Vetch associated disease (hairy vetch poisoning) was observed in 8 herds of dairy cows in the state of Rio Grande do Sul, southern Brazil. In the pasture where 4 of these 8 herds were, Vicia villosa was the only vetch species represented,... more
Vetch associated disease (hairy vetch poisoning) was observed in 8 herds of dairy cows in the state of Rio Grande do Sul, southern Brazil. In the pasture where 4 of these 8 herds were, Vicia villosa was the only vetch species represented, while cattle in the remaining 4 herds had access to both V villosa and V sativa but with large predominance of the former. Observed clinical signs included fever, dramatic drop in milk yield, thickening and wrinkling of the skin with multifocal plaques of alopecia, pruritus, conjunctivitis, nasal and ocular serous discharge, loss of weight and diarrhea. The mean morbidity in the 8 affected herds, representing 219 cattle, was 11.1% and the mortality was 100%. The duration of the clinical disease varied from 10 to 30 d. Gross lesions consisted of multifocal to coalescing grey-white soft to moderately firm nodules which infiltrated several organs, but were particularly prominent in lymph nodes, adrenal, renal cortex, spleen, liver, and myocardium. Mic...
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Pythium insidiosum iron acquisition mechanisms are unknown. We previously showed that the iron chelator deferasirox had weak activity in vitro and in rabbits with experimental pythiosis. Here we show that deferasirox causes damage to P.... more
Pythium insidiosum iron acquisition mechanisms are unknown. We previously showed that the iron chelator deferasirox had weak activity in vitro and in rabbits with experimental pythiosis. Here we show that deferasirox causes damage to P. insidiosum hyphae in vitro, but that activity is diminished in the presence of exogenous iron. The tissue activity of the proinflammatory enzyme adenosine deaminase and the histological pattern observed in pythiosis lesions of rabbits treated with deferasirox were similar to the ones in animals treated with immunotherapy.
Research Interests: Multidisciplinary, Pythium, Animals, Immunotherapy, Immunomodulation, and 4 moreIron, PLoS one, Rabbits, and Triazoles
An outbreak of acute seasonal allergic dermatitis is described in sheep from the municipalities of São Vicente do Sul and Jaguari, Rio Grande do Sul, Brazil. Affected sheep were crossbreeds of different categories. Skin lesions were... more
An outbreak of acute seasonal allergic dermatitis is described in sheep from the municipalities of São Vicente do Sul and Jaguari, Rio Grande do Sul, Brazil. Affected sheep were crossbreeds of different categories. Skin lesions were observed mainly in the head (ears and around the eyes, muzzle, and lips), mammary gland, distal portions of the limbs and ventral abdomen. Two
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The biopsy files in dogs from the Veterinary Pathology Laboratory of the Universidade Federal de Santa Maria, RS, Brazil, from 1964 to 2003, were reviewed for skin tumors. In the 703 files, 570 (81.0%) it was reported that the dog had... more
The biopsy files in dogs from the Veterinary Pathology Laboratory of the Universidade Federal de Santa Maria, RS, Brazil, from 1964 to 2003, were reviewed for skin tumors. In the 703 files, 570 (81.0%) it was reported that the dog had only one skin tumor and in 133 (19.0%), more than one tumor was described in the skin. Six hundred
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1 Recebido em 16 de maio de 2008. Aceito para publicação em 5 de junho de 2008. Parte da dissertação de Mestrado do primeiro autor. Financiado inte-gralmente pelo Grant de Pesquisador IA do CNPq (Proc.307876/2006-3). 2 Programa de... more
1 Recebido em 16 de maio de 2008. Aceito para publicação em 5 de junho de 2008. Parte da dissertação de Mestrado do primeiro autor. Financiado inte-gralmente pelo Grant de Pesquisador IA do CNPq (Proc.307876/2006-3). 2 Programa de Pós-Graduação em Medicina ...
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ABSTRACT This experiment was design to address the following points in relation to the acute poisoning by bracken fern (Pteridium aquilinum) in cattle: 1) the severity of the thrombocytopenia in different stages of the intoxication and... more
ABSTRACT This experiment was design to address the following points in relation to the acute poisoning by bracken fern (Pteridium aquilinum) in cattle: 1) the severity of the thrombocytopenia in different stages of the intoxication and its relationship to possible deficits in the secondary hemostasis, 2) the relationship between neutropenia and the morphological signs of septicemia occasionally found at necropsy, and 3) the mechanism of anemia and its relationship with blood loss, medium life of erythrocytes and the progress of the disease. The fresh green upper parts of P. aquilinum were fed to four mixed breed calves with average age of 18 months and weights ranging from 190 to 215 kg. A calf of similar age and weight was kept together with the other four under the same conditions, except for the ingestion of P. aquilinum. The four fern-fed calves died with typical features of acute bracken fern poisoning after being fed with the plant for 53-58 days daily doses of 8.0, 8.6, 10.2, and 10.6g/kg body weight totaling at the end of the experiment, respectively, 59.3%, 63.3%, 47.4%, and 47.5% of bracken fern in relation to their body weight. The disease was characterized by fever up to 42.5°C and varying degrees of hemorrhages observed clinically, at necropsy and on histological examination. Death occurred 6-7 days after the onset of fever. The hematological changes consisted mainly of marked thrombocytopenia and neutropenia. Two of the four affected calves had mild anemia. The deficits in secondary hemostasis were mild in each case. There were no significant changes thus permitting to rule out the role of secondary hemostasis in the pathogenesis of the hemorrhages in the bracken fern poisoning. The measurement of fibrin degradation products in the serum showed conflicting results and did no allow for a solid conclusion regarding the role of disseminated intravascular coagulation in the pathogenesis of the hemorrhages in fern poisoning. The cytology and the histopathology of bone marrow from the four poisoned calves revealed marked decrease in the numbers of hematopoietic cells from the three marrow lineages, which characterizes marrow failure due to aplasia; it was concluded that the events of the primary hemostasis due to thrombocytopenia are responsible for the hemorrhages. In blood culture from three affected calves there was growth of Klebsiella oxytoca, Staphylococcus hyicus and Staphylococcus aureus, indicating that septicemia, facilitated by neutropenia could have a role in the death of cattle acutely poisoned due to the ingestion of P. aquilinum. Additional points of the interest in the acute experimental poisoning by bracken fern in cattle reported here were the development of hematuria and the so called laryngeal form.
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Tecidos de 27 cães que morreram de hepatite infecciosa canina (HIC) foram avaliados por imunoistoquímica (IHQ) para detecção de antígeno de adenovírus canino tipo 1 (CAV-1), por meio de um anticorpo monoclonal. Os tecidos examinados... more
Tecidos de 27 cães que morreram de hepatite infecciosa canina (HIC) foram avaliados por imunoistoquímica (IHQ) para detecção de antígeno de adenovírus canino tipo 1 (CAV-1), por meio de um anticorpo monoclonal. Os tecidos examinados incluíram fígado, rim, baço, linfonodos, tonsilas, ...
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Cryptococcus neoformans, the etiological agent of cryptococcosis, is an opportunistic fungal pathogen of immunocompromised individuals. The aim of this study was to evaluate the activities of E-NTPDase and E-ADA in rats experimentally... more
Cryptococcus neoformans, the etiological agent of cryptococcosis, is an opportunistic fungal pathogen of immunocompromised individuals. The aim of this study was to evaluate the activities of E-NTPDase and E-ADA in rats experimentally infected by C. neoformans var. grubii. Adult rats (35) were divided in two groups: 18 for the control group (uninfected) (A), and 17 for the infected group (B). Each group was separated into three sub-groups (A1, A2, A3-B1, B2, B3), and samples were collected on 10, 20, and 30 days post-infection (PI). Leukocyte counts, IFN-γ, TNF-α, IgM, IgG levels, and E-NTPDase and E-ADA activities were analyzed. It was possible to observe that IgG and IgM seric levels of infected rats were significantly elevated (P<0.01) on days 10, 20 and 30 PI, as well as the levels of TNF-α and INF-γ when compared to uninfected rodents. Regarding E-NTPDase activity in lymphocytes, it was possible to observe that the ATP hydrolysis was significantly decreased on days 20 (P<...
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ABSTRACT. - Barros C.S.L., Fighera R.A., Rozza D.B., Rech R.R., Sallis S.V. & Langohr I.M. 2001. (Systemic granulomatous disease in cattle in Rio Grande do Sul, Brazil, associated with Systemic granulomatous disease in cattle in Rio... more
ABSTRACT. - Barros C.S.L., Fighera R.A., Rozza D.B., Rech R.R., Sallis S.V. & Langohr I.M. 2001. (Systemic granulomatous disease in cattle in Rio Grande do Sul, Brazil, associated with Systemic granulomatous disease in cattle in Rio Grande do Sul, Brazil, associated with grazing vetch ( grazing vetch (Vicia Vicia spp) spp)) Doença granulomatosa sistêmica em bovinos no Rio Grande do
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... Necropsy findings related to the cause of death in 335 horses: 1968-2007. Felipe Pierezan I ; Daniel R. Rissi I ; Raquel R. Rech II ; Rafael A. Fighera III ; Juliana S. Brum IV ; Claudio SL Barros III, *. ... J. 13:43-46. [ Links ].... more
... Necropsy findings related to the cause of death in 335 horses: 1968-2007. Felipe Pierezan I ; Daniel R. Rissi I ; Raquel R. Rech II ; Rafael A. Fighera III ; Juliana S. Brum IV ; Claudio SL Barros III, *. ... J. 13:43-46. [ Links ]. Barros CSL, Barros SS, Santos MN & Souza MA 1984. ...
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The present study was carried out in order to assess the possible alterations in purine levels of brain, associated neuronal lesions in gerbils experimentally infected with Neospora caninum. For that, gerbils (Meriones unguiculatus) were... more
The present study was carried out in order to assess the possible alterations in purine levels of brain, associated neuronal lesions in gerbils experimentally infected with Neospora caninum. For that, gerbils (Meriones unguiculatus) were inoculated with Nc-1 strain of N. caninum, composing two different experiments: Experiment I (EI) and experiment II (EII), where purine levels were measured along with the histopathologic study, on days 7 (EI), 15 and 30 (EII), post-infection (PI). As a result, it was possible to observe that the purine levels (ATP, ADP, AMP, adenosine, inosine and xanthine) in brain in EI are significantly reduced (p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.05), while in EII we faced a different pattern, since in the majority the purine levels were significantly increased (p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.05) on days 15 (ATP, AMP, adenosine, hypoxanthine and xanthine) and 30 PI (ATP, ADP, AMP, adenosine, and uric acid). Results of brain histopathology did not show histological lesion in animals of EI; however, in gerbils of EII it was possible to verify that the alterations (lesions) were more pronounced in gerbils evaluated on day 30 PI when compared to day 15 PI. Therefore, it was possible to conclude that the purine levels in brain were altered in both experiments, concomitant with the histopathological injuries observed in EII.
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... Estudo retrospectivo de 1.647 tumores mamários em cães. José C. Oliveira Filho I ; Glaucia D. Kommers II ; Eduardo K. Masuda I ; Brenda MFPP Marques III ; Rafael A. Fighera II ; Luiz F. Irigoyen II ; Claudio SL Barros II. ... 2001, De... more
... Estudo retrospectivo de 1.647 tumores mamários em cães. José C. Oliveira Filho I ; Glaucia D. Kommers II ; Eduardo K. Masuda I ; Brenda MFPP Marques III ; Rafael A. Fighera II ; Luiz F. Irigoyen II ; Claudio SL Barros II. ... 2001, De Nardi et al. 2002, Martins et al. ...
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... experimental, Documentos relacionados. Id: 522556. Autor: Alda, Joaquín L. de; Sallis, Eliza Simone V; Nogueira, Carlos Eduardo W; Soares, Mauro P; Amaral, Lorena; Marcolongo-Pereira, Clairton; Xavier, Fernanda; Frey Jr, Friedrich;... more
... experimental, Documentos relacionados. Id: 522556. Autor: Alda, Joaquín L. de; Sallis, Eliza Simone V; Nogueira, Carlos Eduardo W; Soares, Mauro P; Amaral, Lorena; Marcolongo-Pereira, Clairton; Xavier, Fernanda; Frey Jr, Friedrich; Schild, Ana Lucia. ...
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... Aline RodriguesI; Rafael A. FigheraI; Tatiana M. SouzaI; Ana Lucia SchildII; Mauro P. SoaresII; Joaquim MilanoIII; Claudio S.L. BarrosIV, * ... In: Kumar V., Abbas AK & Fausto N. (ed.) Robbins and Cotran Pathologic Basis of... more
... Aline RodriguesI; Rafael A. FigheraI; Tatiana M. SouzaI; Ana Lucia SchildII; Mauro P. SoaresII; Joaquim MilanoIII; Claudio S.L. BarrosIV, * ... In: Kumar V., Abbas AK & Fausto N. (ed.) Robbins and Cotran Pathologic Basis of Disease. 7th ed. Elsevier Saunders, Philadelphia. ...
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... Rafael A. Fighera2, Tatiana M. Souza2, Ingeborg Langohr2 e Claudio SL Barros2 ... Os animais que ingerem grande quantidade da planta de uma única vez desenvolvem metemoglobinemia, tornando-se apáticos, hipotérmicos e cianóticos... more
... Rafael A. Fighera2, Tatiana M. Souza2, Ingeborg Langohr2 e Claudio SL Barros2 ... Os animais que ingerem grande quantidade da planta de uma única vez desenvolvem metemoglobinemia, tornando-se apáticos, hipotérmicos e cianóticos (Weiser 1992). ...
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The objective of this study was to evaluate the pathogenesis of ascites in mice infected with Toxoplasma gondii and gerbils infected with Neospora caninum during the acute phase disease. For that, 12 gerbils [Experiment I: not... more
The objective of this study was to evaluate the pathogenesis of ascites in mice infected with Toxoplasma gondii and gerbils infected with Neospora caninum during the acute phase disease. For that, 12 gerbils [Experiment I: not infected/control (n=6) and infected (n=6)] and 12 mice [Experiment II: control (n=6) and infected (n=6)] were used. Infected gerbils and mice showed marked ascites on days 5-7 post-infection (PI), while the not-infected animals had not ascites. Peritoneal liquid was collected from the all mice with uninfected animals receiving 1.5mL of saline solution into their abdominal cavity, allowing the recovery of cavity liquid. As a result, it was possible to observe differences in physics, chemistry and cytological analysis of the fluid cavity of animals infected with N. caninum and T. gondii, when they were compared with uninfected animals, as well as between animals experimentally infected. Additionally both, N. caninum and T gondii, caused an increase in the levels of nitric oxide (NOx-nitrate/nitrite), protein oxidation (AOPP) and lipid peroxidation (TBARS), while serum total protein and albumin were reduced in infected gerbils and mice. Gerbils infected with N. caninum showed multiple large cells with multilobulated nucleus, lytic necrosis and abundant amount of eosinophilic cytoplasm into the hepatic parenchyma. By the other hand, mice infected with T. gondii developed myriad foci of lytic necrosis combined with tachyzoites and cysts containing bradyzoites in liver. Both experimental models for N. caninum and T. gondii showed inflammatory foci and tachyzoites the peritoneum, which could be a major cause of ascites. Toxoplasmosis and neosporosis were able to cause clinical signs in experimental models with similar alterations in peritoneal fluid; however the toxoplasmosis histological changes were much more evident. Therefore, the pathogenesis of ascites appears to be directly related to liver damage, which strongly suggests alteration in the normal production of proteins as observed in this study, along with peritonitis.
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Mammary cystic dysplasia or mastosis is a rare condition that affects adult or old female cats. A case of mammary cystic dysplasia diagnosed in a seven-year-old mixed breed female cat with a history of widespread increased mammary volume... more
Mammary cystic dysplasia or mastosis is a rare condition that affects adult or old female cats. A case of mammary cystic dysplasia diagnosed in a seven-year-old mixed breed female cat with a history of widespread increased mammary volume for more than a year is described. The diagnosis was made based on the clinical, citopathologic, and histopathological changes.
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... al. 1976). Apesar da marcada sobreposição das lesões vesicais nas duas formas crônicas da intoxicação, que será discu-tida mais adiante, clinicamente apenas um bovino apre-sentou sinais clínicos das duas formas. Este ...