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NZ309777A - Method for the detection of compounds that modulate the effects of the obese protein - Google Patents

Method for the detection of compounds that modulate the effects of the obese protein

Info

Publication number
NZ309777A
NZ309777A NZ309777A NZ30977796A NZ309777A NZ 309777 A NZ309777 A NZ 309777A NZ 309777 A NZ309777 A NZ 309777A NZ 30977796 A NZ30977796 A NZ 30977796A NZ 309777 A NZ309777 A NZ 309777A
Authority
NZ
New Zealand
Prior art keywords
protein
compound
detection
effect
modulate
Prior art date
Application number
NZ309777A
Inventor
Lee James Beeley
Richard Anthony Godwin Smith
Original Assignee
Smithkline Beecham Plc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB9510857.7A external-priority patent/GB9510857D0/en
Priority claimed from GBGB9511602.6A external-priority patent/GB9511602D0/en
Application filed by Smithkline Beecham Plc filed Critical Smithkline Beecham Plc
Publication of NZ309777A publication Critical patent/NZ309777A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/5759—Products of obesity genes, e.g. leptin, obese (OB), tub, fat

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Toxicology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Child & Adolescent Psychology (AREA)
  • Obesity (AREA)
  • Endocrinology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

escribed is a method for the detection of a compound that mimics, potentiates or inhibits the physiological effect of the ob-protein. The method comprises: (a) for a compound which mimics the physiological effect of the ob-protein, assessing for the effect of the compound upon an ob-protein activated signal transducer and activator of transcription (STAT) DNA response element coupled to a reporter gene; or (b) for a compound which potentiates or inhibits the physiological effect of the ob-protein assessing the effect of the compound upon the response provided by ob-protein upon an ob-protein activated STAT DNA response element coupled to a reporter gene; the response element and the reporter being expressed in an ob-protein responsive cell line; a kit of parts adapted for use in such method and a compound when identified by such method.
NZ309777A 1995-05-30 1996-05-28 Method for the detection of compounds that modulate the effects of the obese protein NZ309777A (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB9510857.7A GB9510857D0 (en) 1995-05-30 1995-05-30 Novel assay
GBGB9511602.6A GB9511602D0 (en) 1995-06-08 1995-06-08 Novel assay
PCT/EP1996/002291 WO1996038586A1 (en) 1995-05-30 1996-05-28 Method for the detection of compounds that modulate the effects of the obese protein

Publications (1)

Publication Number Publication Date
NZ309777A true NZ309777A (en) 2000-01-28

Family

ID=26307107

Family Applications (1)

Application Number Title Priority Date Filing Date
NZ309777A NZ309777A (en) 1995-05-30 1996-05-28 Method for the detection of compounds that modulate the effects of the obese protein

Country Status (15)

Country Link
EP (1) EP0832284A1 (en)
JP (1) JPH11505725A (en)
KR (1) KR19990022134A (en)
CN (1) CN1192248A (en)
AU (1) AU715215B2 (en)
BR (1) BR9608686A (en)
CA (1) CA2222409A1 (en)
CZ (1) CZ379097A3 (en)
HU (1) HUP9801744A3 (en)
MX (1) MX9709360A (en)
NO (1) NO975504D0 (en)
NZ (1) NZ309777A (en)
PL (1) PL323638A1 (en)
TR (1) TR199701469T1 (en)
WO (1) WO1996038586A1 (en)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6001968A (en) 1994-08-17 1999-12-14 The Rockefeller University OB polypeptides, modified forms and compositions
US6429290B1 (en) 1994-08-17 2002-08-06 The Rockefeller University OB polypeptides, modified forms and derivatives
CA2252443A1 (en) * 1996-04-22 1997-10-30 Merck & Co., Inc. Leptin assay
US6007998A (en) * 1996-04-22 1999-12-28 Merck & Co., Inc. Leptin assay
US6001816A (en) * 1996-06-20 1999-12-14 Merck & Co., Inc. Gene therapy for leptin deficiency
EP0951564A1 (en) * 1996-11-01 1999-10-27 Smithkline Beecham Plc Method for the detection of compounds that modulate the effects of the obese (ob) protein
IT1293533B1 (en) * 1997-07-14 1999-03-01 Angeletti P Ist Richerche Bio METHOD FOR THE SELECTION OF MOLECULES ABLE TO MIMIC, INHIBIT OR ENHANCE THE EFFECTS OF THE INTERACTION BETWEEN LEPTIN AND CELLS THAT
WO1999023493A1 (en) * 1997-10-31 1999-05-14 The Rockefeller University Methods of identifying agents that modulate leptin activity
CA2320226A1 (en) * 1998-02-11 1999-08-19 Beth Israel Deaconess Medical Center Methods and compositions for modulating leptin activity
GB9814199D0 (en) * 1998-06-30 1998-08-26 Univ Buckingham Novel method
GB9828087D0 (en) * 1998-12-18 1999-02-17 Smithkline Beecham Plc Novel method

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU7795094A (en) * 1993-09-15 1995-04-03 New York University Dna sequence which binds transcriptional regulatory proteins activated in response to various cytokines and uses thereof
EP0722497A1 (en) * 1994-04-14 1996-07-24 Ligand Pharmaceuticals, Inc. Dna spacer regulatory elements responsive to cytokines and methods for their use

Also Published As

Publication number Publication date
EP0832284A1 (en) 1998-04-01
JPH11505725A (en) 1999-05-25
CA2222409A1 (en) 1996-12-05
HUP9801744A2 (en) 1998-10-28
AU6002396A (en) 1996-12-18
NO975504L (en) 1997-11-28
WO1996038586A1 (en) 1996-12-05
PL323638A1 (en) 1998-04-14
HUP9801744A3 (en) 2000-06-28
NO975504D0 (en) 1997-11-28
CZ379097A3 (en) 1998-05-13
CN1192248A (en) 1998-09-02
AU715215B2 (en) 2000-01-20
KR19990022134A (en) 1999-03-25
MX9709360A (en) 1998-02-28
BR9608686A (en) 1999-07-06
TR199701469T1 (en) 1998-03-21

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