CN102771593A - Ampelopsis grossedentata preparation for reducing blood sugar, blood lipid and blood pressure and its preparation method - Google Patents

Abstract

Belonging to the technical field of traditional Chinese medicine and health care product processing, the invention relates to an Ampelopsis grossedentata preparation for reducing blood sugar, blood lipid and blood pressure and its preparation method. The preparation solves the problem of applying Ampelopsis grossedentata in reducing blood sugar, blood lipid and blood pressure, its effective component utilization rate is high, and the production process is simplified. The preparation method consists of: crushing Ampelopsis grossedentata tender branches and leaves, carrying out water extraction or alcohol extraction one to three times, conducting filtration, combining filtrates, adding an organic solvent for separation or directly conducting concentrating to obtain an extract, performing drying, adding auxiliary materials, mixing the materials, conducting granulation and tabletting, and implementing sugarcoating, thus obtaining tablets or capsules or soluble granules (granules) or other oral preparations.

Description

A kind of rattan tea preparation for lowering blood sugar, blood fat and blood pressure and preparation method thereof

technical field

The invention relates to a rattan tea preparation for lowering blood sugar, blood fat and blood pressure and a preparation method thereof, in particular to the extraction of active ingredients of rattan tea and a production method thereof, and belongs to the technical field of traditional Chinese medicine and health care product processing.

Background technique

Vine tea, commonly known as Maoyan berry tea, Duanwu tea, and vine tea (also known as mountain sweet tea, dragon beard tea), is a tender product of Ampelopsis grossedentata (Hand-Mazz) W.T.wang. stems and leaves. Slightly bitter and sweet, it produces body fluid and quenches thirst; its taste is sweet and cool, and has the functions of clearing away heat and detoxification, antibacterial and anti-inflammatory, expelling wind and dampness, strengthening bones and muscles, lowering blood fat, and protecting liver and liver. At present, the pharmacological experiments on rattan tea in the prior art have just entered the preliminary stage, and there have been research reports on lowering blood fat and blood sugar, but there is no research report on rattan tea lowering blood pressure, and there is no single application of rattan tea in the three lowering aspects. In the existing research and utilization, most of them are compound preparations, which are used in combination with other foods and traditional Chinese medicines. There is no unilateral use, and the use effect is not ideal, which increases the cost and uncontrollable ingredients; to have a curative effect on the disease, it needs Larger dosage, and longer onset time; the patent of the invention solves the problem of the single application of vine tea in lowering blood sugar, blood fat, and blood pressure, with low cost and good curative effect, and overcomes the low utilization degree of single vine tea The disadvantage of low rate.

Contents of the invention

Aiming at the above-mentioned defects in the existing technology, the present invention aims to provide an extraction method that uses vine tea alone, improves the utilization degree of vine tea, and has a high extraction rate of active ingredients, and a preparation and production method with good use effect.

In order to achieve the above object, the present invention adopts the following technical solutions:

Pulverize the twigs and leaves of vine tea, extract with water or ethanol, filter the water extract, combine the filtrate, add an organic solvent for separation and purification or concentrate it into an extract, add auxiliary materials, mix, granulate, and tablet, Coated with sugar, made into tablets or capsules or granules (granules) or other oral preparations. In the present invention, can be added to collect vine tea volatile oil, water extraction is carried out at room temperature or under heating and boiling conditions, the amount of water is at least able to cover the medicinal materials, ethanol extraction is carried out at room temperature, the number of extractions is at least one to three times, the amount of alcohol In order to at least cover the medicinal materials, there is no limit to the amount of ethanol. Absolute ethanol or various concentrations of ethanol are used for ethanol; chloroform, ethyl acetate, petroleum ether, etc. are used as organic solvents, and petroleum ether-30, petroleum ether, etc. can be used for petroleum ether. Ether-60 or Petroleum Ether-90.

The rattan tea lowering blood fat, lowering blood sugar, lowering blood pressure pharmacodynamics experiment, the rattan tea preparation of the present invention is carried out the research of lowering blood fat, lowering blood sugar and lowering blood pressure of animal rats carried out by Animal Experiment Center of Sun Yat-sen University School of Medicine, the result shows: Compared with the blank control group, the low, medium and high doses of the rattan tea preparation by intragastric administration can significantly reduce the blood fat, blood sugar and blood pressure of rats, and there is a statistically significant difference.

In the hypolipidemic test, the low, medium and high doses of the rattan tea preparation of the present invention all significantly reduce the levels of total cholesterol (TC) and triglyceride (TG) in mice compared with the blank control group. Statistically significant difference. Compared with the positive drug Di'ao Xinxuekang, the embodiment group can reduce the total cholesterol (TC) level and triglyceride (TG) level in rats, so that the blood lipids of rats are significantly reduced, and there is a statistically significant difference, which has obvious lipid-lowering effects. Effect.

In the hypoglycemic experiment of rattan tea preparation of the present invention on experimental animal mice, the low, medium and high doses of rattan tea preparation of the present invention have significantly reduced serum glucose levels in mice compared with the blank control group, Statistically significant difference. Compared with the positive control drug metformin hydrochloride, the rattan tea water extract dosage group has an inhibitory effect on the increase of blood sugar in alloxan diabetic mice, and has an obvious hypoglycemic effect.

In the blood pressure lowering experiment, the vine tea extract extract of the present invention and the extract extract obtained by separating various organic solvents, intragastric administration, compared with the blank control group have a significant effect on the tolerance time of stimulating the blood pressure in mice. Significantly prolonged, statistically significant difference. Compared with the positive drug control propranolol, the tolerance time is prolonged, the curative effect is more significant than that of the positive drug group propranolol, and it has obvious antihypertensive effect. Through the pharmacodynamic experiments of vine tea for lowering blood fat, blood sugar and blood pressure, it is revealed that the vine tea preparation of the present invention has pharmacological and pharmacological effects of lowering blood fat, blood sugar and blood pressure, and is better than the positive control.

Beneficial effects of the present invention:

The vine tea preparation obtained by the present invention contains 19 kinds of essential nutrients and trace elements for the human body, high in calcium, iron, and magnesium, and is effective for arrhythmia, myocardial ischemia, hypertension, coronary heart disease, atherosclerosis, etc. It has a good preventive effect on diseases and a good health care effect on osteoporosis; rich in dietary fiber, carotene and vitamin E, has a good preventive effect on cancer; high manganese content is very beneficial to longevity; Gram-negative bacteria, influenza A and B viruses all have inhibitory effects; the rattan tea preparation of the present invention: easy to use, can be completed by patients, small dosage, rapid curative effect, easy to carry, relatively stable quality, and its production method is simple , The extraction rate of active ingredients is high. The dosage of vine tea preparation for adults is equivalent to 3-6 grams of Chinese herbal medicines per time, 2-3 times each time. It has a good function of lowering blood sugar, blood fat and blood pressure.

Detailed ways

Example 1

Crush the twigs and leaves of rattan tea, pass through a 20-mesh sieve, add 100°C water to submerge the medicinal materials, extract twice, one hour each time, filter, collect volatile oil at the same time, combine the filtrate and volatile oil, add flavoring agents, add water to adjust the volume, and make it for oral administration liquid.

Example 2

Crush the young branches and leaves of vine tea, pass through a 20-mesh sieve, add water at 100°C to submerge the medicinal materials, extract three times, each time for 1 hour, filter, collect volatile oil at the same time, combine the filtrate, and evaporate the filtrate to obtain a brown-black extract, add auxiliary materials to the extract, After mixing, add the collected volatile oil, mix evenly, add an appropriate amount of binders well known in the art, granulate and dry, add lubricant, press into tablets with a tablet machine, coat with sugar, and pack separately to obtain vine tea Tablets; the prepared granules can be filled into capsules by adding an appropriate amount of lubricant; the granules can also be subpackaged to make granules.

Example 3

The twigs and leaves of vine tea were crushed, passed through a 40-mesh sieve, ultrasonically extracted three times with room temperature water for 30 minutes each time, filtered, the filtrate was combined, and the filtrate was evaporated by a rotary evaporator to obtain a brown-black extract. The extract is spray-dried into powder, passed through a 100-120 mesh sieve, packed into capsules, and subpackaged to obtain capsules.

Example 4

Crush the twigs and leaves of vine tea, without sieving, decocting in water at 60°C twice, each time for one day, filter, combine the filtrate, add chloroform to the filtrate for extraction, let stand, the filtrate is divided into two layers, the water layer and the chloroform layer, and remove the water layer to obtain a chloroform layer.

The chloroform is evaporated to obtain a dark green extract, which is then added to the collected volatile oil, mixed evenly, and dried in vacuum or under normal pressure (through a sieve of 80-100 mesh). Add appropriate concentration of ethanol to make a soft material, make granules, dry, add lubricating oil, mix well, compress into tablets with a tablet machine, coat sugar-coated, and pack separately to obtain sugar-coated tablets.

Example 5

Same as Example 4, the extraction solution is ethanol, and the organic solvent is changed to ethyl acetate, petroleum ether, etc.

Example 6

Pharmacological Study on Hypolipidemia of Rattan Tea Extract Preparation

1. Experimental animals: SD rats, half male and half male, clean standard, a total of 40 rats were randomly divided into 4 groups, blank group, model group, positive control group and test product group, 10 rats in each group. After the SD rats were continuously given high-fat diet for 14 days, 30% of the animals in each group were randomly selected to collect blood from the retroocular plexus, and blood lipid levels such as total cholesterol (TC) and triglyceride (TG) were detected. As a result, compared with the blank control group, the success rate of the hyperlipidemia model reached 99%, which proved that the model was successfully constructed. After excluding individual animals with low blood lipid levels, the lipid-lowering drug experiment was carried out.

2. Sample source and preparation:

(1) Example 1 rattan tea extract aqueous solution A: rattan tea extract extract aqueous solution A (corresponding to crude drug content 200 mg/ml, dosage equivalent to crude drug 2000 mg/kg body weight).

(2) Positive control group: Di'ao Xinxuekang was dissolved in 20 ml of normal saline to make a suspension with a concentration of 30 mg/ml and a dose of 30 mg/kg body weight.

(3) Physiological saline (sodium chloride injection), with a NaCl content of 0.9%.

3. Experimental method:

The administration began on the ^15th day of high-fat feed feeding, and each animal group in the experiment was administered orally by gavage. After taking the medicine, fasting and drinking for 8 hours). Blood was collected from all animals through the orbit, and serum was collected to detect the level of blood lipid (blood lipid after administration). The results are shown in Table 1.

4. Experimental results

According to statistics, the lipid-lowering effect of rattan tea extract is shown in the following table 1.

After a course of treatment of the vine tea water extract obtained in Example 1 of the present invention, the tested drugs all reduced the levels of total cholesterol (TC) and triglyceride (TG) at different levels. Compared with the high-fat model group and the vehicle control group, each test drug group can reduce the level of total cholesterol (TC) and triglyceride (TG) (P<0.05), and there is a statistically significant difference.

Table 1 Study on the efficacy of vine tea extracts in lowering blood lipids

与造模组比较P＜0.05，*P<0.05 compared with blank group,

Compared with the modeling group, P<0.05,

Example 7

Hypoglycemic Pharmacology Study of Rattan Tea Extract Preparation

1. Experimental animals: NIH mice, male, weighing 18.0-23.1g, clean standard, 40 in total. Randomly divided into 4 groups, 10 in each group. Set blank control, negative control, positive control and rattan tea water extract group.

2. Sample source and configuration:

(1) Example 1 rattan tea extract aqueous solution A: rattan tea extract extract aqueous solution A (corresponding to crude drug content 200 mg/ml, dosage equivalent to crude drug 2000 mg/kg body weight).

(2) Positive control group: metformin hydrochloride (product of Guizhou Tianan Pharmaceutical Co., Ltd.).

(3) Physiological saline (sodium chloride injection), with a NaCl content of 0.9%.

3. Experimental method: alloxan to diabetes model

Normal mice were injected with alloxan (72mg·kg ^-1 ) through the tail vein, and the serum glucose level was predicted 72 hours later. Those whose blood glucose level was above 16mmol·L ^-1 were selected for research. Mice were divided into normal control group, model group, metformin hydrochloride (150mg·kg ^-1 ) group, rattan tea water extract dose group (200mg·kg ^-1 ) group, intragastric administration once a day, control group and model group were given Equal volume of normal saline for 7 days. One hour after the last administration, blood was taken from the orbital venous plexus of the mice to measure the blood glucose level, and the results are shown in Table 2. The results showed that, compared with the model group, the rattan tea water extract dose group had an inhibitory effect on the increase of blood sugar in alloxan diabetic mice, and had an obvious hypoglycemic effect.

4. Experimental results:

According to statistics, the half cough time and antitussive effect of each dose group are shown in Table 2 below.

Table 2 The cough-relieving effect of rattan tea water extract

*Compared with the blank group, P<0.01

Example 8

Antihypertensive Pharmacology Study of Rattan Tea Extract Preparation

1. Experimental animals: Wistar rats, male, body weight (250±26.3) g.

2. Sample source and preparation:

(1) Example 1 rattan tea extract aqueous solution A: rattan tea extract extract aqueous solution A (corresponding to crude drug content 200 mg/ml, dosage equivalent to crude drug 2000 mg/kg body weight).

(2) Positive control group: propranolol (Shanxi Yunpeng Pharmaceutical Co., Ltd.).

(3) Physiological saline (sodium chloride injection), with a NaCl content of 0.9%.

3. Experimental method: thoracic artery isolated vascular tension research method

3.1. Preparation and stabilization of thoracic aortic ring

Rats were decapitated, and the thoracic aorta was quickly taken out by opening the chest, placed in KH solution at 4°C saturated with mixed gas (95% O ₂ and 5% CO ₂ ), washed until there was no blood, and the peripheral blood was carefully removed. Adipose tissue and connective tissue were cut into 2-3mm long vascular rings, and the vascular rings were placed in 10ml of KH solution (at a constant temperature of 37°C, and a mixed gas of 95% O ₂ and 5% CO ₂ was introduced continuously, In the constant temperature jacket bottle with pH=7.4), each blood vessel ring is penetrated by two triangular hooks through the lumen of the blood vessel ring, one end is fixed, and the other end is connected to the tension sensor, and the tension change is collected and recorded by the BL-420E biological function experiment system . The vascular ring was added with a resting tension of about 2g, and it was stable for 90 minutes. During this period, the liquid was replaced with KH solution pre-warmed to 37°C every 15 minutes. After the vascular ring is completely stable, the formal experiment can be carried out.

3.2. The effect of rattan tea extract on the tension of vascular rings pre-contracted by potassium chloride

After the vascular ring was stabilized, it was repeatedly stimulated with 60 mmol/L KCl 3 times to induce the maximum contraction range of the blood vessel. When the contraction range difference caused by 3 consecutive same stimulations was less than 10%, it was regarded as 100% contraction tension. Drugs were added to each group according to the concentration gradient.

4. Experimental results:

According to statistics, the blood pressure-lowering effect of vine tea extract is shown in Table 3 below.

Compared with the blank control group, the rattan tea extract extract of the present invention and the extracted extract obtained by separating various organic solvents, administered through stomach, have a significant prolongation compared with the blank control group. There are significant differences. Compared with the positive drug control propranolol, the tolerance time was prolonged, and the curative effect was more significant than that of the positive drug group propranolol.

Table 3. Antihypertensive effect of vine tea extract

The experiment of rattan tea preparation of the present invention on experimental animals shows that compared with the blank control group, rats' blood lipid, blood sugar and blood pressure can be significantly reduced by gavage administration of the rattan tea preparation of the present invention, and there is a statistically significant difference. In the blood lipid-lowering test, compared with the positive drug Di'ao Xinxuekang contrast, the embodiment group has significantly reduced the blood fat of rats, and there is a statistically significant difference; the effect of reducing blood fat in Example 6 is better than that of the positive control. After testing, the drug of the present invention Other embodiments show similar effects to embodiment 6.

According to the disclosure and teaching of the above-mentioned specification, those skilled in the art to which the present invention belongs can also make changes and modifications to the above-mentioned embodiment. Therefore, the present invention is not limited to the specific embodiments disclosed and described above, and some modifications and changes to the present invention should also fall within the protection scope of the claims of the present invention. In addition, although some specific terms are used in this specification, these terms are only for convenience of description and do not constitute any limitation to the present invention.

Claims (9)

1. vine tea preparation of hypoglycemic a, blood fat, blood pressure and preparation method thereof; It is characterized in that: the production method of vine tea preparation once may further comprise the steps: the vine tea browse is pulverized, and water is carried or alcohol extracting one to three time; Aqueous extract filters; Merging filtrate is condensed into medicinal extract, processes oral formulations by conventional method.

2. vine tea preparation of a kind of hypoglycemic, blood fat according to claim 1, blood pressure and preparation method thereof; It is characterized in that: vine tea preparation, its raw material are the browses of vine tea, with the water extract of vine tea or alcohol extract as active ingredient; Process pharmaceutical composition, formulation is a peroral dosage form.

3. pharmaceutical composition according to claim 2 is characterized in that: said composition is to add tablet, capsule, granule, oral liquid or the aerosol etc. that acceptable auxiliary is processed on the pharmacy with described active ingredient.

4. pharmaceutical composition according to claim 2 is characterized in that: said vine tea is meant it and just adopts browse or its browse through processing.

5. vine tea preparation of a kind of hypoglycemic, blood fat according to claim 1, blood pressure and preparation method thereof, it is characterized in that: said vine tea preparation raw material is the single of vine tea, or its composition.

6. vine tea preparation of a kind of hypoglycemic, blood fat according to claim 1, blood pressure and preparation method thereof is characterized in that: behind the merging filtrate, add organic solvent extraction, reclaim organic solvent, get organic solvent layer, be condensed into medicinal extract.

7. vine tea preparation according to claim 6 is characterized in that: after being condensed into medicinal extract, add flavouring, add the water pondage, process oral liquid.

8. vine tea preparation according to claim 6 is characterized in that: after being condensed into medicinal extract, add auxiliary material, mix, add the volatile oil of having collected, granulate, add the conventional auxiliary material compressing tablet of tablet, process tablet.

9. vine tea preparation of a kind of hypoglycemic, blood fat according to claim 1, blood pressure and preparation method thereof, it is characterized in that: this oral formulations can be health products or medicine.