CN101441223B - Precision trace sampling apparatus with mixing function - Google Patents

Abstract

The invention relates to an automatic sampling device for a biochemical analysis instrument, in particular to a precision micro-sampling device with a mixing function integrating sampling and sample and reagent mixing functions. It includes a sealed cylinder body and a sampling needle tube and a linear motor arranged at the bottom of the cylinder body to communicate with the inner cavity of the cylinder body; the inner cavity of the sealed cylinder body is a stepped cavity with a constricted lower part. A mixing piston with an inner cavity and a sampling plunger rod is provided; a linear motor drives the sampling plunger rod and the mixing piston to reciprocate, so as to realize the precise sampling of trace samples and the mixing function of samples and reagents. The invention overcomes the defects of complex layout and cumbersome structure of the automatic biochemical analyzer caused by the separation of the sampling device and the mixing device in the existing biochemical analyzer, which are not conducive to the integration and miniaturization of the biochemical analyzer.

Description

Precision micro-sampling device with mixing function

technical field

The invention relates to an automatic sampling and sample and reagent mixing device for biochemical analysis instruments, in particular to a precision micro-sampling device integrating the functions of sampling and sample and reagent mixing.

Background technique

In the automatic biochemical analyzer, in order to improve the accuracy of the test results, both the sampling device and the mixing device are necessary. The main function of the sampling device is to absorb and release samples or reagents; the main function of the mixing device is to stir and mix the samples or reagents released into the reaction cup to ensure the uniformity of the reaction and the accuracy of the test results.

At present, the sampling and mixing methods in the existing automatic biochemical analyzers are basically similar, that is, the sampling device and the mixing device are separated, and two independent systems are used to control the operation process separately without interfering with each other. The sampling device and the mixing device are respectively installed on the automatic biochemical analyzer, surrounded by sample trays, reagent trays and reaction trays. Driven by the motor, the sampling device drives the cantilever of the sampling needle, and puts the samples and reagents in the reaction tray in turn. Afterwards, driven by the motor, the mixing device drives the cantilever of the stirring needle, so that the stirring needle is stirred in the cuvette.

Since the sampling device and the mixing device are separated, the layout of the automatic biochemical analyzer is not simple enough, especially for a relatively large biochemical analyzer, the more parts, the higher the cost, and the greater the probability of failure.

Contents of the invention

The purpose of the present invention is to provide a precision micro-sampling device with a mixing function, so as to solve the problem of the separation of the sampling device and the mixing device in the existing biochemical analyzer, which causes the layout of the fully automatic biochemical analyzer to be complicated, the structure is cumbersome, and it is not conducive to biochemical analysis. The integration and miniaturization of the instrument and other defects.

The precision micro-sampling device with mixing function of the present invention includes a sealed cylinder, a sampling needle tube and a linear motor arranged at the bottom of the cylinder and communicating with the inner cavity of the cylinder; the inner cavity of the sealed cylinder is the lower part There is a stepped cavity with a constricted neck, and a cylindrical surface is provided in the inner cavity of the sealed cylinder for sliding fit with the lower constricted cavity of the sealed cylinder. The upper end of the mixed piston has a boss, and the mixed piston also has a sealing cylinder The inner cavity coaxial with the body, and a sampling plunger rod that is slippery with it is provided in the inner cavity; a sleeve is fixed at the upper end of the mixing piston;

A shaft with a flange at the lower end axially penetrates the inner cavity of the sealed cylinder and the sleeve, its lower end is connected to the sampling plunger rod, and its upper end is connected to the output shaft of the linear motor; The linear motor is connected to the gland at the upper end of the sealed cylinder through a motor bracket;

An upper permanent magnet is arranged on the lower end surface of the gland at the upper end of the sealing cylinder body, and a lower permanent magnet is arranged on the upper end surface of the sleeve.

A photoelectric sensor is also provided on the motor bracket, and a corresponding position pointer is provided on the shaft to realize precise quantitative sampling.

The present invention works in two situations. One is when the sample is injected, the output shaft of the linear motor drives the sampling plunger rod to move upward through the shaft rod, and at the same time the mixing piston is brought to the highest position, and the two permanent magnets attract each other, and the sampling plunger rod can When moving downward, the mixing piston is held by the magnet on its upper end and fixed, so accurate sampling can be carried out within a certain range; secondly, when mixing, the linear motor can drive the mixing piston to move within a certain range through the shaft. Able to aspirate and dispense large volumes of reagents for mixing.

The precision micro-sampling device with mixing function of the present invention integrates the sampling device and the mixing device, simplifies the structure of the automatic biochemical analyzer, is simple and convenient to operate, and is accurate in quantification, and satisfies the high precision and miniaturization of the automatic biochemical analysis system need.

Description of drawings

Fig. 1 is the structural representation of device of the present invention;

Fig. 2 is a schematic diagram of the working state of the device of the present invention.

Detailed ways

The device of the present invention will be further described in detail in the following embodiments given in conjunction with the accompanying drawings.

Referring to Fig. 1, the precise micro-sampling device with mixing function comprises a sealed cylinder 11 and a sampling needle tube 13 and a linear motor 1 which are arranged on the bottom of the cylinder and communicate with the cylinder cavity; the sealed cylinder The inner cavity of 11 is a stepped cavity with a constricted lower part, and a cylindrical surface is provided in the inner cavity of the sealed cylinder 11 to slide with the lower constricted cavity of the sealed cylinder 11, and a mixing piston with a boss at its upper end 10. The mixing piston 10 also has an inner cavity coaxial with the sealed cylinder 11, and a sampling plunger rod 16 that is slippery with it is provided in the inner cavity; a sleeve 8 is fixed on the upper end of the mixing piston 10;

A shaft rod 18 with a flange at the lower end axially penetrates the inner cavity of the sealing cylinder 11 and the sleeve 8, and its lower end is connected with the sampling plunger rod 16, and its upper end is connected with the linear motor 1. The output shafts 20 are connected; the linear motor 1 is connected to the gland 5 at the upper end of the sealed cylinder 11 through the motor bracket 2;

An upper permanent magnet 6 is provided on the lower surface of the gland 5 at the upper end of the sealing cylinder 11 , and a lower permanent magnet 7 is provided on the upper surface of the sleeve 8 .

A photoelectric sensor 19 is also provided on the motor bracket 2, and a corresponding position pointer 3 is provided on the shaft 18 to realize precise quantitative sampling.

The upper end of the mixing piston 10 is connected with the sleeve 8 through a sealing block 9 .

The injection needle tube 13 is fixedly mounted on the bottom end of the sealing cylinder 11 through the injection needle tube sealing block 12 .

The inner and outer surfaces of the injection needle tube 13 are provided with Teflon coating.

The working process of the device of the present invention is as follows: during accurate micro-sampling, the output shaft 20 of the linear motor 1 drives the sampling plunger rod 16 to move upward through the shaft 18, and after moving a certain distance, the shaft 18 drives the mixing piston 10 through the sleeve 8 Continue to move upwards, when the uppermost position is reached, the upper permanent magnet 6 and the lower permanent magnet 7 attract each other. At this time, the mixing piston 10 is fixed, and then the linear motor 1 reverses to make the sampling plunger rod 16 drop for a certain distance. At this time , can absorb trace samples quantitatively, when reaching the position of spitting liquid, the motor reverses, the shaft 18 pushes the mixing piston 10 to overcome the suction force of the magnet, and at this time there is a considerable emptying volume, which can make the spitting liquid cleaner. When mixing, the linear motor 1 drives the mixing piston 10 to move within a certain range through the shaft rod 18, and can suck and discharge a considerable volume of reagents for mixing. The linear motor 1 is a stepping linear motor, and a special stepping motor controller is used to control its forward and reverse rotation.

Claims (5)

1. A precision micro-sampling device with a mixing function, comprising a sealed cylinder (11) and a sampling needle tube (13) and a linear motor (1) that are arranged on the bottom of the cylinder and communicate with the cylinder cavity, It is characterized in that: the inner cavity of the sealed cylinder (11) is a stepped cavity with a constricted lower part, and a cylindrical surface and the lower part of the sealed cylinder (11) are arranged in the inner cavity of the sealed cylinder (11). A mixing piston (10) with a boss on the upper end which is slipperily fit in the constriction cavity. The mixing piston (10) also has an inner cavity coaxial with the sealing cylinder (11), and a sliding valve is provided in the inner cavity A matched sampling plunger rod (16); a sleeve (8) is fixed at the upper end of the mixing piston (10); A shaft rod (18) with a flange at the lower end axially penetrates the inner cavity of the sealing cylinder (11) and the sleeve (8), its lower end is connected with the sampling plunger rod (16), and its upper end connected to the output shaft (20) of the linear motor (1); the linear motor (1) is connected to the gland (5) on the upper end of the sealed cylinder (11) through the motor bracket (2); An upper permanent magnet (6) is arranged on the lower surface of the gland (5) at the upper end of the sealing cylinder (11), and a lower permanent magnet (7) is arranged on the upper surface of the sleeve (8). 2. The precision micro-sampling device with mixing function according to claim 1, characterized in that, a photoelectric sensor (19) is provided on the motor bracket (2), and a photoelectric sensor (19) is arranged on the shaft rod (18) There is a position pointer (3) corresponding to it. 3. The precision micro-sampling device with mixing function according to claim 1, characterized in that, the upper end of the mixing piston (10) is connected with the sleeve (8) through a sealing briquetting block (9) . 4. The precision micro-sampling device with mixing function according to claim 1, characterized in that, the described injection needle (13) is fixedly mounted on the sealing cylinder (11) through the injection needle sealing block (12). on the bottom. 5. The precision micro-sampling device with mixing function according to claim 1, characterized in that, the inner and outer surfaces of the injection needle tube (13) are all provided with Teflon coating.

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