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5302—Apparatus specially adapted for immunological test procedures
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00584—Control arrangements for automatic analysers
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
- G01N35/025—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations having a carousel or turntable for reaction cells or cuvettes
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
- G01N35/04—Details of the conveyor system
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N2035/00346—Heating or cooling arrangements
- G01N2035/00356—Holding samples at elevated temperature (incubation)
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- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
- G01N35/04—Details of the conveyor system
- G01N2035/0439—Rotary sample carriers, i.e. carousels
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Abstract
The invention relates to the field of medical instruments, in particular to a chemiluminescence immunoassay analyzer, a reaction cup loading mechanism places a plurality of reaction cups in a cup receiving tray, a reaction cup conveying mechanism conveys the reaction cups in the cup receiving tray to an incubation tray, a sample adding mechanism sucks a sample and adds the sample to the reaction cups in the incubation tray, a sample adding mechanism sucks a reagent and adds the reagent to the reaction cups in the incubation tray, a reaction cup conveying mechanism conveys the reaction cups added with the sample and the reagent to a blending device for blending, the reaction cups are conveyed to a magnetic separation cleaning module by the reaction cup conveying mechanism for magnetic separation cleaning, a substrate is added into the reaction cups by the magnetic separation cleaning device, so that the reaction cups added with the substrate are conveyed to a photon measuring device by the reaction cup conveying mechanism for photometric detection, the analyzer can automatically process and detect the sample, and avoids the individual difference of manual operation, The uncertainty problems such as artificial error and irregular operation, and the detection accuracy of the chemiluminescence immunoassay is improved.
Description
Technical Field
The invention relates to the field of medical instruments, in particular to a chemiluminescence immunoassay analyzer and a detection method thereof.
Background
In the field of in vitro diagnostics, chemiluminescence immunoassay is an analytical technique for quantitatively detecting various antigens, haptens, antibodies, hormones, enzymes, fatty acids, vitamins, drugs, and the like by combining a chemiluminescence assay method with high sensitivity and a high-specificity immunoreaction method. The analytical testing process broadly comprises: firstly, respectively placing a sample and a reagent in a sample tray and a reagent tray, then adding the sample and the reagent in a reaction cup, respectively carrying out incubation, cleaning and the like on the reaction cup, and finally, putting the reaction cup in a detection tray to finish the determination. At present, chemiluminescence immunoassay analyzers in the industry generally have the problems that the operation of replacing reagents and placing sample tubes by users is complicated, the reaction cup loading is inconvenient, and the like. Moreover, most of detection instruments in the industry adopt a mode of manual test operation and semi-automatic detection, the detection instruments only realize a detection reading function, and the manual operation has uncertainty such as individual difference, manual error and nonstandard operation, so that the defects are exposed under the condition of a large number of samples and a large number of test items. Therefore, how to provide a full-automatic chemiluminescence immunoassay analyzer with high automation degree and rapid and accurate detection is a technical problem which needs to be solved urgently in the industry.
Disclosure of Invention
In order to solve the above problems, the present invention provides a chemiluminescence immunoassay analyzer, which solves the problems of low analysis efficiency and large detection error caused by manual intervention of the existing immunoassay analyzer.
Based on the above, the invention provides a chemiluminescence immunoassay analyzer, which comprises a rack, and a reaction cup loading mechanism, a sample introduction mechanism, an incubation disc, a reagent disc, a magnetic separation cleaning device, a uniform mixing device, a photon measuring device, a sample adding mechanism and a reaction cup conveying mechanism which are arranged on the rack;
the reaction cup loading mechanism comprises a funnel, a cup fishing structure, a cup separating structure and a cup receiving structure, the funnel is arranged on the frame, the cup separating structure comprises a cup guide piece, a supporting plate and a cup pushing block, the cup pushing block is slidably arranged on the supporting plate, the guide cup piece is provided with a guide cup groove, the support plate is provided with a sliding groove and a dropping hole, the sliding groove is communicated with the guide cup groove and the dropping hole, the cup receiving structure comprises a cup receiving tray which is rotationally connected with the frame, the cup receiving tray is provided with a plurality of reaction cup falling holes, the bailing cup block of the bailing cup structure moves relative to the funnel to scoop the reaction cup in the funnel to the cup guide groove, the reaction cups fall into the sliding chute through the cup guide groove, and the cup pushing block slides relative to the supporting plate to push the reaction cups in the sliding chute into the falling holes and fall into the cup receiving tray;
a plurality of sample containers are arranged on the sample injection mechanism;
the incubation disc and the reagent disc are both rotationally connected to the rack, the incubation disc is used for loading reaction cups, and a plurality of reagent containers are arranged in the reagent disc;
the magnetic separation cleaning device comprises a supporting seat, a magnet mounting disc, a reaction cup tray, a needle frame, a first driving mechanism and a second driving mechanism, wherein the magnet mounting disc is arranged at the top of the supporting seat; the magnet mounting disc is provided with a plurality of first magnets and a plurality of second magnets, and the first magnets and the second magnets are circumferentially distributed around the central axis of the magnet mounting disc at intervals; the second magnet is positioned on the outer layer of the first magnet; the reaction cup tray is rotatably connected to the top end of the supporting seat and is positioned above the magnet mounting disc; the first driving mechanism is used for driving the reaction cup tray to rotate; the needle frame is arranged on the supporting seat and is positioned above the reaction cup tray; the needle frame can move along the axial direction of the magnet mounting disc under the driving of the second driving mechanism; the needle frame is provided with a liquid suction needle assembly and a liquid injection needle assembly;
the mixing device is used for mixing the sample and the reagent in the reaction cup;
the photon measuring device comprises a measuring darkroom, a photon counter and a waste liquid absorbing mechanism; the measurement darkroom is provided with an inlet and an outlet, a waste liquid suction port, a reaction liquid inlet and a photometric channel; the measurement darkroom is internally provided with a measurement conveying mechanism, a cup carrying block and a shading block, and the cup carrying block is used for moving along the conveying direction of the measurement conveying mechanism under the conveying action of the measurement conveying mechanism; the photometric channel is arranged below the reaction liquid inlet and is positioned in the conveying direction of the measuring and conveying mechanism; the photon counter is arranged outside the measurement darkroom, and a probe of the photon counter corresponds to one end of the photometric channel; the other end of the photometric channel is communicated to the inside of the measurement darkroom; the light shielding block can move close to or far away from the end part of the light measuring channel; the light shielding block is used for plugging the end part of the light measuring channel after moving towards the end part close to the light measuring channel; the shading block is positioned on the motion track of the cup carrying block and is used for moving to the end part far away from the photometric channel under the driving of the cup carrying block, and the waste liquid suction mechanism extends into the measurement darkroom from a waste liquid suction port;
the sample adding mechanism is used for conveying the sample in the sample container and the reagent in the reagent container to the reaction cup on the incubation tray;
the reaction cup conveying mechanism is used for conveying the reaction cups among the incubation tray, the reaction cup tray, the inlet and the outlet of the measurement darkroom and the cup receiving tray.
Preferably, the cup separating structure further comprises a cup pushing driving part, a cup pushing rotating shaft and a cup pushing driving part, the cup pushing driving part is fixedly installed on the supporting plate, the cup pushing rotating shaft is rotatably installed on the supporting plate and is fixedly connected with the cup pushing driving part, and the cup pushing block is fixedly installed on the cup pushing driving part.
Preferably, the reaction cup comprises a hanging lug, the width of the sliding groove is smaller than the diameter of the hanging lug, and the diameter of the falling hole is larger than the diameter of the hanging lug.
Preferably, the sample feeding mechanism comprises a sample conveying device and a plurality of sample carrying devices arranged in parallel, the plurality of sample containers are arranged on the sample carrying devices, the sample conveying device comprises a first sample feeding guide rail and a sample feeding piece movably connected to the first sample feeding guide rail in a matched manner, and the sample feeding piece is detachably connected to the sample carrying devices.
Preferably, the sample conveying device further comprises a second sample feeding guide rail fixedly connected to the rack, the sample carrying devices are arranged in parallel along the length direction of the second sample feeding guide rail, the first sample feeding guide rail and the second sample feeding guide rail are perpendicular to each other, and the first sample feeding guide rail is movably connected to the second sample feeding guide rail in a matching manner.
Preferably, the injection needle assembly comprises a first injection needle and a second injection needle; the first liquid injection needle and the second liquid injection needle are fixedly connected to the needle frame at intervals; the bottom ends of the needle tubes of the first liquid injection needle and the second liquid injection needle are respectively provided with a plurality of liquid injection holes; the plurality of liquid injection holes are arranged at intervals along the height direction of the needle frame.
Preferably, the liquid suction needle assembly comprises a first liquid suction needle and a second liquid suction needle; the first liquid suction needle and the second liquid suction needle are fixedly connected to the needle frame at intervals and are positioned between the first liquid injection needle and the second liquid injection needle.
Preferably, the sample adding mechanism comprises a sample adding needle and a sample adding arm rotatably connected to the frame, and the sample adding needle is arranged at the tail end of the sample adding arm.
Preferably, the reaction cup conveying mechanism comprises a fixed structure, a moving structure and a clamping structure, the moving structure and the clamping structure are slidably mounted on the fixed structure, the fixed structure is fixedly connected to the frame, the clamping structure is connected to the moving structure, the moving structure comprises a driving member support, the clamping structure comprises a fixed clamping portion, an elastic member and a movable clamping portion, the fixed clamping portion is fixedly connected to the driving member support, the movable clamping portion comprises a gripper finger driving member, an eccentric wheel, a movable mounting block and a movable gripper finger, the gripper finger driving member is fixedly connected to the driving member support and connected with the eccentric wheel, the movable mounting block is rotatably mounted on the driving member support and fixedly connected with the movable gripper finger, two ends of the elastic member are respectively connected with the fixed clamping portion and the movable mounting block to keep the fixed clamping portion and the movable gripper finger in a clamping state, the gripper finger driving piece drives the eccentric wheel to rotate and abut against the movable mounting block, so that the movable gripper fingers move to enable the fixed clamping part and the movable gripper fingers to be in an open state.
In order to solve the same technical problem, the invention also provides a detection method of the chemiluminescence immunoassay analyzer, which comprises the following steps:
step S1, the reaction cup conveying mechanism conveys the reaction cups in the cup receiving tray to an incubation tray, and the incubation tray rotates to enable the reaction cups to be close to the sample adding mechanism;
step S2, the sample adding mechanism transports the sample in the sample container to the reaction cup in the incubation tray;
step S3, the sample adding mechanism conveys the reagent in the reagent container to the reaction cup in the incubation disc;
step S4, the reaction cup conveying mechanism conveys the reaction cups in the incubation disc to a blending device for blending, and the reaction cup conveying mechanism conveys the reaction cups on the blending device to the incubation disc;
step S5, the reaction cup conveying mechanism conveys the reaction cups in the incubation disc to a reaction cup tray of a magnetic separation cleaning device for magnetic separation cleaning;
step S6, the cuvette conveying mechanism conveys the cuvette on the cuvette tray to the incubation tray, and the incubation tray rotates to make the cuvette close to the sample adding mechanism;
step S7, the sample adding mechanism transports the reagent in the reagent container to the reaction cup in the incubation tray;
step S8, the reaction cup conveying mechanism conveys the reaction cups in the incubation tray to the reaction cup tray for magnetic separation and cleaning, and the magnetic separation cleaning device adds a first substrate to the reaction cups;
step S9, the reaction cup conveying mechanism conveys the reaction cups on the reaction cup tray to the incubation tray for incubation;
and S10, conveying the reaction cups in the incubation disc to an inlet and an outlet of a photon measuring device by the reaction cup conveying mechanism, and adding a second substrate to the reaction cups by the photon measuring device to perform photometric detection.
The invention relates to a chemiluminescence immunoassay analyzer, a reaction cup loading mechanism of the chemiluminescence immunoassay analyzer regularly places a plurality of reaction cups in a cup receiving tray, a reaction cup conveying mechanism conveys the reaction cups in the cup receiving tray to an incubation tray, a sample adding mechanism sucks samples in sample containers of a sample feeding mechanism and adds the samples to the reaction cups in the incubation tray, a sample adding mechanism sucks reagents in the reagent containers of the reagent tray and adds the reagents to the reaction cups in the incubation tray, a reaction cup conveying mechanism conveys the reaction cups added with the samples and the reagents to a mixing device for mixing, the mixed reaction cups can be conveyed to a magnetic separation cleaning module by the reaction cup conveying mechanism according to experimental conditions for magnetic separation cleaning, a substrate is added into the reaction cups by the magnetic separation cleaning device, so that the reaction cups added with the substrate are conveyed to a photon measuring device for photometric detection by the reaction cup conveying mechanism, the analyzer can automatically process and detect the samples, the method does not need manual intervention, avoids the uncertainty problems of individual difference, manual error, irregular operation and the like of manual operation, and improves the detection accuracy of chemiluminescence immunoassay.
Drawings
FIG. 1 is a schematic perspective view of a chemiluminescent immunoassay analyzer of an embodiment of the present invention;
FIG. 2 is a schematic top view of a chemiluminescent immunoassay analyzer of an embodiment of the present invention;
FIG. 3 is a schematic view of a cup structure of a chemiluminescent immunoassay analyzer of an embodiment of the present invention;
FIG. 4 is one of schematic top views of a cup structure of a chemiluminescent immunoassay analyzer of an embodiment of the present invention;
FIG. 5 is a second schematic top view of the cup structure of the chemiluminescence immunoassay analyzer according to the embodiment of the invention;
FIG. 6 is a schematic view of a cup receiving structure of a chemiluminescent immunoassay analyzer of an embodiment of the present invention;
FIG. 7 is a schematic view showing the overall structure of a reaction cup loading mechanism of the chemiluminescent immunoassay analyzer of the embodiment of the present invention;
FIG. 8 is a schematic view of a sample injection mechanism of a chemiluminescent immunoassay analyzer of an embodiment of the present invention;
FIG. 9 is a schematic structural diagram of a first sample guide rail and a second sample guide rail of a chemiluminescent immunoassay analyzer according to an embodiment of the present invention;
FIG. 10 is a schematic view of a sample carrier of a chemiluminescent immunoassay analyzer of an embodiment of the present invention;
FIG. 11 is a schematic view of a magnetic separation cleaning apparatus of a chemiluminescent immunoassay analyzer of an embodiment of the present invention;
FIG. 12 is a schematic structural view of a photon measuring apparatus of a chemiluminescent immunoassay analyzer of an embodiment of the present invention;
FIG. 13 is a schematic rear view of a photon measuring device of the chemiluminescent immunoassay analyzer of an embodiment of the present invention;
FIG. 14 is an exploded schematic view of a photon measuring device of a chemiluminescent immunoassay analyzer of an embodiment of the present invention;
FIG. 15 is a schematic perspective view of a reaction cup transport mechanism of a chemiluminescent immunoassay analyzer of an embodiment of the present invention;
FIG. 16 is an exploded view of the cuvette transport mechanism of the chemiluminescent immunoassay analyzer of an embodiment of the present invention;
FIG. 17 is a schematic view showing the internal structure of a cuvette transport mechanism of a chemiluminescent immunoassay analyzer of an embodiment of the present invention;
FIG. 18 is a schematic flow chart of a detection method of the chemiluminescence immunoassay analyzer according to the embodiment of the invention.
Wherein, 1, a reaction cup loading mechanism; 11. a funnel; 12. a fishing cup structure; 121. fishing a cup block; 13. a cup separating structure; 131. a guide cup member; 1311. a cup guide groove; 132. a support plate; 1321. a chute; 1322. dropping the hole; 133. pushing the cup block; 134. a cup pushing driving member; 135. a cup pushing rotating shaft; 136. a cup pushing transmission part; 14. a cup receiving structure; 141. a cup receiving tray; 141. the reaction cup falls into the hole; 2. a sample introduction mechanism; 21. a sample container; 22. a sample delivery device; 221. a first sample introduction guide rail; 222. a second sample introduction guide rail; 223. a sample inlet piece; 2231. connecting a clamping block; 23. a sample carrier; 231. a connecting clamping groove; 3. an incubation tray; 4. a reagent tray; 41. a reagent container; 5. a magnetic separation cleaning device; 51. a supporting seat; 52. a magnet mounting plate; 53. a reaction cup tray; 54. a needle frame; 55. a first drive mechanism; 56. a second drive mechanism; 6. a blending device; 7. a photon measuring device; 71. a measurement darkroom; 711. an inlet and an outlet; 712. a waste liquid suction port; 713. a reaction liquid inlet; 714. a light measuring channel; 72. a photon counter; 73. a waste liquid absorbing mechanism; 74. a measuring and conveying mechanism; 75. a cup carrying block; 76. a light shielding block; 8. a sample adding mechanism; 81. a sample adding needle; 82. a sample addition arm; 9. a reaction cup conveying mechanism; 91. a fixed structure; 92. a moving structure; 921. a driving member support; 922. a position sensor; 93. a clamping structure; 931. a fixed clamping part; 932. an elastic member; 933. a movable clamping part; 9331. a finger grip drive; 9332. an eccentric wheel; 9333. a movable mounting block; 9334. a movable gripper finger; 9335. a baffle plate; 10. and a frame.
Detailed Description
The following detailed description of embodiments of the present invention is provided in connection with the accompanying drawings and examples. The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention.
Referring to fig. 1 to 17, a chemiluminescence immunoassay analyzer of the present invention is schematically shown, which includes a rack 10, and a cuvette loading mechanism 1, a sample introduction mechanism 2, an incubation disk 3, a reagent disk 4, a magnetic separation and cleaning device 5, a mixing device 6, a photon measurement device 7, a sample addition mechanism 8, and a cuvette conveying mechanism 9, which are disposed on the rack 10.
Reaction cup loading mechanism 1 includes funnel 11, drag for cup structure 12, divide cup structure 13 and cup structure 14, frame 10 is located to funnel 11, divide cup structure 13 to include and lead cup spare 131, backup pad 132 and push away cup piece 133, push away cup piece 133 slidable mounting in backup pad 132, it leads cup piece 131 and is equipped with and leads cup groove 1311, backup pad 132 is equipped with spout 1321 and drop hole 1322, spout 1321 and lead cup groove 1311 and drop hole 1322 communicate, cup structure 14 includes and connects cup plate 141, connect cup plate 141 to rotate and connect in the installation shell, connect cup plate 141 to be equipped with a plurality of reaction cup and fall into hole 141, drag for cup piece 121 of drag for cup structure 12 and move with respect to funnel 11 and drag for the reaction cup in funnel 11 to lead cup groove 1311, and fall into spout 1321 through leading cup groove 1311, push away cup piece 133 and slide with respect to backup pad 132 and push the reaction cup in spout 1321 into and drop the hole and fall into cup plate 141.
The sample introduction mechanism 2 is provided with a plurality of sample containers 21.
The incubation disc 3 and the reagent disc 4 are both rotatably connected to the frame 10, the incubation disc 3 is used for loading reaction cups, and a plurality of reagent containers 41 are arranged in the reagent disc 4.
The magnetic separation cleaning device 5 comprises a supporting seat 51, a magnet mounting disc 52, a reaction cup tray 53, a needle frame 54, a first driving mechanism 55 and a second driving mechanism 56, wherein the magnet mounting disc 52 is arranged at the top of the supporting seat 51; the magnet mounting disc 52 is provided with a plurality of first magnets and a plurality of second magnets, and the plurality of first magnets and the plurality of second magnets are circumferentially distributed around the central axis of the magnet mounting disc 52 at intervals; the second magnet is positioned on the outer layer of the first magnet; the reaction cup tray 53 is rotatably connected to the top end of the support base 51 and positioned above the magnet mounting plate 52; the first driving mechanism 55 is used for driving the reaction cup tray 53 to rotate; the needle frame 54 is arranged on the supporting seat 51 and is positioned above the reaction cup tray 53; the needle frame 54 can move along the axial direction of the magnet mounting disc 52 under the driving of a second driving mechanism 56; the needle holder 54 is provided with a liquid suction needle assembly and a liquid injection needle assembly.
The blending device 6 is used for blending the sample and the reagent in the reaction cup, and the blending device 6 can adopt the existing reagent shaking device and the like.
The photon measuring device 7 comprises a measuring darkroom 71, a photon counter 72 and a waste liquid absorbing mechanism 73; the measurement darkroom 71 is provided with an inlet and an outlet 711, a waste liquid suction port 712, a reaction liquid inlet 713 and a photometric channel 714; a measurement conveying mechanism 74, a cup carrying block 75 and a shading block 76 are arranged in the measurement darkroom 71, and the cup carrying block 75 is used for moving along the conveying direction of the measurement conveying mechanism 74 under the conveying action; the photometric channel 714 is provided below the reaction liquid inlet 713 and in the conveying direction of the measuring and conveying mechanism 74; the photon counter 72 is arranged outside the measurement darkroom 71, and a probe of the photon counter 72 corresponds to one end of the photometric channel 714; the other end of the photometric channel 714 is communicated to the inside of the measurement darkroom 71; the light shielding block 76 is movable toward or away from the end of the photometric channel 714; the light shielding block 76 is used for blocking the end of the photometric channel 714 after moving toward the end close to the photometric channel 714; the light shielding block 76 is located on the motion track of the cup carrying block 75 and is used for moving towards the end far away from the light measuring channel 714 under the driving of the cup carrying block 75, and the waste liquid suction mechanism 73 extends into the measurement darkroom 71 from the waste liquid suction port 712. After being filled into a reaction cup, a measuring reagent can be placed on the cup carrying block 75 through the inlet and outlet 711, and sequentially reaches the waste liquid suction port 712 to suck waste liquid under the driving of the measuring and conveying mechanism 74, after the measuring and conveying mechanism 74 is further conveyed to the reaction liquid inlet 713 to add reaction liquid, the photometric channel 714 is opened to carry out photon measurement, the operation is continuous, and the measuring efficiency is high; and the whole process is carried out in the measurement darkroom 71, the light-shielding effect is higher, and the measurement accuracy is higher.
The sample adding mechanism 8 is used for transferring the sample in the sample container 21 and the reagent in the reagent container 41 to the reaction cup on the incubation tray 3.
The cuvette transfer mechanism 9 is used to transfer cuvettes between the incubation tray 3, the cuvette tray 53, the entrance 711 and the exit 711 of the measurement dark room 71, and the cuvette receiving tray 141.
The operation flow of the analyzer is roughly as follows: the reaction cup loading mechanism 1 regularly places a plurality of reaction cups in the cup receiving tray 141, the reaction cup conveying mechanism 9 conveys the reaction cups in the cup receiving tray 141 to the incubation tray 3, the sample adding mechanism 8 absorbs the samples in the sample containers 21 of the sample introduction mechanism 2 and adds the samples to the reaction cups in the incubation tray 3, the sample adding mechanism 8 absorbs the reagents in the reagent containers 41 of the reagent tray 4 and adds the reagents to the reaction cups in the incubation tray 3, the reaction cup conveying mechanism 9 conveys the reaction cups added with the samples and the reagents to the mixing device 6 for mixing, the mixed reaction cups can be conveyed to the magnetic separation cleaning module by the reaction cup conveying mechanism 9 for magnetic separation cleaning according to experimental conditions, the magnetic separation cleaning device 5 adds substrates into the reaction cups, so that the reaction cup conveying mechanism 9 conveys the reaction cup added with the substrate to the photon measuring device 7 for photometric detection.
The chemiluminescence immunoassay analyzer can automatically process and detect samples without manual intervention, avoids the uncertainty problems of individual difference, manual error, irregular operation and the like of manual operation, and improves the detection accuracy of chemiluminescence immunoassay.
Referring to fig. 3 to 7, the cup separating structure 13 of the reaction cup loading mechanism 1 further includes a cup pushing driving member 134, a cup pushing rotating shaft 135 and a cup pushing driving member 136, the cup pushing driving member 134 is fixedly mounted on the supporting plate 132, the cup pushing rotating shaft 135 is rotatably mounted on the supporting plate 132 and is fixedly connected to the cup pushing driving member 134, and the cup pushing block 133 is fixedly mounted on the cup pushing driving member 136. The reaction cup comprises a hanging lug, the width of the sliding groove 1321 is smaller than the diameter of the hanging lug, and the diameter of the falling hole 1322 is larger than the diameter of the hanging lug.
Reaction cup loading mechanism 1 still includes the installation shell, and the installation shell includes first panel, second panel, base and fixed block. The first panel and the second panel are mounted on the base and are parallel to each other. The fixed block is fixed with the first panel and the second panel and is positioned at the other end opposite to the base.
The drag cup structure 12 includes a roller, a plurality of drag cup blocks 121, a roller driving member, and a roller driving member. The roller is rotatably arranged between the first panel and the second panel, and each roller is provided with a plurality of grooves. Each scoop block 121 includes a body, an extension post, and a hinge projection. The extension post extends out from the main part, and the hinge joint arch is located the extension post. The main body is also provided with a hinge hole. In this embodiment, the hinge protrusion extends into the hinge hole to form a hinge, so that the two baicup blocks 121 form a hinge. The plurality of bailing cup blocks 121 are hinged together to form an annular belt and are sleeved on the roller. The rotation of the roller can drive each fishing cup block 121 to move.
The roller driving member of the cup catching structure 12 drives the roller to rotate, so that the cup catching block 121 moves, the cup catching block 121 can catch the reaction cup, and the reaction cup falls from the cup guiding groove 1311 to the chute 1321 when the cup catching block 121 moves to the cup guiding groove 1311. Since the width of the slide 1321 is smaller than the diameter of the ear of the cuvette. The reaction cup is suspended in the chute 1321 by a suspension loop. The cup pushing driving member 134 drives the cup pushing block 133 to slide relative to the supporting plate 132. The pushing portion of the cup pushing block 133 pushes the reaction cup in the chute 1321 to the drop hole 1322. Since the drop hole 1322 has a diameter larger than that of the suspension loop. The reaction cups are dropped into the cup receiving tray 141 through the drop holes 1322.
With reference to fig. 8 to 10, the sample injection mechanism 2 includes a sample conveying device 22 and a plurality of sample carrying devices 23 arranged in parallel, the sample carrying devices 23 are detachably connected to the rack 10, and a plurality of sample containers 21 are arranged on the sample carrying devices 23 in a straight line; the sample conveying device 22 includes a first sample introduction guide rail 221 and a sample introduction member 223 movably connected to the first sample introduction guide rail 221 in a matching manner, so that the sample introduction member 223 can slide on the first sample introduction guide rail 221, the sample introduction member 223 is detachably connected to the sample carrying device 23, specifically, one end of the sample carrying device 23 is provided with a connection clamping groove 231, the sample introduction member 223 is provided with a screw rod and a nut movably connected to the screw rod in a matching manner, the nut is provided with a connection clamping block 2231, when the screw rod rotates, the nut can drive the connection clamping block 2231 to move axially along the screw rod and enable the connection clamping block 2231 to be embedded into or withdrawn from the connection clamping groove 231, so that the sample introduction member 223 is connected to or separated from the. After the sample feeding member 223 is connected to the sample loading device 23, the sample feeding member 223 slides along the first sample feeding guide rail 221 to drive the sample loading device 23 to move, so that the sample loading device 23 moves toward the sample feeding mechanism 8 for sample feeding.
Further, the sample conveying device 22 further includes a second sample guide rail 222 fixedly connected to the rack 10, the plurality of sample carrying devices 23 are arranged in parallel along a length direction of the second sample guide rail 222, the first sample guide rail 221 is perpendicular to the second sample guide rail 222, and the first sample guide rail 221 is movably connected to the second sample guide rail 222 in a matching manner, wherein the first sample guide rail 221 and the second sample guide rail 222 are uniformly arranged on a horizontal plane, so that the sample piece 223 can move along two mutually perpendicular directions in a horizontal direction, and further the sample piece 223 can be connected to different sample carrying devices 23, and the sample carrying device 23 is conveyed to one side of the sample adding mechanism 8.
Be equipped with a plurality of first mounting holes on incubating dish 3, the reaction cup can inlay and locate in first mounting hole, and first mounting hole has heating function, satisfies the environment of incubating of constant temperature 37 ℃, and wherein, the heating function accessible of first mounting hole is current heating device realization such as current heater strip.
The reagent disk 4 is provided with a plurality of second mounting holes, and the reagent containers 41 can be inserted into the second mounting holes.
As shown in fig. 11, the injection needle assembly of the magnetic separation cleaning device 5 includes a first injection needle and a second injection needle; first notes liquid needle and second annotate liquid needle interval fixed connection on needle frame 54, the needle tubing bottom of first notes liquid needle and second notes liquid needle all is equipped with a plurality of notes liquid holes, and a plurality of notes liquid holes are arranged along the direction of height interval of needle frame 54. The liquid suction needle assembly comprises a first liquid suction needle and a second liquid suction needle, and the first liquid suction needle and the second liquid suction needle are fixedly connected to the needle frame 54 at intervals and are positioned between the first liquid injection needle and the second liquid injection needle. Two layers of magnets are distributed on the magnet mounting disc 52, cleaning liquid can be injected into the reaction cup on the reaction cup tray 53 through the liquid injection needle assembly, the reaction cup tray 53 rotates, and the inner layer of magnets and the outer layer of magnets on the magnet mounting disc 52 can alternately perform inner and outer adsorption, dispersion and cleaning on magnetic combination materials in the reaction cup, so that the magnetic materials move in the cleaning liquid for multiple times and are adsorbed in multiple stages, the cleaning effect is improved, and the loss rate of magnetic beads is reduced.
The sample adding mechanism 8 includes a sample adding needle 81 and a sample adding arm 82 rotatably connected to the frame 10, the sample adding needle 81 is provided at the end of the sample adding arm 82, and the rotatable sample adding arm 82 can move the sample adding needle 81 to the upper side of the sample container 21, the reagent container 41 or the reaction cup on the incubation plate 3. Further, still be equipped with the application of sample installation sleeve pipe of vertical setting in the frame 10, the pivot of application of sample arm 82 is located to application of sample installation sleeve pipe cover for application of sample arm 82's pivot can be followed the relative application of sample installation sleeve pipe of application of sample installation sheathed tube axial and slided, in order to realize application of sample needle 81's raising and lowering functions, makes application of sample needle 81 can stretch into sample container 21, reagent container 41 or incubate the reaction cup on the dish 3.
As shown in fig. 15 to 17, the reaction cup conveying mechanism 9 includes a fixed structure 91, a movable structure 92 slidably mounted on the fixed structure 91, and a clamping structure 93, the fixed structure 91 is fixedly connected to the frame 10, the clamping structure 93 is connected to the movable structure 92, the movable structure 92 includes a driving member seat 921, the clamping structure 93 includes a fixed clamping portion 931, an elastic member 932 and a movable clamping portion 933, the fixed clamping portion 931 is fixedly connected to the driving member seat 921, the movable clamping portion 933 includes a finger gripping driving member 9331, an eccentric 9332, a movable mounting block 9333, a movable gripping finger 9334, the finger gripping driving member 9331 is fixedly connected to the driving member seat 921 and connected to the eccentric 9332, the movable mounting block 9333 is rotatably mounted to the driving member seat 921 and fixedly connected to the movable gripping finger 9334, two ends of the elastic member 932 are respectively connected to the fixed clamping portion 931 and the movable mounting block 9333 to maintain the fixed clamping portion 931 and the movable gripping portion 9334 in a clamping state, the finger driving member 9331 drives the eccentric wheel 9332 to rotate and abut against the movable mounting block 9333, so that the movable finger 9334 moves to enable the fixed clamping part 931 and the movable finger 9334 to be in an open state.
The movable clamping part 933 further comprises a blocking piece 9335, the blocking piece 9335 is fixed on the movable mounting block 9333, the moving structure 92 further comprises a position sensor 922, the position sensor 922 is fixedly mounted on the driving piece support 921, the blocking piece 9335 moves along with the movable mounting block 9333 to be close to or far away from the blocking piece 9335, and the position sensor 922 receives or cannot receive a detection signal so as to judge whether the electric control mechanical gripper is in a clamping state or an opening state.
In order to solve the same technical problem, as shown in fig. 18, the present invention further provides a detection method of a chemiluminescence immunoassay analyzer, comprising the following steps:
step S1, the cuvette conveying mechanism 9 conveys the cuvettes in the cuvette receiving tray 141 to the incubation tray 3, and the incubation tray 3 rotates to make the cuvettes close to the sample adding mechanism 8;
step S2, the sample adding mechanism 8 transfers the sample in the sample container 21 to the reaction cup in the incubation tray 3;
step S3, the sample adding mechanism 8 transfers the reagent in the reagent container 41 to the cuvette in the incubation tray 3;
step S4, the reaction cup conveying mechanism 1 conveys the reaction cups in the incubation disc 3 to the blending device 6 for blending, and the reaction cup conveying mechanism 1 conveys the reaction cups on the blending device 6 to the incubation disc 3;
step S5, the cuvette conveying mechanism 1 conveys the cuvettes in the incubation tray 3 to the cuvette tray 53 of the magnetic separation cleaning device 5 for magnetic separation cleaning;
step S6, the cuvette conveying mechanism 1 conveys the cuvettes on the cuvette tray 53 to the incubation tray 3, and the incubation tray 3 rotates to make the cuvettes close to the sample adding mechanism 8;
step S7, the sample adding mechanism 8 transfers the reagent in the reagent container 41 to the cuvette in the incubation tray 3;
step S8, the cuvette conveying mechanism 1 conveys the cuvettes in the incubation tray 3 to the cuvette tray 53 for magnetic separation cleaning, and the magnetic separation cleaning device 5 adds the first substrate to the cuvettes;
step S9, the reaction cup conveying mechanism 1 conveys the reaction cups on the reaction cup tray 53 to the incubation plate 3 for incubation;
step S10, the cuvette conveying mechanism 1 conveys the cuvettes in the incubation tray 3 to the inlet and outlet of the photon measuring device 7, and the photon measuring device 7 adds the second substrate to the cuvettes for photometric detection.
It should be noted that, in step S4, the reaction cup after being uniformly mixed by the uniformly mixing device 6 can execute a two-step method according to the actual detection condition for detection and analysis, specifically: when the two-step method is required to be performed on the uniformly mixed reaction cup, the steps after the step S5 and the step S5 are continuously performed; when the two-step method is not required to be performed on the well-mixed reaction cup, the steps S5 to S7 are skipped and the steps S8 and the steps after S8 are performed directly.
In summary, in the chemiluminescence immunoassay analyzer of the present invention, the cuvette loading mechanism 1 regularly places a plurality of cuvettes in the cuvette receiving tray 141, the cuvette conveying mechanism 9 conveys the cuvettes in the cuvette receiving tray 141 to the incubation tray 3, the sample adding mechanism 8 sucks the sample in the sample container 21 of the sample introducing mechanism 2 and adds the sample to the cuvettes in the incubation tray 3, the sample adding mechanism 8 sucks the reagent in the reagent container 41 of the reagent tray 4 and adds the reagent to the cuvettes in the incubation tray 3, the cuvette conveying mechanism 9 conveys the cuvettes with the sample and the reagent added thereto to the mixing device 6 for mixing, the mixed cuvettes can be conveyed to the magnetic separation cleaning module by the cuvette conveying mechanism 9 according to the experimental conditions for magnetic separation cleaning, the magnetic separation cleaning device 5 adds the substrate to the cuvettes, so that the cuvette conveying mechanism 9 conveys the cuvettes with the substrate added thereto to the photon measuring device 7 for photometric detection, the analyzer can automatically process and detect samples without manual intervention, avoids the uncertainty problems of individual difference, manual error, nonstandard operation and the like of manual operation, and improves the detection accuracy of chemiluminescence immunoassay.
The above description is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and substitutions can be made without departing from the technical principle of the present invention, and these modifications and substitutions should also be regarded as the protection scope of the present invention.
Claims (10)
1. A chemiluminescence immunoassay analyzer is characterized by comprising a rack, a reaction cup loading mechanism, a sample introduction mechanism, an incubation disc, a reagent disc, a magnetic separation cleaning device, a uniform mixing device, a photon measuring device, a sample adding mechanism and a reaction cup conveying mechanism, wherein the reaction cup loading mechanism, the sample introduction mechanism, the incubation disc, the reagent disc, the magnetic separation cleaning device, the uniform mixing device, the photon measuring device, the sample adding mechanism and the reaction cup conveying mechanism are;
the reaction cup loading mechanism comprises a funnel, a cup fishing structure, a cup separating structure and a cup receiving structure, the funnel is arranged on the frame, the cup separating structure comprises a cup guide piece, a supporting plate and a cup pushing block, the cup pushing block is slidably arranged on the supporting plate, the guide cup piece is provided with a guide cup groove, the support plate is provided with a sliding groove and a dropping hole, the sliding groove is communicated with the guide cup groove and the dropping hole, the cup receiving structure comprises a cup receiving tray which is rotationally connected with the frame, the cup receiving tray is provided with a plurality of reaction cup falling holes, the bailing cup block of the bailing cup structure moves relative to the funnel to scoop the reaction cup in the funnel to the cup guide groove, the reaction cups fall into the sliding chute through the cup guide groove, and the cup pushing block slides relative to the supporting plate to push the reaction cups in the sliding chute into the falling holes and fall into the cup receiving tray;
a plurality of sample containers are arranged on the sample injection mechanism;
the incubation disc and the reagent disc are both rotationally connected to the rack, the incubation disc is used for loading reaction cups, and a plurality of reagent containers are arranged in the reagent disc;
the magnetic separation cleaning device comprises a supporting seat, a magnet mounting disc, a reaction cup tray, a needle frame, a first driving mechanism and a second driving mechanism, wherein the magnet mounting disc is arranged at the top of the supporting seat; the magnet mounting disc is provided with a plurality of first magnets and a plurality of second magnets, and the first magnets and the second magnets are circumferentially distributed around the central axis of the magnet mounting disc at intervals; the second magnet is positioned on the outer layer of the first magnet; the reaction cup tray is rotatably connected to the top end of the supporting seat and is positioned above the magnet mounting disc; the first driving mechanism is used for driving the reaction cup tray to rotate; the needle frame is arranged on the supporting seat and is positioned above the reaction cup tray; the needle frame can move along the axial direction of the magnet mounting disc under the driving of the second driving mechanism; the needle frame is provided with a liquid suction needle assembly and a liquid injection needle assembly;
the mixing device is used for mixing the sample and the reagent in the reaction cup;
the photon measuring device comprises a measuring darkroom, a photon counter and a waste liquid absorbing mechanism; the measurement darkroom is provided with an inlet and an outlet, a waste liquid suction port, a reaction liquid inlet and a photometric channel; the measurement darkroom is internally provided with a measurement conveying mechanism, a cup carrying block and a shading block, and the cup carrying block is used for moving along the conveying direction of the measurement conveying mechanism under the conveying action of the measurement conveying mechanism; the photometric channel is arranged below the reaction liquid inlet and is positioned in the conveying direction of the measuring and conveying mechanism; the photon counter is arranged outside the measurement darkroom, and a probe of the photon counter corresponds to one end of the photometric channel; the other end of the photometric channel is communicated to the inside of the measurement darkroom; the light shielding block can move close to or far away from the end part of the light measuring channel; the light shielding block is used for plugging the end part of the light measuring channel after moving towards the end part close to the light measuring channel; the shading block is positioned on the motion track of the cup carrying block and is used for moving to the end part far away from the photometric channel under the driving of the cup carrying block, and the waste liquid suction mechanism extends into the measurement darkroom from a waste liquid suction port;
the sample adding mechanism is used for conveying the sample in the sample container and the reagent in the reagent container to the reaction cup on the incubation tray;
the reaction cup conveying mechanism is used for conveying the reaction cups among the incubation tray, the reaction cup tray, the inlet and the outlet of the measurement darkroom and the cup receiving tray.
2. The chemiluminescent immunoassay analyzer of claim 1, wherein the cup separating structure further comprises a cup pushing driving member, a cup pushing rotating shaft and a cup pushing transmission member, wherein the cup pushing driving member is fixedly mounted on the supporting plate, the cup pushing rotating shaft is rotatably mounted on the supporting plate and is fixedly connected with the cup pushing driving member, and the cup pushing block is fixedly mounted on the cup pushing transmission member.
3. The chemiluminescent immunoassay analyzer of claim 1, wherein the reaction cup comprises a tab, the chute has a width less than a diameter of the tab, and the drop hole has a diameter greater than the diameter of the tab.
4. The chemiluminescent immunoassay analyzer of claim 1, wherein the sample injection mechanism comprises a sample delivery device and a plurality of sample bearing devices arranged in parallel, the plurality of sample containers are disposed on the sample bearing devices, the sample delivery device comprises a first sample injection guide rail and a sample injection member movably connected to the first sample injection guide rail in a matching manner, and the sample injection member is detachably connected to the sample bearing devices.
5. The chemiluminescent immunoassay analyzer of claim 4, wherein the sample conveying device further comprises a second sample guide rail fixedly connected to the rack, the plurality of sample carrying devices are arranged in parallel along the length direction of the second sample guide rail, the first sample guide rail and the second sample guide rail are perpendicular to each other, and the first sample guide rail is movably connected to the second sample guide rail in a matching manner.
6. The chemiluminescent immunoassay analyzer of claim 1, wherein the injection needle assembly comprises a first injection needle and a second injection needle; the first liquid injection needle and the second liquid injection needle are fixedly connected to the needle frame at intervals; the bottom ends of the needle tubes of the first liquid injection needle and the second liquid injection needle are respectively provided with a plurality of liquid injection holes; the plurality of liquid injection holes are arranged at intervals along the height direction of the needle frame.
7. The chemiluminescent immunoassay analyzer of claim 1, wherein the pipette needle assembly comprises a first pipette needle and a second pipette needle; the first liquid suction needle and the second liquid suction needle are fixedly connected to the needle frame at intervals and are positioned between the first liquid injection needle and the second liquid injection needle.
8. The chemiluminescent immunoassay analyzer of claim 1, wherein the sample application mechanism comprises a sample application needle and a sample application arm rotatably connected to the frame, wherein the sample application needle is arranged at the end of the sample application arm.
9. The chemiluminescent immunoassay analyzer of claim 1, wherein the reaction cup conveying mechanism comprises a fixed structure, a moving structure and a clamping structure, the moving structure and the clamping structure are slidably mounted on the fixed structure, the fixed structure is fixedly connected to the frame, the clamping structure is connected to the moving structure, the moving structure comprises a driving member support, the clamping structure comprises a fixed clamping portion, an elastic member and a movable clamping portion, the fixed clamping portion is fixedly connected to the driving member support, the movable clamping portion comprises a gripper finger driving member, an eccentric wheel, a movable mounting block and a movable gripper finger, the gripper finger driving member is fixedly connected to the driving member support and connected to the eccentric wheel, the movable mounting block is rotatably mounted to the driving member support and fixedly connected to the movable gripper finger, and two ends of the elastic member are respectively connected to the fixed clamping portion and the movable mounting block to maintain the fixed clamping portion and the movable mounting block The fixed clamping part and the movable gripper fingers are in a clamping state, the gripper finger driving piece drives the eccentric wheel to rotate and abut against the movable mounting block, and the movable gripper fingers move to enable the fixed clamping part and the movable gripper fingers to be in an opening state.
10. A detection method of a chemiluminescence immunoassay analyzer is characterized by comprising the following steps:
step S1, the reaction cup conveying mechanism conveys the reaction cups in the cup receiving tray to an incubation tray, and the incubation tray rotates to enable the reaction cups to be close to the sample adding mechanism;
step S2, the sample adding mechanism transports the sample in the sample container to the reaction cup in the incubation tray;
step S3, the sample adding mechanism conveys the reagent in the reagent container to the reaction cup in the incubation disc;
step S4, the reaction cup conveying mechanism conveys the reaction cups in the incubation disc to a blending device for blending, and the reaction cup conveying mechanism conveys the reaction cups on the blending device to the incubation disc;
step S5, the reaction cup conveying mechanism conveys the reaction cups in the incubation disc to a reaction cup tray of a magnetic separation cleaning device for magnetic separation cleaning;
step S6, the cuvette conveying mechanism conveys the cuvette on the cuvette tray to the incubation tray, and the incubation tray rotates to make the cuvette close to the sample adding mechanism;
step S7, the sample adding mechanism transports the reagent in the reagent container to the reaction cup in the incubation tray;
step S8, the reaction cup conveying mechanism conveys the reaction cups in the incubation tray to the reaction cup tray for magnetic separation and cleaning, and the magnetic separation cleaning device adds a first substrate to the reaction cups;
step S9, the reaction cup conveying mechanism conveys the reaction cups on the reaction cup tray to the incubation tray for incubation;
and S10, conveying the reaction cups in the incubation disc to an inlet and an outlet of a photon measuring device by the reaction cup conveying mechanism, and adding a second substrate to the reaction cups by the photon measuring device to perform photometric detection.
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