CN212441261U

CN212441261U - Liquid transfer device

Info

Publication number: CN212441261U
Application number: CN202021564317.5U
Authority: CN
Inventors: 刘新志; 苑宝龙
Original assignee: Joyingbio Inc
Current assignee: Joyingbio Inc
Priority date: 2020-07-31
Filing date: 2020-07-31
Publication date: 2021-02-02
Anticipated expiration: 2030-07-31

Abstract

The utility model provides a pipettor. The pipette comprises a housing, a drive, 6 injection components and a transmission. The housing has an interior cavity. The driving device is arranged outside the shell. Injection parts are disposed through the housing, wherein 6 injection parts are arranged in a 2 x 3 matrix form. At least a portion of the transmission is disposed in the internal cavity. A transmission is connected to each of the 6 injection members and the drive means to synchronize the 6 injection members for pipetting or draining. According to the utility model discloses a pipettor, through setting up reasonable mode, the pipettor can dispose on the liquid-transfering workstation of different usage, not only can be applicable to conventional target plate household utensils, can also be applicable to super miniature target plate household utensils to under the prerequisite of the rate of utilization in extravagant injection chamber, the preparation efficiency of target plate sample has been improved greatly.

Description

Liquid transfer device

Technical Field

The utility model relates to a pipettor technical field especially relates to pipettor.

Background

With the development of pipettors and related technologies in the fields of life sciences, clinical examinations, molecular medicine, biosafety, biomedicine, health and the like, detection applications at the molecular level cover various industries such as clinical diagnosis, clinical molecular diagnosis, molecular pathological diagnosis, life sciences, biological information, biosafety protection, pharmaceutical research and the like, and are widely applied to departments and units such as universities and colleges, research and development institutions, health and medical institutions, CDC (Centers for Disease Control), animal and plant quarantine, food safety, aquaculture, public security, biological anti-terrorism, medicine research and development and the like as important detection instruments.

In the detection in the related field, many pretreatment works are often required to be performed on biological materials and samples (usually in a liquid state), so that a large amount of manpower is required to perform the works such as manual pipetting, sample adding, mixing and the like, and the pretreatment works of the samples are faced with the problems of low efficiency, low repetition rate, high error rate, easy pollution to operators and the like. Therefore, there is a need for a high-throughput automated biological sample pretreatment apparatus that can perform operations such as pipetting, sample application, mixing, and the like, and prepare a large amount of samples of biological samples in a short time in a batch manner.

Because of the wide variety of target plate vessels for loading biological samples, an automated biological sample processing system that can accommodate multiple target plate vessels has become a necessary basic device in a complete system for modern life science research and biotechnology applications. The utility model discloses a pipettor is the core technical unit in the automatic processing system of biological sample to can be applied to the liquid-transfering workstation that constitutes by the combination of multichannel electronic pipettor.

To the extent that the technical unit is referred to, we can equate it to a power injector. Since the working objects of the power injector are target plate vessels which are standardized for different purposes, when the number of injection cavities of the power injector is more than 2, the center distance between the central axes of the adjacent injection cavities must meet the requirement of the basic center distance (usually 9mm) of the target plate vessels, so that the structural space between the injection cavities of the injector is extremely narrow, and various mechanical parts, motors, sensors for multiple purposes and the like are mounted in the narrow space. Based on the defects and shortcomings, the technical struggle of different manufacturers is mainly reflected in the structural form of the pipetting executing mechanism. The technical scheme in the prior art at home and abroad is mainly divided into two types according to different liquid suction driving methods of a liquid transfer device: gas-liquid mixing displacement type and gas displacement type.

The gas-liquid mixing displacement type pipettor consists of an electric industrial injection pump, an injector, a reversing valve, a pressure stabilizer, a conduit, a pipetting head, a sensor and a pipetting executing mechanism. The rear end of the conduit is connected to an injector of the electric industrial injection pump through a voltage stabilizer, a reversing valve, a pressure sensor and the like, the injector and the conduit are filled with liquid under the action of the electric industrial injection pump to form a liquid plunger with the function of the injector plunger, the front end of the conduit and parts thereof are in butt joint with the replaceable liquid transfer head, and only an air gap is reserved in the liquid transfer head to isolate the liquid plunger from sample liquid. The method has the advantages that the electric reciprocating motion injection mechanism, the pressure sensor and the like are separated, only one thin catheter enters the liquid transfer executing mechanism, the design pressure and load of the liquid transfer executing mechanism component are greatly reduced, and the requirement of the basic center distance of the target plate vessel is met. Meanwhile, the distance between the reciprocating axis of the pipetting actuating mechanism and the perpendicular line of the mechanism pivot can be minimized, and the harmful overturning moment and the fatigue deformation of the supporting component generated when the pipetting actuating mechanism is connected with the pipetting head in a pressing-in mode along the axis of the pipetting actuating mechanism. And because the separation air gap between the liquid plunger and the sample liquid is very small, the pipetting precision is high. The defects are that a gas-liquid pipeline pressure system exists, the number of components is large, debugging is complex, and maintenance cost is high. And leakage is easily generated when the room temperature is low.

The gas displacement type pipettor consists of a motor, an injector made of metal or other materials, a sensor and a pipetting executing mechanism. Typically, the reciprocating movement of the solid material plunger is performed by a motor driving a screw or timing belt through a coupling or other connecting member. That is to say, the components such as the electric reciprocating injection mechanism, the pressure sensor and the like are assembled with the liquid-transfering executing mechanism, complex pressure pipelines and pump systems are not needed, the structure hierarchy is clear, and the workload of unit structure installation and debugging is greatly reduced. The air gap for separating the solid plunger from the sample liquid depends on the yield of structural design, and the smaller the air gap is, the smaller the uncertainty range of pipetting is, and otherwise, the larger the uncertainty range is.

In view of this, the utility model provides a pipettor, it belongs to gaseous replacement formula pipettor, and this pipettor can satisfy the centre-to-centre spacing between the motion axis of adjacent injection chamber and be the basic centre-to-centre spacing of target plate household utensils, can not waste the rate of utilization in injection chamber again, not only can be applicable to conventional target plate household utensils, can also be applicable to subminiature target plate household utensils, has improved the preparation efficiency of target plate sample greatly, has higher economical and practical nature and scalability to solve above-mentioned defect and not enough that exist among the prior art at least partially.

SUMMERY OF THE UTILITY MODEL

In order to solve the technical problem, the utility model provides a pipettor. The pipette comprises a housing, a drive, 6 injection components and a transmission. The housing has an interior cavity. The driving device is arranged outside the shell. The injection parts are disposed through the housing, wherein the 6 injection parts are arranged in a 2 x 3 matrix form. At least a portion of the transmission is disposed in the internal cavity, the transmission being connected to each of the 6 injection members and the drive means to cause the 6 injection members to aspirate or expel fluid in synchrony.

Preferably, each of the injection components comprises a syringe, a plunger, a seal, and a pipetting head. The injection tube is disposed through the housing, an injection cavity is formed inside the injection tube, and a lower end of the injection tube has a convexo-concave portion. The plunger is disposed in the injection chamber and has an upper end connected to the actuator for movement between at least a maximum aspirating position and a maximum expelling position. The sealing member is disposed outside the plunger and at an upper portion of the injection chamber for sealing the injection chamber. The pipetting head is assembled at the convex-concave part through interference fit.

Preferably, the driving device comprises a motor, the motor is provided with a shaft extension, and the transmission device comprises a kinematic pair, a screw pair and a slide block. The kinematic pair is connected with the shell and comprises a driving synchronous pulley, a synchronous belt and a follow-up synchronous pulley, wherein the driving synchronous pulley is fixedly connected to the shaft extension. The screw pair is arranged in the inner cavity and comprises a screw fixedly connected with the follow-up synchronous belt wheel and a gap eliminating nut capable of moving up and down along the screw. The sliding block is arranged in the inner cavity, the sliding block is fixedly connected with the anti-backlash nut and can slide up and down along the screw rod, and the lower end of the sliding block is connected to the upper end of the plunger.

Preferably, the plunger includes a plunger body and a stepped portion provided at an upper end of the plunger body, and a lower end of the slider is provided with a notch, the notch includes a first inner diameter portion and a second inner diameter portion located at a lower end of the first inner diameter portion, an inner diameter of the first inner diameter portion is greater than or equal to an outer diameter of the stepped portion, an inner diameter of the second inner diameter portion is smaller than an inner diameter of the first inner diameter portion to form an abutting surface in the notch, and an inner diameter of the second inner diameter portion is equal to an outer diameter of the plunger body, and the stepped portion can be fitted with the abutting surface above the abutting surface.

Preferably, an adjusting jackscrew and a first elastic part are arranged in the first inner diameter part, the first elastic part abuts against the step part above the step part, the upper end of the adjusting jackscrew is connected with the sliding block, and the lower end of the adjusting jackscrew abuts against the upper end of the first elastic part.

Preferably, the transmission device further comprises a guide rail pair, and the guide rail pair comprises an optical axis and a linear bearing. The optical axis is fixedly arranged in the inner cavity. The linear bearing is arranged outside the optical axis and can slide up and down along the optical axis, and the linear bearing is further arranged in the sliding block. The sliding block can slide along the guide rail pair.

Preferably, the pipette further comprises a positioning mechanism comprising a positioning tab and a reference position sensor. The locating piece is arranged on the sliding block. The reference position sensor is provided on the housing.

Preferably, the pipettor further comprises a pipetting head withdrawing mechanism, wherein the pipetting head withdrawing mechanism comprises a push rod, a sliding pressing block and a second elastic piece. The ejector rod is vertically arranged in the inner cavity and can vertically slide relative to the shell. The sliding pressing block is arranged outside the injection tube and can slide along the injection tube, and the sliding pressing block is located below the ejector rod. The second elastic piece is arranged outside the ejector rod, the upper end of the second elastic piece is connected with the shell, and the lower end of the second elastic piece is connected with the sliding pressing block.

Preferably, the center-to-center distance between two adjacent injection parts is 9 mm.

According to the utility model discloses a pipettor, through setting up reasonable mode, the pipettor can dispose on the liquid-transfering workstation of different usage, not only can be applicable to conventional target plate household utensils, can also be applicable to super miniature target plate household utensils to under the prerequisite of the rate of utilization in extravagant injection chamber, the preparation efficiency of target plate sample has been improved greatly.

Drawings

Non-limiting and non-exhaustive embodiments of the present invention are described, by way of example, with reference to the following drawings, in which:

fig. 1 is a front view of a pipette according to an embodiment of the present invention, with a cover plate of a housing removed to clearly show the internal structure of the pipette;

fig. 2 is a cross-sectional view of the pipette shown in fig. 1 along line a-a in fig. 1;

fig. 3 is a schematic bottom view of the pipette shown in fig. 1;

fig. 4 is a process schematic diagram of a pipetting operation on a first target plate vessel using a pipette according to the present invention;

fig. 5 is a process schematic diagram of a pipetting operation on a second target plate vessel using a pipette according to the present invention; and

fig. 6 is a process schematic diagram of a pipetting operation on target plate vessel three using a pipette according to the present invention.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.

In the present invention, a pipette is provided. Fig. 1 is a front view of a pipette according to an embodiment of the present invention, with a cover plate of a housing removed to clearly show the internal structure of the pipette; fig. 2 is a cross-sectional view of the pipette shown in fig. 1 along line a-a in fig. 1; fig. 3 is a schematic bottom view of the pipette shown in fig. 1; fig. 4 is a process schematic diagram of a pipetting operation on a first target plate vessel using a pipette according to the present invention; fig. 5 is a process schematic diagram of a pipetting operation on a second target plate vessel using a pipette according to the present invention; and fig. 6 is a process schematic diagram of a pipetting operation on target plate vessel three using a pipette according to the present invention. A pipette 100 according to an embodiment of the present invention will be described in detail below with reference to fig. 1 to 6.

As shown in fig. 1-3, the illustrated pipette 100 includes a housing 110, a drive device 120 disposed outside of the

housing

110, 6 injection components 130 disposed through the housing 110, and a transmission 140. Wherein the injection component 130 and the transmission 140 constitute an actuator of the pipette 100.

As shown in fig. 1-3, the housing 110 has an inner cavity 115, and the inner cavity 115 may form a receiving space to receive at least a portion of a transmission 140 of the pipette 100 and an injection component 130 and the like. Specifically, in the present embodiment, the housing 110 includes an upper end block 2, a lower end block 13, and a frame 6 provided between the upper end block 2 and the lower end block 13. The upper end block 2 and the lower end block 13 may be connected to the frame 6 by welding or screw fastening, and together with the frame 6 enclose an inner cavity 115 having a front opening. Further, the housing 110 includes a cover plate 24 covering a front opening of the internal cavity 115, and the cover plate 24 may be detachably connected to the upper and

lower end blocks

2 and 3 by means of a threaded fastener connection so as to dispose at least a part of the transmission 140, the injection part 130, and the like of the pipette 100 in the internal cavity 115.

At least a portion of the housing 110 may be made of a relatively strong metallic material (e.g., aluminum, copper, steel, etc.) so as to provide the mechanical strength necessary to support the various components inside the housing 110. Preferably, in the present embodiment, the housing 110 is made of an aluminum alloy having a low density.

As shown in fig. 1, the driving device 120 is disposed outside the housing and is fixedly connected to the housing 110. Specifically, in the present embodiment, the driving device 120 is fixedly connected to the upper end block 2 of the housing 110. The drive device 120 is capable of energizing the injection component 130 during operation of the pipette 100.

As shown in fig. 1-3, the pipette 110 further includes 6 injection components 130, the injection components 130 being disposed through the housing 110. Specifically, in the present embodiment, the injection part 130 is vertically disposed through the lower end block 13 of the housing 110. The liquid can be sucked into the injection part 130 or discharged from the injection part 130. Further, 6 injection parts 130 are arranged in a matrix form of 2 × 3. Set up with this mode for through setting up reasonable mode, the utility model discloses a pipettor 100 can dispose on the liquid-transfering workstation of different usage, not only can be applicable to conventional target plate household utensils, can also be applicable to super miniature target plate household utensils to under the prerequisite of not extravagant injection chamber rate of utilization, improved the preparation efficiency of target plate sample greatly. The operation of pipetting with the pipette 100 of the present invention will be described in more detail later with reference to fig. 4 to 6.

As shown in fig. 1 to 2, a transmission 140 is connected to each of the 6 injection parts 130 and the driving device 120 to make the 6 injection parts 130 perform pipetting or discharging simultaneously. Specifically, in the present embodiment, at least a portion of the transmission device 140 is disposed in the inner cavity 115 of the housing 110 so as to be connected to each of the 6 injection components 130 in the inner cavity 115 of the housing 110, and the transmission device 140 is connected to the driving device 120, so that the driving device 120 can drive the 6 injection components 130 to perform liquid suction or liquid discharge synchronously through the transmission device 140, thereby ensuring the consistency of the actions of the 6 injection components 130.

As shown in fig. 1-2, each of the injection components 130 includes a syringe 14, a plunger 17, a seal 26, and a pipetting head 16. Syringe 14 is disposed through housing 110 and is fixedly disposed relative to housing 110. Injection tube 14 is internally formed with an injection cavity 131, and the lower end of injection tube 14 has a convexo-concave portion. Specifically, in the present embodiment, the injection tube 14 is disposed through the lower end block 13 of the housing 110. A plunger 17 is disposed in the injection chamber 131, the upper end of the plunger 17 being connected to the actuator 140 to be movable at least between a maximum aspirating position and a maximum expelling position. It should be noted that the "maximum pipetting position" referred to herein is a position at which the plunger 17 is positioned when the maximum pipetting volume of the injection member 130 (specifically, the pipetting head 16 of the maximum volume or the pipetting range of the injection member 130, which will be described in detail later) is aspirated, and it is understood that in the present embodiment, the maximum pipetting position is a position at which the lower end of the plunger 17 is positioned at the uppermost end of the injection chamber 131. Accordingly, the "maximum discharge position" refers to the position of the plunger 17 at which the liquid is completely discharged out of the injection part 130. When the plunger 17 moves toward the maximum suction position, the injection part 130 sucks liquid, and when the plunger 17 moves toward the maximum discharge position, the injection part 130 discharges liquid. A sealing member 26 is provided outside the plunger 17 and at an upper portion of the injection chamber 131 for sealing the injection chamber 131. Pipetting head 16 fits over the lower end of syringe 14 at a dimple with an interference fit to achieve a static seal. Wherein pipetting tips 16 having different ranges may fit in the indentations of the lower end of syringe 14, as desired. For example, pipetting head 16 may be a standardized pipetting head having a volume of 10 microliters to 1000 microliters. The pipetting head 16 is a disposable item, usually made of plastic, which is a consumable item and is discarded after a single liquid treatment. And wherein syringe 14 can be inserted with pipetting head 16 by moving axially downward with housing 100 to insert different ranges of disposable pipetting heads 16 with the male-female configuration of syringe 14.

During the pipetting process, the pipetting head 16 is immersed in the liquid to be pipetted, the plunger 17 is driven by the transmission device 140 to move upwards towards the maximum pipetting position, and the liquid is pipetted into the pipetting head 16; during the draining process, the plunger 17 is moved downwards by the actuator 140 towards the maximum draining position, and the liquid is drained out of the pipetting head 16. It should be noted that during pipetting or draining, the liquid does not enter the injection chamber 131, but merely stays in the pipetting head 16, thereby avoiding cross-contamination of the liquid. Thus, the size of the range of each liquid treatment depends on the volume of the pipetting head 16 used, while the size of the range of liquid treatments depends on the volume of the plunger 17 or the maximum pipetting position of the plunger 17, wherein the maximum pipetting position of the plunger 17 can be configured such that during pipetting the liquid just fills the maximum volume or range of the pipetting head 16 without entering the injection chamber 131.

As shown in fig. 1-2, injection component 130 further includes a seal adjustment nut 12 to seat seal 26 against an upper portion of injection chamber 131. Specifically, in the present embodiment, the sealing member 26 is an O-ring and is interposed between the syringe 14 and the seal adjusting nut 12, and the O-ring 26 and the seal adjusting nut 12 can connect the syringe 14 and the plunger 17 in a sliding and sealing manner, so as to realize a sliding seal. During pipetting, the actuator 140 moves the plunger 17 upward in the axial direction of the plunger 17 so that liquid is aspirated into the pipetting head 16, and during pipetting, the actuator 140 moves the plunger 17 downward in the axial direction of the plunger 17 so that liquid is pipetted out of the pipetting head 16.

As shown in fig. 1 to 2, the driving device 120 includes a motor 1, the motor 1 has a shaft extension 121, and the transmission device 140 includes a kinematic pair 141, a screw pair 142, and a slider 10.

As shown in fig. 1 to 2, the kinematic pair 141 is connected to the housing 110, and the kinematic pair 141 includes a driving timing pulley 3, a timing belt 4, and a follower timing pulley 5, wherein the driving timing pulley 3 is connected to the shaft extension 121 of the motor 1. Specifically, in the present embodiment, the kinematic pair 141 is connected with the upper end block 2 of the housing 110, the driving synchronous pulley 3 is fixedly connected to the shaft extension 121 of the motor 1 to be capable of moving together with the shaft extension 121 of the motor 1, and the internal teeth of the synchronous belt 4 can be meshed with the external teeth of the driving synchronous pulley 3 and the driven synchronous pulley 5, so that when the shaft extension 121 of the motor 1 drives the driving synchronous pulley 3 to rotate, the driving synchronous pulley 3 can drive the driven synchronous pulley 5 to rotate together with the synchronous belt 4. Preferably, the motor 1 is further provided with an encoder (not shown) to control the rotation speed, the forward and reverse rotation, and other motion parameters of the motor 1.

The screw pair 142 is arranged in the inner cavity 115, and the screw pair 142 comprises a screw 8 fixedly connected with the follow-up synchronous pulley 5 and an anti-backlash nut 20 capable of moving up and down along the screw 8. Wherein, the anti-backlash nut 20 is in threaded connection with the screw 8, so that the anti-backlash nut 20 can move up or down along the axial direction of the screw 8. Preferably, the screw pair 142 further comprises an upper bearing 23 and a lower bearing 25 disposed outside the screw 8, wherein the upper bearing 23 is further fixedly disposed in the upper end block 2 of the housing 110, and the lower bearing 25 is fixedly disposed in the lower end block 13 of the housing 110. In addition, the upper bearing 23, the lower bearing 25 and the screw 8 are coaxially arranged, so that the screw 8 is kept in a vertical state in the rotating process, and the anti-backlash nut 20 is driven to axially move upwards or downwards.

The sliding block 10 is arranged in the inner cavity 115, the sliding block 10 is fixedly connected with the anti-backlash nut 20 and can slide up and down along the screw 8, and the lower end of the sliding block 10 is connected to the upper end of the plunger 17. As the screw 8 can drive the anti-backlash nut 20 to move axially upwards or downwards, the anti-backlash nut 20 is fixedly connected with the slider 10, and the lower end of the slider 10 is connected to the upper end of the plunger 17, therefore, when the follow-up synchronous belt wheel 5 drives the screw 8 to rotate, the slider 10 can slide up and down along the screw 8 to drive the plunger 17 to move up and down, and then liquid suction or liquid discharge is performed.

As shown in fig. 1 to 2, the plunger 17 includes a plunger body and a step 171 provided at an upper end of the plunger body. The step 171 is connected to the plunger body and protrudes from the plunger body. The lower end of the slider 10 is provided with a notch 28. Notch 28 includes a first inner diameter portion and a second inner diameter portion at a lower end of the first inner diameter portion. The inner diameter of the first inner diameter portion is greater than or equal to the outer diameter of the step portion 171 of the plunger 17. The inner diameter of the second inner diameter portion is smaller than the inner diameter of the first inner diameter portion to form an abutting face in the notch 28, and the inner diameter of the second inner diameter portion is equal to the outer diameter of the plunger body, and the step portion 171 of the plunger 17 can be fitted with the abutting face above the abutting face. In this way, the plunger 17 is arranged so as not to be displaced in the horizontal direction with respect to the slider 10, which is advantageous for the precision of the injection member 130 during pipetting.

As shown in fig. 1-2, in this embodiment, the abutment faces in the notch 28A are perpendicular to the longitudinal axis of the plunger 17. The first elastic member 18 and the adjusting jack 19 are also provided in the first inner diameter portion of the notch 28 of the slider 10. Wherein the first elastic member 18 is disposed above the step 171 of the plunger 17 and abuts against the step 171 of the plunger 17. The adjustment jack 19 is disposed above the first elastic member 18. Wherein, the upper end of adjusting jackscrew 19 is connected with slider 10, and the lower extreme of adjusting jackscrew 19 supports against the upper end of first elastic component 18. In the operating state of pipette 100, first resilient member 18 is in a compressed state or a naturally extended state. Arranged in this way, the plunger 17 is not displaced in the vertical direction relative to the slide 10.

As shown in fig. 1-2, the transmission 140 further includes a guide rail pair 145, the guide rail pair 145 including the optical axis 7 and a linear bearing (not shown). The optical axis 7 is fixedly disposed in the inner cavity 115. Specifically, in the present embodiment, the guide rail pair 145 includes two optical axes 7, the two optical axes 7 are disposed side by side at intervals on both sides of the screw 8 and fixedly disposed between the upper end block 2 and the lower end block 13 through the slider 10, and a longitudinal axis of the optical axis 7 is parallel to a longitudinal axis of the screw 8. The linear bearing is provided outside the optical axis 7 and can slide up and down along the optical axis 7, and the linear bearing is also provided in the slider 10, wherein the slider 10 can slide along the guide rail pair 145. In this way, the optical axis 7 is designed as a sliding rail for the slider 10, which is used to guide the slider 10 to slide up and down along the optical axis 7, so that when the driving device 120 drives the screw 8 to rotate, the anti-backlash nut 20 drives the slider 10 to slide up and down along the optical axis 7, i.e. the slider 10 can slide along the guide rail pair 145.

As shown in fig. 1 to 2, the pipette 100 further includes a positioning mechanism 150, the positioning mechanism 150 includes a positioning piece 21 and a reference position sensor 22, the positioning piece 21 is disposed on the slider 10, and the reference position sensor 22 is disposed on the housing 110. Specifically, in the present embodiment, the positioning piece 21 is provided at the lower portion of the slider 10, and the reference position sensor 22 is provided at the upper surface of the lower end block 13 of the housing 110, the positioning piece 21 being vertically aligned with the reference position sensor 22, so that when the positioning piece 21 moves downward with the slider 10, the reference position sensor 22 can detect the position of the slider 10 by detecting the position of the positioning piece 21, and thus the initial position and the real-time position of the pipetting head 16 can be determined from the position of the slider 10. During initialization, the slide block 10 drives the plunger 17 to move downwards, and when the locating plate 21 on the slide block 10 detects an initial position signal, the operation is stopped, and the position is recorded as an initial position (reset). Preferably, the reference position sensor 22 may also be connected to an encoder of the motor 1 to feed back the real-time position of the slide 10 to the encoder. For example, the reference position sensor 22 may be an optoelectronic sensor.

Optionally, as shown in fig. 1 to 2, the pipette 100 further includes a pipette tip withdrawing mechanism 160, and the pipette tip withdrawing mechanism 160 includes a push rod 11, a sliding pressing block 15, and a second elastic member 27. The ram 11 is vertically disposed in the inner cavity 115 and is capable of sliding vertically relative to the housing 110. The sliding pressing block 15 is arranged outside the injection tube 14 and can slide along the injection tube 14, and the sliding pressing block 15 is positioned below the ejector rod 11. The second elastic member 27 is disposed outside the top bar 11, the upper end of the second elastic member 27 is connected to the housing 110, and the lower end of the second elastic member 27 is connected to the sliding pressing block 15. Specifically, in the present embodiment, the jack 11 is vertically disposed in the inner cavity 115 through the lower end block 13 of the housing 110, and the jack 11 is located below the slider 10 and fixedly connected to the sliding press block 15. In the pipetting mode, the second elastic member 27 is in a naturally stretched state. When the disposable pipetting head 16 needs to be unloaded after the completion of one pipetting process, the slider 10 can move downwards by a few millimeters beyond the stroke, the slider 10 moves downwards and pushes the ejector rod 11 and the sliding pressing block 15, and the sliding pressing block 15 applies pressure to the upper part of the pipetting head 16 arranged outside the injection tube 14, so that the pipetting head 16 falls off the injection tube 14. When the slider 10 no longer applies downward pressure to the plunger 11, the slide pressing piece 15 is restored by the elastic force of the second elastic member 27.

As shown in fig. 1 to 2, in the present embodiment, the slide block 15 of the pipetting head withdrawing mechanism 160 is directly sleeved outside the syringe 14, the plunger 11 and the slide block 15 are driven by the slider 10 to move downward, so that the pipetting head 16 can be withdrawn, and the driving device 120 of the plunger 17 is directly used as a driving device of the slide block 15, so that the structure is simple and compact, and the pipetting head withdrawing mechanism and the driving electromagnetic component with complicated structures are not required, thereby reducing the cost.

Preferably, as shown in fig. 3, the center-to-center distance between the adjacent two injection parts 130 is 9 mm. 6 injection part 130 arrange into 2 x 3's matrix form, make the utility model discloses a pipettor 100 can dispose under automatic framework on the liquid-transfering workstation of different usage, through chooseing for use reasonable mode, not only can be applicable to the target plate household utensils that basic centre-to-centre spacing is 9mm, can also be applicable to the various target plate household utensils that the centre-to-centre spacing is 4.5mm, 2.25mm to in the preparation process of sample, the extravagant passageway rate of utilization will not follow the mode of operation of pipettor 100 on the target plate of different specifications will be described in detail in combination with fig. 4 to 6.

Fig. 4 to 6 show target plate vessels that are currently standardized on the market.

Fig. 4 shows a target plate vessel 200, wherein the target plate vessel 200 comprises 96 receptacles 201 for receiving samples, the 96 receptacles 201 are arranged in an 8 × 12 matrix, and the center-to-center distance between two adjacent receptacles 201 is 9 mm. In preparing a sample using the target plate vessel 200, the pipette 100 first completes the sample preparation work on the 6 receiving portions 201 located at the site 0, and then moves the injection member 130 downward such that the injection member 130 is located above the 6 receiving portions 201 at the site 1 and completes the sample preparation work, and similarly, the injection member 130 is sequentially located above the

sites

2, 3, …, 15, respectively, and completes the sample preparation work, such that the sample prepared by the injection member 130 is uniformly distributed on each receiving portion 201 of the target plate vessel 200 and is distributed only in the receiving portion of the target plate vessel 200, and is not distributed outside the target plate vessel 200. Designed in this way, on the one hand, by using the pipette 100 of the present invention, samples can be prepared in 6 receiving portions 201 at the same time, which significantly improves the preparation efficiency of the samples, and on the other hand, the prepared samples can be uniformly distributed in the receiving portions 201 without falling on other areas outside the target plate vessel 200.

In fig. 5, a target plate vessel 300 is shown, wherein the target plate vessel 300 comprises 96 receptacles 301 for receiving samples, the 96 receptacles 301 are arranged in an 8 × 12 matrix, and the center-to-center distance between two adjacent receptacles 301 is 4.5 mm. In preparing a sample using the target plate vessel 300, the pipette 100 first completes the sample preparation work on the 6 receiving portions 301 located at the site 0, then moves the injection member 130 to above the 6 receiving portions 301 at the site 1 and completes the sample preparation work, and similarly, the injection member 130 is respectively located above the

sites

2, 3, …, 15 and completes the sample preparation work, so that the sample prepared by the injection member 130 is uniformly distributed on each receiving portion 301 of the target plate vessel 300 and is distributed only in the receiving portion of the target plate vessel 300, and is not distributed outside the target plate vessel 300. With this mode design, on the one hand, through using the utility model discloses a pipettor 100 can prepare the sample in 6 holding parts 301 simultaneously, obviously improves the preparation efficiency of sample, and on the other hand, through chooseing for use reasonable mode of operation, the sample of preparing can distribute evenly in holding part 301 that is in, and can not fall in other regions outside target plate household utensils 300.

In fig. 6, a target plate vessel 400 is shown, wherein the target plate vessel 400 comprises 96 receptacles 401 for receiving samples, the 96 receptacles 401 are arranged in an 8 × 12 matrix, and the center-to-center distance between two adjacent receptacles 401 is 2.25 mm. In preparing a sample using the target plate vessel 400, the pipette 100 first completes the sample preparation work on the 6 receiving portions 401 located at the site 0, then moves the injection member 130 to above the 6 receiving portions 401 at the site 1 and completes the sample preparation work, and similarly, the injection member 130 is respectively located above the

sites

2, 3, …, 15 and completes the sample preparation work, so that the sample prepared by the injection member 130 is uniformly distributed on each receiving portion 401 of the target plate vessel 400 and is distributed only in the receiving portion of the target plate vessel 400, and is not distributed outside the target plate vessel 400. With this mode design, on the one hand, through using the utility model discloses a pipettor 100 can prepare the sample in 6 holding portions 401 simultaneously, obviously improves the preparation efficiency of sample, and on the other hand, through chooseing for use reasonable mode of operation, the sample of preparing can distribute evenly in holding portion 401, and can not fall in other regions outside target plate household utensils 400.

Of course, the pipette 100 of the present disclosure is also suitable for any number of stacked target plate vessels, such as two, three, or more target plate vessels.

To sum up, the utility model discloses a pipettor 100 can dispose on the liquid-transfering workstation of different usage, not only can be applicable to conventional target plate household utensils, can also be applicable to super miniature target plate household utensils to under the prerequisite of the rate of utilization of not extravagant injection chamber, improved the preparation efficiency of target plate sample greatly, higher economical and practical nature and scalability have.

A pipetting method for preparing a sample using the pipette 100 according to a preferred embodiment of the present invention will be described in detail with reference to fig. 1 to 6.

When the liquid is moved to the beginning, motor 1 comes into operation in good time, and drive synchronous pulley 3 passes through hold-in range 4 drive follow-up synchronous pulley 5 and rotates to drive screw 8 and rotate, screw 8 drives slider 10 through eliminating the clearance nut 20 and slides from top to bottom along optical axis 7, and slider 10 can drive plunger 17 up-and-down motion in syringe 14, thereby carries out imbibition or flowing back. Wherein, when the pipetting head 16 is immersed in the liquid, the plunger 17 is moved upward and the liquid is sucked into the pipetting head 16, and when the plunger 17 is moved downward, the liquid is discharged from the pipetting head 16. It should be noted that during pipetting or draining, the liquid does not enter the injection chamber 131, but merely stays in the pipetting head 16, thereby avoiding cross-contamination of the liquid.

Any of the target plate vessels shown in fig. 4-6 was selected for sample preparation. For example, the target plate vessel 300 is selected to prepare a sample, as shown in fig. 5, the target plate vessel 300 includes 96 receptacles for receiving the sample, the 96 receptacles 301 are arranged in an 8 × 12 matrix, and the center-to-center distance between two adjacent receptacles 301 is 4.5 mm. In preparing a sample using the target plate vessel 300, the pipette 100 first completes the sample preparation work on the 6 receiving portions 301 located at the site 0, then moves the injection member 130 to above the 6 receiving portions 301 at the site 1 and completes the sample preparation work, and similarly, the injection member 130 is respectively located above the

sites

2, 3, …, 15 and completes the sample preparation work, so that the sample prepared by the injection member 130 is uniformly distributed on each receiving portion 301 of the target plate vessel 300 and is distributed only in the receiving portion of the target plate vessel 300, and is not distributed outside the target plate vessel 300.

The technical features of the above embodiments can be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the above embodiments are not described, but should be considered as the scope of the present specification as long as there is no contradiction between the combinations of the technical features.

While the invention has been described in connection with embodiments, it is to be understood by those skilled in the art that the foregoing description and drawings are merely illustrative and not restrictive of the broad invention, and that the invention is not limited to the disclosed embodiments. Various modifications and variations are possible without departing from the spirit of the invention.

Claims

1. A pipette, characterized in that it comprises:

a housing having an interior cavity;

a drive device disposed outside the housing;

6 injection parts disposed through the housing, wherein the 6 injection parts are arranged in a 2 x 3 matrix form; and

a transmission, at least a portion of which is disposed in the internal cavity, the transmission being connected to each of the 6 injection components and the drive means to cause the 6 injection components to aspirate or expel fluid in synchrony.

2. A pipette as defined in claim 1, wherein each of the injection components comprises:

a syringe disposed through the housing, having an injection cavity formed therein, and having a concave-convex portion at a lower end thereof;

a plunger disposed in the injection chamber, an upper end of the plunger being connected to the transmission for movement between at least a maximum aspirating position and a maximum expelling position;

a sealing member disposed outside the plunger and at an upper portion of the injection chamber for sealing the injection chamber; and

a pipetting head fitted at the relief by interference fit.

3. The pipette according to claim 2, wherein the drive device comprises a motor having a shaft extension, and the transmission device comprises:

the kinematic pair is connected with the shell and comprises a driving synchronous pulley, a synchronous belt and a follow-up synchronous pulley, wherein the driving synchronous pulley is fixedly connected to the shaft extension;

the screw pair is arranged in the inner cavity and comprises a screw fixedly connected with the follow-up synchronous belt wheel and a clearance elimination nut capable of moving up and down along the screw; and

the sliding block is arranged in the inner cavity, the sliding block is fixedly connected with the anti-backlash nut and can slide up and down along the screw rod, and the lower end of the sliding block is connected to the upper end of the plunger.

4. The pipette according to claim 3, wherein the plunger includes a plunger body and a stepped portion provided at an upper end of the plunger body, and a lower end of the slider is provided with a notch including a first inner diameter portion and a second inner diameter portion located at a lower end of the first inner diameter portion, an inner diameter of the first inner diameter portion being greater than or equal to an outer diameter of the stepped portion, an inner diameter of the second inner diameter portion being smaller than the inner diameter of the first inner diameter portion to form an abutting face in the notch, and an inner diameter of the second inner diameter portion being equal to an outer diameter of the plunger body, the stepped portion being capable of fitting with the abutting face above the abutting face.

5. The pipette according to claim 4, wherein an adjustment tip and a first elastic member are provided in the first inner diameter portion, the first elastic member abuts against the step portion above the step portion, an upper end of the adjustment tip is connected to the slider, and a lower end of the adjustment tip abuts against an upper end of the first elastic member.

6. The pipette of claim 3, wherein the transmission further comprises a rail pair, the rail pair comprising:

an optical axis fixedly disposed within the inner chamber; and

a linear bearing disposed outside the optical axis and capable of sliding up and down along the optical axis, the linear bearing further disposed in the slider,

wherein the sliding block can slide along the guide rail pair.

7. A pipette as defined in claim 3, further comprising a positioning mechanism comprising:

the positioning piece is arranged on the sliding block; and

a reference position sensor disposed on the housing.

8. The pipette of claim 2, further comprising a pipetting head exit mechanism comprising:

the ejector rod is vertically arranged in the inner cavity and can vertically slide relative to the shell;

the sliding pressing block is arranged outside the injection tube and can slide along the injection tube, and the sliding pressing block is positioned below the ejector rod; and

the second elastic piece is arranged outside the ejector rod, the upper end of the second elastic piece is connected with the shell, and the lower end of the second elastic piece is connected with the sliding pressing block.

9. The pipette according to claim 1, wherein a center-to-center distance between adjacent two of the injection members is 9 mm.