CN106420516A - Red onion extract containing skin-care product and application thereof - Google Patents
Red onion extract containing skin-care product and application thereof Download PDFInfo
- Publication number
- CN106420516A CN106420516A CN201610513885.4A CN201610513885A CN106420516A CN 106420516 A CN106420516 A CN 106420516A CN 201610513885 A CN201610513885 A CN 201610513885A CN 106420516 A CN106420516 A CN 106420516A
- Authority
- CN
- China
- Prior art keywords
- extract
- care product
- skin care
- ethyl acetate
- shallot
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
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- 229940072113 onion extract Drugs 0.000 title abstract description 5
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- 230000001815 facial effect Effects 0.000 claims abstract description 18
- 230000003020 moisturizing effect Effects 0.000 claims abstract description 6
- 239000000463 material Substances 0.000 claims abstract description 5
- 244000291564 Allium cepa Species 0.000 claims description 57
- 235000010167 Allium cepa var aggregatum Nutrition 0.000 claims description 56
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 claims description 26
- 239000000047 product Substances 0.000 claims description 24
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- 238000002360 preparation method Methods 0.000 claims description 21
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/005—Antimicrobial preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Dermatology (AREA)
- Microbiology (AREA)
- Epidemiology (AREA)
- Birds (AREA)
- Mycology (AREA)
- Botany (AREA)
- Biotechnology (AREA)
- Gerontology & Geriatric Medicine (AREA)
- Engineering & Computer Science (AREA)
- Cosmetics (AREA)
Abstract
Description
技术领域technical field
本发明涉及日用化妆品技术领域,具体地,涉及一种含红葱提取物的护肤品及其用途。The invention relates to the technical field of daily cosmetics, in particular to a skin care product containing shallot extract and its application.
背景技术Background technique
红葱属于草本植物,为鸢尾科(Iridaceae)红葱属(EleutherineamericanaMerr.et K.Heyne)植物。鸢尾科红葱是西南地区尤其云南省傣族、彝族、德昂族和当地汉族等各民族常用的草药,民间别名众多,如小红蒜、百步还阳、万娘(德昂族名)、楼子葱、怕波亮(傣语)等。红葱原产于西印度群岛、美洲等地,我国最早在云南民间引入栽培种。其鳞茎卵圆形,直径约2.5厘米,鳞片肥厚,紫红色,无膜质包被。叶宽披针形或宽条形,4-5条纵脉平行而突出,使叶表面呈现明显的皱褶。Shallot belongs to herbaceous plants, and is a plant of the genus EleutherineamericanaMerr.et K.Heyne in the family Iridaceae. Red onion of the family Iridaceae is a herbal medicine commonly used by the Dai, Yi, De'ang and local Han in Southwest China, especially in Yunnan Province. Green onions, afraid of wave bright (Dai language) and so on. Red onion is native to the West Indies, the Americas and other places. my country first introduced cultivated species in Yunnan. Its bulb is oval, about 2.5 cm in diameter, with thick, purple-red scales and no membranous coating. The leaves are broadly lanceolate or strip-shaped, with 4-5 parallel and prominent longitudinal veins, making the surface of the leaves show obvious wrinkles.
红葱有清热凉血、活血散瘀、利尿除湿、消肿解毒之功效,临床主要用于治疗冠状动脉粥样硬化心脏病,月经不调、痛经、闭经及崩漏等妇科疾病以及胃癌。目前已在红葱中分离出蒽醌、萘酚、萘醌及其衍生物等主要化学成分,具有抗菌、抗氧化、抗肿瘤和抗HIV病毒等作用。已有文献报道了红葱乙醇提取物对人类和禽类的弯曲杆菌有明显抑菌效应,红葱甲醇提取物对金黄色葡萄球菌以及香蕉炭疽病菌、百合炭疽病菌、番茄灰霉病菌等几种植物病原菌有较好的抑制作用,红葱中三个主要药理活性成分红葱甲素、红葱乙素和异红葱甲素,也有显著的抗菌作用。红葱粗提物对DPPH•和•OH有清除作用,有人将其作为一种天然食品添加剂应用于猪肉中,是一种潜在的新型天然抑制脂质过氧化的食品抗氧化剂。红葱乙醇提取物能显著增强血液中SOD 活性及GSH-Px活性,还能有效抑制酪氨酸酶的活性。红葱乙素能抑制黑色素形成,日本企业已将红葱甲素作为美白因子应用于功能性美白护肤品当中,可见红葱提取物含有皮肤美白活性功能成分,是一种很有市场前景的植物活性成分原料。Red onion has the effects of clearing heat and cooling blood, promoting blood circulation and dispelling blood stasis, diuresis and dehumidification, reducing swelling and detoxification. It is mainly used in the treatment of coronary atherosclerotic heart disease, irregular menstruation, dysmenorrhea, amenorrhea, metrorrhagia and other gynecological diseases, as well as gastric cancer. At present, the main chemical components such as anthraquinone, naphthol, naphthoquinone and their derivatives have been isolated from shallots, which have antibacterial, antioxidative, antitumor and anti-HIV effects. It has been reported in the literature that the ethanol extract of red onion has obvious antibacterial effect on Campylobacter in humans and poultry. Pathogenic bacteria have a good inhibitory effect, and the three main pharmacologically active ingredients in shallots, shallot A, shallot B and isoretin A, also have significant antibacterial effects. The crude extract of red onion has a scavenging effect on DPPH• and •OH. Some people use it as a natural food additive in pork, and it is a potential new natural food antioxidant that inhibits lipid peroxidation. Red onion ethanol extract can significantly enhance the activity of SOD and GSH-Px in the blood, and can also effectively inhibit the activity of tyrosinase. Shallot B can inhibit the formation of melanin. Japanese companies have used shallot A as a whitening factor in functional whitening skin care products. It can be seen that shallot extract contains skin whitening active functional ingredients, and is a plant with great market prospects Active ingredient raw material.
随着人们生活水平的提高,“天然”“有机”的食品和化妆品越来越得到青睐,红葱具有药食同源性,以红葱为新资源在食品加工、功能食品、植物饲料添加剂、功能性化妆品、植物原料药等方面有很好的应用前景。但是其开发利用有很大的地域局限性,因此,以安全性为前提,开发天然植物为主要功效成分,以改善人们的面部肌肤,延缓衰老,促进皮肤微循环,抗菌消炎为目的是十分有意义的。目前,尚无红葱乙酸乙酯提取部位应用于化妆品的报道。发明内容With the improvement of people's living standards, "natural" and "organic" food and cosmetics are becoming more and more popular. Red onion has the same origin as medicine and food. Using red onion as a new resource is widely used in food processing, functional food, plant feed additives, It has good application prospects in functional cosmetics and plant raw materials. However, its development and utilization have great regional limitations. Therefore, on the premise of safety, it is very effective to develop natural plants as the main functional components to improve people's facial skin, delay aging, promote skin microcirculation, and antibacterial and anti-inflammatory. meaningful. At present, there is no report on the application of ethyl acetate extraction parts of shallots in cosmetics. Contents of the invention
本发明针对现有技术的不足,提供一种含红葱提取物的护肤品,该护肤品能有效改善面部肌肤,清除自由基,促进皮肤微循环,具有抗菌消炎,延缓衰老,美白保湿及祛痘的效果。The present invention aims at the deficiencies of the prior art, and provides a skin care product containing shallot extract, which can effectively improve facial skin, remove free radicals, promote skin microcirculation, have antibacterial and anti-inflammatory properties, delay aging, whiten, moisturize and remove acne effect.
为实现上述目的,本发明是通过以下方案给予实现的:To achieve the above object, the present invention is achieved by the following schemes:
一种含红葱提取物的护肤品,含有如下重量百分数的各组分:红葱乙酸乙酯提取物3~15%,仙人掌提取物1~5%,积雪草提取物1~3%,辅料78~95%。A skin care product containing red onion extract, which contains the following components by weight percentage: 3-15% of ethyl acetate extract of red onion, 1-5% of cactus extract, 1-3% of centella asiatica extract, Excipients 78-95%.
红葱中三个主要药理活性成分为红葱甲素、红葱乙素和异红葱甲素,有显著的抗菌作用。红葱乙素能抑制黑色素形成,红葱甲素作为一种美白因子,能够改善皮肤功能。红葱还含有多种萘醌、萘酚、蒽醌、葡萄糖苷化合物,能促进肌肤血液循环,抑制酪氨酸酶的活性,淡化黑色素;能够迅速渗入炎症部位,消炎症,止胀痛;能够清除自由基,增强皮肤弹性肌的活性;仙人掌提取物具有抗氧化及延缓衰老的作用;积雪草提取物可以紧致表皮与真皮连接部分,能使皮肤变柔软,改善皮肤松弛,使皮肤光滑有弹性,促进真皮层中胶原蛋白形成,使纤维蛋白再生,使肌肤达到紧致光滑的效果。还可帮助受损的组织愈合及紧实肌肤。The three main pharmacologically active ingredients in shallots are shallot A, shallot B and iso-shale, which have significant antibacterial effects. Shallot B can inhibit the formation of melanin, and Shallot A, as a whitening factor, can improve skin function. Red onion also contains a variety of naphthoquinones, naphthols, anthraquinones, and glucoside compounds, which can promote blood circulation in the skin, inhibit the activity of tyrosinase, and dilute melanin; it can quickly penetrate into the inflammation site, reduce inflammation, and relieve pain; Scavenge free radicals and enhance the activity of skin elastic muscles; cactus extract has anti-oxidation and anti-aging effects; centella asiatica extract can tighten the connection between the epidermis and dermis, make the skin soft, improve skin relaxation, and make the skin smooth It is elastic, promotes the formation of collagen in the dermis, regenerates fibrin, and makes the skin firm and smooth. It also helps damaged tissue heal and firm the skin.
优选地,所述护肤品的剂型为面霜、乳液、面膜、精华液、洁面乳、爽肤水、柔肤水、紧肤水、营养化妆水、粉饼、体露或身体乳。Preferably, the dosage form of the skin care product is face cream, lotion, mask, essence, cleanser, toner, toner, toner, nutrient lotion, powder cake, body lotion or body milk.
以红葱乙酸乙酯提取物制备的护肤品优选为面霜,面霜含有以下重量百分数的各组分:红葱乙酸乙酯提取物3~15%,仙人掌提取物1~5%,积雪草提取物1~3%,基础油5~15%,保湿剂3~10%,乳化剂3~10%,促透剂0.5~1%,赋形剂0.1~1%,抗氧化剂0.1~1%,防腐剂0.1~0.6%,香精0.01~0.05%,水余量。The skin care product prepared from shallot ethyl acetate extract is preferably face cream, and the face cream contains the following components in weight percentage: 3-15% of shallot ethyl acetate extract, 1-5% of cactus extract, Centella asiatica extract 1-3% of base oil, 5-15% of base oil, 3-10% of humectant, 3-10% of emulsifier, 0.5-1% of penetration enhancer, 0.1-1% of excipient, 0.1-1% of antioxidant, Preservative 0.1-0.6%, essence 0.01-0.05%, water balance.
作为优选实施方式,面霜中还可以加入0.1~0.5%的防晒剂。所述防晒剂为4-甲基亚苄基樟脑。As a preferred embodiment, 0.1-0.5% sunscreen agent can also be added to the face cream. The sunscreen is 4-methylbenzylidene camphor.
以红葱乙酸乙酯提取物制备的护肤品优选为乳液,乳液含有以下重量百分数的各组分:红葱乙酸乙酯提取物3~15%,仙人掌提取物1~5%,积雪草提取物1~3%,基础油3~10%,保湿剂3~10%,乳化剂5~10%,抗氧化剂0.1~0.5%,角质软化剂0.1~0.3%,防腐剂0.1~0.6%,免疫调节剂1~3%,香精0.01~0.05%,水余量。The skin care product prepared from shallot ethyl acetate extract is preferably an emulsion, and the emulsion contains the following components in weight percentage: 3-15% of shallot ethyl acetate extract, 1-5% of cactus extract, Centella asiatica extract 1% to 3% base oil, 3% to 10% base oil, 3% to 10% moisturizer, 5% to 10% emulsifier, 0.1% to 0.5% antioxidant, 0.1% to 0.3% cuticle softener, 0.1% to 0.6% preservative, immune Regulator 1-3%, essence 0.01-0.05%, water balance.
更优选地,所述乳液含有以下重量百分数的各组分:红葱乙酸乙酯提取物3~15%,仙人掌提取物1~5%,积雪草提取物1~3%,基础油3~5%,保湿剂5~8%,乳化剂5~8%,抗氧化剂0.1~0.5%,角质软化剂0.1%,防腐剂0.5~0.6%,免疫调节剂1~2%,香精0.01~0.05%,水余量。More preferably, the emulsion contains the following components in weight percentage: 3-15% of ethyl acetate extract of red onion, 1-5% of cactus extract, 1-3% of centella asiatica extract, 3-3% of base oil 5%, moisturizer 5-8%, emulsifier 5-8%, antioxidant 0.1-0.5%, cuticle softener 0.1%, preservative 0.5-0.6%, immunomodulator 1-2%, essence 0.01-0.05% , the water balance.
作为优选实施方式,乳液中还可以加入0.1~1%的促透剂。As a preferred embodiment, 0.1-1% of a penetration enhancer can also be added to the emulsion.
更优选地,乳液中的免疫调节剂为羧甲基葡聚糖。More preferably, the immunomodulator in the emulsion is carboxymethyl dextran.
以红葱乙酸乙酯提取物制备的护肤品优选为面膜,面膜含有以下重量百分数的各组分:红葱乙酸乙酯提取物3~15%,仙人掌提取物1~5%,积雪草提取物1~3%,保湿剂3~15%,乳化剂1~5%,角质软化剂0.1~0.3%,抗氧化剂0.1~0.5%,防腐剂0.1~0.6%,香精0.01~0.05%,水余量。The skin care product prepared with red onion ethyl acetate extract is preferably a mask, and the mask contains the following components in weight percentages: red onion ethyl acetate extract 3-15%, cactus extract 1-5%, centella asiatica extract 1% to 3% moisturizers, 3% to 15% moisturizers, 1% to 5% emulsifiers, 0.1% to 0.3% cutin softeners, 0.1% to 0.5% antioxidants, 0.1% to 0.6% preservatives, 0.01% to 0.05% essences, and water quantity.
更优选地,所述面膜,含有以下重量百分数的各组分:红葱乙酸乙酯提取物3~15%,仙人掌提取物1~5%,积雪草提取物1~3%,保湿剂5~10%,乳化剂1~2%,角质软化剂0.1~0.2%,抗氧化剂0.3~0.5%,防腐剂0.1~0.3%,香精0.01~0.05%,水余量。More preferably, the facial mask contains the following components in weight percentage: 3-15% ethyl acetate extract of red onion, 1-5% cactus extract, 1-3% centella asiatica extract, 5% moisturizing agent ~10%, emulsifier 1~2%, cuticle softener 0.1~0.2%, antioxidant 0.3~0.5%, preservative 0.1~0.3%, essence 0.01~0.05%, water balance.
更优选地,所述面膜中还可以加入0.1~0.2%的促透剂。More preferably, 0.1-0.2% of a penetration enhancer can also be added to the facial mask.
更优选地,所述面膜中还可以加入0.2~0.5%的赋形剂。More preferably, 0.2-0.5% of excipients can also be added to the facial mask.
以红葱乙酸乙酯提取物制备的护肤品优选为精华液,精华液含有以下重量百分数的各组分:红葱乙酸乙酯提取物3~15%,仙人掌提取物1~5%,积雪草提取物1~3%,保湿剂3~15%,乳化剂1~5%,防腐剂0.1~0.6%,香精0.01~0.05%,水余量。The skin care product prepared from shallot ethyl acetate extract is preferably an essence, and the essence contains the following components in weight percentage: 3-15% of shallot ethyl acetate extract, 1-5% of cactus extract, snow Grass extract 1-3%, humectant 3-15%, emulsifier 1-5%, preservative 0.1-0.6%, essence 0.01-0.05%, water balance.
更优选地,所述精华液含有以下重量百分数的各组分:红葱乙酸乙酯提取物3~15%,仙人掌提取物1~5%,积雪草提取物1~3%,保湿剂5~13%,乳化剂1~2%,抗氧化剂0.1~1%,防腐剂0.5%,香精0.01~0.05%,水余量。More preferably, the essence contains the following components in weight percentage: 3-15% ethyl acetate extract of red onion, 1-5% cactus extract, 1-3% centella asiatica extract, 5% moisturizing agent ~13%, emulsifier 1~2%, antioxidant 0.1~1%, preservative 0.5%, essence 0.01~0.05%, water balance.
更优选地,所述精华液中还可以加入0.1~1%的促透剂;1~5%的基础油;0.1~0.3%的角质软化剂;0.01~0.05%的络合剂。所述络合剂为EDTA二钠。More preferably, 0.1-1% of penetration enhancer; 1-5% of base oil; 0.1-0.3% of cuticle softener; 0.01-0.05% of complexing agent can also be added to the essence. The complexing agent is disodium EDTA.
最优选地,以上面霜、乳液、面膜、精华液中所述的乳化剂选自氢化卵磷脂、鲸蜡醇和鲸蜡硬脂基葡糖苷、辛酸/葵酸甘油三酯、甘油硬脂酸酯、鲸蜡硬脂醇醚-21、山梨坦硬脂酸酯、月桂醇聚醚、单硬脂酸甘油酯或聚乙烯醇中的一种或多种。Most preferably, the emulsifiers described above in creams, lotions, facial masks, and serums are selected from hydrogenated lecithin, cetyl alcohol and cetearyl glucoside, caprylic/capric triglyceride, glyceryl stearate, One or more of cetearyl ether-21, sorbitan stearate, lauryl ether, glyceryl monostearate or polyvinyl alcohol.
最优选地,以上面霜、乳液、面膜、精华液中所述的保湿剂选自植物甾醇酯、1,3-丁二醇、聚乙二醇-6、甘油、透明质酸、神经酰胺、三乙醇胺、十四酸异丙酯、海藻糖、三甲基甘氨酸、丙二醇、十六醇、双丙甘醇、蔗糖、异十六烷、L-吡咯烷酮羧酸钠或甘草酸二钾中的一种或多种。Most preferably, the moisturizing agent described in the above face cream, lotion, facial mask, and essence is selected from phytosterol esters, 1,3-butanediol, polyethylene glycol-6, glycerin, hyaluronic acid, ceramide, three One of ethanolamine, isopropyl myristate, trehalose, trimethylglycine, propylene glycol, cetyl alcohol, dipropylene glycol, sucrose, isohexadecane, sodium L-pyrrolidone carboxylate, or dipotassium glycyrrhizinate or more.
最优选地,以上面霜、乳液、面膜、精华液中所述的基础油选自乳木果油、二甲基硅氧烷、环甲基硅氧烷、聚二甲基硅氧烷、环五聚二甲基硅氧烷、角鲨烷或杏仁油中的一种或多种。Most preferably, the base oil described in the above face cream, lotion, mask, and essence is selected from shea butter, dimethylsiloxane, cyclomethicone, polydimethylsiloxane, cyclopenta One or more of dimethicone, squalane, or almond oil.
最优选地,以上面霜、乳液、面膜、精华液中所述的抗氧化剂为丁羟甲苯或生育酚乙酸酯。Most preferably, the antioxidant described in the cream, lotion, facial mask, and essence is butylated hydroxytoluene or tocopheryl acetate.
最优选地,以上面霜、乳液、面膜、精华液中所述的防腐剂选自羟苯甲酯、羟苯丙酯或苯氧乙醇中的一种或多种。Most preferably, the preservatives described in the above face cream, lotion, facial mask and essence are selected from one or more of methylparaben, propylparaben or phenoxyethanol.
最优选地,以上面霜、乳液、面膜、精华液中所述的促透剂选自PEG-40氢化蓖麻油。Most preferably, the penetration enhancer described in the above face cream, lotion, facial mask, and essence is selected from PEG-40 hydrogenated castor oil.
最优选地,以上乳液、面膜、精华液中所述的角质软化剂为尿囊素。Most preferably, the cuticle softening agent described in the above lotion, facial mask, and essence is allantoin.
更优选地,以上所述赋形剂为汉生胶或卡波姆的一种或多种。More preferably, the above excipients are one or more of xanthan gum or carbomer.
更优选地,如上所述红葱乙酸乙酯提取物的制备方法为:More preferably, the preparation method of the shallot ethyl acetate extract as mentioned above is:
S1.取干燥红葱块状物,加入60%乙醇,提取两次,每次提取1h,两次提取液合并、过滤;S1. Take the dried shallot block, add 60% ethanol, extract twice, each time for 1 hour, combine the two extracts, and filter;
S2.往S1过滤后的滤液中加入活性炭脱色,脱色温度85℃,过滤至溶液澄清无活性炭;S2. Add activated carbon to the filtrate filtered by S1 for decolorization, the decolorization temperature is 85°C, and filter until the solution is clear and free of activated carbon;
S3.将S2中滤液用乙酸乙酯萃取,乙酸乙酯部位液旋转蒸发回收溶剂;S3. The filtrate in S2 is extracted with ethyl acetate, and the ethyl acetate part liquid is rotary evaporated to recover the solvent;
S4.将S3中回收的溶剂浓缩干燥,即得红葱乙酸乙酯提取物。S4. Concentrate and dry the solvent recovered in S3 to obtain the ethyl acetate extract of shallot.
与现有技术相比,本发明具有以下有益效果:Compared with the prior art, the present invention has the following beneficial effects:
本发明提供的护肤效果显著,能有效改善面部肌肤,抗菌消炎,清除自由基,延缓衰老,促进皮肤微循环,兼具祛痘美白之功效,保湿效果好,对皮肤安全无刺激。The skin care effect provided by the invention is remarkable, can effectively improve facial skin, antibacterial and anti-inflammatory, scavenge free radicals, delay aging, promote skin microcirculation, has the effect of removing acne and whitening, has good moisturizing effect, and is safe and non-irritating to the skin.
附图说明Description of drawings
图1为红葱乙酸乙酯提取部位物对金黄色葡萄球菌ATCC25923抑菌活性。Figure 1 shows the antibacterial activity of shallot ethyl acetate extract fractions on Staphylococcus aureus ATCC25923.
图2为红葱乙酸乙酯提取部位物对大肠杆菌ATCC25922抑菌活性。Fig. 2 is the bacteriostatic activity of ethyl acetate extract fraction of shallot on Escherichia coli ATCC25922.
图3为红葱乙酸乙酯提取部位物对鼠伤寒沙门氏菌ATCC 14028抑菌活性。Fig. 3 is the bacteriostatic activity of shallot ethyl acetate extract fractions on Salmonella typhimurium ATCC 14028.
图4为红葱乙酸乙酯提取部位物对表皮金黄色葡萄球菌ATCC11827抑菌活性。Fig. 4 is the antibacterial activity of the extraction parts of shallot ethyl acetate on Staphylococcus aureus ATCC11827.
图5为红葱乙酸乙酯提取部位物对痤疮丙酸杆菌ATCC6919抑菌活性。Figure 5 shows the antibacterial activity of the ethyl acetate extract fraction of red onion against Propionibacterium acnes ATCC6919.
图6为红葱乙酸乙酯提取部位物对DPPH•的清除能力。Figure 6 shows the scavenging ability of shallot ethyl acetate extract fraction on DPPH•.
图7为红葱乙酸乙酯提取部位物对•OH的清除能力。Figure 7 shows the scavenging ability of shallot ethyl acetate extract fractions on •OH.
具体实施方式detailed description
下面结合说明书附图和具体实施例对本发明作出进一步地详细阐述,所述实施例只用于解释本发明,并非用于限定本发明的范围。下述实施例中所使用的试验方法如无特殊说明,均为常规方法;所使用的材料、试剂等,如无特殊说明,为可从商业途径得到的试剂和材料。The present invention will be further elaborated below in combination with the accompanying drawings and specific embodiments. The embodiments are only used to explain the present invention, and are not intended to limit the scope of the present invention. The test methods used in the following examples are conventional methods unless otherwise specified; the materials and reagents used are commercially available reagents and materials unless otherwise specified.
实施例1 红葱乙酸乙酯提取部位的制备Example 1 The preparation of the ethyl acetate extraction part of red onion
取干燥红葱块状物500g,加60%乙醇4L,提取两次,每次1h,提取液过滤,合并滤液,加入100g活性炭脱色,脱色温度85℃,过滤至溶液澄清无活性炭,滤液用等体积乙酸乙酯萃取,乙酸乙酯部位萃取液用旋转蒸发仪55~65℃回收溶剂,浓缩干燥即得红葱乙酸乙酯提取部位。Take 500g of dry shallot lumps, add 4L of 60% ethanol, extract twice, each time for 1h, filter the extract, combine the filtrate, add 100g of activated carbon for decolorization, the decolorization temperature is 85°C, filter until the solution is clear without activated carbon, the filtrate is used, etc. Volumetric ethyl acetate extraction, the ethyl acetate fraction extract was recovered with a rotary evaporator at 55-65°C, concentrated and dried to obtain the ethyl acetate extraction fraction of shallot.
实施例2 红葱提取物护肤霜1的制备Example 2 Preparation of Shallot Extract Skin Cream 1
A相:氢化卵磷脂35g,乳木果油20g,植物甾醇酯8g,二甲基硅氧烷30g,环甲基硅氧烷20g,鲸蜡醇和鲸蜡硬脂基葡糖苷10g,辛酸/葵酸甘油三酯40g,丁羟甲苯2g,PEG-40氢化蓖麻油5g,羟苯丙酯0.1g。Phase A: Hydrogenated Lecithin 35g, Shea Butter 20g, Phytosterol Esters 8g, Dimethicone 30g, Cyclomethicone 20g, Cetyl Alcohol and Cetearyl Glucoside 10g, Caprylic/Sunflower Triglyceride 40g, BHT 2g, PEG-40 hydrogenated castor oil 5g, propylparaben 0.1g.
B相:卡波姆4g,1,3-丁二醇20g,聚乙二醇-6 2g,汉生胶1g,甘油30g,透明质酸0.5g,羟苯甲酯1g,余量为去离子水(以1000g计)。Phase B: carbomer 4g, 1,3-butanediol 20g, polyethylene glycol-6 2g, xanthan gum 1g, glycerin 30g, hyaluronic acid 0.5g, methylparaben 1g, the balance is deionized Water (based on 1000g).
C相:神经酰胺0.2g,三乙醇胺2g,红葱乙酸乙酯提取物35g,仙人掌提取物10g,积雪草提取物20g。Phase C: 0.2g ceramide, 2g triethanolamine, 35g ethyl acetate extract of red onion, 10g cactus extract, 20g centella asiatica extract.
D相:苯氧乙醇4g,香精0.3g。Phase D: 4g of phenoxyethanol, 0.3g of essence.
制备:将A相加热到85℃,使其完全溶解,将B相加热至85℃,搅拌分散均匀,将A,B相混合均质并搅拌,趁热加三乙醇胺,当温度降到40℃以下时,加入C相、D相等并搅拌20分钟即得。Preparation: Heat phase A to 85°C to dissolve completely, heat phase B to 85°C, stir and disperse evenly, mix phases A and B homogeneously and stir, add triethanolamine while hot, when the temperature drops to 40°C At the following time, add phase C and phase D and stir for 20 minutes.
实施例3 红葱提取物护肤霜2的制备Example 3 Preparation of Shallot Extract Skin Cream 2
A相:辛酸/葵酸甘油三酯20g,棕榈酸乙基己酯20g,十四酸异丙酯20g,甘油硬脂酸酯10g,乳木果油20g,鲸蜡硬脂醇醚-21 10g,环甲基硅氧烷20g,鲸蜡醇和鲸蜡硬脂基葡糖苷10g,植物甾醇酯8g,4-甲基亚苄基樟脑3g,丁羟甲苯5g,羟苯丙酯0.2g,PEG-40氢化蓖麻油10 g。Phase A: Caprylic/Capric Triglyceride 20g, Ethylhexyl Palmitate 20g, Isopropyl Myristate 20g, Glyceryl Stearate 10g, Shea Butter 20g, Ceteareth-21 10g , cyclomethicone 20g, cetyl alcohol and cetearyl glucoside 10g, phytosterol ester 8g, 4-methylbenzylidene camphor 3g, butylated hydroxytoluene 5g, propylparaben 0.2g, PEG- 40 hydrogenated castor oil 10 g.
B相:甘油30g,海藻糖10g,1,3-丁二醇20g,汉生胶1g,透明质酸0.5g,羟苯甲酯1g,余量为去离子水(以1000g计)。Phase B: 30g of glycerin, 10g of trehalose, 20g of 1,3-butanediol, 1g of xanthan gum, 0.5g of hyaluronic acid, 1g of methylparaben, and the balance is deionized water (based on 1000g).
C相:神经酰胺0.2g,红葱乙酸乙酯提取物50g,仙人掌提取物20g,积雪草提取物15g。Phase C: ceramide 0.2g, shallot ethyl acetate extract 50g, cactus extract 20g, centella asiatica extract 15g.
D相:苯氧乙醇4g,香精0.5g。Phase D: 4g of phenoxyethanol, 0.5g of essence.
制备:将A相加热到85℃,使其完全溶解,将B相加热至85℃,搅拌分散均匀,将A,B相混合均质并搅拌,当温度降到40℃以下时,加入C相、D相等并搅拌20分钟即得。Preparation: Heat phase A to 85°C to dissolve completely, heat phase B to 85°C, stir and disperse evenly, mix phases A and B homogeneously and stir, when the temperature drops below 40°C, add phase C , D are equal and stirred for 20 minutes.
实施例4 红葱提取物护肤霜3的制备Example 4 Preparation of Shallot Extract Skin Cream 3
A相:乳木果油25g,辛酸/葵酸甘油三酯30g,山梨坦硬脂酸酯20g,植物甾醇酯10g,二甲基硅氧烷30g,环甲基硅氧烷10g,鲸蜡醇和鲸蜡硬脂基葡糖苷20g,棕榈酸异丙酯20g,丁羟甲苯2g,羟苯丙酯0.1g。Phase A: Shea Butter 25g, Caprylic/Capric Triglyceride 30g, Sorbitan Stearate 20g, Phytosterol Esters 10g, Dimethicone 30g, Cyclomethicone 10g, Cetyl Alcohol and Cetearyl glucoside 20g, isopropyl palmitate 20g, butylated hydroxytoluene 2g, propylparaben 0.1g.
B相:甘油60g,1,3-丁二醇20g,聚乙二醇-63g,汉生胶1.5g,透明质酸0.5g,羟苯甲酯2g,余量为去离子水(以1000g计)。Phase B: Glycerin 60g, 1,3-Butanediol 20g, Polyethylene Glycol-63g, Xanthan Gum 1.5g, Hyaluronic Acid 0.5g, Methylparaben 2g, the balance is deionized water (based on 1000g ).
C相:神经酰胺0.2g,红葱乙酸乙酯提取物45g,仙人掌提取物20g,积雪草提取物20g。Phase C: 0.2g ceramide, 45g ethyl acetate extract of red onion, 20g cactus extract, 20g centella asiatica extract.
D相:苯氧乙醇3g,香精0.1g。Phase D: 3g of phenoxyethanol, 0.1g of essence.
制备:将A相加热到85℃,使其完全溶解,将B相加热至85℃,搅拌分散均匀,将A,B相混合均质并搅拌,当温度降到40℃以下时,加入C相、D相等并搅拌20分钟即得。Preparation: Heat phase A to 85°C to dissolve completely, heat phase B to 85°C, stir and disperse evenly, mix phases A and B homogeneously and stir, when the temperature drops below 40°C, add phase C , D are equal and stirred for 20 minutes.
实施例5红葱提取物护肤乳液1的制备The preparation of embodiment 5 shallot extract skin care lotion 1
A相:鲸蜡醇和鲸蜡硬脂基葡糖苷25g,辛酸/葵酸甘油三酯30g,环五聚二甲基硅氧烷20g,山梨坦硬脂酸酯10g,角鲨烷20g,羧甲基葡聚糖15g,环甲基硅氧烷15g,三甲基甘氨酸10g,月桂醇聚醚2g,PEG-40氢化蓖麻油8g,丁羟甲苯3g,。Phase A: Cetyl Alcohol and Cetearyl Glucoside 25g, Caprylic/Capric Triglyceride 30g, Cyclopentasiloxane 20g, Sorbitan Stearate 10g, Squalane 20g, Carboxymethyl 15g dextran, 15g cyclomethicone, 10g trimethylglycine, 2g laureth, 8g PEG-40 hydrogenated castor oil, 3g butylated hydroxytoluene.
B相:1,3丁二醇30g,丙二醇20g,透明质酸0.5g,尿囊素1g,羟苯甲酯2g,余量为去离子水(以1000g计)。Phase B: 30g of 1,3-butanediol, 20g of propylene glycol, 0.5g of hyaluronic acid, 1g of allantoin, 2g of methylparaben, and the balance is deionized water (based on 1000g).
C相:红葱乙酸乙酯提取物40g,仙人掌提取物20g,积雪草提取物20g。Phase C: 40g of ethyl acetate extract of red onion, 20g of cactus extract, 20g of centella asiatica extract.
D相:苯氧乙醇4g,香精0.2g。Phase D: 4g of phenoxyethanol, 0.2g of essence.
制备:将A相加热到85℃,使其完全溶解,将B相加热至85℃,搅拌分散均匀,将A,B相混合均质并搅拌,当温度降到40℃以下时,加入C相、D相等并搅拌20分钟即得。Preparation: Heat phase A to 85°C to dissolve completely, heat phase B to 85°C, stir and disperse evenly, mix phases A and B homogeneously and stir, when the temperature drops below 40°C, add phase C , D are equal and stirred for 20 minutes.
实施例6红葱提取物护肤乳液2的制备The preparation of embodiment 6 shallot extract skin care lotion 2
A相:氢化卵磷脂20g,鲸蜡醇和鲸蜡硬脂基葡糖苷10g,辛酸/葵酸甘油三酯35g,环五聚二甲基硅氧烷30g,单硬脂酸甘油酯15g,羧甲基葡聚糖20g,环甲基硅氧烷15g,三甲基甘氨酸10g,月桂醇聚醚2g,羟苯丙酯0.1g。Phase A: Hydrogenated Lecithin 20g, Cetyl Alcohol and Cetearyl Glucoside 10g, Caprylic/Capric Triglyceride 35g, Cyclopentasiloxane 30g, Glyceryl Monostearate 15g, Carboxymethyl Dextran 20g, Cyclomethicone 15g, Trimethylglycine 10g, Laureth 2g, Propylparaben 0.1g.
B相:1,3丁二醇30g,十六醇15g,透明质酸1g,尿囊素1g,羟苯甲酯0.5g,余量为去离子水(以1000g计)。Phase B: 30g of 1,3-butanediol, 15g of cetyl alcohol, 1g of hyaluronic acid, 1g of allantoin, 0.5g of methylparaben, and the balance is deionized water (based on 1000g).
C相:红葱乙酸乙酯提取物50g,仙人掌提取物20g,积雪草提取物30g,生育酚乙酸酯5g。Phase C: 50g ethyl acetate extract of red onion, 20g cactus extract, 30g centella asiatica extract, 5g tocopheryl acetate.
D相:苯氧乙醇5g,香精0.3g。Phase D: 5g of phenoxyethanol, 0.3g of essence.
制备:将A相加热到85℃,使其完全溶解,将B相加热至85℃,搅拌分散均匀,将A,B相混合均质并搅拌,当温度降到40℃以下时,加入C相、D相等并搅拌20分钟。Preparation: Heat phase A to 85°C to dissolve completely, heat phase B to 85°C, stir and disperse evenly, mix phases A and B homogeneously and stir, when the temperature drops below 40°C, add phase C , D are equal and stirred for 20 minutes.
实施例7红葱提取物面膜1的制备The preparation of embodiment 7 shallot extract facial mask 1
A相:1,3丁二醇50g,丙二醇20g,聚乙烯醇10g,汉生胶1.5g,透明质酸0.5g,尿囊素1g。Phase A: 50g of 1,3-butylene glycol, 20g of propylene glycol, 10g of polyvinyl alcohol, 1.5g of xanthan gum, 0.5g of hyaluronic acid, 1g of allantoin.
B相:生育酚乙酸酯4g,双丙甘醇10g,红葱乙酸乙酯提取物30g,仙人掌提取物10g,积雪草提取物15g,苯氧乙醇2g,羟苯甲酯0.5g,香精0.4g,其余为去离子水(以1000g计)。Phase B: 4g tocopheryl acetate, 10g dipropylene glycol, 30g ethyl acetate extract of red onion, 10g cactus extract, 15g centella asiatica extract, 2g phenoxyethanol, 0.5g methylparaben, essence 0.4g, and the rest is deionized water (based on 1000g).
制备:A相在85℃的条件下加热30min,再在一定转速的条件下搅拌,待温度冷却至40℃以下时,依次加入B相,直至搅拌均匀,把精华液灌入装有面膜纸的面膜袋中,待精华液充分浸入面膜纸,封口即得到含天然成分的面膜。Preparation: Heat phase A at 85°C for 30 minutes, and then stir at a certain speed. When the temperature cools down to below 40°C, add phase B in turn until it is evenly stirred, and pour the essence into the container with mask paper In the mask bag, after the essence is fully immersed in the mask paper, seal it to get a mask containing natural ingredients.
实施例8红葱提取物面膜2的制备The preparation of embodiment 8 shallot extract facial mask 2
A相:甘油40g,丙二醇30g,卡波姆940 5g,蔗糖2g,尿囊素1.5g。Phase A: glycerin 40g, propylene glycol 30g, carbomer 940 5g, sucrose 2g, allantoin 1.5g.
B相:三乙醇胺2g,PEG-40氢化蓖麻油2g,生育酚乙酸酯4g,红葱乙酸乙酯提取物20g,仙人掌提取物15g,积雪草提取物10g,苯氧乙醇2g,羟苯甲酯0.5g,香精0.2g,其余为去离子水(以1000g计)。Phase B: 2g triethanolamine, 2g PEG-40 hydrogenated castor oil, 4g tocopheryl acetate, 20g ethyl acetate extract of shallot, 15g cactus extract, 10g centella asiatica extract, 2g phenoxyethanol, hydroxybenzene 0.5g of methyl ester, 0.2g of essence, and the rest is deionized water (based on 1000g).
制备:A相在85℃的条件下加热30min,再在一定转速的条件下搅拌,待温度冷却至40℃以下时,依次加入B相,直至搅拌均匀,把精华液灌入装有面膜纸的面膜袋中,待精华液充分浸入面膜纸,封口即得到含天然成分的面膜。Preparation: Heat phase A at 85°C for 30 minutes, and then stir at a certain speed. When the temperature cools down to below 40°C, add phase B in turn until it is evenly stirred, and pour the essence into the container with mask paper In the mask bag, after the essence is fully immersed in the mask paper, seal it to get a mask containing natural ingredients.
实施例9红葱提取物精华液1的制备The preparation of embodiment 9 shallot extract essence 1
A相:角鲨烷20g,杏仁油30g,异十六烷30g,鲸蜡硬脂基葡糖苷20g,羟苯丙酯0.5g。Phase A: 20g squalane, 30g almond oil, 30g isohexadecane, 20g cetearyl glucoside, 0.5g propylparaben.
B相:甘油60g,双丙甘醇30g,尿囊素1g,EDTA二钠0.2g,羟苯甲酯1g,其余为去离子水(以1000g计)。Phase B: 60g of glycerin, 30g of dipropylene glycol, 1g of allantoin, 0.2g of disodium EDTA, 1g of methylparaben, and the rest is deionized water (calculated in 1000g).
C相:L-吡咯烷酮羧酸钠2g,红葱乙酸乙酯提取物40g,仙人掌提取物20g,积雪草提取物10g,丁羟甲苯2g,苯氧乙醇4g,香精0.3g。Phase C: 2g sodium L-pyrrolidone carboxylate, 40g ethyl acetate extract of shallot, 20g cactus extract, 10g centella asiatica extract, 2g butylated hydroxytoluene, 4g phenoxyethanol, 0.3g essence.
制备:A,B相在85℃的条件下加热30min,趁热均质3min,再在一定转速的条件下搅拌,待温度冷却至40℃以下时,依次加入C相,直至搅拌均匀。Preparation: Heat phases A and B at 85°C for 30 minutes, homogenize while hot for 3 minutes, and then stir at a certain speed. When the temperature is cooled below 40°C, add phase C in sequence until the mixture is uniform.
实施例10红葱提取物精华液2的制备The preparation of embodiment 10 shallot extract essence 2
A相:1,3丁二醇20g,双丙甘醇30g,卡波姆9405g,羟乙基纤维素5g,甘草酸二钾1g。Phase A: 20g of 1,3-butylene glycol, 30g of dipropylene glycol, 9405g of carbomer, 5g of hydroxyethyl cellulose, 1g of dipotassium glycyrrhizinate.
B相:三乙醇胺4g,生育酚乙酸酯5g,红葱乙酸乙酯提取物60g,仙人掌提取物15g,积雪草提取物10g,苯氧乙醇4g,羟苯甲酯1g,香精0.1g,其余为去离子水(以1000g计)。Phase B: 4g triethanolamine, 5g tocopheryl acetate, 60g ethyl acetate extract of red onion, 15g cactus extract, 10g centella asiatica extract, 4g phenoxyethanol, 1g methylparaben, 0.1g essence, The rest is deionized water (based on 1000g).
制备:A相在85℃的条件下加热30min,再在一定转速的条件下搅拌,待温度冷却至40℃以下时,依次加入B相,直至搅拌均匀。Preparation: Heat phase A at 85°C for 30 minutes, and then stir at a certain speed. When the temperature cools down to below 40°C, add phase B in sequence until the mixture is evenly stirred.
实施例11红葱乙酸乙酯提取部位物的抑菌和抗氧化活性测定Example 11 Bacteriostasis and Antioxidant Activity Determination of Ethyl Acetate Extracted Parts of Red Onion
对红葱乙酸乙酯提取部位物的抑菌和抗氧化活性进行测定,以金黄色葡萄球菌ATCC25923、大肠杆菌ATCC25922、鼠伤寒沙门氏菌ATCC14028、表皮金黄色葡萄球菌ATCC11827、痤疮丙酸杆菌ATCC6919为供试菌,测定红葱乙酸乙酯提取部位的6个质量浓度对供试菌的抑菌活性,结果表明,红葱乙酸乙酯提取部位对金黄色葡萄球菌和表皮金黄色葡萄球菌抑制活性最强,质量浓度为50mg/mL时,抑菌圈直径分别为27mm和26mm,其次是沙门氏菌,最大抑菌圈直径为16mm,对大肠杆菌和痤疮丙酸杆菌抑菌能力最弱(见图1~5)。The antibacterial and antioxidative activities of the extraction parts of shallot ethyl acetate were determined, using Staphylococcus aureus ATCC25923, Escherichia coli ATCC25922, Salmonella typhimurium ATCC14028, Staphylococcus epidermidis ATCC11827, Propionibacterium acnes ATCC6919 for the test Bacteria, and the antibacterial activity of 6 mass concentrations of the ethyl acetate extraction parts of shallots was measured against the tested bacteria. The results showed that the ethyl acetate extraction parts of red onions had the strongest inhibitory activity against Staphylococcus aureus and Staphylococcus epidermidis, When the mass concentration is 50mg/mL, the diameters of the inhibition zone are 27mm and 26mm respectively, followed by Salmonella, the maximum inhibition zone diameter is 16mm, and the antibacterial ability to Escherichia coli and Propionibacterium acnes is the weakest (see Figure 1-5) .
实施例12红葱乙酸乙酯提取部位物的抗氧化作用Antioxidant effect of embodiment 12 shallot ethyl acetate extract parts
红葱提取物乙酸乙酯层、正丁醇层、氯仿层、乙醇提层、石油醚层5个不同极性部位均有抗氧化活性,不同极性部位的抗氧化作用不同,对不同类型的自由基有选择性作用。其中以乙酸乙酯相和正丁醇相活性较强,氯仿相次之,石油醚相和乙醇提取物的抗氧化活性低。总抗氧化力(TAC)强弱为乙酸乙酯层>正丁醇层>氯仿层>乙醇提层>石油醚层,乙酸乙酯层提取物在DPPH•清除体系中,质量浓度为0.04~0.4 mg/mL时,对DPPH•清除率达60%~88%,质量浓度为2~4 mg/mL时,对•OH清除率达73%~86%(见图6~7)。Five different polar parts of shallot extract, ethyl acetate layer, n-butanol layer, chloroform layer, ethanol layer, and petroleum ether layer, have antioxidant activity, and the antioxidative effects of different polar parts are different. Free radicals are selective. Among them, the ethyl acetate phase and n-butanol phase had the strongest activity, followed by the chloroform phase, and the antioxidant activity of the petroleum ether phase and ethanol extract was low. The strength of total antioxidant capacity (TAC) is ethyl acetate layer>n-butanol layer>chloroform layer>ethanol layer>petroleum ether layer. mg/mL, the scavenging rate of DPPH• reached 60%-88%, and when the mass concentration was 2-4 mg/mL, the scavenging rate of •OH reached 73%-86% (see Figure 6-7).
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CN109718122A (en) * | 2019-03-15 | 2019-05-07 | 派能生物科技(深圳)有限公司 | A kind of anti-oxidant lightening compositions of skin and preparation method thereof |
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