NO315800B1 - Polypeptid fra urin med betegnelse komponent B, fremgangsmate for fremstilling av dette, DNA-molekyl, ekspresjonsvektor, vertscelle, pattedyrscelle, anvendelse av polypeptidet for fremstilling av et medikament samt farmasoytisk preparat - Google Patents

Polypeptid fra urin med betegnelse komponent B, fremgangsmate for fremstilling av dette, DNA-molekyl, ekspresjonsvektor, vertscelle, pattedyrscelle, anvendelse av polypeptidet for fremstilling av et medikament samt farmasoytisk preparat Download PDF

Info

Publication number: NO315800B1
Authority: NO; Norway
Prior art keywords: component; dna; elution; polypeptide; fraction
Prior art date: 1992-12-22

Application number

NO19952494A

Other languages

English (en)

Norwegian (no)

Other versions

NO952494D0 (no

NO952494L (no

Inventor

Antonino Sirna

Original Assignee

Applied Research Systems

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1992-12-22

Filing date

1995-06-21

Publication date

2003-10-27

1995-06-21 Application filed by Applied Research Systems filed Critical Applied Research Systems

1995-06-21 Publication of NO952494D0 publication Critical patent/NO952494D0/no

1995-08-21 Publication of NO952494L publication Critical patent/NO952494L/no

2003-10-27 Publication of NO315800B1 publication Critical patent/NO315800B1/no

Links

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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
- C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
- C07K14/4702—Regulators; Modulating activity
- C07K14/4703—Inhibitors; Suppressors
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
- A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
- C07K14/71—Receptors; Cell surface antigens; Cell surface determinants for growth factors; for growth regulators
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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Pharmacology & Pharmacy (AREA)
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NO19952494A 1992-12-22 1995-06-21 Polypeptid fra urin med betegnelse komponent B, fremgangsmate for fremstilling av dette, DNA-molekyl, ekspresjonsvektor, vertscelle, pattedyrscelle, anvendelse av polypeptidet for fremstilling av et medikament samt farmasoytisk preparat NO315800B1 (no)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
ITRM920919A IT1257184B (it)	1992-12-22	1992-12-22	Preparato ad attivita' antinfiammatoria, anticoagulante e antitumorale
PCT/EP1993/003645 WO1994014959A1 (en)	1992-12-22	1993-12-21	New protein from urine named component b

Publications (3)

Publication Number	Publication Date
NO952494D0 NO952494D0 (no)	1995-06-21
NO952494L NO952494L (no)	1995-08-21
NO315800B1 true NO315800B1 (no)	2003-10-27

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NO19952494A NO315800B1 (no)	1992-12-22	1995-06-21	Polypeptid fra urin med betegnelse komponent B, fremgangsmate for fremstilling av dette, DNA-molekyl, ekspresjonsvektor, vertscelle, pattedyrscelle, anvendelse av polypeptidet for fremstilling av et medikament samt farmasoytisk preparat

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US (1)	US5908827A (sv)
EP (1)	EP0675956B1 (sv)
JP (1)	JP3025014B2 (sv)
KR (1)	KR100193107B1 (sv)
AT (1)	ATE236976T1 (sv)
AU (1)	AU690093B2 (sv)
CA (1)	CA2151156A1 (sv)
DE (1)	DE69332861T2 (sv)
DK (1)	DK0675956T3 (sv)
ES (1)	ES2193152T3 (sv)
FI (2)	FI114478B (sv)
IL (1)	IL108149A (sv)
IT (1)	IT1257184B (sv)
NO (1)	NO315800B1 (sv)
PT (1)	PT675956E (sv)
RU (1)	RU2177480C2 (sv)
UA (1)	UA46702C2 (sv)
WO (1)	WO1994014959A1 (sv)
ZA (1)	ZA939621B (sv)

Families Citing this family (41)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
EA001077B1 (ru) *	1996-04-24	2000-10-30	Апплайд Резеч Системз Арс Холдинг Н.В.	Компонент в, используемый в качестве рубцующего агента, содержащая его фармацевтическая композиция и способ лечения
AU8536698A (en) *	1997-06-09	1998-12-30	Wolf-Georg Forssmann	Lus-1 human protein, its production and use
DE69903037T2 (de) *	1998-07-09	2003-06-05	Applied Research Systems Ars Holding N.V., Curacao	Komponente b als angiogenes mittel zusammen mit humanen wachstumsfaktoren
US6962700B1 (en) *	2000-09-13	2005-11-08	Atopix Pharmaceuticals Corporation	Method of manufacturing immune globulin
ATE494903T1 (de) *	2003-04-16	2011-01-15	Merck Serono Sa	Verwendung von slurp-1 zur behandlung von krankheiten, die durch störungen der acetylcholinerezeptoren ausgelöst werden
US7343581B2 (en) *	2005-06-27	2008-03-11	Tela Innovations, Inc.	Methods for creating primitive constructed standard cells
US7590968B1 (en)	2006-03-01	2009-09-15	Tela Innovations, Inc.	Methods for risk-informed chip layout generation
US8225261B2 (en)	2006-03-09	2012-07-17	Tela Innovations, Inc.	Methods for defining contact grid in dynamic array architecture
US8839175B2 (en)	2006-03-09	2014-09-16	Tela Innovations, Inc.	Scalable meta-data objects
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US7956421B2 (en)	2008-03-13	2011-06-07	Tela Innovations, Inc.	Cross-coupled transistor layouts in restricted gate level layout architecture
US8225239B2 (en)	2006-03-09	2012-07-17	Tela Innovations, Inc.	Methods for defining and utilizing sub-resolution features in linear topology
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US7446352B2 (en)	2006-03-09	2008-11-04	Tela Innovations, Inc.	Dynamic array architecture
US8247846B2 (en)	2006-03-09	2012-08-21	Tela Innovations, Inc.	Oversized contacts and vias in semiconductor chip defined by linearly constrained topology
US9230910B2 (en)	2006-03-09	2016-01-05	Tela Innovations, Inc.	Oversized contacts and vias in layout defined by linearly constrained topology
US8448102B2 (en)	2006-03-09	2013-05-21	Tela Innovations, Inc.	Optimizing layout of irregular structures in regular layout context
US9009641B2 (en)	2006-03-09	2015-04-14	Tela Innovations, Inc.	Circuits with linear finfet structures
US9035359B2 (en)	2006-03-09	2015-05-19	Tela Innovations, Inc.	Semiconductor chip including region including linear-shaped conductive structures forming gate electrodes and having electrical connection areas arranged relative to inner region between transistors of different types and associated methods
US7943967B2 (en)	2006-03-09	2011-05-17	Tela Innovations, Inc.	Semiconductor device and associated layouts including diffusion contact placement restriction based on relation to linear conductive segments
US8658542B2 (en)	2006-03-09	2014-02-25	Tela Innovations, Inc.	Coarse grid design methods and structures
US8653857B2 (en)	2006-03-09	2014-02-18	Tela Innovations, Inc.	Circuitry and layouts for XOR and XNOR logic
US9563733B2 (en)	2009-05-06	2017-02-07	Tela Innovations, Inc.	Cell circuit and layout with linear finfet structures
US7763534B2 (en)	2007-10-26	2010-07-27	Tela Innovations, Inc.	Methods, structures and designs for self-aligning local interconnects used in integrated circuits
US7932545B2 (en)	2006-03-09	2011-04-26	Tela Innovations, Inc.	Semiconductor device and associated layouts including gate electrode level region having arrangement of six linear conductive segments with side-to-side spacing less than 360 nanometers
US7586800B1 (en)	2006-08-08	2009-09-08	Tela Innovations, Inc.	Memory timing apparatus and associated methods
US7979829B2 (en) *	2007-02-20	2011-07-12	Tela Innovations, Inc.	Integrated circuit cell library with cell-level process compensation technique (PCT) application and associated methods
US8286107B2 (en)	2007-02-20	2012-10-09	Tela Innovations, Inc.	Methods and systems for process compensation technique acceleration
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US8667443B2 (en)	2007-03-05	2014-03-04	Tela Innovations, Inc.	Integrated circuit cell library for multiple patterning
NZ583783A (en) *	2007-09-20	2012-10-26	Commw Scient Ind Res Org	Avian interferon-lambda polypeptide and genetic sequences encoding the same
JP5337043B2 (ja) *	2007-10-15	2013-11-06	中外製薬株式会社	異種タンパク質を高生産する細胞の作製方法
US8453094B2 (en)	2008-01-31	2013-05-28	Tela Innovations, Inc.	Enforcement of semiconductor structure regularity for localized transistors and interconnect
US7939443B2 (en)	2008-03-27	2011-05-10	Tela Innovations, Inc.	Methods for multi-wire routing and apparatus implementing same
JP5599395B2 (ja)	2008-07-16	2014-10-01	テライノヴェイションズインコーポレイテッド	動的アレイアーキテクチャにおけるセル位相整合及び配置の方法及びその実施
US9122832B2 (en)	2008-08-01	2015-09-01	Tela Innovations, Inc.	Methods for controlling microloading variation in semiconductor wafer layout and fabrication
GB0820631D0 (en) *	2008-11-11	2008-12-17	London School Hygiene & Tropical Medicine	Vectors
EP2239322A1 (en) *	2009-04-07	2010-10-13	Basf Se	Use of enzymes to reduce formaldehyde from formaldehyde-containing products
US8661392B2 (en)	2009-10-13	2014-02-25	Tela Innovations, Inc.	Methods for cell boundary encroachment and layouts implementing the Same
US9159627B2 (en)	2010-11-12	2015-10-13	Tela Innovations, Inc.	Methods for linewidth modification and apparatus implementing the same
EP2740485B1 (en)	2012-12-07	2018-10-31	Brightpulse Holding LTD.	Protein slurp-1 for use in the treatment of ocular diseases

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US4359415A (en) *	1981-06-03	1982-11-16	University Of Tennessee Research Corporation	Isolation of an antineoplastic protein fraction and an antineoplastic peptide fraction from human urine
US4470970A (en) *	1981-07-02	1984-09-11	Burzynski Stanislaw R	Purified antineoplaston fractions and methods of treating neoplastic disease
US5298604A (en) *	1992-07-27	1994-03-29	Sloane Nathan H	Parital primary amino acid sequence of the antineoplastic protein (ANUP); a cytokine present in granulocytes

1992
- 1992-12-22 IT ITRM920919A patent/IT1257184B/it active IP Right Grant
1993
- 1993-12-21 DE DE69332861T patent/DE69332861T2/de not_active Expired - Fee Related
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- 1993-12-21 EP EP94904167A patent/EP0675956B1/en not_active Expired - Lifetime
- 1993-12-21 DK DK94904167T patent/DK0675956T3/da active
- 1993-12-21 KR KR1019950702407A patent/KR100193107B1/ko not_active IP Right Cessation
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1995
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Publication number	Publication date
JP3025014B2 (ja)	2000-03-27
EP0675956A1 (en)	1995-10-11
RU2177480C2 (ru)	2001-12-27
DK0675956T3 (da)	2003-07-07
ITRM920919A1 (it)	1994-06-22
ES2193152T3 (es)	2003-11-01
EP0675956B1 (en)	2003-04-09
FI114478B (sv)	2004-10-29
WO1994014959A1 (en)	1994-07-07
US5908827A (en)	1999-06-01
PT675956E (pt)	2003-07-31
UA46702C2 (uk)	2002-06-17
CA2151156A1 (en)	1994-07-07
IT1257184B (it)	1996-01-10
ITRM920919A0 (it)	1992-12-22
FI20040608A (sv)	2004-04-29
AU690093B2 (en)	1998-04-23
NO952494D0 (no)	1995-06-21
ZA939621B (en)	1995-06-22
FI953091L (sv)	1995-06-21
JPH08509359A (ja)	1996-10-08
KR100193107B1 (ko)	1999-06-15
IL108149A0 (en)	1994-04-12
IL108149A (en)	2005-05-17
DE69332861D1 (de)	2003-05-15
ATE236976T1 (de)	2003-04-15
AU5833594A (en)	1994-07-19
FI953091A0 (sv)	1995-06-21
KR950704486A (ko)	1995-11-20
DE69332861T2 (de)	2004-01-29
FI116059B (sv)	2005-09-15
NO952494L (no)	1995-08-21

Publication	Publication Date	Title
NO315800B1 (no)	2003-10-27	Polypeptid fra urin med betegnelse komponent B, fremgangsmate for fremstilling av dette, DNA-molekyl, ekspresjonsvektor, vertscelle, pattedyrscelle, anvendelse av polypeptidet for fremstilling av et medikament samt farmasoytisk preparat
Yu et al.	1995	Molecular cloning and characterization of a cellular protein that interacts with the human immunodeficiency virus type 1 Tat transactivator and encodes a strong transcriptional activation domain
Rorsman et al.	1988	Structural characterization of the human platelet-derived growth factor A-chain cDNA and gene: alternative exon usage predicts two different precursor proteins
Gonzalez et al.	1989	A cluster of phosphorylation sites on the cyclic AMP-regulated nuclear factor CREB predicted by its sequence
Gradwohl et al.	1990	The second zinc-finger domain of poly (ADP-ribose) polymerase determines specificity for single-stranded breaks in DNA.
US5767250A (en)	1998-06-16	Complexes comprising truncated CMV gH polypeptides and escort proteins
EP0468337A1 (en)	1992-01-29	DNA coding for a human vasoconstrictive peptide and use thereof
Bunz et al.	1993	cDNAs encoding the large subunit of human replication factor C.
WO1996039426A9 (en)	1997-02-06	Hypoxia inducible factor-1 and method of use
WO1996039426A1 (en)	1996-12-12	Hypoxia inducible factor-1 and method of use
WO1989009277A1 (en)	1989-10-05	Mutant human angiogenin (angiogenesis factor with superior angiogenin activity) genes therefor and methods of expression
Matre et al.	1993	Molecular cloning of a functional human thyrotropin-releasing hormone receptor
WO1997005249A2 (en)	1997-02-13	Extension of a protein-protein interaction surface to inactivate the function of a cellular protein
Takekawa et al.	1992	Cloning and characterization of a human cDNA encoding a novel putative cytoplasmic protein-tyrosine-phosphatase
DE69430121T2 (de)	2002-08-01	Verfahren und zusammemsetzungen aehnlich den bindungsprotein des sterol-regulationselements
Chumakov et al.	1993	Localization of the c-ets-2 transactivation domain
WO1996029433A1 (en)	1996-09-26	Rest protein and dna
JPH04144684A (ja)	1992-05-19	エンドセリン受容体
JP2001511016A (ja)	2001-08-07	大動脈特異的および横紋筋特異的な筋肉細胞におけるアイソフォームをコードする単一遺伝子、およびその使用
US6355775B1 (en)	2002-03-12	Transcriptional inhibitor
US6444801B1 (en)	2002-09-03	Transcriptional inhibitor protein and the encoding DNA
Cook et al.	1998	Expression and Purification of Recombinant Tick Anticoagulant Peptide (Y1W/D10R) Double Mutant Secreted bySaccharomyces cerevisiae
US5914246A (en)	1999-06-22	Multiple drug resistance gene of Aspergillus fumigatus
AU1391700A (en)	2000-06-19	Polypeptides capable of interacting with the human topoisomerase iii alpha
JPH03123487A (ja)	1991-05-27	新規ポリペプチドの製造法

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