CN104761385A - Multifunctional microbial organic fertilizer and preparation method thereof - Google Patents
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- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
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- WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 description 1
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Classifications
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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- Fertilizers (AREA)
Abstract
Description
一、技术领域 1. Technical field
本发明涉及的是植物生长过程中使用的生物肥料,具体涉及的是一种多功能微生物有机肥及其制备方法。 The invention relates to a biological fertilizer used in the process of plant growth, in particular to a multifunctional microbial organic fertilizer and a preparation method thereof.
二、背景技术 2. Background technology
由于我国农业的过度开发,尤其是长期偏施单质化肥,没有适当给土壤补充农业有机质,导致土壤质量下降,可利用的农业资源逐步枯竭,土壤退化严重,最终影响优质农产品的生产。我国目前的土壤资源现状迫切需要通过人为措施补充土壤农业有机质,确保农业种植水平和农作物产品品质。与这一形势相对应的是,我国有机肥技术的研发和推广相对落后。市场上可供选择的产品和有机肥生产厂家更是寥寥无几。例如,黑龙江省是我国大米出口的主要省份。多年来,省粮食出口部门遇到最头痛的问题就是,常常有成批出口的大米被国外质检机构返回。问题是因为在生产过程中长期大量化肥、农药的使用,使稻米的品质不能达到进口国的要求。另外由于随着农作物种植年限增长,导致土壤内积累了大量的有害生物,病害发生越来越重,土壤出现大量板结,肥力和耕力下降,最后导致农作物产量、品质大幅度下降。因此土壤肥力下降和土传病害已成为影响农作物生产和品质的重要限制因素。随着人们生活水平逐步提高与消费观念的升级,对绿色产品需求的提升以及国家对节能降耗的要求,发展绿色环保的微生物有机肥成为当前社会发展主流。然而目前微生物有机肥的发酵大部分采用好氧发酵,需要在发酵的同时不断对堆肥进行搅拌通氧,导致在发酵过程中恶臭外泄,严重污染环境和操作人员身体健康,并且增加了设备成本、能源成本、人力成本。综上所述,在研究本发明的过程中,发明人发现目前生产微生物有机肥存在以下问题: Due to the over-exploitation of my country's agriculture, especially the long-term partial application of chemical fertilizers, there is no proper supplement of agricultural organic matter to the soil, resulting in the decline of soil quality, the gradual depletion of available agricultural resources, and serious soil degradation, which ultimately affects the production of high-quality agricultural products. The current status of soil resources in my country urgently needs artificial measures to supplement soil agricultural organic matter to ensure the level of agricultural planting and the quality of crop products. Corresponding to this situation, the development and promotion of organic fertilizer technology in my country is relatively backward. There are very few products and organic fertilizer manufacturers to choose from on the market. For example, Heilongjiang Province is the main province of my country's rice export. Over the years, the most troublesome problem encountered by the provincial grain export department is that batches of exported rice are often returned by foreign quality inspection agencies. The problem is that the quality of rice cannot meet the requirements of importing countries due to the long-term use of large amounts of chemical fertilizers and pesticides in the production process. In addition, with the increase of crop planting years, a large number of harmful organisms have accumulated in the soil, the occurrence of diseases has become more and more serious, the soil has a large amount of compaction, and the fertility and tillage power have decreased, which eventually leads to a significant decline in crop yield and quality. Therefore, soil fertility decline and soil-borne diseases have become important limiting factors affecting crop production and quality. With the gradual improvement of people's living standards and the upgrading of consumption concepts, the increasing demand for green products and the country's requirements for energy saving and consumption reduction, the development of green and environmentally friendly microbial organic fertilizers has become the mainstream of current social development. However, at present, most of the fermentation of microbial organic fertilizers adopts aerobic fermentation, which needs to continuously stir and aerate the compost during fermentation, resulting in the leakage of stench during the fermentation process, seriously polluting the environment and the health of operators, and increasing the cost of equipment , energy costs, labor costs. In summary, in the process of researching the present invention, the inventor finds that the following problems exist in the production of microbial organic fertilizers at present:
1. 目前微生物有机肥的发酵大部分采用好氧微生物,因此在发酵过程需要进行通风供氧,需要进行多次翻堆搅拌作业,并且在操作过程中会产生恶臭,出现污水横流,造成能源的浪费,提高设备成本; 1. At present, aerobic microorganisms are mostly used in the fermentation of microbial organic fertilizers. Therefore, ventilation and oxygen supply are required during the fermentation process, and multiple turning and stirring operations are required. In addition, stench will be generated during the operation process, and sewage cross-flow will occur, resulting in energy loss. Waste, increase equipment cost;
2. 生产的微生物有机肥往往对土壤肥力和土壤结构有一定改善作用,但是对病害防治效果比较差,特别是对土传病害没有防治能力。 2. The microbial organic fertilizer produced often has a certain effect on improving soil fertility and soil structure, but the effect on disease control is relatively poor, especially for soil-borne diseases.
三、发明内容 3. Contents of the invention
本发明的目的是提供一种多功能微生物有机肥,这种多功能微生物有机肥用于解决现有技术中微生物有机肥发酵过程中恶臭外泄,严重污染环境及病害防治效果比较差的问题,本发明的另一个目的是提供这种多功能微生物有机肥的制备方法。 The object of the present invention is to provide a kind of multi-functional microbial organic fertilizer, this multi-functional microbial organic fertilizer is used to solve the problems of malodorous leakage in the microbial organic fertilizer fermentation process in the prior art, serious environmental pollution and relatively poor disease control effect, Another object of the present invention is to provide the preparation method of this multifunctional microbial organic fertilizer.
本发明采用的技术方案是:这种多功能微生物有机肥由发酵农业有机肥和中国专利ZL201210108437.8提供的多效生物药肥混合形成,上述两种组份按质量百分比计,发酵农业有机肥70-90%,多效生物药肥10-30%;发酵农业有机肥是使用兼性厌氧菌群种子发酵剂与农业有机废弃物原料混合后利用堆肥厌氧发酵方式产生的有机肥,农业有机废弃物原料由玉米秸杆、大豆秸杆、稻草、杂草、畜类粪便任意一种构成或其中任意几种任意混合形成; The technical solution adopted in the present invention is: this multifunctional microbial organic fertilizer is formed by mixing fermented agricultural organic fertilizer and the multi-effect biological medicinal fertilizer provided by Chinese patent ZL201210108437.8. 70-90%, multi-effect biomedical fertilizer 10-30%; fermented agricultural organic fertilizer is an organic fertilizer produced by composting anaerobic fermentation after mixing the seed starter of facultative anaerobic bacteria with agricultural organic waste raw materials. Organic waste raw materials are composed of any one of corn stalks, soybean stalks, rice straw, weeds, and animal manure, or any combination of any of them;
兼性厌氧菌群种子发酵剂制备如下: The preparation of the facultative anaerobic flora seed starter is as follows :
1)母菌群制备: 1) Preparation of mother flora:
将单菌落放线菌、酵母菌、链霉菌分别接种于固体酵母浸膏LB培养基上,在25℃温度下,培养1-3天,用无菌芽签分别挑取单菌落放线菌、酵母菌、链霉菌, 然后分别接种到酵母浸膏LB液体培养基中,180-220转/分,发酵温度25-28℃,发酵时间为2-6天,然后将发酵后的单菌落放线菌、酵母菌、链霉菌,按单菌落放线菌:酵母菌:链霉菌=3:3:4的比例进行混合,混合液即为母菌群; Inoculate single-colony actinomycetes, yeast and streptomyces on solid yeast extract LB medium respectively, culture at 25°C for 1-3 days, pick out single-colony actinomycetes, Saccharomyces and Streptomyces were then inoculated into yeast extract LB liquid medium, 180-220 rpm, fermentation temperature 25-28°C, fermentation time 2-6 days, and then the single colony after fermentation was released Bacteria, yeast, streptomyces, mixed according to the ratio of single colony actinomycetes: yeast: streptomyces = 3:3:4, the mixture is the mother flora;
2)堆肥菌群种子发酵剂的制备: 2) Preparation of compost flora seed starter:
①将步骤1)制备的母菌群原液按体积比1:150-200加入到无菌液体酵母浸膏培养基中,培养温度40-50℃,180rpm振荡培养24h; ①Add the stock solution of mother flora prepared in step 1) into the sterile liquid yeast extract medium at a volume ratio of 1:150-200, culture at 40-50°C, and shake at 180rpm for 24 hours;
②将干稻草秸秆剪成约3cm长,把剪短的干稻草与水按质量/体积比10:4混合,振荡混匀,121℃,20min灭菌,即得浸湿稻草; ②Cut the dry rice straw into about 3cm long, mix the cut dry rice straw with water at a mass/volume ratio of 10:4, shake and mix, sterilize at 121°C for 20 minutes, and then soak the straw;
③取上述步骤①的培养液按体积比1: 400-800加入无菌水中,摇匀后按体积比1:10-20加入到灭菌后的上述步骤②的浸湿稻草中,振荡搅拌均匀,50℃培养7-10天,即兼性厌氧菌群种子发酵剂。 ③ Take the culture solution of the above step ① and add it to the sterile water according to the volume ratio of 1: 400-800, shake well and add it to the soaked straw in the above step ② after sterilization according to the volume ratio of 1: 10-20, shake and stir evenly , Cultivated at 50°C for 7-10 days, that is, the seed starter of facultative anaerobic flora.
上述多功能微生物有机肥的制备方法: The preparation method of above-mentioned multifunctional microbial organic fertilizer:
一、制备兼性厌氧菌群种子发酵剂; One, preparation of facultative anaerobic flora seed starter;
二、准备农业有机废弃物原料; 2. Prepare agricultural organic waste raw materials;
三、堆肥制备发酵农业有机肥: 3. Preparation of fermented agricultural organic fertilizer by composting:
1. 测量地面面积,至少保证45立方的废弃物原料堆高1.5米以上,初步设定地面面积为15平方米,在其上面铺一层薄膜,然后在薄膜上撒一薄层兼性厌氧菌群种子发酵剂; 1. Measure the ground area to ensure that at least 45 cubic meters of waste materials are piled up to a height of more than 1.5 meters. The ground area is initially set to 15 square meters, and a layer of film is spread on it, and then a thin layer of facultative anaerobic is sprinkled on the film. Bacteria seed starter;
2. 在兼性厌氧菌群种子发酵剂上铺厚约30-40cm的农业有机废弃物原料,并加水充分拌匀,使含水量为55-65%,然后在其上撒一层菌群种子发酵剂,以此类推,直到原料堆高1.5米以上; 2. Spread about 30-40cm thick agricultural organic waste raw materials on the facultative anaerobic flora seed starter, add water and mix well to make the water content 55-65%, and then sprinkle a layer of flora on it Seed starter, and so on, until the raw material is more than 1.5 meters high;
3. 在原料堆上覆盖一层雨布并压实,用温度计测定东西南北4个方位的温度并作记录; 3. Cover the raw material pile with a layer of rain cloth and compact it, and use a thermometer to measure and record the temperature in four directions;
4. 直到堆肥50-90天后掀开雨布,通过混匀即得到发酵农业有机肥; 4. Remove the rain cloth after composting for 50-90 days, and get fermented agricultural organic fertilizer by mixing;
四、将步骤三得到的发酵农业有机肥和中国专利ZL201210108437.8提供的多效生物药肥混合,混合时按质量百分比计,发酵农业有机肥70-90%,多效生物药肥10-30%,即得到多功能微生物有机肥。 4. Mix the fermented agricultural organic fertilizer obtained in step 3 with the multi-effect biomedical fertilizer provided by Chinese patent ZL201210108437.8. When mixing, the fermented agricultural organic fertilizer is 70-90% and the multi-effect biomedical fertilizer is 10-30%. %, to obtain multifunctional microbial organic fertilizer.
上述方案中多功能微生物有机肥用1-50公斤塑料包装袋包装。 In the above scheme, the multifunctional microbial organic fertilizer is packed in 1-50 kg plastic bags.
上述方案中农业有机废弃物原料由按质量百分比计的玉米秸杆50%和牛粪50%混合形成。 In the above scheme, the agricultural organic waste raw material is formed by mixing 50% corn stalks and 50% cow dung by mass percentage.
上述方案中农业有机废弃物原料由按质量百分比计的稻草秸杆50%和牛粪50%混合形成。 In the above scheme, the raw material of agricultural organic waste is formed by mixing 50% of rice straw and 50% of cow dung by mass percentage.
上述方案中农业有机废弃物原料由按质量百分比计的稻草秸杆70、杂草10%、牛粪20%混合形成。 In the above scheme, the raw material of agricultural organic waste is formed by mixing 70% of rice straw, 10% of weeds, and 20% of cow dung by mass percentage.
有益效果: Beneficial effect:
1、养分全面: 1. Comprehensive nutrition:
有机肥是一种完全肥料,含有农作物生长发育所需要的丰富的碳、氮、磷、钾、钙、镁、硫、硅等大中量元素和一定量的铁、铜、锌、锰、硼、钼等微量元素。 Organic fertilizer is a kind of complete fertilizer, which contains abundant carbon, nitrogen, phosphorus, potassium, calcium, magnesium, sulfur, silicon and other large and medium elements and a certain amount of iron, copper, zinc, manganese, boron which are needed for the growth and development of crops. , molybdenum and other trace elements.
2、改良土壤: 2. Improve the soil:
有机肥含有大量有机质,长期施用能提高土壤有机质,改善土壤团粒结构,促进土壤微生物繁殖,使土壤疏松、通气,易于排水、耕作。 Organic fertilizer contains a large amount of organic matter. Long-term application can increase soil organic matter, improve soil aggregate structure, promote soil microbial reproduction, make soil loose, ventilated, and easy to drain and cultivate.
3、保肥保水: 3. Fertility and water retention:
施用有机肥可增强土壤的保肥性能和缓冲力。腐殖质为有机胶体,颗粒虽小,但表面积大,吸收水分能力比无机胶体大100多倍,代换量大4倍。长期施用有机肥,可使有机、无体复合胶体增多,土壤融合密切,增强保水、保肥、供肥能力。 Applying organic fertilizers can enhance soil fertility and buffering capacity. Humus is an organic colloid. Although the particles are small, it has a large surface area. Its water absorption capacity is more than 100 times greater than that of inorganic colloids, and its substitution capacity is 4 times greater. Long-term application of organic fertilizer can increase the amount of organic and non-body compound colloids, closely integrate the soil, and enhance the ability of water retention, fertilizer retention, and fertilizer supply.
4、防治病虫害: 4. Prevention and control of pests and diseases:
有机肥在发酵腐熟过程中,已经杀死很多杂草种子、害虫虫卵、致病菌的孢子及繁殖体等,并且与一种多效生物药肥混合后,在后期施用时其微生物菌种可以再次发酵产生大量生防木霉菌,故在作物上时可以在一定程度上防止病虫害的发生,并且对土传性病害有较好的防治作用。 During the fermentation and decomposing process of organic fertilizer, many weed seeds, pest eggs, spores and propagules of pathogenic bacteria have been killed, and after being mixed with a multi-effect biological fertilizer, the microbial strains in the later application It can be fermented again to produce a large amount of biocontrol Trichoderma, so it can prevent the occurrence of diseases and insect pests to a certain extent when it is used on crops, and has a good control effect on soil-borne diseases.
5、改善农产品品质: 5. Improve the quality of agricultural products:
鉴于本产品养分全,松散无恶臭,故有很多应用。现在很多城市居民在工作之余尽可能利用家中有限的自留地发展一些蔬菜种植,比如夏天用泡沫盒子种一些空心菜,苋菜,然后到了冬天就换成芫荽或者白菜,有的还会搭上架子种上一些丝瓜黄瓜之类的藤蔓类蔬菜,到了盛夏枝繁叶茂之时,也构成了一道独特的景观。然而,随着科学技术的发展以及生活水平的提高,这种阳台种植的缺点越来越凸显。最明显的就是多半居民都是施用沤肥一类肮脏并且带有强烈异味的肥料,对于那些喜欢室内栽培的居民,是绝对不可取的。但是用该发酵方法生产的有机肥却可以有效避免这一问题。首先是没有臭味,其次是这种有机肥被充分腐熟,有机质得到很好的分解,养分全,有利于作物快速吸收;有机肥中的有益微生物还可以改善土壤状况,预防很多作物病害的发生,减少农药的使用,使种植的蔬菜瓜果更健康。 In view of the fact that this product is full of nutrients, loose and odorless, it has many applications. Nowadays, many urban residents try their best to use the limited private land at home to grow some vegetables after work. For example, they use foam boxes to grow some water spinach and amaranth in summer, and then replace them with coriander or cabbage in winter, and some even put them on shelves. Some vine vegetables such as loofah and cucumber also form a unique landscape when they are in full bloom in midsummer. However, with the development of science and technology and the improvement of living standards, the shortcomings of this balcony planting have become more and more prominent. The most obvious thing is that most of the residents use retting fertilizers, which are dirty and have a strong odor, which are absolutely not advisable for those residents who like indoor cultivation. However, the organic fertilizer produced by this fermentation method can effectively avoid this problem. The first is that there is no odor, and the second is that this organic fertilizer is fully decomposed, the organic matter is well decomposed, and the nutrients are complete, which is conducive to the rapid absorption of crops; the beneficial microorganisms in the organic fertilizer can also improve the soil condition and prevent the occurrence of many crop diseases. , reduce the use of pesticides, and make the grown vegetables and fruits healthier.
不仅在家庭种植上有广泛的应用,在其余作物上该有机肥的效果也很好。应用在花卉上时,使花卉枝茎茁壮、颜色鲜艳;应用在瓜果类蔬菜及果树上可以充分提高坐果率,果型好,个头大,甜度高,有效地提高亩产;应用在叶菜类蔬菜上,使其叶片肥大、翠绿,明显提高产量,同时蔬菜口感鲜嫩、味道鲜美,营养丰富;应用在根茎类蔬菜上,使根茎硕大,提高量产,同时有效地预防土传病害的发生,减少农药使用。 Not only is it widely used in home planting, but the organic fertilizer also works well on other crops. When applied to flowers, it can make the branches and stems of flowers strong and bright in color; when applied to melons, fruits, vegetables and fruit trees, it can fully increase the fruit setting rate, the fruit shape is good, the size is large, and the sweetness is high, effectively increasing the yield per mu; when applied to leaves Applied to vegetable vegetables to make the leaves plump and emerald green, significantly increasing the yield, and meanwhile the vegetables taste fresh and tender, delicious and rich in nutrition; applied to rhizome vegetables to make the rhizomes huge, increase mass production, and effectively prevent soil-borne diseases at the same time occur and reduce the use of pesticides.
四、具体实施方式 4. Specific implementation
下面对本发明做进一步的说明: The present invention is further described below:
实施例1Example 1
这种多功能微生物有机肥由发酵农业有机肥和中国专利ZL201210108437.8提供的多效生物药肥混合形成,上述两种组份按质量百分比计,发酵农业有机肥70%,多效生物药肥30%;发酵农业有机肥是使用兼性厌氧菌群种子发酵剂与农业有机废弃物原料混合后利用堆肥厌氧发酵方式产生的有机肥,农业有机废弃物原料由玉米秸杆和牛粪混合形成。 This multifunctional microbial organic fertilizer is formed by mixing fermented agricultural organic fertilizer and multi-effect biomedical fertilizer provided by Chinese patent ZL201210108437.8. 30%; fermented agricultural organic fertilizer is an organic fertilizer produced by using facultative anaerobic flora seed starter mixed with agricultural organic waste raw materials and using compost anaerobic fermentation. The agricultural organic waste raw materials are formed by mixing corn stalks and cow dung .
一、兼性厌氧菌群种子发酵剂的制备1. Preparation of facultative anaerobic flora seed starter
1)母菌群制备 1) Preparation of mother flora
将放线菌, 酵母菌, 链霉菌分别接种于固体酵母浸膏(LB)培养基上,在25℃温度下,培养1-3天,用无菌芽签分别挑取单菌落放线菌, 酵母菌, 链霉菌, 然后分别接种到酵母浸膏(LB)液体培养基三角瓶中,180转/分,发酵温度25℃,发酵时间为3天, 然后按比例为放线菌: 酵母菌: 链霉菌=3: 3: 4进行混合,混合液为母菌群; Inoculate Actinomycetes, Yeast, and Streptomyces on solid yeast extract (LB) medium, culture at 25°C for 1-3 days, and pick single colonies of Actinomycetes with sterile bud sticks. Saccharomyces, Streptomyces, and then respectively inoculated into yeast extract (LB) liquid medium Erlenmeyer flask, 180 rpm, fermentation temperature 25°C, fermentation time is 3 days, and then actinomycetes in proportion: Yeast: Streptomyces=3: 3: 4 is mixed, and mixed solution is parent flora;
2)堆肥中兼性厌氧菌群种子发酵剂的制备 2) Preparation of facultative anaerobic flora seed starter in compost
①将上述的母菌群原液按体积比(v/v)1:150加入到无菌液体酵母浸膏培养基,培养温度40℃,180rpm振荡培养24h。 ①Add the stock solution of the above-mentioned mother flora to the sterile liquid yeast extract medium at a volume ratio (v/v) of 1:150, culture at 40°C, and shake at 180rpm for 24 hours.
②将干稻草秸秆剪成约3cm长,把剪短的干稻草与水按质量/体积比(g/ml)10:4混合,振荡混匀,121℃,20min灭菌,即得浸湿稻草。 ②Cut the dry rice straw into about 3cm long, mix the cut dry rice straw with water at a mass/volume ratio (g/ml) of 10:4, shake and mix well, sterilize at 121°C for 20 minutes, and then get soaked straw .
③取上述①步骤的培养液按体积比(v/v)1: 400加入无菌水中,摇匀后按体积比(v/v)1:10加入到灭菌后的上述②步骤的浸湿稻草中,振荡搅拌均匀,50℃培养7天。即获得兼性厌氧菌群种子发酵剂。 ③ Take the culture solution of the above step ① and add it to the sterile water according to the volume ratio (v/v) 1:400, shake well and add it to the soaking solution of the above ② step after sterilization according to the volume ratio (v/v) 1:10. In straw, shake and stir evenly, and cultivate at 50°C for 7 days. That is to obtain the facultative anaerobic flora seed starter.
二、农业有机废弃物原料按以下百分比进行组合: 2. The raw materials of agricultural organic waste are combined according to the following percentages:
玉米秸杆50%和牛粪50%。 Corn straw 50% and cow dung 50%.
三、堆肥操作过程如下:3. The composting process is as follows:
1. 测量地面面积,至少保证45立方的废弃物原料堆高1.5米以上。初步设定地面面积为15平方米,在其上面铺一层薄膜,然后在薄膜上撒一薄层菌群种子发酵剂。 1. Measure the ground area to ensure that at least 45 cubic meters of waste materials are stacked at a height of more than 1.5 meters. Preliminarily set the ground area to be 15 square meters, spread a layer of film on it, and then sprinkle a thin layer of flora seed starter on the film.
2. 在菌群种子发酵剂上铺厚约30cm的废弃物原料,并加水充分拌匀,使含水量为60%,然后在其上撒一层菌群种子发酵剂。以此类推,直到原料堆高1.5米以上。 2. Spread waste materials about 30cm thick on the flora seed starter, add water and mix well to make the water content 60%, and then sprinkle a layer of flora seed starter on it. And so on, until the raw material pile is more than 1.5 meters high.
3. 在原料堆上覆盖一层雨布并压实,用温度计测定东西南北4个方位的温度并作记录。 3. Cover the raw material pile with a layer of rain cloth and compact it. Use a thermometer to measure the temperature in four directions, east, west, south, north, and make records.
4. 直到堆肥50天后掀开雨布,通过混匀即堆肥过程产生的发酵农业有机肥。 4. Open the rain cloth until 50 days after composting, and mix the fermented agricultural organic fertilizer produced during the composting process.
四、上述的一种多效生物药肥制备见发明专利(ZL201210108437.8)。4. See the invention patent (ZL201210108437.8) for the preparation of the above-mentioned multi-effect biomedical fertilizer.
五、制备功能微生物有机肥:5. Preparation of functional microbial organic fertilizer:
将上述堆肥产生的发酵农业有机肥与一种多效生物药肥混合,混合时按质量百分比计,发酵微生物有机肥70%,多效生物药肥30%。将得到的混合物即多功能微生物有机肥,按50公斤塑料包装袋包装。 The fermented agricultural organic fertilizer produced by the above-mentioned compost is mixed with a multi-effect biological medicinal fertilizer. When mixing, the fermented microbial organic fertilizer is 70% and the multi-effect biological medicinal fertilizer is 30%. The mixture that obtains is the multifunctional microbial organic fertilizer, and is packed in 50 kilograms of plastic bags.
在甜瓜、甜菜和黄瓜土壤中施用本案例产品后可以发现,苗期会促进幼苗的生长发育,使幼苗根茎粗壮,叶色浓绿,生长茁壮,株高明显高于普通有机肥,抗病虫害能力强,特别对黄瓜枯萎病有一定防治作用。结果期果实多,体积大,甜度高,口感好,明显改善成果品质。 After applying the product of this case in the soil of melon, beet and cucumber, it can be found that the seedling stage will promote the growth and development of the seedlings, making the seedlings thick and thick, with dark green leaves, strong growth, plant height significantly higher than ordinary organic fertilizers, and ability to resist diseases and insect pests Strong, especially for the control of cucumber wilt. During the fruiting period, there are many fruits, large volume, high sweetness, and good taste, which obviously improves the fruit quality.
实施例2Example 2
这种多功能微生物有机肥由发酵农业有机肥和中国专利ZL201210108437.8提供的多效生物药肥混合形成,上述两种组份按质量百分比计,发酵农业有机肥80%,多效生物药肥20%;发酵农业有机肥是使用兼性厌氧菌群种子发酵剂与农业有机废弃物原料混合后利用堆肥厌氧发酵方式产生的有机肥,农业有机废弃物原料由稻草秸杆和牛粪混合形成。 This multifunctional microbial organic fertilizer is formed by mixing fermented agricultural organic fertilizer and multi-effect biomedical fertilizer provided by Chinese patent ZL201210108437.8. 20%; Fermented agricultural organic fertilizer is an organic fertilizer produced by using facultative anaerobic flora seed starter mixed with agricultural organic waste raw materials and using compost anaerobic fermentation. The agricultural organic waste raw materials are formed by mixing rice straw and cow dung .
一、兼性厌氧菌群种子发酵剂的制备1. Preparation of facultative anaerobic flora seed starter
1)母菌群制备 1) Preparation of mother flora
将放线菌, 酵母菌, 链霉菌分别接种于固体酵母浸膏(LB)培养基上,在25℃温度下,培养1-3天,用无菌芽签分别挑取单菌落放线菌, 酵母菌, 链霉菌, 然后分别接种到酵母浸膏(LB)液体培养基三角瓶中,180转/分,发酵温度25℃,发酵时间为3天, 然后按比例为放线菌: 酵母菌: 链霉菌=3: 3: 4进行混合,混合液为母菌群; Inoculate Actinomycetes, Yeast, and Streptomyces on solid yeast extract (LB) medium, culture at 25°C for 1-3 days, and pick single colonies of Actinomycetes with sterile bud sticks. Saccharomyces, Streptomyces, and then respectively inoculated into yeast extract (LB) liquid medium Erlenmeyer flask, 180 rpm, fermentation temperature 25°C, fermentation time is 3 days, and then actinomycetes in proportion: Yeast: Streptomyces=3: 3: 4 is mixed, and mixed solution is parent flora;
2)堆肥兼性厌氧菌群种子发酵剂的制备 2) Preparation of compost facultative anaerobic flora seed starter
①将上述的母菌群原液按体积比(v/v)1:150加入到无菌液体酵母浸膏培养基,培养温度40℃,180rpm振荡培养24h。 ①Add the stock solution of the above-mentioned mother flora to the sterile liquid yeast extract medium at a volume ratio (v/v) of 1:150, culture at 40°C, and shake at 180rpm for 24 hours.
②将干稻草秸秆剪成约3cm长,把剪短的干稻草与水按质量/体积比(g/ml)10:4混合,振荡混匀,121℃,20min灭菌,即得浸湿稻草。 ②Cut the dry rice straw into about 3cm long, mix the cut dry rice straw with water at a mass/volume ratio (g/ml) of 10:4, shake and mix well, sterilize at 121°C for 20 minutes, and then get soaked straw .
③取上述①步骤的培养液按体积比(v/v)1: 400加入无菌水中,摇匀后按体积比(v/v)1:10加入到灭菌后的上述②步骤的浸湿稻草中,振荡搅拌均匀,50℃培养7天。即获得兼性厌氧菌群种子发酵剂。 ③ Take the culture solution of the above step ① and add it to the sterile water according to the volume ratio (v/v) 1:400, shake well and add it to the soaking solution of the above ② step after sterilization according to the volume ratio (v/v) 1:10. In straw, shake and stir evenly, and cultivate at 50°C for 7 days. That is to obtain the facultative anaerobic flora seed starter.
二、农业有机废弃物原料按以下百分比进行组合:2. The raw materials of agricultural organic waste are combined according to the following percentages:
稻草秸杆50%和牛粪50%。 Rice straw 50% and cow dung 50%.
三、堆肥操作过程如下:3. The composting process is as follows:
1. 测量地面面积,至少保证45立方的废弃物原料堆高1.5米以上。初步设定地面面积为15平方米,在其上面铺一层薄膜,然后在薄膜上撒一薄层菌群种子发酵剂。 1. Measure the ground area to ensure that at least 45 cubic meters of waste materials are stacked at a height of more than 1.5 meters. Preliminarily set the ground area to be 15 square meters, spread a layer of film on it, and then sprinkle a thin layer of flora seed starter on the film.
2. 在菌群种子菌群培养基液上铺厚约30cm的废弃物原料,并加水充分拌匀,使含水量为60%,然后在其上撒一层菌群种子发酵剂。以此类推,直到原料堆高1.5米以上。 2. Spread waste materials about 30cm thick on the flora seed flora culture medium, add water and mix well to make the water content 60%, and then sprinkle a layer of flora seed starter on it. And so on, until the raw material pile is more than 1.5 meters high.
3. 在原料堆上覆盖一层雨布并压实,用温度计测定东西南北4个方位的温度并作记录。 3. Cover the raw material pile with a layer of rain cloth and compact it. Use a thermometer to measure the temperature in four directions, east, west, south, north, and make records.
4. 直到堆肥60天后掀开雨布,通过混匀即堆肥过程产生的发酵农业有机肥。 4. After 60 days of composting, the rain cloth is lifted, and the fermented agricultural organic fertilizer produced by mixing is the composting process.
四、上述的一种多效生物药肥制备参考发明专利(ZL201210108437.8)4. Reference invention patent for the preparation of the above-mentioned multi-effect biomedical fertilizer (ZL201210108437.8)
五、制备功能微生物有机肥:5. Preparation of functional microbial organic fertilizer:
将上述堆肥产生的发酵农业有机肥与一种多效生物药肥混合,混合时按质量百分比计,发酵有机肥80%,多效生物药肥20%。将得到的混合物即多功能微生物有机肥,按50公斤塑料包装袋包装。 The fermented agricultural organic fertilizer produced by the above-mentioned compost is mixed with a kind of multi-effect biological medicinal fertilizer. When mixing, the fermented organic fertilizer is 80% and the multi-effect biological medicinal fertilizer is 20%. The mixture that obtains is the multifunctional microbial organic fertilizer, and is packed in 50 kilograms of plastic bags.
桃树种植园中本案例多功能微生物有机肥后,枝繁叶茂,开花数量明显增多,花色鲜艳。水蜜桃挂果数量多,病害发生少,甜度高,色泽鲜艳,品质好;未使用本产品的桃树,开花较少,叶子也不浓绿,桃树结实量较少,并且发生严重的桃缩叶病,致使叶片卷曲,产量及品质均下降。 In the peach tree plantation, after the multifunctional microbial organic fertilizer in this case, the branches and leaves are luxuriant, the number of flowers has increased significantly, and the flowers are bright in color. The peaches have a large number of fruiting, few diseases, high sweetness, bright color and good quality; the peach trees without this product have less flowering, less green leaves, less fruiting, and serious peach blossoms Leaf shrinkage, resulting in curly leaves, reduced yield and quality.
实施例3Example 3
这种多功能微生物有机肥由发酵农业有机肥和中国专利ZL201210108437.8提供的多效生物药肥混合形成,上述两种组份按质量百分比计,发酵农业有机肥90%,多效生物药肥10%;发酵农业有机肥是使用兼性厌氧菌群种子发酵剂与农业有机废弃物原料混合后利用堆肥厌氧发酵方式产生的有机肥,农业有机废弃物原料由稻草秸杆、杂草、牛粪混合形成。 This multifunctional microbial organic fertilizer is formed by mixing fermented agricultural organic fertilizer and multi-effect biomedical fertilizer provided by Chinese patent ZL201210108437.8. 10%; fermented agricultural organic fertilizer is an organic fertilizer produced by mixing the seed starter of facultative anaerobic flora with agricultural organic waste raw materials and using compost anaerobic fermentation. The raw materials of agricultural organic waste are rice straw, weeds, Cow manure mixed to form.
一、兼性厌氧菌群种子发酵剂的制备1. Preparation of facultative anaerobic flora seed starter
1)母菌群制备 1) Preparation of mother flora
将放线菌, 酵母菌, 链霉菌分别接种于固体酵母浸膏(LB)培养基上,在25℃温度下,培养1-3天,用无菌芽签分别挑取单菌落放线菌, 酵母菌, 链霉菌, 然后分别接种到酵母浸膏(LB)液体培养基三角瓶中,180转/分,发酵温度25℃,发酵时间为3天, 然后按比例为放线菌: 酵母菌: 链霉菌C=3: 3: 4进行混合,混合液为母菌群; Inoculate Actinomycetes, Yeast, and Streptomyces on solid yeast extract (LB) medium, culture at 25°C for 1-3 days, and pick single colonies of Actinomycetes with sterile bud sticks. Saccharomyces, Streptomyces, and then respectively inoculated into yeast extract (LB) liquid medium Erlenmeyer flask, 180 rpm, fermentation temperature 25°C, fermentation time is 3 days, and then actinomycetes in proportion: Yeast: Streptomyces C=3: 3: 4 is mixed, and mixed solution is parent flora;
2)堆肥中兼性厌氧菌群种子发酵剂的制备 2) Preparation of facultative anaerobic flora seed starter in compost
①将上述的母菌群原液按体积比(v/v)1:150加入到无菌液体酵母浸膏培养基,培养温度40℃,180rpm振荡培养24h。 ①Add the stock solution of the above-mentioned mother flora to the sterile liquid yeast extract medium at a volume ratio (v/v) of 1:150, culture at 40°C, and shake at 180rpm for 24 hours.
②将干稻草秸秆剪成约3cm长,把剪短的干稻草与水按质量/体积比(g/ml)10:4混合,振荡混匀,121℃,20min灭菌,即得浸湿稻草。 ②Cut the dry rice straw into about 3cm long, mix the cut dry rice straw with water at a mass/volume ratio (g/ml) of 10:4, shake and mix well, sterilize at 121°C for 20 minutes, and then get soaked straw .
③取上述①步骤的培养液按体积比(v/v)1: 400加入无菌水中,摇匀后按体积比(v/v)1:10加入到灭菌后的上述②步骤的浸湿稻草中,振荡搅拌均匀,50℃培养7天。即获得兼性厌氧菌群种子发酵剂。 ③ Take the culture solution of the above step ① and add it to the sterile water according to the volume ratio (v/v) 1:400, shake well and add it to the soaking solution of the above ② step after sterilization according to the volume ratio (v/v) 1:10. In straw, shake and stir evenly, and cultivate at 50°C for 7 days. That is to obtain the facultative anaerobic flora seed starter.
二、农业有机废弃物原料按以下百分比进行组合: 2. The raw materials of agricultural organic waste are combined according to the following percentages:
稻草秸杆70,杂草10%和牛粪20%。 Rice Straw 70, Weeds 10% and Cow Dung 20%.
三、堆肥操作过程如下:3. The composting process is as follows:
1. 测量地面面积,至少保证45立方的废弃物原料堆高1.5米以上。初步设定地面面积为15平方米,在其上面铺一层薄膜,然后在薄膜上撒一薄层菌群种子发酵剂。 1. Measure the ground area to ensure that at least 45 cubic meters of waste materials are stacked at a height of more than 1.5 meters. Preliminarily set the ground area to be 15 square meters, spread a layer of film on it, and then sprinkle a thin layer of flora seed starter on the film.
2. 在菌群种子发酵剂上铺厚约30cm的废弃物原料,并加水充分拌匀,使含水量为60%,然后在其上撒一层菌群种子发酵剂。以此类推,直到原料堆高1.5米以上。 2. Spread waste materials about 30cm thick on the flora seed starter, add water and mix well to make the water content 60%, and then sprinkle a layer of flora seed starter on it. And so on, until the raw material pile is more than 1.5 meters high.
3. 在原料堆上覆盖一层雨布并压实,用温度计测定东西南北4个方位的温度并作记录。 3. Cover the raw material pile with a layer of rain cloth and compact it. Use a thermometer to measure the temperature in four directions, east, west, south, north, and make records.
4. 直到堆肥60天后掀开雨布,通过混匀即堆肥过程产生的发酵农业有机肥。 4. After 60 days of composting, the rain cloth is lifted, and the fermented agricultural organic fertilizer produced by mixing is the composting process.
四、上述的一种多效生物药肥制备见发明专利(ZL201210108437.8)4. See the invention patent (ZL201210108437.8) for the preparation of the above-mentioned multi-effect biological fertilizer
五、制备功能微生物有机肥:5. Preparation of functional microbial organic fertilizer:
将上述堆肥产生的发酵农业有机肥与一种多效生物药肥混合,混合时按质量百分比计,发酵有机肥90%,多效生物药肥10%。将得到的混合物即多功能微生物有机肥,按50公斤塑料包装袋包装。 The fermented agricultural organic fertilizer produced by the above-mentioned compost is mixed with a multi-effect biomedical fertilizer. When mixing, the fermented organic fertilizer is 90% and the multi-effect biomedical fertilizer is 10%. The mixture that obtains is the multifunctional microbial organic fertilizer, and is packed in 50 kilograms of plastic bags.
在草坪种植区使用本案例产品后,草坪草侧根多,根系生长茂盛;地上部分叶片数量多,叶色浓密,颜色鲜绿,抗踩踏。 After using the product in this case in the lawn planting area, the turfgrass has many lateral roots and the root system grows lushly; the number of leaves on the ground part is large, the leaves are dense, bright green in color, and resistant to trampling.
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