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    Yeast Malt Broth: Composition

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    Sumit
    Yeast Malt Broth is a growth medium used for isolating and culturing yeasts, molds, and other acid-tolerant microorganisms. It contains peptic digest of animal tissue, yeast extract, malt extract, and dextrose to support growth, and can be acidified to increase selectivity. The document provides instructions for preparation and use of the medium, as well as results from quality control testing.

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    Yeast Malt Broth: Composition

    Uploaded by

    Sumit
    72 views2 pages
    Yeast Malt Broth is a growth medium used for isolating and culturing yeasts, molds, and other acid-tolerant microorganisms. It contains peptic digest of animal tissue, yeast extract, malt extract, and dextrose to support growth, and can be acidified to increase selectivity. The document provides instructions for preparation and use of the medium, as well as results from quality control testing.

    Original Description:

    Yeast media broth culture

    Original Title

    YM broth

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    Yeast Malt Broth is a growth medium used for isolating and culturing yeasts, molds, and other acid-tolerant microorganisms. It contains peptic digest of animal tissue, yeast extract, malt extract, and dextrose to support growth, and can be acidified to increase selectivity. The document provides instructions for preparation and use of the medium, as well as results from quality control testing.

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    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    72 views2 pages

    Yeast Malt Broth: Composition

    Uploaded by

    Sumit
    Yeast Malt Broth is a growth medium used for isolating and culturing yeasts, molds, and other acid-tolerant microorganisms. It contains peptic digest of animal tissue, yeast extract, malt extract, and dextrose to support growth, and can be acidified to increase selectivity. The document provides instructions for preparation and use of the medium, as well as results from quality control testing.

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    © All Rights Reserved
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    Yeast Malt Broth M425

    Yeast Malt Broth (YM Broth) is used for the isolation and cultivation of yeasts, moulds and other aciduric microorganisms.
    Composition**
    Ingredients Gms / Litre
    Peptic digest of animal tissue 5.000
    Yeast extract 3.000
    Malt extract 3.000
    Dextrose 10.000
    Final pH ( at 25°C) 6.2±0.2
    **Formula adjusted, standardized to suit performance parameters

    Directions
    Suspend 10.5 grams in 490 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving
    at 15 lbs pressure (121°C) for 15 minutes. For preparing selective media acidify the media upto pH 3.0 to 4.0 by aseptically
    adding 1 vial of 10% Lactic Acid solution (FD095) or add antibiotics. DO NOT HEAT the media after addition of acid or
    antibiotics. Mix well and dispense as desired.

    Principle And Interpretation


    Yeast Malt Broth is formulated as per Wickerham (1, 2) for isolation and cultivation of yeasts, moulds and other aciduric
    microorganisms. Fungistatic materials such as sodium propionate and diphenyl are added to YM Broth to eliminate moulds
    and thus permits enumeration of yeasts from mixed population.
    Wickerham suggested the use of Yeast Malt Broth as an enrichment medium for yeasts by adding a layer of sterile paraffin oil
    (about 1 cm) on the surface of inoculated broth. After the growth occurs it should be streaked on YM Agar (M424) to obtain
    isolated colonies of fermentative species. To isolate fermentative as well as oxidative strains, acidified YM Broth is placed
    on a rotary shaker for 1 or 2 days which favours development of yeast cells while the sporulation of moulds is prevented and
    yeasts can be isolated by streaking on YM Agar (M424).
    Peptic digest of animal tissue serves as a source of carbon, nitrogen and essential nutrients. Yeast extract supplies vitamin B
    complex nutrients and other growth factors. Malt extract serves as an additional source of carbon. Dextrose is the carbohydrate
    and energy source. To increase the selectivity, the media can be acidified by the addition of sterile 10% HCl, tartaric acid
    or 10% citric acid. Alternatively antibiotics (penicillin 20U/ml or streptomycin to a final concentration of 40mcg/ml) can be
    added. Acidified medium should not be reheated.

    Quality Control
    Appearance
    Cream to beige homogeneous free flowing powder
    Colour and Clarity of prepared medium
    Light amber coloured clear solution in tubes.
    pH
    6.00-6.40
    Cultural Response
    M425: Cultural characteristics observed after an incubation at 25-30°C for 40-72 hours.
    Organism Growth at pH Growth at pH
    3.4 6.2
    Cultural Response
    *Aspergillus brasiliensis good-luxuriant good-luxuriant
    ATCC 16404

    Please refer disclaimer Overleaf.


    HiMedia Laboratories Technical Data

    Candida albicans ATCC good-luxuriant good-luxuriant


    10231
    Escherichia coli ATCC inhibited good-luxuriant
    25922
    Lactobacillus leichmannii poor good-luxuriant
    ATCC 4797
    Saccharomyces cerevisiae good-luxuriant good-luxuriant
    ATCC 9763
    Lactobacillus casei ATCC poor good-luxuriant
    9595

    Storage and Shelf Life


    Store below 30°C in tightly closed container and the prepared medium at 2-8°C. Use before expiry date on the label.

    Reference
    1. Wickerham, 1939, J. Tropical Med. Hyg., 42:176.
    2. Wickerham, 1951, U.S. Dept. Agric. Tech. Bull. No. 1029.

    Revision : 1 / 2011

    Disclaimer :
    User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained in
    this and other related HiMedia™ publications. The information contained in this publication is based on our research and development
    work and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes to
    specifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use but
    for laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not
    be considered as a warranty of any kind, expressed or implied, and no liability is accepted for infringement of any patents.

    HiMedia Laboratories Pvt. Ltd. A-516,Swastik Disha Business Park,Via Vadhani Ind. Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-6147
    1919 Email: techhelp@himedialabs.com

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