CN104666643A - Compound plains coreopsis product, as well as preparation method and application thereof - Google Patents

Abstract

The invention relates to a compound snow chrysanthemum product and its preparation method and application. Calculated in parts by weight, the compound snow chrysanthemum product uses 0.8-6 parts by weight of snow chrysanthemum, 0.8-6 parts of Kuding tea, and 0.5-4 parts of vine tea 0.2-2 parts of stevia, and other edible or medicinal auxiliary materials are prepared into medicines, health care products or food according to the method of the present invention. When the compound snow chrysanthemum product of the present invention is applied to symptoms such as hypertension, hyperlipidemia and hyperglycemia, it has achieved good therapeutic effect, has the advantages of stability, high efficiency and safety, has simple preparation process, and is suitable for industrial production.

Description

A kind of compound snow chrysanthemum product and its preparation method and application

technical field

The invention belongs to the technical field of traditional Chinese medicine and health products, and in particular relates to a compound snow chrysanthemum product and its preparation method and application.

Background technique

"Three highs" refers to hypertension, hyperglycemia (diabetes) and hyperlipidemia. They are "diseases of wealth" derived from modern society, and they may exist independently or be related to each other. For example, diabetics are prone to suffer from high blood pressure or hyperlipidemia at the same time, and hyperlipidemia is the main factor in the formation and development of arteriosclerosis. The poor elasticity of blood vessels in patients with arteriosclerosis aggravates the increase in blood pressure. Therefore, any one of these three diseases will easily form the "three highs" in the later stage. With the improvement of living standards and changes in the rhythm of life, the "three highs" (i.e. high blood pressure, high blood sugar and high blood fat), known as "rich man's disease", has become a common disease in the health of middle-aged and elderly people over 50 years old. The number of people who die from cardiovascular and cerebrovascular diseases is as high as 15 million every year in the world, ranking first among various causes of death. Among the top ten causes of death in China, the mortality rate related to metabolic diseases is as high as 35.7%, and the number of deaths related to "three highs" also accounts for 27% of the total number of deaths. The World Health Organization once clearly stated that the first line of defense to prevent cardiovascular disease is to reduce and control the "three highs".

Chrysanthemum is the dry flower head of Chrysanthemum morifolium Ramat., a plant of Compositae. It is sweet, bitter, and slightly cold. There are many kinds of chrysanthemums, and different chrysanthemums have certain differences in medicinal effects, such as Chuju clearing heat and detoxifying, relaxing tendons and promoting blood circulation, protecting liver and improving eyesight, white chrysanthemum clearing liver and improving eyesight, yellow chrysanthemum dispelling wind and cooling, wild chrysanthemum clearing heat and detoxifying, stevia rebaudiana Regulating blood pressure, lowering fat, lowering blood sugar, snow chrysanthemum detoxification and dampness, etc. Therefore, only by choosing the right type of chrysanthemum, matching with reasonable medicinal materials, and aiming at specific health care purposes, can we achieve twice the result with half the effort.

In the prior art, there are many reports on the use of chrysanthemum as a health product, and there are many related documents on the prevention and treatment of hypertension, hyperlipidemia or hyperglycemia. For example, patent CN201110451691.3 discloses a compound health care product containing rattan tea. The tea and its preparation method are prepared from the leaves and stems of vine tea together with chrysanthemum, mulberry and lotus leaves. However, this invention mainly highlights the effects of the young leaves and stems of vine tea, and the amount of chrysanthemum is very small. And it does not specify what kind of chrysanthemum is the best medicinal ingredient; patent application CN201410628445.4 discloses a Kudingcha wine and its preparation method, using the effect of wine to promote blood circulation and remove blood stasis, with Kudingcha, kudzu root slices, hawthorn, Stevia powder, rushes, ebony and other medicinal materials can achieve the purpose of detoxifying, removing blood stasis, promoting blood circulation and lowering blood pressure. The health wine of this invention provides consumers with another choice of wine products; patent application CN201310658985.2 discloses the method of lowering blood pressure Traditional Chinese medicine composition and its preparation method. The composition is made of 12 kinds of medicinal materials such as burdock, apocynum, snow chrysanthemum, kudingcha, corn silk, and mulberry. failed to achieve the desired effect.

Although there are many Chinese and Western medicines and health care products for hypertension, hyperlipidemia and hyperglycemia, there are few reports that are effective for the "three highs" at the same time. few. The researchers of the present invention used snow chrysanthemum as the basic raw material for eliminating the three highs, and carried out careful and careful screening and research on medicinal materials shared with other types of chrysanthemums, tea leaves, and some medicines and foods, in order to bring blood pressure and blood lipids to the majority of patients. It is a product that is safe and efficient in terms of blood sugar and hypoglycemia, and has a variety of dosage forms, which can be used by different groups of people. So far, there are no related reports on the composition or compound preparation made of snow chrysanthemum, rattan tea, kudingcha and stevia and its simultaneous reduction of "three highs".

Contents of the invention

The purpose of the present invention is to provide a compound snow chrysanthemum product with simple composition, high utilization rate of active ingredients, and various product types and its preparation method, as well as the preparation of medicines and health care products with three functions of lowering blood pressure, blood fat and blood sugar or food applications.

The present invention is achieved like this:

A compound snow chrysanthemum product, characterized in that the product is made of the following raw materials in parts by weight: 0.8-6 parts of snow chrysanthemum, 0.8-6 parts of Kuding tea, 0.5-4 parts of vine tea, 0.2-2 parts of stevia share.

Further, the weight parts of the raw materials of the product are: 1.5-3 parts of snow chrysanthemum, 1.5-3 parts of Kudingcha, 1-2 parts of rattan tea, and 0.5-1 part of Stevia.

The aforementioned compound snow chrysanthemum product can be medicine, health product or food.

Further, the dosage form of the medicine or health care product is tablet, capsule, granule or oral liquid; the type of the food is tea beverage, tea in bag.

The invention also discloses a preparation method of the compound snow chrysanthemum product.

Wherein, the preparation method of medicine or health product comprises the following steps:

1) Pulverize the snow chrysanthemum, extract the volatile oil by steam distillation for later use; use 6-10 times the amount of ethanol with a concentration of 65%-100% to reflux extract the dregs and stevia leaves for 1-3 times, each time for 0.5-1.5 hours , filter the extract, combine the filtrates, and concentrate to make an extract with a relative density of 1.15-1.30 at 55-60°C;

2) Take vine tea and Kuding tea, decoct and extract them with 6-10 times the amount of water for 2-4 times, each time for 1-3 hours, filter the extracts, combine the filtrates, concentrate and make them relative to each other at 55-60°C An extract with a density of 1.10-1.30;

3) Spray-dry the extracts of the above-mentioned snow chrysanthemum, stevia, vine tea and kudingcha, or directly add the extracted snow chrysanthemum volatile oil, add pharmaceutical excipients, and make the required tablets, Capsules, granules, oral liquid.

Specifically, tablets are prepared as follows:

1) Pulverize the snow chrysanthemum, extract the volatile oil by steam distillation for later use; use 6-10 times the concentration of 20%-50% ethanol to reflux extract the dregs and stevia leaves for 1-3 times, each time for 0.5-1.5 hours , filter the extract, combine the filtrates, and concentrate to make an extract with a relative density of 1.15-1.30 at 55-60°C;

2) Take vine tea and Kuding tea, decoct and extract them with 6-10 times the amount of water for 2-4 times, each time for 1-3 hours, filter the extracts, combine the filtrates, concentrate and make them relative to each other at 55-60°C An extract with a density of 1.10-1.30;

3) Spray-dry the extract extracts of snow chrysanthemum, stevia rebaudiana, rattan tea and kudingcha prepared above, and add microcrystalline cellulose of 15-20% of the total amount of the tablet in the dried medicated powder, The rest is replenished to the full amount with pregelatinized starch, added the collected chrysanthemum volatile oil, mixed evenly, granulated with 8% starch slurry, dried, added 0.5-1% magnesium stearate of the total amount of the tablet, and compressed into tablets, that is have to.

Capsules are prepared like this:

1) Pulverize the snow chrysanthemum, extract the volatile oil by steam distillation for later use; use 6-10 times the concentration of 20%-50% ethanol to reflux extract the dregs and stevia leaves for 1-3 times, each time for 0.5-1.5 hours , filter the extract, combine the filtrates, and concentrate to make an extract with a relative density of 1.15-1.30 at 55-60°C;

2) Take vine tea and Kuding tea, decoct and extract them with 6-10 times the amount of water for 2-4 times, each time for 1-3 hours, filter the extracts, combine the filtrates, concentrate and make them relative to each other at 55-60°C An extract with a density of 1.10-1.30;

3) Spray-dry the extract extracts of snow chrysanthemum, stevia rebaudiana, rattan tea and kudingcha prepared above, add microcrystalline cellulose of capsule total amount 12-15% in the dried medicine powder, The rest is supplemented to the full amount with cornstarch, the collected chrysanthemum volatile oil is added, mixed uniformly, granulated, dried, added with 0.5-1% magnesium stearate in the total amount, mixed evenly and packed into capsules to obtain the product.

Granules are prepared like this:

1) Pulverize the snow chrysanthemum, extract the volatile oil by steam distillation for later use; use 6-10 times the concentration of 20%-50% ethanol to reflux extract the dregs and stevia leaves for 1-3 times, each time for 0.5-1.5 hours , filter the extract, combine the filtrates, and concentrate to make an extract with a relative density of 1.15-1.30 at 55-60°C;

2) Take vine tea and Kuding tea, decoct and extract them with 6-10 times the amount of water for 2-4 times, each time for 1-3 hours, filter the extracts, combine the filtrates, concentrate and make them relative to each other at 55-60°C An extract with a density of 1.10-1.30;

3) Spray-dry the extracts of the above-mentioned snow chrysanthemum, stevia, rattan tea and Kudingcha, add the collected snow chrysanthemum volatile oil and dextrin into the dried medicinal powder, mix well, and prepare Granules, dried, granulated, packaged to obtain granules.

The oral solution is prepared like this:

1) Pulverize the snow chrysanthemum, extract the volatile oil by steam distillation for later use; use 6-10 times the concentration of 20%-50% ethanol to reflux extract the dregs and stevia leaves for 1-3 times, each time for 0.5-1.5 hours , filter the extract, combine the filtrates, and concentrate to make an extract with a relative density of 1.15-1.30 at 55-60°C;

2) Take vine tea and Kuding tea, decoct and extract them with 6-10 times the amount of water for 2-4 times, each time for 1-3 hours, filter the extracts, combine the filtrates, concentrate and make them relative to each other at 55-60°C An extract with a density of 1.10-1.30;

3) Add water to the extracts of snow chrysanthemum, stevia, vine tea and kudingcha prepared above to 50% of the total amount, add the collected snow chrysanthemum volatile oil, mix well, add simple syrup to the sugar content 15-20%, adding 0.1% sodium benzoate, adding distilled water to the full amount, mixing, filtering, potting to obtain oral liquid.

Wherein, the preparation method of compound snow chrysanthemum tea beverage comprises the following steps:

1) Pulverize the leaves of snow chrysanthemum and stevia rebaudiana, reflux extract with 6-10 times the amount of ethanol with a concentration of 20%-50% for 1-3 times, each time for 0.5-1.5 hours, filter the extract, combine the filtrates, and set aside;

2) Take vine tea and Kuding tea, decoct and extract 2-4 times with 6-10 times the amount of water, each time for 1-3 hours, filter the extract, combine the filtrate, and set aside;

3) Add chitosan with 1% crude drug amount to the extract solution prepared in steps 1 and 2, let it stand for 24 hours, filter, add water to the filtrate, stir well, adjust the pH value to 4-5, add the total amount of beverage 6-9% of rock sugar and 0.05-0.12% of citric acid are sterile filtered, the filtrate is subpackaged, capped, and made into a beverage.

Wherein the preparation method of compound snow chrysanthemum teabag comprises the following steps:

1) Take the snow chrysanthemum and stevia in the formula amount, vacuum-dry at 0-6°C until the water content is less than 5%, pulverize into fine powder, and pass through a 60-mesh sieve for later use;

2) Take 50%-70% of the formula amount of Kudingcha and grind it into a coarse powder with a particle size of 0.2-0.3mm, add 6-10 times the amount of the remaining Kudingcha and the formula amount of rattan tea branches and leaves, and the concentration is 55%- Reflux extraction with 75% ethanol solution for 1-2 times, each time for 1-2 hours, combine the extracts, filter, and concentrate to make an extract with a relative density of 1.20-1.30 at 55-60°C;

3) Turn on the frying pot, adjust the temperature to 75-85°C, and the speed of the stirring rod to 80-120 rpm, put in the coarse powder of Kudingcha and stir-fry for 10-20 minutes, then add the snow inulin powder and Stevia rebaudiana powder, and the extract extract prepared in step 2), after fully mixing, put the mixture into a sterilizing and drying equipment, first sterilize at high temperature, then quickly cool down to below 80°C, dry at low temperature, pass through 80 mesh Sieve the whole grain, pack it separately, and get it.

Another object of the present invention is to provide the application of the aforementioned compound snow chrysanthemum product in the preparation of products with three functions of lowering blood pressure, blood sugar and blood fat.

Compared with prior art, the raw material that the present invention adopts, effect and mechanism are as follows:

Snow chrysanthemum: Also known as two-color coreopsis and Kunlun snow chrysanthemum, it is mild in nature and sweet in taste. It has the functions of clearing away heat, detoxification and dehumidification. Native to the midwestern region of the United States and cultivated in western China. Due to the low pollution of the growth environment and high altitude, it contains 18 kinds of amino acids, more than 30 kinds of flavonoids and mineral elements necessary for the human body, as well as rich organic acids and terpenes. Alkenes, vitamins, enzymes and polysaccharides have special curative effects on hypertension, hyperlipidemia, hyperglycemia, coronary heart disease, etc., can significantly reduce serum triglycerides and cholesterol, and have sterilization, anti-inflammatory, weight loss, prevention of colds and chronic The efficacy of enteritis also has a very good conditioning effect on insomnia. Among them, the total flavonoid content reaches 12%, far exceeding other various chrysanthemums.

Stevia: It is a perennial herb of Compositae. The leaves contain inulin. Its sweetness is 150-300 times that of sucrose. It is an excellent natural sweetener. Moreover, it can treat certain diseases, such as diabetes and blood pressure lowering. It also has curative effects on obesity, heart disease, and children's teeth. It can also promote metabolism and strengthen the body. The leaves contain stevioside (Stevioside), steviolbioside (Steviolbioside), stevioside A, B (rebaudisides A, B) and other active ingredients and sweet ingredients. Pharmacological tests show that its active ingredient stevioside inhibits the synthesis of sugar and oxygen absorption, and the blood sugar level drops significantly; in addition, it also has obvious vasodilator effect, so it has a significant effect on regulating blood pressure, softening blood vessels, lowering blood fat, blood sugar, and urine sugar. It also has the effects of antibacterial and hemostasis, analgesia, weight loss and beautification, nourishing yin and promoting body fluid, helping digestion, promoting pancreas, spleen and stomach functions, and clearing away heat and detoxification.

Vine tea: also known as Maoyan berry tea, dragon beard tea, longevity vine, etc., and its scientific name is tooth snake grape. It is rich in 17 kinds of amino acids and 14 kinds of trace elements such as potassium, calcium and iron. Among them, the active ingredients dihydromyricetin, anthocyanin and flavonoids are high in content. It has the functions of lowering blood sugar, moistening throat and relieving cough, and improving human immunity. In terms of the health effects of vine tea, its three lowering (lowering blood viscosity, lowering blood pressure, lowering blood sugar), third anti (antithrombotic, anti-aging, anti-tumor), and three regulating (regulating blood lipid, regulating immunity, regulating gastrointestinal) effects are significant. .

Kudingcha: Kudingcha is an evergreen tree belonging to the holly family Ilex, commonly known as Chading, Fudingcha, and Gaolucha. It is mainly distributed in the southwest region. It is sweet, bitter, and cold in nature. It is a traditional natural tea in my country. Excellent health drink. Kuding tea contains more than 200 kinds of ingredients such as Kuding saponin, amino acid, vitamin C, polyphenols, flavonoids, caffeine, protein, etc., which can reduce blood fat, increase coronary blood flow, increase myocardial blood supply, and resist atherosclerosis. It has a good preventive effect on dizziness, headache, chest tightness, fatigue, insomnia and other symptoms in patients with cardiovascular and cerebrovascular diseases. The finished tea has a bitter taste and a sweet and cool taste. It has many functions such as clearing away heat and relieving heat, improving eyesight and improving intelligence, promoting body fluid and quenching thirst, diuretic and strengthening heart, moistening throat and relieving cough, lowering blood pressure and losing weight, suppressing cancer and preventing cancer, anti-aging, and promoting blood circulation. .

To sum up, the present invention utilizes snow chrysanthemum to detoxify and remove dampness, and significantly reduce serum triglyceride and cholesterol. Combined with stevia to soften blood vessels, reduce fat and blood sugar, and the health effects of vine tea with three reductions, three resistances, and three conditions, As well as Kudingcha for lowering blood pressure, strengthening heart, anti-aging and promoting blood circulation, the active ingredients are synergistic and synergistic, which can effectively prevent and treat hypertension, hyperlipidemia, hyperglycemia and related metabolic diseases.

Adopt test to further illustrate the present invention below:

Experiment 1, "Three Drops" Effect Test of the Product of the Present Invention - Formula Screening

1. Test sample

Test samples: samples of the present invention: tablets, capsules and granules made by selecting examples 1, 3, 4, 10, and 11, numbered samples 1-5;

Control sample 1: the extract prepared according to Example 1 of patent application CN201310658985.2 was concentrated to make an extract with a relative density of 1.20 at 60°C;

Control sample 2: according to the tablet prepared by the formula and preparation method of Example 1, which does not contain Stevia;

Control sample 3: according to the tablet prepared by the formula and the preparation method of Example 1, wherein the vine tea is replaced by kudzu root;

Control sample 4: a tablet prepared according to the formula and method of Example 1, wherein Kudingcha was replaced with Keemun black tea.

2. Test method

2.1 Pharmacological research on blood lipid lowering

2.1.1 Test animals

SD rats, weighing 180-220g, half male and half female, clean standard, were randomly divided into 12 groups: blank control group, model group, Di'ao Xinxuekang group, sample 1-5 group, control sample 1-4 group, 10 rats in each group, Raise in separate cages. Except for the blank control group, after the rats in the other groups were continuously given high-fat diet for 14 days, 30% of the animals in each group were randomly selected to take blood from the vascular plexus behind the eyeball, and the blood lipids such as total cholesterol (TC) and triglyceride (TG) were detected. level. As a result, compared with the blank control group, the success rate of the hyperlipidemia model reached 99%, which proved that the model was successfully constructed. After excluding individual animals with low blood lipid levels, the lipid-lowering drug experiment was carried out.

2.1.3 Test method

After modeling, the model group and the blank control group were fed normally, and ^1ml of normal saline was given to the stomach every day. tablets, administered 0.15g·kg ^-1 ; sample group 2 was capsules, administered 0.1g·kg ^-1 ; sample group 3 was granules, administered 0.8g·kg ^-1 ; comparative sample group 1 was infused Ointment, administration is equivalent to 7g of crude drug·kg ^-1 ; equivalent to 10 times of clinical dosage. The above samples were grinded, mixed evenly before administration, and dissolved in distilled water to make each 1ml containing the above dose.

The administration began on the 15th day of the high-fat feed feeding, and each animal group in the experiment was administered orally by gavage, 1 ml once a day, for a total of four weeks and 28 days (after the last administration, fasting for 8 hours). Blood was collected from all animals through the orbit, and serum was collected to detect blood lipid levels after administration.

2.2 Hypoglycemic pharmacology research

2.2.1 Test animals: NIH mice, male, weighing 18.0-23.1g, clean standard. Normal mice were injected with alloxan (72mg·kg ^-1 ) through the tail vein, and the serum glucose level was predicted 72 hours later, and the blood glucose level above 16mmol·L ^-1 was selected for research. They were randomly divided into 12 groups: blank control group, model group, sample 1-5 group, control sample 1-4 group, metformin hydrochloride (150 mg·kg ^-1 ) group, 10 rats in each group, and raised in separate cages.

2.2.2 Test method

After modeling, the model group and the blank control group were fed normally, and 1ml of normal saline was administered to the stomach every day. kg ^-1 ; sample group 2 is capsules, administered at 0.1g·kg ^-1 ; sample group 3 is granules, administered at 0.8g·kg ^-1 ; comparative sample group 1 is extract, equivalent to 7g crude drug·kg ^-1 ; equivalent to 10 times of clinical dosage. The above samples were grinded and mixed evenly before administration, and dissolved in distilled water to make each 1ml containing the above dose, 1ml once a day, and administered continuously for 7 days. One hour after the last administration, blood was collected from the orbital venous plexus of the mice to measure the blood glucose level.

2.3 Antihypertensive pharmacological research

2.3.1 Test animals: Wistar rats, male, weighing 250±26.3g, were randomly divided into 12 groups: control group (potassium chloride), sample 1-5 group, control sample 1-4 group, propranolol group, 10 rats in each group were housed in separate cages.

2.3.2 Test method: thoracic artery isolated vessel tension study method

2.3.2.1 Preparation and stabilization of thoracic aortic ring:

Rats were decapitated, and the thoracic aorta was quickly taken out by opening the chest, placed in KH solution at 4°C saturated with mixed gas (95% O ₂ and 5% CO ₂ ), washed until there was no blood, and the peripheral blood was carefully removed. Adipose tissue and connective tissue were cut into 2-3mm long vascular rings, and the vascular rings were placed in 10ml of KH solution (at a constant temperature of 37°C, and a mixed gas of 95% O ₂ and 5% CO ₂ was continuously introduced, In the constant temperature jacket bottle with pH=7.4), each blood vessel ring is penetrated by two triangular hooks through the lumen of the blood vessel ring, one end is fixed, and the other end is connected to the tension sensor, and the tension change is collected and recorded by the BL-420E biological function experiment system . The vascular ring was added with a resting tension of about 2g, and it was stable for 90 minutes. During this period, the liquid was replaced with KH solution pre-warmed to 37°C every 15 minutes. After the vascular ring is completely stable, the formal experiment can be carried out.

2.3.2.2 Effect of KCl precontracted vascular ring tension

After the vascular ring was stabilized, it was repeatedly stimulated with 60mmol/L KCl 3 times to induce the maximum contraction range of the blood vessel. When the contraction range difference caused by 3 consecutive same stimulations was less than 10%, it was regarded as 100% contraction tension. Add the test drugs respectively according to the dosage in Table 3.

3. Results

3.1 Results of blood lipid lowering test

According to the statistics, the blood lipid lowering effect is shown in Table 1 below.

Table 1 The blood lipid-lowering effect of compound snow chrysanthemum products

*P<0.05 compared with the comparison sample group, Compared with the model group, P<0.05.

After a course of treatment, both the sample group and the control sample group reduced the levels of total cholesterol (TC) and triglyceride (TG) at different levels. But it can be clearly seen that the effect of the sample 1-3 of the present invention is the best in tablets, capsules and granules, which is significantly higher than the therapeutic effect of the Di'ao Xinxuekang group, indicating that the curative effect of the compound snow chrysanthemum product of the present invention is affirmative; compared with comparative samples 1-4 (i.e. lack of one medicinal material, or change the medicinal material collocation), show that the prescription medicinal material combination of the present invention is adopted, and the formula is reasonable; 4 groups of samples with low formula quantity have unsatisfactory treatment effects, and the curative effect of 5 groups of samples with high formula quantity is the same as that of sample 1- There is no difference compared with 3 groups, the consumption, safety and economy etc. of comprehensive medical material, therefore select the formula consumption range of the present invention.

3.2 The results of hypoglycemic test

The statistics are shown in the table below.

Table 2 Hypoglycemic effect of compound snow chrysanthemum products

*P<0.05 compared with the comparison sample group, Compared with the model group, P<0.05.

After 7 days of treatment, the sample group and the control sample group all reduced the blood sugar level of the model mice in varying degrees, but it can be clearly seen that the sample 1-3 of the present invention is the best effect of tablets, capsules and granules, which is obviously higher than The therapeutic effect of metformin hydrochloride group shows that the curative effect of compound snow chrysanthemum product of the present invention is affirmative; Compared with comparative samples 1-4 (that is, lacking a single medicinal material, or changing medicinal material collocation), it is shown that the prescription medicinal material combination of the present invention is adopted, and the formula is reasonable; low formula 4 groups of samples made by the amount of treatment effect are not ideal, 5 groups of samples with high formula quantity have no difference in curative effect compared with sample 1-3 groups, and the consumption, safety and economy of comprehensive medicinal materials etc., therefore select the formula consumption of the present invention scope.

3.3 Results of blood pressure lowering test

According to statistics, the effect of lowering blood pressure is shown in Table 3 below.

Table 3 Antihypertensive effect of compound snow chrysanthemum products

The above results show that the sample group and the control sample group all reduce the blood pressure level of the model mice in varying degrees, but it can be clearly seen that the sample 1-3 of the present invention is the best effect of tablets, capsules and granules, which is higher than that of propranol. The treatment effect of Er group shows that the curative effect of the compound snow chrysanthemum product of the present invention is affirmative; Compared with comparative samples 1-4 (that is, lacking a single medicinal material, or changing medicinal material collocation), it is shown that the prescription medicinal material combination of the present invention is adopted, and the formula is reasonable; low formula quantity The curative effect of 4 groups of samples made is not ideal, the curative effect of 5 groups of samples with high formula quantity is no difference compared with sample 1-3 groups, the consumption, safety and economy etc. of comprehensive medicinal material, therefore select the formula dosage range of the present invention .

Experiment 2, preparation process research

1. Preparation of medicines or health products:

The invention firstly collects the volatile oil of snow chrysanthemum, which avoids the disadvantage of using only the decocted medicinal liquid in the traditional method; adopts the method of low-concentration ethanol reflux to extract active ingredients such as total flavonoids and steviosides in snow chrysanthemum and stevia The method of decocting in water extracts dihydromyricetin, total flavonoids, total saponins and amino acids in vine tea and Kudingcha, which not only fully extracts the active ingredients, but also reduces the difference between the ingredients by separating them. The mutual influence of active ingredients improves the purity of active ingredients and reduces the introduction and content of impurities; the extract paste is dried by spray drying, which has low energy consumption, low pollution, rapid water evaporation, short drying time, and eliminates the need for evaporation and crushing. And other processes, and the product quality is good, the stability is strong, the solubility is high.

2. Preparation of teabag:

In the present invention, the snow chrysanthemum and stevia are vacuum-dried at low temperature, which effectively maintains the efficacy of the active ingredients of the medicine, as well as the original fragrance and taste of chrysanthemum; Fragrance and glycol, and because the remaining part and vine tea are extracted with water to obtain the extract, the active ingredients are fully utilized, and the dissolution of the teabag is also improved. In this way, the obtained teabags have achieved very good results in terms of color, fragrance, taste, and efficacy.

Experiment 3, the safety test of product of the present invention

Take the samples A1-A9 prepared in Examples 1-9 of the present invention, according to the dosage of 10 times, 20 times, and 50 times of the human dosage, carry out toxicological experiments with mice. The results of animal experiments show that the human dosage is 10 times, 20 times There is no significant change in body weight, lack of energy, decreased activity or other symptoms in mice when the dosage is doubled, and there is a slight listlessness phenomenon when the dosage is 50 times that of humans. It is proved that the product of the invention has little toxic and side effects and is very beneficial to clinical use.

Experiment 4, the stability test of product of the present invention

Get the samples A1-A9 that the embodiment of the present invention 1-9 makes, carry out accelerated stability test under the condition of temperature 40 ℃ ± 2 ℃, relative humidity 75% ± 5%. Sampling at the end of each month, 3 months, and 6 months for inspection; the stability of the product is checked using the properties, moisture, content of effective active ingredients, and hygienic inspection as indicators. The results show that: the product prepared by the present invention is stored under accelerated test conditions, and there is no abnormality in each investigation item, the moisture change is controlled within 5%, the active ingredient content does not change significantly, and its hygienic inspections all meet the expected requirements, indicating The product quality of the invention meets the requirements.

From the above data, it can be seen that the products made of snow chrysanthemum, stevia, rattan tea, kudingcha and other ingredients in the present invention can effectively prevent the "three high" diseases. All well meet the expected requirements, indicating that the formula design of the present invention is reasonable, the preparation method is simple and controllable, and has good application prospects.

Detailed ways

The present invention is further explained below in conjunction with examples and test examples, but the content of the present invention is not limited to the following description.

Example

Embodiment 1: tablet and preparation

Recipe: Snow Chrysanthemum 2.5kg, Kudingcha 2.0kg, Rattan Tea 1.5kg, Stevia 1kg

Preparation method: 1) Pulverize snow chrysanthemum, extract volatile oil by steam distillation for later use; use 8 times the concentration of 30% ethanol to reflux extract the dregs and stevia leaf for 2 times, each time for 1 hour, filter the extract, and filter the filtrate Merge and concentrate to make an extract with a relative density of 1.20 at 55-60°C;

2) Take vine tea and Kuding tea, decoct and extract them with 8 times the amount of water for 3 times, 2 hours each time, filter the extract, combine the filtrates, concentrate to make an extract with a relative density of 1.20 at 55-60°C ;

3) Spray-dry the extract extracts of the above-mentioned snow chrysanthemum, stevia, vine tea and Kudingcha, add 15% microcrystalline cellulose of the total amount of the tablet in the dried medicated powder, and use the rest with Replenish the pregelatinized starch to the full amount, add the collected chrysanthemum volatile oil, mix evenly, granulate with 8% starch slurry, dry, add 1% magnesium stearate of the total amount of the tablet, and compress into tablets to obtain 1000 tablets piece. Each piece weighs 0.5g.

Usage and dosage: 2 tablets at a time, 2-3 times a day.

Embodiment 2: tablet and preparation

Recipe: Snow Chrysanthemum 2.0kg, Kudingcha 2.5kg, Rattan Tea 0.5kg, Stevia 0.5kg

Preparation method: 1) Pulverize snow chrysanthemum, extract volatile oil by steam distillation for later use; use 8 times the amount of 40% ethanol to reflux extract the dregs and stevia leaf for 2 times, each time for 1 hour, filter the extract, and filter the filtrate Merge and concentrate to make an extract with a relative density of 1.15 at 60°C;

2) Take rattan tea and Kuding tea, decoct and extract 3 times with 8 times the amount of water, 2 hours each time, filter the extract, combine the filtrates, concentrate and make an extract with a relative density of 1.10 at 60°C;

3) Spray-dry the extract extracts of the aforementioned snow chrysanthemum, stevia rebaudiana, vine tea and Kudingcha, add 20% microcrystalline cellulose of the total amount of the tablet to the dried medicated powder, and use the remaining Replenish the pregelatinized starch to the full amount, add the collected chrysanthemum volatile oil, mix evenly, granulate with 8% starch slurry, dry, add 0.5% magnesium stearate in the total amount of the tablet, and compress the tablet to obtain 1000 tablets piece.

Embodiment 3: capsule and preparation

Recipe: Snow Chrysanthemum 0.8kg, Kudingcha 0.8kg, Rattan Tea 0.5kg, Stevia 0.2kg

Preparation method: 1) Pulverize the snow chrysanthemum, extract the volatile oil by steam distillation for later use; use 10 times the amount of 20% ethanol to reflux extract the dregs and stevia leaves for 3 times, each time for 0.5 hours, filter the extract, and filter the filtrate Combined and concentrated to make an extract with a relative density of 1.30 at 55°C;

2) Take rattan tea and Kuding tea, decoct and extract them twice with 10 times the amount of water, each time for 3 hours, filter the extract, combine the filtrates, and concentrate to make an extract with a relative density of 1.30 at 55°C;

3) Spray-dry the extract extracts of the above-mentioned snow chrysanthemum, stevia, vine tea and Kudingcha, add 12% microcrystalline cellulose of the capsule total amount in the dried medicated powder, and use all the other The cornstarch is supplemented to the full amount, the collected chrysanthemum volatile oil is added, mixed uniformly, granulated, dried, and 0.5-1% magnesium stearate is added in a total amount, mixed evenly and packed into capsules to obtain 1000 capsules. Each capsule contains 0.3g.

Usage and dosage: 2 capsules each time, 2-3 times a day.

Embodiment 4: Granule and preparation

Recipe: Snow Chrysanthemum 6kg, Kudingcha 6kg, Rattan Tea 4kg, Stevia 2kg

Preparation method: 1) Pulverize snow chrysanthemum, extract volatile oil by steam distillation for later use; use 6 times the amount of 50% ethanol to reflux extract for 1.5 hours, filter the extract, concentrate and prepare at 60°C The extract with a relative density of 1.25;

2) Take rattan tea and Kuding tea, decoct and extract 4 times with 6 times the amount of water, each time for 1 hour, filter the extract, combine the filtrates, concentrate to make an extract with a relative density of 1.20 at 60°C;

3) Spray-dry the extracts of the above-mentioned snow chrysanthemum, stevia, rattan tea and Kuding tea, add the collected snow chrysanthemum volatile oil and dextrin to the dried medicinal powder to supplement the whole amount, mix homogeneously, granulated, dried, sized and packaged to obtain 1000 bags of granules. 5g per bag.

Usage and dosage: 1 bag at a time, 2-3 times a day.

Embodiment 5: oral liquid and preparation

Recipe: Snow Chrysanthemum 1.5kg, Kudingcha 1.5kg, Rattan Tea 1kg, Stevia 0.5kg

Preparation method: 1) Pulverize the snow chrysanthemum, extract the volatile oil by steam distillation for later use; use 9 times the amount of 25% ethanol to reflux extract the dregs and stevia leaf for 2 times, each time for 1.5 hours, filter the extract, and filter the filtrate Merge and concentrate to make an extract with a relative density of 1.15 at 60°C;

2) Take rattan tea and Kuding tea, decoct and extract 4 times with 9 times the amount of water, each time for 1 hour, filter the extract, combine the filtrates, concentrate to make an extract with a relative density of 1.25 at 60°C;

3) Add water to the extracts of snow chrysanthemum, stevia, vine tea and kudingcha prepared above to 50% of the total amount, add the collected snow chrysanthemum volatile oil, mix well, add simple syrup to the sugar content 15%, add 0.1% sodium benzoate, add distilled water to the full amount, mix evenly, filter, potting, and obtain 1000 oral liquids. 10ml each.

Usage and dosage: 10ml each time, 2-3 times a day.

Embodiment 6: tea beverage and preparation

Recipe: Snow Chrysanthemum 4kg, Kudingcha 1.5kg, Rattan Tea 1kg, Stevia 0.5kg

Preparation method: 1) Pulverize the leaves of snow chrysanthemum and stevia rebaudiana, reflux extract with 6 times the amount of 40% ethanol for 3 times, each time for 0.5 hours, filter the extract, combine the filtrate, and set aside;

2) Take rattan tea and Kuding tea, decoct and extract them twice with 10 times the amount of water, each time for 2 hours, filter the extracts, combine the filtrates, and set aside;

3) Add chitosan with 1% crude drug amount to the extract solution prepared in steps 1 and 2, let it stand for 24 hours, filter, add water to the filtrate, stir well, adjust the pH value to 4-5, add the total amount of beverage 6% rock sugar, 0.05% citric acid, sterile filtration, sub-package of the filtrate, capping, and make 1000 boxes of beverages. 150ml per box.

Usage and dosage: 1 bottle at a time, 2-3 times a day.

Embodiment 7: tea beverage and preparation

Recipe: Snow Chrysanthemum 3kg, Kudingcha 3kg, Rattan Tea 2kg, Stevia 1kg

Preparation method: 1) crush the leaves of snow chrysanthemum and stevia rebaudiana, reflux extract with 10 times the concentration of 30% ethanol for 1.5 hours, filter the extract, and set aside;

2) Take rattan tea and Kuding tea, decoct and extract them with 6 times the amount of water for 4 times, each time for 1 hour, filter the extracts, combine the filtrates, and set aside;

3) Add chitosan with 1% crude drug amount to the extract solution prepared in steps 1 and 2, let it stand for 24 hours, filter, add water to the filtrate, stir well, adjust the pH value to 4-5, add the total amount of beverage 9% rock sugar, 0.12% citric acid, aseptically filtered, the filtrate is subpackaged, capped, and made into 1000 boxes of beverages. 150ml per box.

Embodiment 8: tea bag and preparation

Recipe: Snow Chrysanthemum 6kg, Kudingcha 3kg, Rattan Tea 4kg, Stevia 2kg

Preparation method: 1) Take snow chrysanthemum and stevia rebaudiana in the formula amount, vacuum-dry at 0°C until the water content is less than 5%, pulverize into fine powder, pass through a 60-mesh sieve for later use;

2) Take Kudingcha with 50% of the recipe and crush it into a coarse powder with a particle size of 0.2-0.3 mm. Add 6 times the amount of the remaining Kudingcha and the branches and leaves of Rattan tea with the recipe to reflux and extract 2 times with 85% ethanol solution , 1 hour each time, combine the extracts, filter, and concentrate to make an extract with a relative density of 1.20-1.30 at 55-60°C;

3) Turn on the frying pot, adjust the temperature to 75°C, and the speed of the stirring rod to 80 rpm, put in the coarse powder of Kudingcha and stir-fry for 10 minutes, then add the snow chrysanthemum powder and stevia powder in step 1), and step 1) 2) After the prepared extract extract is fully mixed, put the mixture into a sterilizing and drying equipment, first sterilize at high temperature, then quickly cool down to below 80°C, dry at low temperature, pass through an 80-mesh sieve for granulation, and pack separately Into 1000 bags, that is.

Usage and dosage: 1 bag each time, 2-3 times a day.

Embodiment 9: tea bag and preparation

Recipe: Snow Chrysanthemum 3kg, Kudingcha 2kg, Rattan Tea 0.5kg, Stevia 0.5kg

Preparation method: 1) Take snow chrysanthemum and stevia in the formula amount, vacuum-dry at 6°C until the water content is less than 5%, pulverize into fine powder, pass through a 60-mesh sieve for later use;

2) Take Kudingcha with 70% of the formula and crush it into a coarse powder with a particle size of 0.2-0.3 mm. Add 10 times the amount of Kudingcha and the branches and leaves of vine tea with the formula of 55% ethanol solution for reflux extraction for 2 hours , combine the extracts, filter, and concentrate to make an extract with a relative density of 1.20-1.30 at 55-60°C;

3) Turn on the frying pot, adjust the temperature to 85°C, and the speed of the stirring rod to 120 rpm, put in the coarse powder of Kudingcha and stir-fry for 20 minutes, then add the snow chrysanthemum powder and stevia powder in step 1), and step 1) 2) After the prepared extract extract is fully mixed, put the mixture into a sterilizing and drying equipment, first sterilize at high temperature, then quickly cool down to below 80°C, dry at low temperature, pass through an 80-mesh sieve for granulation, and pack separately Into 1000 bags, that is.

Embodiment 10: High formula quantity tablet

Recipe: Snow Chrysanthemum 7kg, Kudingcha 7kg, Rattan Tea 5kg, Stevia 3kg

Preparation method: with embodiment 1.

Example 11: Low formulation dosage tablet

Recipe: Snow Chrysanthemum 0.5kg, Kudingcha 0.5kg, Rattan Tea 0.3kg, Stevia 0.1kg

Preparation method: with embodiment 1.

Claims (13)

1. a compound recipe snow chrysanthemum product, it is characterized in that, described product is made up of the raw material of following weight portion: snow chrysanthemum 0.8-6 part, Folium Ilicis 0.8-6 part, Ampelopsis grossedentata 0.5-4 part, Folium Stevlae Rebaudianae 0.2-2 part.

2. compound recipe snow chrysanthemum product as claimed in claim 1, it is characterized in that, the parts by weight of raw materials of described product is: snow chrysanthemum 1.5-3 part, Folium Ilicis 1.5-3 part, Ampelopsis grossedentata 1-2 part, Folium Stevlae Rebaudianae 0.5-1 part.

3. compound recipe snow chrysanthemum product as claimed in claim 1 or 2, it is characterized in that, described product is medicine, health product or food.

4. compound recipe snow chrysanthemum product as claimed in claim 3, it is characterized in that, the dosage form of described medicine or health product is tablet, capsule, granule or oral liquid.

5. compound recipe snow chrysanthemum product as claimed in claim 3, it is characterized in that, the kind of described food is tea beverage, teabag.

6. compound recipe snow chrysanthemum product as claimed in claim 4, it is characterized in that, the preparation method of described compound recipe snow chrysanthemum medicine or health product comprises the following steps:

1) inulin will be avenged broken, for subsequent use with extraction by steam distillation volatile oil; The concentration of medicinal residues and leaf of Folium Stevlae Rebaudianae 6-10 times amount is alcohol reflux 1-3 time of 65%-100%, each 0.5-1.5 hour, extracting liquid filtering, and filtrate merges, and concentrated to be formed in relative density at 55-60 DEG C be the extractum of 1.15-1.30;

2) Ampelopsis grossedentata, Folium Ilicis is got, by water boiling and extraction 2-4 time of 6-10 times amount, each 1-3 hour, extracting liquid filtering, filtrate merges, and concentrated to be formed in relative density at 55-60 DEG C be the extractum of 1.10-1.30;

3) by the extract extractum of aforementioned obtained snow chrysanthemum, Folium Stevlae Rebaudianae, Ampelopsis grossedentata and Folium Ilicis, spraying dry or directly add the snow chrysanthemum volatile oil extracted, adds pharmaceutic adjuvant, makes required tablet, capsule, granule, oral liquid.

7. compound recipe snow chrysanthemum product as claimed in claim 6, is characterized in that, the preparation method of described compound recipe snow chrysanthemum tablet is:

1) inulin will be avenged broken, for subsequent use with extraction by steam distillation volatile oil; The concentration of medicinal residues and leaf of Folium Stevlae Rebaudianae 6-10 times amount is alcohol reflux 1-3 time of 20%-50%, each 0.5-1.5 hour, extracting liquid filtering, and filtrate merges, and concentrated to be formed in relative density at 55-60 DEG C be the extractum of 1.15-1.30;

2) Ampelopsis grossedentata, Folium Ilicis is got, by water boiling and extraction 2-4 time of 6-10 times amount, each 1-3 hour, extracting liquid filtering, filtrate merges, and concentrated to be formed in relative density at 55-60 DEG C be the extractum of 1.10-1.30;

3) by the extract extractum of aforementioned obtained snow chrysanthemum, Folium Stevlae Rebaudianae, Ampelopsis grossedentata and Folium Ilicis, carry out spraying dry, add the microcrystalline Cellulose of tablet total amount 15-20% in medicated powder after the drying, all the other are supplemented to full dose with pregelatinized Starch, add the snow chrysanthemum volatile oil collected, be mixed evenly, granulate with 8% starch slurry, dry, add the magnesium stearate of tablet total amount 0.5-1%, tabletting, to obtain final product.

8. compound recipe snow chrysanthemum product as claimed in claim 6, is characterized in that, the preparation method of described compound recipe snow chrysanthemum capsule agent is:

1) inulin will be avenged broken, for subsequent use with extraction by steam distillation volatile oil; The concentration of medicinal residues and leaf of Folium Stevlae Rebaudianae 6-10 times amount is alcohol reflux 1-3 time of 20%-50%, each 0.5-1.5 hour, extracting liquid filtering, and filtrate merges, and concentrated to be formed in relative density at 55-60 DEG C be the extractum of 1.15-1.30;

2) Ampelopsis grossedentata, Folium Ilicis is got, by water boiling and extraction 2-4 time of 6-10 times amount, each 1-3 hour, extracting liquid filtering, filtrate merges, and concentrated to be formed in relative density at 55-60 DEG C be the extractum of 1.10-1.30;

3) by the extract extractum of aforementioned obtained snow chrysanthemum, Folium Stevlae Rebaudianae, Ampelopsis grossedentata and Folium Ilicis, carry out spraying dry, add the microcrystalline Cellulose of capsule total amount 12-15% in medicated powder after the drying, all the other are supplemented to full dose with corn starch, add the snow chrysanthemum volatile oil collected, be mixed evenly, granulate, dry, add the magnesium stearate of total amount 0.5-1%, encapsulated after mixing, to obtain final product.

9. compound recipe snow chrysanthemum product as claimed in claim 6, is characterized in that, the preparation method of described compound recipe snow chrysanthemum granules agent is:

1) inulin will be avenged broken, for subsequent use with extraction by steam distillation volatile oil; The concentration of medicinal residues and leaf of Folium Stevlae Rebaudianae 6-10 times amount is alcohol reflux 1-3 time of 20%-50%, each 0.5-1.5 hour, extracting liquid filtering, and filtrate merges, and concentrated to be formed in relative density at 55-60 DEG C be the extractum of 1.15-1.30;

2) Ampelopsis grossedentata, Folium Ilicis is got, by water boiling and extraction 2-4 time of 6-10 times amount, each 1-3 hour, extracting liquid filtering, filtrate merges, and concentrated to be formed in relative density at 55-60 DEG C be the extractum of 1.10-1.30;

3) by the extract extractum of aforementioned obtained snow chrysanthemum, Folium Stevlae Rebaudianae, Ampelopsis grossedentata and Folium Ilicis, carry out spraying dry, add snow chrysanthemum volatile oil, the dextrin collected in medicated powder after the drying, mixing, granulate, dry, granulate, packaging, obtains granule.

10. compound recipe snow chrysanthemum product as claimed in claim 6, is characterized in that, the preparation method of described compound recipe snow chrysanthemum oral liquid is:

1) inulin will be avenged broken, for subsequent use with extraction by steam distillation volatile oil; The concentration of medicinal residues and leaf of Folium Stevlae Rebaudianae 6-10 times amount is alcohol reflux 1-3 time of 20%-50%, each 0.5-1.5 hour, extracting liquid filtering, and filtrate merges, and concentrated to be formed in relative density at 55-60 DEG C be the extractum of 1.15-1.30;

2) Ampelopsis grossedentata, Folium Ilicis is got, by water boiling and extraction 2-4 time of 6-10 times amount, each 1-3 hour, extracting liquid filtering, filtrate merges, and concentrated to be formed in relative density at 55-60 DEG C be the extractum of 1.10-1.30;

3) by the extract extractum of aforementioned obtained snow chrysanthemum, Folium Stevlae Rebaudianae, Ampelopsis grossedentata and Folium Ilicis, add water to 50% of total amount, add the snow chrysanthemum volatile oil of collection, mixing, simple syrup adds to sugar content 15-20%, add 0.1% sodium benzoate, adding distil water is to full dose, and mixing, filters, embedding, obtains oral liquid.

11. compound recipe snow chrysanthemum products as claimed in claim 5, is characterized in that, the preparation method of described compound recipe snow chrysanthemum tea beverage comprises following steps:

1) will avenge chrysanthemum, leaf of Folium Stevlae Rebaudianae is pulverized, be alcohol reflux 1-3 time of 20%-50% by the concentration of 6-10 times amount, each 0.5-1.5 hour, extracting liquid filtering, and filtrate merging is for subsequent use;

2) Ampelopsis grossedentata, Folium Ilicis is got, by water boiling and extraction 2-4 time of 6-10 times amount, each 1-3 hour, extracting liquid filtering, filtrate merges, for subsequent use;

3) by step 1 and 2 obtained extract solutions, add the chitosan of crude drug amount 1%, leave standstill 24 hours, filter, filtrate adds water, and stirs evenly, and adjust ph, to 4-5, adds the crystal sugar of beverage total amount 6-9%, the citric acid of 0.05-0.12%, aseptic filtration, filtrate subpackage, rolls lid, makes beverage.

12. compound recipe snow chrysanthemum products as claimed in claim 5, it is characterized in that, the preparation method of described compound recipe snow chrysanthemum bagged tea comprises following steps:

1) get the snow chrysanthemum of formula ratio, Folium Stevlae Rebaudianae, at 0-6 DEG C, vacuum drying is less than 5% to water content, is ground into fine powder, crosses 60 mesh sieves for subsequent use;

2) lectuce tea powder getting formula ratio 50%-70% is broken into the coarse powder that granularity is 0.2-0.3mm, the Ampelopsis grossedentata branch and leaf of all the other Folium Ilicis and formula ratio, add 6-10 times amount, concentration is 55%-75% alcoholic solution reflux, extract, 1-2 time, each 1-2 hour, merge extractive liquid, filter, concentrating and being formed in relative density at 55-60 DEG C is the extractum of 1.20-1.30;

3) open parch pot, adjustment temperature is 75-85 DEG C, and stirring rod speed is 80-120 rev/min, put into after Folium Ilicis coarse powder stir-fries 10-20 minute, add step 1) snow inulin and Folium Stevlae Rebaudianae powder, and step 2) obtained extract extractum, after abundant mixing, mixture is put into sterilizing, drying equipment, first high temperature sterilize, be then cooled to rapidly less than 80 DEG C, cold drying, cross 80 mesh sieve granulate, subpackage, to obtain final product.

Compound recipe snow chrysanthemum product described in 13. any one of claim 1-12 has blood pressure lowering, blood sugar lowering and blood fat reducing three in preparation and falls application in functional product.