Garden waste treatment process and uses thereof
Technical field
The present invention relates to the agricultural wastes processing technology field, especially garden waste treatment process, and the purposes of the product that obtains.
Background technology
Along with the development of Urbanization in China, afforestation more and more obtains people's attention in the vital role of improving city environmental quality, alleviating urban heat island effect, keep aspects such as balance of city ecosystem, and is able to fast development; But consequent tree limb, deadwood, fallen leaves, grass bits, flower loses and afforestation waste such as other greening lop is also sharply increasing; Its processing mode has become the problem that can not be ignored in the modern metropolitan cities development, and the afforestation changing rejected material to useful resource utilizes Study on Key Technique and Industry Promotion demonstration to become the focus that everybody pays close attention to again.
Early the beginning is carried out compost treatment to garden waste in part developed country; On Canada, the U.S. and other places; Because scarcely populated, often take the Recycle design of floor pavement let garden waste natural degradation, just directly cover carrying out ground after branch, the fallen leaves pulverization process.The permanent dung yard area that New York Botanical Garden sets up reaches 2787m
2, it is half that the rubbish of handing the processing of environmental sanitation office over to has been reduced.In Europe, the management of city trees and shrubs waste has strict Regulatory control.Landfill method like European Union has qualification to the ratio that organic waste gets into landfill yard, and reduces ratio year by year; Nitrogen method strict regulation land area of one unit chemical fertilizer usage quantity, effectively promoted the market development of organic compost.Brussels has 15 large-scale open-air dung yards, settles the field for 4.In Japan, take multiple compost method to handle the greening waste; The bark pile fermentation is made fertilizer; It is fertile to use disintegrating apparatus movably that laggard windrow is pulverized in the beta pruning of trees etc. on the spot; Encourage the active compost of family manufacture; All kinds of starters and small-sized compost supporting machinery A wide selection of colours and designs, many greenery patchess, gardens all have supporting dung yard.
In order to promote the afforestation waste recycle; The Ministry of Construction of the People's Republic of China, MOC pointed out " branch, the minimizing of encouraging to handle pruning through modes such as compost, development biomass material, organotrophy matrix and deep processings take garbage loading embeading storage capacity, realize recycle " on August 30th, 2007 in " about building the suggestion of resource-saving city's afforestation ".In recent years; Domestic garden waste correlative study with utilize technology that bigger development has been arranged, can garden waste be pulverized fermentation and make matrix or biological organic fertilizer, pulverize and cover again and utilize, make edible fungus stick, produce and process biomass energy etc. on the spot.In Beijing, ground such as Guangzhou and Shanghai have large-scale branch and pulverize, it is fertile that the branches and leaves of collecting, turf-mown thing etc. are pulverized laggard windrow, processes commodity and be used for afforestation.
But present existing agriculture and forestry organic waste material composting technology is produced fertilizer, consumption is big, fertilizer efficiency is slow, is worth not highly, and manufacturing enterprise's benefit is undesirable.
The research that utilizes mikrobe to protect seed in germination process, to exempt from the phytopathogen infringement receives people's attention always.At present, have 6 national registration registration fungus Trichoderma class biotechnological formulations at least, handle several kinds of crop seeds with Trichoderma and all improved the output of crop.
The both effective controlling plant diseases of the rhizosphere bacterium Bacillus subtilis GBO3 that U.S.'s Alabama agricultural experiment station is found promotes plant-growth again, has obtained large scale application (commodity are called Kodiak) at US and European.
A kind of Latin formal name used at school is that the bacterium of " Pseudomonas chlororaphis " is used as seed treatment agent by EU Committee's approval.The commodity Cedomon by name that contains organic seed treatment agent of this material.A kind of Latin formal name used at school is that the bacterium of " Pseudomonas chlororaphis " is used as seed treatment agent by EU Committee's approval.The commodity Cedomon by name that contains organic seed treatment agent of this material.
Root nodule bacterium are as the artificial inoculation thing, and prolonged application is in legume-seeds.The inoculum of root nodule bacterium as fertilizer sources a kind of surrogate and come into the market.
Summary of the invention
The present invention is directed to deficiency, propose a kind of treatment process of garden waste, waste is effectively utilized, produced high economic benefit.
In order to realize the foregoing invention purpose, the present invention provides following technical scheme: the treatment process of garden waste may further comprise the steps:
1., garden waste is carried out categorised collection, distinguish draft, broad-leaved and this raw material of needlebush, select to use draft and the broadleaf woody plants waste that does not have volatilization fragrance, carry out coarse reduction and the secondary fine powder is broken;
2., get step 1. garden waste powder, cotton benevolence cake powder, Semen Maydis powder, wheat bran, the peat composed of rotten mosses and loam; By weight being 60~80: 5~8: 6~10: 12~20: 4~8: 20~40; Pulverize mixing, add water to 25~30% of gross weight, adjust pH to 7.5~8.5;
3., getting step 2. handles the mix material, sterilization; Add streptomyces microflavus, carried out fermentative processing 5~8 days in 26~32 ℃.
Preferably, said streptomyces microflavus is that following manner is cultivated making:
(1) slant strains is cultivated:
Culture medium prescription: potato 20~30%, glucose 2~4%, agar 2~4%; With the potato decortication section of cleaning, add water and boil clay coating earlier, use filtered through gauze; Add agar and glucose, stir nutrient solution; Adjust pH to 7.0~7.5, sterilization;
Rearmounted 24~32 ℃ of heat insulating culture of inoculation 10~15 days;
(2) shake a bottle strain cultivation:
Sucrose, Semen Maydis powder, soybean cake powder, cottonseed meal, lime carbonate concentration are respectively 1~3%, mix, soluble in water; Regulate pH value 8~8.5; In 28~32 ℃, be put on 120~225 rev/mins of shaking tables shaking culture at least 30 hours; Inspection does not have assorted bacterium peculiar smell, smear for microscopic examination, and to reach mycelia evenly sturdy and do not have autolysis, subsequent use.
A kind of garden waste fertilizer, the treatment process of garden waste makes as stated.
A kind of garden waste fertilizer is used for the seeding treatment agent.
Compared with prior art, the present invention is a main raw material with the agriculture and forestry organic waste material, with utilization of waste as resource, greatly reduces cost; With tunning seeding treatment agent, improved added value of product, produced high economic benefit.Liquid fermentation technology is adopted in second class inoculum production of the present invention, has practiced thrift the time, has reduced cost.
The present invention utilizes agriculture and forestry organic waste material seeding treatment agent with the changing rejected material to useful resource recycle, and the seed treatment agent of production can germinate for seed provides fertilizer efficiency, disease-resistant expelling parasite, hestening rooting, and Miao Qimiao is strong, for high grade and yield of crops lays the foundation.Cost of the present invention is low, is worth height, and consumption is few, is 10% of mu sowing quantity.
The tunning of the garden waste that the present invention obtains has following effect:
1. fertility effect: the structure of effectively improving the soil and micro-ecological environment, increase the humus content of the soil, play the effect of culture fertility; Can not be by elements such as the nitrogen of plant absorbing, phosphorus in the soil, being converted into can be by the nutrient of plant absorbing.
This microbial inoculum is a main medium matter with garden waste, cake, and raw material itself can provide multiple nutrients such as nitrogen, phosphorus, potassium; The Feng Yi bacterium produces organic acid, can nutrients such as rhizosphere soil part insoluble phosphorus be discharged.Make loosing soil, reduce soil compaction, help water conservation, protect fertilizer, ventilate and promote root system development, for farm crop provide comfortable growing environment.
2. the diseases prevention anthelmintic action of keeping a full stand of seedings.The product that obtains has preventive and therapeutic effect to fungoid, bacterial disease, and viral disease is had restraining effect.This bacterium can secrete two kinds of microbiotic that can suppress or kill and wound pathomycete and bacterium respectively.Mashed kind of Achlya spp, cotton and wheat etc. there are significantly control effect and the harm that can alleviate 30 multiple diseases such as cotton in seedling stage root rot, verticillium, sweet potato black rot, wheat rust, rice sheath blight disease, bacterial leaf spot and rice blast to some extent; Insects such as cutworm there is repellent action.
3. stimulating growth effect.Contain type material such as growth hormone, phytokinin, Plant hormones regulators,gibberellins, microbiotic, toluylic acid, the acid of coral amber and multiple amino acids in its meta-bolites.Have breaking dormancy, promote seed germination, suppress seed and carry germ percentage of germination, hestening rooting, stimulating plant cell fission take place, improve, improve chlorophyll content, strengthen photosynthetic efficiency, promote effects such as protein synthesis.
Embodiment
Describe the present invention below in conjunction with specific embodiment, the description of this part only is exemplary and explanatory, should any restriction not arranged to protection scope of the present invention.
The treatment process of garden waste comprises following process: garden waste is carried out categorised collection, distinguish draft, broad-leaved and this raw material of needlebush, the draft and the broadleaf woody plants waste of the fragrance of selecting not volatilize, carry out coarse reduction and the secondary fine powder is broken; Prescription carries out the substratum preparation.
Production technique is following:
(1) slant strains is cultivated
1. substratum
Prescription: potato 20%, glucose 2%, agar 2%.With the potato decortication of cleaning, thinly slice earlier, weigh 200 grams, add 1000 milliliters in water, boil into clay coating, use filtered through gauze; After supplying the moisture that loses, weigh agar 20 gram, add and fully melt with little fire; Add glucose 10-20 gram again, stir nutrient solution; Test pH value then, reach 7.5.
The dress test tube: every the test tube of packing into while hot, the amount of packing into are about about 1/4 of test tube cubic capacity.Charging method connects rubber tubing, cuvette down with bigger funnel, and the centre adds spring pinchcock with dominant discharge and prevent to solidify.The cotton plug that the test tube mouth is filled in, the length of filling in are 3/5ths of cotton plug total length.
Medium sterilization: test tube is pressed bundled, cotton part was wrapped with newspaper, puts into high-pressure sterilizing pot, 1.1 kilograms per centimeter, 2 sterilizations 30-40 minute.After the sterilization, be depressurized to zero.The taking-up of uncapping is tiltedly put while hot.Generally making the inclined-plane is that 1/2 of test tube total length is advisable.Cooling back slant medium.
2. inoculation
Transfer room should be selected bright and clear, the houselet of air seasoning.Inoculation is closed the doors and windows previous day, uses the Superlysoform suffocating sterilization.Before the inoculation things such as substratum and bacterial classification are taken into indoor, phenylic acid with 5% or 0.1% benzalkonium bromide spray the room.Former bacterial classification inserts on the test tube slant with the sand pipe on intensive Alcohol Flame side with transfering loop.Inoculate rearmounted 24-32 ℃ heat insulating culture 10-15 days.
3. cultivate
Postvaccinal test tube is placed in 28 ℃ of greenhouses and cultivated 5-10 days, promptly on the inclined-plane, covers with whitewash band blush spore layer.Temporary transient time spent not should be placed in the case or cupboard of clean, cleaning, covers with newspaper.
Quality inspection: normal strain inclined plane, should grow up to the little red spore layer of whitewash band, produce little dewdrop on it, give out the refrigerant fragrance of borneol.Redder with color, dewdrop is more, the spore layer is thicker for well.
(2) shake the method for making of bottle liquid spawn
Sucrose, Semen Maydis powder, soybean cake powder, cottonseed meal, lime carbonate respectively 1% are mixed in 100 ml waters; Regulate pH value 8-8.5, sterilization is 7-7.5 afterwards, in 500 milliliters the triangular flask of packing into then; Autoclaving 30 minutes; Put into sterilisable chamber while hot, when being chilled to 30 ℃, in Bechtop, insert one of slant tube bacterial classification on the spirit lamp flame.
In 28-32 ℃, be put on the 120-225 rev/min of shaking table, shaking culture is after 30 hours, and inspection does not have assorted bacterium peculiar smell, and it is evenly sturdy that smear for microscopic examination reaches mycelia, and behind the no autolysis, it is subsequent use to put into refrigerator.
(3) koji tray fermentative prodn
Prescription: garden waste, cotton benevolence cake powder, Semen Maydis powder, wheat bran, the peat composed of rotten mosses and loam etc. are pulverized by a certain percentage, sieve, are mixed thoroughly, and adding moisture is about 25%, transfer pH value, reach between the 7.5-8.5.Cake soil 1/2 to 3/4 of the container of packing into is advisable, seals and tighten with cotton cord with newspaper.
Inoculation culture: 1.1 kilograms per centimeter sterilization 1.5 hours.When taking-up waits to expect temperature drop to non-scald on hand (45-50 ℃), inoculate.Remain on after the inoculation between 26-32 ℃.Be placed on draughty place, grow basically about 5 days.
This bacterium can grow rapidly in soil surely, and the active spore of Feng Yi bacterium is a large amount of breedings around seed, reduces seed and receives the infringement of germ, stimulates seed germination, takes root, emerges in advance, precocious, raising the output.
It below only is preferred implementation of the present invention; Should be pointed out that for those skilled in the art, under the prerequisite that does not break away from the principle of the invention; Can also make some improvement and retouching, these improvement and retouching also should be regarded as protection scope of the present invention.