RU2003136429A - Способ секвенирования полинуклеотидов - Google Patents
Способ секвенирования полинуклеотидов Download PDFInfo
- Publication number
- RU2003136429A RU2003136429A RU2003136429/13A RU2003136429A RU2003136429A RU 2003136429 A RU2003136429 A RU 2003136429A RU 2003136429/13 A RU2003136429/13 A RU 2003136429/13A RU 2003136429 A RU2003136429 A RU 2003136429A RU 2003136429 A RU2003136429 A RU 2003136429A
- Authority
- RU
- Russia
- Prior art keywords
- enzyme
- polynucleotide
- immobilized
- molecule
- solid carrier
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims abstract 27
- 102000040430 polynucleotide Human genes 0.000 title claims abstract 12
- 108091033319 polynucleotide Proteins 0.000 title claims abstract 12
- 239000002157 polynucleotide Substances 0.000 title claims abstract 12
- 238000012163 sequencing technique Methods 0.000 title 1
- 108090000790 Enzymes Proteins 0.000 claims abstract 15
- 102000004190 Enzymes Human genes 0.000 claims abstract 15
- 230000000694 effects Effects 0.000 claims abstract 6
- 230000003287 optical effect Effects 0.000 claims abstract 5
- 230000003993 interaction Effects 0.000 claims abstract 3
- 239000007787 solid Substances 0.000 claims 7
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 claims 3
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 claims 3
- 229910052737 gold Inorganic materials 0.000 claims 3
- 239000010931 gold Substances 0.000 claims 3
- 239000002105 nanoparticle Substances 0.000 claims 3
- 229910052709 silver Inorganic materials 0.000 claims 3
- 239000004332 silver Substances 0.000 claims 3
- -1 dCTP nucleoside triphosphates Chemical class 0.000 claims 2
- 239000002184 metal Substances 0.000 claims 2
- 229910052751 metal Inorganic materials 0.000 claims 2
- 239000002777 nucleoside Substances 0.000 claims 2
- 239000001226 triphosphate Substances 0.000 claims 2
- 235000011178 triphosphate Nutrition 0.000 claims 2
- 102000016559 DNA Primase Human genes 0.000 claims 1
- 108010092681 DNA Primase Proteins 0.000 claims 1
- 108010043121 Green Fluorescent Proteins Proteins 0.000 claims 1
- 102000004144 Green Fluorescent Proteins Human genes 0.000 claims 1
- 108060004795 Methyltransferase Proteins 0.000 claims 1
- 238000001069 Raman spectroscopy Methods 0.000 claims 1
- 238000004630 atomic force microscopy Methods 0.000 claims 1
- 230000000903 blocking effect Effects 0.000 claims 1
- 239000012876 carrier material Substances 0.000 claims 1
- SUYVUBYJARFZHO-RRKCRQDMSA-N dATP Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-RRKCRQDMSA-N 0.000 claims 1
- SUYVUBYJARFZHO-UHFFFAOYSA-N dATP Natural products C1=NC=2C(N)=NC=NC=2N1C1CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-UHFFFAOYSA-N 0.000 claims 1
- HAAZLUGHYHWQIW-KVQBGUIXSA-N dGTP Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 HAAZLUGHYHWQIW-KVQBGUIXSA-N 0.000 claims 1
- NHVNXKFIZYSCEB-XLPZGREQSA-N dTTP Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C1 NHVNXKFIZYSCEB-XLPZGREQSA-N 0.000 claims 1
- 230000002255 enzymatic effect Effects 0.000 claims 1
- 239000005090 green fluorescent protein Substances 0.000 claims 1
- 238000003384 imaging method Methods 0.000 claims 1
- 239000002082 metal nanoparticle Substances 0.000 claims 1
- 238000004651 near-field scanning optical microscopy Methods 0.000 claims 1
- 230000010355 oscillation Effects 0.000 claims 1
- 125000005504 styryl group Chemical group 0.000 claims 1
- 239000000126 substance Substances 0.000 claims 1
- 238000004416 surface enhanced Raman spectroscopy Methods 0.000 claims 1
- 238000002198 surface plasmon resonance spectroscopy Methods 0.000 claims 1
- 238000012800 visualization Methods 0.000 claims 1
- 238000005259 measurement Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y5/00—Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Immunology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Claims (27)
1. Способ определения последовательности полинуклеотида, предусматривающий стадии
(i) взаимодействия фермента, осуществляющего процессинг полинуклеотида, иммобилизованного в фиксированном положении, с полинуклеотидом-мишенью при условиях, обеспечивающих ферментативную активность;
(ii) обнаружение эффекта, являющегося результатом взаимодействия фермента с полинуклеотидом,
причем эффект обнаруживают путем измерения нелинейного оптического сигнала или линейного сигнала, связанного с нелинейным сигналом.
2. Способ по п.1, при котором эффект обнаруживают путем измерения нелинейного сигнала.
3. Способ по п.1 или 2, при котором нелинейное оптическое определение представляет собой визуализацию генерации второго или третьего колебания.
4. Способ по п.1 или 2, при котором нелинейное оптическое определение представляет собой спектроскопию комбинационного светорассеяния или поверхностную усиленную спектроскопию комбинационного светорассеяния.
5. Способ по п.1 или 2, при котором диполярную молекулу помещают на фермент или вблизи фермента.
6. Способ по п.5, при котором молекула представляет собой молекулу стирилового красителя.
7. Способ по п.5, при котором молекула представляет собой зеленый флуоресцентный белок.
8. Способ по п.5, при котором диполярная молекула присоединена к отдельным основаниям полинуклеотида.
9. Способ по п.1 или 2, при котором фермент является полимеразой.
10. Способ по п.1 или п.2, при котором фермент является геликазой или праймазой.
11. Способ по п.10, при котором стадия (i) предусматривает присоединение нуклеозидтрифосфатов dATP, dTTP, dGTP и dCTP.
12. Способ по п.11, при котором нуклеозидтрифосфаты содержат одну или несколько блокирующих групп, которые могут быть избирательно удалены импульсным монохроматическим светом.
13. Способ по п.1 или 2, при котором наночастицу металла помещают на ферменте или вблизи фермента.
14. Способ по п.13, при котором наночастица является наночастицей золота или серебра.
15. Способ по п.13, при котором наночастицу включают в одно или несколько отдельных оснований полинуклеотида.
16. Способ по п.1 или 2, при котором фермент иммобилизован на твердом носителе.
17. Способ по п.16, при котором имеется множество ферментов, иммобилизованных на твердом носителе.
18. Способ по п.16, при котором твердый носитель имеет шероховатую металлическую поверхность.
19. Способ по п.17, при котором твердый носитель имеет шероховатую металлическую поверхность.
20. Способ по п.16, при котором носитель является серебром или золотом.
21. Способ по п.17, при котором носитель является серебром или золотом.
22. Способ по п.1 или 2, при котором определение проводят в сочетании с атомной силовой микроскопией или ближнепольной сканирующей оптической микроскопией.
23. Способ по п.1 или 2, который дополнительно предусматривает применение локализованного поверхностного плазмонового резонанса.
24. Способ по п.1 или 2, при котором фермент иммобилизован в фиксированном положении в клетке.
25. Вещество твердого носителя, которое содержит, по крайней мере, одну иммобилизованную полимеразу и, по крайней мере, одну диполярную молекулу, помещенную на полимеразу или вблизи полимеразы.
26. Вещество твердого носителя, которое содержит клетку, иммобилизованную на нем, причем клетка содержит полимеразный фермент, поддерживаемый в фиксированном положении в клетке.
27. Устройство системы визуализации для определения нелинейного оптического сигнала, содержащее твердый носитель, содержащий иммобилизованный на нем фермент, который взаимодействует с полинуклеотидом, и диполярную молекулу, помещенную на фермент или вблизи фермента.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB0112238.1A GB0112238D0 (en) | 2001-05-18 | 2001-05-18 | Sequencing method |
| GB0112238.1 | 2001-05-18 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| RU2003136429A true RU2003136429A (ru) | 2005-05-27 |
| RU2291197C2 RU2291197C2 (ru) | 2007-01-10 |
Family
ID=9914926
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2003136429/13A RU2291197C2 (ru) | 2001-05-18 | 2002-05-20 | Способ секвенирования полинуклеотидов |
Country Status (20)
| Country | Link |
|---|---|
| US (3) | US9476094B2 (ru) |
| EP (1) | EP1389241B1 (ru) |
| JP (2) | JP4094961B2 (ru) |
| KR (1) | KR20030094412A (ru) |
| CN (1) | CN1537172B (ru) |
| AT (1) | ATE383442T1 (ru) |
| AU (1) | AU2002257933B2 (ru) |
| BR (1) | BR0209841A (ru) |
| CA (1) | CA2447222C (ru) |
| DE (1) | DE60224524T2 (ru) |
| DK (1) | DK1389241T3 (ru) |
| ES (1) | ES2299569T3 (ru) |
| GB (1) | GB0112238D0 (ru) |
| IL (1) | IL158743A0 (ru) |
| IS (1) | IS7039A (ru) |
| MX (1) | MXPA03010411A (ru) |
| NZ (1) | NZ529349A (ru) |
| PT (1) | PT1389241E (ru) |
| RU (1) | RU2291197C2 (ru) |
| WO (1) | WO2002095070A2 (ru) |
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-
2001
- 2001-05-18 GB GBGB0112238.1A patent/GB0112238D0/en not_active Ceased
-
2002
- 2002-05-20 AU AU2002257933A patent/AU2002257933B2/en not_active Ceased
- 2002-05-20 CN CN028101456A patent/CN1537172B/zh not_active Expired - Lifetime
- 2002-05-20 AT AT02727736T patent/ATE383442T1/de not_active IP Right Cessation
- 2002-05-20 MX MXPA03010411A patent/MXPA03010411A/es unknown
- 2002-05-20 EP EP02727736A patent/EP1389241B1/en not_active Expired - Lifetime
- 2002-05-20 US US10/478,036 patent/US9476094B2/en active Active
- 2002-05-20 NZ NZ529349A patent/NZ529349A/en unknown
- 2002-05-20 ES ES02727736T patent/ES2299569T3/es not_active Expired - Lifetime
- 2002-05-20 WO PCT/GB2002/002345 patent/WO2002095070A2/en active IP Right Grant
- 2002-05-20 BR BR0209841-5A patent/BR0209841A/pt not_active IP Right Cessation
- 2002-05-20 KR KR10-2003-7014760A patent/KR20030094412A/ko not_active Withdrawn
- 2002-05-20 DK DK02727736T patent/DK1389241T3/da active
- 2002-05-20 RU RU2003136429/13A patent/RU2291197C2/ru not_active IP Right Cessation
- 2002-05-20 JP JP2002591533A patent/JP4094961B2/ja not_active Expired - Fee Related
- 2002-05-20 PT PT02727736T patent/PT1389241E/pt unknown
- 2002-05-20 DE DE60224524T patent/DE60224524T2/de not_active Expired - Fee Related
- 2002-05-20 CA CA2447222A patent/CA2447222C/en not_active Expired - Lifetime
- 2002-05-20 IL IL15874302A patent/IL158743A0/xx unknown
-
2003
- 2003-11-17 IS IS7039A patent/IS7039A/is unknown
-
2007
- 2007-11-14 JP JP2007295468A patent/JP4907498B2/ja not_active Expired - Fee Related
-
2008
- 2008-09-08 US US12/206,536 patent/US20090029383A1/en not_active Abandoned
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2016
- 2016-09-29 US US15/280,636 patent/US20170022556A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| ATE383442T1 (de) | 2008-01-15 |
| EP1389241A2 (en) | 2004-02-18 |
| CA2447222A1 (en) | 2002-11-28 |
| PT1389241E (pt) | 2008-04-17 |
| IL158743A0 (en) | 2004-05-12 |
| DE60224524D1 (de) | 2008-02-21 |
| WO2002095070A2 (en) | 2002-11-28 |
| AU2002257933B2 (en) | 2006-08-10 |
| IS7039A (is) | 2003-11-17 |
| US9476094B2 (en) | 2016-10-25 |
| EP1389241B1 (en) | 2008-01-09 |
| CA2447222C (en) | 2012-09-18 |
| US20090029383A1 (en) | 2009-01-29 |
| KR20030094412A (ko) | 2003-12-11 |
| CN1537172B (zh) | 2013-02-20 |
| JP2004535800A (ja) | 2004-12-02 |
| DK1389241T3 (da) | 2008-05-26 |
| MXPA03010411A (es) | 2004-06-25 |
| US20170022556A1 (en) | 2017-01-26 |
| ES2299569T3 (es) | 2008-06-01 |
| HK1061047A1 (en) | 2004-09-03 |
| JP4094961B2 (ja) | 2008-06-04 |
| JP2008104463A (ja) | 2008-05-08 |
| JP4907498B2 (ja) | 2012-03-28 |
| US20040241678A1 (en) | 2004-12-02 |
| DE60224524T2 (de) | 2009-01-08 |
| CN1537172A (zh) | 2004-10-13 |
| NZ529349A (en) | 2006-10-27 |
| RU2291197C2 (ru) | 2007-01-10 |
| WO2002095070A3 (en) | 2003-09-18 |
| BR0209841A (pt) | 2006-02-07 |
| GB0112238D0 (en) | 2001-07-11 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MM4A | The patent is invalid due to non-payment of fees |
Effective date: 20070521 |