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FI114478B - Förfarande för framställning av protein kallat komponent B ur urin - Google Patents

Förfarande för framställning av protein kallat komponent B ur urin Download PDF

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Publication number
FI114478B
FI114478B FI953091A FI953091A FI114478B FI 114478 B FI114478 B FI 114478B FI 953091 A FI953091 A FI 953091A FI 953091 A FI953091 A FI 953091A FI 114478 B FI114478 B FI 114478B
Authority
FI
Finland
Prior art keywords
resin
urine
fraction
acetate buffer
component
Prior art date
Application number
FI953091A
Other languages
English (en)
Finnish (fi)
Other versions
FI953091L (sv
FI953091A0 (sv
Inventor
Antonino Sirna
Original Assignee
Applied Research Systems
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Applied Research Systems filed Critical Applied Research Systems
Publication of FI953091L publication Critical patent/FI953091L/sv
Publication of FI953091A0 publication Critical patent/FI953091A0/sv
Application granted granted Critical
Publication of FI114478B publication Critical patent/FI114478B/sv

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4702—Regulators; Modulating activity
    • C07K14/4703—Inhibitors; Suppressors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/71—Receptors; Cell surface antigens; Cell surface determinants for growth factors; for growth regulators
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Toxicology (AREA)
  • Biomedical Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • Pain & Pain Management (AREA)
  • Hematology (AREA)
  • Physics & Mathematics (AREA)
  • Rheumatology (AREA)
  • Diabetes (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Saccharide Compounds (AREA)

Claims (2)

1. Ett förfarande för att framställa en polypeptid, som innehäller en sekvens SEKV. ID NO: 1 peptidsekvens, dess salter, funktionella derivat, förstadier och aktiva fraktioner eller en blandning av dessa, kännetecknat av, att framställningsförfarandet innehäller steg där man: a) läter urin adsorberas vid kaolin i surt pH och urlakar detsamma i ammoniak b) eluderar fraktionen (a) med Bio Rex 70-harts med ammoniak c) eluderar fraktionen (b) med DEAE Sepharose-harts med acetatbuffer d) eluderar fraktionen (c) med CM Sepharose-harts med acetatbuffer e) eluderar fraktionen (d) med HPLC C18-harts med en blandning av acetatbuffer och acetonitril f) eluderar fraktionen (e) med DE-52-harts med acetatbuffer ;*·,· g) eluderar fraktionen (f) med D-Zephyr-harts med acetatbuffer h) eluderar fraktionen (g) med HPLC C18-harts med en : blandning av trifluorättiksyra i vattenlösning och * # : : : acetonitril * * « · ·*,·* i) eluderar fraktionen (h) med D-Zephyr-harts med acetatbuffer. « * · » * » »
2. Förfarande enligt patentkrav 1, kännetecknat av, att ; urinet är humanurin. * · >
FI953091A 1992-12-22 1995-06-21 Förfarande för framställning av protein kallat komponent B ur urin FI114478B (sv)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
ITRM920919A IT1257184B (it) 1992-12-22 1992-12-22 Preparato ad attivita' antinfiammatoria, anticoagulante e antitumorale
ITRM920919 1992-12-22
PCT/EP1993/003645 WO1994014959A1 (en) 1992-12-22 1993-12-21 New protein from urine named component b
EP9303645 1993-12-21

Publications (3)

Publication Number Publication Date
FI953091L FI953091L (sv) 1995-06-21
FI953091A0 FI953091A0 (sv) 1995-06-21
FI114478B true FI114478B (sv) 2004-10-29

Family

ID=11401349

Family Applications (2)

Application Number Title Priority Date Filing Date
FI953091A FI114478B (sv) 1992-12-22 1995-06-21 Förfarande för framställning av protein kallat komponent B ur urin
FI20040608A FI116059B (sv) 1992-12-22 2004-04-29 Analogiförfarande för framställning av ett rekombinantprotein kallat komponent B samt DNA-molekyl, expressionsvektor och värdcell

Family Applications After (1)

Application Number Title Priority Date Filing Date
FI20040608A FI116059B (sv) 1992-12-22 2004-04-29 Analogiförfarande för framställning av ett rekombinantprotein kallat komponent B samt DNA-molekyl, expressionsvektor och värdcell

Country Status (19)

Country Link
US (1) US5908827A (sv)
EP (1) EP0675956B1 (sv)
JP (1) JP3025014B2 (sv)
KR (1) KR100193107B1 (sv)
AT (1) ATE236976T1 (sv)
AU (1) AU690093B2 (sv)
CA (1) CA2151156A1 (sv)
DE (1) DE69332861T2 (sv)
DK (1) DK0675956T3 (sv)
ES (1) ES2193152T3 (sv)
FI (2) FI114478B (sv)
IL (1) IL108149A (sv)
IT (1) IT1257184B (sv)
NO (1) NO315800B1 (sv)
PT (1) PT675956E (sv)
RU (1) RU2177480C2 (sv)
UA (1) UA46702C2 (sv)
WO (1) WO1994014959A1 (sv)
ZA (1) ZA939621B (sv)

Families Citing this family (41)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EA001077B1 (ru) * 1996-04-24 2000-10-30 Апплайд Резеч Системз Арс Холдинг Н.В. Компонент в, используемый в качестве рубцующего агента, содержащая его фармацевтическая композиция и способ лечения
AU8536698A (en) * 1997-06-09 1998-12-30 Wolf-Georg Forssmann Lus-1 human protein, its production and use
DE69903037T2 (de) * 1998-07-09 2003-06-05 Applied Research Systems Ars Holding N.V., Curacao Komponente b als angiogenes mittel zusammen mit humanen wachstumsfaktoren
US6962700B1 (en) * 2000-09-13 2005-11-08 Atopix Pharmaceuticals Corporation Method of manufacturing immune globulin
ATE494903T1 (de) * 2003-04-16 2011-01-15 Merck Serono Sa Verwendung von slurp-1 zur behandlung von krankheiten, die durch störungen der acetylcholinerezeptoren ausgelöst werden
US7343581B2 (en) * 2005-06-27 2008-03-11 Tela Innovations, Inc. Methods for creating primitive constructed standard cells
US7590968B1 (en) 2006-03-01 2009-09-15 Tela Innovations, Inc. Methods for risk-informed chip layout generation
US8225261B2 (en) 2006-03-09 2012-07-17 Tela Innovations, Inc. Methods for defining contact grid in dynamic array architecture
US8839175B2 (en) 2006-03-09 2014-09-16 Tela Innovations, Inc. Scalable meta-data objects
US8541879B2 (en) 2007-12-13 2013-09-24 Tela Innovations, Inc. Super-self-aligned contacts and method for making the same
US7956421B2 (en) 2008-03-13 2011-06-07 Tela Innovations, Inc. Cross-coupled transistor layouts in restricted gate level layout architecture
US8225239B2 (en) 2006-03-09 2012-07-17 Tela Innovations, Inc. Methods for defining and utilizing sub-resolution features in linear topology
US8245180B2 (en) 2006-03-09 2012-08-14 Tela Innovations, Inc. Methods for defining and using co-optimized nanopatterns for integrated circuit design and apparatus implementing same
US7446352B2 (en) 2006-03-09 2008-11-04 Tela Innovations, Inc. Dynamic array architecture
US8247846B2 (en) 2006-03-09 2012-08-21 Tela Innovations, Inc. Oversized contacts and vias in semiconductor chip defined by linearly constrained topology
US9230910B2 (en) 2006-03-09 2016-01-05 Tela Innovations, Inc. Oversized contacts and vias in layout defined by linearly constrained topology
US8448102B2 (en) 2006-03-09 2013-05-21 Tela Innovations, Inc. Optimizing layout of irregular structures in regular layout context
US9009641B2 (en) 2006-03-09 2015-04-14 Tela Innovations, Inc. Circuits with linear finfet structures
US9035359B2 (en) 2006-03-09 2015-05-19 Tela Innovations, Inc. Semiconductor chip including region including linear-shaped conductive structures forming gate electrodes and having electrical connection areas arranged relative to inner region between transistors of different types and associated methods
US7943967B2 (en) 2006-03-09 2011-05-17 Tela Innovations, Inc. Semiconductor device and associated layouts including diffusion contact placement restriction based on relation to linear conductive segments
US8658542B2 (en) 2006-03-09 2014-02-25 Tela Innovations, Inc. Coarse grid design methods and structures
US8653857B2 (en) 2006-03-09 2014-02-18 Tela Innovations, Inc. Circuitry and layouts for XOR and XNOR logic
US9563733B2 (en) 2009-05-06 2017-02-07 Tela Innovations, Inc. Cell circuit and layout with linear finfet structures
US7763534B2 (en) 2007-10-26 2010-07-27 Tela Innovations, Inc. Methods, structures and designs for self-aligning local interconnects used in integrated circuits
US7932545B2 (en) 2006-03-09 2011-04-26 Tela Innovations, Inc. Semiconductor device and associated layouts including gate electrode level region having arrangement of six linear conductive segments with side-to-side spacing less than 360 nanometers
US7586800B1 (en) 2006-08-08 2009-09-08 Tela Innovations, Inc. Memory timing apparatus and associated methods
US7979829B2 (en) * 2007-02-20 2011-07-12 Tela Innovations, Inc. Integrated circuit cell library with cell-level process compensation technique (PCT) application and associated methods
US8286107B2 (en) 2007-02-20 2012-10-09 Tela Innovations, Inc. Methods and systems for process compensation technique acceleration
US7888705B2 (en) 2007-08-02 2011-02-15 Tela Innovations, Inc. Methods for defining dynamic array section with manufacturing assurance halo and apparatus implementing the same
US8667443B2 (en) 2007-03-05 2014-03-04 Tela Innovations, Inc. Integrated circuit cell library for multiple patterning
NZ583783A (en) * 2007-09-20 2012-10-26 Commw Scient Ind Res Org Avian interferon-lambda polypeptide and genetic sequences encoding the same
JP5337043B2 (ja) * 2007-10-15 2013-11-06 中外製薬株式会社 異種タンパク質を高生産する細胞の作製方法
US8453094B2 (en) 2008-01-31 2013-05-28 Tela Innovations, Inc. Enforcement of semiconductor structure regularity for localized transistors and interconnect
US7939443B2 (en) 2008-03-27 2011-05-10 Tela Innovations, Inc. Methods for multi-wire routing and apparatus implementing same
JP5599395B2 (ja) 2008-07-16 2014-10-01 テラ イノヴェイションズ インコーポレイテッド 動的アレイアーキテクチャにおけるセル位相整合及び配置の方法及びその実施
US9122832B2 (en) 2008-08-01 2015-09-01 Tela Innovations, Inc. Methods for controlling microloading variation in semiconductor wafer layout and fabrication
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US4359415A (en) * 1981-06-03 1982-11-16 University Of Tennessee Research Corporation Isolation of an antineoplastic protein fraction and an antineoplastic peptide fraction from human urine
US4470970A (en) * 1981-07-02 1984-09-11 Burzynski Stanislaw R Purified antineoplaston fractions and methods of treating neoplastic disease
US5298604A (en) * 1992-07-27 1994-03-29 Sloane Nathan H Parital primary amino acid sequence of the antineoplastic protein (ANUP); a cytokine present in granulocytes

Also Published As

Publication number Publication date
JP3025014B2 (ja) 2000-03-27
NO315800B1 (no) 2003-10-27
EP0675956A1 (en) 1995-10-11
RU2177480C2 (ru) 2001-12-27
DK0675956T3 (da) 2003-07-07
ITRM920919A1 (it) 1994-06-22
ES2193152T3 (es) 2003-11-01
EP0675956B1 (en) 2003-04-09
WO1994014959A1 (en) 1994-07-07
US5908827A (en) 1999-06-01
PT675956E (pt) 2003-07-31
UA46702C2 (uk) 2002-06-17
CA2151156A1 (en) 1994-07-07
IT1257184B (it) 1996-01-10
ITRM920919A0 (it) 1992-12-22
FI20040608A (sv) 2004-04-29
AU690093B2 (en) 1998-04-23
NO952494D0 (no) 1995-06-21
ZA939621B (en) 1995-06-22
FI953091L (sv) 1995-06-21
JPH08509359A (ja) 1996-10-08
KR100193107B1 (ko) 1999-06-15
IL108149A0 (en) 1994-04-12
IL108149A (en) 2005-05-17
DE69332861D1 (de) 2003-05-15
ATE236976T1 (de) 2003-04-15
AU5833594A (en) 1994-07-19
FI953091A0 (sv) 1995-06-21
KR950704486A (ko) 1995-11-20
DE69332861T2 (de) 2004-01-29
FI116059B (sv) 2005-09-15
NO952494L (no) 1995-08-21

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