CN115888864A - Liquid transfer equipment for gene detection - Google Patents

Abstract

The invention discloses a liquid transfer device for gene detection, which comprises a liquid transfer platform and a liquid transfer device, wherein a plurality of reagent containers are placed on the liquid transfer platform, and the reagent containers are used for placing liquid to be configured. The liquid-transfering device hangs to be located move liquid platform's top to can be a plurality of move between the reagent container, liquid-transfering device includes installing support, single flux syringe pump and multi-flux syringe pump, single flux syringe pump with multi-flux syringe pump all is fixed in on the installing support, the installing support is a plurality of move between the reagent container, just single flux syringe pump with multi-flux syringe pump all is used for shifting a plurality ofly treat configuration liquid between the reagent container. The technical scheme of the invention can reduce the detection cost of gene sequencing.

Description

Liquid transfer equipment for gene detection

Technical Field

The invention relates to the technical field of gene detection, in particular to a liquid transferring device for gene detection.

Background

With the continuous development of gene sequencing technology, gene sequencing has become an important means for clinical test detection. Prior to gene sequencing, it is necessary to perform multiple treatments such as pipetting and mixing of different types of liquids using a pipetting device, and therefore the pipetting device is an indispensable device for gene sequencing processes.

In order to meet the requirement of high-throughput sequencing, a large amount of sample solution preparation and transmission needs to be completed, so that the pipetting equipment adopted in the market at present usually adopts a multi-channel pipetting plunger pump and is matched with a multi-well plate kit so as to meet the requirement of liquid allocation of multiple bottles of reagents simultaneously. However, when a single bottle of reagent needs to be prepared for a single liquid, the pipetting device cannot be used, and a liquid preparation workstation is often additionally arranged for manual liquid preparation. This increases the cost of detection, which leads to high costs associated with gene sequencing.

Disclosure of Invention

The invention mainly aims to provide a pipetting device for gene detection, aiming at reducing the detection cost of gene sequencing.

In order to achieve the above object, the present invention provides a pipetting apparatus for gene detection, comprising:

the liquid transferring platform is provided with a plurality of reagent containers, and the reagent containers are used for placing liquid to be configured;

the liquid-transfering device, hang and locate move liquid-transfering platform's top to can be a plurality of move between the reagent container, liquid-transfering device includes installing support, single flux syringe pump and multi-flux syringe pump, single flux syringe pump with multi-flux syringe pump all is fixed in on the installing support, the installing support is in a plurality of move between the reagent container, just single flux syringe pump with multi-flux syringe pump all is used for shifting a plurality ofly wait to dispose liquid between the reagent container.

Optionally, the liquid-transfering device further comprises a driving assembly, the single-flux injection pump and the multi-flux injection pump are respectively arranged at two opposite sides of the mounting bracket, and the driving assembly drives the single-flux injection pump and the multi-flux injection pump to slide on the mounting bracket in a reciprocating manner.

Optionally, slidable mounting has the sliding seat on the installing support, single flux syringe pump with many flux syringe pumps all install in on the sliding seat, the drive assembly drive the sliding seat slides, just the sliding seat with be provided with sliding guide between the installing support, for the sliding guide of sliding seat.

Optionally, the single-flux injection pump includes a first pump body, a first plunger, and a first gun head, a first injection cavity is provided in the first pump body, the first plunger is partially inserted into one end of the first injection cavity and can slide in the first injection cavity, and the first gun head is partially inserted into the other end of the first injection cavity to suck or discharge the liquid to be configured;

the multi-flux injection pump comprises a second pump body, a plurality of second plungers and a plurality of second gun heads, wherein a plurality of second injection cavities are formed in the second pump body, the second plungers are partially inserted into one ends of the second injection cavities and can slide in the second injection cavities, and the second gun heads are partially inserted into the other ends of the second injection cavities to suck or discharge liquid to be configured.

Optionally, sealing elements are sleeved between the first plunger and the cavity wall at the opening of the first injection cavity and between the second plunger and the cavity wall at the opening of the second injection cavity; and/or the presence of a gas in the atmosphere,

the first gun head and the first injection cavity as well as the second gun head and the second injection cavity are in interference fit.

Optionally, the single-flux injection pump further comprises a first driving motor and a first plunger supporting plate, one end of the first plunger, which is far away from the first injection cavity, is connected to the first plunger supporting plate, and the first driving motor drives the first plunger to slide back and forth through the first plunger supporting plate;

the multi-flux injection pump further comprises a second driving motor and a second plunger supporting plate, one end, far away from the second injection cavity, of the second plunger is connected to the second plunger supporting plate, and the second driving motor drives the second plunger to slide in a reciprocating mode through the second plunger supporting plate.

Optionally, the single-flux injection pump further comprises a first lance withdrawal assembly, and the first lance withdrawal assembly is used for pulling the first lance head out of the first injection cavity;

the multi-throughput injection pump further comprises a second gun withdrawing assembly, and the second gun withdrawing assembly is used for pulling the second gun head out of the second injection cavity.

Optionally, the first gun withdrawing assembly comprises a first gun withdrawing plate and a first gun withdrawing rod which are connected with each other, the first gun withdrawing rod penetrates through the first pump body, a first gun withdrawing portion is arranged on the first plunger supporting plate, the first gun withdrawing portion pushes the first gun withdrawing assembly to move, the first gun withdrawing plate is sleeved on the first gun head, a first blocking piece is fixed at one end, away from the first pump body, of the first gun head, and the first blocking piece is used for blocking the first gun head from being separated from the first gun withdrawing plate when the gun is withdrawn;

the second gun withdrawing assembly comprises a second gun withdrawing plate and a second gun withdrawing rod which are connected with each other, the second gun withdrawing rod penetrates through the second pump body, a second gun withdrawing portion is arranged on the second plunger supporting plate, the second gun withdrawing portion pushes the second gun withdrawing assembly to move, the second gun withdrawing plate is sleeved with the second gun head, a second blocking piece is fixed at one end, deviating from the second pump body, of the second gun head, and the second blocking piece is used for blocking the second gun head to be separated from the second gun withdrawing plate when the gun is withdrawn.

Optionally, the first gun head comprises a first inner gun head and a first outer gun head, the first outer gun head is inserted into the first installation cavity, the first inner gun head is partially sleeved in the first outer gun head, and a first capacitance cavity is formed between the first inner gun head and the first outer gun head so as to form capacitance for monitoring the distance from the first gun head to the liquid level of the liquid to be configured;

the second gun head comprises a second inner gun head and a second outer gun head, the second outer gun head is inserted into the second mounting cavity, the second inner gun head is partially sleeved in the second outer gun head, and a second capacitance cavity is formed between the second inner gun head and the second outer gun head so as to form capacitance for monitoring the distance from the second gun head to the liquid level of the liquid to be configured.

Optionally, a pressure sensor is disposed in the first mounting cavity and/or the second mounting cavity to detect a pressure change in the first mounting cavity and/or the second mounting cavity.

According to the technical scheme, the liquid transfer platform is arranged, a plurality of reagent containers are placed on the liquid transfer platform, and the reagent containers are used for placing liquid to be configured. The liquid-transfering device is suspended above the liquid-transfering platform and can be moved between several reagent containers, and said liquid-transfering device includes mounting support, single-flux injection pump and multi-flux injection pump, on said mounting support a single-flux injection pump is fixed for meeting single-bottle reagent individual liquid configuration, and a multi-flux injection pump is fixed for meeting multi-bottle reagent simultaneous liquid configuration. And the single-flux injection pump and the multi-flux injection pump are used for transferring the liquid to be configured among the plurality of reagent containers. Therefore, the liquid transfer equipment can meet the requirement of liquid configuration of a single bottle of reagent and the requirement of liquid configuration of multiple bottles of reagents simultaneously, and the detection cost of gene sequencing is reduced.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the structures shown in the drawings without creative efforts.

FIG. 1 is a schematic view of the structure of a pipetting device for gene detection according to an embodiment of the present invention;

FIG. 2 is a schematic view of the structure of the pipetting device in the pipetting arrangement of FIG. 1;

FIG. 3 is a schematic view of the mounting bracket of the pipetting device of FIG. 2;

FIG. 4 is a schematic view of the construction of a single-throughput syringe pump in the pipetting device of FIG. 2;

FIG. 5 is a side cross-sectional view of the single-pass syringe pump of FIG. 4;

FIG. 6 is a schematic view of the construction of a single-throughput syringe pump in the pipetting device of FIG. 2;

FIG. 7 is a side cross-sectional view of the single-pass syringe pump of FIG. 6;

fig. 8 is a cross-sectional view of the first lance tip in the single-throughput syringe pump of fig. 4.

The reference numbers illustrate:

the implementation, functional features and advantages of the present invention will be further described with reference to the accompanying drawings.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

It should be noted that, if directional indications (such as up, down, left, right, front, back, 8230; etc.) are involved in the embodiment of the present invention, the directional indications are only used for explaining the relative positional relationship between the components, the motion situation, etc. in a specific posture (as shown in the figure), and if the specific posture is changed, the directional indications are correspondingly changed.

In addition, if there is a description of "first", "second", etc. in an embodiment of the present invention, the description of "first", "second", etc. is for descriptive purposes only and is not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In addition, the meaning of "and/or" appearing throughout is to include three juxtapositions, exemplified by "A and/or B," including either the A or B arrangement, or both A and B satisfied arrangement. In addition, technical solutions between the embodiments may be combined with each other, but must be based on the realization of the technical solutions by a person skilled in the art, and when the technical solutions are contradictory to each other or cannot be realized, such a combination should not be considered to exist, and is not within the protection scope of the present invention.

The present invention provides a pipetting apparatus 100 for gene detection.

In the embodiment of the present invention, as shown in fig. 1 to fig. 2, the pipetting apparatus 100 for gene detection comprises a pipetting platform 110 and a pipetting device 120, wherein a plurality of reagent containers 111 are placed on the pipetting platform 110, and the reagent containers 111 are used for placing the liquid to be configured. The pipetting device 120 is suspended above the pipetting platform 110 and can move among the plurality of reagent containers 111, the pipetting device 120 comprises a mounting bracket 130, a single-flux injection pump 140 and a multi-flux injection pump 150, the single-flux injection pump 140 and the multi-flux injection pump 150 are both fixed on the mounting bracket 130, the mounting bracket 130 moves among the plurality of reagent containers 111, and the single-flux injection pump 140 and the multi-flux injection pump 150 are both used for transferring liquid to be configured among the plurality of reagent containers 111.

Specifically, the pipetting platform 110 is used as a main liquid configuration platform, and the plurality of reagent containers 111 and the first and second tips 143 and 153 to be replaced are all placed on the pipetting platform 110, so as to facilitate the configuration of the liquid to be configured and the replacement of the first and second tips 143 and 153. The reagent container 111 includes a plurality of multi-well plate containers, a plurality of reagent bottles, and the like. The multiple multi-well plate containers and the multiple reagent bottles are arranged on the liquid transfer platform 110 in a preset mode, and the liquid transfer device 120 is suspended above the liquid transfer platform 110 and can move among the multiple reagent containers 111, so that the liquid to be configured in different reagent containers 111 is transferred, and finally the required solution to be detected is obtained. The liquid-transferring device 120 is suspended above the liquid-transferring platform 110 by a mounting frame, and at least one guide rail and a driving device are preset on the mounting frame, and the driving device drives the liquid-transferring device 120 to move on the guide rail, thereby completing the transfer of the liquid to be configured. Generally, the single-throughput syringe pump 140 is used with a reagent bottle or a reagent kit, while the multi-throughput syringe pump 150 is used with a predetermined multi-well plate container, which cannot be interchanged with each other. This results in that the pipetting device 100 on the market today often only allows multiple bottles of reagents to be liquid-dispensed simultaneously. However, when a single bottle of reagent needs to be dispensed, the pipetting device 100 cannot be used, and an additional dispensing station is often needed for manual dispensing. Thus greatly increasing the cost of reagent preparation and further increasing the cost of gene detection. The mounting bracket 130 is fixed with a single-volume syringe pump 140 for satisfying the single liquid configuration of a single bottle of reagent, and is fixed with a multi-volume syringe pump 150 for satisfying the liquid configuration of a plurality of bottles of reagent simultaneously. Therefore, the liquid configuration of a single bottle of reagent and the liquid configuration of a plurality of bottles of reagent are simultaneously satisfied by the liquid transferring device 100, and the detection cost of gene sequencing is reduced. And optical couplers are mounted between the mounting bracket 130 and the single-flux syringe pump 140 and/or between the mounting bracket 130 and the multi-flux syringe pump 150, so as to monitor the movement position of the single-flux syringe pump 140 relative to the mounting bracket 130 and/or the multi-flux syringe pump 150 relative to the mounting bracket 130.

According to the technical scheme, the liquid transfer platform 110 is arranged, a plurality of reagent containers 111 are placed on the liquid transfer platform 110, and the reagent containers 111 are used for placing liquid to be configured. The pipetting device 120 is suspended above the pipetting platform 110 and can move among a plurality of reagent containers 111, the pipetting device 120 comprises a mounting bracket 130, a single-flux injection pump 140 and a multi-flux injection pump 150, and the mounting bracket 130 is fixed with the single-flux injection pump 140 for satisfying the single-bottle reagent independent liquid configuration and the multi-flux injection pump 150 for satisfying the multi-bottle reagent simultaneous liquid configuration. And the single-and multi-throughput syringe pumps 140, 150 are each used to transfer liquid to be configured between the plurality of reagent containers 111. Therefore, the pipetting device 100 can simultaneously meet the liquid configuration of a single bottle of reagent and the liquid configuration of a plurality of bottles of reagent, thereby reducing the detection cost of gene sequencing.

Referring to fig. 1 to 3, in an embodiment, the pipetting device 120 further includes a driving assembly 131, the single-throughput syringe pump 140 and the multi-throughput syringe pump 150 are respectively disposed at two opposite sides of the mounting bracket 130, and the driving assembly 131 drives the single-throughput syringe pump 140 and the multi-throughput syringe pump 150 to slide reciprocally on the mounting bracket 130. Specifically, the pipetting device 120 is driven by the driving device to move horizontally above the pipetting platform 110, so that the mounting bracket 130 drives the single-flux injection pump 140 and the multi-flux injection pump 150 to move horizontally, and the driving assembly 131 can drive the single-flux injection pump 140 and the multi-flux injection pump 150 to move vertically relatively, so that the single-flux injection pump 140 and the multi-flux injection pump 150 complete the transfer of the liquid to be configured. And the single-and multi-throughput syringes 140 and 150 are separately disposed at opposite sides of the mounting bracket 130 so that the movements thereof do not interfere with each other. Of course, in other embodiments, the single-and multi-port syringe pumps 140, 150 may be arranged side-by-side, or the single-and multi-port syringe pumps 140, 150 may be angled.

Further, a sliding seat 132 is slidably mounted on the mounting bracket 130, the single-throughput syringe pump 140 and the multi-throughput syringe pump 150 are both mounted on the sliding seat 132, the driving assembly 131 drives the sliding seat 132 to slide, and a sliding guide 133 is disposed between the sliding seat 132 and the mounting bracket 130 to guide the sliding of the sliding seat 132. Specifically, the driving assembly 131 may drive the sliding seat 132 to slide vertically, and the single-throughput syringe pump 140 and the multi-throughput syringe pump 150 are both installed on the sliding seat 132, so that the sliding seat 132 drives the single-throughput syringe pump 140 and the multi-throughput syringe pump 150 to move. In the actual working process, the liquid configuration of a single bottle of reagent and the liquid configuration of multiple bottles of reagent can not be performed simultaneously, that is, the single-throughput syringe pump 140 and the multi-throughput syringe pump 150 can not work simultaneously, so that the requirement can be met by driving the single-throughput syringe pump 140 and the multi-throughput syringe pump 150 to move by the same driving assembly 131, and the method is more economical. In another embodiment, two sets of drive assemblies 131 may be used to drive the movement of the single-and multi-syringe pumps 140, 150, respectively. At this time, two sliding seats 132 are correspondingly provided, which respectively drive the single-throughput syringe pump 140 and the multi-throughput syringe pump 150 to move independently. And in order to ensure the movement fluency of the sliding seat 132, a sliding guide rail 133 is provided between the sliding seat 132 and the mounting bracket 130, thereby guiding the sliding of the sliding seat 132.

In an embodiment, referring to fig. 4 and 5 in combination, the single-throughput syringe pump 140 comprises a first pump body 141, a first plunger 142 and a first gun head 143, wherein a first injection cavity 144 is arranged in the first pump body 141, the first plunger 142 is partially inserted into one end of the first injection cavity 144 and can slide in the first injection cavity 144, and the first gun head 143 is partially inserted into the other end of the first injection cavity 144 to suck or discharge a liquid to be dispensed. Specifically, a first injection cavity 144 is formed in the first pump body 141 for storing the liquid to be configured, the first plunger 142 is inserted from one end far away from the reagent container 111, the first lance tip 143 is partially inserted into the other end of the first injection cavity 144, and the first plunger 142 moves in the first injection cavity 144 through the end inserted into the first injection cavity 144, so as to change the pressure in the first injection cavity 144, and the first lance tip 143 sucks or discharges the liquid to be configured.

And in order to increase the sealing performance of the first injection cavity 144, a sealing member 145 is sleeved between the first plunger 142 and the cavity wall at the opening of the first injection cavity 144.

In another embodiment, first lance tip 143 is in an interference fit with first injection cavity 144. Specifically, here, the first lance tip 143 is slightly interference-fitted with the first injection cavity 144, so that the first lance tip 143 can be clamped in the first injection cavity 144 to improve the sealing performance of the first injection cavity 144. And facilitates withdrawal of first lance tip 143 from first injection chamber 144, thereby facilitating replacement of first lance tip 143.

With combined reference to fig. 6 and 7, in an embodiment, the single-flux injection pump 140 includes a second pump body 151, a plurality of second plungers 152 and a plurality of second gun heads 153, wherein the second pump body 151 is provided with a plurality of second injection cavities 154, the second plungers 152 are partially inserted into one ends of the second injection cavities 154 and can slide in the second injection cavities 154, and the second gun heads 153 are partially inserted into the other ends of the second injection cavities 154 to suck or discharge the liquid to be prepared. Specifically, a second injection cavity 154 is formed in the second pump body 151 and used for storing the liquid to be prepared, the second plunger 152 is inserted from one end far away from the reagent container 111, the second gun head 153 is partially inserted into the other end of the second injection cavity 154, and the pressure in the second injection cavity 154 is changed through the movement of one end of the second plunger 152 inserted into the second injection cavity 154 in the second injection cavity 154, so that the second gun head 153 sucks or discharges the liquid to be prepared. In this embodiment, 8 second injection cavities 154 are provided in the second pump body 151, and the number of holes of the multi-plate hole container used therein should also be a multiple of 8. Of course, in other embodiments, the number of the second injection cavities 154 in the second pump body 151 may also be greater than 8, or less than or equal to 8, and greater than or equal to 2, and the number of holes of the multi-plate hole container used is correspondingly set.

And in order to increase the sealing performance of the second injection cavity 154, a sealing element 145 is sleeved between the second plunger 152 and the cavity wall at the opening of the second injection cavity 154.

In another embodiment, the second lance tip 153 is in an interference fit with the second injection cavity 154. Specifically, the second lance tip 153 is slightly in interference fit with the second injection cavity 154, so that the second lance tip 153 can be clamped in the second injection cavity 154 to improve the tightness of the second injection cavity 154. And facilitates the withdrawal of the second lance tip 153 from the second injection chamber 154, thereby facilitating replacement of the second lance tip 153.

Referring again to fig. 4 and 5 in combination, in one embodiment, the single-throughput syringe pump 140 further includes a first drive motor 146 and a first plunger support plate 147, an end of the first plunger 142 remote from the first syringe chamber 144 is connected to the first plunger support plate 147, and the first drive motor 146 drives the first plunger 142 to slide back and forth via the first plunger support plate 147. Specifically, the first driving motor 146 drives the first plunger 142 to move through the first plunger supporting plate 147, in this embodiment, the first driving motor 146 drives the first plunger supporting plate 147 to move through a screw, and one end of the first plunger 142, which is far away from the first injection cavity 144, is connected to the first plunger supporting plate 147, so that the first plunger supporting plate 147 drives the first plunger 142 to move, thereby achieving liquid suction and liquid spitting.

Referring again to fig. 6 and 7 in combination, the multi-throughput syringe pump 150 further includes a second drive motor 155 and a second plunger plate 156, an end of the second plunger 152 distal from the second injection chamber 154 is attached to the second plunger plate 156, and the second drive motor 155 drives the second plunger 152 to slide back and forth via the second plunger plate 156. Specifically, the second driving motor 155 drives the second plunger 152 to move through the second plunger plate 156, in this embodiment, the second driving motor 155 drives the second plunger plate 156 to move through a screw, and one end of the second plunger 152 far away from the second injection cavity 154 is connected to the second plunger plate 156, so that the second plunger plate 156 drives the second plunger 152 to move, thereby sucking liquid and spitting liquid.

To reduce the occurrence of reagent contamination, the single-flux injection pump 140 needs to replace the first lance tip 143 after completing one reagent transfer, and to facilitate the replacement of the first lance tip 143, in an embodiment, referring to fig. 4 and 5 in combination, the single-flux injection pump 140 further includes a first lance withdrawal assembly 160, and the first lance withdrawal assembly 160 is configured to withdraw the first lance tip 143 from the first injection cavity 144. Therefore, the first gun head 143 of the single-flux injection pump 140 is replaced, the first gun head 143 of the pipetting device 100 is automatically replaced, and the automation degree of the pipetting device 100 is improved.

Further, the first gun withdrawing assembly 160 includes a first gun withdrawing plate 161 and a first gun withdrawing rod 162 that are connected to each other, the first gun withdrawing rod 162 penetrates through the first pump body 141, a first gun withdrawing portion 147a is disposed on the first plunger supporting plate 147, the first gun withdrawing portion 147a pushes the first gun withdrawing assembly 160 to move, the first gun withdrawing rod 162 is sleeved on the first gun head 143, a first blocking member is fixed to one end of the first gun head 143 that deviates from the first pump body 141, and the first blocking member is used for blocking the first gun head 143 from deviating from the first gun withdrawing plate 161 when the gun is withdrawn. Specifically, the first gun withdrawing plate 161 is sleeved on the first gun head 143, and the first gun withdrawing rod 162 is connected to the first gun withdrawing plate 161 and disposed at an included angle. The first pump body 141 is provided with a through hole, and one end of the first gun withdrawing rod 162 far away from the first gun withdrawing plate 161 passes through the through hole and partially protrudes out of the through hole. Since the first plunger supporting plate 147 moves up and down and has the first gun retreating portion 147a, the first gun retreating portion 147a is located above the first gun retreating rod 162. When the first plunger supporting plate 147 moves to a certain position, the first gun withdrawing portion 147a abuts against the first gun withdrawing rod 162 and pushes the first gun withdrawing rod 162 to move, so as to drive the first gun withdrawing plate 161 to withdraw the gun. One end of the first lance head 143 departing from the first pump body 141 is fixed with a first blocking member, and the first blocking member abuts against one side of the first lance withdrawing plate 161 departing from the first pump body 141, so that the first lance head 143 is prevented from being withdrawn from the first lance withdrawing plate 161 when the lance is withdrawn. So that the first gun withdrawing plate 161 pulls the first gun head 143 out of the first injection cavity 144. And placed on the pipetting platform 110. Then, the pipetting device 120 is moved to the position of the first tip 143 to be replaced, the first tip 143 which is not contaminated is replaced, and then the next pipetting is performed. Wherein, the first gun moving plate 161 and the first gun moving rod 162 can be integrally formed, or fastened and connected by screw connection, clamping, bonding or riveting. And for improving the stability when moving back the rifle, first move back rifle bar 162 has two to locate the relative both ends of first moving back rifle plate 161 separately.

To reduce the occurrence of reagent contamination, the multi-flow syringe pump 150 needs to replace the second lance tip 153 after completing a reagent transfer, and to facilitate replacement of the second lance tip 153, in an embodiment, referring to fig. 6 and 7 in combination, the multi-flow syringe pump 150 further includes a second lance retracting assembly 170, and the second lance retracting assembly 170 is used for pulling the second lance tip 153 out of the second injection cavity 154. Therefore, the second gun head 153 of the single-flux injection pump 140 can be replaced, the second gun head 153 of the pipetting device 100 can be automatically replaced, and the automation degree of the device can be improved.

Further, the second gun withdrawing assembly 170 includes a second gun withdrawing plate 171 and a second gun withdrawing rod 172 which are connected to each other, the second gun withdrawing rod 172 penetrates through the second pump body 151, a second gun withdrawing portion 156a is arranged on the second plunger supporting plate 156, the second gun withdrawing portion 156a pushes the second gun withdrawing assembly 170 to move, the second gun withdrawing rod 172 is sleeved on the second gun head 153, a second blocking member is fixed to one end, deviating from the second pump body 151, of the second gun head 153, and the second blocking member is used for blocking the second gun head 153 to be separated from the second gun withdrawing plate 171 when the gun is withdrawn. Specifically, the second gun head 153 is sleeved with the second gun withdrawing plate 171, and the second gun withdrawing rod 172 is connected to the second gun withdrawing plate 171 and disposed at an included angle. A through hole is formed in the second pump body 151, and one end of the second gun backing rod 172, which is far away from the second gun backing plate 171, passes through the through hole and partially protrudes out of the through hole. Because the second plunger support 156 moves up and down and has a second gun withdrawing portion 156a, the second gun withdrawing portion 156a is located above the second gun withdrawing rod 172. When the second plunger supporting plate 156 moves to a certain position, the second gun withdrawing portion 156a abuts against the second gun withdrawing rod 172, and pushes the second gun withdrawing rod 172 to move, so as to drive the second gun withdrawing plate 171 to withdraw the gun. One end of the second gun head 153, which is away from the second pump body 151, is fixed with a second blocking member, and the second blocking member abuts against one side of the second gun withdrawing plate 171, which is away from the second pump body 151, so that the second gun head 153 is prevented from being withdrawn from the second gun withdrawing plate 171. So that the second gun withdrawing plate 171 pulls the second gun head 153 out of the second injection cavity 154. And placed on the pipetting platform 110. Then, the pipetting device 120 is moved to the second tip 153 to be replaced, the second tip 153 is replaced without contamination, and then next pipetting is performed. The second gun withdrawing plate 171 and the second gun withdrawing rod 172 can be integrally formed or fastened and connected by threads, clamping, bonding, riveting or the like. And for improving the stability when withdrawing the gun, the second withdrawing rod 172 has two and is separately disposed at the opposite ends of the second withdrawing plate 171.

In order to monitor the distance from the first lance tip 143 to the liquid level of the liquid to be configured, in an embodiment, referring to fig. 4, fig. 5 and fig. 8 in combination, the first lance tip 143 includes a first inner lance tip 143a and a first outer lance tip 143b, the first outer lance tip 143b is inserted into the first mounting cavity, the first inner lance tip 143a is partially sleeved in the first outer lance tip 143b, and a first capacitance cavity 143c is formed between the first inner lance tip 143a and the first outer lance tip 143b, so as to form a capacitance monitoring distance from the first lance tip 143 to the liquid level of the liquid to be configured. Specifically, the first outer bayonet 143b is inserted into the first mounting cavity, and the first inner bayonet 143a is partially sleeved in the first outer bayonet 143b, so that the two are spaced in the radial direction, abut against each other in the axial direction, and are separated by the spacer 143 e. Thereby forming a first capacitance chamber 143c, when the first inner gun head 143a contacts liquid, the capacitance in the first gun head 143 changes, thereby transmitting a signal to the control system, and enabling the first plunger 142 to move for liquid extraction. In addition, in order to increase the capacitance in the first capacitor chamber 143c, the change in capacitance in the first lance tip 143 is amplified, so that the first capacitor chamber 143c is filled with the filler 143d. In order to reduce the loss of the outer wall of the first gun head 143, the liquid is disposed, so in one embodiment, the single-pass injection pump 140 moves downward with the liquid falling to follow the liquid surface when absorbing the liquid.

In order to monitor the distance from the second lance tip 153 to the liquid level of the liquid to be configured, in an embodiment, the second lance tip 153 includes a second inner lance head 153a and a second outer lance head 153b, the second outer lance head 153b is inserted into the second installation cavity, the second inner lance head 153a is partially sleeved in the second outer lance head 153b, and a second capacitance cavity 153c is formed between the second inner lance head 153a and the second outer lance head 153b, so as to form a capacitance monitoring distance from the second lance head 153 to the liquid level of the liquid to be configured. Specifically, the second outer lance head 153b is inserted into the second mounting cavity, and the second inner lance head 153a is partially sleeved in the second outer lance head 153b, so that the two are spaced in the radial direction, abutted in the axial direction, and separated by the spacer 143 e. Thereby forming a second capacitance chamber 153c, when the second inner gun head 153a contacts liquid, the capacitance in the second gun head 153 changes, thereby transmitting a signal to the control system, so that the second plunger 152 moves to draw liquid. In addition, in order to increase the capacitance in the second capacitor chamber 153c, the change in capacitance in the second lance tip 153 is amplified, so that the second capacitor chamber 153c is filled with the filler 143d.

In one embodiment, a pressure sensor 148 is disposed within each of the first and/or second mounting cavities to detect pressure changes within the first and/or second mounting cavities. Specifically, the pressure sensor 148 can effectively monitor the pressure change in the first installation cavity and/or the second installation cavity, and judge whether the first installation cavity and/or the second installation cavity leaks liquid in the liquid transferring process according to the pressure change, so that the pressure sensor 148 is installed on the inner wall of the first installation cavity and/or the second installation cavity.

The operation of the single-flux injection pump 140 in the pipetting apparatus 100 for gene detection is as follows:

the driving mechanism drives the pipetting device 100 to move above the first gun head 143, then the driving assembly 131 drives the single-flux injection pump 140 to move downwards relative to the mounting bracket 130, the first gun head 143 is inserted into the first injection cavity 144, so that the first gun head 143 is in slight interference fit with the first injection cavity 144, then the driving assembly 131 drives the single-flux injection pump 140 to move upwards relative to the mounting bracket 130, the driving mechanism drives the pipetting device 100 to move above the liquid to be configured, the driving assembly 131 drives the single-flux injection pump 140 to move downwards relative to the mounting bracket 130, and the liquid level detector is started until the single-flux injection pump is contacted with the liquid to be configured, the capacitance in the first gun head 143 changes, and after the liquid level detector detects, the first gun head 143 sucks the liquid to be configured, and the first gun head 143 moves downwards along with the descending of the liquid in the pipetting process to follow the liquid level. After the pipetting is completed, the driving assembly 131 drives the single-throughput syringe pump 140 to move upward relative to the mounting bracket 130, and the pipetting device 100 is driven by the driving mechanism to move to the upper part of another reagent container 111 for solution preparation. Then the driving mechanism drives the pipetting apparatus 100 to move to the preset position, so that the first driving motor 146 drives the first plunger supporting plate 147 to move, and then the first gun withdrawing portion 147a on the first plunger supporting plate 147 pushes the first gun withdrawing rod 162 to move, so that the first gun withdrawing plate 161 withdraws the first gun head 143 from the first injection cavity 144. The above steps are then repeated, thereby completing single throughput pipetting.

The operation of the multi-throughput syringe pump 150 in the pipetting apparatus 100 for gene detection is as follows:

the driving mechanism drives the pipetting device 100 to move above the second pipette tip 153, then the driving assembly 131 drives the single-flux injection pump 140 to move downwards relative to the mounting bracket 130, the second pipette tip 153 is inserted into the second injection cavity 154, so that the second pipette tip 153 is in slight interference fit with the second injection cavity 154, then the driving assembly 131 drives the multi-flux injection pump 150 to move upwards relative to the mounting bracket 130, the driving mechanism drives the pipetting device 100 to move above the liquid to be prepared, the driving assembly 131 drives the multi-flux injection pump 150 to move downwards relative to the mounting bracket 130 until the multi-flux injection pump is in contact with the liquid to be prepared, the second pipette tip 153 sucks the liquid to be prepared, and after the sucking is completed, the driving assembly 131 drives the single-flux injection pump 140 to move upwards relative to the mounting bracket 130, and the driving mechanism drives the pipetting device 100 to move above another reagent container 111 for solution preparation. The drive mechanism then drives the pipetting device 100 to move to the preset position, so that the second drive motor 155 drives the second plunger plate 156 to move, and then the second ejection portion 156a on the second plunger plate 156 pushes the second ejection rod 172 to move, so that the second ejection plate 171 ejects the second gun head 153 from the second injection cavity 154. The above steps are then repeated, thereby completing the multi-throughput pipetting.

The above description is only a preferred embodiment of the present invention, and is not intended to limit the scope of the present invention, and all equivalent structural changes made by using the contents of the present specification and the drawings, or any other related technical fields directly/indirectly applied to the present invention are included in the scope of the present invention.

Claims (10)

1. A pipetting apparatus for gene testing, comprising:

the liquid transferring platform is provided with a plurality of reagent containers, and the reagent containers are used for placing liquid to be configured;

the liquid-transfering device, the suspension is located move liquid platform's top to can be a plurality of move between the reagent container, liquid-transfering device includes installing support, single flux syringe pump and many flux syringe pumps, single flux syringe pump with many flux syringe pumps all are fixed in on the installing support, the installing support is a plurality of move between the reagent container, just single flux syringe pump with many flux syringe pumps all are used for shifting a plurality ofly wait to dispose liquid between the reagent container.

2. A pipetting apparatus for gene testing as recited in claim 1 wherein the pipetting device further comprises a drive assembly, the single-throughput syringe pump and the multi-throughput syringe pump being disposed on opposite sides of the mounting bracket and the drive assembly driving the single-throughput syringe pump and the multi-throughput syringe pump to slide reciprocally on the mounting bracket.

3. The pipette device for gene detection according to claim 2, wherein a slide base is slidably mounted on the mounting bracket, the single-flux syringe pump and the multi-flux syringe pump are both mounted on the slide base, the drive assembly drives the slide base to slide, and a slide guide rail is provided between the slide base and the mounting bracket to guide the slide base to slide.

4. A pipetting device for gene detection as recited in claim 1, wherein the single-flux syringe pump comprises a first pump body, a first plunger and a first lance head, the first pump body is provided with a first injection cavity, the first plunger is partially inserted into one end of the first injection cavity and can slide in the first injection cavity, and the first lance head is partially inserted into the other end of the first injection cavity to suck or discharge the liquid to be dispensed;

the multi-flux injection pump comprises a second pump body, a plurality of second plungers and a plurality of second gun heads, wherein a plurality of second injection cavities are formed in the second pump body, the second plungers are partially inserted into one ends of the second injection cavities and can slide in the second injection cavities, and the second gun heads are partially inserted into the other ends of the second injection cavities to suck or discharge liquid to be configured.

5. A pipetting device for gene detection as recited in claim 4 wherein a seal is sleeved between the first plunger and the chamber wall at the opening of the first injection chamber and between the second plunger and the chamber wall at the opening of the second injection chamber; and/or the presence of a gas in the gas,

the first gun head and the first injection cavity as well as the second gun head and the second injection cavity are in interference fit.

6. A pipetting apparatus for gene detection as recited in claim 4 wherein the single flux syringe pump further comprises a first drive motor and a first plunger blade, an end of the first plunger remote from the first syringe chamber is connected to the first plunger blade, and the first drive motor drives the first plunger to slide reciprocally through the first plunger blade;

the multi-flux injection pump further comprises a second driving motor and a second plunger supporting plate, one end, far away from the second injection cavity, of the second plunger is connected to the second plunger supporting plate, and the second driving motor drives the second plunger to slide in a reciprocating mode through the second plunger supporting plate.

7. A pipetting apparatus as recited in claim 6 wherein the single-throughput syringe pump further comprises a first withdrawal assembly for withdrawing the first tip from the first injection chamber;

the multi-throughput injection pump further comprises a second gun withdrawing assembly, and the second gun withdrawing assembly is used for pulling the second gun head out of the second injection cavity.

8. A liquid transfer apparatus for gene detection according to claim 7, wherein the first gun backing assembly comprises a first gun backing plate and a first gun backing rod which are connected with each other, the first gun backing rod penetrates through the first pump body, a first gun backing part is arranged on the first plunger support plate, the first gun backing part pushes the first gun backing assembly to move, the first gun backing plate is sleeved on the first gun head, a first blocking part is fixed at one end of the first gun head, which is far away from the first pump body, and the first blocking part is used for blocking the first gun head from being pulled out of the first gun backing plate when the gun is backed;

the second gun withdrawing assembly comprises a second gun withdrawing plate and a second gun withdrawing rod which are connected with each other, the second gun withdrawing rod penetrates through the second pump body, a second gun withdrawing portion is arranged on the second plunger supporting plate, the second gun withdrawing portion pushes the second gun withdrawing assembly to move, the second gun withdrawing plate is sleeved with the second gun head, a second blocking piece is fixed at one end, deviating from the second pump body, of the second gun head, and the second blocking piece is used for blocking the second gun head to be separated from the second gun withdrawing plate when the gun is withdrawn.

9. The pipetting device for gene detection according to claim 4, wherein the first gun head comprises a first inner gun head and a first outer gun head, the first outer gun head is inserted into the first mounting cavity, the first inner gun head is partially sleeved in the first outer gun head, and a first capacitance cavity is formed between the first inner gun head and the first outer gun head so as to form capacitance for monitoring the distance from the first gun head to the liquid level of the liquid to be configured;

the second gun head comprises a second inner gun head and a second outer gun head, the second outer gun head is inserted into the second mounting cavity, the second inner gun head is partially sleeved in the second outer gun head, and a second capacitance cavity is formed between the second inner gun head and the second outer gun head so as to form capacitance for monitoring the distance from the second gun head to the liquid level of the liquid to be configured.

10. Pipetting device for gene detection according to claim 4, characterized in that a pressure sensor is provided in the first mounting chamber and/or the second mounting chamber to detect pressure changes in the first mounting chamber and/or the second mounting chamber.

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