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    BIOMARK Laboratories-INDIA: Technical Sheet

    Uploaded by

    Jose Suarez
    This technical sheet provides information about Thiobacillus Broth (B775), which is used for cultivating Thiobacillus species. The broth contains ammonium sulphate, monopotassium phosphate, calcium chloride, ferrous sulphate, magnesium sulphate, and sodium thiosulphate. To prepare, 10.16g of the powder is suspended in 1000ml of distilled water, boiled to dissolve, and autoclaved. Thiobacillus species are identified by turbidity or sulphur precipitation within 7 days of incubating samples in the broth at 25-30°C. Quality control tests confirm the medium supports growth of Thiobacillus thiooxidans

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    BIOMARK Laboratories-INDIA: Technical Sheet

    Uploaded by

    Jose Suarez
    110 views1 page
    This technical sheet provides information about Thiobacillus Broth (B775), which is used for cultivating Thiobacillus species. The broth contains ammonium sulphate, monopotassium phosphate, calcium chloride, ferrous sulphate, magnesium sulphate, and sodium thiosulphate. To prepare, 10.16g of the powder is suspended in 1000ml of distilled water, boiled to dissolve, and autoclaved. Thiobacillus species are identified by turbidity or sulphur precipitation within 7 days of incubating samples in the broth at 25-30°C. Quality control tests confirm the medium supports growth of Thiobacillus thiooxidans

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    This technical sheet provides information about Thiobacillus Broth (B775), which is used for cultivating Thiobacillus species. The broth contains ammonium sulphate, monopotassium phosphate, calcium chloride, ferrous sulphate, magnesium sulphate, and sodium thiosulphate. To prepare, 10.16g of the powder is suspended in 1000ml of distilled water, boiled to dissolve, and autoclaved. Thiobacillus species are identified by turbidity or sulphur precipitation within 7 days of incubating samples in the broth at 25-30°C. Quality control tests confirm the medium supports growth of Thiobacillus thiooxidans

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    110 views1 page

    BIOMARK Laboratories-INDIA: Technical Sheet

    Uploaded by

    Jose Suarez
    This technical sheet provides information about Thiobacillus Broth (B775), which is used for cultivating Thiobacillus species. The broth contains ammonium sulphate, monopotassium phosphate, calcium chloride, ferrous sulphate, magnesium sulphate, and sodium thiosulphate. To prepare, 10.16g of the powder is suspended in 1000ml of distilled water, boiled to dissolve, and autoclaved. Thiobacillus species are identified by turbidity or sulphur precipitation within 7 days of incubating samples in the broth at 25-30°C. Quality control tests confirm the medium supports growth of Thiobacillus thiooxidans

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
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    BIOMARK Laboratories-INDIA

    www.biomarklabs.com
    TECHNICAL SHEET

    B775 THIOBACILLUS BROTH


    Formula
    Ingredients : gms/lit.
    Ammonium sulphate 0.40
    Monopotassium phosphate 4.00
    Calcium chloride 0.25
    Ferrous sulphate 0.01
    Magnesium sulphate 0.50
    Sodium thiosulphate 5.00

    Final pH (at 25°C) : Self


    Directions :
    Suspend 10.16 gms in 1000 ml distilled water. Boil to dissolve the medium completely. Sterilize
    by autoclaving at 15 lbs pressure (121°C) for 15 minutes & dispense as desired.
    Principle :
    Samples are inoculated into Thiobacillus Broth, After incubation at 25-30˚C for about 7 days or
    more,turbidity or sulphur precipitation on the surface of the liquid or against the walls of the
    flasks, indicates growth of bacteria.Isolation is subsequently done on Thiobacillus
    Agar.Thiobacillus forms small sulphur impregnated colonies with clear zones, indicating &
    formation from thiosulphate oxidation.
    QC Tests – (I)Dehydrated Medium
    Colour : Off white
    Appearance : Homogeneous Free Flowing powder
    (II)Rehydrated medium
    pH (post autoclaving/heating) : Self
    Colour (post autoclaving/heating) : Light amber
    Clarity (post autoclaving/heating) : Clear to slightly opalescent
    (III)Q.C. Test Microbiological
    Cultural characteristics observed after 7 days at 25 - 30°C.
    MICROORGANISM (ATCC ) GROWTH
    Thiobacillus thiooxidans Good – luxuriant
    Thiobacillus thioparus Good – luxuriant

    Precautions : 1. For Laboratory Use.


    2. Follow proper, established laboratory procedures in handling and disposing of
    infectious materials.
    Limitations : 1. Since the nutritional requirements of organisms vary, some strains may be
    encountered that fail to grow or grow poorly on this medium.
    Use : B775: For cultivation of Thiobacillus species.
    Storage : Dehydrated medium- below 30°C Prepared medium– Between 2 to 8°C.
    Packing : 500 gm. bottle
    Product profile: Reconstitution Quantity on pH (25°C) Supplement Sterilization
    Preparation
    (500g)
    B775 10.16 g/l 49.21L Self NIL 1210C /15 min.

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