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    M1245

    Uploaded by

    Chairil Anwar
    BERTANI

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd

    M1245

    Uploaded by

    Chairil Anwar
    77 views2 pages
    BERTANI

    Copyright

    © © All Rights Reserved

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    PDF, TXT or read online from Scribd

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    BERTANI

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    77 views2 pages

    M1245

    Uploaded by

    Chairil Anwar
    BERTANI

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    You are on page 1of 2

    Luria Bertani Broth, Miller M1245

    Luria Bertani Broth, Miller is used for the cultivation and maintenance of recombinant strains of Escherichia coli and may
    be used for routine cultivation of not particularly fastidious microorganisms.
    Composition**
    Ingredients Gms / Litre
    Casein enzymic hydrolysate 10.000
    Yeast extract 5.000
    Sodium chloride 10.000
    Final pH ( at 25C) 7.50.2
    **Formula adjusted, standardized to suit performance parameters

    Directions
    Suspend 25 grams in 1000 ml distilled water. Heat if necessary to dissolve the medium completely. Sterilize by autoclaving
    at 15 lbs pressure (121C) for 15 minutes. Dispense as desired.

    Principle And Interpretation


    Luria Bertani Broth, Miller (1) is slightly different with double amount of sodium chloride as compared to original media
    described by Lennox (2) for cultivation and maintenance of recombinant strains of Escherichia coli . The media is
    nutritionally rich for the growth of pure cultures of recombinant strains. Strains derived from Escherichia coli K12 are
    deficient in Vitamin B synthesis which are further modified by specific mutation to create auxotrophic strains and are therefore
    unable to grow on nutritionally deficient media.
    Casein enzymic hydrolysate provides peptides and peptones while Vitamin B complex is provided by yeast extract. Sodium
    chloride provides sodium ions for membrane transport and also maintains the osmotic equilibrium of the medium.

    Quality Control
    Appearance
    Cream to yellow homogeneous free flowing powder
    Colour and Clarity of prepared medium
    Yellow to amber coloured clear solution in tubes
    Reaction
    Reaction of 2.5% w/v aqueous solution at 25C. pH : 7.50.2
    pH
    7.30-7.70
    Cultural Response
    M1245: Cultural characteristics observed after an incubation at 35 - 37C for 18 - 24 hours.
    Organism Inoculum Growth
    (CFU)
    Cultural Response
    Escherichia coli ATCC 50-100 luxuriant
    23724
    Escherichia coli ATCC 50-100 luxuriant
    25922
    Escherichia coli DH5 alpha 50-100 luxuriant
    MTCC 1652

    Storage and Shelf Life


    Store below 30C in tightly closed container and the prepared medium at 2 - 8C. Use before expiry date on the label.

    Reference

    Please refer disclaimer Overleaf.


    HiMedia Laboratories Technical Data

    1. Lennox E.S./ 1955, Transduction of Linked Genetic Characters of the host by bacteriophage P1., Virology, 1:190.
    2. Atlas R.M., 1983, Handbook of Microbiological Media, Ed. By Parks L., CRC Press, Inc.

    Revision : 2 / 2015

    Disclaimer :
    User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained in
    this and other related HiMedia publications. The information contained in this publication is based on our research and development
    work and is to the best of our knowledge true and accurate. HiMedia Laboratories Pvt Ltd reserves the right to make changes to
    specifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use but
    for laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not
    be considered as a warranty of any kind, expressed or implied, and no liability is accepted for infringement of any patents.

    HiMedia Laboratories Pvt. Ltd. A-516,Swastik Disha Business Park,Via Vadhani Ind. Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-6147
    1919 Email: techhelp@himedialabs.com

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