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NZ335489A - Method for the detection of compounds that modulate the effects of the obese (OB) protein - Google Patents

Method for the detection of compounds that modulate the effects of the obese (OB) protein

Info

Publication number
NZ335489A
NZ335489A NZ335489A NZ33548997A NZ335489A NZ 335489 A NZ335489 A NZ 335489A NZ 335489 A NZ335489 A NZ 335489A NZ 33548997 A NZ33548997 A NZ 33548997A NZ 335489 A NZ335489 A NZ 335489A
Authority
NZ
New Zealand
Prior art keywords
cell line
protein
derived cell
compound
response element
Prior art date
Application number
NZ335489A
Inventor
Lee James Beeley
Original Assignee
Smithkline Beecham P
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB9622867.1A external-priority patent/GB9622867D0/en
Priority claimed from GBGB9622850.7A external-priority patent/GB9622850D0/en
Priority claimed from GBGB9622868.9A external-priority patent/GB9622868D0/en
Priority claimed from GBGB9622849.9A external-priority patent/GB9622849D0/en
Priority claimed from GBGB9622869.7A external-priority patent/GB9622869D0/en
Priority claimed from GBGB9622865.5A external-priority patent/GB9622865D0/en
Priority claimed from GBGB9622847.3A external-priority patent/GB9622847D0/en
Priority claimed from GBGB9622870.5A external-priority patent/GB9622870D0/en
Priority claimed from GBGB9622866.3A external-priority patent/GB9622866D0/en
Priority claimed from GBGB9622851.5A external-priority patent/GB9622851D0/en
Priority claimed from GBGB9622848.1A external-priority patent/GB9622848D0/en
Application filed by Smithkline Beecham P filed Critical Smithkline Beecham P
Publication of NZ335489A publication Critical patent/NZ335489A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/5759—Products of obesity genes, e.g. leptin, obese (OB), tub, fat
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/72—Receptors; Cell surface antigens; Cell surface determinants for hormones
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Endocrinology (AREA)
  • Toxicology (AREA)
  • Obesity (AREA)
  • Child & Adolescent Psychology (AREA)
  • Cell Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

A method for the detection of a compound that mimics, potentiates or inhibits the physiological effect of the ob-protein comprises: a) for a compound which mimics the physiological effect of the ob-protein: assessing the effect of the compound upon an ob-protein activated signal transducer and activator of transcription (STAT) DNA response element coupled to a reporter gene or b) for a compound which potentiates or inhibits the physiological effect of the ob-protein: assessing the effect of the compound upon the response provided by ob-protein upon an ob-protein activated STAT DNA response element coupled to a reporter gene; wherein the response element and the reporter are expressed in an ob-protein responsive cell line selected from i) a hypothalamic derived cell line; ii) a pheochromocytoma derived cell line; iii) a haematopoietic derived cell line; iv) a pancreatic derived cell line; v) a liver derived cell line; vi) a preadipocyte derived cell line; vii) a skeletal muscle derived cell line and viii) an ovarian derived cell line; or the response element and the reporter are expressed in a cell line which is also transfected with a polypeptide capable of mediating the stimulation by ob-protein of an ob-protein activated STAT DNA response element and contains the appropriate STAT proteins.
NZ335489A 1996-11-01 1997-10-30 Method for the detection of compounds that modulate the effects of the obese (OB) protein NZ335489A (en)

Applications Claiming Priority (12)

Application Number Priority Date Filing Date Title
GBGB9622850.7A GB9622850D0 (en) 1996-11-01 1996-11-01 Novel method
GBGB9622868.9A GB9622868D0 (en) 1996-11-01 1996-11-01 Novel method
GBGB9622849.9A GB9622849D0 (en) 1996-11-01 1996-11-01 Novel method
GBGB9622869.7A GB9622869D0 (en) 1996-11-01 1996-11-01 Novel method
GBGB9622867.1A GB9622867D0 (en) 1996-11-01 1996-11-01 Novel method
GBGB9622865.5A GB9622865D0 (en) 1996-11-01 1996-11-01 Novel method
GBGB9622847.3A GB9622847D0 (en) 1996-11-01 1996-11-01 Novel method
GBGB9622870.5A GB9622870D0 (en) 1996-11-01 1996-11-01 Novel method
GBGB9622866.3A GB9622866D0 (en) 1996-11-01 1996-11-01 Novel method
GBGB9622851.5A GB9622851D0 (en) 1996-11-01 1996-11-01 Novel method
GBGB9622848.1A GB9622848D0 (en) 1996-11-01 1996-11-01 Novel method
PCT/GB1997/002988 WO1998020158A1 (en) 1996-11-01 1997-10-30 Method for the detection of compounds that modulate the effects of the obese (ob) protein

Publications (1)

Publication Number Publication Date
NZ335489A true NZ335489A (en) 2001-01-26

Family

ID=27581686

Family Applications (1)

Application Number Title Priority Date Filing Date
NZ335489A NZ335489A (en) 1996-11-01 1997-10-30 Method for the detection of compounds that modulate the effects of the obese (OB) protein

Country Status (13)

Country Link
EP (1) EP0951564A1 (en)
JP (1) JP2001503982A (en)
KR (1) KR20000053007A (en)
AR (1) AR010262A1 (en)
AU (1) AU4789297A (en)
CA (1) CA2270431A1 (en)
CZ (1) CZ149999A3 (en)
IL (1) IL129685A0 (en)
NO (1) NO992107L (en)
NZ (1) NZ335489A (en)
PL (1) PL333187A1 (en)
TR (1) TR199900963T2 (en)
WO (1) WO1998020158A1 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IT1293533B1 (en) * 1997-07-14 1999-03-01 Angeletti P Ist Richerche Bio METHOD FOR THE SELECTION OF MOLECULES ABLE TO MIMIC, INHIBIT OR ENHANCE THE EFFECTS OF THE INTERACTION BETWEEN LEPTIN AND CELLS THAT
GB9814199D0 (en) * 1998-06-30 1998-08-26 Univ Buckingham Novel method
CA2335107C (en) * 1998-07-28 2010-01-19 Vlaams Interuniversitair Instituut Voor Biotechnologie Vzw Leptin-mediated gene-induction
GB9828087D0 (en) * 1998-12-18 1999-02-17 Smithkline Beecham Plc Novel method
JP4756813B2 (en) 2000-05-22 2011-08-24 フラームス・インテルウニフェルシタイル・インステイチュート・フォール・ビオテヒノロヒー・ヴェーゼットウェー（ヴェーイーベー・ヴェーゼットウェー） Capturing interactions using receptors
GB0019327D0 (en) * 2000-08-08 2000-09-27 Buchanan Baillie Hamilton Paul Slimming system
EP1812038A1 (en) 2004-11-18 2007-08-01 VIB vzw Fibronectin iii domain as leptin receptor antagonists

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH09500026A (en) * 1994-04-14 1997-01-07 リガンド・ファーマシューティカルズ・インコーポレーテッド Cytokine-responsive DNA spacer regulatory element and method of use thereof
US5643748A (en) * 1994-09-14 1997-07-01 Progenitor, Inc. HU-B1.219, a novel human hematopoietin receptor
AU5524896A (en) * 1995-03-20 1996-10-08 Institut Pasteur De Lille Modulators of ob gene and screening methods therefor
US5712094A (en) * 1995-03-27 1998-01-27 Ligand Pharmaceuticals, Inc. Methods for detecting modulators of cytokine action
AU715215B2 (en) * 1995-05-30 2000-01-20 Smithkline Beecham Plc Method for the detection of compounds that modulate the effects of the obese protein
JP2000511406A (en) * 1996-04-22 2000-09-05 メルク エンド カンパニー インコーポレーテッド Leptin assay

Also Published As

Publication number Publication date
AR010262A1 (en) 2000-06-07
EP0951564A1 (en) 1999-10-27
CZ149999A3 (en) 1999-10-13
NO992107D0 (en) 1999-04-30
AU4789297A (en) 1998-05-29
NO992107L (en) 1999-06-30
KR20000053007A (en) 2000-08-25
PL333187A1 (en) 1999-11-22
IL129685A0 (en) 2000-02-29
TR199900963T2 (en) 1999-08-23
WO1998020158A1 (en) 1998-05-14
CA2270431A1 (en) 1998-05-14
JP2001503982A (en) 2001-03-27

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