Dey-Engley Neutralizing Broth

Dey-Engley Neutralizing Broth M1062
Dey-Engley Neutralizing Broth is used in disinfectant testing where neutralization of the antiseptics and disinfectants is
important for determining its bactericidal activity.
Composition**
Ingredients Gms / Litre
Casein enzymic hydrolysate 5.000
Yeast extract 2.500
Dextrose 10.000
Sodium thioglycollate 1.000
Sodium thiosulphate 6.000
Sodium bisulphite 2.500
Lecithin 7.000
Polysorbate 80 5.000
Bromocresol purple 0.020
Final pH ( at 25°C) 7.6±0.2
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 39.02 grams in 1000 ml distilled water. Heat if necessary to dissolve the medium completely. Sterilize by autoclaving
at 15 lbs pressure (121°C) for 15 minutes. Mix well and dispense as desired.
Principle And Interpretation

Dey-Engley Neutralizing Broth is formulated as per the procedure described by Engley and Dey (1). Dey -Engley Neutralizing
Broth is especially suited for environmental sampling where neutralization of the chemical is important to determine its
bactericidal activity. A strongly bacteriostatic substance inhibits the growth and reproduction of bacteria without killing them.
These bacteria hold the ability to cause infection under favourable conditions. Dey-Engley Neutralizing Broth Base and Dey-
Engley Neutralizing Broth has the same formula but the former does not containing the neutralizing components.
The Dey-Engley Neutralizing Broth neutralizes a broad spectrum of antiseptics and disinfectants including quaternary
ammonium compounds, phenolics, iodine and chlorine preparations, mercurials, formaldehyde and glutaraldehyde. Dey-
Engley Neutralizing Broth is used for the neutralization and testing of antiseptics and disinfectants according to the procedure
of Engley and Dey (1).
Casein enzymic hydrolysate provides essential nutrients. Dextrose is an energy source. Dextrose is an energy source. Yeast
extract is also a rich source of vitamin B-complex. The present formulation incorporate neutralizing substances for almost all the
active products used as antiseptics and disinfectants. Sodium bisulfite neutralizes aldehydes; sodium thioglycollate neutralizes
mercurials; sodium thiosulfate neutralizes iodine and chlorine (1); lecithin neutralizes quaternary ammonium compounds; and
polysorbate 80, a non-ionic surface-active agent, neutralizes substituted phenolics (2-5). Bromocresol purple is an indicator for
dextrose utilization. Due to the high concentration of lecithin in the broth medium, turbidity cannot be used to detect growth.
Therefore, bromocresol purple and dextrose are added to the medium. Those organisms that ferment dextrose will turn the
medium from purple to yellow. Growth of Pseudomonas species, which do not ferment dextrose, can be detected by the
formation of a pellicle on the surface of the bro\th (1).
Neutralization Test
For testing disinfectants, prepare two sets of test tubes, one containing 9 ml Dey-Engley Neutralizing Broth (M1062) and other
with 9 ml Dey-Engley Neutralizing Broth Base (M187). Add 1 ml of disinfectant under test. Mix well and allow it to stand
for 15 minutes. Inoculate 0.1 ml of 1:100,000 dilution of overnight broth cultures and incubate at 37°C for 48 hours. Growth
is indicated by a colour change from purple to yellow or pellicle formation. Growth in Neutralizing Broth and no growth in
Please refer disclaimer Overleaf.

HiMedia Laboratories Technical Data
Neutralizing Broth Base indicate neutralization of disinfectant. To check bactericidal activity, both broth tubes are inoculated
on D/E Neutralizing Agar (M186). Positive growth from negative tubes of Neutralizing Broth Base indicates bacteriostatic
substance while negative growth indicates a bactericidal disinfectant. All positive tubes should show growth on Dey-Engley
Neutralizing Agar. The control disinfectants used in test procedure are 2% chlorine, 2% formaldehyde, 1% glutaraldehyde, 2%
iodine, 2% phenol, 1/750 quaternary ammonium compounds, 1/1000 mercurials etc.
Quality Control
Appearance
Light yellow to bluish grey homogeneous free flowing powder
Colour and Clarity of prepared medium
Purple coloured, opalescent solution in tubes
Reaction
Reaction of 3.9% w/v aqueous solution at 25°C. pH : 7.6±0.2
pH
7.40-7.80
Cultural Response
M1062: Cultural characteristics observed after an incubation at 35-37°C for 40-48 hours.
Organism Inoculum Growth
(CFU)
Cultural Response
Bacillus subtilis ATCC 6633 50-100 luxuriant
Escherichia coli ATCC 50-100 luxuriant
25922
Pseudomonas aeruginosa 50-100 luxuriant
ATCC 27853
Salmonella Typhimurium 50-100 luxuriant
ATCC 14028
Staphylococcus aureus 50-100 luxuriant
ATCC 25923
Escherichia coli ATCC 8739 50-100 luxuriant
Staphylococcus aureus 50-100 luxuriant
ATCC 6538
Storage and Shelf Life

Store below 30°C in tightly closed container and use freshly prepared medium. Use before expiry date on the label.
Reference
1. Engley and Dey, 1970. Chem. Spec. Manuf. Assoc. Proc., Mid-Year Meet., p. 100.
Revision : 2 / 2015
Disclaimer :
User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained in
this and other related HiMedia™ publications. The information contained in this publication is based on our research and development
work and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes to
specifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use but
for laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not
be considered as a warranty of any kind, expressed or implied, and no liability is accepted for infringement of any patents.
HiMedia Laboratories Pvt. Ltd. A-516,Swastik Disha Business Park,Via Vadhani Ind. Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-6147
1919 Email: techhelp@himedialabs.com

Dey-Engley Neutralizing Broth

Uploaded by

Copyright:

Available Formats

Dey-Engley Neutralizing Broth

Uploaded by

Document Information

Original Description:

Copyright

Available Formats

Share this document

Share or Embed Document

Sharing Options

Did you find this document useful?

Is this content inappropriate?

Copyright:

Available Formats

Dey-Engley Neutralizing Broth

Uploaded by

Copyright:

Available Formats

Dey-Engley Neutralizing Broth M1062

Principle And Interpretation

Please refer disclaimer Overleaf.

Storage and Shelf Life

You might also like