CN102302545A - Dai medicine extract with blood-sugar reducing activity, preparation method, composition and application - Google Patents
Dai medicine extract with blood-sugar reducing activity, preparation method, composition and application Download PDFInfo
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Abstract
本发明公开了从傣药猫须草（Clerodendranthusspicatus（Thunb.）C.Y.WuexH.W.Li）中提取的降血糖活性提取物，这种提取物的制备方法，含有猫须草（Clerodendranthusspicatus（Thunb.）C.Y.WuexH.W.Li）提取物的药物组合，以及猫须草提取物和其药物组合作为药物和保健品的应用，尤其是可用于制备治疗和预防糖尿病及其并发症等相关的代谢性疾病的药物和保健品。The invention discloses a hypoglycemic active extract extracted from Dai medicine Clerodendranthus spicatus (Thunb.) CYWuexH.W.Li, the preparation method of the extract contains Clerodendranthus spicatus (Thunb.) CYWuexH.W.Li .W.Li) extract's drug combination, and the application of cat's whiskers extract and its drug combination as medicine and health care products, especially for the preparation of drugs for the treatment and prevention of diabetes and its complications and other related metabolic diseases and health products.
Description
Technical field
The present invention relates to medicine Herba clerodendranthi spicati from the Dai Nationality
Clerodendranthus spicatus(Thunb.) extracting among the C. Y. Wu ex H. W. Li can hypoglycemic extract, and this preparation method of extract contains Herba clerodendranthi spicati
Clerodendranthus spicatus(Thunb.) pharmaceutical composition of C. Y. Wu ex H. W. Li extract, and Herba clerodendranthi spicati
Clerodendranthus spicatus(Thunb.) C. Y. Wu ex H. W. Li extract and medicine and the health product of its pharmaceutical composition as the treatment metabolic disease relevant with prevent diabetes and complication thereof etc.
Background technology
Diabetes are a kind of whole body chronic progressive diseases.Because the normal excreting insulin of islet cells, thereby insulin relatively or absolute deficiency, and target cell is to the reduction of insulin sensitivity, and cause sugar, albumen, fat and water, metabolic disturbance of electrolyte, and hepatic glycogen and muscle glycogen can not be synthesized.Clinical manifestation is blood sugar increasing, the glucose in urine positive, carbohydrate tolerance reduction etc.; Long-term ill meeting is in succession with a series of complication, because people's living standard raises, living habit changes; China's diabetics number constantly increases, and becomes to be only second to the second largest in the world country of India.
To the treatment of diabetes medicine, taboo and untoward reaction are more, take for a long time, cause liver toxicity and digestive tract side effects problem easily at present.Therefore searching is active by force from traditional medical material, active drug is hanged down in side effect becomes the new way that addresses this problem.Dai Nationality's medicine middle kidney tea is the doctor of Dai Nationality antidote kind commonly used; The Confucian classics record can singly be worked as tea-drinking with frying in shallow oil the immersion of soup or boiled water; Has heat-clearing and toxic substances removing, inducing diuresis to remove edema, diuresis fossil; Support kidney and protect kidney; Functions such as cooling blood for hemostasis, modern study show that chemical constituent mainly comprises flavonoid (Tezuka et al., 2000 in the kidney tea; Stampoulis et al.; 1999, Awale et al., 2001); Terpenoid (Tezuka et al.; 2000; Stampoulis et al., 1999, Awale et al.; 2001); Phenolic acids (Tezuka et al.; 2000, Akowuah et al., 2005); Amino acids (Zhang Ping; 2000); Vitamin; Mineral and volatile oil (Hossain et al., 2008).Pharmaceutical research shows that clerodendranthus spicatus extract has diuresis (Beaux et al., 1999; Yuliana et al., 2009, Adam et al.; 2009; Ting et al., 2009, Arafat et al.; 2008; Du Dat et al., 1992); Calculus (Cai Hua virtue et al., 2008); Improve chronic kidney hypofunction (Zhang Shaogui; 2008; Jiang Weisheng, 2009); Blood sugar lowering blood fat (Sriplang et al., 2007; Mohamed et al.; 2011); Antiinflammatory (high South-South et al., 1996, Yam et al.; 2010); Antioxidation (MATKOWSKI; 2008, Akowuah et al., 2004); Remove free radical (Akowuah et al.; 2005); Protect the liver (Mohammed; 2011, Maheswari et al., 2008); (Cen Xiaobo and Wang Rui is refined for human body immunity improving power; Pharmacologically active such as 1997); Wherein the hydrophilic active composition is phenolic acids (Li Yueting, 2003, Lin Zhimin; 2009); The lipotropy active component is a sinensetin; Eupatorin; And 3 '-hydroxyl-5,6,7; 4 '-tetramethoxy flavone (Akowuah et al.; 2004), the research and development to Herba clerodendranthi spicati and extract thereof have vast market prospect.
Summary of the invention
The objective of the invention is to from Herba clerodendranthi spicati
Clerodendranthus spicatus(Thunb.) extract a kind of extract among the C. Y. Wu ex H. W. Li.
Another object of the present invention is to provide the method for this extract of preparation.
Another purpose of the present invention is to provide pharmaceutical composition, comprises Herba clerodendranthi spicati
Clerodendranthus spicatus(Thunb.) carrier of using always in C. Y. Wu ex H. W. Li extract and the pharmaceutical field such as tablet, capsule, granule etc.
Another purpose of the present invention is to provide Herba clerodendranthi spicati
Clerodendranthus spicatus(Thunb.) C. Y. Wu ex H. W. Li extract and/or Herba clerodendranthi spicati
Clerodendranthus spicatus(Thunb.) application of compositions in treatment and prevent diabetes and complication medicine thereof of C. Y. Wu ex H. W. Li extract.
Another purpose of the present invention is to provide Herba clerodendranthi spicati
Clerodendranthus spicatus(Thunb.) C. Y. Wu ex H. W. Li extract and/or Herba clerodendranthi spicati
Clerodendranthus spicatus(Thunb.) application of compositions in treatment and prevent diabetes and complication health product thereof of C. Y. Wu ex H. W. Li extract.
Another purpose of the present invention is to provide Herba clerodendranthi spicati
Clerodendranthus spicatus(Thunb.) C. Y. Wu ex H. W. Li extract and/or Herba clerodendranthi spicati
Clerodendranthus spicatus(Thunb.) application of the compositions of C. Y. Wu ex H. W. Li extract in treatment and prevent diabetes associated metabolic class disease medicament.
Another purpose of the present invention is to provide Herba clerodendranthi spicati
Clerodendranthus spicatus(Thunb.) C. Y. Wu ex H. W. Li extract and/or Herba clerodendranthi spicati
Clerodendranthus spicatus(Thunb.) application of the compositions of C. Y. Wu ex H. W. Li extract in treatment and prevent diabetes associated metabolic class disease health product.
For accomplishing the object of the invention, the present invention implements following technical scheme:
Herba clerodendranthi spicati extract extraction process:
(1) getting Herba clerodendranthi spicati medical material behind the collection, dry in the shade, is to improve extraction ratio, and with pulverizing medicinal materials, granularity exceeds 200 mesh sieves most.
(2) get the medicinal powder that obtains in (1), the 10-30 that adds water and be quality of medicinal material doubly carries out reflux, extract, in 35-60 ℃, and extraction time is 1-3 time.
(3) get and extract the medicinal liquid that obtains in (2), in Rotary Evaporators, concentrate, the condensed water bath temperature is no more than 60 ℃, finally obtains the extractum that relative density is 1.15-1.5.
(4) get the extractum quality that obtains in (3) and be the resin of 20-50 times of quality and adorn post, add extractum, carry out eluting with the water of 20-40 times of resin column volume, the ethanol with the 50-80% of 20-40 times of resin column volume carries out eluting then, collects eluent.
(5) get the eluent of collecting in (4), in Rotary Evaporators, concentrate, the condensed water bath temperature is no more than 60 ℃, finally obtains the extractum that relative density is 1.15-1.5.
(6) get the extractum that obtains in (5), in vacuum drying oven, carry out drying under reduced pressure, heating-up temperature is no more than 60 ℃, finally obtains the Herba clerodendranthi spicati extract after the drying.The extract that obtains can carry out various preparations shapings.
The invention still further relates to and contain as the extract of the present invention of active component and the pharmaceutical composition of conventional medicine excipient or adjuvant.Usually pharmaceutical composition of the present invention contains the extract of the present invention of 0.1-95% weight.
The pharmaceutical composition of extract of the present invention can be according to the method preparation of this area public office.When being used for this purpose, if desired, extract of the present invention and one or more solids or liquid medicine excipient and/or adjuvant can be combined, process the suitable administration form or the dosage form that can be used as medicine or health product.
The specific embodiment
Through following examples and Test Example the present invention is explained further details, but this is not to concrete restriction of the present invention.
The relevant material of following examples, equipment etc. explain as follows:
Kidney tea (
Clerodendranthus spicatus(Thunb.) C. Y. Wu ex H. W. Li)
Embodiment 1 (preparation of clerodendranthus spicatus extract)
(1) get Herba clerodendranthi spicati medical material behind the collection, dry in the shade, for improving extraction ratio, with pulverizing medicinal materials, granularity 80 mesh sieves.
(2) get the medicinal powder that obtains in (1), add water and be 20 times of quality of medicinal material, carry out reflux, extract, in 45 ℃, extraction time is 2 times.
(3) get and extract the medicinal liquid that obtains in (2), in Rotary Evaporators, concentrate, the condensed water bath temperature is 50 ℃, finally obtains relative density and is about 1.28 extractum.
(4) get the extractum quality that obtains in (3) and be the resin of 30 times of quality and adorn post, add extractum, carry out eluting with the water of 40 times of resin column volumes, 80% ethanol with 40 times of resin column volumes carries out eluting, the collection eluent then.
(5) get the eluent of collecting in (4), in Rotary Evaporators, concentrate, the condensed water bath temperature is 50 ℃, finally obtains relative density and is about 1.28 extractum.
(6) get the extractum that obtains in (5), in vacuum drying oven, carry out drying under reduced pressure, heating-up temperature is 60 ℃, finally obtains the Herba clerodendranthi spicati extract after the drying.The extract that obtains can carry out various preparations shapings.
Embodiment 2 (Herba clerodendranthi spicati Stigma Maydis preparation of granules)
(1) get Herba clerodendranthi spicati medical material behind the collection, dry in the shade, for improving extraction ratio, with pulverizing medicinal materials, granularity 80 mesh sieves.
(2) get the medicinal powder that obtains in (1), add water and be 20 times of quality of medicinal material, carry out reflux, extract, in 45 ℃, extraction time is 2 times.
(3) get and extract the medicinal liquid that obtains in (2), in Rotary Evaporators, concentrate, the condensed water bath temperature is 50 ℃, finally obtains relative density and is about 1.28 extractum.
(4) get the extractum quality that obtains in (3) and be the resin of 30 times of quality and adorn post, add extractum, carry out eluting with the water of 40 times of resin column volumes, 80% ethanol with 40 times of resin column volumes carries out eluting, the collection eluent then.
(5) get the eluent of collecting in (4), in Rotary Evaporators, concentrate, the condensed water bath temperature is 50 ℃, finally obtains relative density and is about 1.28 extractum.
(6) get Stigma Maydis, grind, with deionized water heating and refluxing extraction 3 times, merge extractive liquid,, filtration.
(7) get (6) middle water extract filtrate decompression and be concentrated into 5 times, add 95% ethanol and make determining alcohol 70%-80%, standing over night, centrifugation removes albumen, and vacuum drying is subsequent use.
(8) get the extractum for preparing in 3 times of amounts (5) and the 1 times of amount (7), the lactose of 3 times of amounts of totalling extractum amount and the dextrin of 1 times of amount, the ethanol with 70% is made soft material, and squash type is granulated, and drying under reduced pressure, granulate finally obtain Herba clerodendranthi spicati Stigma Maydis granule.
Case study on implementation 3 (research of Herba clerodendranthi spicati extract function of blood sugar reduction)
1, extract preparation
Prepare according to method for preparing in the embodiment 1.
2, animal is irritated stomach
Get 40 of white mice, body weight 20 ± 2g, male and female half and half are separately fed, and behind the 24h, lumbar injection four pyrimidine 200mg/kg cause the experimental diabetes disease model, behind the 48h., are divided into four groups on an empty stomach, and 10 every group, male and female half and half, and irritate stomach, continuous irrigation stomach 5 days.It is following to irritate stomach dosage:
First group of blank group do not irritated stomach
Second group of positive controls irritated stomach insoral 150mg/kgd
The 3rd group of Herba clerodendranthi spicati extract low concentration group irritated stomach 100mg/kgd
The 4th group of Herba clerodendranthi spicati extract high concentration group irritated stomach 250mg/kgd
3, get blood
18h. behind the 5th filling stomach plucks eyeball by each six (every group each 1) and gets blood (0.3mL/ only), and in every test tube, adds anticoagulant trisodium citrate 0.1g in advance.Add 6mol/L sulphuric acid 5mL and 10% sodium tungstate 0.7mL after shaking up, shake up and handle 10min, the back gets supernatant and is serum with the centrifugal 15min of 3500r/min.
4, measuring blood content
Adopt the ortho-aminotoluene method to measure (Xu Shuyun, 1991).
5, result
Through relatively knowing that Herba clerodendranthi spicati extract high dose group can obviously reduce the white mice blood sugar concentration of the diabetes model that is caused by alloxan, specifically influence the result and see table 1.
Table 1 Herba clerodendranthi spicati extract is to the blood sugar influence result of experimental hyperglycemia white mice
Claims (9)
Priority Applications (1)
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103006781A (en) * | 2013-01-06 | 2013-04-03 | 张宁 | Compound Dai medicine extract with liver-protecting effect and preparation method thereof |
| CN103127214A (en) * | 2013-03-01 | 2013-06-05 | 中国医学科学院药用植物研究所云南分所 | Folium orthosiphoni effective part for treating diabetic nephropathy and preparation method |
| CN105902612A (en) * | 2016-05-27 | 2016-08-31 | 刘肖娣 | Preparation method and application of orthosiphon aristatus extract |
Citations (1)
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| CN101143160A (en) * | 2006-09-15 | 2008-03-19 | 李云森 | Kidney tea extract and its preparing process and application |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101143160A (en) * | 2006-09-15 | 2008-03-19 | 李云森 | Kidney tea extract and its preparing process and application |
Non-Patent Citations (1)
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| 李金雨: "猫须草的研究和开发利用进展", 《江西农业学报》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103006781A (en) * | 2013-01-06 | 2013-04-03 | 张宁 | Compound Dai medicine extract with liver-protecting effect and preparation method thereof |
| CN103006781B (en) * | 2013-01-06 | 2014-11-12 | 张宁 | Compound Dai medicine extract with liver-protecting effect and preparation method thereof |
| CN103127214A (en) * | 2013-03-01 | 2013-06-05 | 中国医学科学院药用植物研究所云南分所 | Folium orthosiphoni effective part for treating diabetic nephropathy and preparation method |
| CN105902612A (en) * | 2016-05-27 | 2016-08-31 | 刘肖娣 | Preparation method and application of orthosiphon aristatus extract |
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