RU2570749C1 - Method of treatment of cows with hepatosis - Google Patents

Abstract

FIELD: veterinary medicine.

SUBSTANCE: method comprises pathogenetic therapy and administration of drugs with hepatoprotective action. At that the hepatoprotector is administered orally as hepatonic and extract of sapropel 60, 30 and 15 days before calving in effective doses.

EFFECT: method enables to normalize the functional state of the liver, the overall physiological condition, productivity and natural resistance of cows with hepatosis and calves born by them.

2 cl, 4 tbl, 1 ex

Description

The invention relates to veterinary medicine and can be used in the treatment of highly productive cows with hepatosis.

Many works of domestic and foreign researchers have been devoted to the treatment and prevention of hepatosis in animals, but so far in veterinary practice there are practically no well-established treatment regimens for this disease.

B.V. Usha (1969) sees the detoxification of the body and the maximum strengthening of regenerative processes in the liver, leading to the restoration of its anatomical and functional integrity, as the main goals of the treatment of hepatosis. This can be achieved, on the one hand, by removing toxins and pathological products of interstitial metabolism from the body or neutralizing them, on the other hand, by enriching and quickly replenishing glycogen stores in functioning liver cells.

In the treatment of sick animals with liver pathology, it is advisable to use drugs that regulate metabolism (N.D. Shendrik, 1986; N.I. Kuznetsov, 1995; V.A. Antipov et al., 1997; T.N. Rakova et al. , 1997; V.N. Denisenko, 2002; E.V. Kuzminova et al., 2006), in particular, include vitamins, carotene-containing drugs, amino acids, hormones, trace elements, etc.

B.M. Anokhin, V.M. Danilevsky et al. (1991) for the treatment of animals with hepatosis, mainly recommend the use of lipotropic, vitamin and choleretic drugs.

Of the lipotropic drugs, methionine, lipoic acid, lipamide and others are used.

Similar treatment regimens for animals with hepatosis result in T.T. Shcherbakov, A.V. Korobov et al. (2002).

The most specific methods of treating animals with hepatosis of practical interest are the following: Ribav-5 (Mikovit), a phytomedication of lucevit, Antitox, depolen and NUTRIL ® Se, depolen and vermiculite of Potanensky origin, purivetin and hepatotropic premix hepatitis.

There is a method of correcting the functional state of the liver of cows with hepatosis, by using the drug Ribav-5 (L.G. Kislinskaya, 2000). Rib rib is an alcoholic extract of the products of the synthesis of endophytic micromycetes from ginseng root, containing a balanced complex of biologically active substances (amino acids, peptides, phosphorus-containing compounds, vitamins, enzymes, pigments, lipids, etc.). The pharmacological properties of the drug are determined by the combination of properties that make up its components. The drug is recommended to be used in a dose of 10-15 ml per head inside per os for 10 days in a row. However, this method of treating dairy cows with hepatosis is not technologically advanced, as it requires an individual approach to the animal.

A known method of using a phytopreparation of lucevitis in hepatosis of cows (I.F. Khazimukhametova, P.P. Idrisova, 2008). Cows were treated according to the following scheme: a 20% aqueous solution of lucevit at a dose of 25 mg / kg body weight intramuscularly 1 time per day for three days three times with an interval of three days. The drug contains a number of biologically active substances (sterols, vitamins, amino acids, sugars, macro- and microelements), which are involved in the neutralization of food toxins in the liver and small intestine, have hepatoprotective and choleretic action. However, this method is also not technological, since it requires an individual approach to the animal and does not solve the problem of detoxification of the body from toxic elements, does not exclude the content of heavy metals in the soil, which are contained in the soils where alfalfa is grown.

Antitox - a solution of a hydrolyzate of liver tissue and mineral salts of isotonic concentration. For therapeutic purposes, the drug is injected for 5-6 days in a row to cows at a dose of 20-40 ml, and calves at a dose of 10-12 ml, with 0.5 doses being administered subcutaneously and 0.5 doses intramuscularly (E.V. Dushkin, 2010 ) This method of treatment and prevention of hepatosis in animals can reduce the incidence, increase the safety of the livestock, increase efficiency and reduce treatment time. This method of treating cows and calves with hepatosis also requires an individual approach to the animal and is not technologically advanced.

The effectiveness of the use of selenium-containing preparations of depolen and NUTRIL ® Se for hepatosis of highly productive and dry cows has been proven (N.A. Ferdman, 2007). Depolen contains barium selenate (selenite) and liquid paraffin, NUTRIL ® Se - vitamins (A, E, D ₃ , K ₃ , PP, vitamin C, group B), lysine, methionine, tryptophan and selenium. Depole is administered intramuscularly at 10 ml (in the fifth month of pregnancy), NUTRIL ® Se is fed at a dose of 0.02 g / kg body weight for 10 days (dry cows). This method also requires an individual approach to each animal.

An effective way to treat dairy cows with hepatosis in technogenic provinces with an excess of nickel and lead is symptomatic therapy and the introduction of depolene, which has an antioxidant effect and Potanensky vermiculite, which provides detoxification therapy (A.M. Gertman et al., 2010). Depolen is administered subcutaneously in the neck at a dose of 1.9-2.1 ml per 100 kg of body weight once for the entire treatment period, vermiculite is fed with concentrates at the rate of 0.10-0.15 g / kg of body weight twice a day within 14-15 days with a subsequent interval of 14-15 days. The duration of treatment is 85-90 days.

Vermiculite is a natural mineral of the Potanensky deposit in the Chelyabinsk region from the class of hydromica, the structure of which consists of alternating mica sheets, separated by double layers of water. It includes compounds of silicon, aluminum, iron, manganese, magnesium, calcium, potassium, which allows to fill the lack of essential elements in the body of cows. Expanded vermiculite used in veterinary medicine is a light porous mineral with sorption, catalytic and ion-exchange properties. Due to its high adsorption properties, vermiculite removes salts of heavy metals from the body of cows. At the same time, the macro- and microelements contained in it enter into metabolism and are absorbed by the intestinal wall.

Depolen is a drug containing barium selenite and liquid paraffin. It is a viscous, odorless white suspension that exfoliates during long-term storage. It is a means of antioxidant protection of the body. By reducing and preventing the accumulation of toxic products, lipid peroxidation, it helps to normalize metabolism and increase the resistance of animals. After a single use of depolen, the physiological need of animals for selenium is ensured for a long time. This method of treatment for hepatosis of cows has an effect in technogenic provinces against the background of an excessive content of nickel and lead salts in environmental objects and with balanced feeding.

There is a method for the treatment and prevention of hepatosis in newborn calves through the use of a complex drug, a broad spectrum biostimulant - purivetin, manufactured by ELEST (I.A. Nikulin, Yu.A. Shumilin, 2007). Purivetin is a loose powder of light beige color. It consists of purines (inositol), methyluracil, cotalamide (coenzyme form of vitamin B ₁₂ ), ascorbic acid (vitamin C), fructose-1,6-calcium diphosphate (FDF), citric acid. The pharmacological properties of the drug are determined by the combination of properties that make up its components. The drug is used according to the scheme: the first 5 days - 40 mg / kg, 5 days - 30 mg / kg and the last 10 days - 20 mg / kg. This method has an effect in the treatment of calves with hepatosis, accompanied by myocardial dystrophy.

Hepavet - hepatoprotective premix containing essential phospholipids and sodium bentonite. The pharmacological properties of the premix are determined by the combination of properties that make up its components. The use of hepavet premix for hepatosis in dogs at a dose of 4 g per 10 kg of body weight once a day for 21 days restores the metabolic functions of the liver, which is manifested by an improvement in the general condition of animals, the elimination of pain in the liver, a decrease in the severity of cytolysis and cholestasis syndromes, and normalization of synthesis proteins, pigmentation and erythropoiesis in the organs of blood formation (O.S. Korchagina, 2008). The use of this method for the treatment of hepatosis in dairy cows will not be economically feasible due to the high dose of the drug, the low technological use on a large livestock, and it is not physiological, since ruminants and carnivorous carnivores differ in the structure of internal organs, including the liver.

The aim of the present invention is to develop a method for the treatment of dry cows with hepatosis by introducing a hepatotropic drug hepatonic separately and in combination with sapropel extract into the diet.

This goal is achieved by the fact that deep-growing cows (8th month of pregnancy) are fed hepatonic and sapropel extract 60, 30 and 15 days before calving, respectively, 80 and 300 ml per head 1 time per day for 5 consecutive days. Hepatonic provides detoxification function, normalizes impaired metabolism, prevents the development of fatty hepatosis. Sapropel extract also normalizes metabolism, provides detoxification therapy and antioxidant protection of the body.

A comparative analysis of the claimed solution with the prototypes shows that this method differs from the known methods in that hepatoprotector hepatonic combined with sapropel extract are used as drugs for treating hepatosis cows, which are introduced into the diet of lactating and dead cows according to a certain scheme for 60, 30 and 15 days before calving.

Analysis of the prototypes in this field of veterinary medicine did not reveal signs similar to the claimed solution, which allows us to conclude that the method meets the criterion of "novelty."

The claimed method meets the criteria of the invention "inventive step", as it clearly does not follow from the prior art.

The invention is industrially applicable, since it can be used in veterinary medicine for the treatment and prevention of liver diseases, hypovitaminosis, osteodystrophy, etc. in cows.

Execution example. The method is implemented as follows. Hepatonic is introduced into the diet of cows 60, 30 and 15 days before calving at a dose of 80 ml per head (0.13-0.15 ml / kg body weight) 1 time per day for 5 consecutive days.

Hepatonic ™ is a complex stimulant for internal use. Producer NPP Biofarm, Kharkov. It is a clear pink solution. 100 ml of the drug contain: carnitine chloride - 5 g; magnesium sulfate - 20 g; sorbitol - 25 g; cyancobolamine (vitamin B ₁₂ ) - 3 mg; calcium pantothenate (vitamin B ₅ ) -750 mg; nicotinamide - 2 g; succinic acid - 2 g; auxiliary and formative substances - up to 100 ml. The action of the drug is aimed at improving the metabolic functions of the body in animals and birds, increasing resistance, preventing fatty liver disease, improving feed conversion rates, general metabolic rates.

The drug is used to increase the overall resistance of the body, stimulate metabolism, reduce the risk of developing fatty liver disease, reduce the negative effects of stress during transportation, vaccination, change the diet, etc., as well as to accelerate growth during fattening. The drug improves feed conversion by increasing the digestibility of feed, stimulating digestion and assimilation of nutrients.

Dosage Poultry - 1-2 ml / l of drinking water per day for 5-10 days; pigs: young animals - 1-2 ml / goal per day for 5-10 days; adults - 15-40 ml / goal per day for 5-10 days. If necessary, the course can be extended to 21 days. The drug has not been tested on cattle, which is why it was the basis to develop an optimal way to correct hepatosis in cows using hepatonic.

The second example of execution. The method is implemented as follows. Hepatonic and sapropel extract are introduced into the diet of cows 60, 30 and 15 days before calving: hepatonic 80 ml per head (0.13-0.15 ml / kg body weight), sapropel extract - 300 ml per head (0.50 -0.55 ml / kg body weight) 1 time per day for 5 consecutive days.

Sapropel extract (ES-2) is a biologically active drug for internal and external use, refers to non-specific stimulating drugs of natural origin, has an energy and bacteriostatic effect. It was obtained by extraction with organic extracts harmless at applicable doses from sapropels extracted from lakes in the Omsk Region. Produced by JSC Omsk Biocombine according to the original technology of ZANPO Vega-2000-Siberian Organics. Contains water and alcohol soluble mineral and organic substances (table. 1).

The biologically active substances of the sapropel extract have a local and general effect on the animal organism due to the vitamins B, C, E, D contained in it, a set of essential amino acids, enzymes, humic acids, minerals, hormone-like substances and some other biologically active substances formed in sapropel with anaerobic autolysis of organic residues with minerals in freshwater lakes. The introduction of a metered amount of sapropel into the diet allows you to make up for the lack of essential minerals, vitamins, amino acids and other biologically active substances. When absorbed, energy is converted to glucose, the material necessary for cells to regenerate. This is manifested by the normalization of mineral, vitamin, hormonal metabolism, stimulation of blood formation, a decrease in the background effect of toxic factors on parenchymal organs, in particular, on the liver, and an increase in animal reproduction.

During the use of sapropel extract side effects were not detected.

The studies were carried out on the basis of ZAO Krasnenskoye, Yakovlevsky District, Belgorod Region.

The object of the study were cows of black-motley breed aged 5-6 years, at the 3rd and 4th lactation, live weight of 550-600 kg with a productivity of 6.3 to 7.5 thousand kg of milk for the previous 305 days of lactation .

Initially, our studies were devoted to the diagnosis of the functional state of the liver in pregnant cows by conducting a compulsory medical examination of the livestock. The diets of lactating and dry cows, the clinical condition and productivity of pregnant cows, healthy and sick with subclinical hepatoses, their immunological and biochemical status were analyzed.

Analysis of the diets of lactating and dry cows showed that they were unbalanced in digestible protein, dry matter, sugar, macro- and microelements.

Of the 250 cows examined, 43 heads had pronounced signs of hepatosis, which were characterized by a decrease in appetite, a violation of the chewing process, partial hypotension of the pancreas, and weakening of intestinal motility. Percussion noted an increase in the boundaries of the liver - the posterior border behind the last rib, pain was revealed in individual animals. It should be noted that all animals were average and above average fatness.

The clinical manifestations of hepatosis were confirmed by pathological examination and histological studies of pieces of liver tissue taken during special slaughter of animals in which productive qualities were reduced.

When examining the liver of animals observed changes characteristic of fatty hepatosis, manifested by an increase in the organ, icteric staining of the parenchyma with a clay shade and sagging parenchyma. In some cases, a greasy coating remained on the surface of the knife, in others there was a significant compaction of the tissue. During histological analysis of the selected material, fatty degeneration of hepatocytes was the main one in the morphological picture. Other changes receded into the background. In the studied cows, fatty hepatosis was detected without an inflammatory reaction. At the same time, in the liver there were observed areas of moderate diffuse small-, medium-, large-droplet, predominant intracellular obesity. In some parts of the liver of the studied cows, marked diffuse large-drop obesity with extracellular obesity and the formation of fatty cysts is noted, the mass of hepatocytes is in a state of necrobiosis. In significant areas of the liver, thickening of individual portal tracts is expressed. The structure of the hepatic lobules is broken, they are disconnected and surrounded by wide layers of connective tissue - septa. Such false lobules represent part of the hepatic acini; they do not contain portal tracts and central veins. In general, the described picture characterizes the emerging small-node monolobular cirrhosis of the liver.

Hematological studies found that in whole blood the most significant differences between sick cows and healthy cows, taking into account the natural variability of the indicators, should be considered a reduced content of red blood cells by 9.9% and hemoglobin by 14.2%, a shift in the leukogram towards an increase in the proportion of stab neutrophils and eosinophils, as well as a decrease in glucose levels of 41.6%, which is a consequence of toxicosis. In the blood serum of sick cows, the total protein content exceeded the norm by 7.0%, the level of β- and γ-globulins by 7.6 and 12.4%, respectively. An increase in the concentration of these fractions of the total protein against a background of a 14.0% decrease in the albumin fraction indicates damage to the liver cells - hepatocytes with toxic elements. This is also confirmed by an increase in the activity of the main transamination enzymes AsAT and AlAT, which exceeded the standard value by 36.6 and 61.7%, respectively.

Changes in the protein composition of the blood were also confirmed by a colloidal sedimentary test: a positive test was observed in 24% of the cows, and a sharply positive test in 22%, which corresponded to the highest degree of impaired liver morphology and function.

As for the increase in the activity of alkaline phosphatase by 85.0%, this may indicate the presence of cholestasis accompanying hepatosis.

The urea level in the blood serum of sick animals was 19.5% lower than that of healthy animals.

In sick animals, fat and, as a consequence, lipoid metabolism are inhibited: the content of total lipids is 5.3% lower than normal. An increase in cholesterol synthesis by 22.8% was established, which mainly reflects the state of the liver, where it is synthesized, and indicates an increased influx of fat from fat depots into the liver and its fatty infiltration.

The activation of alternative processes also explains the increase in blood serum of total bilirubin by 36.6% (damaged bile capillaries pass bile into the sinusoids of the lobules, then into the central vein of the lobule and into the general circulation).

The calcium content in the blood of sick cows decreased by 13.8% compared with clinically healthy ones, phosphorus - by 28.3% more, which is explained by the excessive content of phosphorus in the diet, the excess of which is not excreted, but circulated in the blood.

Protein overfeeding leads to the intensive development of lactic acid fermentation in the rumen, which leads to abundant formation of lactic acid, which causes a pH shift to the acid side, a decrease in the number of ciliates by 41.7% and the concentration of volatile fatty acids (VFA) by 8.2% compared with healthy animals.

In a general urine test, nine out of thirty sick cows showed glucose (2.7 mmol / L), but its presence can be interpreted as physiological glucosuria, which is characteristic of the pregnant cows on which the studies were conducted; the concentration of ketone bodies in urine exceeded the upper permissible limit of normal by 2 times (2.0 ± 0.2 mmol / l). Urobilinogen (16 mmol / L) was found in six cows, and bilirubin (+) in twenty-one. The appearance of bilirubin in the urine indicates that liver cells are destroyed, and direct bilirubin penetrates the blood, is introduced into the kidneys and excreted from the body.

Thus, according to the complex of these changes, supplemented by the corresponding history data, we diagnosed hepatosis in pregnant dairy cows.

In experimental studies on the effectiveness of hepatonics in combination with sapropel extract in the treatment of cows with hepatosis, 30 animals were included at the 7th month of pregnancy.

Animals according to the principle of analog pairs were divided into 3 groups (control and two experimental), 10 animals each.

The cows of the control group received the main diet and, as pathogenetic therapy, they were injected intravenously with a 10% glucose solution at a dose of 100-150 ml, a 10% solution of calcium chloride and intramuscularly: trivit - 10 ml once every 10 days and 20% ascorbic acid - 5 ml once a day for 10 days.

Animals of the first experimental group, in addition to the main diet 60, 30 and 15 days before calving, received hepatonic orally 80 ml once a day for 5 consecutive days.

To the cows of the second experimental group for therapeutic purposes, hepatonic and sapropel extract were also added to the main diet 60, 30, and 15 days before calving, 80 and 300 ml respectively, respectively, once a day for 5 consecutive days.

Given the basic principle of therapy - complexity - the cows of both experimental groups were injected with a 10% glucose solution at a dose of 100-150 ml, a 10% solution of calcium chloride and intramuscularly as pathogenetic therapy: trivit - 10 ml once every 10 days and 20% ascorbic acid - 5 ml once a day for 10 days.

During a repeated clinical study of sick cows of the experimental groups (30 days before calving), it was found that the process of chewing and burping, the functioning of the pre-stomachs occur in physiologically normal mode. The liver of animals is painless, not enlarged, the area of hepatic dullness is revealed on the right side in the upper part of the X-XII intercostal space in the form of an irregular quadrangle. In contrast to the cows of the experimental groups, the symptoms of hepatosis persisted in sick animals of the control group.

Thus, the use of hepatonics separately and in combination with sapropel extract for hepatosis-infected cows helps to improve the clinical performance of cows and activate the gastrointestinal tract. This indicates an increase in metabolic processes in the body.

The criterion for assessing the effectiveness of the therapeutic effect of hepatonics and sapropel extract in comparison with the control was the results of morphological and biochemical studies of blood, urine and scar contents.

The normalization of erythropoiesis is indicated by an increase in the number of red blood cells in the blood of cows of the first and second experimental groups relative to the control group by 21.8 and 32.8%), hemoglobin by 2.6 and 11.6%, which in turn affected a decrease in ESR by 5 , 1 and 23.0%.

In the leukocyte population in animals of both experimental groups, the proportion of eosinophils decreased to normal by 51.0 and 36.5%, as well as the fraction of stab neutrophils, respectively, by 32.8 and 44.8%. The proportion of segmented neutrophils increased by 19.7 and 29.8%. There was a tendency to increase the proportion of lymphocytes and lower monocytes. The best hematological parameters were in the second experimental group, where hepatonic was used in combination with sapropel extract. The change in biochemical blood parameters are presented in table 2.

From the data of table 2 it can be seen that the content of total protein in the blood serum decreased by 4.8 and 7.1%, which indirectly indicates the normalization of liver function, the restoration of its parenchyma. In the total protein, the proportion of albumin increased by 35.1 and 37.4%. There was a tendency and increase in the proportion of α-globulins. The proportion of β-globulins decreased in the first and second experimental groups by 34.5 and 38.8%), and γ-globulins - by 11.2 and 14.1%. The content of urea, cholesterol and glucose in the first and second experimental groups significantly increased in relation to the control, respectively, by 70.1 and 80.6%, 80.9 and 98.8%, 22.8 and 24.4%.

The content of total bilirubin decreased by 28.9 and 44.2%, which indicates the normalization of pigment metabolism.

In the blood serum of both experimental groups of cows, calcium also normalized: phosphorus ratio.

The activity of aspartate aminotransferase (AsAT) in the blood serum of animals of the first and second experimental groups decreased by 15.4 and 19.9%. Alanine aminotransferase (AlAT) activity significantly decreased in the second experimental group by 29.4% and reached the physiological norm.

A decrease in the activity of indicator enzymes indicates the absence of destruction of liver cells.

Three days after calving, the clinical indicators of experimental cows were within normal limits and corresponded to the physiological state of the animals. However, in comparison with sick cows of the control group, animals of the first and second experimental groups noted a decrease in heart rate and respiration, as well as an increase in scar reduction by 60.0 and 55.0%.

This indirectly indicates an increase in metabolic processes in animals.

A positive trend in the morphological and biochemical composition of the blood continued.

The use of hepatonics and sapropel extract for cows with hepatosis patients had a positive effect on the state of their natural resistance (Table 3).

From the data of table 3 it is seen that in the blood serum of cows of the first and second experimental groups, lysozyme activity was higher than the control by 31.4 and 33.9%, bactericidal activity - by 31.0 and 32.3%, phagocytic activity of neutrophils - by 8 , 0 and 8.5%, the phagocytic index - by 31.4 and 35.0% and the phagocytic number - by 38.7 and 49.0%, respectively.

Based on the normalization of the blood picture, the positive effect of hepatonics and sapropel extract on the functions of the liver and the whole organism as a whole has been established, since the liver is the main organ that responds to tissue alteration using the products produced in it.

This is confirmed by the results of a study of the contents of the scar, indicating the normalization of cicatricial digestion in experimental cows, and restoration of the physicochemical properties of urine to normal.

So, the pH of the cicatricial content in patients with cow hepatosis (control group) is 5.27, i.e. shifted to the acidic side relative to the background value in healthy animals by 18.7%). In cows of the first and second experimental groups, this indicator increased slightly acidic and amounted to 6.37 and 6, 52, respectively.

The number of ciliates in the rumen of cows of the first and second experimental groups was significantly higher than the control by 59.0 and 65.6%, respectively. They also noted a tendency towards an increase in the concentration of VFA in relation to the control.

When analyzing urine, it was found that its specific gravity in cows of the second experimental group was 24.6% higher than the control and amounted to 1.040 ± 0.006 g / ml. Urine pH in both experimental groups of cows shifted to slightly alkaline and amounted to 7.46 ± 0.28 and 7.34 ± 0.29, respectively, which is 15.6 and 14.9% lower than the control. The concentration of ketone bodies in the urine of cows of the first and second experimental groups was lower than the control by 47.6 and 42.9%. In the urine of cows of the first and second experimental groups, as in healthy ones (background), glucose, bilirubin and urobilinogen were not found, which indicates the restoration of liver function.

Cows calving took place mainly in the expected time with deviations in some cases by 4-5 days. The postpartum status of experimental cows is shown in table 4.

From the data of table 4 it can be seen that in the first experimental group, one cow (10%) had a delay in the placenta, and she was later diagnosed with endometritis. One cow from the second experimental group developed ovarian hypofunction. The treatment was effective: the functions recovered after a double treatment with diamol. In the control group, one cow (10%) aborted, in two (20%) - retention of the placenta and the occurrence of endometritis; three cows were treated for ovarian hypofunction.

The postpartum state had a positive effect on the fertility of experimental cows. So, the time of fruitful insemination decreased from 103 days in the control group to 99 and 88 days, respectively, in the first and second experimental groups. The difference of 4-15 days may affect the course of subsequent lactation and the total productivity of cows.

The cows of the experimental groups are 2 times less than in the control overshots. Thus, the use of hepatonics separately and in combination with sapropel extract contributes to the normalization of postpartum activity and fertilization of cows, reduces the possibility of cow disease with endometritis, which ultimately favorably affects the condition of newborn calves and physiological homeostasis of cows. The best indicators are characteristic of cows of the second experimental group, which were fed hepatonic in combination with sapropel. Newborn calves from cows of the first and second experimental groups were distinguished by a more pronounced physiological maturity. So, their average live weight at birth, respectively, groups 1.7 kg (4.6%) and 1.9 kg (5.1%) higher than in the control group. They were distinguished by a more pronounced motor activity, better nutritional behavior.

Summarizing the obtained data, we can conclude that the claimed method in comparison with other methods proposed in this area allows the use of a complex stimulating drug hepatonic in the treatment of dry milk cows with hepatosis, at the rate of 80 ml per head (0.13-0 , 15 ml / kg body weight) in combination with sapropel extract at a dose of 300 ml per head (0.50-0.55 ml / kg body weight) once a day for 5 consecutive days 60, 30 and 15 days before calving.

Information sources

1. Antipov V.A. Drug premixes in animal husbandry and veterinary medicine / V.A. Antipov, M.P. Semenenko, SL. Sokolovsky // Ecological problems of pathology, pharmacology and therapy. - Voronezh, 1997 .-- S. 176.

2. Internal non-communicable diseases of farm animals / B.M. Anokhin, V.M. Danilevsky, L.G. Zamarin et al .: Ed. V.M. Danilevsky. - M .: Agropromizdat, 1991 .-- S. 261-265.

3. Internal diseases of animals / Ed. G.G. Shcherbakova, A.V. Korobov. - St. Petersburg: Doe, 2002 .-- S. 199-203.

4. Gertman A.M. A method for the treatment of hepatosis of dairy cows in a technogenic province with an excess of nickel and lead / A.M. Gertman, D.M. Maksimovich, T.S. Kirsanova. RF patent for the invention No. 2402323. - Declared. 04/06/2009. Publ. 10/27/2010.

5. Denisenko V.N. Diagnosis, treatment and prevention of liver diseases in animals: Lecture. - M .: MGAVMiB them. K.I. Scriabin, 2002 .-- 50 p.

6. Dushkin E.V. A method for the treatment and prevention of hepatosis in cows. RF patent for the invention No. 2385728. - Declared. 10/20/2009. Publ. 04/10/2010.

7. Kislinskaya L.G. The scoring principle for assessing the state of the body of dairy cows and the use of ribava-5 in hepatosis: Abstract. diss .... cand. vet. sciences. - Orenburg, 2000 .-- 20 p.

8. Korchagina O.S. Diagnosis and treatment of hepatosis in service dogs: Abstract. diss .... cand. vet. sciences. - Voronezh, 2008 .-- 23 p.

9. Kuznetsov N.I. Biologically active substances for the prevention and treatment of diseases of metabolic disorders and normalization of liver function in animals // Results and prospects of scientific research on the problems of animal pathology and the development of means and methods of therapy and prevention: Proc. Mat. meeting / N.I. Kuznetsov. - Voronezh: VNIVIPFiT, 1995 .-- S. 41-45.

10. Kuzminova E.V., Zholobova I.S., Zafiridi A.G. Normalization of liver function in cattle // Veterinary pathology. - Moscow, 2006.-№2 (17) .- S. 140-141.

11. Nikulin I.A. The use of purivetin for the treatment of hepatosis of newborn calves / I.A. Nikulin, Yu.A. Shumilin // Veterinarian. - 2007.-№1. - S. 37-39.

12. Rakova T.N., Voronina M.V., Litchenko M.N. Hepatoprotective properties of an environmentally friendly drug from honey - vitamedine // Ecological problems of pathology, pharmacology and animal therapy: Proceedings of the international. coordination meeting. - Voronezh, 1997 .-- S. 249-250.

13. Usha B.V. Diseases of the liver of cattle. - M .: Kolos, 1969.-112 p.

14. Ferdman N.A. The effectiveness of selenium preparations in hepatosis of cows: Author's abstract. diss ... cand. Vet.Science / N.A. Ferdman. - Yekaterinburg, 2007 .-- 18 p.

15. Khazimukhametova I.F. Treatment of cows with hepatosis / I.F. Khazimukhametova, P.P. Idrisova. - Veterinary medicine. - 2008. - No. 5. - S. 39-42.

16. Shendrick N.D. The effectiveness of the introduction of vitamins A and E in patients with hepatosis to cows in different ways // Veterinary medicine. - 1986. - No. 1. - S. 63-64.

Claims (2)

1. A method of treating dry cows with hepatosis, including pathogenetic therapy and the administration of a drug with hepatoprotective action, characterized in that hepatonic is used as a hepatoprotector 60, 30 and 15 days before calving, 80 ml per head inside (0.13-0 , 15 ml / kg body weight) 1 time per day for 5 consecutive days. 2. A method for the treatment of dry cows with hepatosis according to claim 1, characterized in that hepatonic is used together with sapropel extract 60, 30 and 15 days before calving inside: hepatonic 80 ml per head (0.13- 0.15 ml / kg body weight), sapropel extract - 300 ml per head (0.50-0.55 ml / kg body weight) 1 time per day for 5 consecutive days.