CN104887835A - Healthcare product prepared through natural plants - Google Patents

Abstract

The invention discloses a healthcare product prepared through natural plants, and belongs to the technical field of healthcare products. The healthcare product is prepared by evenly mixing Chinese torreya leaf extract, okra extract, balsam pear extract, lotus leaf extract, green tea extract, bee milk freeze-dried powder and selenium enriched spirulina. According to the composition ratio, 25 g to 40 g of the Chinese torreya leaf extract, 15 g to 30 g of the okra extract, 10 g to 20 g of the balsam pear extract, 5 g to 20 g of the lotus leaf extract, 10 g to 40 g of the green tea extract, 5 g to 10 g of the bee milk freeze-dried powder and 200 [mu]g to 800 [mu]g of the selenium enriched spirulina are included in every 100 g of the mixtures. The healthcare product prepared through the natural plants has the strong functions of resisting fatigue, enhancing human immunity and reducing blood glucose and blood fat; the healthcare effect is obvious, and the source of the raw materials is natural; the healthcare product is safe and free of side effects; the production process is simple, using is convenient, and storage is easy.

Description

A health product prepared from natural plants

technical field

The invention belongs to the technical field of health products, in particular to a health product prepared from natural plants.

Background technique

Among the top ten causes of death in China, the mortality rate related to metabolic diseases is as high as 35.7%, and the number of deaths related to the "three highs" also accounts for 27% of the total number of deaths. With the improvement of living standards and changes in the pace of life, the "three highs" (i.e. high blood pressure, high blood sugar and high blood fat) known as "rich man's disease" have entered the homes of ordinary people, especially middle-aged and elderly people over 50 years old. common disease. The number of people who die from cardiovascular and cerebrovascular diseases is as high as 15 million every year in the world, ranking first among various causes of death. Cardiovascular and cerebrovascular diseases have become the "number one killer" with the highest cause of death in humans. Cardiovascular and cerebrovascular diseases have the characteristics of "high morbidity, high disability rate, high mortality rate, high recurrence rate, and many complications. Hyperlipidemia can cause vascular embolism, and hyperglycemia can cause diabetes. The World Health Organization has clearly stated that preventing The first line of defense for cardiovascular disease is to reduce the "three highs" and control the "three highs". Although there are many drugs for lowering blood sugar and lipids in the market, most of them are western medicines or single traditional Chinese medicines. At present, natural plants that are used as food and medicine As a raw material, reducing the symptoms of patients with three highs through scientific and reasonable proportioning is the focus of people's attention.

Torreya is an evergreen tree plant belonging to the yew family Torreya genus. It is a precious and economical dried fruit tree species unique to my country. Torreya is also known as Torreya chinensis, Torreya true, and Torreya maple. There are 7 species of Torreya, among which Torreya is the one with the highest economic value. kind. Torreya torreya is full of treasures. It is a multi-purpose economic tree species integrating fruit, oil, medicine, wood, greening and ornamental. The leaves of Torreya torreya also have high nutritional value, which has not been fully utilized, resulting in a lot of waste. Therefore, in order to make the torreya industry bigger and stronger, it is necessary not only to provide guidance on planting and cultivation techniques, to ensure high yield and high yield, and to do a good job in brand effect, but also to carry out intensive technical processing of torreya fruit, leaves, arils and other parts and a series of products The development of Chinese torreya series products is made to form an industrial chain.

Okra is an annual herb belonging to Malvaceae okra. Okra has been designated as the first choice vegetable for athletes and health food for the elderly in many countries. It has the reputation of "plant Viagra" and "green ginseng". It is an excellent dietary and health vegetable. Okra contains flavonoids, polysaccharides, pectin, trace elements, amino acids and other functional ingredients, which have anti-fatigue, enhance human immunity, protect liver and kidney, anti-aging, help digestion, clear fire and eyesight, strengthen stomach and moisten intestines, etc. side effect. Okra is more and more popular because of its good functions and health care effects.

Bitter gourd, also known as bitter gourd, is a Cucurbitaceae plant and an annual climbing herb. Stems, branches, petioles and pedicels covered with pilose, axillary tendrils. Bitter gourd is a kind of vegetable that people like, and its origin is China. It is widely planted in Southeast Asia, China, and the Caribbean islands. Flavonoids extracted from bitter gourd and deoxynojirimycin extracted from mulberry leaves are combined to form a new substance, glucagon, which has the effects of lowering blood sugar, blood fat, and anti-inflammation.

The lotus leaf is a perennial herbaceous emergent plant of the lotus family, which has the effects of clearing the heart and relieving heat, dispelling blood stasis and stopping bleeding, and eliminating wind and dampness. Lotus leaf is a food for both medicine and food. It is rich in flavonoids, which are scavengers of most oxygen free radicals, can improve the activity of SOD, reduce lipid peroxide malondialdehyde and oxidize low-density lipoprotein It can increase the flow of coronary arteries, and has a protective effect on experimental myocardial infarction; it has a protective effect on acute myocardial ischemia; alkaloids in lotus leaves have significant physiological activities, and have obvious effects of lowering blood fat and anti-virus. Therefore, lotus leaf can be widely used in functional food and health food.

Green tea leaves are perennial evergreen woody plants of the family Theaceae Camellia. Tea polyphenols in green tea play an important role in human fat metabolism. The content of cholesterol and triglycerides in the human body is high, fat deposits on the inner wall of blood vessels, and cardiovascular diseases such as atherosclerotic plaques are formed after the proliferation of vascular smooth muscle cells. Tea polyphenols, especially the catechins ECG and EGC and their oxidation products theaflavins in tea polyphenols, help to inhibit this plaque hyperplasia, reduce the fibrinogen that forms blood coagulation viscosity, and make blood coagulation clearer. , thereby lowering blood lipids and inhibiting atherosclerosis. Tea polyphenols have a strong inhibitory effect on conversion enzyme activity, so they can lower or maintain blood pressure stability. Tea polyphenols have a regulating effect on the glucose metabolism disorder of the human body, and can lower blood sugar levels, thereby effectively preventing and treating diabetes. Tea polyphenols are new functional health products in tea that have the functions of anti-oxidation, anti-fatigue, anti-lipid peroxidation, anti-aging, scavenging free radicals, lowering blood fat and blood cholesterol, antibacterial and anti-inflammatory, anti-virus, and enhancing immune function. .

With the advancement of science and technology, more and more attention has been paid to the role of trace element selenium on human health. Organic selenium can be widely used in the production of selenium-enriched foods. It plays a certain role in anti-aging, prolonging life, preventing and treating cancer, myopia, cataract, cardiovascular and cerebrovascular diseases, endemic diseases and other diseases. In food and production, organic selenium products have been widely used as supplements of trace element selenium. At present, the production and application of trace element preparations such as selenium in some developed countries have reached a considerable scale. The nutritional and health value of organic selenium products, including selenium-enriched spirulina and selenium yeast, is hard to replace in the same industry.

Contents of the invention

Aiming at the above-mentioned problems existing in the prior art, the object of the present invention is to design and provide a technical scheme of a health care product prepared from natural plants, which has strong anti-fatigue, enhanced human immunity, hypoglycemic and lipid-lowering effects, and provides health benefits. The effect is obvious, the source of raw materials is natural, safe and has no side effects, and the production process is simple, convenient to use and easy to store.

The health product prepared from a natural plant is characterized in that it is prepared by mixing Torreya torreya leaf extract, okra extract, bitter melon extract, lotus leaf extract, green tea extract, royal jelly freeze-dried powder, and selenium-enriched spirulina. The composition ratio is: 25-40 g of Torreya leaf extract, 15-30 g of okra extract, 10-20 g of bitter melon extract, 5-20 g of lotus leaf extract, and 10 g of green tea extract in every 100 g of the mixture. -40 g, royal jelly freeze-dried powder 5-10 g, selenium-enriched spirulina 200-800 ;

The described torreya leaf extract is prepared by the following method: put the dried torreya leaves in the container of the broken extractor, add 60-80% ethanol according to the ratio of 6-8 liters of water-containing ethanol according to the dry weight of the torreya leaves, Start the switch, and carry out tissue crushing and extraction at room temperature at a speed of 10000-12000r/min for 2-3 times, breaking the raw materials into a particle size that can pass through a 60-100 mesh sieve, and each extraction time is 2-3 minutes; pump the extract Filtrate, discard the filter residue, and combine the obtained extracts at 60-65 ° C and a vacuum of 0.092-0.095 Mpa to flash and concentrate to 1/8-1/10 of the original volume with a vacuum thin-film concentration device, and distill the concentrated solution Transfer to a rotary evaporator, continue vacuum rotary evaporation to concentrate to a dry powder, and use a mortar to grind to obtain a dry powder of Torreya leaf extract, and the yield of the extract is 15%-26%;

The okra extract is prepared by the following method: take the okra fruit after low-temperature drying, cut it into sections, add 50-80% ethanol for ultrasonic extraction according to the ratio of 5-8 liters of water-containing ethanol according to the weight of the okra fruit 1 kg -4 times, each time for 30-60 minutes, suction filtration, discarding the filter residue, and combining the extracts at 60-70°C and vacuum of 0.092-0.095 Mpa, use a vacuum film concentration device to flash and concentrate to the original volume 1/6-1/10, transfer the concentrated solution to a rotary evaporator, continue vacuum rotary evaporation to concentrate to a dry powder, and grind it with a mortar to obtain a dry powder of okra extract. The yield of the extract is 15%-22 %;

The bitter gourd extract is prepared by the following method: take sliced and dried bitter gourd, extract by decocting, add water to decoct and extract 2-3 times according to the proportion of 1 kg of bitter gourd and the water consumption is 5-8 liters, each time After 30-40 hours, suction filtration, the obtained extract was flashed and concentrated to 1/8-1/10 of the original volume at 60°C-80°C and a vacuum of 0.093-0.095 Mpa, and the concentrated solution was transferred to a rotary In the evaporator, continue the reduced-pressure rotary evaporation to concentrate to a dry powder, and grind it with a mortar to obtain a dry powder of bitter melon extract, and the yield of the extract is 24%-35%;

The lotus leaf extract is prepared by the following method: put the dried lotus leaf in a flask, adopt the reflux extraction method, add 50%-70% of the water-containing ethanol according to the weight of the lotus leaf 1 kg, and the amount of water-containing ethanol is 6-10 liters Reflux extraction with water-containing ethanol for 2-3 times, each time for 1-2 hours, and suction filtration. The obtained extract is flashed and concentrated to 1/6 of the original volume at 50°C-70°C and a vacuum of 0.092-0.095 Mpa. -1/10, transfer the concentrated solution to a rotary evaporator, continue the vacuum rotary evaporation to concentrate to a dry powder, and grind it with a mortar to obtain a dry powder of lotus leaf extract. The yield of the extract is 18%-32%;

The green tea extract is prepared by the following method: put the dried green tea leaves in the container of the broken extractor, add 50%-75% acetone according to the ratio of 5-8 liters of water-containing acetone according to the weight of 1 kg of green tea leaves, and start Switch on and off, carry out room temperature tissue crushing and extraction at a speed of 10000-12000 r/min for 2-3 times, break the raw material into a particle size that can pass through a 60-100 mesh sieve, and extract for 2-5 minutes each time, pump the extract Filter, discard the filter residue, and combine the obtained extracts at 60-62°C and a vacuum of 0.093-0.095 Mpa to flash concentrate to 1/5-1/8 of the original volume using a vacuum thin film concentration device; add green tea leaves Activated carbon with an amount of 1-4% by weight is soaked and decolorized at 60-62°C for 30-60 minutes, and the concentrated solution after decolorization continues to be concentrated in vacuum until there is no acetone, and then extracted with ethyl acetate; each addition is equivalent to the volume of the concentrated solution Extract 1-2 times the amount of ethyl acetate for 3-6 times, combine the extracts, and use a rotary evaporator to continue vacuum concentration to dry powder under the condition of a water bath temperature of 60-62 ° C and a vacuum degree of 0.092-0.095 Mpa. The dry powder is scraped off the wall of the flask, ground into fine powder with a mortar to obtain the green tea extract, and the yield of the green tea polyphenol extract is 12-22%.

The health product prepared from a kind of natural plant is characterized in that in every 100 g of the mixture, there are 30-35 g of Torreya leaf extract, 20-25 g of okra extract, 12-18 g of bitter melon extract, and 8 g of lotus leaf extract. -15 g, green tea extract 20-30 g, royal jelly freeze-dried powder 6-8 g, selenium-enriched spirulina 400-500 g .

The health product prepared by a kind of natural plant is characterized in that in the preparation of the Torreya torreya leaf extract: the rotating speed of the crushing extractor is 11000r/min, and the raw material is broken into a particle size that can pass through a 70-85 mesh sieve, and the The vacuum thin film concentration device performs flash concentration to 1/9 of the original volume.

The health product prepared from natural plants is characterized in that in the preparation of the okra extract: flash concentration to 1/7-1/8 of the original volume.

The health product prepared from natural plants is characterized in that in the preparation method of the bitter gourd extract: decocting in water for 32-35 hours each time, flashing and concentrating to 1/9 of the original volume.

The health care product prepared from natural plants is characterized in that in the preparation method of the lotus leaf extract: flash concentration to 1/7-1/8 of the original volume.

The health product prepared from natural plants is characterized in that in the preparation method of the green tea extract: the rotating speed of the crushing extractor is 11000 r/min, and the raw material is crushed into a particle size that can pass through a 70-80 mesh sieve , using a vacuum thin film concentration device for flash concentration to 1/6-1/7 of the original volume; warm soaking for 40-50 minutes to decolorize.

The above-mentioned royal jelly freeze-dried powder is obtained by drying under low-temperature vacuum, its nutritional components are not destroyed, it is convenient to take, and easy to store; the main components are vitamins, tocopherols, minerals, amino acids, and active enzymes; Anti-fatigue, anti-aging, lowering blood sugar, enhancing immune function, mediating endocrine, preventing cardiovascular and cerebrovascular diseases, etc.

The above-mentioned selenium-enriched spirulina contains organic element selenium and spirulina, has anti-aging effects, and is used for preventing and treating tumors, cardiovascular and cerebrovascular diseases, and the like.

The above-mentioned health products can be directly sealed and packed in bags, or can be made into granules, pills, and capsules for easy use.

The health product prepared by the above-mentioned natural plant has the following advantages:

1. The crushing extractor used in the present invention uses advanced crushing and mixing technology to crush and mix the raw materials. After high-speed crushing, stirring, mixing, emulsification, and homogenization, it is crushed into a homogenous slurry to achieve uniform mixing and stirring at room temperature. , the extraction speed is fast, complete, high extraction rate, high efficiency, energy saving, and high efficiency.

2. Most of the raw materials used in the present invention are health-care vegetables or natural plants that can be used as both medicine and food. They have strong nutritional and health value and auxiliary therapeutic effects. They are pure natural green foods with outstanding efficacy, safe use, and environmental protection. Product development can improve the utilization rate of deep processing of agricultural products, which is in line with my country's current "three rural" policy. The method can realize industrialized production and improve resource utilization.

3. The preparation method of the present invention has simple production process and low cost. The prepared natural active substance has the characteristics of high health-care nutritional value, significant efficacy, convenient consumption, easy absorption, safety and no side effects, etc., and has strong effects of anti-fatigue, anti-aging, enhancing immunity, and lowering blood sugar and fat. Long-term use not only has a good health care effect, but also has a good therapeutic effect. Moreover, the composition is reasonable, has synergistic effects, is convenient to use, and has a simple production process, can realize industrialized production, and improves the added value of various natural plants and the utilization rate of resources.

Unless otherwise specified, the percentages involved in this patent application are the weight percentages of pure substances of active ingredients.

Detailed ways

The beneficial effects of the present invention are further described below in conjunction with specific examples and corresponding test data.

Example 1

The health care product is made by mixing Chinese torreya leaf extract, okra extract, bitter melon extract, lotus leaf extract, green tea extract, royal jelly freeze-dried powder, and selenium-enriched spirulina. Torreya leaf extract 40 g, okra extract 15 g, bitter melon extract 10 g, lotus leaf extract 5 g, green tea extract 25 g, royal jelly freeze-dried powder 5 g, selenium-enriched spirulina 500 g composition;

The described torreya leaf extract is prepared by the following method: put the dried torreya leaves in the container of the broken extractor, add 60-80% ethanol according to the ratio of 6-8 liters of water-containing ethanol according to the dry weight of the torreya leaves, Start the switch, and carry out tissue crushing and extraction at room temperature at a speed of 10000-12000r/min for 2-3 times, breaking the raw materials into a particle size that can pass through a 60-100 mesh sieve, and each extraction time is 2-3 minutes; pump the extract Filtrate, discard the filter residue, and combine the obtained extracts at 60-65 ° C and a vacuum of 0.092-0.095 Mpa to flash and concentrate to 1/8-1/10 of the original volume with a vacuum thin-film concentration device, and distill the concentrated solution Transfer to a rotary evaporator, continue the reduced-pressure rotary evaporation to concentrate to a dry powder, and grind it with a mortar to obtain a dry powder of Torreya leaf extract;

The okra extract is prepared by the following method: take the okra fruit after low-temperature drying, cut it into sections, add 50-80% ethanol for ultrasonic extraction according to the ratio of 5-8 liters of water-containing ethanol according to the weight of the okra fruit 1 kg -4 times, each time for 30-60 minutes, suction filtration, discarding the filter residue, and combining the extracts at 60-70°C and vacuum of 0.092-0.095 Mpa, use a vacuum film concentration device to flash and concentrate to the original volume 1/6-1/10, transfer the concentrated solution to a rotary evaporator, continue vacuum rotary evaporation to concentrate to a dry powder, and grind it with a mortar to obtain a dry powder of okra extract;

The bitter gourd extract is prepared by the following method: take sliced and dried bitter gourd, extract by decocting, add water to decoct and extract 2-3 times according to the proportion of 1 kg of bitter gourd and the water consumption is 5-8 liters, each time After 30-40 hours, suction filtration, the obtained extract was flashed and concentrated to 1/8-1/10 of the original volume at 60°C-80°C and a vacuum of 0.093-0.095 Mpa, and the concentrated solution was transferred to a rotary In the evaporator, continue the reduced-pressure rotary evaporation to concentrate to a dry powder, and grind it with a mortar to obtain a dry powder of bitter melon extract;

The lotus leaf extract is prepared by the following method: put the dried lotus leaf in a flask, adopt the reflux extraction method, add 50%-70% of the water-containing ethanol according to the weight of the lotus leaf 1 kg, and the amount of water-containing ethanol is 6-10 liters Reflux extraction with water-containing ethanol for 2-3 times, each time for 1-2 hours, and suction filtration. The obtained extract is flashed and concentrated to 1/6 of the original volume at 50°C-70°C and a vacuum of 0.092-0.095 Mpa. -1/10, transfer the concentrated solution to a rotary evaporator, continue the vacuum rotary evaporation to concentrate to a dry powder, and grind it with a mortar to obtain a dry powder of lotus leaf extract;

The green tea extract is prepared by the following method: put the dried green tea leaves in the container of the broken extractor, add 50%-75% acetone according to the ratio of 5-8 liters of water-containing acetone according to the weight of 1 kg of green tea leaves, and start Switch on and off, carry out room temperature tissue crushing and extraction at a speed of 10000-12000 r/min for 2-3 times, break the raw material into a particle size that can pass through a 60-100 mesh sieve, and extract for 2-5 minutes each time, pump the extract Filter, discard the filter residue, and combine the obtained extracts at 60-62°C and a vacuum of 0.093-0.095 Mpa to flash concentrate to 1/5-1/8 of the original volume using a vacuum thin film concentration device; add green tea leaves Activated carbon with an amount of 1-4% by weight is soaked and decolorized at 60-62°C for 30-60 minutes, and the concentrated solution after decolorization continues to be concentrated in vacuum until there is no acetone, and then extracted with ethyl acetate; each addition is equivalent to the volume of the concentrated solution Extract 1-2 times the amount of ethyl acetate for 3-6 times, combine the extracts, and use a rotary evaporator to continue vacuum concentration to dry powder under the condition of a water bath temperature of 60-62 ° C and a vacuum degree of 0.092-0.095 Mpa. The dry powder was scraped off the side of the flask and ground in a mortar to obtain the green tea extract.

Example 2

The health product is made by mixing Torreya torreya leaf extract, okra extract, bitter melon extract, lotus leaf extract, green tea extract, royal jelly freeze-dried powder, and selenium-enriched spirulina. Torreya leaf extract 30g, okra extract 20g, bitter gourd extract 10g, lotus leaf extract 10g, green tea extract 25g, royal jelly freeze-dried powder 5g, selenium-enriched spirulina 400g Composition; The preparation method of extract is the same as embodiment 1.

Example 3

The health care product is made by mixing Chinese torreya leaf extract, okra extract, bitter melon extract, lotus leaf extract, green tea extract, royal jelly freeze-dried powder, and selenium-enriched spirulina. Torreya leaf extract 25 g, okra extract 20 g, bitter melon extract 15 g, lotus leaf extract 10 g, green tea extract 20 g, royal jelly freeze-dried powder 10 g, selenium-enriched spirulina 300 g Composition; The preparation method of extract is the same as embodiment 1.

Example 4

The health care product is made by mixing Chinese torreya leaf extract, okra extract, bitter melon extract, lotus leaf extract, green tea extract, royal jelly freeze-dried powder, and selenium-enriched spirulina. Torreya leaf extract 25 g, okra extract 25 g, bitter melon extract 10 g, lotus leaf extract 10 g, green tea extract 25 g, royal jelly freeze-dried powder 5 g, selenium-enriched spirulina 600 Composition; The preparation method of extract is the same as embodiment 1.

Example 5

The health care product is made by mixing Chinese torreya leaf extract, okra extract, bitter melon extract, lotus leaf extract, green tea extract, royal jelly freeze-dried powder, and selenium-enriched spirulina. Torreya leaf extract 35 g, okra extract 15 g, bitter melon extract 10 g, lotus leaf extract 15 g, green tea extract 15 g, royal jelly freeze-dried powder 10 g, selenium-enriched spirulina 200 g Composition; The preparation method of extract is the same as embodiment 1.

Example 6

The health care product is made by mixing Chinese torreya leaf extract, okra extract, bitter melon extract, lotus leaf extract, green tea extract, royal jelly freeze-dried powder, and selenium-enriched spirulina. Torreya leaf extract 30 g, okra extract 15 g, bitter melon extract 10 g, lotus leaf extract 5 g, green tea extract 30 g, royal jelly freeze-dried powder 10 g, selenium-enriched spirulina 500 g Composition; The preparation method of extract is the same as embodiment 1.

Experiment 1: Anti-fatigue test in mice

Take clean male Kunming mice with a body weight of about 18-22 g, and inject 0.5 mg of dexamethasone sodium phosphate injection intramuscularly for 7 consecutive days to prepare an animal model of kidney yang deficiency. The mice were randomly divided into 6 groups, namely normal control group, model group, positive group, high-dose drug group (3.2g/kg), middle-dose group (1.6g/kg) and low-dose group (0.8g/kg ), 10 rats in each group, each group was orally administered the test drug at 0.2mL/10g, the normal control group and the model group were injected with the same dose of normal saline during the administration period, and the positive group was injected with the same dose of Red Bull drink during administration. Once a day, continuous administration for 15 days. Each group of mice was randomly divided into two groups, one group was used for weight-bearing swimming test, and the other group was used for the test of mouse liver glycogen, serum urea and blood lactic acid content.

Mouse weight-bearing swimming test: the animals were trained twice before formal swimming, and all mice were placed in the swimming box for forced swimming 30 minutes after the last administration. The base of the mouse tail was loaded with 5% lead skin of body weight. Record the time from the start of swimming to the time when the head is completely submerged in the water and cannot surface as the final exhaustive swimming time (s), see Table 1. Compared with the normal control group, the weight-bearing swimming time of the mice in the kidney-yang deficiency model group was significantly reduced. Compared with the model group, with the increase of the dosage, the weight-bearing swimming time of the mice gradually increased. Wherein the high-dose group of the embodiment prolongs the weight-bearing swimming time of the mice with deficiency of kidney yang more, indicating that Examples 1-6 have very good kidney-reinforcing and anti-fatigue effects.

Determination of liver glycogen: Blood sugar is the main source of energy for muscle activity. Prolonged intense exercise, physical exhaustion always occurs at the same time as the depletion of liver glycogen, and the storage of glycogen can directly affect the body's exercise capacity. Therefore, the content of glycogen can explain the speed or degree of fatigue. 30 minutes after the last administration, the mice were placed in a swimming tank at a water temperature of 25°C for 90 minutes without weight bearing, and immediately after swimming, they were sacrificed by cervical dislocation and the liver was taken out, and 100 mg of the liver was weighed. Visible spectrophotometer, using the classic anthrone method to determine the content of liver glycogen. The measurement results are shown in Table 2. Compared with the normal group, the liver glycogen content in the model group was significantly reduced. Compared with the model group, the liver glycogen content in each drug group was significantly higher, and the liver glycogen content also increased with the increase of the drug dose. It shows that the different dosage groups of Examples 1-6 all show a strong liver glycogen storage function, so they have a strong anti-fatigue effect.

Determination of serum urea: renal failure, nephritis, etc. can increase blood urea nitrogen content. After a week of intraperitoneal injection of a large amount of glucocorticoid, the model of kidney yang deficiency was prepared, and the serum urea value of the mice would naturally increase. With the administration of the drug, observe whether the serum urea of the mice decreases. 30 minutes after the last administration, the mice were placed in a swimming box with a water temperature of 25°C for 90 minutes without weight bearing, and after a 60-minute rest, the eyeballs were removed to collect blood, the serum was centrifuged, and the blood urea nitrogen (BUN) kit and microplate reader were used to measure the mice. Rat serum urea content, see Table 3.

Because a large amount of glucocorticoids were used in the modeling, a model of kidney yang deficiency was made, and renal failure can increase the blood urea nitrogen content. In Table 3, the urea nitrogen content in the model group was the highest, and the urea nitrogen could be excreted through the kidneys with the urine after administration, resulting in a significant decrease in the blood urea nitrogen content, and the reduction rate also increased with the increase of the drug dose, so The different dose groups of Examples 1-6 all showed obvious effects of improving renal function.

Determination of blood lactic acid: The determination of blood lactic acid is the most commonly used biochemical index to evaluate the degree of body fatigue during strenuous exercise. 30 minutes after the last administration, blood was collected from the vein, and then swimming in 30°C water without weight for 10 minutes was stopped. Blood was collected from mice before swimming, 0 minutes and 20 minutes after exercise, and serum was collected by centrifugation. Lactic acid test box and A microplate reader was used to measure blood lactate content LAC. Compared with the normal control group, the LAC of the yang-deficiency mice increased significantly after swimming, and the blood lactic acid concentration decreased very slowly after rest. In particular, the high-dose group can most significantly reduce the LAC value of the yang-deficiency mice after exercise, indicating that the different dose groups of Example 1 have obvious fatigue-relieving effects, as shown in Table 4.

Test 2: Rat hypoglycemic and lipid-lowering test

Sixty clean-grade male SD rats, each weighing 180-200 g, were fed for one week before the test began. In addition to the normal control group, alloxan (70 mg/kg) was injected intraperitoneally on an empty stomach once a day for 7 consecutive days. The normal control group was given an equal volume of normal saline. Blood sugar, to observe whether the modeling is successful. The successfully modeled rats were randomly divided into 5 groups (n=10), namely the model group, the metformin positive group (100 mg/kg), the drug high, medium and low dose groups (4000 mg/kg, 2000 mg/kg , 1000 mg/kg), orally administered for 15 consecutive days, the normal control group and the model group were given equal volumes of normal saline.

Determination of fasting blood glucose (FBG) in rats: After 7 days of modeling and at the end of the 14th day of drug treatment, fasting for 12 hours, and 2 hours after drug administration, blood was collected from the tail vein, serum was centrifuged, and glucose oxidase kit was used and ultraviolet-visible spectrophotometer to measure glucose content in fasting serum of rats, see Table 5. Before administration and treatment, the blood sugar of the rats in the model group was significantly increased, and compared with the control group, the blood sugar of all model rats was significantly higher than that of the control group. After two weeks of administration and treatment, the blood sugar of the rats in the administration group was significantly reduced, as shown in Table 5, wherein the blood sugar reduction of the high-dose group was more significant, showing that Examples 1-6 had obvious hypoglycemic effects on diabetic rats .

Determination of triglyceride and total cholesterol in rat serum: 7 days after modeling and at the end of the 14th day of drug administration, fasting for 12 hours, 2 hours after drug administration, anesthetized with chloral hydrate, decapitated, and blood was collected. The serum was separated, and the contents of triglyceride and total cholesterol in rat serum were determined using a triglyceride and total cholesterol kit and a microplate reader, see Table 6 and Table 7. Compared with the model group, the content of triglyceride and total cholesterol in the rat serum of the control group and the administration group were significantly reduced, especially the high-dose group could significantly reduce the content of cholesterol and triglyceride in the rat serum, indicating that the implementation of Examples 1-6 have good cholesterol-lowering and triglyceride-lowering effects on diabetic rats.

Through the observation of the weight and food intake of the rats, it was found that the weight and food intake of the rats after modeling had increased significantly, and symptoms of diabetic polydipsia, polyphagia, and polyuria appeared. The body weight and food intake of the rats were significantly improved, and the effect was the most significant in the high-dose group.

Claims (7)

1. A health product prepared from natural plants, characterized in that it is made by mixing Torreya torreya leaf extract, okra extract, bitter melon extract, lotus leaf extract, green tea extract, royal jelly freeze-dried powder, and selenium-enriched spirulina , and its composition ratio is: 25-40 g of Torreya leaf extract, 15-30 g of okra extract, 10-20 g of bitter melon extract, 5-20 g of lotus leaf extract, and 10-20 g of green tea extract in every 100 g of the mixture 40 g, royal jelly freeze-dried powder 5-10 g, selenium-enriched spirulina 200-800 The described torreya leaf extract is prepared by the following method: put the dried torreya leaves in the container of the broken extractor, add 60-80% ethanol according to the ratio of 6-8 liters of water-containing ethanol according to the dry weight of the torreya leaves, and start Switch on and off, carry out tissue crushing and extraction at room temperature for 2-3 times at a speed of 10000-12000r/min, break the raw material into a particle size that can pass through a 60-100 mesh sieve, and extract for 2-3 minutes each time; filter the extract , discard the filter residue, and combine the obtained extracts at 60-65°C and a vacuum of 0.092-0.095 Mpa to flash and concentrate to 1/8-1/10 of the original volume using a vacuum thin-film concentration device, and transfer the concentrated solution to In the rotary evaporator, continue the reduced-pressure rotary evaporation to concentrate to a dry powder, and grind it with a mortar to obtain a dry powder of Torreya leaf extract; The okra extract is prepared by the following method: take the okra fruit after low-temperature drying, cut it into sections, add 50-80% ethanol for ultrasonic extraction according to the ratio of 5-8 liters of water-containing ethanol according to the weight of the okra fruit 1 kg -4 times, each time for 30-60 minutes, suction filtration, discarding the filter residue, and combining the extracts at 60-70°C and vacuum of 0.092-0.095 Mpa, use a vacuum film concentration device to flash and concentrate to the original volume 1/6-1/10, transfer the concentrated solution to a rotary evaporator, continue vacuum rotary evaporation to concentrate to a dry powder, and grind it with a mortar to obtain a dry powder of okra extract; The bitter gourd extract is prepared by the following method: take sliced and dried bitter gourd, extract by decocting, add water to decoct and extract 2-3 times according to the proportion of 1 kg of bitter gourd and the water consumption is 5-8 liters, each time After 30-40 hours, suction filtration, the obtained extract was flashed and concentrated to 1/8-1/10 of the original volume at 60°C-80°C and a vacuum of 0.093-0.095 Mpa, and the concentrated solution was transferred to a rotary In the evaporator, continue the reduced-pressure rotary evaporation to concentrate to a dry powder, and grind it with a mortar to obtain a dry powder of bitter melon extract; The lotus leaf extract is prepared by the following method: put the dried lotus leaf in a flask, adopt the reflux extraction method, add 50%-70% of the water-containing ethanol according to the weight of the lotus leaf 1 kg, and the amount of water-containing ethanol is 6-10 liters Reflux extraction with water-containing ethanol for 2-3 times, each time for 1-2 hours, and suction filtration. The obtained extract is flashed and concentrated to 1/6 of the original volume at 50°C-70°C and a vacuum of 0.092-0.095 Mpa. -1/10, transfer the concentrated solution to a rotary evaporator, continue the vacuum rotary evaporation to concentrate to a dry powder, and grind it with a mortar to obtain a dry powder of lotus leaf extract; The green tea extract is prepared by the following method: put the dried green tea leaves in the container of the broken extractor, add 50%-75% acetone according to the ratio of 5-8 liters of water-containing acetone according to the weight of 1 kg of green tea leaves, and start Switch on and off, carry out room temperature tissue crushing and extraction at a speed of 10000-12000 r/min for 2-3 times, break the raw material into a particle size that can pass through a 60-100 mesh sieve, and extract for 2-5 minutes each time, pump the extract Filter, discard the filter residue, and combine the obtained extracts at 60-62°C and a vacuum of 0.093-0.095 Mpa to flash concentrate to 1/5-1/8 of the original volume using a vacuum thin film concentration device; add green tea leaves Activated carbon with an amount of 1-4% by weight is soaked and decolorized at 60-62°C for 30-60 minutes, and the concentrated solution after decolorization continues to be concentrated in vacuum until there is no acetone, and then extracted with ethyl acetate; each addition is equivalent to the volume of the concentrated solution Extract 1-2 times the amount of ethyl acetate for 3-6 times, combine the extracts, and use a rotary evaporator to continue vacuum concentration to dry powder under the condition of a water bath temperature of 60-62 ° C and a vacuum degree of 0.092-0.095 Mpa. The dry powder was scraped off the side of the flask and ground in a mortar to obtain the green tea extract. 2. a kind of health product prepared by natural plants as claimed in claim 1, is characterized in that in every 100 g mixture, Chinese torreya leaf extract 30-35 g, okra extract 20-25 g, balsam pear extract 12-18 g , lotus leaf extract 8-15 g, green tea extract 20-30 g, royal jelly freeze-dried powder 6-8 g, selenium-enriched spirulina 400-500 g . 3. The health care product prepared by a kind of natural plant as claimed in claim 1, it is characterized in that in the preparation of the torreya leaf extract: the rotating speed of the crushing extractor is 11000r/min, and the raw material is broken into pieces that can pass through 70-85 The particle size of the mesh sieve is flashed and concentrated to 1/9 of the original volume by using a vacuum thin film concentration device. 4. A health product prepared from natural plants as claimed in claim 1, characterized in that in the preparation of the okra extract: flash concentration to 1/7-1/8 of the original volume. 5. A health care product prepared from a natural plant as claimed in claim 1, characterized in that in the preparation method of the bitter gourd extract: decocting in water for 32-35 hours each time, flashing and concentrating to the original volume 1/9. 6. A health care product prepared from natural plants as claimed in claim 1, characterized in that in the preparation method of the lotus leaf extract: flash concentration to 1/7-1/8 of the original volume. 7. The health care product prepared by a kind of natural plant as claimed in claim 1, it is characterized in that in the preparation method of the green tea extract: the rotating speed of the crushing extractor is 11000 r/min, and the raw material is broken into pieces that can pass through 70 - The particle size of 80 mesh sieve, use the vacuum film concentration device to carry out flash evaporation concentration to 1/6-1/7 of the original volume; warm soak for 40-50 minutes to decolorize.