CN110658350B - Electric pipettor - Google Patents
Electric pipettor Download PDFInfo
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- CN110658350B CN110658350B CN201910547417.2A CN201910547417A CN110658350B CN 110658350 B CN110658350 B CN 110658350B CN 201910547417 A CN201910547417 A CN 201910547417A CN 110658350 B CN110658350 B CN 110658350B
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- 239000007788 liquid Substances 0.000 claims abstract description 25
- 230000001360 synchronised effect Effects 0.000 claims abstract description 11
- 238000002347 injection Methods 0.000 claims description 38
- 239000007924 injection Substances 0.000 claims description 38
- 230000003750 conditioning effect Effects 0.000 claims description 22
- 238000005070 sampling Methods 0.000 claims description 16
- 230000003287 optical effect Effects 0.000 claims description 15
- 238000001514 detection method Methods 0.000 claims description 14
- 238000007789 sealing Methods 0.000 description 7
- 238000006073 displacement reaction Methods 0.000 description 6
- 239000000523 sample Substances 0.000 description 5
- 238000012423 maintenance Methods 0.000 description 4
- 239000012472 biological sample Substances 0.000 description 3
- 230000000903 blocking effect Effects 0.000 description 3
- 238000003780 insertion Methods 0.000 description 3
- 230000037431 insertion Effects 0.000 description 3
- 230000035945 sensitivity Effects 0.000 description 3
- 230000007547 defect Effects 0.000 description 2
- 238000005213 imbibition Methods 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 239000012620 biological material Substances 0.000 description 1
- 239000003638 chemical reducing agent Substances 0.000 description 1
- 238000012864 cross contamination Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1009—Characterised by arrangements for controlling the aspiration or dispense of liquids
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- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
本发明提供了一种电动移液器，包括：加载机构，包括具有固定支点的支撑架、第一螺杆、从动带轮、通过同步带与从动带轮连接的主动带轮及第一电机；执行机构，位于固定支点前侧，包括机架、注射管、竖直插设于注射管内的柱塞、与柱塞连接的错位滑块、与错位滑块螺纹连接的第二螺杆及第二电机，第一螺杆通过转动驱动机架带动注射管轴向移动，注射管通过轴向向下移动与移液头插接，第二螺杆通过转动驱动错位滑块带动柱塞轴向移动，柱塞通过轴向移动将液体吸入或排出移液头。本发明采用常规电机，能实现移液执行机构往复运动轴线与机构支点垂线距离最短化，减轻由于移液执行机构沿轴线向下与移液头频繁进行压入式连接时产生的有害倾覆力矩和疲劳变形。
The present invention provides an electric pipette, comprising: a loading mechanism, comprising a support frame with a fixed fulcrum, a first screw, a driven pulley, a driving pulley connected to the driven pulley through a synchronous belt, and a first motor; an actuator, located in front of the fixed fulcrum, comprising a frame, a syringe, a plunger vertically inserted in the syringe, a dislocation slider connected to the plunger, a second screw threadedly connected to the dislocation slider, and a second motor, the first screw drives the frame to drive the syringe axially, the syringe moves downward axially and plugs into the pipetting head, the second screw drives the dislocation slider to drive the plunger axially, and the plunger sucks or discharges the liquid into or out of the pipetting head through axial movement. The present invention adopts a conventional motor, which can minimize the distance between the reciprocating motion axis of the pipetting actuator and the vertical line of the mechanism fulcrum, and reduce the harmful overturning moment and fatigue deformation caused by the frequent press-in connection between the pipetting actuator and the pipetting head along the axis downward.
Description
Technical Field
The invention relates to sample adding equipment, in particular to an electric pipettor.
Background
In detection work in the fields of medicine, food, biology and the like, a plurality of pretreatment works are needed to be carried out on biological materials and samples (usually in liquid state), and the operations of pipetting, sample adding, mixing and the like can be finished in batches in a short time by adopting high-throughput automatic biological sample pretreatment equipment, so that an electric pipetting device is a core technical unit in an automatic biological sample treatment system. The electric pipettors are classified into two types, gas-liquid mixed displacement type and gas displacement type, according to the suction driving method of the electric pipettors on the liquid.
The gas-liquid mixing displacement type liquid-transfering device adopts a liquid plunger, and separates the liquid plunger and sample liquid by an air gap reserved in the liquid-transfering head, and has the advantages that an electric reciprocating motion injection mechanism, a pressure sensor and the like are separated, only one thin guide pipe enters a liquid-transfering actuating mechanism, so that the design pressure and load of a liquid-transfering actuating mechanism assembly are greatly reduced, the minimum center distance requirement of a vessel is met, meanwhile, the distance between the reciprocating motion axis of the liquid-transfering actuating mechanism and the vertical line of a mechanism fulcrum is minimized, the harmful overturning moment and the fatigue deformation of a supporting part generated when the liquid-transfering actuating mechanism is frequently connected with the liquid-transfering head in a pressing mode along the axis of the liquid-transfering actuating mechanism are reduced, and the liquid-transfering precision is high because the separation air gap between the liquid plunger and the sample liquid is small. The system has the defects of gas-liquid pipeline pressure system, more components, complex debugging and high maintenance cost, and is easy to generate liquid leakage when the room temperature is low.
The gas displacement type pipettor adopts a solid plunger, the reciprocating motion of the solid plunger is realized by a motor through a shaft coupler or other connecting parts driving screw rods or synchronous toothed belts, that is to say, the components such as an electric reciprocating motion injection mechanism, a pressure sensor and the like are assembled with a pipetting executing mechanism together, a complex pressure pipeline and a pump system are not needed, the structure level is clear, and the workload of installing and debugging the unit structure is greatly reduced. The first existing gas displacement type liquid-transfering device adopts a small-diameter high-rotation-speed motor with a speed reducer to form a blade type liquid-transfering actuating mechanism unit, although the distance between the reciprocating motion axis of the liquid-transfering actuating mechanism and the vertical line of a mechanism fulcrum is minimized, the cost is extremely high, the maintenance cost is high, the debugging difficulty is high, the second existing gas displacement type liquid-transfering device adopts a conventional motor, the cost is low, the maintenance cost is low, the debugging is convenient, but the defect is that the distance between the reciprocating motion axis of the liquid-transfering actuating mechanism and the vertical line of the mechanism fulcrum is increased, the generated harmful overturning moment is increased when the liquid-transfering actuating mechanism is frequently connected with a liquid-transfering head in a pressing mode along the axis of the liquid-transfering actuating mechanism, and the supporting part is easy to generate fatigue deformation.
Disclosure of Invention
The invention aims to provide an electric liquid-transfering device which can solve the problem of high price existing in the prior art that a small-diameter high-rotation-speed motor is adopted, and can also solve the problems of large harmful overturning moment and fatigue deformation generated when a liquid-transfering actuating mechanism is frequently connected with a liquid-transfering head in a pressing-in mode that the distance between the reciprocating motion axis of the liquid-transfering actuating mechanism and the vertical line of a mechanism fulcrum is large and the liquid-transfering actuating mechanism is downwards along the axis.
The invention provides an electric pipettor which comprises a loading mechanism, an executing mechanism and a driving mechanism, wherein the loading mechanism comprises a support frame with a fixed fulcrum, a first screw rod which is rotatably and vertically arranged on the support frame and positioned at the front side of the fixed fulcrum, a driven belt wheel which is connected above the first screw rod, a driving belt wheel which is connected with the driven belt wheel through a horizontally arranged synchronous belt, and a first motor which is coaxially connected with the driving belt wheel, the first motor is positioned at the rear side of the fixed fulcrum, the first motor can drive the first screw rod to rotate through the driving belt wheel, the synchronous belt and the driven belt wheel in sequence, the executing mechanism is positioned at the front side of the fixed fulcrum, and comprises a frame which can be vertically and slidably arranged on the support frame, an injection tube which is vertically fixed on the frame and can be spliced with a pipetting head, a plunger which is vertically inserted in the injection tube, a dislocation slide block which is arranged above the injection tube and connected with the plunger, a second motor which is rotatably and vertically arranged on the frame and is connected with the dislocation slide block, the first motor can drive the first screw rod to axially move through the second screw rod, the second screw rod is axially arranged above the driving screw rod and the second screw rod to axially move the injection head to the injection tube through the second screw rod, and the second screw rod is axially displaced by the second screw rod to the second screw rod.
The electric pipettor comprises a syringe, a plunger, a pressure detection device, a pressure sensor, a sampling tube and a connecting rod, wherein the lower end of the syringe is provided with an inserting wall which can be inserted into the pipette, a perforation is arranged on the side wall of the syringe, which is adjacent to the inserting wall, the perforation is positioned above the inserting wall and below the plunger, the pressure detection device comprises the sampling tube and the pressure sensor arranged on the frame, one end of the sampling tube is inserted into the perforation and is communicated with the syringe, and the other end of the sampling tube is connected with the pressure sensor.
The electric pipette as described above, wherein the actuator further comprises a tubular bumper inserted into the end of the syringe, the bumper being located below the plunger.
The electric pipette as described above, wherein the actuating mechanism further includes a pipette head withdrawing device, the pipette head withdrawing device includes a pressing block capable of axially sliding and sleeved outside the injection tube, a push rod fixed above the pressing block and capable of axially moving and vertically installed on the frame, an elastic member sleeved outside the push rod, and an adjusting ring fixedly sleeved outside the push rod, the adjusting ring is located above the elastic member, the adjusting ring and the elastic member are separated by the frame, the upper end of the elastic member abuts against the frame, the lower end of the elastic member abuts against the pressing block, the push rod is located below the dislocating slide block, the dislocating slide block pushes the push rod and the pressing block to apply pressure to the top end of the pipette head sleeved outside the injection tube through downward movement, so that the pipette head falls from the injection tube, and the adjusting ring abuts against the frame when the push rod moves downward by a preset distance.
The electric pipettor comprises a pressing block, wherein the pressing block is arranged on the upper portion of the pressing block, a first baffle is arranged at the top of the pressing block, a second baffle is arranged at the bottom of the dislocation sliding block, a signal conditioning plate is arranged at the lower portion of the machine frame and positioned below the dislocation sliding block and above the pressing block, a plunger position sensor for detecting the position of the plunger and a pipetting head position sensor for detecting the position of the pipetting head are arranged on the signal conditioning plate, the pipetting head position sensor detects the position of the pipetting head by detecting the position of the first baffle, and the plunger position sensor detects the position of the plunger by detecting the position of the second baffle.
The electric pipettor, wherein the actuating mechanism further comprises an optical axis which is fixed on the frame and is parallel to the second screw rod, a linear bearing which is arranged in the dislocation slide block, and an anti-backlash nut which is arranged in the dislocation slide block, the optical axis passes through the linear bearing, and the second screw rod passes through the anti-backlash nut and is in threaded connection with the dislocation slide block through the anti-backlash nut.
The electric pipettor comprises a staggered sliding block, wherein the staggered sliding block is internally provided with a connecting hole which is coaxially arranged with a plunger, the lower end of the connecting hole is provided with a conical hole with the diameter gradually expanding from bottom to top, the top end of the plunger is connected with a connecting rod, the top end of the connecting rod is connected with a ball head, the ball head is positioned in the connecting hole and sits on the conical hole, the diameter of the connecting rod is smaller than the minimum diameter of the conical hole, and a rubber plug is fixed in the connecting hole and positioned above the ball head and props against the ball head.
The electric pipettor comprises a rack, a bottom frame arranged below the rack, and a side plate connected between the rack and the top frame, wherein the top frame, the side plate and the bottom frame enclose a sliding space, a second motor is fixed at the top of the top frame, a second screw rod penetrates through the sliding space, the upper end of the second screw rod is connected with the second motor, the lower end of the second screw rod is connected with the bottom frame through a bearing, a dislocation slide block is arranged in the sliding space, and an injection tube penetrates through the bottom frame and is fixedly connected with the bottom frame.
The electric pipettor comprises a support frame, a first screw rod, a connecting plate, a fixing fulcrum and a nut slider, wherein the support frame is a strip-shaped hollow groove block, the first screw rod is arranged in the groove block, the front side of the groove block is open, the rear side plate of the groove block is a connecting plate which is vertically arranged, the fixing fulcrum is positioned on the connecting plate, a vertical guide rail is arranged in the groove block, one side of the frame, which faces the groove block, is fixedly provided with the guide rail slider and the nut slider, the guide rail slider stretches into the groove block and is in sliding fit with the guide rail, the nut slider stretches into the groove block and is in threaded connection with the first screw rod, and the first screw rod drives the frame and the guide rail slider to slide up and down along the guide rail through rotation.
The electric pipettor comprises a support frame, wherein the support frame comprises a connecting plate, a driving belt wheel, a driven belt wheel, a bearing, a first motor, a first screw rod, a bearing, a bottom plate and a driving belt wheel, wherein the support frame further comprises the top plate and the bottom plate, the top plate is fixed at the top of the connecting plate, the bottom plate is fixed at the bottom of the connecting plate, the driving belt wheel is installed at the top of the top plate, the top plate extends towards the rear side of the connecting plate to form an extension plate, the driven belt wheel is installed at the top of the extension plate, the first motor is inversely fixed at the bottom of the extension plate, the upper end of the first screw rod is connected with the top plate through the bearing, and the lower end of the first screw rod is connected with the bottom plate through the bearing.
The electric pipettor has the characteristics and advantages that:
1. The electric pipettor adopts the conventional motor, compared with the motor with small diameter and high rotating speed in the prior art, the electric pipettor has lower price, because the size of the conventional motor is larger, when the electric pipettor is arranged, the first motor is arranged at the rear side of the fixed pivot, the driving belt wheel, the synchronous belt and the driven belt wheel which are positioned above the first screw rod are adopted to transmit the torque of the first motor to the first screw rod, the actuating mechanism is arranged at the front side of the fixed pivot, and the second motor is arranged above the second screw rod to directly drive the second screw rod to rotate, so that the first motor and the second motor cannot occupy the space between the reciprocating axis of the actuating mechanism and the fixed pivot excessively, the shortest vertical line distance between the reciprocating axis of the actuating mechanism and the fixed pivot can be realized, and the harmful overturning moment and fatigue deformation generated when the actuating mechanism is frequently connected with the pipetting head in a pressing mode along the axis downwards are lightened;
2. According to the electric pipette, the sampling point of the pressure sensor is arranged at the narrow air gap at the joint of the injection cavity in the injection tube and the pipette head, and the sampling air gap is very small, so that under the cooperation of the high-sensitivity pressure sensor, when impurities or clots in liquid block the pipette head, the detection is easy, and the sensitivity of pressure detection is effectively improved;
3. according to the electric pipette, the pressing block of the pipette head withdrawing device is directly sleeved outside the injection tube, the ejector rod and the pressing block are driven by the dislocation sliding block to move downwards, so that the pipette head can be withdrawn, the driving device of the plunger is used as the driving device of the pressing block, the structure is simple and compact, a pipette head withdrawing mechanism with a complex structure and a driving electromagnetic component are not required, and the cost is reduced;
4. according to the electric liquid-transfering device, the injection tube can be directly spliced with the liquid-transfering head, so that the injection pump and the injection cavity are integrated, the structure is simple and compact, the injection tube and the plunger are lengthened, and the range is enlarged;
5. the electric pipettor has the advantages of simple and compact structure, low price, low operation, maintenance cost and convenient debugging.
Drawings
The following drawings are only for purposes of illustration and explanation of the present invention and are not intended to limit the scope of the invention. Wherein:
FIG. 1 is a front view of one embodiment of an motorized pipette of the present invention;
FIG. 2 is a cross-sectional view of an actuator of the present invention;
fig. 3 is a schematic perspective view of one embodiment of the motorized pipettor of the present invention.
Reference numerals for main elements:
100. a loading mechanism;
101. Support frame, 1011, connecting plate, 1012, top plate, 1013, bottom plate, 1014, extension plate;
102. 103, a driven belt pulley 104 and a synchronous belt;
105. Driving pulley, 106, first motor, 107, guide rail, 108, reference position sensor;
109. a fixed fulcrum;
200. An actuator;
201. 2011, a top frame, 2012, a bottom frame, 2013, side plates, 2014 and a mounting groove;
2015. 2016, wire slot, 2017, sliding space;
202. 2021, splicing walls, 2022, perforations, 2023 and annular protrusions;
2024. An O-shaped sealing ring;
203. plunger 204, dislocation slide block 205, second screw rod;
206. the device comprises a second motor, 207, a buffer, 208, a sampling tube, 209, a pressing block, 210 and a push rod;
211. the elastic piece 212, the adjusting ring 213, the first jackscrew 214, the second jackscrew 215 and the first baffle;
216. The signal conditioning board 217, the second baffle plate 218, the optical axis 219, the connecting rod 220 and the ball head;
221. rubber plug, 222, adjusting nut, 223, O-shaped sealing ring, 224, hollow flange;
225. guide rail slide block, 226, nut slide block, 227, gap eliminating nut;
300. A pipetting head.
Detailed Description
For a clearer understanding of technical features, objects, and effects of the present invention, a specific embodiment of the present invention will be described with reference to the accompanying drawings. In the description of the present invention, it should be understood that the terms "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are merely for convenience in describing the present invention and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying a relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first", "a second", etc. may explicitly or implicitly include one or more such feature. In the description of the present invention, unless otherwise indicated, the meaning of "a plurality" is two or more.
Unless defined otherwise, references herein to "front" are to the left as shown in fig. 1 of the present invention and to "rear" are to the right as shown in fig. 1 of the present invention, and references herein to "up", "down", "vertical", "horizontal", etc. are to the top, bottom, vertical, horizontal, etc. as shown in fig. 1 of the present invention, and are hereby incorporated by reference.
As shown in fig. 1,2 and 3, the present invention proposes an electric pipette which can be mounted on a work rack, the electric pipette comprising a loading mechanism 100 for loading a pipette head 300 and an actuator 200 for sucking the pipette, wherein the pipette head 300 is a standardized pipette head with a volume of 50 microliter to 1000 microliter, the pipette head 300 is a disposable, is generally made of plastic, belongs to consumables, is discarded after one liquid treatment is completed, wherein,
The loading mechanism 100 includes a support frame 101 having a fixed fulcrum 109 (or referred to as a mechanism fulcrum), or the connection of the support frame 101 and the work frame forms a fixed fulcrum for supporting the electric pipettor;
The loading mechanism 100 further comprises a first screw 102 which is rotatably and vertically arranged on the support frame 101 and positioned at the front side of the fixed pivot 109, a driven belt pulley 103 connected above the first screw 102, a driving belt pulley 105 connected with the driven belt pulley 103 through a horizontally arranged synchronous belt 104, and a first motor 106 coaxially connected with the driving belt pulley 105, wherein the first motor 106, the driving belt pulley 105 and the driven belt pulley 103 are all arranged on the support frame 101, the first motor 106 is positioned at the rear side of the fixed pivot 109, and the first motor 106 can drive the first screw 102 to rotate through the driving belt pulley 105, the synchronous belt 104 and the driven belt pulley 103 in sequence, and the first motor 106 is provided with an encoder;
the actuator 200 is located in front of the fixed fulcrum 109, the actuator 200 comprises a rack 201 which is installed on the supporting frame 101 and can slide up and down, a syringe 202 which is vertically fixed on the rack 201 and can be inserted into the pipette head 300, a plunger 203 which is vertically inserted into the syringe 202, a dislocation slide block 204 which is arranged above the syringe 202 and is connected with the plunger 203, a second screw 205 which is rotatably and vertically installed on the rack 201 and is in threaded connection with the dislocation slide block 204, and a second motor 206 which is arranged above the second screw 205 and is connected with the top end of the second screw 205, an injection cavity is formed in the syringe 202, the second motor 206 can drive the second screw 205 to rotate, the second motor 206 is provided with an encoder, the first screw 102 drives the syringe 202 to move axially upwards or downwards through rotation of the rack 201, the syringe 202 is inserted into the pipette head 300 through axial downwards movement, so that the pipette head 300 is loaded, the second screw 205 drives the plunger 203 to move axially upwards or axially downwards through rotation of the dislocation slide block 204, the plunger 203 sucks liquid into the pipette head 300 through axial upwards movement, and the liquid is discharged through axial downwards through axial movement of the plunger 203, so that liquid is discharged from the pipette head 300 through axial movement.
When the automatic pipetting device is used, the electric pipetting device is arranged on the working frame, so that the electric pipetting device is suspended, various containers or detection equipment can be placed below the electric pipetting device, and pipetting, sample adding and other operations are completed through the pipetting head 300.
The electric pipettor of the invention adopts the first motor 106 and the second motor 206 which are common motors (conventional motors), and the price is low, because the common motors have larger size, when the electric pipettor is arranged, the first motor 106 is arranged at the rear side of the fixed pivot 109 (namely, the mechanism pivot), the driving pulley 105, the synchronous belt 104 and the driven pulley 103 which are positioned above the first screw 102 are adopted to transmit the torque of the first motor 106 to the first screw 102, the actuating mechanism 200 is arranged at the front side of the fixed pivot 109, and the second motor 206 is arranged above the second screw 205 to directly drive the second screw 205 to rotate, therefore, the first motor 106 and the second motor 206 do not occupy the space between the reciprocating motion axis of the actuating mechanism 200 (namely, the central axes of the second motor 206 and the second screw 205) and the fixed pivot 109 excessively, the shortest perpendicular distance between the reciprocating motion axis of the actuating mechanism 200 and the fixed pivot 109 can be realized, and the harmful covering moment and fatigue deformation which are generated when the actuating mechanism 200 is frequently connected with the pipette head 300 in a pressing mode along the axis are lightened;
In addition, by placing the first motor 106 having a large volume behind the fixed fulcrum 109, the left-right width of the portion of the electric pipette located on the front side of the fixed fulcrum 109 is reduced;
In addition, since the syringe 202 can be directly inserted into the pipette head 300, the combination of the syringe pump and the injection cavity is realized, so that the structure is simple and compact, and the syringe 202 and the plunger 203 are lengthened, thereby enlarging the range.
As shown in fig. 2, in one embodiment, the actuator 200 further includes a tubular buffer 207, the buffer 207 is inserted into the end of the syringe 202, that is, into the end of the injection cavity, the buffer 207 is located below the plunger 203, the gas in the buffer 207 acts as a gas plunger, and the inner diameter of the buffer 207 is smaller than the inner diameter of the injection cavity, so as to meet the requirement of high precision in a small range.
As shown in fig. 2, in one embodiment, the lower end of the syringe 202 has a plugging wall 2021 capable of being plugged into the pipette head 300, the plugging wall 2021 is connected with the pipette head 300 in a sealing manner, a through hole 2022 is formed on the side wall of the syringe 202 adjacent to the plugging wall 2021, the through hole 2022 is located above the plugging wall 2021 and below the plunger 203, the buffer 207 is plugged into the plugging wall 2021, the buffer 207 is located below the through hole 2022, the electric pipette further comprises a pressure detection device, the pressure detection device comprises a sampling tube 208 and a pressure sensor arranged on the rack 201, one end of the sampling tube 208 is plugged into the through hole 2022 and is communicated with the syringe 202, the other end of the sampling tube 208 is connected with the pressure sensor, and the sampling tube 208 is an elongated tube made of metal, for example, the pressure sensor is a micro-pressure sensor, and the sensitivity is high.
In this embodiment, the sampling point of the pressure sensor is disposed at a narrow air gap at the connection position of the injection cavity and the pipetting head 300 in the injection tube 202, and because the sampling air gap is very small, when the pipetting head 300 is blocked by impurities or clots in the liquid under the cooperation of the high-sensitivity pressure sensor, the pressure sensor is easy to detect, so that the sensitivity of pressure detection is effectively improved, and the pressure detection with high signal-to-noise ratio can also realize bubble artifact identification and liquid level detection, so that the main pressure data detection in the liquid treatment is fully satisfied.
As shown in fig. 2, specifically, the outer wall surface of the insertion wall 2021 has two annular protrusions 2023 arranged up and down, the cross section of the annular protrusions 2023 is arc-shaped, when the insertion wall 2021 is inserted into the pipette head 300, the annular protrusions 2023 are attached to the inner wall surface of the pipette head 300, and an O-shaped seal ring 2024 is disposed between the two annular protrusions 2023, so that a static seal is formed between the syringe 202 and the pipette head 300. When loading the pipette head 300, the first motor 106 drives the first screw 102 to rotate, and the first screw 102 drives the actuator 200 to move downwards through rotation until the two annular protrusions 2023 of the insertion wall 2021 of the syringe 202 are pressed into the pipette head 300, so that the operation of loading the pipette head 300 is completed.
As shown in fig. 1,2 and 3, in one embodiment, the actuator 200 further includes a pipette head withdrawing device, where the pipette head withdrawing device includes a pressing block 209 that is capable of sliding axially and sleeved outside the syringe 202, a push rod 210 that is fixed above the pressing block 209 and is capable of moving axially and vertically installed on a frame 201, an elastic member 211 that is sleeved outside the push rod 210, and an adjusting ring 212 that is fixedly sleeved outside the push rod 210, for example, the elastic member 211 is a spring, the adjusting ring 212 is located above the elastic member 211, and the adjusting ring 212 and the elastic member 211 are separated by the frame 201, an upper end of the elastic member 211 abuts against the frame 201, a lower end of the elastic member 211 abuts against a pressing block 209, the push rod 210 is located below the dislocating slider 204, and the dislocating slider 204 applies pressure to a top end of the pipette head 300 sleeved outside the syringe 202 by pushing the push rod 210 and the pressing block 209 downward, so that the pipette head 300 falls from the syringe 202, and the adjusting ring 212 can abut against the frame 201 when the push rod 210 moves downward by a preset distance.
In this embodiment, the pressing block 209 of the pipette head withdrawing device is directly sleeved outside the syringe 202, the ejector rod 210 and the pressing block 209 are driven by the dislocation slide block 204 to move downwards, so that the pipette head 300 can be withdrawn, the driving device of the plunger 203 (i.e., the dislocation slide block 204, the second screw 205 and the second motor 206) is directly used as the driving device of the pressing block 209, the structure is simple and compact, and the complicated structure of the pipette head withdrawing mechanism and the driving electromagnetic component are not required, so that the cost is reduced.
As shown in fig. 2, specifically, a first jackscrew 213 is provided on a side wall of the adjusting ring 212 along a radial direction, the first jackscrew 213 is in threaded connection with the adjusting ring 212 and abuts against a side wall of the push rod 210 to fix the adjusting ring 212 and the push rod 210 together, when the position of the adjusting ring 212 on the push rod 210 needs to be adjusted, the first jackscrew 213 is unscrewed, the adjusting ring 212 is moved up and down, the adjusting ring 212 is moved to a proper position, and then the first jackscrew 213 is screwed again to fix the adjusting ring 212 and the push rod 210 together, so that the adjustment is very convenient;
The second jackscrew 214 is arranged on the side wall of the pressing block 209 along the radial direction, the second jackscrew 214 is in threaded connection with the pressing block 209 and props against the side wall of the ejector rod 210 so as to fix the pressing block 209 and the ejector rod 210 together, when the relative position of the ejector rod 210 and the pressing block 209 needs to be adjusted, the second jackscrew 214 is unscrewed, the ejector rod 210 is moved up and down, the second jackscrew 214 is screwed again after the ejector rod 210 is moved to a proper position, and the ejector rod 210 and the pressing block 209 are fixed together, so that the adjustment is very convenient.
As shown in fig. 2, further, the top of the pressing block 209 is further provided with a first baffle 215, when the pressing block 209 pushes the pipetting head 300 downwards, the first baffle 215 can move downwards along with the pressing block 209, the lower part of the rack 201 is provided with a signal conditioning plate 216, the signal conditioning plate 216 is located above the pressing block 209, the signal conditioning plate 216 is provided with a pipetting head position sensor for detecting the position of the pipetting head 300, the pipetting head position sensor detects the position of the pipetting head 300 by detecting the position of the first baffle 215, and the signal conditioning plate 216 can transmit a position signal of the pipetting head 300 to a computer. For example, the pipetting head position sensor is a photoelectric sensor.
As shown in fig. 2, further, a second blocking piece 217 is further disposed at the bottom of the dislocation slide block 204, when the dislocation slide block 204 drives the plunger 203 to move up and down, the second blocking piece 217 can move up and down along with the dislocation slide block 204, the signal conditioning board 216 is located below the dislocation slide block 204, a plunger position sensor for detecting the position of the plunger 203 is disposed on the signal conditioning board 216, the plunger position sensor detects the position of the plunger 203 by detecting the position of the second blocking piece 217, and the signal conditioning board 216 can transmit a position signal of the plunger 203 to a computer. For example, the plunger position sensor is a photoelectric sensor.
As shown in fig. 1,2 and 3, in one embodiment, the actuator 200 further includes an optical axis 218 fixed on the frame 201 and parallel to the second screw 205, a linear bearing disposed in the dislocation slide 204, and an anti-backlash nut 227 disposed in the dislocation slide 204, where the optical axis 218 is used as a slide rail of the dislocation slide 204 and guides the dislocation slide 204 to slide, for example, the optical axis 218 is two, the two optical axes 218 are disposed at a side-by-side interval, the two linear bearings are two, each optical axis 218 passes through each linear bearing, and the second screw 205 passes through the anti-backlash nut 227 and is in threaded connection with the dislocation slide 204 through the anti-backlash nut 227. When the second motor 206 drives the second screw 205 to rotate, the anti-backlash nut 227 drives the dislocation slide block 204 to slide up and down along the optical axis 218.
As shown in fig. 2, in one embodiment, the plunger 203 is flexibly connected with the dislocation slide 204, specifically, a connecting hole coaxially arranged with the plunger 203 is provided in the dislocation slide 204, a tapered hole with a diameter gradually expanding from bottom to top is provided at the lower end of the connecting hole, a connecting rod 219 is connected to the top end of the plunger 203, a ball head 220 is connected to the top end of the connecting rod 219, the ball head 220 is located in the connecting hole and sits on the tapered hole, the diameter of the connecting rod 219 is smaller than the minimum diameter of the tapered hole, so that the connecting rod 219 can pass through the tapered hole, a rubber plug 221 is fixed in the connecting hole, and the rubber plug 221 is located above the ball head 220 and abuts against the ball head 220. By adopting such a connection structure, the plunger 203 and the dislocation slider 204 are relatively fixed in the axial direction, and the plunger 203 is allowed to shift moderately left and right while moving axially, and the plunger 203 is less likely to be damaged compared with a rigid connection.
As shown in fig. 2, further, the plunger 203 is slidably and sealingly connected with the top end of the syringe 202, specifically, a cap-shaped adjusting nut 222 is sleeved outside the top end of the syringe 202, the plunger 203 can axially slidably pass through the adjusting nut 222, an O-shaped sealing ring 223 is sandwiched between the top end of the syringe 202 and the top wall of the adjusting nut 222, the O-shaped sealing ring 223 is located between the side wall of the adjusting nut 222 and the plunger 203, and the O-shaped sealing ring 223 and the adjusting nut 222 can slidably and sealingly connect the syringe 202 and the plunger 203, so as to realize dynamic sealing.
As shown in fig. 1 and 3, in one embodiment, the rack 201 includes a top frame 2011, a bottom frame 2012 located below the top frame 2011, and a side plate 2013 connected between the top frame 2011 and the bottom frame 2012, wherein the top frame 2011 and the bottom frame 2012 are all block-shaped, the top frame 2011, the side plate 2013 and the bottom frame 2012 enclose a sliding space 2017, the second motor 206 is fixed on a hollow flange 224 at the top of the top frame 2011, the second screw 205 passes through the sliding space 2017, the upper end of the second screw 205 passes through the top frame 2011 and is connected with the second motor 206, the lower end of the second screw 205 is connected with the bottom frame 2012 through a bearing, for example, the second screw 205 is in an integral structure with a rotor of the second motor 206, an optical axis 218 passes through the sliding space 2017, the upper end of the optical axis 218 is fixedly connected with the bottom frame 2012, the misplaced slider 204 is located in the sliding space 2017, the injection tube 202 passes through the bottom frame 2012 and is fixedly connected with the bottom frame 2012, the upper end of the injection tube 202 stretches into the sliding space 2017, the lower end of the injection tube 210 passes through the sliding ring 2012, the lower end of the bottom frame 2012 is located above the bottom frame 2012, and the elastic member 2012 can move down on the bottom frame 211 when the bottom frame 2012 is located above the bottom frame 2012, and the elastic member 211 is located above the bottom frame 2012;
The bottom of the chassis 2012 is provided with a mounting groove 2014, the signal conditioning plate 216 is arranged in the mounting groove 2014 at the bottom of the chassis 2012, the chassis 2012 is also provided with a through hole 2015 which is penetrated up and down, the through hole 2015 is adjacent to and communicated with the mounting groove 2014, the first baffle 215 and the second baffle 217 are aligned up and down with the through hole 2015, the first baffle 215 can extend upwards into the through hole 2015 to be detected by a pipetting head position sensor on the signal conditioning plate 216, and the second baffle 217 can extend downwards into the through hole 2015 to be detected by a plunger 203 position sensor on the signal conditioning plate 216.
In a specific arrangement, for example, the injection tube 202, the ejector rod 210, the optical axis 218 and the second screw 205 are sequentially arranged in a direction approaching to the supporting frame 101, the two optical axes 218 are arranged side by side left and right, the first baffle 215 and the second baffle 217 are located at the rear side of the ejector rod 210 and the front side of the second screw 205, the signal conditioning plate 216 is located at the rear side of the first baffle 215 and the second baffle 217, the side plate 2013 of the rack 201 is located at the rear side of the chassis 2012, the signal conditioning plate 216 is located below the side plate 2013, a wire slot 2016 is arranged in the side plate 2013, the wire slot 2016 is communicated with a mounting slot 2014 where the signal conditioning plate 216 is located, the signal wire passes through the wire slot 2016, one end of the signal wire is connected with the signal conditioning plate 216, the other end of the signal wire is connected with the second motor 206, the signal conditioning plate 216 transmits a position signal of the plunger 203 to the second motor 206 through the signal wire, and the second motor 206 controls the rotation of the second screw 205 according to the position signal of the plunger 203, so as to control the initial position of the dislocation slide 204, and then control the position of the plunger 203.
As shown in fig. 1 and 3, in a specific embodiment, the support frame 101 is a hollow elongated slot block, the front side of the slot block is open, the rear side plate 2013 of the slot block is a vertically arranged connecting plate 1011, the fixing fulcrum 109 is located on the connecting plate 1011, specifically, the working frame is located at the rear side of the connecting plate 1011, a fixing fulcrum 109 is formed at the connection position between the rear side surface of the connecting plate 1011 and the working frame, a vertical guide rail 107 is disposed in the slot block, for example, the guide rail 107 is fixed on the front side surface of the connecting plate 1011, the first screw 102 is disposed in the slot block, a guide rail slide 225 and a nut slide 226 are fixed on one side of the frame 201 facing the slot block, the guide rail slide 225 extends into the slot block and is in sliding fit with the guide rail 107, the nut slide 226 extends into the slot block and is in threaded connection with the first screw 102, and the nut slide 226 drives the frame 201 and the guide rail slide 225 to slide up and down along the guide rail 107 when the first screw 102 rotates. The combination of the support frame 101 and the frame 201 in this embodiment is simple and compact.
For example, two guide rail sliders 225 are provided at an interval from top to bottom, the upper guide rail slider 225 is fixed to the rear side of the top frame 2011 of the frame 201, the lower guide rail slider 225 is fixed to the rear side of the connecting plate 1011 of the frame 201, the nut slider 226 is located between the two guide rail sliders 225, and the nut slider 226 is fixed to the lower guide rail slider 225. Preferably, the nut sliding block 226 is an anti-backlash nut, and the frame 201 and the guide rail sliding block 225 are of an integrated structure, so that the structure strength is high.
In one embodiment, as shown in fig. 2, the trough block further includes a top plate 1012 and a bottom plate 1013, the top plate 1012 is fixed on top of the connection plate 1011, the bottom plate 1013 is fixed on bottom of the connection plate 1011, the driving pulley 105 is mounted on top of the top plate 1012, the top plate 1012 extends toward the rear side of the connection plate 1011 to form an extension plate 1014, the driven pulley 103 is mounted on top of the extension plate 1014, the first motor 106 is inversely fixed on bottom of the extension plate 1014, the upper end of the first screw 102 is connected with the top plate 1012 through a bearing, and the lower end of the first screw 102 is connected with the bottom plate 1013 through a bearing. Specifically, the top plate 1012 and the bottom plate 1013 are flanges, which are convenient for connection.
As shown in fig. 2, further, the upper part of the channel block is further provided with a reference position sensor 108 for detecting the position of the frame 201 in the vertical direction, the reference position sensor 108 is located below the top plate 1012 of the channel block and adjacent to the top plate 1012 of the channel block, and the reference position sensor 108 is located above the two rail sliders 225, and the reference position sensor 108 can detect the position of the frame 201 when the frame 201 slides up and down along the rail 107.
When the electric pipette of the present invention is used for pipetting, in order to avoid cross contamination of liquid, the liquid cannot enter the injection cavity in the injection tube 202 and cannot enter the buffer 207, and can only be in the pipetting head 300, so that the range of liquid treatment depends on the volume of the pipetting head 300, and the range of the electric pipette depends on the volume of the plunger 203.
Before imbibition, the position of the plunger 203 needs to be initialized, specifically, the dislocation slide block 204 drives the plunger 203 to move downwards, once the signal conditioning plate 216 detects the position of the second baffle 217 on the dislocation slide block 204, a zero signal is output to the second motor 206, the second motor 206 controls the dislocation slide block 204 to stop moving through the second screw 205, the position is recorded as the initial position (reset) of the dislocation slide block 204 and the plunger 203, and the plunger 203 starts to move upwards during imbibition;
When the pipetting head 300 is required to be withdrawn after one pipetting operation is completed, the dislocating slide 204 is allowed to overrun downwards by a few millimeters, at this time, the dislocating slide 204 drives the ejector rod 210 downwards, the ejector rod 210 and the pressing block 209 fixedly connected together move downwards, the pipetting head 300 is withdrawn, and the initial position is re-detected (reset) before the next pipetting operation.
The foregoing is illustrative of the present invention and is not to be construed as limiting the scope of the invention. Any equivalent changes and modifications can be made by those skilled in the art without departing from the spirit and principles of this invention, and are intended to be within the scope of this invention. It should be noted that, the components of the present invention are not limited to the above-mentioned overall application, and each technical feature described in the specification of the present invention may be selected to be used singly or in combination according to actual needs, so that other combinations and specific applications related to the present invention are naturally covered by the present invention.
Claims (8)
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| Application Number | Priority Date | Filing Date | Title |
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| CN201910547417.2A CN110658350B (en) | 2019-06-24 | 2019-06-24 | Electric pipettor |
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| CN201910547417.2A CN110658350B (en) | 2019-06-24 | 2019-06-24 | Electric pipettor |
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| CN110658350A CN110658350A (en) | 2020-01-07 |
| CN110658350B true CN110658350B (en) | 2024-11-29 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111482209B (en) * | 2020-05-07 | 2024-05-14 | 普瑞麦迪（北京）实验室技术有限公司 | Multi-range homobody pipette and pipette |
| CN111443214B (en) * | 2020-05-07 | 2024-11-29 | 普瑞麦迪（北京）实验室技术有限公司 | Electric pipetting device |
| CN111974472A (en) * | 2020-07-31 | 2020-11-24 | 杭州九洋生物科技有限公司 | Pipette and pipetting method |
| WO2022021377A1 (en) * | 2020-07-31 | 2022-02-03 | 杭州九洋生物科技有限公司 | Pipette and pipetting method |
| CN113578407A (en) * | 2021-07-12 | 2021-11-02 | 广东省科学院健康医学研究所 | Automatic change suction device |
| CN116532178B (en) * | 2023-07-06 | 2023-09-26 | 北京雷奥顶峰生物科技有限责任公司 | Device is changed to pipetting head |
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| CN210720436U (en) * | 2019-06-24 | 2020-06-09 | 普瑞麦迪（北京）实验室技术有限公司 | Electric liquid transfer device |
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| DE102007042115B4 (en) * | 2007-09-05 | 2010-12-02 | Eppendorf Ag | pipette |
| CN103551217A (en) * | 2013-11-06 | 2014-02-05 | 北京自由度科学机器有限公司 | Liquid transfer mechanism for full-automatic liquid transfer instrument and application thereof |
| CN103691498B (en) * | 2014-01-06 | 2015-05-27 | 广州市刑事科学技术研究所 | Liquid-moving machine and method of moving liquid by same |
| CN103861671A (en) * | 2014-03-21 | 2014-06-18 | 北京自由度科学机器有限公司 | Full-automatic pipetting mechanism and instrument |
| CN203874790U (en) * | 2014-03-21 | 2014-10-15 | 北京自由度科学机器有限公司 | Full-automatic pipetting workstation |
| PL3260822T3 (en) * | 2016-06-21 | 2021-02-08 | Eppendorf Ag | Multi-channel spray for use with a metering device |
| CN208865658U (en) * | 2018-08-01 | 2019-05-17 | 德诺杰亿（北京）生物科技有限公司 | Pipettor and liquid-transfering device |
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| CN210720436U (en) * | 2019-06-24 | 2020-06-09 | 普瑞麦迪（北京）实验室技术有限公司 | Electric liquid transfer device |
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