NO301081B1 - Subtilisinmutant med endret proteolytisk aktivitet eller stabilitet, DNA som koder for mutanter, ekspresjonsvektor, vertscelle samt enzymatisk renseblanding i stand til å nedbryte proteiner - Google Patents

Subtilisinmutant med endret proteolytisk aktivitet eller stabilitet, DNA som koder for mutanter, ekspresjonsvektor, vertscelle samt enzymatisk renseblanding i stand til å nedbryte proteiner Download PDF

Info

Publication number: NO301081B1
Authority: NO; Norway
Prior art keywords: subtilisin; amino acid; bacillus; mutant; precursor
Prior art date: 1989-10-31

Application number

NO912567A

Other languages

English (en)

Norwegian (no)

Other versions

NO912567L (no

NO912567D0 (no

Inventor

Robert Mark Caldwell

David Aaron Estell

Thomas Paul Graycar

Original Assignee

Genencor Int

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1989-10-31

Filing date

1991-06-28

Publication date

1997-09-08

Family has litigation

First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=27028376&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=NO301081(B1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.

1991-06-28 Application filed by Genencor Int filed Critical Genencor Int

1991-06-28 Publication of NO912567L publication Critical patent/NO912567L/no

1991-06-28 Publication of NO912567D0 publication Critical patent/NO912567D0/no

1997-09-08 Publication of NO301081B1 publication Critical patent/NO301081B1/no

Links

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Classifications

- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
- C11D3/38609—Protease or amylase in solid compositions only
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/18—Carboxylic ester hydrolases (3.1.1)
- C12N9/20—Triglyceride splitting, e.g. by means of lipase
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
- C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6409—Fatty acids
- C12P7/6418—Fatty acids by hydrolysis of fatty acid esters
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus

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Chemical & Material Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Health & Medical Sciences (AREA)
Engineering & Computer Science (AREA)
Organic Chemistry (AREA)
Genetics & Genomics (AREA)
Wood Science & Technology (AREA)
Zoology (AREA)
Bioinformatics & Cheminformatics (AREA)
Biotechnology (AREA)
General Engineering & Computer Science (AREA)
Biomedical Technology (AREA)
General Health & Medical Sciences (AREA)
Microbiology (AREA)
Biochemistry (AREA)
Molecular Biology (AREA)
Medicinal Chemistry (AREA)
Oil, Petroleum & Natural Gas (AREA)
Chemical Kinetics & Catalysis (AREA)
Physics & Mathematics (AREA)
Plant Pathology (AREA)
Biophysics (AREA)
General Chemical & Material Sciences (AREA)
Crystallography & Structural Chemistry (AREA)
Tropical Medicine & Parasitology (AREA)
Virology (AREA)
Enzymes And Modification Thereof (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Detergent Compositions (AREA)
Peptides Or Proteins (AREA)

NO912567A 1989-10-31 1991-06-28 Subtilisinmutant med endret proteolytisk aktivitet eller stabilitet, DNA som koder for mutanter, ekspresjonsvektor, vertscelle samt enzymatisk renseblanding i stand til å nedbryte proteiner NO301081B1 (no)

Applications Claiming Priority (3)

Application Number	Priority Date	Filing Date	Title
US42988289A	1989-10-31	1989-10-31
US07/600,430 US5185258A (en)	1984-05-29	1990-10-19	Subtilisin mutants
PCT/US1990/006084 WO1991006637A1 (en)	1989-10-31	1990-10-26	Subtilisin mutants

Publications (3)

Publication Number	Publication Date
NO912567L NO912567L (no)	1991-06-28
NO912567D0 NO912567D0 (no)	1991-06-28
NO301081B1 true NO301081B1 (no)	1997-09-08

Family

ID=27028376

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
NO912567A NO301081B1 (no)	1989-10-31	1991-06-28	Subtilisinmutant med endret proteolytisk aktivitet eller stabilitet, DNA som koder for mutanter, ekspresjonsvektor, vertscelle samt enzymatisk renseblanding i stand til å nedbryte proteiner

Country Status (21)

Country	Link
US (1)	US5185258A (pt)
EP (2)	EP0775749B2 (pt)
JP (1)	JP3335623B2 (pt)
KR (1)	KR970004938B1 (pt)
CN (1)	CN1052897A (pt)
AR (1)	AR244339A1 (pt)
AT (2)	ATE297997T1 (pt)
AU (1)	AU633376B2 (pt)
BR (1)	BR9006993A (pt)
CA (1)	CA2044630C (pt)
DE (2)	DE69032852T2 (pt)
DK (2)	DK0451244T3 (pt)
ES (2)	ES2242963T5 (pt)
FI (1)	FI103052B1 (pt)
IE (1)	IE62771B1 (pt)
MA (1)	MA22573A1 (pt)
NO (1)	NO301081B1 (pt)
NZ (1)	NZ235883A (pt)
PT (1)	PT95741B (pt)
TR (1)	TR25991A (pt)
WO (1)	WO1991006637A1 (pt)

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- 1990-10-26 AU AU66341/90A patent/AU633376B2/en not_active Expired
- 1990-10-26 WO PCT/US1990/006084 patent/WO1991006637A1/en active IP Right Grant
- 1990-10-26 BR BR909006993A patent/BR9006993A/pt not_active IP Right Cessation
- 1990-10-26 DE DE69032852T patent/DE69032852T2/de not_active Expired - Lifetime
- 1990-10-26 JP JP51505890A patent/JP3335623B2/ja not_active Expired - Lifetime
- 1990-10-26 DK DK90915958T patent/DK0451244T3/da active
- 1990-10-26 DK DK96120247T patent/DK0775749T4/da active
- 1990-10-26 AT AT96120247T patent/ATE297997T1/de not_active IP Right Cessation
- 1990-10-26 KR KR1019910700677A patent/KR970004938B1/ko not_active IP Right Cessation
- 1990-10-26 ES ES96120247T patent/ES2242963T5/es not_active Expired - Lifetime
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- 1990-10-30 PT PT95741A patent/PT95741B/pt not_active IP Right Cessation
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1991
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Publication number	Publication date
CN1052897A (zh)	1991-07-10
EP0775749A1 (en)	1997-05-28
DE69034195T3 (de)	2009-10-22
AU6634190A (en)	1991-05-31
AU633376B2 (en)	1993-01-28
DE69032852T2 (de)	1999-05-20
CA2044630C (en)	2000-05-30
IE903905A1 (en)	1991-05-08
IE62771B1 (en)	1995-02-22
DE69034195T2 (de)	2006-03-23
EP0451244A1 (en)	1991-10-16
JPH04505263A (ja)	1992-09-17
DK0775749T3 (da)	2005-09-12
CA2044630A1 (en)	1991-05-01
US5185258A (en)	1993-02-09
MA22573A1 (fr)	1993-04-01
PT95741B (pt)	1997-10-31
EP0775749B2 (en)	2009-04-01
DK0775749T4 (da)	2009-07-20
ATE297997T1 (de)	2005-07-15
TR25991A (tr)	1993-11-01
FI103052B (fi)	1999-04-15
ES2242963T3 (es)	2005-11-16
WO1991006637A1 (en)	1991-05-16
JP3335623B2 (ja)	2002-10-21
AR244339A1 (es)	1993-10-29
ES2125224T3 (es)	1999-03-01
DK0451244T3 (da)	1999-08-23
ATE174961T1 (de)	1999-01-15
BR9006993A (pt)	1991-11-26
DE69032852D1 (de)	1999-02-04
FI913166A0 (fi)	1991-06-28
ES2242963T5 (es)	2009-08-27
EP0451244B9 (en)	2005-01-26
KR970004938B1 (ko)	1997-04-10
NO912567L (no)	1991-06-28
NZ235883A (en)	1992-09-25
DE69034195D1 (de)	2005-07-21
NO912567D0 (no)	1991-06-28
PT95741A (pt)	1991-09-13
EP0451244B1 (en)	1998-12-23
FI103052B1 (fi)	1999-04-15
EP0451244A4 (en)	1992-06-03
EP0775749B1 (en)	2005-06-15

Publication	Publication Date	Title
NO301081B1 (no)	1997-09-08	Subtilisinmutant med endret proteolytisk aktivitet eller stabilitet, DNA som koder for mutanter, ekspresjonsvektor, vertscelle samt enzymatisk renseblanding i stand til å nedbryte proteiner
US5204015A (en)	1993-04-20	Subtilisin mutants
RU2136756C1 (ru)	1999-09-10	Модифицированный субтилизин, днк, кодирующая модифицированный субтилизин, вектор экспрессии, кодирующий днк, и штамм культуры клетки хозяина
JP5612026B2 (ja)	2014-10-22	洗剤で使用される改変された正味の特性を有する複数置換プロテアーゼ変異体
US6300116B1 (en)	2001-10-09	Modified protease having improved autoproteolytic stability
EP1523553B1 (en)	2009-12-02	Multiply-substituted protease variants
AU2003277836A1 (en)	2004-06-07	Subtilase variants
EP2287321B1 (en)	2014-08-13	Multiply-substituted protease variants
KR100767710B1 (ko)	2007-10-17	위치 97 및 98 사이에 적어도 하나의 추가의 아미노산잔기를 가지는 ｉ-ｓ1 및 ｉ-ｓ2 아군의 서브틸라제 효소
EP1183342A1 (en)	2002-03-06	Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additional amino acid residue between positions 128 and 129
MXPA00003807A (en)	2001-06-26	Multiply-substituted protease variants
MXPA00003801A (en)	2001-06-26	Multiply-substituted protease variants
MXPA00003806A (en)	2001-05-07	Multiply-substituted protease variants with altered net charge for use in detergents

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